44 results on '"Campos DM"'
Search Results
2. In Silico Evaluation of the Binding Energies of Androgen Receptor Agonists in Wild-Type and Mutational Models.
- Author
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Albuquerque ACC, Bezerra KS, de Fátima Vianna J, Batista SO, de Lima Neto JX, de Oliveira Campos DM, Oliveira JIN, Galvão DS, and Fulco UL
- Subjects
- Male, Humans, Ligands, Dihydrotestosterone chemistry, Testosterone chemistry, Mutation, Androgens, Receptors, Androgen metabolism
- Abstract
Anabolic androgenic steroids (AAS) are substances with androgenic and anabolic characteristics. Among the many side effects of hormone therapy with AAS, the following stand out: heart problems, adrenal gland disorders, aggressive behavior, increased risk of prostate cancer, problems related to lack of libido and impotence. Such substances vary in the relationship between androgenic activity, and the activation of the androgen receptor (AR) is of fundamental importance for the singularity of the action of each AAS. In this sense, our study evaluates the aspects that comprise the interactions of testosterone agonists (TES), dihydrotestosterone (DHT) and tetrahydrogestrinone (THG) in complex with the AR. In addition, we also evaluated the impact of ligand-receptor affinity differences in a mutation model. We apply computational techniques based on density functional theory (DFT) and use, as methodology, Molecular Fractionation with Conjugate Caps (MFCC). The energetic specificities present in the interaction between the analyzed complexes attest that the highest affinity with the AR receptor is found for AR-THG, followed by AR-DHT, AR-TES and AR-T877A-DHT, respectively. Our results also show the differences and equivalences between the different agonists, in addition to evaluating the difference between the DHT ligand in complex with the wild-type and mutant receptor, presenting the main amino acid residues that involve the interaction with the ligands. The computational methodology used proves to be an operative and sophisticated choice to help in the search for pharmacological agents for various therapies that have androgen as a target.
- Published
- 2023
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3. Effectiveness of COVID-19 vaccines against Omicron variant.
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de Oliveira Campos DM, da Silva MK, Silva de Oliveira CB, Fulco UL, and Nobre Oliveira JI
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- Antibodies, Neutralizing, Antibodies, Viral, Humans, SARS-CoV-2, COVID-19 prevention & control, COVID-19 Vaccines therapeutic use
- Published
- 2022
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4. Effects of Aquatic Physical Exercise on Motor Risk Factors for Falls in Older People During the COVID-19 Pandemic: A Randomized Controlled Trial.
- Author
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Ferreira DL, Christofoletti G, Campos DM, Janducci AL, Candanedo MJBL, and Ansai JH
- Subjects
- Humans, Aged, Pandemics prevention & control, Postural Balance physiology, COVID-19 Testing, Time and Motion Studies, Exercise Therapy, Exercise physiology, Risk Factors, Accidental Falls prevention & control, COVID-19 epidemiology
- Abstract
Objective: The aim of this study was to assess the effects of aquatic training on motor risk factors for falls in older people during the COVID-19 pandemic., Methods: A randomized controlled trial was carried out with older people, divided into an aquatic training group (ATG) (n = 24) and a control group (CG) (n = 25). Muscle strength was assessed by the 5-Times Sit-to-Stand Test, mobility by the simple and dual-task Timed Up and Go Test, and postural stability through stabilometric data (force platform). The CG received monthly calls to monitor general health. The ATG carried out training lasting 16 weeks, with two 1-hour sessions per week., Results: Both groups improved muscular strength and cognitive-motor tasks, and they performed a dual task with fewer errors in the secondary task after 16 weeks regardless of the pandemic and COVID-19 diagnosis. There was a significant decrease in the area of center of pressure displacement in the tandem posture with eyes closed in the CG. When analyzing participants who adhered at least 50% to the intervention, the ATG significantly reduced the number of steps on the Timed Up and Go Test performance. Both groups improved muscular strength and cognitive-motor tasks and increased the cognitive task cost. In the CG, there was a significant decrease in the mean amplitude of the anteroposterior center of pressure displacement in the feet together with eyes open., Conclusion: We found that aquatic physical exercise presented positive effects on some potentially modifiable motor risk factors for falls (mobility and muscle strength) regardless of the COVID-19 pandemic and COVID-19 diagnosis, especially among people who adhered to the intervention., (Copyright © 2022. Published by Elsevier Inc.)
- Published
- 2022
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5. Effects of aquatic physical exercise on neuropsychological factors in older people: A systematic review.
- Author
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Campos DM, Ferreira DL, Gonçalves GH, Farche ACS, de Oliveira JC, and Ansai JH
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- Aged, Aged, 80 and over, Exercise, Exercise Therapy, Fear, Female, Humans, Accidental Falls, Quality of Life
- Abstract
Background: . There is a lack of standardized protocols and clinical trials for older adults involving neuropsychological factors in the literature. Furthermore, no systematic review has been published investigating this theme., Purpose: . The purpose of this systematic review was to analyze the effects of aquatic physical exercise on neuropsychological factors in older adults., Methods: . A systematic review (CRD42020176899) was conducted, using articles from Pubmed, Web of Science, Scopus, Cochrane Library, Science Direct and Medline published until March 2020 (without limit of previous year). Eligibility criteria for selecting studies were: clinical trials; samples with a mean age of 65 years old and over; articles involving aquatic physical exercise; and presenting neuropsychological outcomes (behavior, cognition, psychological, mental health)., Results and Discussion: . The search yielded 801 records and 16 studies were included, totaling: 1707 older adults, with a mean age of 71.3 years old (range of mean ages in the studies: 65.3 to 88.4 years old) and a predominance of women. Aquatic physical exercise showed positive results in the mental component of quality of life, fear of falling, mood, anxiety and internal health locus of control in healthy sedentary older adults. Only one study out of 5 carried out with older adults with osteoarthritis showed improvements in psychological well-being after aquatic intervention. Older women with osteopenia or osteoporosis showed improvements in the mental component of quality of life. Studies on dementia showed a significant improvement in behavioral and psychological symptoms after aquatic intervention and no effect on depressive symptoms., Conclusion: . Based on the results of this systematic review, aquatic physical exercise has positive effects on quality of life, fear of falling, cognitive functions, mood, anxiety and internal health locus of control in sedentary community-dwelling older people. It can be a great resource for carrying out physical activities in this population., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
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6. High prevalence of end-stage renal disease of unknown origin in Aguascalientes Mexico: role of the registry of chronic kidney disease and renal biopsy in its approach and future directions.
- Author
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Gutierrez-Peña M, Zuñiga-Macias L, Marin-Garcia R, Ovalle-Robles I, García-Díaz AL, Macías-Guzmán MJ, Delgado-Bentites A, Macías-Diaz DM, Prado-Aguilar CA, Vega de la Rosa A, Delgadillo-Castañeda R, Chew-Won A, Reyes-Acevedo R, Reyes-Campos DM, Martínez-Guevara MA, Mendoza-Enciso EA, Nava-Becerra B, Piza-Jiménez MA, and Arreola Guerra JM
- Abstract
Background: Chronic kidney disease (CKD) is one of the pathologies with the greatest impact on the public health system. Over the last few decades, the relevance of CKD in Mexico has increased, with associated overwhelming costs for care of renal disease. There are no reliable CKD statistics in Mexico., Methodology: In June 2018, the government of Aguascalientes called on all Health Institutions to create a state registry of treated end-stage renal disease (ESRD). In the same system, a renal biopsy result registry included all the native kidney biopsies obtained in the state of Aguascalientes since 2012. We herein describe the prevalence, incidence and characteristics of the patients included in the CKD and renal biopsy registry in the state of Aguascalientes., Results: As of April 2020, the state has documented 2827 patients on renal replacement therapy (RRT), 1877 on dialysis and 950 that have been transplanted. The prevalence of patients on dialysis is 1326 per million population (p.m.p.), and if transplanted individuals are included, it is 1997 p.m.p. The incidence of treated ESRD in 2019 was 336 p.m.p. ( n = 474) in individuals with an average age of 45.6 years (standard deviation ±18), and in a higher proportion of men (61%). There is a bimodal distribution of the age at which RRT was initiated. The first and the most significant peaks are between the ages of 20 and 40 years and are usually the result of CKD of unknown cause (73%). The second peak is between 50 and 70 years of age, and CKD is usually the result of diabetes mellitus and systemic arterial hypertension (59.6%). Since January 2012, 423 biopsies have been recorded. The patient's ages were between 20 and 30 years ( n = 112), and the most frequent diagnosis was focal segmental glomerulosclerosis (FSGS) (54%)., Conclusions: The prevalence of treated ESRD in the state of Aguascalientes is high. The disease mostly afflicts young people between 20 and 40 years of age, and there is a clear male predominance. In this age group, the main clinical diagnosis is CKD of unknown origin, and the most frequent biopsy diagnosis was FSGS., (© The Author(s) 2021. Published by Oxford University Press on behalf of ERA-EDTA.)
