5 results on '"Hennager, Steven G"'
Search Results
2. Comparison of Buffered, Acidified Plate Antigen to Standard Serologic Tests for the Detection of Serum Antibodies to Brucella abortus in Elk (Cervus canadensis).
- Author
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Clarke PR, Edwards WH, Hennager SG, Block JF, Yates AM, Ebel E, Knopp DJ, Fuentes-Sanchez A, Jennings-Gaines J, Kientz RL, and Simunich M
- Subjects
- Agglutination Tests methods, Animals, Antibodies, Bacterial blood, Brucellosis diagnosis, Brucellosis immunology, Deer blood, Deer immunology, Serologic Tests methods, Agglutination Tests veterinary, Antibodies, Bacterial immunology, Antigens, Bacterial immunology, Brucella abortus immunology, Brucellosis veterinary, Deer microbiology, Serologic Tests veterinary
- Abstract
Brucellosis (caused by the bacterium Brucella abortus) is a zoonotic disease endemic in wild elk (Cervus canadensis) of the Greater Yellowstone Ecosystem, US. Because livestock and humans working with elk or livestock are at risk, validated tests to detect the B. abortus antibody in elk are needed. Using the κ-statistic, we evaluated the buffered, acidified plate antigen (BAPA) assay for agreement with the results of the four serologic tests (card test [card], complement fixation test [CF], rivanol precipitation plate agglutination test [RIV], standard plate agglutination test [SPT]) that are approved by the US Department of Agriculture for the detection of the B. abortus antibody in elk. From 2006 to 2010, serum samples collected from elk within B. abortus-endemic areas (n = 604) and nonendemic areas (n = 707) and from elk culture-positive for B. abortus (n = 36) were split and blind tested by four elk serum diagnostic laboratories. κ-Values showed a high degree of agreement for the card (0.876), RIV (0.84), and CF (0.774) test pairings and moderate agreement for the SPT (0.578). Sensitivities for the BAPA, card, RIV, CF, and SPT were 0.859, 0.839, 0.899, 1.00, and 0.813, whereas specificities were 0.986, 0.993, 0.986, 0.98, and 0.968, respectively. The positive predictive values and the negative predictive values were calculated for 2.6%, 8.8%, and 16.2% prevalence levels. These findings suggest the BAPA test is a suitable screening test for the B. abortus antibodies in elk.
- Published
- 2015
- Full Text
- View/download PDF
3. Babesia equi-induced anemia in a Quarter Horse and subsequent regulatory response.
- Author
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Beard LA, Pelzel AM, Rush BR, Wright AM, Galgut BI, Hennager SG, King AO, and Traub-Dargatz JL
- Subjects
- Anemia etiology, Anemia microbiology, Animals, Babesiosis complications, Babesiosis microbiology, Horse Diseases microbiology, Horse Diseases pathology, Horses, Male, Running, Sports, Anemia veterinary, Babesia classification, Babesiosis veterinary, Horse Diseases etiology
- Abstract
Case Description: A 7-year-old Quarter Horse gelding used for unsanctioned racing was examined because of fever and anorexia., Clinical Findings: Physical examination revealed fever, tachycardia, and tachypnea. Results of a CBC indicated anemia and mild thrombocytopenia. Results of microscopic examination of a blood smear indicated piroplasms in erythrocytes, consistent with Babesia spp. Regulatory authorities were contacted, and results of serologic testing at the National Veterinary Services Laboratories confirmed acute Babesia equi infection., Treatment and Outcome: Equids on the home premises of the index horse were placed under quarantine. Those equids were tested for piroplasmosis, and 6 of 63 horses had positive results for B equi. Another horse that had previously been housed on the index premises also had positive results for B equi. Competent tick vectors for piroplasmosis organisms were not identified. All 8 horses with piroplasmosis were Quarter Horses that participated in unsanctioned racing and were trained by the same person. Two of the horses were illegally removed from the index premises; these 2 horses and the other horse with piroplasmosis that was previously housed on the index premises could not be found. The other 5 horses with piroplasmosis were euthanized. Investigators concluded that transmission of B equi among horses was most likely iatrogenic., Clinical Relevance: The United States has been considered piroplasmosis free. However, veterinarians should consider piroplasmosis in horses with signalments and clinical signs similar to those of the index horse of this report. Regulatory authorities should be contacted regarding horses in which piroplasmosis is suspected.
- Published
- 2013
- Full Text
- View/download PDF
4. Outbreak of equine piroplasmosis in Florida.
- Author
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Short MA, Clark CK, Harvey JW, Wenzlow N, Hawkins IK, Allred DR, Knowles DP, Corn JL, Grause JF, Hennager SG, Kitchen DL, and Traub-Dargatz JL
- Subjects
- Animal Husbandry, Animals, Babesia isolation & purification, Babesiosis epidemiology, Babesiosis mortality, Babesiosis transmission, Female, Florida epidemiology, Horse Diseases mortality, Horse Diseases parasitology, Horse Diseases transmission, Horses, Male, Babesiosis veterinary, Disease Outbreaks veterinary, Horse Diseases epidemiology
- Abstract
Case Description: A 7-year-old Quarter Horse gelding was hospitalized in Ocala, Fla, because of lethargy, fever, anorexia, and swelling of distal aspects of the limbs. A tentative diagnosis of equine piroplasmosis (EP) was made on the basis of examination of a blood smear. The case was reported to the Florida State Veterinarian, and infection with Babesia equi was confirmed. The subsequent investigation included quarantine and testing of potentially exposed horses for B equi and Babesia caballi infections, tick surveillance, and owner-agent interviews., Clinical Findings: 210 horses on 25 premises were tested for infection with EP pathogens. Twenty B equi-infected horses on 7 premises were identified; no horses tested positive for B caballi. Seven horses, including the index case, had clinical findings consistent with EP Dermacentor variabilis was considered the only potential tick vector for B equi collected, and all D variabilis specimens tested negative for Babesia organisms via PCR assay. Results of the epidemiological investigation suggested that B equi was spread by use of shared needles and possibly blood transfusions. All horses that tested positive were involved in nonsanctioned Quarter Horse racing, and management practices were thought to pose substantial risk of transmission of blood-borne pathogens., Treatment and Outcome: Final outcome of B equi-infected horses was euthanasia, death from undetermined causes, or shipment to a US federal research facility., Clinical Relevance: This investigation highlights the importance of collaboration between private veterinary practitioners, state veterinary diagnostic laboratories, and regulatory officials in the recognition, containment, and eradication of foreign animal disease.
- Published
- 2012
- Full Text
- View/download PDF
5. Equine piroplasmosis associated with Amblyomma cajennense Ticks, Texas, USA.
- Author
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Scoles GA, Hutcheson HJ, Schlater JL, Hennager SG, Pelzel AM, and Knowles DP
- Subjects
- Animals, Babesiosis diagnosis, Babesiosis epidemiology, Babesiosis transmission, Female, Horse Diseases parasitology, Horse Diseases transmission, Horses, Male, Texas epidemiology, Babesiosis veterinary, Disease Outbreaks veterinary, Horse Diseases epidemiology, Ixodidae
- Abstract
We report an outbreak of equine piroplasmosis in southern Texas, USA, in 2009. Infection prevalence reached 100% in some areas (292 infected horses). Amblyomma cajennense was the predominant tick and experimentally transmitted Theileria equi to an uninfected horse. We suggest that transmission by this tick species played a role in this outbreak.
- Published
- 2011
- Full Text
- View/download PDF
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