42 results
Search Results
2. Cancer-associated fibroblast-secreted collagen is associated with immune inhibitor receptor LAIR1 in gliomas
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Cancer -- Prevention ,Gliomas ,Collagen ,Brain tumors ,Health care industry - Abstract
To the Editor: A recently published JCI paper revealed that cancer-associated fibroblasts (CAFs) are present in glioblastoma and are defined by the presence of 9 transcriptional markers (1). CAFs can [...]
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- 2024
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3. Lipid droplets in the endothelium: the missing link between metabolic syndrome and cardiovascular disease?
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Jaffe, Iris Z. and Karumanchi, S. Ananth
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Endothelium -- Physiological aspects ,Triglycerides -- Physiological aspects ,Atherosclerosis -- Physiological aspects ,Hypertension -- Physiological aspects ,Health care industry ,Diseases ,Physiological aspects - Abstract
The physiology of lipid droplets (LDs) has been most extensively characterized in adipocytes, but LDs also accumulate in endothelial cells lining blood vessels in response to changing levels of triglycerides. In recent issues of the JCI, two independent papers highlight a direct role of endothelial LDs in the genesis of hypertension and atherosclerosis in rodent models. Kim et al. demonstrated that accumulation of LDs in the endothelium leads to hypertension, impairs endothelial function, and accelerates atherosclerosis. Boutagy, Gamez-Mendez, et al. knocked out Atgl in the endothelium and confirmed triglyceride accumulation in endothelial cells that was associated with reduced NO synthesis and impaired endothelial-dependent vasodilation. These data suggest that enhancing triglyceride breakdown in the endothelium could provide a treatment target for patients with metabolic syndrome., The pathophysiologic impact of excessive fatty acids Lipid droplets (LDs) are intracellular fat reservoirs that store neutral lipids for future use as an important energy source in metabolically active tissues [...]
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- 2024
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4. Clinical investigation of hypoxia-inducible factors: getting there
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Semenza, Gregg L.
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Hydroxylases -- Physiological aspects ,Health care industry ,Physiological aspects - Abstract
As part of the JCI's 100th-anniversary celebration, I reflect here on a few of the many papers published in JCI that have advanced our understanding of the physiological and pathological [...]
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- 2024
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5. Major breakthroughs in hematopoietic stem cell transplantation and future challenges in clinical implementation
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Kean, Leslie S. and Blazar, Bruce R.
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Hematopoietic stem cells -- Transplantation ,Market trend/market analysis ,Health care industry - Abstract
The Haitian proverb 'Beyond mountains, there are mountains' has many meanings. One that resonates for hematopoietic stem cell transplantation (HCT) over the last 50-plus years is the sense that as [...]
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- 2024
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6. Evolution of nasal and olfactory infection characteristics of SARS-CoV-2 variants
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Chen, Mengfei, Pekosz, Andrew, Villano, Jason S., Shen, Wenjuan, Zhou, Ruifeng, Kulaga, Heather, Li, Zhexuan, Smith, Amy, Gurung, Asiana, Beck, Sarah E., Witwer, Kenneth W., Mankowski, Joseph L., Ramanathan, Murugappan, Jr., Rowan, Nicholas R., and Lane, Andrew P.
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Company growth ,Health care industry - Abstract
SARS-CoV-2 infection of the upper airway and the subsequent immune response are early, critical factors in COVID-19 pathogenesis. By studying infection of human biopsies in vitro and in a hamster model in vivo, we demonstrated a transition in nasal tropism from olfactory to respiratory epithelium as the virus evolved. Analyzing each variant revealed that SARS-CoV-2 WA1 or Delta infect a proportion of olfactory neurons in addition to the primary target sustentacular cells. The Delta variant possessed broader cellular invasion capacity into the submucosa, while Omicron displayed enhanced nasal respiratory infection and longer retention in the sinonasal epithelium. The olfactory neuronal infection by WA1 and the subsequent olfactory bulb transport via axon were more pronounced in younger hosts. In addition, the observed viral clearance delay and phagocytic dysfunction in aged olfactory mucosa were accompanied by a decline of phagocytosis-related genes. Further, robust basal stem cell activation contributed to neuroepithelial regeneration and restored ACE2 expression postinfection. Together, our study characterized the nasal tropism of SARS- CoV-2 strains, immune clearance, and regeneration after infection. The shifting characteristics of viral infection at the airway portal provide insight into the variability of COVID-19 clinical features, particularly long COVID, and may suggest differing strategies for early local intervention., Introduction Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative pathogen in the worldwide pandemic of coronavirus disease 2019 (COVID-19), is readily transmitted via respiratory droplets during close contact. The [...]
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- 2024
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7. mTORCI controls murine postprandial hepatic glycogen synthesis via Ppp1r3b
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Uehara, Kahealani, Lee, Won Dong, Stefkovich, Megan, Biswas, Dipsikha, Santoleri, Dominic, Whitlock, Anna Garcia, Quinn, William, III, Coopersmith, Talia, Creasy, Kate Townsend, Rader, Daniel J., Sakamoto, Kei, Rabinowitz, Joshua D., and Titchenell, Paul M.
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Genetic polymorphisms -- Analysis ,Phosphorylation -- Health aspects ,Glycogen -- Synthesis ,Glucose metabolism -- Health aspects ,Health care industry - Abstract
In response to a meal, insulin drives hepatic glycogen synthesis to help regulate systemic glucose homeostasis. The mechanistic target of rapamycin complex 1 (mTORC1) is a well-established insulin target and contributes to the postprandial control of liver lipid metabolism, autophagy, and protein synthesis. However, its role in hepatic glucose metabolism is less understood. Here, we used metabolomics, isotope tracing, and mouse genetics to define a role for liver mTORC1 signaling in the control of postprandial glycolytic intermediates and glycogen deposition. We show that mTORC1 is required for glycogen synthase activity and glycogenesis. Mechanistically, hepatic mTORC1 activity promotes the feeding-dependent induction of Ppp1r3b, a gene encoding a phosphatase important for glycogen synthase activity whose polymorphisms are linked to human diabetes. Reexpression of Ppp1r3b in livers lacking mTORC1 signaling enhances glycogen synthase activity and restores postprandial glycogen content. mTORC1-dependent transcriptional control of Ppp1r3b is facilitated by FOXO1, a well characterized transcriptional regulator involved in the hepatic response to nutrient intake. Collectively, we identify a role for mTORC1 signaling in the transcriptional regulation of Ppp1r3b and the subsequent induction of postprandial hepatic glycogen synthesis., Introduction The liver is a central regulator of systemic glucose metabolism. Dysregulation of postprandial hepatic glucose metabolism contributes to the development of metabolic disorders, such as insulin resistance and type [...]
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- 2024
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8. Cytoplasmic retention of the DNA/RNA-binding protein FUS ameliorates organ fibrosis in mice
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Chiusa, Manuel, Lee, Youngmin A., Zhang, Ming-Zhi, Harris, Raymond C., Sherrill, Taylor, Lindner, Volkhard, Brooks, Craig R., Yu, Gang, Fogo, Agnes B., Flynn, Charles R., Zienkiewicz, Jozef, Hawiger, Jacek, Zent, Roy, and Pozzi, Ambra
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Cytoplasm -- Physiological aspects ,Fibrosis -- Development and progression -- Genetic aspects ,Translocation (Genetics) -- Health aspects ,DNA binding proteins -- Physiological aspects -- Health aspects ,Health care industry - Abstract
Uncontrolled accumulation of extracellular matrix leads to tissue fibrosis and loss of organ function. We previously demonstrated in vitro that the DNA/RNA-binding protein fused in sarcoma (FUS) promotes fibrotic responses by translocating to the nucleus, where it initiates collagen gene transcription. However, it is still not known whether FUS is profibrotic in vivo and whether preventing its nuclear translocation might inhibit development of fibrosis following injury. We now demonstrate that levels of nuclear FUS are significantly increased in mouse models of kidney and liver fibrosis. To evaluate the direct role of FUS nuclear translocation in fibrosis, we used mice that carry a mutation in the FUS nuclear localization sequence (FUSR521G) and the cell-penetrating peptide CP-FUS-NLS that we previously showed inhibits FUS nuclear translocation in vitro. We provide evidence that FUSR521G mice or CP-FUS-NLS-treated mice showed reduced nuclear FUS and fibrosis following injury. Finally, differential gene expression analysis and immunohistochemistry of tissues from individuals with focal segmental glomerulosclerosis or nonalcoholic steatohepatitis revealed significant upregulation of FUS and/or collagen genes and FUS protein nuclear localization in diseased organs. These results demonstrate that injury-induced nuclear translocation of FUS contributes to fibrosis and highlight CP-FUS-NLS as a promising therapeutic option for organ fibrosis., Introduction Organ fibrosis is characterized by excessive deposition of extracellular matrix (ECM) components (mainly collagen) within an injured organ that leads to the disruption of normal tissue architecture and loss [...]
