1. Acer tegmentosum extract-mediated silver nanoparticles loaded chitosan/alginic acid scaffolds enhance healing of E. coli-infected wounds.
- Author
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Li Z, Saravanakumar K, Yao L, Kim Y, Choi SY, Yoo G, Keon K, Lee CM, Youn B, Lee D, and Cho N
- Subjects
- Animals, Mice, NIH 3T3 Cells, Escherichia coli Infections drug therapy, Tissue Scaffolds chemistry, Chitosan chemistry, Chitosan pharmacology, Metal Nanoparticles chemistry, Silver chemistry, Silver pharmacology, Wound Healing drug effects, Escherichia coli drug effects, Acer chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Alginates chemistry, Alginates pharmacology
- Abstract
This work developed Acer tegmentosum extract-mediated silver nanoparticles (AgNPs) loaded chitosan (CS)/alginic acid (AL) scaffolds (CS/AL-AgNPs) to enhance the healing of E. coli-infected wounds. The SEM-EDS and XRD results revealed the successful formation of the CS/AL-AgNPs. FTIR analysis evidenced that the anionic group of AL (-COO-) and cationic amine groups of CS (-NH
3 + ) were ionically crosslinked to form scaffold (CS/AL). The CS/AL-AgNPs exhibited significant antimicrobial activity against both Gram-positive (G+) and Gram-negative (G-) bacterial pathogens, while being non-toxic to red blood cells (RBCs), the hen's egg chorioallantoic membrane (HET-CAM), and a non-cancerous cell line (NIH3T3). Treatment with CS/AL-AgNPs significantly accelerated the healing of E. coli-infected wounds by regulating the collagen deposition and blood parameters as evidenced by in vivo experiments. Overall, these findings suggest that CS/AL-AgNPs are promising for the treatment of infected wounds., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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