20 results on '"Sanderson-Smith, Martina L."'
Search Results
2. An optimised GAS-pharyngeal cell biofilm model.
- Author
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Vyas, Heema K. N., McArthur, Jason D., and Sanderson-Smith, Martina L.
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STREPTOCOCCUS ,BIOFILMS ,VIRULENCE of bacteria ,BIOMASS ,BACTERIAL cells - Abstract
Group A Streptococcus (GAS) causes 700 million infections and accounts for half a million deaths per year. Biofilm formation has been implicated in both pharyngeal and dermal GAS infections. In vitro, plate-based assays have shown that several GAS M-types form biofilms, and multiple GAS virulence factors have been linked to biofilm formation. Although the contributions of these plate-based studies have been valuable, most have failed to mimic the host environment, with many studies utilising abiotic surfaces. GAS is a human specific pathogen, and colonisation and subsequent biofilm formation is likely facilitated by distinct interactions with host tissue surfaces. As such, a host cell-GAS model has been optimised to support and grow GAS biofilms of a variety of GAS M-types. Improvements and adjustments to the crystal violet biofilm biomass assay have also been tailored to reproducibly detect delicate GAS biofilms. We propose 72 h as an optimal growth period for yielding detectable biofilm biomass. GAS biofilms formed are robust and durable, and can be reproducibly assessed via staining/washing intensive assays such as crystal violet with the aid of methanol fixation prior to staining. Lastly, SEM imaging of GAS biofilms formed by this model revealed GAS cocci chains arranged into three-dimensional aggregated structures with EPS matrix material. Taken together, we outline an efficacious GAS biofilm pharyngeal cell model that can support long-term GAS biofilm formation, with biofilms formed closely resembling those seen in vivo. [ABSTRACT FROM AUTHOR]
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- 2021
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3. Streptococcus pyogenes M1T1 Variants Induce an Inflammatory Neutrophil Phenotype Including Activation of Inflammatory Caspases.
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Williams, Jonathan G., Ly, Diane, Geraghty, Nicholas J., McArthur, Jason D., Vyas, Heema K. N., Gorman, Jody, Tsatsaronis, James A., Sluyter, Ronald, and Sanderson-Smith, Martina L.
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STREPTOCOCCUS pyogenes ,CASPASES ,PHENOTYPES ,REACTIVE oxygen species - Abstract
Invasive infections due to group A Streptococcus (GAS) advance rapidly causing tissue degradation and unregulated inflammation. Neutrophils are the primary immune cells that respond to GAS. The neutrophil response to GAS was characterised in response to two M1T1 isolates; 5448 and animal passaged variant 5448AP. Co-incubation of neutrophils with 5448AP resulted in proliferation of GAS and lowered the production of reactive oxygen species when compared with 5448. Infection with both strains invoked neutrophil death, however apoptosis was reduced in response to 5448AP. Both strains induced neutrophil caspase-1 and caspase-4 expression in vitro , with inflammatory caspase activation detected in vitro and in vivo. GAS infections involving strains such as 5448AP that promote an inflammatory neutrophil phenotype may contribute to increased inflammation yet ineffective bacterial eradication, contributing to the severity of invasive GAS infections. [ABSTRACT FROM AUTHOR]
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- 2021
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4. Human glycan expression patterns influence Group A streptococcal colonization of epithelial cells.
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De Oliveira, David M. P., Everest-Dass, Arun, Hartley-Tassell, Lauren, Day, Christopher J., Indraratna, Anuk, Brouwer, Stephan, Cleary, Ailish, Kautto, Liisa, Gorman, Jody, Packer, Nicolle H., Jennings, Michael P., Walker, Mark J., and Sanderson-Smith, Martina L.
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- 2019
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5. Immune Cross-Opsonization Within emm Clusters Following Group A Streptococcus Skin Infection: Broadening the Scope of Type-Specific Immunity.
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Frost, Hannah R., Laho, Delphine, Sanderson-Smith, Martina L., Licciardi, Paul, Donath, Susan, Curtis, Nigel, Kado, Joseph, Dale, James B., Steer, Andrew C., and Smeesters, Pierre R.