- Published
- 2021
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7. The SPOT GRADE: A New Method for Reproducibly Quantifying Surgical Wound Bleeding.
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Spotnitz WD, Zielske D, Centis V, Hoffman R, Gillen DL, Wittmann C, Guyot V, Campos DM, Forest P, Pearson A, and McAfee PC
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- Humans, Prospective Studies, Reproducibility of Results, Severity of Illness Index, Surgeons, Blood Loss, Surgical, Hemorrhage diagnosis, Surgical Wound diagnosis
- Abstract
Study Design: Benchtop model with prospective surgeon video testing., Objective: To create a surface bleeding severity scale, the SPOT GRADE (SG), for quantitative assessment of target bleeding site (TBS) blood loss. This is of particular interest for spinal surgery due to epidural bleeding and an inability to use diathermy and radiofrequency cautery close to nerve roots., Summary of Background Data: A novel apparatus perfusable at known flow rates and simulating different sized wounds was used to create movies to educate surgeons on specific degrees of bleeding., Methods: Training (36) and testing (108) videos were created using a benchtop apparatus employing different bleeding severities based on the six-level SG (none, minimal, mild, moderate, severe, and extreme) and TBS sizes (1, 10, and 50 cm). Fourteen surgeons in four specialties (cardiothoracic, abdominal, spine, and orthopedic lower extremity) were trained and tested to evaluate SG characteristics including inter-rater and intrarater reliability., Results: The interclass correlation coefficient was estimated to be 0.89840 (95% confidence interval [CI]: 0.85771, 1), whereas the intraclass correlation coefficient was estimated to be 0.93673 (95% CI: 0.89603, 1). In 98% of cases (95% CI: 0.9736, 0.9927), surgeons correctly identified eligible bleeds for a future clinical trial (scores = 1, 2, or 3) and in 91% of cases (95% CI: 0.8895, 0.9344), surgeons correctly identified noneligible bleeds (scores = 4 or 5). In 98.6% of cases (95% CI: 0.9777, 0.9945), physicians correctly identified true hemostasis (score = 0). Based upon these data the probability of a physician rating a bleed incorrectly as hemostasis (score = 0) is estimated to be 1.51% (95% CI: 0.0061, 0.0363)., Conclusion: This SG is reproducible and reliable providing a basis for educating surgeons on TBS blood loss. It appears to be a new standard for evaluating wound blood loss., Level of Evidence: 2.
- Published
- 2018
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8. Conformation analysis of a novel fluorinated chalcone.
- Author
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Carvalho PS Jr, Custodio JM, Vaz WF, Cirilo CC, Cidade AF, Aquino GL, Campos DM, Cravo P, Coelho CJ, Oliveira SS, Camargo AJ, and Napolitano HB
- Abstract
Chalcones are an important class of natural compounds that exhibit numerous biological activities. In this paper, we report the synthesis and characterization of new fluorinated chalcone (FCH). The molecular geometry was determined by means of single crystal X-ray diffraction, and density functional theory (DFT) at B3LYP, M06-2X functionals and MP2 method, with the 6-311++G(d,p) basis set, was applied to optimize the ground state geometry and to study the molecular conformational stability. The molecular electrostatic potential (MEP) was also investigated at the same level of theory in order to identify and quantify the possible reactive sites. The FCH crystallizes in the centrossymmetric space group [Formula: see text] with two independent conformers (α and β) in the asymmetric unit cell. The α conformer is arranged in planar layer whereas the β creates a layer of non-classical dimer along c axis, that differ from α in about 11° in the orientation of phenyl groups. The stabilization of the β conformer is achieved by C-H···π arrangement. The small energy difference between the conformers (0.086 kcal mol
-1 ) and the absence of activation energy indicates that the conversion between them can takes place at room temperature and the β isomer is stable only in solid state. The FCH most electrophilic site occurs on the oxygen atom from the carboxyl group with absolute MEP value of about -52 kcal mol-1 whereas the MEP value calculated for F site is about -23 kcal mol-1 .- Published
- 2017
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9. A chitosan-hyaluronic acid hydrogel scaffold for periodontal tissue engineering.
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Miranda DG, Malmonge SM, Campos DM, Attik NG, Grosgogeat B, and Gritsch K
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- Animals, Cell Line, Tumor, Cell Survival, Humans, Materials Testing, Mice, NIH 3T3 Cells, Chitosan chemistry, Chitosan pharmacology, Hyaluronic Acid chemistry, Hyaluronic Acid pharmacology, Hydrogels chemistry, Hydrogels pharmacology, Periodontium metabolism, Tissue Engineering, Tissue Scaffolds chemistry
- Abstract
The current challenge in treating periodontitis is regenerating the periodontium. This motivates tissue-engineering researchers to develop scaffolds as artificial matrices that give mechanical support for osteoblasts, cementoblasts, gingival and periodontal ligament fibroblast cells. In this study, modified hyaluronic acid (HA) and chitosan (CS) were employed to create a hybrid CS-HA hydrogel scaffold for periodontal regeneration. CS, HA, and CS-HA scaffolds were obtained by freeze-drying technique, resulting in porous structures suitable for use in tissue engineering. Scaffolds were submitted to gamma and UV-sterilization without significant morphology changes. The ATR-FTIR spectra of CS-HA hydrogels showed peaks at 377 cm
-1 , 1566 cm-1 , and 1614 cm-1 , representing secondary amide, primary amine, and carboxyl acid respectively, and it was also observed the emergence of peaks at 886 cm-1 , which probably represents the Schiff base formed in the case of hybrid CS-HA hydrogels. The scaffolds presented a high rate of PBS uptake, reaching values higher than 95%. Thermal degradation of HA scaffolds was around 225°C and CS was around 285°C. The ATR-FTIR spectra and swelling degree were slightly disturbed mainly after gamma sterilization, but degradation temperature did not change after sterilization. The performance of the CS-HA hydrogel scaffolds for in vitro cell culture was tested using NIH3T3 and MG63 cell lines. The Alamar Blue test showed a significant increase in cellular viability and high CD44 expression, suggesting that the cells migrated more when seeded onto the scaffolds. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1691-1702, 2016., (© 2015 Wiley Periodicals, Inc.)- Published
- 2016
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10. Nursing interventions for rehabilitation in Parkinson's disease: cross mapping of terms.
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Tosin MH, Campos DM, Andrade LT, Oliveira BG, and Santana RF
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- Aged, Female, Humans, Male, Nursing Process, Parkinson Disease rehabilitation, Standardized Nursing Terminology
- Abstract
Objective: to perform a cross-term mapping of nursing language in the patient record with the Nursing Interventions Classification system, in rehabilitation patients with Parkinson's disease., Method: a documentary research study to perform cross mapping. A probabilistic, simple random sample composed of 67 records of patients with Parkinson's disease who participated in a rehabilitation program, between March of 2009 and April of 2013. The research was conducted in three stages, in which the nursing terms were mapped to natural language and crossed with the Nursing Interventions Classification., Results: a total of 1,077 standard interventions that, after crossing with the taxonomy and refinement performed by the experts, resulted in 32 interventions equivalent to the Nursing Interventions Classification (NIC) system. The NICs, "Education: The process of the disease.", "Contract with the patient", and "Facilitation of Learning" were present in 100% of the records. For these interventions, 40 activities were described, representing 13 activities by intervention., Conclusion: the cross mapping allowed for the identification of corresponding terms with the nursing interventions used every day in rehabilitation nursing, and compared them to the Nursing Interventions Classification.
