Large-scale cultivation of acidophilic hyperthermophiles for recovery of secreted proteins

This article describes simplified methodologies for the preparation and processing of acidophilic hyperthermophilic cultures. Research discusses the development of a low-cost bioreactor, a modified culture medium, and a high-throughput purification step utilizing a cation-exchange resin to cultivate and purify Sulfolobus solfataricus-secreted alpha-amylase.