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Pathogenicity of Vibrio penaeicida for white shrimp Litopenaeus vannamei: a cysteine protease-like exotoxin as a virulence factor.
- Source :
-
Diseases of aquatic organisms [Dis Aquat Organ] 2005 Nov 28; Vol. 67 (3), pp. 201-7. - Publication Year :
- 2005
-
Abstract
- The pathogenicity of Vibrio penaeicida Strains KH-1 and AM101, their culture-free supernatant (CFS), and their protein fraction obtained by 40% of ammonium sulfate precipitation (PFs40) were assessed in experimental challenges against juvenile Litopenaeus vannamei. Live Vibrio cells, CFS, and PFs40 from the AM101 strain produced a significantly higher mortality (p < 0.05) compared to the KH-1 strain. Toxicity and median lethal doses (LD50) of Fast Protein Liquid Chromatography (FPLC) products were evaluated on L. vannamei. The first FPLC fraction sample (A) from PFs40 of the AM101 strain displayed LD50 values of 1.68 and 5.61 microg protein ind.(-1), respectively. The second FPLC process from Fraction A showed a peak (A1) also with toxic effects to shrimp. PFs40, Fraction A, and Peak A1 showed a 38.5 kDa molecular band (SDS-PAGE), with activity on a gelatin protease zymogram. The lethal effect of PFs40 and Fraction A was inhibited by Proteinase K, CuCl2, E-64, and heat (60 and 100 degrees C) treatments, but was not inhibited by EDTA-Na2, aprotinin, and soy trypsin treatments. These results and the zymogram inhibition test suggest the presence of a cysteine protease-like proteinaceous exotoxin as a dominant protease, secreted by V. penaeicida Strain AM101.
- Subjects :
- Animals
Chromatography, Liquid
Copper
Cysteine Endopeptidases isolation & purification
Electrophoresis, Polyacrylamide Gel
Endopeptidase K
Hot Temperature
Lethal Dose 50
Leucine analogs & derivatives
Vibrio chemistry
Virulence Factors isolation & purification
Cysteine Endopeptidases toxicity
Penaeidae drug effects
Penaeidae microbiology
Vibrio pathogenicity
Virulence Factors toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0177-5103
- Volume :
- 67
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Diseases of aquatic organisms
- Publication Type :
- Academic Journal
- Accession number :
- 16408835
- Full Text :
- https://doi.org/10.3354/dao067201