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A gene encoding a novel flocculin related to the STA genes of yeast, which encode secreted glucoamylase was characterized. The STA genes comprise sequences that are homologous to the sporulation-specific glucoamylase SGA and to two other sequences, S2 and S1. Findings indicated that S2 and S1 are part of a single gene which has been named FLO11. FLO11 differs from all other yeast flocculins in that it is found near a centromere rather than a telomere and its expression is controlled by mating type.

Research has been conducted on histone H2B. The authors present the evidence for histone's ubiquitylation and deubiquitylation during gene activation, and identify H2B deubiquitylating enzyme in the Spt-Ada-Gcn5-Acetyltransferase complex.

Modification of histones is an important element in the regulation of gene expression. Previous work suggested a link between acetylation and phosphorylation, but questioned its mechanistic basis. We have purified a histone H3 serine-10 kinase complex from Saccharomyces cerevisiae and have identified its catalytic subunit as Snf1. The Snf1/AMPK family of kinases function in conserved signal transduction pathways. Our results show that Snf1 and the acetyltransferase Gcn5 function in an obligate sequence to enhance INO1 transcription by modifying histone H3 serine-10 and lysine-14. Thus, phosphorylation and acetylation are targeted to the same histone by promoterspecific regulation by a kinase/acetyltransferase pair, supporting models of gene regulation wherein transcription is controlled by coordinated patterns of histone modification., Posttranslational modifications of the [NH.sub.2]-terminal tails within core histones are important determinants of transcriptional regulation. In recent years, specific covalent modifications on histone tails have been characterized, including acetylation, phosphorylation, [...]