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- 2024
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9. Bisphosphonates for osteoporosis: from bench to clinic
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Bellido, Teresita
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Diphosphonates -- Chemical properties -- Physiological aspects -- Usage ,Bone diseases -- Drug therapy ,Health care industry - Abstract
In honor of the 100th anniversary of the Journal of Clinical Investigation (JCI), I am pleased to provide this Viewpoint on bisphosphonates, which are, without doubt, one of the most [...]
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- 2024
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10. Contralateral versus ipsilateral vaccine boosting for COVID-19: considering the broader scientific landscape
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Goepfert, Paul
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Health care industry - Abstract
In the relentless battle against the COVID-19 pandemic, the deployment of mRNA vaccines has stood out as a beacon of hope. The successes of Pfizer-BioNTech NT162b2 and Moderna mRNA-1273 vaccines have been remarkable, marking a revolutionary advancement in the field of vaccinology. Despite their rapid development and impressive efficacy, challenges have emerged, particularly concerning the waning immune response over time and the evolving landscape of SARS-CoV-2 variants. The study published in this issue of JCI by Fazli et al. introduces an approach to potentially enhancing the immune responses generated by COVID-19 mRNA vaccines. The study meticulously examines the outcomes of nearly 1,000 participants who received one or two booster doses with the Pfizer-BioNTech NT162b2 vaccine either ipsilaterally or contralaterally in relation to the initial vaccine dose. Intriguingly, those who received the booster contralaterally exhibited a heightened antibody response that was particularly noteworthy in the later time points after boost., mRNA COVID-19 vaccination successes and limitations The advent of mRNA technology in vaccine development has been transformative, allowing scientists to respond rapidly to the urgent global need for effective COVID-19 [...]
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- 2024
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11. Mast cell activation disrupts interactions between endothelial cells and pericytes during early life allergic asthma
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Joulia, Regis, Puttur, Franz, Stolting, Helen, Traves, William J., Entwistle, Lewis J., Voitovich, Anastasia, Martin, Minerva Garcia, Al-Sahaf, May, Bonner, Katie, Scotney, Elizabeth, Molyneaux, Philip L., Hewitt, Richard J., Walker, Simone A., Yates, Laura, Saglani, Sejal, and Lloyd, Clare M.
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Asthma in children -- Development and progression -- Causes of ,Bronchi -- Physiological aspects -- Health aspects ,Allergic reaction -- Development and progression -- Complications and side effects ,Allergy -- Development and progression -- Complications and side effects ,Pediatric research ,Health care industry - Abstract
Allergic asthma generally starts during early life and is linked to substantial tissue remodeling and lung dysfunction. Although angiogenesis is a feature of the disrupted airway, the impact of allergic asthma on the pulmonary microcirculation during early life is unknown. Here, using quantitative imaging in precision-cut lung slices (PCLSs), we report that exposure of neonatal mice to house dust mite (HDM) extract disrupts endothelial cell/pericyte interactions in adventitial areas. Central to the blood vessel structure, the loss of pericyte coverage was driven by mast cell (MC) proteases, such as tryptase, that can induce pericyte retraction and loss of the critical adhesion molecule N-cadherin. Furthermore, spatial transcriptomics of pediatric asthmatic endobronchial biopsies suggests intense vascular stress and remodeling linked with increased expression of MC activation pathways in regions enriched in blood vessels. These data provide previously unappreciated insights into the pathophysiology of allergic asthma with potential long-term vascular defects., Introduction Allergic asthma commonly develops during early childhood. While it is difficult to precisely define the prevalence of children affected, it is now estimated that between 5% and 20% of [...]
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- 2024
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12. Contralateral second dose improves antibody responses to a 2-dose mRNA vaccination regimen
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Fazli, Sedigheh, Thomas, Archana, Estrada, Abram E., Ross, Hiro A.P., Lee, David Xthona, Kazmierczak, Steven, Slifka, Mark K., Montefiori, David, Messer, William B., and Curlin, Marcel E.
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Immune response -- Physiological aspects -- Health aspects ,Health care industry ,Comirnaty (Vaccine) -- Physiological aspects -- Dosage and administration - Abstract
BACKGROUND. Vaccination is typically administered without regard to site of prior vaccination, but this factor may substantially affect downstream immune responses. METHODS. We assessed serological responses to initial COVID-19 vaccination in baseline seronegative adults who received second-dose boosters in the ipsilateral or contralateral arm relative to initial vaccination. We measured serum SARSCoV-2 spike-specific Ig, receptor-binding domain-specific (RBD-specific) IgG, SARS-CoV-2 nucleocapsid-specific IgG, and neutralizing antibody titers against SARS-CoV-2.D614G (early strain) and SARS-CoV-2.B.1.1.529 (Omicron) at approximately 0.6, 8, and 14 months after boosting. RESULTS. In 947 individuals, contralateral boosting was associated with higher spike-specific serum Ig, and this effect increased over time, from a 1.1-fold to a 1.4-fold increase by 14 months (P < 0.001). A similar pattern was seen for RBD-specific IgG. Among 54 pairs matched for age, sex, and relevant time intervals, arm groups had similar antibody levels at study visit 2 (W2), but contralateral boosting resulted in significantly higher binding and neutralizing antibody titers at W3 and W4, with progressive increase over time, ranging from 1.3-fold (total Ig, P = 0.007) to 4.0-fold (pseudovirus neutralization to B.1.1.529, P < 0.001). CONCLUSIONS. In previously unexposed adults receiving an initial vaccine series with the BNT162b2 mRNA COVID-19 vaccine, contralateral boosting substantially increases antibody magnitude and breadth at times beyond 3 weeks after vaccination. This effect should be considered during arm selection in the context of multidose vaccine regimens. FUNDING. M.J. Murdock Charitable Trust, OHSU Foundation, NIH., Introduction In the 227 years since Edward Jenner demonstrated that inoculation with material from cowpox lesions could provide protection from symptomatic illness due to smallpox, vaccine research has advanced to [...]
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- 2024
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13. ZMYND8 protects breast cancer stem cells against oxidative stress and ferroptosis through activation of NRF2
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Luo, Maowu, Bao, Lei, Xue, Yuanyuan, Zhu, Ming, Kumar, Ashwani, Xing, Chao, Wang, Jennifer E., Wang, Yingfei, and Luo, Weibo
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Zinc finger proteins -- Physiological aspects -- Health aspects ,Stem cells -- Genetic aspects -- Physiological aspects -- Health aspects ,Breast cancer -- Development and progression -- Genetic aspects ,Transcription factors -- Physiological aspects -- Health aspects ,Health care industry - Abstract
Breast cancer stem cells (BCSCs) mitigate oxidative stress to maintain their viability and plasticity. However, the regulatory mechanism of oxidative stress in BCSCs remains unclear. We recently found that the histone reader ZMYND8 was upregulated in BCSCs. Here, we showed that ZMYND8 reduced ROS and iron to inhibit ferroptosis in aldehyde dehydrogenase-high ([ALDH.sup.hi]) BCSCs, leading to BCSC expansion and tumor initiation in mice. The underlying mechanism involved a two-fold posttranslational regulation of nuclear factor erythroid 2-related factor 2 (NRF2). ZMYND8 increased stability of NRF2 protein through KEAP1 silencing. On the other hand, ZMYND8 interacted with and recruited NRF2 to the promoters of antioxidant genes to enhance gene transcription in mammospheres. NRF2 phenocopied ZMYND8 to enhance BCSC stemness and tumor initiation by inhibiting ROS and ferroptosis. Loss of NRF2 counteracted ZMYND8's effects on antioxidant genes and ROS in mammospheres. Interestingly, ZMYND8 expression was directly controlled by NRF2 in mammospheres. Collectively, these findings uncover a positive feedback loop that amplifies the antioxidant defense mechanism sustaining BCSC survival and stemness., Introduction Breast cancer stem cells (BCSCs) are defined as a subpopulation of tumor cells with capability of self renewal and long-term maintenance of breast tumors and are responsible for breast [...]
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- 2024
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14. G protein-coupled receptors: from radioligand binding to cellular signaling
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Rockman, Howard A. and Lefkowitz, Robert J.
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Ligand binding (Biochemistry) -- Research ,Medical research ,Medicine, Experimental ,G proteins -- Health aspects -- Physiological aspects ,Cellular signal transduction -- Research ,Cell receptors -- Health aspects -- Physiological aspects ,Health care industry - Abstract
G protein-coupled receptors (GPCRs) represent by far the largest, most versatile, and ubiquitous class of cellular receptors, comprising more than 800 distinct receptors. They represent the largest class of targets [...]
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- 2024
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15. SGLT2 inhibitors: cardiorenal metabolic drugs for the ages
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DeFronzo, Ralph A.
