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1. Large-scale cultivation of acidophilic hyperthermophiles for recovery of secreted proteins

Author

Worthington, Penny, Blum, Paul, Perez-Pomares, Francisco, and Elthon, Tom

Subjects
Bioreactors -- Design and construction, Bacteria, Thermophilic -- Growth, Amylases -- Research, Bacteriology -- Cultures and culture media, Company growth, Biological sciences
Abstract

This article describes simplified methodologies for the preparation and processing of acidophilic hyperthermophilic cultures. Research discusses the development of a low-cost bioreactor, a modified culture medium, and a high-throughput purification step utilizing a cation-exchange resin to cultivate and purify Sulfolobus solfataricus-secreted alpha-amylase.

Published
2003

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