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1. Efficient targeted-selected mutagenesis in Caenorhabditis elegans: Toward a knockout for every gene

Author

Cuppen, Edwin, Gort, Eelke;, Hazendonk, Esther, Mudde, Josine, van de Belt, Jose, Nijman, Isaac J., Guryev, Victor, and Plasterk, Ronald H. A.

Subjects
Caenorhabditis elegans -- Genetic aspects, Gene mutations -- Research, Mutagenesis -- Research, Health
Abstract

A target-selected knockout approach, based on the random chemical mutagenesis is employed to study the efficient targeted-selected mutagenesis in Caenorhabditis elegans (C.elegans). The use of a nonsense introducing mutations (NIMs) genotyping approach for high-density microarrays is found to be extremely helpful for the genome-wide identification of C.elegans knockout.

Published
2007

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