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- 2016
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11. Performance and carbon turnover in fast- and slow-growing broilers submitted to cyclic heat stress and fed on high-protein diets.
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Campos DM, Macari M, Fernadez-Alarcon MF, Nogueira WC, de Souza FA, Hada FH, Lunedo R, and Denadai JC
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- Amino Acids administration & dosage, Animal Feed analysis, Animals, Chickens genetics, Chickens growth & development, Diet veterinary, Dietary Proteins administration & dosage, Dose-Response Relationship, Drug, Hot Temperature adverse effects, Liver drug effects, Male, Muscle, Skeletal drug effects, Pectoralis Muscles drug effects, Pectoralis Muscles metabolism, Random Allocation, Amino Acids metabolism, Chickens physiology, Dietary Proteins metabolism, Heat-Shock Response drug effects, Liver metabolism, Muscle, Skeletal metabolism
- Abstract
Two experiments were conducted to test the hypothesis that when using similar protein/amino acid diets and environment temperature conditions, the performance and carbon turnover in muscle and liver tissues, as measured by the incorporation of stable isotopes ((13)C/(12)C), must be different between fast-growing Cobb 500® and slow-growing Label Rouge broilers. For both experiments (Cobb and Label Rouge), 21-d-old birds were distributed in a completely randomised, 3 × 3 factorial design; three environmental temperatures (cyclic heat stress ad libitum, 22°C ad libitum, and 22°C restricted) and three crude protein concentrations (189.1, 210 and 220 g/kg CP) were used. The Cobb 500® had better performance with higher concentrations of crude protein. Cyclic heat stress (a temperature factor), negatively affected this genetic strain's performance. For the Label Rouge birds, the crude protein concentrations in the diet presented inconsistent results and cyclic heat stress did not affect the performance. The carbon turnover rate was affected in the Cobb 500® strain, with a high protein content reducing carbon turnover in the evaluated tissues (liver and muscles). Feed intake had a greater impact on carbon turnover rates than cyclic heat stress. The Label Rouge birds were not affected by the evaluated factors, suggesting that genetic improvement has a leading role on tissue carbon turnover. There is a genetic influence on carbon turnover in the liver and muscle tissues of broiler chickens. In addition, genetically fast-growing broilers are more susceptible to variations in diet composition and environmental temperature than less rapidly growing animals.
- Published
- 2016
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12. EVALUATION OF THE THERAPEUTIC EFFICACY OF LEVAMISOLE HYDROCHLORIDE ON THIRD-STAGE LARVAE OF Lagochilascaris minor IN EXPERIMENTALLY INFECTED MICE.
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Campos DM, Barbosa AP, Oliveira JA, Barbosa CA, Lobo TF, Silva LG, Thomaz DV, and Peixoto Jde C
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- Animals, Antinematodal Agents pharmacology, Ascaridida classification, Ascaridida Infections parasitology, Cats, Disease Models, Animal, Levamisole pharmacology, Mice, Mice, Inbred C57BL, Antinematodal Agents therapeutic use, Ascaridida drug effects, Ascaridida Infections drug therapy, Levamisole therapeutic use
- Abstract
Lagochilascariosis, a disease caused by Lagochilascaris minor, affects the neck, sinuses, tonsils, lungs, the sacral region, dental alveoli, eyeballs and the central nervous system of humans. A cycle of autoinfection may occur in human host tissues characterized by the presence of eggs, larvae and adult worms. This peculiarity of the cycle hinders therapy, since there are no drugs that exhibit ovicidal, larvicidal and vermicidal activity. Given these facts, we studied the action of levamisole hydrochloride on third-stage larvae in the migration phase (G1) and on encysted larvae (G3) of L. minor. To this end, 87 inbred mice of the C57BL/6 strain were divided into test groups comprising 67 animals (G1-37; G3-30) and a control group (G2-10; G4-10) with 20 animals. Each animal was inoculated orally with 2,000 infective eggs of the parasite. The animals of the test groups were treated individually with a single oral dose of levamisole hydrochloride at a concentration of 0.075 mg. The drug was administered either 30 minutes prior to the parasite inoculation (G1 animals) or 120 days after the inoculation (G3 animals). The mice in the control groups were not treated with the drug. After the time required for the migration and the encysting of L. minor larvae, all the animals were euthanized and their tissues examined. The data were analyzed using the Student's unpaired t-test and the Levene test. The groups showed no statistically significant difference. Levamisole hydrochloride was ineffective on third-stage larvae of L. minor. These findings explain the massive expulsion of live adult worms, as well as the use of long treatment schemes, owing to the persistence of larvae and eggs in human parasitic lesions.
- Published
- 2016
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13. [Mapping Nursing language terms of Parkinson's disease].
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Tosin MH, Campos DM, Blanco L, Santana RF, and Oliveira BG
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- Aged, Female, Humans, Male, Vocabulary, Controlled, Nursing Diagnosis, Parkinson Disease diagnosis, Parkinson Disease nursing, Terminology as Topic
- Abstract
Objective: Implementing cross-mapping of Nursing language terms with the terminology of NANDA International, contained in records of patients with Parkinson's disease in rehabilitation., Method: Descriptive study of cross mapping, carried out in three steps. A simple random sample of 67 files of patients who participated in the rehabilitation in the period between March 2009 and April 2013., Results: We identified 454 terms of Nursing language that resulted in 54 diagnoses after cross-mapping, present in 11 of the 13 taxonomy domains. The most mapped diagnosis was "Impaired urinary elimination" (59.7%), followed by "Urgent urinary incontinence" (55.2%), "Willingness to self-control improved health" (50.7%), "Constipation" (47.8%) and "Compromised physical mobility" (29.9%). Seven described terms were not mapped due to a corresponding defining characteristic being absent., Conclusion: It was possible to determine the profile of patients, as well as the complexity of nursing care in the rehabilitation of patients with Parkinson's disease.
- Published
- 2015
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14. Acidic pH resistance of grafted chitosan on dental implant.
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Campos DM, Toury B, D'Almeida M, Attik GN, Ferrand A, Renoud P, and Grosgogeat B
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- Cell Adhesion, Cells, Cultured, Chitosan pharmacology, Coated Materials, Biocompatible, Fibroblasts, Gingiva cytology, Humans, Hydrogen-Ion Concentration, Microscopy, Confocal, Silanes pharmacology, Spectroscopy, Fourier Transform Infrared, Surface Properties, Titanium pharmacology, Chitosan chemistry, Dental Implants, Silanes chemistry, Titanium chemistry
- Abstract
Over the last decade, access to dental care has increasingly become a service requested by the population, especially in the case of dental implants. However, the major cause of implant failure is an inflammatory disease: peri-implantitis. Currently, the adhesion strength of antibacterial coatings at implant surfaces remains a problem to solve. In order to propose a functionalized implant with a resistant antibacterial coating, a novel method of chitosan immobilization at implant surface has been investigated. Functionalization of the pre-active titanium (Ti) surface was performed using triethoxysilylpropyl succinic anhydride (TESPSA) as a coupling agent which forms a stable double peptide bond with chitosan. The chitosan presence and the chemical resistibility of the coating under acid pH solutions (pH 5 and pH 3) were confirmed by FTIR-ATR and XPS analyses. Furthermore, peel test results showed high adhesive resistance of the TESPSA/chitosan coating at the substrate. Cytocompatibility was evaluated by cell morphology with confocal imaging. Images showed healthy morphology of human gingival fibroblasts (HGF-1). Finally, the reported method for chitosan immobilization on Ti surface via peptide bindings allows for the improvement of its adhesive capacities and resistibility while maintaining its cytocompatibility. Surface functionalization using the TESPSA/chitosan coupling method is noncytotoxic and stable even in drastic environments as found in oral cavity, thus making it a valuable candidate for clinical implantology applications.