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Hypoglycemic agents -- Research ,Metabolic diseases -- Drug therapy ,Cardiovascular diseases -- Drug therapy ,Pharmaceutical research ,Kidney diseases -- Drug therapy ,Health care industry - Abstract
When does the story start? Having trained as an endocrinologist and diabetologist, as well as a nephrologist interested in phosphate and glucose transport in the kidney (1), it was logical [...]
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- 2024
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16. Cancer neoepitopes viewed through negative selection and peripheral tolerance: a new path to cancer vaccines
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Srivastava, Pramod K.
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Immunological research ,Antigenic determinants -- Research ,Immunity -- Research ,Cancer vaccines -- Research ,Health care industry - Abstract
A proportion of somatic mutations in tumors create neoepitopes that can prime T cell responses that target the MHC I- neoepitope complexes on tumor cells, mediating tumor control or rejection. Despite the compelling centrality of neoepitopes to cancer immunity, we know remarkably little about what constitutes a neoepitope that can mediate tumor control in vivo and what distinguishes such a neoepitope from the vast majority of similar candidate neoepitopes that are inefficacious in vivo. Studies in mice as well as clinical trials have begun to reveal the unexpected paradoxes in this area. Because cancer neoepitopes straddle that ambiguous ground between self and non-self, some rules that are fundamental to immunology of frankly non-self antigens, such as viral or model antigens, do not appear to apply to neoepitopes. Because neoepitopes are so similar to self-epitopes, with only small changes that render them non- self, immune response to them is regulated at least partially the way immune response to self is regulated. Therefore, neoepitopes are viewed and understood here through the clarifying lens of negative thymic selection. Here, the emergent questions in the biology and clinical applications of neoepitopes are discussed critically and a mechanistic and testable framework that explains the complexity and translational potential of these wonderful antigens is proposed., Introduction Revolutionary advancements in high-throughput DNA-sequencing approaches led to the identification of numerous tumor-specific mutations, some of which generate new epitopes (neoepitopes). Tremendous effort has gone into leveraging these neoepitopes [...]
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- 2024
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17. The etiologies of Kawasaki disease
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Burns, Jane C.
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Kawasaki disease -- Causes of ,Health care industry - Abstract
Kawasaki disease (KD) is a systemic vasculitis that affects young children and can result in coronary artery aneurysms. The etiology is currently unknown, but new clues from the epidemiology of KD in Japan, the country of highest incidence, are beginning to shed light on what may trigger this acute inflammatory condition. Additional clues from the global changes in KD incidence during the COVID-19 pandemic, coupled with a new birth cohort study from Japan, point to the potential role of person-to-person transmission of an infectious agent. However, the rising incidence of KD in Japan, with coherent waves across the entire country, points to an increasing intensity of exposure that cannot be explained by person- to- person spread. This Review discusses new and historical observations that guide us toward a better understanding of KD etiology and explores hypotheses and interpretations that can provide direction for future investigations. Once the etiology of KD is determined, accurate diagnostic tests will become available, and new, less expensive, and more effective targeted therapies will likely be possible. Clearly, solving the mystery of the etiologies of KD remains a priority for pediatric research., The cause of Kawasaki disease (KD), the most common cause of acquired heart disease in children, remains a mystery after 50 years of investigation. The historical record suggests that KD [...]
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- 2024
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18. Effects of AFQ056 on language learning in fragile X syndrome
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Berry-Kravis, Elizabeth, Abbeduto, Leonard, Hagerman, Randi, Coffey, Christopher S., Cudkowicz, Merit, Erickson, Craig A., McDuffie, Andrea, Hessl, David, Ethridge, Lauren, Tassone, Flora, Kaufmann, Walter E., Friedmann, Katherine, Bullard, Lauren, Hoffmann, Anne, Veenstra-VanderWeele, Jeremy, Staley, Kevin, Klements, David, Moshinsky, Michael, Harkey, Brittney, Long, Jeff, Fedler, Janel, Klingner, Elizabeth, Ecklund, Dixie, Costigan, Michele, Huff, Trevis, and Pearson, Brenda
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Fragile X syndrome -- Drug therapy ,Language acquisition -- Research ,Nervous system agents -- Testing ,Metabotropic glutamate receptors -- Health aspects ,Pediatric research ,Health care industry - Abstract
BACKGROUND. FXLEARN, the first-ever large multisite trial of effects of disease- targeted pharmacotherapy on learning, was designed to explore a paradigm for measuring effects of mechanism-targeted treatment in fragile X syndrome (FXS). In FXLEARN, the effects of metabotropic glutamate receptor type 5 (mGluR5) negative allosteric modulator (NAM) AFQ056 on language learning were evaluated in 3- to 6-year-old children with FXS, expected to have more learning plasticity than adults, for whom prior trials of mGluR5 NAMs have failed. METHODS. After a 4-month single-blind placebo lead-in, participants were randomized 1:1 to AFQ056 or placebo, with 2 months of dose optimization to the maximum tolerated dose, then 6 months of treatment during which a language- learning intervention was implemented for both groups. The primary outcome was a centrally scored videotaped communication measure, the Weighted Communication Scale (WCS). Secondary outcomes were objective performance-based and parent- reported cognitive and language measures. RESULTS. FXLEARN enrolled 110 participants, randomized 99, and had 91 who completed the placebo-controlled period. Although both groups made language progress and there were no safety issues, the change in WCS score during the placebo-controlled period was not significantly different between the AFQ056 and placebo-treated groups, nor were there any significant between-group differences in change in any secondary measures. CONCLUSION. Despite the large body of evidence supporting use of mGluR5 NAMs in animal models of FXS, this study suggests that this mechanism of action does not translate into benefit for the human FXS population and that better strategies are needed to determine which mechanisms will translate from preclinical models to humans in genetic neurodevelopmental disorders. TRIAL REGISTRATION. ClincalTrials.gov NCT02920892. FUNDING SOURCES. NeuroNEXT network NIH grants U01NS096767, U24NS1072 00, U24NS107209, U01NS077323, U24NS107183, U24NS107168, U24NS107128, U24NS107199, U24NS107198, U24NS107166, U10NS077368, U01NS077366, U24NS107205, U01NS077179, and U01NS077352; NIH grant P50HD103526; and Novartis IIT grant AFQ056X2201T for provision of AFQ056., Introduction Fragile X syndrome (FXS) is the most common known single-gene cause of intellectual disability (ID) and autism spectrum disorder (ASD), with an estimated prevalence of about 1:4,000 to 5,000 [...]
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- 2024
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19. Serum amyloid A expression in liver promotes synovial macrophage activation and chronic arthritis via NFAT5
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Li, Meiling, Kim, Yu-Mi, Koh, Jung Hee, Park, Jihyun, Kwon, H. Moo, Park, Jong- Hwan, Jin, Jingchun, Park, Youngjae, Kim, Donghyun, and Kim, Wan-Uk
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Glycoproteins -- Health aspects -- Physiological aspects ,Synovial membranes -- Health aspects -- Physiological aspects ,Arthritis -- Development and progression ,Liver -- Health aspects -- Physiological aspects ,Macrophages -- Health aspects -- Physiological aspects ,Transcription factors -- Health aspects -- Physiological aspects ,Health care industry - Abstract
Nuclear factor of activated T-cells 5 (NFAT5), an osmo-sensitive transcription factor, can be activated by isotonic stimuli, such as infection. It remains unclear, however, whether NFAT5 is required for damage-associated molecular pattern- triggered (DAMP-triggered) inflammation and immunity. Here, we found that several DAMPs increased NFAT5 expression in macrophages. In particular, serum amyloid A (SAA), primarily generated by the liver, substantially upregulated NFAT5 expression and activity through TLR2/4-JNK signalling pathway. Moreover, the SAA-TLR2/4-NFAT5 axis promoted migration and chemotaxis of macrophages in an IL-6- and chemokine ligand 2-dependent (CCL2-dependent) manner in vitro. Intraarticular injection of SAA markedly accelerated macrophage infiltration and arthritis progression in mice. By contrast, genetic ablation of NFAT5 or TLR2/4 rescued the pathology induced by SAA, confirming the SAA-TLR2/4-NFAT5 axis in vivo. Myeloid-specific depletion of NFAT5 also attenuated SAA-accelerated arthritis. Of note, inflammatory arthritis in mice strikingly induced SAA overexpression in the liver. Conversely, forced overexpression of the SAA gene in the liver accelerated joint damage, indicating that the liver contributes to bolstering chronic inflammation at remote sites by secreting SAA. Collectively, this study underscores the importance of the SAA-TLR2/4-NFAT5 axis in innate immunity, suggesting that acute phase reactant SAA mediates mutual interactions between liver and joints and ultimately aggravates chronic arthritis by enhancing macrophage activation., Introduction Macrophages play a pivotal role in the pathogenesis of chronic inflammatory diseases, such as rheumatoid arthritis (RA), with broad proinflammatory, destructive, and remodeling capabilities (1, 2). Similar to other [...]
