Search

Theranostics is a concept that integrated imaging and therapy. As an emerging field, it embraces multiple techniques to arrive at an individualized treatment purpose. Indocyanine green (ICG) is a near infrared dye that has been approved by Food and Drug Administration (FDA) in USA for the use in indicator-dilution studies in humans. ICG nanoparticles (NPs) have attracted much attention for its potential applications in cancer theranostics. This review focuses on the preparation, application of ICG NPs for in vivo imaging (fluorescent imaging and photoacoustic imaging) and therapeutics (photothermal therapy, photodynamic therapy and photoacoustic therapy), and future directions based on recent developments in these areas. It is hoped that this review might provide new impetus to understand ICG NPs for cancer theranostics. [ABSTRACT FROM AUTHOR]

Porcine circovirus type 2 (PCV2) is associated with many diseases especially postweaning multisystemic wasting syndrome (PMWS), which has brought huge economic loss to the swine industry worldwide. Viral detection will pave the way for this disease prevention. Herein, we developed a facile, rapid and ultrasensitive method for detection of PCV2 based on gold(iii) enhanced chemiluminescence immunoassay (CLIA). The gold(iii), dissolved from the gold nanoparticle–monoclonal antibody conjugate, served as an analyte for the indirect measurement of PCV2. Under the optimal conditions, the detection limit for the detection of PCV2 was 1.71 × 103copies mL−1. Moreover, the CLIA signal can be further enhanced by using hydroxylamine-amplified gold nanoparticles, and the limit of detection was as low as 2.67 × 102copies mL−1. Compared with conventional polymerase chain reaction (PCR), the proposed method has good sensitivity and reliability in analysis of 36 serum samples, and showed great potential in virus assays. [ABSTRACT FROM AUTHOR]

In this paper, we report a new homogeneous assay for rapid, highly selective and sensitive detection of Hg2+in aqueous solution based on the induced photoluminescence (PL) quenching of BSA-modified gold nanoclusters (BSA-Au NCs). There was a linear correlation between the expression (I0− I)/I0and the concentrations of Hg2+over the ranges of 0.4–43.2 μM, and the corresponding limit of detection (LOD) was 80 nM. The relative standard deviation of 5 replicate measurements was 1.4% for 1.0 × 10−5mol L−1Hg2+. Moreover, this method has excellent selectivity over metal ions and anions. The feasibility of the BSA-Au NCs sensor for Hg2+in different aqueous samples was demonstrated with satisfactory results. Moreover, the possible sensing Hg2+mechanism was also discussed. [ABSTRACT FROM AUTHOR]

A method has been developed for the rapid determination of calf thymus (ct) DNA that is based on the photoinduced electron transfer (PET) that occurs between CdTe quantum dots and the ruthenium(II)tris-bipyridyl complex. The latter quenches the photoluminescence (PL) of the quantum dots through PET. The Stern-Volmer quenching constant is 2,500 L mol−1. The intensity of the PL the system is recovered in the presence of ct DNA, and relative recovered PL intensity is linearly proportional to the concentration of ct-DNA. The dynamic range is from 17 µM to 1.5 mM of DNA, and the detection limit (at S/ N = 3) is 5.7 µM. The relative standard deviation (at 0.5 mM of ct-DNA) is 4.1% ( n = 11). A possible reaction mechanism is discussed. [ABSTRACT FROM AUTHOR]

Selective determination of Ag(I) ion was accomplished based on the red-shift of the emission band of quantum dots (QDs). Under optimal conditions, a linear relationship does exist between the red-shift of the emission and the concentration of Ag(I) in the range from 1.0 × 10−7 to 1.5 × 10−5 mol L−1, with a detection limit of 5.0 × 10−8 mol L−1. The method has been successfully applied to the determination of Ag(I) ion in water samples. The possible reaction mechanism was investigated by ultraviolet–visible absorption, fluorescence, Raman spectroscopies and by high resolution transmission electron microscopy. The results suggest that the red-shift in emission be attributed to the stabilization of a charge-transfer state, but not due to the aggregation induced by AgI(I) ion. [ABSTRACT FROM AUTHOR]

Here we report a indirect fluorescence immunoassay for high-throughput screening of APP based on the fluorescence quenching of quantum dots by gold(III), which were dissolved from gold nanoparticles-Rabbit anti-Pig IgG conjugate, and further utilize this system to detect APP in pig serum with high sensitivity and specificity. [ABSTRACT FROM AUTHOR]

Here we report a indirect fluorescence immunoassay for high-throughput screening of APP based on the fluorescence quenching of quantum dots by gold(iii), which were dissolved from gold nanoparticles-Rabbit anti-Pig IgG conjugate, and further utilize this system to detect APP in pig serum with high sensitivity and specificity. [ABSTRACT FROM AUTHOR]