- Published
- 2015
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15. Photoprotection of Buddleja cordata extract against UVB-induced skin damage in SKH-1 hairless mice.
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Avila Acevedo JG, Espinosa González AM, De Maria y Campos DM, Benitez Flores Jdel C, Hernández Delgado T, Flores Maya S, Campos Contreras J, Muñoz López JL, and García Bores AM
- Subjects
- Administration, Topical, Animals, Erythema prevention & control, Female, Mice, Mice, Hairless, Phenols chemistry, Phenols pharmacology, Plant Extracts chemistry, Random Allocation, Skin pathology, Sunburn prevention & control, Sunscreening Agents chemistry, Buddleja chemistry, Plant Extracts pharmacology, Skin drug effects, Skin radiation effects, Sunscreening Agents pharmacology, Ultraviolet Rays adverse effects
- Abstract
Background: In recent years, there has been considerable interest in using botanical agents to prevent skin damage resulting from solar UV-irradiation. Buddleja cordata is a plant that is known as "tepozan". Some people in Mexico use the leaves of this plant to treat tumours, abscesses, sores and burns. The purpose of this study is to investigate the photoprotective properties of Buddleja cordata methanolic extract (BCME) against UVB-induced skin damage in SKH-1 hairless mice at the macroscopic and histological levels., Methods: BCME was characterised to determine its spectroscopic, chromatographic and antioxidant (DPPH, superoxide and hydroxyl radicals) properties. To conduct the photoprotection studies, BCME was applied topically to the skin of SKH-1 mice before acute exposure to UVB for 10 minutes. The murine skin samples were used for macroscopic and histological studies to assess tissue damage. Penetration of active components of BCME into stratum corneum on the dorsal area of mice was investigated in vivo by the tape stripping method. Moreover, genotoxicity of BCME was evaluated in a Vicia faba cell root micronucleus model., Results: BCME displayed absorbance over the entire UVB spectrum, and its principal components included verbascoside and linarin. BCME exhibited antioxidant activity and significantly scavenged hydroxyl radicals. BCME reduced erythema, sunburn cell production, vessel congestion and epidermal thickening of UVB irradiated mouse skin. BCME penetrate the skin of mice. BCME did not exhibit genotoxic activity in the micronucleus test., Conclusion: The topical administration of BCME protected against acute UVB-induced damage in mouse SKH-1 skin, and our results suggest that BCME may potentially prevent photodamage.
- Published
- 2014
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16. Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.
- Author
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Campos DM, Gritsch K, Salles V, Attik GN, and Grosgogeat B
- Abstract
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.
- Published
- 2014
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17. Effects of corn replacement by sorghum in broiler diets on performance and intestinal mucosa integrity.
- Author
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Torres KA, Pizauro JM Jr, Soares CP, Silva TG, Nogueira WC, Campos DM, Furlan RL, and Macari M
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- Alkaline Phosphatase genetics, Alkaline Phosphatase metabolism, Aminopeptidases genetics, Aminopeptidases metabolism, Animal Nutritional Physiological Phenomena, Animals, Cell Proliferation, Intestinal Mucosa cytology, Male, Weight Gain, Animal Feed analysis, Chickens growth & development, Diet veterinary, Intestinal Mucosa physiology, Sorghum, Zea mays
- Abstract
The effect of replacing corn with low-tannin sorghum on broiler performance, carcass yield, integrity of mucosa of small intestine segments, and activity of membrane enzymes of the jejunum is investigated. A total of 594 male Cobb-500 broiler chicks were randomly assigned to 3 dietary treatments: 100% corn (control), 50% corn replacement with low-tannin sorghum (low sorghum), and 100% corn replacement with low-tannin sorghum (high sorghum). Body weight gain, feed consumption, feed conversion, and carcass yield were determined at 7, 21, and 42 d, and segments of the small intestine were collected. Feed conversion and weight gain were impaired at d 42 in broilers fed the high-sorghum diet, but no differences were observed for carcass yield among the treatments (P > 0.05). Crypt cell mitotic index of the jejunum and ileum at d 21 and 42 was lower in broilers fed the control diet than in those fed low- and high-sorghum diets (P < 0.05). Aminopeptidase activity was higher in broilers fed the control diet than in those fed low- and high-sorghum diets irrespective of age (P < 0.05). Conversely, intestinal alkaline phosphatase activity in the small intestine did not differ among the dietary treatments (P > 0.05). Our results indicate that 50% corn replacement with low-tannin sorghum is suitable for broiler diets, whereas 100% corn replacement with low-tannin sorghum had negative effects on the intestinal mucosa and performance of broilers at 42 d.
- Published
- 2013
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18. Role of culture conditions on in vitro transformation and cellular colonization of biomimetic HA-Col scaffolds.
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Campos DM, Soares GA, and Anselme K
- Subjects
- Biomimetic Materials, Cell Differentiation, Cell Survival, Cells, Cultured, Culture Media, Humans, In Vitro Techniques, Collagen chemistry, Hyaluronic Acid chemistry, Tissue Engineering methods, Tissue Scaffolds chemistry
- Abstract
We have recently developed new 3D hydroxyapatite/collagen (50/50 wt%) scaffolds using a biomimetic synthesis approach. The first in vitro tests performed in static culture showed a limited cell colonization and survival inside the scaffolds. The current study evaluated in dynamic culture the scaffold changes and colonization by human immortalized osteoprogenitor STRO-1A cells. The stability of our scaffolds in the different culture conditions (static, low flow, high flow) was validated by the maintenance of the pore diameter and interconnectivity over 21 d. The colonization and the viability of STRO-1A cells inside the scaffolds were further evaluated on histological sections. It was demonstrated that only the high flow-rate allowed cell survival after 7 d and a complete scaffold colonization. Moreover, the colonization and viability were different in function of the scaffold position inside the perfusion container. The differentiation markers (alkaline phosphatase activity, type I procollagen and osteocalcin synthesis) of STRO-1A cells were analyzed in the culture medium after 7, 14 and 21 d. The low flow-rate increased significantly the three markers compared with static conditions. In contrast, markers were reduced in high flow-rate compared with low flow-rate. To explain this surprising result, we hypothesized that the different molecules were actually adsorbed on the scaffold because of the closed circuit used in the high flow-rate conditions. In summary, this study provides original results on the influence of flow rate but mostly of the circuit used (open/closed) on the structural modifications and cell colonization of collagen-HA scaffolds.
- Published
- 2013
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19. Increasing information-seeking about human papillomavirus vaccination through community partnerships in African American and Hispanic communities.
- Author
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Kreuter MW, Fernandez ME, Brown M, Cofta-Woerpel L, Pfeiffer D, Adams-Piphus B, Krebill H, Gonzalez DA, Campos DM, Kirklin GT, Betsworth S, Casey C, and Luke D
- Subjects
- Adult, Black or African American psychology, Community Participation statistics & numerical data, Community-Institutional Relations, Feasibility Studies, Female, Health Services Accessibility, Healthcare Disparities, Hispanic or Latino psychology, Humans, Information Services, Kansas, Missouri, Parents, Patient Acceptance of Health Care, Telephone, Texas, Young Adult, Black or African American education, Health Knowledge, Attitudes, Practice, Hispanic or Latino education, Information Seeking Behavior, Papillomavirus Infections prevention & control, Papillomavirus Vaccines
- Abstract
This study tested the feasibility of promoting 1-800-4-CANCER through partnerships with organizations serving African American and Hispanic communities. Small-media and client reminders about human papillomavirus vaccination were made available through local agents to 28 community organizations. Organizations ordered 79 932 resources and distributed them to young women and parents of girls-;African Americans in St Louis, Missouri, and Hispanics in the Lower Rio Grande Valley of Texas. Pre- to postintervention calls to 1-800-4-CANCER increased 38% in these communities, while declining 15% in comparison communities of Kansas City, Missouri, and El Paso, Texas (F = 8.6, P = .004) and 1.4% in the United States as a whole.