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- 2024
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20. Autophagy differentially regulates tissue tolerance of distinct target organs in graft-versus-host disease models
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Oravecz-Wilson, Katherine, Lauder, Emma, Taylor, Austin, Maneix, Laure, Van Nostrand, Jeanine L., Sun, Yaping, Li, Lu, Zhao, Dongchang, Liu, Chen, and Reddy, Pavan
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Immunological research ,Tissues -- Health aspects -- Physiological aspects ,Immune response -- Research ,Autophagy (Cytology) -- Research ,Graft versus host reaction -- Models -- Development and progression ,Health care industry - Abstract
Tissue-intrinsic mechanisms that regulate severity of systemic pathogenic immune-mediated diseases, such as acute graft-versus-host disease (GVHD), remain poorly understood. Following allogeneic hematopoietic stem cell transplantation, autophagy, a cellular stress protective response, is induced in host nonhematopoietic cells. To systematically address the role of autophagy in various host nonhematopoietic tissues, both specific classical target organs of acute GVHD (intestines, liver, and skin) and organs conventionally not known to be targets of GVHD (kidneys and heart), we generated mice with organ-specific knockout of autophagy related 5 (ATG5) to specifically and exclusively inhibit autophagy in the specific organs. When compared with wild-type recipients, animals that lacked ATG5 in the gastrointestinal tract or liver showed significantly greater tissue injury and mortality, while autophagy deficiency in the skin, kidneys, or heart did not affect mortality. Treatment with the systemic autophagy inducer sirolimus only partially mitigated GVHD mortality in intestine-specific autophagy- deficient hosts. Deficiency of autophagy increased MHC class I on the target intestinal epithelial cells, resulting in greater susceptibility to damage by alloreactive T cells. Thus, autophagy is a critical cell-intrinsic protective response that promotes tissue tolerance and regulates GVHD severity., Introduction Graft-versus-host disease (GVHD) is a complex pathogenic immunological consequence of allogeneic hematopoietic stem cell transplantation (allo-HSCT) that causes injury to specific organs like the gastrointestinal (GI) tract, liver, and [...]
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- 2024
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21. Glucocorticoid receptor-dependent therapeutic efficacy of tauroursodeoxycholic acid in preclinical models of spinocerebellar ataxia type 3
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Duarte-Silva, Sara, Da Silva, Jorge Diogo, Monteiro-Fernandes, Daniela, Costa, Marta Daniela, Neves-Carvalho, Andreia, Raposo, Mafalda, Soares-Cunha, Carina, Correia, Joana S., Nogueira- Goncalves, Goncalo, Fernandes, Henrique S., Oliveira, Stephanie, Ferreira-Fernandes, Ana Rita, Rodrigues, Fernando, Pereira-Sousa, Joana, Vilasboas-Campos, Daniela, Guerreiro, Sara, Campos, Jonas, Meireles- Costa, Liliana, Rodrigues, Cecilia M.P., Cabantous, Stephanie, Sousa, Sergio F., Lima, Manuela, Teixeira-Castro, Andreia, and Maciel, Patricia
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Spinocerebellar ataxia -- Models -- Care and treatment -- Development and progression ,Bile acids -- Health aspects -- Testing ,Health care industry - Abstract
Spinocerebellar ataxia type 3 (SCA3) is an adult-onset neurodegenerative disease caused by a polyglutamine expansion in the ataxin-3 (ATXN3) gene. No effective treatment is available for this disorder, other than symptom-directed approaches. Bile acids have shown therapeutic efficacy in neurodegenerative disease models. Here, we pinpointed tauroursodeoxycholic acid (TUDCA) as an efficient therapeutic, improving the motor and neuropathological phenotype of SCA3 nematode and mouse models. Surprisingly, transcriptomic and functional in vivo data showed that TUDCA acts in neuronal tissue through the glucocorticoid receptor (GR), but independently of its canonical receptor, the farnesoid X receptor (FXR). TUDCA was predicted to bind to the GR, in a similar fashion to corticosteroid molecules. GR levels were decreased in disease-affected brain regions, likely due to increased protein degradation as a consequence of ATXN3 dysfunction being restored by TUDCA treatment. Analysis of a SCA3 clinical cohort showed intriguing correlations between the peripheral expression of GR and the predicted age at disease onset in presymptomatic subjects and FKBP5 expression with disease progression, suggesting this pathway as a potential source of biomarkers for future study. We have established a novel in vivo mechanism for the neuroprotective effects of TUDCA in SCA3 and propose this readily available drug for clinical trials in SCA3 patients., Introduction Neurodegenerative diseases are currently considered a growing epidemic in the aging population (1, 2), still lacking effective therapeutic options. For those disorders with an identified genetic cause, research has [...]
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- 2024
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22. KCTD1/KCTD15 complexes control ectodermal and neural crest cell functions, and their impairment causes aplasia cutis
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Raymundo, Jackelyn R., Zhang, Hui, Smaldone, Giovanni, Zhu, Wenjuan, Daly, Kathleen E., Glennon, Benjamin J., Pecoraro, Giovanni, Salvatore, Marco, Devine, William A., Lo, Cecilia W., Vitagliano, Luigi, and Marneros, Alexander G.
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Thermo Fisher Scientific Inc. ,Medical research ,Medicine, Experimental ,Skin -- Genetic aspects ,Health care industry ,Genetic aspects - Abstract
Aplasia cutis congenita (ACC) is a congenital epidermal defect of the midline scalp and has been proposed to be due to a primary keratinocyte abnormality. Why it forms mainly at this anatomic site has remained a long-standing enigma. KCTD1 mutations cause ACC, ectodermal abnormalities, and kidney fibrosis, whereas KCTD15 mutations cause ACC and cardiac outflow tract abnormalities. Here, we found that KCTD1 and KCTD15 can form multimeric complexes and can compensate for each other's loss and that disease mutations are dominant negative, resulting in lack of KCTD1/KCTD15 function. We demonstrated that KCTD15 is critical for cardiac outflow tract development, whereas KCTD1 regulates distal nephron function. Combined inactivation of KCTD1/KCTD15 in keratinocytes resulted in abnormal skin appendages but not in ACC. Instead, KCTD1/KCTD15 inactivation in neural crest cells resulted in ACC linked to midline skull defects, demonstrating that ACC is not caused by a primary defect in keratinocytes but is a secondary consequence of impaired cranial neural crest cells, giving rise to midline cranial suture cells that express keratinocyte-promoting growth factors. Our findings explain the clinical observations in patients with KCTD1 versus KCTD15 mutations, establish KCTD1/KCTD15 complexes as critical regulators of ectodermal and neural crest cell functions, and define ACC as a neurocristopathy., Introduction Aplasia cutis congenita (ACC) manifests with a congenital localized epidermal thinning (membranous ACC) or skin wound most commonly along the midline and vertex area of the scalp (1). Absence [...]
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- 2024
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23. The interaction of Synapsin 2a and Synaptogyrin-3 regulates fear extinction in mice
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Shen, Xi-Ya, Zhang, Juan, Huang, He-Zhou, Li, Shao-Dan, Zhou, Ling, Wu, Shi-Ping, Tang, Cheng, Huang, Xian, Liu, Zhi-Qiang, Guo, Zi-Yuan, Li, Xiang, Man, Heng-Ye, Lu, You-Ming, Zhu, Ling-Qiang, and Liu, Dan
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Neurons -- Health aspects ,Drug approval -- Health aspects ,Health care industry ,Health aspects - Abstract
The mechanisms behind a lack of efficient fear extinction in some individuals are unclear. Here, by employing a principal components analysis-based approach, we differentiated the mice into extinction- resistant and susceptible groups. We determined that elevated synapsin 2a (Syn2a) in the infralimbic cortex (IL) to basolateral amygdala (BLA) circuit disrupted presynaptic orchestration, leading to an excitatory/inhibitory imbalance in the BLA region and causing extinction resistance. Overexpression or silencing of Syn2a levels in IL neurons replicated or alleviated behavioral, electrophysiological, and biochemical phenotypes in resistant mice. We further identified that the proline-rich domain H in the C-terminus of Syn2a was indispensable for the interaction with synaptogyrin-3 (Syngr3) and demonstrated that disrupting this interaction restored extinction impairments. Molecular docking revealed that ritonavir, an FDA- approved HIV drug, could disrupt Syn2a- Syngr3 binding and rescue fear extinction behavior in Syn2a-elevated mice. In summary, the aberrant elevation of Syn2a expression and its interaction with Syngr3 at the presynaptic site were crucial in fear extinction resistance, suggesting a potential therapeutic avenue for related disorders., Introduction Posttraumatic stress disorder (PTSD) is a debilitating neuropsychiatric disorder (1) with complicated psychological and neurobiological mechanisms involving varied brain circuits mediating stress and fear responses (2-4), as well as [...]
