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Ultrasensitive detection of porcine circovirus type 2 using gold(iii) enhanced chemiluminescence immunoassay.
- Source :
- Analyst; Jul2010, Vol. 135 Issue 7, p1680-1685, 6p
- Publication Year :
- 2010
-
Abstract
- Porcine circovirus type 2 (PCV2) is associated with many diseases especially postweaning multisystemic wasting syndrome (PMWS), which has brought huge economic loss to the swine industry worldwide. Viral detection will pave the way for this disease prevention. Herein, we developed a facile, rapid and ultrasensitive method for detection of PCV2 based on gold(iii) enhanced chemiluminescence immunoassay (CLIA). The gold(iii), dissolved from the gold nanoparticleâmonoclonal antibody conjugate, served as an analyte for the indirect measurement of PCV2. Under the optimal conditions, the detection limit for the detection of PCV2 was 1.71 Ã 103copies mLâ1. Moreover, the CLIA signal can be further enhanced by using hydroxylamine-amplified gold nanoparticles, and the limit of detection was as low as 2.67 Ã 102copies mLâ1. Compared with conventional polymerase chain reaction (PCR), the proposed method has good sensitivity and reliability in analysis of 36 serum samples, and showed great potential in virus assays. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 135
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 51807145
- Full Text :
- https://doi.org/10.1039/c0an00025f