- Published
- 2012
- Full Text
- View/download PDF
20. IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by ELISA using Strongyloides ratti saline extract as heterologous antigen.
- Author
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Rodrigues RM, de Oliveira MC, Sopelete MC, Silva DA, Campos DM, Taketomi EA, and Costa-Cruz JM
- Subjects
- Animals, Antigens, Helminth isolation & purification, Feces parasitology, Humans, Larva, Strongyloides ratti chemistry, Strongyloides ratti immunology, Strongyloides stercoralis isolation & purification, Strongyloidiasis immunology, Antibodies, Helminth blood, Antigens, Heterophile isolation & purification, Enzyme-Linked Immunosorbent Assay methods, Immunoglobulin E blood, Immunoglobulin G blood, Strongyloidiasis diagnosis
- Abstract
The aim of this study was to evaluate total IgG, IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by enzyme-linked immunosorbent assay (ELISA) using Strongyloides ratti saline extract as heterologous antigen for a possible clinical utility of the assay. A total of 40 serum samples of patients who were shedding Strongyloides stercoralis larvae in feces (group I), 30 sera from patients with other intestinal parasites (group II), and 30 sera from subjects with negative results in three parasitological assays (group III) were analyzed to detect total IgG, IgG1, IgG4, and IgE to Strongyloides spp. by ELISA and expressed in ELISA index. Levels of total IgG anti-Strongyloides spp. were significantly higher in patients of group I than in groups II (p=0.0005) and III (p<0.0001). Levels of specific IgG1, IgG4, and IgE of group I were also significantly higher than in groups II and III, respectively. There was a significant positive correlation between specific IgE and IgG4 (r=0.6524; p=0.0084) and IgG1 and IgG4 (r=0.5398; p=0.0171). It can be concluded that the detection of specific IgE, IgG1, and IgG4 subclasses rather than total IgG antibodies to Strongyloides spp. using the S. ratti antigen showed to be an additional tool for improving the serodiagnosis of human strongyloidiasis.
- Published
- 2007
- Full Text
- View/download PDF
21. Lagochilascaris minor third-stage larvae secrete metalloproteases with specificity for fibrinogen and native collagen.
- Author
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Barbosa AP, Campos DM, Semerene AR, Teixeira AR, and Santana JM
- Subjects
- Animals, Ascaridida Infections parasitology, Ascaridida Infections pathology, Ascaridoidea growth & development, Electrophoresis, Polyacrylamide Gel, Enzyme Stability, Gelatin metabolism, Histocytochemistry, Hydrogen-Ion Concentration, Larva enzymology, Mesentery pathology, Metalloproteases biosynthesis, Metalloproteases isolation & purification, Mice, Mice, Inbred C57BL, Molecular Weight, Serum Albumin, Bovine metabolism, Ascaridoidea enzymology, Collagen metabolism, Fibrinogen metabolism, Metalloproteases metabolism
- Abstract
Dissemination of parasitic infections depends on migration through tissues and evasion from both hemostatic processes and immune responses from hosts. Metalloproteases play major roles in these mechanisms of pathogen-host interactions and, thus, are considered drug targets. In this study, we characterized metalloprotease activities in excretory/secretory (ES) products from third stage larvae (L3) of the ascarid Lagochilascaris minor, the causative agent of lagochilascariosis, which demonstrates an impressive migrating capacity across host tissues, including bone. Gel enzymography showed that ES products of L3 display two major gelatinolytic activities. Optimal proteolytic activity was found to occur at neutral/alkaline pH and was associated with two L. minor-secreted metalloproteases of 59 (SM59(Lm)) and 114kDa (SM114(Lm)). We next showed that ES products of L3 were able to hydrolyze fibrinogen and collagen I at neutral pH, but not BSA, in an extensive manner. Furthermore, we demonstrated that ES products of L3 mediate hydrolysis of the triple helical structure of collagen I fibers in mouse mesentery. These results suggest that ES proteases of L3 might facilitate both L. minor migration through host tissues by hydrolyzing collagens of the extracellular matrix and evasion from host hemostatic mechanisms by degrading fibrinogen.
- Published
- 2006
- Full Text
- View/download PDF
22. Novel down-regulatory mechanism of the surface expression of the vasopressin V2 receptor by an alternative splice receptor variant.
- Author
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Sarmiento JM, Añazco CC, Campos DM, Prado GN, Navarro J, and González CB
- Subjects
- Amino Acid Sequence, Animals, COS Cells, Cell Membrane metabolism, Cricetinae, DNA, Complementary metabolism, Dimerization, Dogs, Dose-Response Relationship, Drug, Fluorescence Resonance Energy Transfer, Genes, Dominant, Immunohistochemistry, Immunoprecipitation, Interleukin-8 metabolism, Kidney metabolism, Microscopy, Confocal, Molecular Sequence Data, Protein Binding, Protein Structure, Tertiary, RNA, Messenger metabolism, Rats, Sequence Homology, Amino Acid, Subcellular Fractions metabolism, Transfection, Alternative Splicing, Down-Regulation, Receptors, Vasopressin chemistry, Receptors, Vasopressin genetics
- Abstract
In rat kidney, two alternatively spliced transcripts are generated from the V2 vasopressin receptor gene. The large transcript (1.2 kb) encodes the canonical V2 receptor, whereas the small transcript encodes a splice variant displaying a distinct sequence corresponding to the putative seventh transmembrane domain and the intracellular C terminus of the V2 receptor. This work showed that the small spliced transcript is translated in the rat kidney collecting tubules. However, the protein encoded by the small transcript (here called the V2b splice variant) is retained inside the cell, in contrast to the preferential surface distribution of the V2 receptor (here called the V2a receptor). Cells expressing the V2b splice variant do not exhibit binding to 3H-labeled vasopressin. Interestingly, we found that expression of the splice variant V2b down-regulates the surface expression of the V2a receptor, most likely via the formation of V2a.V2b heterodimers as demonstrated by co-immunoprecipitation and fluorescence resonance energy transfer experiments between the V2a receptor and the V2b splice variant. The V2b splice variant would then be acting as a dominant negative. The effect of the V2b splice variant is specific, as it does not affect the surface expression of the G protein-coupled interleukin-8 receptor (CXCR1). Furthermore, the sequence encompassing residues 242-339, corresponding to the C-terminal domain of the V2b splice variant, also down-regulates the surface expression of the V2a receptor. We suggest that some forms of nephrogenic diabetes insipidus are due to overexpression of the splice variant V2b, which could retain the wild-type V2a receptor inside the cell via the formation of V2a.V2b heterodimers.
- Published
- 2004
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23. Experimental lagochilascariosis: histopathological study of inflammatory response to larval migration in the Murine model.
- Author
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Semerene AR, Lino Rde S Jr, Oliveira JA, Magalhães AV, Stefani MM, Barbosa AP, and Campos DM
- Subjects
- Adult, Animals, Child, Disease Models, Animal, Female, Humans, Larva growth & development, Larva pathogenicity, Male, Mice, Mice, Inbred C57BL, Nematoda pathogenicity, Time Factors, Nematoda growth & development, Nematode Infections parasitology
- Abstract
The goal of this study was to investigate the pattern of inflammatory response induced by Lagochilascaris minor in murine experimental model. For this purpose 115 mice were given 1000-3000 L. minor infective eggs "per os" and 51 uninfected mice were considered as controls. Four hours post-inoculation (PI), 3rd stage larvae were seen passing through the mucosa of terminal ends of small intestine. Six hours PI larvae were observed as an embolus inside the portal vein and also migrating through the liver parenchyma. During the first 24 h larvae-containing eggs of L. minor were observed in the lumen of intestinal tract. Two days PI larvae were seen migrating through lung parenchyma associated with an initial neutrophilic perivasculitis. From the 13th day of this experimental study, L. minor larvae were found mainly in skeletal muscles, in the center of granulomas. Concentric fibrosis with mixed inflammatory infiltrate involved the larvae after the 47th day PI, persistently. This experimental murine study with L. minor indicated that the 3rd stage larvae penetrated via ileum-cecal mucosa reaching the liver and probably other tissues through the hematogenic via. Throughout its pathway the larvae induced a granulomatous reaction, with abundant polimorphonuclear cells.