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- 2024
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24. Dynamic metabolism of endothelial triglycerides protects against atherosclerosis in mice
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Boutagy, Nabil E., Gamez-Mendez, Ana, Fowler, Joseph W.M., Zhang, Hanming, Chaube, Bal K., Esplugues, Enric, Kuo, Andrew, Lee, Sungwoon, Horikami, Daiki, Zhang, Jiasheng, Citrin, Kathryn M., Singh, Abhishek K., Coon, Brian G., Lee, Monica Y., Suarez, Yajaira, Fernandez-Hernando, Carlos, and Sessa, William C.
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Lipids -- Physiological aspects ,Chronic diseases -- Physiological aspects ,Endothelium -- Physiological aspects ,Antilipemic agents -- Physiological aspects ,Lipase -- Physiological aspects ,Nitric oxide -- Physiological aspects ,Blood circulation disorders -- Physiological aspects ,Atherosclerosis -- Physiological aspects ,Health care industry ,Physiological aspects - Abstract
Blood vessels are continually exposed to circulating lipids, and elevation of ApoB-containing lipoproteins causes atherosclerosis. Lipoprotein metabolism is highly regulated by lipolysis, largely at the level of the capillary endothelium lining metabolically active tissues. How large blood vessels, the site of atherosclerotic vascular disease, regulate the flux of fatty acids (FAs) into triglyceride-rich (TG-rich) lipid droplets (LDs) is not known. In this study, we showed that deletion of the enzyme adipose TG lipase (ATGL) in the endothelium led to neutral lipid accumulation in vessels and impaired endothelialdependent vascular tone and nitric oxide synthesis to promote endothelial dysfunction. Mechanistically, the loss of ATGL led to endoplasmic reticulum stress-induced inflammation in the endothelium. Consistent with this mechanism, deletion of endothelial ATGL markedly increased lesion size in a model of atherosclerosis. Together, these data demonstrate that the dynamics of FA flux through LD affects endothelial cell homeostasis and consequently large vessel function during normal physiology and in a chronic disease state., Introduction The vascular endothelium is essential in lipid partitioning (1). Specifically, in metabolically active tissues (e.g., heart, skeletal muscle, adipose), triglyceride-rich (TG-rich) lipoproteins (chylomicrons, VLDL) are metabolized by lipoprotein lipase [...]
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- 2024
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25. Heterozygous mutations in the C-terminal domain of COPA underlie a complex autoinflammatory syndrome
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Delafontaine, Selket, Iannuzzo, Alberto, Bigley, Tarin M., Mylemans, Bram, Rana, Ruchit, Baatsen, Pieter, Poli, Maria Cecilia, Rymen, Daisy, Jansen, Katrien, Mekahli, Djalila, Casteels, Ingele, Cassiman, Catherine, Demaerel, Philippe, Lepelley, Alice, Fremond, Marie-Louise, Schrijvers, Rik, Vints, Xavier Bossuy Katlijn, Huybrechts, Wim, Tacine, Rachida, Willekens, Karen, Corveleyn, Anniek, Boeckx, Bram, Baggio, Marco, Ehlers, Lisa, Munck, Sebastian, Lambrechts, Diether, Moens, Arnout VoeLeen, Bucciol, Giorgia, Cooper, Megan A., Davis, Carla M., Delon, Jerome, and Meyts, Isabelle
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Interferon ,Family ,Autoimmunity ,B cells -- Genetic aspects ,Health care industry ,International economic relations ,Genetic aspects - Abstract
Mutations in the N-terminal WD40 domain of coatomer protein complex subunit a (COPA) cause a type I interferonopathy, typically characterized by alveolar hemorrhage, arthritis, and nephritis. We described 3 heterozygous mutations in the C-terminal domain (CTD) of COPA (p.C1013S, p.R1058C, and p.R1142X) in 6 children from 3 unrelated families with a similar syndrome of autoinflammation and autoimmunity. We showed that these CTD COPA mutations disrupt the integrity and the function of coat protein complex I (COPI). In [COPA.sup.R1142X] and [COPA.sup.R1058C] fibroblasts, we demonstrated that COPI dysfunction causes both an anterograde ER-to-Golgi and a retrograde Golgi-to-ER trafficking defect. The disturbed intracellular trafficking resulted in a cGAS/STING-dependent upregulation of the type I IFN signaling in patients and patient-derived cell lines, albeit through a distinct molecular mechanism in comparison with mutations in the WD40 domain of COPA. We showed that CTD COPA mutations induce an activation of ER stress and NF-[kappa]B signaling in patient-derived primary cell lines. These results demonstrate the importance of the integrity of the CTD of COPA for COPI function and homeostatic intracellular trafficking, essential to ER homeostasis. CTD COPA mutations result in disease by increased ER stress, disturbed intracellular transport, and increased proinflammatory signaling., Introduction The COPA gene encodes coatomer protein complex subunit [alpha] (COPA) (1). COPA was described in 1991 as a coat subunit of Golgi derived non-clathrin-coated vesicles, later termed 'coatomer' (2, [...]
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- 2024
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26. Alcohol-associated liver disease
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Mackowiak, Bryan, Fu, Yaojie, Maccioni, Luca, and Gao, Bin
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Oncology, Experimental -- Health aspects ,B cells -- Health aspects ,Liver cancer -- Development and progression -- Drug therapy ,Drug approval -- Health aspects ,Cancer -- Research ,Liver cirrhosis -- Drug therapy -- Development and progression ,Health care industry ,Drug therapy ,Development and progression ,Health aspects - Abstract
Alcohol-associated liver disease (ALD) is a major cause of chronic liver disease worldwide, and comprises a spectrum of several different disorders, including simple steatosis, steatohepatitis, cirrhosis, and superimposed hepatocellular carcinoma. Although tremendous progress has been made in the field of ALD over the last 20 years, the pathogenesis of ALD remains obscure, and there are currently no FDA-approved drugs for the treatment of ALD. In this Review, we discuss new insights into the pathogenesis and therapeutic targets of ALD, utilizing the study of multiomics and other cutting-edge approaches. The potential translation of these studies into clinical practice and therapy is deliberated. We also discuss preclinical models of ALD, interplay of ALD and metabolic dysfunction, alcohol-associated liver cancer, the heterogeneity of ALD, and some potential translational research prospects for ALD., Introduction Alcohol-associated liver disease (ALD), which replaces the former name 'alcoholic liver disease' to avoid the use of the stigmatizing word 'alcoholic,' is one of most common chronic liver diseases [...]
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- 2024
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27. Multiomic profiling reveals metabolic alterations mediating aberrant platelet activity and inflammation in myeloproliferative neoplasms
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He, Fan, Laranjeira, Angelo B.A., Kong, Tim, Lin, Shuyang, Ashworth, Katrina J., Liu, Alice, Lasky, Nina M., Fisher, Daniel A.C., Cox, Maggie J., Fulbright, Mary C., Antunes-Heck, Lilian, Yu, LaYow, Brakhane, Molly, Gao, Bei, Sykes, Stephen M., D'Alessandro, Angelo, Di Paola, Jorge, and Oh, Stephen T.
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Washington University. School of Medicine ,Tumors ,Inflammation ,Health care industry - Abstract
Platelets from patients with myeloproliferative neoplasms (MPNs) exhibit a hyperreactive phenotype. Here, we found elevated P-selectin exposure and platelet-leukocyte aggregates indicating activation of platelets from essential thrombocythemia (ET) patients. Single-cell RNA-seq analysis of primary samples revealed significant enrichment of transcripts related to platelet activation, mTOR, and oxidative phosphorylation in ET patient platelets. These observations were validated via proteomic profiling. Platelet metabolomics revealed distinct metabolic phenotypes consisting of elevated ATP generation accompanied by increases in the levels of multiple intermediates of the tricarboxylic acid cycle, but lower a-ketoglutarate ([alpha]-KG) in MPN patients. Inhibition of PI3K/AKT/mTOR signaling significantly reduced metabolic responses and hyperreactivity in MPN patient platelets, while [alpha]-KG supplementation markedly reduced oxygen consumption and ATP generation. Ex vivo incubation of platelets from both MPN patients and Jak2 V617F-knockin mice with [alpha]-KG supplementation significantly reduced platelet activation responses. Oral [alpha]-KG supplementation of Jak2 V617Fmice decreased splenomegaly and reduced hematocrit, monocyte, and platelet counts. Finally, [alpha]- KG treatment significantly decreased proinflammatory cytokine secretion from MPN [CD14.sup.+] monocytes. Our results reveal a previously unrecognized metabolic disorder in conjunction with aberrant PI3K/AKT/mTOR signaling that contributes to platelet hyperreactivity in MPN patients., Introduction Philadelphia-negative chronic myeloproliferative neoplasms (MPNs), including polycythemia vera (PV), essential thrombocythemia (ET), and myelofibrosis (MF), are clonal hematopoietic disorders characterized by overproduction of mature blood cells such as erythrocytes, [...]
