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The interaction of Synapsin 2a and Synaptogyrin-3 regulates fear extinction in mice

Authors :
Shen, Xi-Ya
Zhang, Juan
Huang, He-Zhou
Li, Shao-Dan
Zhou, Ling
Wu, Shi-Ping
Tang, Cheng
Huang, Xian
Liu, Zhi-Qiang
Guo, Zi-Yuan
Li, Xiang
Man, Heng-Ye
Lu, You-Ming
Zhu, Ling-Qiang
Liu, Dan
Source :
Journal of Clinical Investigation. February 15, 2024, Vol. 134 Issue 4
Publication Year :
2024

Abstract

The mechanisms behind a lack of efficient fear extinction in some individuals are unclear. Here, by employing a principal components analysis-based approach, we differentiated the mice into extinction- resistant and susceptible groups. We determined that elevated synapsin 2a (Syn2a) in the infralimbic cortex (IL) to basolateral amygdala (BLA) circuit disrupted presynaptic orchestration, leading to an excitatory/inhibitory imbalance in the BLA region and causing extinction resistance. Overexpression or silencing of Syn2a levels in IL neurons replicated or alleviated behavioral, electrophysiological, and biochemical phenotypes in resistant mice. We further identified that the proline-rich domain H in the C-terminus of Syn2a was indispensable for the interaction with synaptogyrin-3 (Syngr3) and demonstrated that disrupting this interaction restored extinction impairments. Molecular docking revealed that ritonavir, an FDA- approved HIV drug, could disrupt Syn2a- Syngr3 binding and rescue fear extinction behavior in Syn2a-elevated mice. In summary, the aberrant elevation of Syn2a expression and its interaction with Syngr3 at the presynaptic site were crucial in fear extinction resistance, suggesting a potential therapeutic avenue for related disorders.<br />Introduction Posttraumatic stress disorder (PTSD) is a debilitating neuropsychiatric disorder (1) with complicated psychological and neurobiological mechanisms involving varied brain circuits mediating stress and fear responses (2-4), as well as [...]

Details

Language :
English
ISSN :
00219738
Volume :
134
Issue :
4
Database :
Gale General OneFile
Journal :
Journal of Clinical Investigation
Publication Type :
Academic Journal
Accession number :
edsgcl.786063953
Full Text :
https://doi.org/10.1172/JCI172802