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Results demonstrate that in donor bacteria containing conjugative plasmids, distinctive junctions are found between outer membranes of the mating cells. Data also indicate that the junctions are not composed of pilin.

The stages of cell plate development in cyrofixed tobacco BY-2 cell constitute the appearance of Golgi-derived vesicles between the phragmoplast microtubules, the generation of a tubulo-vesicular network, the combination of the network in a smooth tubular network. The network is then combined in a fenestrated, plate-like structure, characterized by the creation of finger-like projections that fuse with the parent cell plasma membrane, and followed by the closing of the plate fenestrae. Callose is the important lumenal constituent of forming cell plates, and on the membrane surface it creates a coat-like structure.

A waxy protective cuticle coats all primary aerial plant tissues. Its synthesis requires extensive export of lipids from epidermal cells to the plant surface. Arabidopsis cer5 mutants had reduced stem cuticular wax loads and accumulated sheetlike inclusions in the cytoplasm of wax-secreting cells. These inclusions represented abnormal deposits of cuticular wax and resembled inclusions found in a human disorder caused by a defective peroxisomal adenosine triphosphate binding cassette (ABC) transporter. We found that the CER5 gene encodes an ABC transporter localized in the plasma membrane of epidermal cells and conclude that it is required for wax export to the cuticle., All primary aerial organs of land plants are covered with a waxy cuticle that is essential for their protection and interaction with the environment. The cuticle is composed of very-long-chain [...]