1. Antioxidant and Antiproliferative Activity of The Ethanolic Extract of Equisetum Myriochaetum and Molecular Docking of Its Main Metabolites (Apigenin, Kaempferol, and Quercetin) on β-Tubulin.
- Author
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Olazarán-Santibañez F, Rivera G, Vanoye-Eligio V, Mora-Olivo A, Aguirre-Guzmán G, Ramírez-Cabrera M, and Arredondo-Espinoza E
- Subjects
- Cell Line, Tumor, Ethanol chemistry, Female, Humans, Neoplasm Proteins metabolism, Tubulin metabolism, Antioxidants chemistry, Antioxidants pharmacology, Apigenin chemistry, Apigenin pharmacology, Cell Proliferation drug effects, Equisetum chemistry, Kaempferols chemistry, Kaempferols pharmacology, Molecular Docking Simulation, Neoplasm Proteins chemistry, Plant Extracts chemistry, Plant Extracts pharmacology, Quercetin chemistry, Quercetin pharmacology, Tubulin chemistry, Uterine Cervical Neoplasms chemistry, Uterine Cervical Neoplasms drug therapy, Uterine Cervical Neoplasms metabolism, Uterine Cervical Neoplasms pathology
- Abstract
Equisetum myriochaetum is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus Equisetum stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, E. myriochaetum ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of E. myriochaetum ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC
50 ) value of 6.78 μg/mL against SiHa cells.- Published
- 2021
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