Cloning and characterization of the metE gene encoding S-adenosylmethionine synthetase from Bacillus subtilis

The cloning of metE gene encoding S-adenosylmethionine synthetase from Bacillus subtilis involves normal and inverse PCR. S-adenosylmethionine is a corepressor of methionine biosynthesis in B. subtilis. The metE gene present in the DNA sequence encodes a 400-amino acid sequence, which is homologous to S-adenosylmethionine synthetases. The cloned gene brings about the metE1 mutation and integrates at or near the chromosomal site of metE1.