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ANTIGEN-antibody reactions ,COMMUNICABLE diseases ,ENZYME-linked immunosorbent assay ,PEPTIDES ,SERODIAGNOSIS ,SKIN diseases ,STREPTOCOCCAL diseases ,VACCINES - Abstract
Background. Group A Streptococcus (GAS) skin infections are particularly prevalent in developing nations. The GAS M protein, by which strains are differentiated into >220 different emm types, is immunogenic and elicits protective antibodies. A major obstacle for vaccine development has been the traditional understanding that immunity following infection is restricted to a single emm type. However, recent evidence has led to the hypothesis of immune cross-reactivity between emm types. Methods. We investigated the human serological response to GAS impetigo in Fijian schoolchildren, focusing on 3 major emm clusters (E4, E6, and D4). Pre- and postinfection sera were assayed by enzyme-linked immunosorbent assay with N-terminal M peptides and bactericidal assays using the infecting-type strain, emm cluster-related strains, and nonrelated strains. Results. Twenty of the 53 paired sera demonstrated a =4-fold increase in antibody titer against the infecting type. When tested against all cluster-related M peptides, we found that 9 of 17 (53%) paired sera had a =4-fold increase in antibody titer to cluster-related strains as well. When grouped by cluster, the mean change to cluster-related emm types in E4 and E6 was >4-fold (5.9-fold and 19.5-fold, respectively) but for D4 was 3.8-fold. The 17 paired sera were tested in bactericidal assays against selected cluster-related and nonrelated strains. While the responses were highly variable, numerous instances of cross-reactive killing were observed. Conclusions. These data demonstrate that M type-specific and cross-reactive immune responses occur following skin infection. The cross-reactive immune responses frequently align with emm clusters, raising new opportunities to design multivalent vaccines with broad coverage. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Group A Streptococcus Modulates Host Inflammation by Manipulating Polymorphonuclear Leukocyte Cell Death Responses.
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Tsatsaronis, James a., Ly, Diane, Pupovac, aleta, Goldmann, Oliver, Rohde, Manfred, Taylor, Jude M., Walker, Mark J., Medina, Eva, and Sanderson-Smith, Martina L.
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- 2015
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7. Molecular Markers for the Study of Streptococcal Epidemiology.
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McMillan, David J., Sanderson-Smith, Martina L., Smeesters, Pierre Robert, and Sriprakash, Kadaba S.
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- 2013
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8. Host Responses to Group A Streptococcus: Cell Death and Inflammation.
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Tsatsaronis, James A., Walker, Mark J., and Sanderson-Smith, Martina L.
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STREPTOCOCCUS ,INFLAMMATION ,CELL death ,LEUCOCYTES ,EPITHELIAL cells ,APOPTOSIS - Abstract
Infections caused by group A Streptococcus (GAS) are characterized by robust inflammatory responses and can rapidly lead to life-threatening disease manifestations. However, host mechanisms that respond to GAS, which may influence disease pathology, are understudied. Recent works indicate that GAS infection is recognized by multiple extracellular and intracellular receptors and activates cell signalling via discrete pathways. Host leukocyte receptor binding to GAS-derived products mediates release of inflammatory mediators associated with severe GAS disease. GAS induces divergent phagocyte programmed cell death responses and has inflammatory implications. Epithelial cell apoptotic and autophagic components are mobilized by GAS infection, but can be subverted to ensure bacterial survival. Examination of host interactions with GAS and consequences of GAS infection in the context of cellular receptors responsible for GAS recognition, inflammatory mediator responses, and cell death mechanisms, highlights potential avenues for diagnostic and therapeutic intervention. Understanding the molecular and cellular basis of host symptoms during severe GAS disease will assist the development of improved treatment regimens for this formidable pathogen. [ABSTRACT FROM AUTHOR]
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- 2014
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9. Site-restricted plasminogen activation mediated by group A streptococcal streptokinase variants.
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COOK, Simon M., SKORA, Amanda, WALKER, Mark J., SANDERSON-SMITH, Martina L., and MCARTHUR, Jason D.