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- 2024
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28. Disease-associated AIOLOS variants lead to immune deficiency/dysregulation by haploinsufficiency and redefine AIOLOS functional domains
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Kuehn, Hye Sun, Sakovich, Inga S., Niemela, Julie E., Silva, Agustin A. Gil, Stoddard, Jennifer L., Polyakova, Ekaterina A., Sole, Ana Esteve, Aleshkevich, Svetlana N., Uglova, Tatjana A., Belevtsev, Mikhail V., Vertelko, Vladislav R., Shman, Tatsiana V., Kupchinskaya, Aleksandra N., Walter, Jolan E., Fleisher, Thomas A., Notarangelo, Luigi D., Peng, Xiao P., Delmonte, Ottavia M., Sharapova, Svetlana O., and Rosenzweig, Sergio D.
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Thermo Fisher Scientific Inc. ,Becton, Dickinson and Co. ,United States. National Institutes of Health ,Scientific equipment and supplies industry ,DNA-ligand interactions ,Omalizumab ,Medical test kit industry ,Autoimmunity ,Allergic reaction ,B cells ,Medical equipment and supplies industry ,Allergy ,DNA binding proteins ,Health care industry ,Diseases - Abstract
AIOLOS, also known as IKZF3, is a transcription factor that is highly expressed in the lymphoid lineage and is critical for lymphocyte differentiation and development. Here, we report on 9 individuals from 3 unrelated families carrying AIOLOS variants Q402* or E82K, which led to AIOLOS haploinsufficiency through different mechanisms of action. Nonsense mutant Q402* displayed abnormal DNA binding, pericentromeric targeting, posttranscriptional modification, and transcriptome regulation. Structurally, the mutant lacked the AIOLOS zinc finger (ZF) 5-6 dimerization domain, but was still able to homodimerize with WT AIOLOS and negatively regulate DNA binding through ZF1, a previously unrecognized function for this domain. Missense mutant E82K showed overall normal AIOLOS functions; however, by affecting a redefined AIOLOS protein stability domain, it also led to haploinsufficiency. Patients with AIOLOS haploinsufficiency showed hypogammaglobulinemia, recurrent infections, autoimmunity, and allergy, but with incomplete clinical penetrance. Altogether, these data redefine the AIOLOS structure-function relationship and expand the spectrum of AIOLOS- associated diseases., Introduction The IKAROS family members consist of 5 zinc-finger (ZF) transcription factors encoded by IKZF1 to IKZF5. IKAROS (IKZF1), HELIOS (IKZF2), and AIOLOS (IKZF3) are highly expressed in the lymphoid [...]
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- 2024
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29. Single-cell sequencing reveals Hippo signaling as a driver of fibrosis in hidradenitis suppurativa
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van Straalen, Kelsey R., Ma, Feiyang, Tsou, Pei-Suen, Plazyo, Olesya, Gharaee-Kermani, Mehrnaz, Calbet, Marta, Xing, Xianying, Sarkar, Mrinal K., Uppala, Ranjitha, Harms, Paul W., Wasikowski, Rachael, Nahlawi, Lina, Nakamura, Mio, Eshaq, Milad, Wang, Cong, Dobry, Craig, Kozlow, Jeffrey H., Cherry-Bukowiec, Jill, Brodie, William D., Wolk, Kerstin, Uluckan, Ozge, Mattichak, Megan N., Pellegrini, Matteo, Modlin, Robert L., Maverakis, Emanual, Sabat, Robert, Kahlenberg, J. Michelle, Billi, Allison C., Tsoi, Lam C., and Gudjonsson, Johann E.
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University of Michigan. Medical School ,Verteporfin ,Fibrosis ,RNA sequencing ,Immune response ,B cells ,Adalimumab ,Health care industry - Abstract
Hidradenitis suppurativa (HS) is a chronic inflammatory disease characterized by abscesses, nodules, dissecting/ draining tunnels, and extensive fibrosis. Here, we integrate single-cell RNA sequencing, spatial transcriptomics, and immunostaining to provide an unprecedented view of the pathogenesis of chronic HS, characterizing the main cellular players and defining their interactions. We found a striking layering of the chronic HS infiltrate and identified the contribution of 2 fibroblast subtypes ([SFRP4.sup.+] and [CXCL13.sup.+]) in orchestrating this compartmentalized immune response. We further demonstrated the central role of the Hippo pathway in promoting extensive fibrosis in HS and provided preclinical evidence that the profibrotic fibroblast response in HS can be modulated through inhibition of this pathway. These data provide insights into key aspects of HS pathogenesis with broad therapeutic implications., Introduction Hidradenitis suppurativa (HS) is a chronic inflammatory disease of the skin that affects 1% of the general population (1). The disease is characterized by acute, recurrent inflammatory nodules and [...]
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- 2024
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30. A conversation with Olufunmilayo Olopade
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Neill, Ushma S.
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Cancer -- Genetic aspects ,Physicians -- Interviews - Abstract
Physician and geneticist Dr. Funmi Olopade is the founding director of the Center for Cancer Genetics and Global Health at the University of Chicago (Figure 1). Olopade's research is focused [...]
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- 2024
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31. A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III
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Gardin, Antoine, Rouillon, Jeremy, Montalvo-Romeral, Valle, Rossiaud, Lucille, Vidal, Patrice, Launay, Romain, Vie, Mallaury, Benchekroun, Youssef Krimi, Cosette, Jeremie, Bertin, Berangere, La Bella, Tiziana, Dubreuil, Guillaume, Nozi, Justine, Jauze, Louisa, Fragnoud, Romain, Daniele, Nathalie, Van Wittenberghe, Laetitia, Esque, Jeremy, Andre, Isabelle, Nissan, Xavier, Hoch, Lucile, and Ronzitti, Giuseppe
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Glycogen metabolism -- Genetic aspects ,Gene therapy -- Methods ,Glycogenosis -- Drug therapy ,Dependoviruses -- Usage ,Health care industry ,Drug therapy ,Usage ,Genetic aspects ,Methods - Abstract
Glycogen storage disease type III (GSDIII) is a rare inborn error of metabolism affecting liver, skeletal muscle, and heart due to mutations of the AGL gene encoding for the glycogen debranching enzyme (GDE). No curative treatment exists for GSDIII. The 4.6 kb GDE cDNA represents the major technical challenge toward the development of a single recombinant adeno- associated virus-derived (rAAV-derived) vector gene therapy strategy. Using information on GDE structure and molecular modeling, we generated multiple truncated GDEs. Among them, an N-terminal-truncated mutant, [DELTA]Nter2-GDE, had a similar efficacy in vivo compared with the full-size enzyme. A rAAV vector expressing [DELTA]Nter2- GDE allowed significant glycogen reduction in heart and muscle of [Agl.sup.-/-] mice 3 months after i.v. injection, as well as normalization of histology features and restoration of muscle strength. Similarly, glycogen accumulation and histological features were corrected in a recently generated [Agl.sup.-/-] rat model. Finally, transduction with rAAV vectors encoding [DELTA]Nter2-GDE corrected glycogen accumulation in an in vitro human skeletal muscle cellular model of GSDIII. In conclusion, our results demonstrated the ability of a single rAAV vector expressing a functional mini-GDE transgene to correct the muscle and heart phenotype in multiple models of GSDIII, supporting its clinical translation to patients with GSDIII., Introduction Glycogen storage disease type III (GSDIII) is a rare inborn error of metabolism, with an incidence of 1 in 100,000, caused by mutations in the AGL gene, which encodes [...]
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- 2024
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32. Interactions among insulin resistance, epigenetics, and donor sex in gene expression regulation of iPSC- derived myoblasts
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Haider, Nida and Kahn, C. Ronald
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Stem cells -- Genetic aspects -- Physiological aspects ,Insulin resistance -- Genetic aspects -- Risk factors ,Sex factors in disease -- Genetic aspects ,Epigenetic inheritance -- Physiological aspects -- Health aspects - Abstract
About 25% of people in the general population are insulin resistant, increasing the risk for type 2 diabetes (T2D) and metabolic disease. Transcriptomic analysis of induced pluripotent stem cells differentiated into myoblasts (iMyos) from insulin-resistant (I-Res) versus insulin-sensitive (I-Sen) nondiabetic individuals revealed that 306 genes increased and 271 genes decreased in expression in iMyos from I-Res donors with differences of 2- fold or more. Over 30 of the genes changed in I-Res iMyos were associated with T2D by SNPs and were functionally linked to insulin action and control of metabolism. Interestingly, we also identified more than 1,500 differences in gene expression that were dependent on the sex of the cell donor, some of which modified the insulin resistance effects. Many of these sex differences were associated with increased DNA methylation in cells from female donors and were reversed by 5-azacytidine. By contrast, the insulin sensitivity differences were not reversed and thus appear to reflect genetic or methylation- independent epigenetic effects., Introduction Insulin resistance underlies the development of type 2 diabetes (T2D) and metabolic syndrome (1-3), which together affect 20%-30% of adults in Westernized populations (4). In addition, an almost equal [...]
