Search

Northern (RNA) blot analysis of the Bacillus subtilis biotin operon, bioWAFDBIorf2, indicated at least two steady-state polycistronic transcripts initiated from a putative vegetative (Pbio) promoter that precedes the operon. Biotin and the B. subtilis birA gene product regulated the synthesis of the transcripts. Also, replacing the putative Pbio promoter and regulatory sequence with a constitutive SP01 phage promoter produced a higher-level constitutive synthesis.

The cloning of metE gene encoding S-adenosylmethionine synthetase from Bacillus subtilis involves normal and inverse PCR. S-adenosylmethionine is a corepressor of methionine biosynthesis in B. subtilis. The metE gene present in the DNA sequence encodes a 400-amino acid sequence, which is homologous to S-adenosylmethionine synthetases. The cloned gene brings about the metE1 mutation and integrates at or near the chromosomal site of metE1.