- Published
- 2004
- Full Text
- View/download PDF
24. Heterologous antigen extract in ELISA for the detection of human IgE anti-Strongyloides stercoralis.
- Author
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Costa-Cruz JM, Madalena J, Silva DA, Sopelete MC, Campos DM, and Taketomi EA
- Subjects
- Animals, Antigens, Helminth isolation & purification, Antigens, Heterophile isolation & purification, Biomarkers blood, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Sensitivity and Specificity, Antibodies, Helminth blood, Antigens, Helminth immunology, Antigens, Heterophile immunology, Immunoglobulin E blood, Strongyloides ratti immunology, Strongyloidiasis immunology
- Abstract
Strongyloides ratti larval extract was used for the standardization of ELISA to detect genus-specific IgE in human strongyloidiasis. Forty serum samples from monoinfected patients shedding S. stercoralis larvae (Group I), 40 from patients with other intestinal parasites (Group II), and 40 from copronegative healthy subjects (Group III) were analyzed. Genus-specific IgE levels (ELISA Index: EI) were significantly higher in the group I (EI = 1.43) than groups II (EI = 0.70) and III (EI = 0.71), showing positivity rates of 55%, 2.5% and 0%, respectively. Similarly, sera from copropositive patients had significantly higher levels of total IgE (866 IU/mL) as compared to those from group II (302 IU/mL) and III (143 IU/mL). A significant positive correlation was found between levels of Strongyloides specific-IgE and total IgE in sera from patients with strongyloidiasis. In conclusion, S. ratti heterologous extract showed to be a useful tool for detecting genus-specific IgE by ELISA, contributing for a better characterization of the immune response profile in human strongyloidiasis.
- Published
- 2003
- Full Text
- View/download PDF
25. Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis.
- Author
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Silva LP, Barcelos IS, Passos-Lima AB, Espindola FS, Campos DM, and Costa-Cruz JM
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Biomarkers blood, Blotting, Western, Case-Control Studies, Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay, Female, Fluorescent Antibody Technique, Indirect, Humans, Immunoglobulin G immunology, Male, Middle Aged, Rats, Sensitivity and Specificity, Strongyloidiasis immunology, Antibodies, Helminth blood, Antigens, Helminth immunology, Immunoglobulin G blood, Strongyloides ratti immunology, Strongyloidiasis diagnosis
- Abstract
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5% of the cases of strongyloidiasis. The negative concordance in the three tests was 94% and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology.
- Published
- 2003
- Full Text
- View/download PDF
26. Human bertiellosis in Goiás, Brazil: a case report on human infection by Bertiella sp. (Cestoda: Anoplocephalidae).
- Author
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Paçô JM, Campos DM, and Araújo JL
- Subjects
- Animals, Cestode Infections diagnosis, Child, Preschool, Feces parasitology, Female, Humans, Parasite Egg Count, Cestode Infections parasitology
- Abstract
The authors report on a new case of human Bertiellosis in a 2-year old female patient who was born in Goiânia-Goiás (Brazil) and has had history of permanent dwelling in an area frequently visited by simians in Mato Grosso (Brazil). At the time of diagnosis the patient showed inappetence, abdominal pain, and loss of weight. Eggs and proglottids were found in her stool and were identified as Bertiella sp. The objective of this report is to register the third case of human Bertiellosis in Brazil, characterizing one more case of helminthic zoonosis.
- Published
- 2003
- Full Text
- View/download PDF
27. [A case of parasitism by Rhabditis sp in a child from Goiânia, Goiás, Brazil].
- Author
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Campos DM, Araújo JL, Vieira MC, Damasceno F, and Barbosa AP
- Subjects
- Animals, Antinematodal Agents therapeutic use, Diagnosis, Differential, Female, Humans, Infant, Male, Rhabditida Infections drug therapy, Rhabditoidea anatomy & histology, Thiabendazole therapeutic use, Feces parasitology, Rhabditida Infections diagnosis, Rhabditoidea isolation & purification
- Abstract
We report a case of parasitism by Rhabditis sp in a five-month-old child, from Goiás State, Brazil. He had a history of diarrhea with liquid and green faeces which later became bloody. Parasitological examination revealed the presence of larvae and adult female of Rhabditis sp. We used thiabendazole in this case after which the child improved. The authors suggest a better differential diagnostic between Strongyloides and Rhabditis.
- Published
- 2002
28. Polarized expression of the GFP-tagged rat V(1a) vasopressin receptor.
- Author
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Campos DM, Reyes CE, Sarmiento J, Navarro J, and González CB
- Subjects
- Animals, Calcium metabolism, Cell Line, DNA, Complementary metabolism, Dogs, Dose-Response Relationship, Drug, Genetic Vectors, Green Fluorescent Proteins, Kinetics, Liver metabolism, Microscopy, Confocal, Protein Binding, Rats, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Transfection, Luminescent Proteins metabolism, Receptors, Vasopressin biosynthesis, Receptors, Vasopressin chemistry, Recombinant Fusion Proteins metabolism
- Abstract
We investigated the targeting of the V(1a) receptor fused with the green fluorescence protein (V(1a)R-GFP) in polarized MDCK cells. Cells expressing V(1a)R-GFP displayed binding to vasopressin (AVP) and AVP-induced calcium responses, similar to cells expressing the wild-type V1a receptor. Interestingly, as with the wild-type V(1a)R, V(1a)R-GFP is preferentially distributed in the basolateral side of MDCK cells as monitored by confocal microscopy. Furthermore, AVP induced internalization of GFP-tagged receptors. Therefore, the GFP-tagged V(1a) receptor retains all the sorting signals of the wild-type receptor and offers an excellent system to elucidate the mechanisms of cell trafficking of V(1a) receptors.
- Published
- 2001
- Full Text
- View/download PDF
29. [Assessment of ivermectin therapeutic efficacy on fourth-stage larvae of Lagochilascaris minor in experimentally infected cats].
- Author
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Barbosa CA and Campos DM
- Subjects
- Animals, Cats, Larva drug effects, Larva growth & development, Nematode Infections drug therapy, Antinematodal Agents therapeutic use, Cat Diseases drug therapy, Ivermectin therapeutic use, Nematoda drug effects, Nematoda growth & development, Nematode Infections veterinary
- Abstract
In order to verify the action of ivermectin against fourth-stage larvae of Lagochilascaris minor, thirty cats were divided into three groups (I, II and III). Each animal was inoculated orally with 50 third-stage larvae. The cats from groups I and II were treated with Ivermectin (200/microg/kg/single dose/sc via) on fifth day after inoculation (DAI). Treatment evaluation was performed between 30 and 40 DAI (group I) and between 180 to 190 DAI (group II) using parasite macroscopic and microscopic research at autopsy. The 10 cats from group III were untreated (control group). The authors observed 100% drug efficacy, at all observation periods, by total interruption of parasite's biological cycle in each of the treated animals. All the control group developed Lagochilascaris minor infection.
- Published
- 2001
- Full Text
- View/download PDF
30. Parasitological and immunological diagnoses of strongyloidiasis in immunocompromised and non-immunocompromised children at Uberlândia, State of Minas Gerais, Brazil.