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STREPTOCOCCUS pyogenes ,PLASMINOGEN activators ,STREPTOKINASE ,THROMBOLYTIC therapy ,FIBRINOGEN ,PLASMINOGEN - Abstract
SK (streptokinase) is a secreted plasminogen activator and virulence factor of GAS (group A Streptococcus). Among GAS isolates, SK gene sequences are polymorphic and are grouped into two sequence clusters (cluster type-1 and cluster type- 2) with cluster type-2 being further classified into subclusters (type-2a and type-2b). In the present study, we examined the role of bacterial and host-derived cofactors in SK-mediated plasminogen activation. All SK variants, apart from type-2b, can form an activator complex with Glu-Plg (Glu-plasminogen). Specific ligand-binding-induced conformational changes in Glu- Plg mediated by fibrinogen, PAM (plasminogen-binding group A streptococcal M protein), fibrinogen fragment D or fibrin, were required for type-2b SK to form a functional activator complex with Glu-Plg. In contrast with type-1 and type-2a SK, type-2b SK activator complexes were inhibited by a2-antiplasmin unless bound to fibrin or to the GAS cell-surface via PAMin combination with fibrinogen. Taken together, these data suggest that type-2b SK plasminogen activation may be restricted to specific microenvironments within the host such as fibrin deposits or the bacterial cell surface through the action of a2-antiplasmin. We conclude that phenotypic SK variation functionally underpins a pathogenic mechanismwhereby SK variants differentially focus plasminogen activation, leading to specific niche adaption within the host. [ABSTRACT FROM AUTHOR]
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- 2014
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10. Plasmin(ogen) Acquisition by Group A Streptococcus Protects against C3b-Mediated Neutrophil Killing.
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Ly, Diane, Taylor, Jude M., Tsatsaronis, James a., Monteleone, Mercedes M., Skora, amanda S., Donald, Cortny a., Maddocks, Tracy, Nizet, Victor, West, Nicholas P., Ranson, Marie, Walker, Mark J., Mcarthur, Jason D., and Sanderson-Smith, Martina L.
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- 2014
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11. Streptococcal collagen-like protein A and general stress protein 24 are immunomodulating virulence factors of group A Streptococcus.
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Tsatsaronis, James A., Hollands, Andrew, Cole, Jason N., Maamary, Peter G., Gillen, Christine M., Ben Zakour, Nouri L., Kotb, Malak, Nizet, Victor, Beatson, Scott A., Walker, Mark J., and Sanderson-Smith, Martina L.
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SYSTEMS biology ,NATURAL immunity ,DEVELOPING countries ,STREPTOCOCCUS ,SEROTYPES - Abstract
In Western countries, invasive infections caused by M1T1 serotype group A Streptococcus (GAS) are epidemiologically linked to mutations in the control of virulence regulatory 2-component operon (covRS). In indigenous communities and developing countries, severe GAS disease is associated with genetically diverse non-M1T1 GAS serotypes. Hypervirulent M1T1 covRS mutant strains arise through selection by human polymorphonuclear cells for increased expression of GAS virulence factors such as the DNase Sda1, which promotes neutrophil resistance. The GAS bacteremia isolate NS88.2 (emm 98.1) is a covS mutant that exhibits a hypervirulent phenotype and neutrophil resistance yet lacks the phage-encoded Sda1. Here, we have employed a comprehensive systems biology (genomic, transcriptomic, and proteomic) approach to identify NS88.2 virulence determinants that enhance neutrophil resistance in the non-M1T1 GAS genetic background. Using this approach, we have identified streptococcal collagen-like protein A and general stress protein 24 proteins as NS88.2 determinants that contribute to survival in whole blood and neutrophil resistance in non-M1T1 GAS. This study has revealed new factors that contribute to GAS pathogenicity that may play important roles in resisting innate immune defenses and the development of human invasive infections. [ABSTRACT FROM AUTHOR]
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- 2013
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12. A Key Role for the Urokinase Plasminogen Activator (uPA) in Invasive Group A Streptococcal Infection.
- Author
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Sanderson-Smith, Martina L., Zhang, Yueling, Ly, Diane, Donahue, Deborah, Hollands, Andrew, Nizet, Victor, Ranson, Marie, Ploplis, Victoria A., Walker, Mark J., and Castellino, Francis J.