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- 2024
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33. Chronic graft-versus-host disease detected by tissue-specific cell-free DNA methylation biomarkers
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Avni, Batia, Neiman, Daniel, Shaked, Elior, Gal-Rosenberg, Ofer, Grisariu, Sigal, Kuzli, Mona, Avni, Ilai, Fracchia, Andrea, Stepensky, Polina, Zuckerman, Tsila, Lev-Sagie, Ahinoam, Fox-Fisher, Ilana, Piyanzin, Sheina, Moss, Joshua, Salpeter, Seth J., Glaser, Benjamin, Shemer, Ruth, and Dor, Yuval
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Immunological research ,Methylation -- Research ,Graft versus host reaction -- Diagnosis ,Hematopoietic stem cells -- Transplantation ,Extrachromosomal DNA -- Measurement -- Health aspects ,Biological markers -- Research - Abstract
BACKGROUND. Accurate detection of graft-versus-host disease (GVHD) is a major challenge in the management of patients undergoing hematopoietic stem cell transplantation (HCT). Here, we demonstrated the use of circulating cell-free DNA (cfDNA) for detection of tissue turnover and chronic GVHD (cGVHD) in specific organs. METHODS. We established a cocktail of tissue-specific DNA methylation markers and used it to determine the concentration of cfDNA molecules derived from the liver, skin, lungs, colon, and specific immune cells in 101 patients undergoing HCT. RESULTS. Patients with active cGVHD showed elevated concentrations of cfDNA, as well as tissue-specific methylation markers that agreed with clinical scores. Strikingly, transplanted patients with no clinical symptoms had abnormally high levels of tissue-specific markers, suggesting hidden tissue turnover even in the absence of evident clinical pathology. An integrative model taking into account total cfDNA concentration, monocyte/macrophage cfDNA levels and alanine transaminase was able to correctly identify GVHD with a specificity of 86% and precision of 89% (AUC of 0.8). CONCLUSION. cfDNA markers can be used for the detection of cGVHD, opening a window into underlying tissue dynamics in patients that receive allogeneic stem cell transplants. FUNDING. This work was supported by grants from the Ernest and Bonnie Beutler Research Program of Excellence in Genomic Medicine, The Israel Science Foundation, the Waldholtz/Pakula family, the Robert M. and Marilyn Sternberg Family Charitable Foundation and the Helmsley Charitable Trust (to YD)., Introduction Hematopoietic stem cell transplantation (HCT) is an essential and often the sole curative treatment strategy for high risk hematologic malignancies (1). Graft-versus-host disease (GVHD), the foremost complication of allogeneic [...]
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- 2024
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34. Biallelic Cys141Tyr variant of SEL1L is associated with neurodevelopmental disorders, agammaglobulinemia, and premature death
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Weis, Denisa, Lin, Liangguang L., Wang, Huilun H., Li, Zexin Jason, Kusikova, Katarina, Ciznar, Peter, Wolf, Hermann M., Leiss-Piller, Alexander, Wang, Zhihong, Wei, Xiaoqiong, Weis, Serge, Skalicka, Katarina, Hrckova, Gabriela, Danisovic, Lubos, Soltysova, Andrea, Yang, Tingxuan T., Feichtinger, Rene Gunther, Mayr, Johannes A., and Qi, Ling
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Ubiquitin-proteasome system -- Physiological aspects -- Health aspects ,Agammaglobulinemia -- Genetic aspects -- Development and progression ,Endoplasmic reticulum -- Physiological aspects -- Health aspects ,Genetic variation -- Physiological aspects -- Health aspects - Abstract
Suppressor of lin-12-like-HMG-CoA reductase degradation 1 (SEL1L-HRD1) ER- associated degradation (ERAD) plays a critical role in many physiological processes in mice, including immunity, water homeostasis, and energy metabolism; however, its relevance and importance in humans remain unclear, as no disease variant has been identified. Here, we report a biallelic SEL1L variant (p. Cys141Tyr) in 5 patients from a consanguineous Slovakian family. These patients presented with not only ERAD-associated neurodevelopmental disorders with onset in infancy (ENDI) syndromes, but infantile- onset agammaglobulinemia with no mature B cells, resulting in frequent infections and early death. This variant disrupted the formation of a disulfide bond in the luminal fibronectin II domain of SEL1L, largely abolishing the function of the SEL1L-HRD1 ERAD complex in part via proteasomal-mediated self destruction by HRD1. This study reports a disease entity termed ENDI-agammaglobulinemia (ENDI-A) syndrome and establishes an inverse correlation between SEL1L-HRD1 ERAD functionality and disease severity in humans., Introduction ER-associated degradation (ERAD) is the key cellular quality-control mechanism underlying the clearance of misfolded proteins from the ER, thereby generating a conducive environment for protein folding, maturation, and maintaining [...]
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- 2024
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35. Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders
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Wang, Huilun H., Lin, Liangguang L., Li, Zexin J., Wei, Xiaoqiong, Askander, Omar, Cappuccio, Gerarda, Hashem, Mais O., Huber, Laurence, Munnich, Arnold, Alqahtani, Mashael, Pang, Qi, Burmeister, Margit, Lu, You, Poirier, Karine, Besmond, Claude, Sun, Shengyi, Brunetti-Pierri, Nicola, Alkuraya, Fowzan S., and Qi, Ling
- Subjects
Child development deviations -- Development and progression -- Genetic aspects -- Causes of ,Nervous system diseases -- Development and progression -- Genetic aspects -- Causes of ,Endoplasmic reticulum -- Health aspects ,Neurological research ,Genetic variation -- Research ,Developmental disabilities -- Development and progression -- Genetic aspects -- Causes of - Abstract
Recent studies using cell type-specific knockout mouse models have improved our understanding of the pathophysiological relevance of suppressor of lin-12-like-HMG-CoA reductase degradation 1 (SEL1L- HRD1) endoplasmic reticulum- associated (ER-associated) degradation (ERAD); however, its importance in humans remains unclear, as no disease variant has been identified. Here, we report the identification of 3 biallelic missense variants of SEL1L and HRD1 (or SYVN1) in 6 children from 3 independent families presenting with developmental delay, intellectual disability, microcephaly, facial dysmorphisms, hypotonia, and/or ataxia. These SEL1L (p.Gly585Asp, p.Met528Arg) and HRD1 (p.Pro398Leu) variants were hypomorphic and impaired ERAD function at distinct steps of ERAD, including substrate recruitment (SEL1L p.Gly585Asp), SEL1L-HRD1 complex formation (SEL1L p.Met528Arg), and HRD1 activity (HRD1 p.Pro398Leu). Our study not only provides insights into the structure-function relationship of SEL1L-HRD1 ERAD, but also establishes the importance of SEL1L-HRD1 ERAD in humans., Introduction Nascent membrane or secretory proteins are synthesized and folded in the endoplasmic reticulum (ER), which is prone to misfolding. Such misfolding may have pathogenic consequences if not cleared effectively [...]
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- 2024
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36. Galectin-3 aggravates microglial activation and tau transmission in tauopathy
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Siew, Jian Jing, Chen, Hui-Mei, Chiu, Feng-Lan, Lee, Chia-Wei, Chang, Yao- Ming, Chen, Hung-Lin, Nguyen, Thi Ngoc Anh, Liao, Hung-Ting, Liu, Mengyu, Hagar, Hsiao-Tien, Sun, Yung-Chen, Lai, Hsing- Lin, Kuo, Min-Hao, Blum, David, Buee, Luc, Jin, Lee-Way, Chen, Shih-Yu, Ko, Tai-Ming, Huang, Jie-Rong, Kuo, Hung-Chih, Liu, Fu-Tong, and Chern, Yijuang
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Tau proteins -- Health aspects ,Alzheimer's disease -- Development and progression ,Cellular control mechanisms -- Health aspects ,Lectins -- Health aspects - Abstract
Alzheimer's disease is characterized by the accumulation of amyloid-[beta] plaques, aggregation of hyperphosphorylated tau (pTau), and microglia activation. Galectin-3 (Gal3) is a [beta]-galactoside- binding protein that has been implicated in amyloid pathology. Its role in tauopathy remains enigmatic. Here, we showed that Gal3 was upregulated in the microglia of humans and mice with tauopathy. pTau triggered the release of Gal3 from human induced pluripotent stem cell-derived microglia in both its free and extracellular vesicular-associated (EV-associated) forms. Both forms of Gal3 increased the accumulation of pathogenic tau in recipient cells. Binding of Gal3 to pTau greatly enhanced tau fibrillation. Besides Gal3, pTau was sorted into EVs for transmission. Moreover, pTau markedly enhanced the number of EVs released by iMGL in a Gal3-dependent manner, suggesting a role of Gal3 in biogenesis of EVs. Single-cell RNA-Seq analysis of the hippocampus of a mouse model of tauopathy (THY-Tau22) revealed a group of pathogenic tau-evoked, Gal3- associated microglia with altered cellular machineries implicated in neurodegeneration, including enhanced immune and inflammatory responses. Genetic removal of Gal3 in THY-Tau22 mice suppressed microglia activation, reduced the level of pTau and synaptic loss in neurons, and rescued memory impairment. Collectively, Gal3 is a potential therapeutic target for tauopathy., Introduction Neuronal inclusions consisting of the microtubule-associated protein tau are found in various neurodegenerative diseases known as tauopathies. Alzheimer's disease (AD) is the most prevalent tauopathy and is characterized by [...]