- Author
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de Paula FM, de Castro E, Gonçalves-Pires Md, Marçal Md, Campos DM, and Costa-Cruz JM
- Subjects
- Animals, Brazil, Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay, Feces parasitology, Fluorescent Antibody Technique, Indirect, Humans, Immunoglobulin G blood, Immunoglobulin M blood, Infant, Infant, Newborn, Strongyloides stercoralis immunology, Strongyloides stercoralis parasitology, Strongyloidiasis blood, Antibodies, Helminth blood, Immunocompromised Host, Strongyloides stercoralis isolation & purification, Strongyloidiasis diagnosis
- Abstract
Parasitological and immunological diagnoses were part of a study conducted among 151 children, 83 immunocompromised (IC) and 68 non-immunocompromised (non-IC) aged from zero to 12, seen at the University Hospital, Universidade Federal de Uberlândia, State of Minas Gerais, Brazil, from February, 1996, to June, 1998. Three fecal samples from each child were analyzed for the parasitological diagnosis by Baermann-Moraes and Lutz methods. The immunological diagnosis to detect IgG and IgM antibodies was carried out by the indirect immunofluorescence antibody test (IFAT) with cryo-microtome sections of Strongyloides stercoralis and Strongyloides ratti larvae as antigens and by the ELISA test with an alkaline extract of S. ratti as the antigens. Of the 151 children 5 (3.31%) were infected with larvae of S. stercoralis (2 cases IC, 2.41%, and 3 cases non-IC, 4.41%). The IFAT-IgG detected 7 (8.43%) serum samples positive among IC, and 2 (2.94%) cases among non-IC. The ELISA-IgG test detected 10 (12.05%) serum samples positive among IC, and 1 (1.47%) case among non-IC. The IFAT-IgM detected 6 (7.22%) positive cases among IC, and 3 (4.41%) cases among non-IC. ELISA-IgM test detected 10 (12.05%) positive cases among IC, and 3 (4.41%) cases among non-IC. It was concluded that the immunological tests can help in the diagnosis of strongyloidiasis in immunocompromised children.
- Published
- 2000
- Full Text
- View/download PDF
31. Wild rodents as experimental intermediate hosts of Lagochilascaris minor Leiper, 1909.
- Author
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Paçô JM, Campos DM, and Oliveira JA
- Subjects
- Animals, Cats, Female, Host-Parasite Interactions, Male, Nematoda growth & development, Nematoda isolation & purification, Animals, Wild parasitology, Disease Models, Animal, Nematoda physiology, Nematode Infections parasitology, Rodentia parasitology
- Abstract
A total of 25 specimens of Cavia porcellus (guinea pig), 5 Dasyprocta agouti (agouti), and 22 Calomys callosus (vesper mice) were inoculated with infective eggs of Lagochilascaris minor. The inoculum was prepared with embryonated eggs and orally administered to each individual animal through an esophagus probe. In parallel, 100 specimens of Felis catus domesticus were individually fed with 55-70 nodules containing 3rd-stage larvae encysted in tissues of infected rodents. Animals were examined and necropsied at different time intervals. The migration and encystment of L3 larva was observed in viscera, skeletal muscle, adipose and subcutaneous tissues from all rodents. Adult worms localized at abscesses in the cervical region, rhino, and oropharynx were recovered from domestic cats inoculated with infected rodent tissues. Through this study we can conclude that: (1) wild rodents act as intermediate hosts, characterizing this ascarid heteroxenic cycle; (2) in natural conditions rodents could possibly act as either intermediate hosts or paratenic hosts of Lagochilascaris minor; (3) despite the occurrence of an auto-infecting cycle, in prime-infection of felines (definite hosts) the cycle is only completed when intermediate hosts are provided; and (4) in the wild, rodents could serve as a source of infection for humans as they are frequently used as food in regions with the highest incidence of human lagochilascariasis.
- Published
- 1999
- Full Text
- View/download PDF
32. Seroepidemiological study of human strongyloidiasis with blood samples collected on filter paper, in Abadia dos Dourados (Minas Gerais, Brazil).
- Author
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Costa-Cruz JM, Machado ER, and Campos DM
- Subjects
- Adult, Aged, Animals, Brazil epidemiology, Child, Child, Preschool, Female, Humans, Male, Middle Aged, Rural Population, Seroepidemiologic Studies, Sex Distribution, Strongyloidiasis blood, Antibodies, Helminth blood, Strongyloides immunology, Strongyloidiasis epidemiology
- Published
- 1998
- Full Text
- View/download PDF
33. Assessment of ivermectin therapeutic efficacy on third-stage larvae of Lagochilascaris minor in mice experimentally infected.
- Author
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Barbosa CA, Campos DM, and de Oliveira JA
- Subjects
- Animals, Antinematodal Agents pharmacology, Cats, Dose-Response Relationship, Drug, Female, Ivermectin pharmacology, Larva drug effects, Male, Mice, Mice, Inbred C57BL, Nematoda drug effects, Antinematodal Agents therapeutic use, Ivermectin therapeutic use, Nematode Infections drug therapy
- Abstract
In this study we evaluated the potential action of ivermectin on third-stage larvae, both at migratory and encysted phases, in mouse tissues after experimental infection with Lagochilascaris minor. Study groups I and II consisted of 120 mice that were orally administered 1,000 parasite eggs. In order to assess ivermectin action upon migratory larvae, group I (60 mice) was equally split in three subgroups, namely I-A, I-B, and I-C. On the 7th day after inoculation (DAI), each animal from the subgroup I-A was treated with 200 micrograms/Kg ivermectin while subgroup I-B was given 1,000 micrograms/Kg, both groups received a single subcutaneous dose. To assess the drug action on encysted larvae, group II was equally split in three subgroups, namely II-A, II-B, II-C. On the 45th DAI each animal was treated with ivermectin at 200 micrograms/Kg (subgroup II-A) and 1,000 micrograms/Kg (group II-B) with a single subcutaneous dose. Untreated animals of subgroups I-C and II-C were used as controls. On the 60th DAI all animals were submitted to larva search. At a dose of 1,000 micrograms/Kg the drug had 99.5% effectiveness on third-stage migratory larvae (subgroup I-B). Ivermectin efficacy was lower than 5% on third-stage encysted larvae for both doses as well as for migratory larvae treated with 200 micrograms/Kg.
- Published
- 1998
- Full Text
- View/download PDF
34. Cryo-microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis.
- Author
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Costa-Cruz JM, Bullamah CB, Gonçalves-Pires Mdo R, Campos DM, and Vieira MA
- Subjects
- Animals, Cryoultramicrotomy, Feces parasitology, Fluorescent Antibody Technique, Indirect, Humans, Larva immunology, Rats, Sensitivity and Specificity, Antigens, Helminth immunology, Strongyloides ratti immunology, Strongyloides stercoralis immunology, Strongyloidiasis diagnosis
- Abstract
Cryo-microtome sections of larvae of Strongyloides stercoralis and S. ratti respectively obtained from human and rat feces cultures, were used as antigens. Fluoresceinate conjugates against human IgG were employed at the ideal titer of 10 for S. stercoralis and 100 for S. ratti. The sensitivity of the indirect immunofluorescence reaction (IIF) was 94.4% and 92.5% and the specificity 94.2% and 97.1% for the two specific larval antigens, respectively. Sera from 123 persons (54 from carriers of S. stercoralis infections and 69 from controls) were submitted to the reaction. The titers of different sera varied from 20 to 2560. There was a significant linear correlation (r = 0.85 p < or = 0.001) between the antibodies from the two species of larval antigens. We conclude that both antigens may be used in the IIF reaction for the diagnosis of human strongyloidiasis. Due to the feasibility of safe and low-cost mass production of S. ratti larvae in the laboratory with a considerable economy of conjugate, their utilization in the serum diagnosis of human strongyloidiasis is recommended.
- Published
- 1997
- Full Text
- View/download PDF
35. [Subependymal nodular heterotopia: morphological variability].
- Author
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Campos DM, Alarcón Alacio J, and Ruiz Ezquerro JJ
- Subjects
- Choristoma complications, Choristoma diagnosis, Electroencephalography, Epilepsy, Tonic-Clonic etiology, Humans, Magnetic Resonance Imaging, Male, Middle Aged, Brain physiopathology, Choristoma physiopathology
- Published
- 1996
36. Acquired bilateral opercular lesions or Foix-Chavany-Marie syndrome and eating epilepsy.
- Author
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Mateos V, Salas-Puig J, Campos DM, Carrero V, and Andermann F
- Subjects
- Adult, Epilepsy diagnosis, Facial Paralysis diagnosis, Facial Paralysis physiopathology, Functional Laterality, Humans, Magnetic Resonance Imaging, Male, Paralysis diagnosis, Pharyngeal Muscles pathology, Pharyngeal Muscles physiopathology, Syndrome, Epilepsy physiopathology, Paralysis physiopathology
- Published
- 1995
- Full Text
- View/download PDF
37. [Recurrent confusional states and periodic lateralized epileptiform discharges: a new type of non-convulsive status epilepticus?].