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UROKINASE ,PLASMINOGEN activators ,STREPTOCOCCAL diseases ,BACTERIAL diseases ,PATHOGENIC microorganisms - Abstract
Recruitment of the serine protease plasmin is central to the pathogenesis of many bacterial species, including Group A streptococcus (GAS), a leading cause of morbidity and mortality globally. A key process in invasive GAS disease is the ability to accumulate plasmin at the cell surface, however the role of host activators of plasminogen in this process is poorly understood. Here, we demonstrate for the first time that the urokinase-type plasminogen activator (uPA) contributes to plasmin recruitment and subsequent invasive disease initiation in vivo. In the absence of a source of host plasminogen activators, streptokinase (Ska) was required to facilitate cell surface plasmin acquisition by GAS. However, in the absence of Ska, host activators were sufficient to promote cell surface plasmin acquisition by GAS strain 5448 during incubation with plasminogen or human plasma. Furthermore, GAS were able mediate a significant increase in the activation of zymogen pro-uPA in human plasma. In order to assess the contribution of uPA to invasive GAS disease, a previously undescribed transgenic mouse model of infection was employed. Both C57/black 6J, and AlbPLG1 mice expressing the human plasminogen transgene, were significantly more susceptible to invasive GAS disease than uPA−/− mice. The observed decrease in virulence in uPA−/−mice was found to correlate directly with a decrease in bacterial dissemination and reduced cell surface plasmin accumulation by GAS. These findings have significant implications for our understanding of GAS pathogenesis, and research aimed at therapeutic targeting of plasminogen activation in invasive bacterial infections. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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13. Bacterial Plasminogen Receptors: Mediators of a Multifaceted Relationship.
- Author
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Sanderson-Smith, Martina L., De Oliveira, David M. P., Ranson, Marie, and McArthur, Jason D.
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Multiple species of bacteria are able to sequester the host zymogen plasminogen to the cell surface. Once localised to the bacterial surface, plasminogen can act as a cofactor in adhesion, or, following activation to plasmin, provide a source of potent proteolytic activity. Numerous bacterial plasminogen receptors have been identified, and the mechanisms by which they interact with plasminogen are diverse. Here we provide an overview of bacterial plasminogen receptors and discuss the diverse role bacterial plasminogen acquisition plays in the relationship between bacteria and the host. [ABSTRACT FROM AUTHOR]
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- 2012
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14. Bacterial Plasminogen Receptors: Mediators of a Multifaceted Relationship.
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Sanderson-Smith, Martina L., De Oliveira, David M. P., Ranson, Marie, and McArthur, Jason D.
- Subjects
FIBRINOLYTIC agents ,CELL receptors ,METABOLISM - Abstract
Multiple species of bacteria are able to sequester the host zymogen plasminogen to the cell surface. Once localised to the bacterial surface, plasminogen can act as a cofactor in adhesion, or, following activation to plasmin, provide a source of potent proteolytic activity. Numerous bacterial plasminogen receptors have been identified, and the mechanisms by which they interact with plasminogen are diverse. Here we provide an overview of bacterial plasminogen receptors and discuss the diverse role bacterial plasminogen acquisition plays in the relationship between bacteria and the host. [ABSTRACT FROM AUTHOR]
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- 2012
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15. Parameters Governing Invasive Disease Propensity of Non-M1 Serotype Group A Streptococci.
- Author
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Maamary, Peter G., Sanderson-Smith, Martina L., Aziz, Ramy K., Hollands, Andrew, Cole, Jason N., McKay, Fiona C., McArthur, Jason D., Kirk, Joshua K., Cork, Amanda J., Keefe, Rachael J., Kansal, Rita G., Sun, Hongmin, Taylor, William L., Chhatwal, Gursharan S., Ginsburg, David, Nizet, Victor, Kotb, Malak, and Walker, Mark J.
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- 2010
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16. Allelic variants of streptokinase from Streptococcus pyogenes display functional differences in plasminogen activation.
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McArthur, Jason D., McKay, Fiona C., Ramachandran, Vidiya, Shyam, Priya, Cork, Amanda J., Sanderson-Smith, Martina L., Cole, Jason N., Ringdahl, Ulrika, Sjöbring, Ulf, Ranson, Marie, and Walker, Mark J.