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- 2024
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37. The nociceptin/orphanin FQ receptor partial agonist sunobinop promotes non-REM sleep in rodents and patients with insomnia
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Whiteside, Garth T., Kyle, Donald J., Kapil, Ram P., Cipriano, Alessandra, He, Ellie, Zhou, Mingyan, Shet, Manjunath S., Hummel, Michele, Knappenberger, Terri, Fukumura, Kazuya, Matsuo, Yoshiyuki, Uehira, Masahiro, Hiroyama, Shuichi, Takai, Nozomi, Willsie, Sandra K., and Harris, Stephen C.
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Health care industry - Abstract
To the Editor: Insomnia is a common disorder and public health burden. Frequently prescribed hypnotics enhance [gamma]-aminobutyric acid or block orexin signaling. With few pharmacological modalities and limitations associated with [...]
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- 2024
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38. Thrombotic microangiopathy following systemic AAV administration is dependent on anti-capsid antibodies
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Salabarria, Stephanie M., Corti, Manuela, Coleman, Kirsten E., Wichman, Megan B., Berthy, Julie A., D'Souza, Precilla, Tifft, Cynthia J., Herzog, Roland W., Elder, Melissa E., Shoemaker, Lawrence R., Leon-Astudillo, Carmen, Tavakkoli, Fatemeh, Kirn, David H., Schwartz, Jonathan D., and Byrne, Barry J.
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Gene therapy -- Patient outcomes ,Immune response -- Health aspects ,Adenoviruses -- Identification and classification -- Control ,Thrombocytopenic purpura -- Diagnosis -- Care and treatment -- Genetic aspects ,Health care industry ,Control ,Identification and classification ,Diagnosis ,Care and treatment ,Genetic aspects ,Patient outcomes ,Health aspects - Abstract
BACKGROUND. Systemic administration of adeno-associated virus (AAV) can trigger life-threatening inflammatory responses, including thrombotic microangiopathy (TMA), acute kidney injury due to atypical hemolytic uremic syndrome- like complement activation, immune-mediated myocardial inflammation, and hepatic toxicity. METHODS. We describe the kinetics of immune activation following systemic AAV serotype 9 (AAV9) administration in 38 individuals following 2 distinct prophylactic immunomodulation regimens. Group 1 received corticosteroids and Group 2 received rituximab plus sirolimus in addition to steroids to prevent anti-AAV antibody formation. RESULTS. Group 1 participants had a rapid increase in immunoglobulin M (IgM) and IgG. Increase in D-dimer, decline in platelet count, and complement activation are indicative of TMA. All Group 1 participants demonstrated activation of both classical and alternative complement pathways, as indicated by depleted C4 and elevated soluble C5b-9, Ba, and Bb antigens. Group 2 patients did not have a significant change in IgM or IgG and had minimal complement activation. CONCLUSIONS. This study demonstrates that TMA in the setting of AAV gene therapy is antibody dependent (classical pathway) and amplified by the alternative complement pathway. Critical time points and interventions are identified to allow for management of immune-mediated events that impact the safety and efficacy of systemic gene therapy., Introduction Adeno-associated virus-mediated (AAV-mediated) gene therapy has emerged as a promising treatment approach for a spectrum of monogenic genetic disorders (1-4). With the approval of the first recombinant AAV vector-based [...]
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- 2024
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39. Building reproducible bridges to cross the 'valley of death'
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Errington, Timothy M.
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Drug discovery -- Methods ,Clinical trials -- Laws, regulations and rules ,Evidence-based medicine -- Methods ,Health care industry ,Government regulation ,Methods ,Laws, regulations and rules - Abstract
Research is cumulative. New investigations build on, challenge, or qualify claims based on prior evidence. When we have limited evidence, it is normal that some explanations are wrong and need [...]
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- 2024
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40. In vivo gene editing of CAMKIID: out with the bad and in with the good
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Smith, John E., III and Granzier, Henk
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Heart diseases -- Diagnosis -- Care and treatment -- Genetic aspects ,Protein kinases -- Analysis ,Health care industry ,Diagnosis ,Care and treatment ,Analysis ,Genetic aspects ,Methods - Abstract
The ability to change an organism's DNA through gene editing is of great importance for the prevention and treatment of genetic and acquired diseases. Rapid progress has been made during the last decade due to the discovery and refinement of CRISPR/Cas9 as an accurate, fast, and reliable genome editing technique. In this issue of the JCI, Lebek et al. present the culmination from a line of work in the Olson laboratory focused on in vivo gene editing of CAMK2D. The paper presents a combined state-of-the-art gene therapy approach that demonstrates how gene therapy can yield cardioprotection in a mouse model and takes notable steps toward potential applicability in patients., CAMK2D The CAMK2D gene encodes calcium/ calmodulin dependent protein kinase II delta (CaMKII[delta]), one of the major CaMKII isoforms and the predominant isoform in the heart. CaMKII[delta] contributes to the [...]
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- 2024
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41. Solid organ transplantation: solid but not yet spectacular
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Turka, Laurence A.
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Transplantation of organs, tissues, etc. -- Practice ,Immunosuppression -- Patient outcomes ,Health care industry ,Practice ,Patient outcomes - Abstract
As a former editor-in-chief of this journal, it is a particular pleasure to provide a contribution in recognition of the JCI's 100th anniversary. My invitation asked me to provide a [...]
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- 2024
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42. PP2A inhibition causes synthetic lethality in BRCA2-mutated prostate cancer models via spindle assembly checkpoint reactivation
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Wang, Jian, Chen, Yuke, Li, Shiwei, Liu, Wanchang, Zhou, Xiao Albert, Luo, Yefei, Xu, Zhanzhan, Xiong, Yundong, Cheng, Kaiqi, Ruan, Mingjian, Yu, Wei, Li, Xiaoman, Wang, Weibin, and Wang, Jiadong
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Gene mutations -- Health aspects ,DNA replication -- Health aspects ,Prostate cancer -- Diagnosis -- Care and treatment -- Genetic aspects ,Tumor suppressor genes -- Analysis ,Health care industry ,Diagnosis ,Care and treatment ,Analysis ,Genetic aspects ,Health aspects - Abstract
Mutations in the BRCA2 tumor suppressor gene have been associated with an increased risk of developing prostate cancer. One of the paradoxes concerning BRCA2 is the fact that its inactivation affects genetic stability and is deleterious for cellular and organismal survival, while BRCA2-mutated cancer cells adapt to this detriment and malignantly proliferate. Therapeutic strategies for tumors arising from BRCA2 mutations may be discovered by understanding these adaptive mechanisms. In this study, we conducted forward genetic synthetic viability screenings in Caenorhabditis elegans brc-2 (Cebrc-2) mutants and found that Ceubxn-2 inactivation rescued the viability of Cebrc-2 mutants. Moreover, loss of NSFL1C, the mammalian ortholog of CeUBXN-2, suppressed the spindle assembly checkpoint (SAC) activation and promoted the survival of BRCA2- deficient cells. Mechanistically, NSFL1C recruited USP9X to inhibit the polyubiquitination of AURKB and reduce the removal of AURKB from the centromeres by VCP, which is essential for SAC activation. SAC inactivation is common in BRCA2-deficient prostate cancer patients, but PP2A inhibitors could reactivate the SAC and achieve BRCA2- deficient prostate tumor synthetic lethality. Our research reveals the survival adaptation mechanism of BRCA2-deficient prostate tumor cells and provides different angles for exploring synthetic lethal inhibitors in addition to targeting DNA damage repair pathways., Introduction Germline mutations of BRCA2 predispose humans to prostate, breast, ovarian, and pancreatic cancers (1, 2). As a tumor suppressor protein, BRCA2 is essential for stabilizing stalled replication forks (2, [...]
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- 2024
- Full Text
- View/download PDF
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