- Author
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Mateos V, Salas-Puig J, Campos DM, Tuñón A, Roiz C, and Lahoz CH
- Subjects
- Aged, Confusion etiology, Confusion physiopathology, Electroencephalography, Epilepsy complications, Epilepsy physiopathology, Female, Humans, Male, Parietal Lobe diagnostic imaging, Recurrence, Temporal Lobe diagnostic imaging, Tomography, Emission-Computed, Single-Photon, Confusion diagnosis, Epilepsy diagnosis, Parietal Lobe physiopathology, Temporal Lobe physiopathology
- Abstract
Periodic lateralized epileptiform discharges (PLEDs) in an EEG usually indicate the presence of an underlying structural lesion (of vascular origin in most cases). PLEDs are also sometimes observed in certain types of infections (mainly viral), in which they may even constitute a characteristic finding useful for diagnostic purposes. In recent years cases have been reported in which PLEDs are linked to recurring confusional states that do not fit in with established classifications and that may be epileptic in nature. We discuss the cases of 2 patients who were repeatedly admitted to our hospital in confusional states, in whom PLEDs were observed in EEG readings. Clinical evolution in both cases paralleled EEG alterations. We were able to perform both critical and intercritical single proton emission tomography on 1 patient, finding, respectively, hyper- and hypoabsorption foci. Symptoms resolved with antiepileptic treatment.
- Published
- 1995
38. [Wernicke's encephalopathy in non-alcoholic patients: a diagnosis to be considered].
- Author
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Mateos V, Campos DM, Salas-Puig J, and Fernández JM
- Subjects
- Adult, Female, Humans, Wernicke Encephalopathy diagnosis
- Published
- 1994
39. [Parkinsonism induced by metoclopramide].
- Author
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Mateos V, Caminal L, Colosía VP, and Campos DM
- Subjects
- Female, Humans, Middle Aged, Metoclopramide adverse effects, Parkinson Disease etiology
- Published
- 1993
40. Experimental life cycle of Lagochilascaris minor Leiper, 1909.
- Author
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Campos DM, Freire Filha LG, Vieira MA, Paçô JM, and Maia MA
- Subjects
- Animals, Cats, Cricetinae, Dogs, Female, Host-Parasite Interactions, Larva growth & development, Larva pathogenicity, Male, Mice, Mice, Inbred C57BL, Nematoda pathogenicity, Time Factors, Disease Models, Animal, Nematoda growth & development, Nematode Infections parasitology
- Abstract
The life cycle of Lagochilascaris minor was studied using material collected from human lesion and applying the experimental model: rodents (mice, hamsters), and carnivores (cats, dogs). In mice given infective eggs, orally, hatch of the third stage larvae was noted in the gut wall, with migration to liver, lungs, skeletal musculature and subcutaneous tissue becoming, soon after, encysted. In cats infected with skinned carcasses of mice (60 to 235 days of infection) it was observed: hatch of third stage larvae from the nodules (cysts) in the stomach, migration through the oesophagus, pharynx, trachea, related tissues (rhino-oropharynx), and cervical lymph nodes developing to the mature stage in any of these sites on days 9-20 post inoculation (P.I.). There was no parasite development up to the mature stage in cats inoculated orally with infective eggs, which indicates that the life cycle of this parasite includes an obligatory intermediate host. In one of the cats (fed carcass of infected mice) necropsied on day 43 P.I., it was observed the occurrence of the self-infective cycle of L. minor in the lung tissues and in the cervical region which was characterized by the finding of eggs in different stages of development, third stage larvae and mature worms. It's believed that some component of the carnivore gastrointestinal tracts may preclude the development of third stage larvae from L. minor eggs what explains the interruption of the life cycle in animals fed infective eggs. It's also pointed out the role of the intermediate host in the first stages of the life cycle of this helminth.
- Published
- 1992
- Full Text
- View/download PDF
41. [Nuclear syndrome of the oculomotor nerve caused by a mesencephalic infarction confirmed by MRI].
- Author
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Mateos V, Campos DM, Colosía VP, Salas-Puig J, Fernández JM, and Lahoz CH
- Subjects
- Cerebral Infarction pathology, Hemiplegia etiology, Humans, Male, Mesencephalon blood supply, Middle Aged, Syndrome, Thalamus blood supply, Tremor etiology, Cerebral Infarction complications, Magnetic Resonance Imaging, Mesencephalon pathology, Ophthalmoplegia etiology, Thalamus pathology
- Abstract
The nuclear syndrome of the third nerve was first described in 1981. It has the very characteristic disturbance of an ophthalmoplegia with complete ipsilateral third nerve palsy associated with paresis of elevation in contralateral eye. This particularly presentation is due to the innervation of the superior rectus which comes mainly from the contralateral oculomotor nucleus. As associated signs were described contralateral cerebellar and or pyramidal syndromes, uni or bilateral parasympathetic disfunction and sometimes gaze disorders. The etiology es usually a vascular damage (ischemic most frequently) located in mesencephalon. We report on a case of a 60 years old man who developed acute nuclear ophthalmoplegia of the third right nerve accompanied with cerebellar and pyramidal syndrome and focal asterixis in left extremities. MRI showed an ischemic lesion in right paramedial mesencephalic territory with extension to the ipsilateral thalamic region. Pyramidal and cerebellar syndromes and asterixis disappeared in a few weeks, while ophthalmoplegia remained unchanged. Semiologic characteristics and anatomic basis of the nuclear oculomotor syndrome which allow to make the differential diagnosis between this syndrome and intra-axial fascicular disturbances of the third nerve (Weber, Claude and Benedikt syndromes) are discuss.
- Published
- 1992
42. [Visceral larva migrans. Report of 3 cases].
- Author
-
Campos DM, Sousa MN, Zacariotti ET, Nardini R, Campos ML, Barbosa AP, Gebrin MC, and Lima FM
- Subjects
- Child, Enzyme-Linked Immunosorbent Assay, Female, Humans, Infant, Larva Migrans, Visceral complications, Male, Larva Migrans, Visceral diagnosis
- Abstract
We report three cases of children with visceral larva migrans confirmed by enzyme-linked immunosorbent assay (ELISA), in Goiânia. Pulmonary interstitial infiltrates with dry cough and peripheral eosinophilia characterised the first case. Clinical and laboratorial finding in the second case included dry cough, pulmonary infiltrates, peripheral eosinophilia and hypergammaglobulinaemia. A persistent eosinophilia, between 1987-1989, without apparent cause led to the confirmation of parasite infection in the third case.
- Published
- 1990
- Full Text
- View/download PDF
43. Phase II trial of aclarubicin in epidermoid carcinoma of the head and neck.
- Author
-
Carugati AA, Olivari AJ, Campos DM, Pradier R, and Woolley PV
- Subjects
- Aclarubicin, Adult, Aged, Antibiotics, Antineoplastic adverse effects, Clinical Trials as Topic, Drug Evaluation, Female, Humans, Male, Middle Aged, Naphthacenes adverse effects, Naphthacenes therapeutic use, Research Design, Antibiotics, Antineoplastic therapeutic use, Carcinoma, Squamous Cell drug therapy, Head and Neck Neoplasms drug therapy
- Published
- 1986
44. [In vitro effect of ivermectin on the eggs of Lagochilascaris minor Leiper, 1909].
- Author
-
Campos DM, Carneiro JR, and Souza LC
- Subjects
- Animals, Female, In Vitro Techniques, Ivermectin pharmacology, Nematoda growth & development, Ovum drug effects
- Published
- 1988
- Full Text
- View/download PDF
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