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STREPTOKINASE ,STREPTOCOCCUS pyogenes ,PLASMINOGEN activators ,FIBRINOGEN ,MICROBIAL virulence - Abstract
A common mammalian defense mechanism employed to prevent systemic dissemination of invasive bacteria involves occlusion of local microvasculature and encapsulation of bacteria within fibrin networks. Acquisition of plasmin activity at the bacterial cell surface circumvents this defense mechanism, allowing invasive disease initiation. To facilitate this process, S. pyogenes secretes streptokinase, a plasminogen-activating protein. Streptokinase polymorphism exhibited by S. pyogenes isolates is well characterized. However, the functional differences displayed by these variants and the biological significance of this variation has not been elucidated. Phylogenetic analysis of ska sequences from 28 S. pyogenes isolates revealed 2 main sequence clusters (clusters 1 and 2). All strains secreted streptokinase, as determined by Western blotting, and were capable of acquiring cell surface plasmin activity after incubation in human plasma. Whereas culture supernatants from strains containing cluster 1 ska alleles also displayed soluble plasminogen activation activity, supernatants from strains containing cluster 2 ska alleles did not. Furthermore, plasminogen activation activity in culture supernatants from strains containing cluster 2 ska alleles could only be detected when plasminogen was prebound with fibrinogen. This study indicates that variant streptokinase proteins secreted by S. pyogenes isolates display differing plasminogen activation characteristics and may therefore play distinct roles in disease pathogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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17. DNase Sda1 provides selection pressure for a switch to invasive group A streptococcal infection.
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Walker, Mark J., Hollands, Andrew, Sanderson-Smith, Martina L., Cole, Jason N., Kirk, Joshua K., Henningham, Anna, McArthur, Jason D., Dinkla, Katrin, Aziz, Ramy K., Kansal, Rita G., Simpson, Amelia J., Buchanan, John T., Chhatwal, Gursharan S., Kotb, Malak, and Nizet, Victor
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STREPTOCOCCAL diseases ,NECROTIZING fasciitis ,TOXIC shock syndrome ,FIBRINOLYTIC agents ,IMMUNE system ,CYSTEINE proteinases - Abstract
Most invasive bacterial infections are caused by species that more commonly colonize the human host with minimal symptoms. Although phenotypic or genetic correlates underlying a bacterium's shift to enhanced virulence have been studied, the in vivo selection pressures governing such shifts are poorly understood. The globally disseminated M1T1 clone of group A Streptococcus (GAS) is linked with the rare but life-threatening syndromes of necrotizing fasciitis and toxic shock syndrome. Mutations in the GAS control of virulence regulatory sensor kinase (covRS) operon are associated with severe invasive disease, abolishing expression of a broad-spectrum cysteine protease (SpeB) and allowing the recruitment and activation of host plasminogen on the bacterial surface. Here we describe how bacteriophage-encoded GAS DNase (Sda1), which facilitates the pathogen's escape from neutrophil extracellular traps, serves as a selective force for covRS mutation. The results provide a paradigm whereby natural selection exerted by the innate immune system generates hypervirulent bacterial variants with increased risk of systemic dissemination. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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18. Assessing the Role of Pharyngeal Cell Surface Glycans in Group A Streptococcus Biofilm Formation.
- Author
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Vyas, Heema K. N., Indraratna, Anuk D., Everest-Dass, Arun, Packer, Nicolle H., De Oliveira, David M. P., Ranson, Marie, McArthur, Jason D., Sanderson-Smith, Martina L., and Reffuveille, Fany
- Subjects
BIOFILMS ,CELL membranes ,GLYCANS ,GLYCAN structure ,STREPTOCOCCUS ,SIALIC acids - Abstract
Group A Streptococcus (GAS) causes 700 million infections and accounts for half a million deaths per year. Antibiotic treatment failure rates of 20–40% have been observed. The role host cell glycans play in GAS biofilm formation in the context of GAS pharyngitis and subsequent antibiotic treatment failure has not been previously investigated. GAS serotype M12 GAS biofilms were assessed for biofilm formation on Detroit 562 pharyngeal cell monolayers following enzymatic removal of all N-linked glycans from pharyngeal cells with PNGase F. Removal of N-linked glycans resulted in an increase in biofilm biomass compared to untreated controls. Further investigation into the removal of terminal mannose and sialic acid residues with α1-6 mannosidase and the broad specificity sialidase (Sialidase A) also found that biofilm biomass increased significantly when compared to untreated controls. Increases in biofilm biomass were associated with increased production of extracellular polymeric substances (EPS). Furthermore, it was found that M12 GAS biofilms grown on untreated pharyngeal monolayers exhibited a 2500-fold increase in penicillin tolerance compared to planktonic GAS. Pre-treatment of monolayers with exoglycosidases resulted in a further doubling of penicillin tolerance in resultant biofilms. Lastly, an additional eight GAS emm-types were assessed for biofilm formation in response to terminal mannose and sialic acid residue removal. As seen for M12, biofilm biomass on monolayers increased following removal of terminal mannose and sialic acid residues. Collectively, these data demonstrate that pharyngeal cell surface glycan structures directly impact GAS biofilm formation in a strain and glycan specific fashion. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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19. Prophage exotoxins enhance colonization fitness in epidemic scarlet fever-causing Streptococcus pyogenes.
- Author
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Brouwer, Stephan, Barnett, Timothy C., Ly, Diane, Kasper, Katherine J., De Oliveira, David M. P., Rivera-Hernandez, Tania, Cork, Amanda J., McIntyre, Liam, Jespersen, Magnus G., Richter, Johanna, Schulz, Benjamin L., Dougan, Gordon, Nizet, Victor, Yuen, Kwok-Yung, You, Yuanhai, McCormick, John K., Sanderson-Smith, Martina L., Davies, Mark R., and Walker, Mark J.
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STREPTOCOCCUS pyogenes ,SCARLATINA ,COLONIZATION ,STREPTOCOCCUS ,EXOTOXIN ,STREPTOCOCCUS pneumoniae ,PATHOLOGY ,WORLD health - Abstract
The re-emergence of scarlet fever poses a new global public health threat. The capacity of North-East Asian serotype M12 (emm12) Streptococcus pyogenes (group A Streptococcus, GAS) to cause scarlet fever has been linked epidemiologically to the presence of novel prophages, including prophage ΦHKU.vir encoding the secreted superantigens SSA and SpeC and the DNase Spd1. Here, we report the molecular characterization of ΦHKU.vir-encoded exotoxins. We demonstrate that streptolysin O (SLO)-induced glutathione efflux from host cellular stores is a previously unappreciated GAS virulence mechanism that promotes SSA release and activity, representing the first description of a thiol-activated bacterial superantigen. Spd1 is required for resistance to neutrophil killing. Investigating single, double and triple isogenic knockout mutants of the ΦHKU.vir-encoded exotoxins, we find that SpeC and Spd1 act synergistically to facilitate nasopharyngeal colonization in a mouse model. These results offer insight into the pathogenesis of scarlet fever-causing GAS mediated by prophage ΦHKU.vir exotoxins. The pathogenesis of Streptococcus pyogenes (GAS) causing scarlet fever has been associated with the presence of prophages, such as ΦHKU.vir, and their products. Here, the authors characterize the exotoxins SpeC and Spd1 of ΦHKU.vir and show these to act synergistically to facilitate nasopharyngeal colonization in mice. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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20. Trigger for group A streptococcal M1T1 invasive disease.
- Author
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Cole, Jason N., McArthur, Jason D., McKay, Fiona C., Sanderson-Smith, Martina L., Cork, Amanda J., Ranson, Marie, Rohde, Manfred, Itzek, Andreas, Hongmin Sun, Ginsburg, David, Kotb, Malak, Nizet, Victor, Chhatwal, G. S., and Walker, Mark J.
- Subjects
STREPTOCOCCAL diseases ,CYSTEINE proteinases ,MICROBIAL virulence ,PLASMINOGEN ,TRANSGENIC mice - Abstract
The article presents a study which investigated the trigger factor for group A streptococcal M1T1 invasive disease. The characterization and M1T1 5448 and cysteine protease SpeB is discussed. The progression of the virulence of the wild-type strain in humanized plasminogen transgenic mice is examined. A description of SpeB activity of 5448 in vivo isolates is provided.
- Published
- 2006
- Full Text
- View/download PDF
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