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Learning a new language is a process everyone undergoes at least once. However, studying the neural mechanisms behind first-time language learning is a challenging task. Here we aim to explore the functional alterations following learning Israeli Sign Language, a visuo-spatial rather than an auditory-based language. Specifically, we investigate how phonological, lexical, and sentence-level components of the language system differ in their neural representations. In this within-participant design, hearing individuals naïve to sign languages (n = 79) performed an fMRI task requiring the processing of different linguistic components, before and after attending an Israeli Sign Language course. A learning-induced increase in activation was detected in various brain regions in task contrasts related to all sign language linguistic components. Activation patterns while processing different linguistic components post-learning were spatially distinct, suggesting a unique neural representation for each component. Moreover, post-learning activation maps successfully predicted learning retention six months later, associating neural and performance measures. Sign language learning in naïve, hearing participants drives vast functional changes in the brain, as well as distinct neural representations of different linguistic components of sign language post-learning. [ABSTRACT FROM AUTHOR]

Purpose: Monoclonal antibodies have made an immense contribution to the treatment of various human diseases. We aimed at investigating an affordable treatment option for veterinary patients with corneal neovascularization by adding the preservative benzalkonium chloride (BAC) to bevacizumab (Avastin®) for usage in multidose containers. A comprehensive analytical similarity assessment of preserved and unpreserved bevacizumab after dilution and storage was carried out. Methods: Diluted and preserved bevacizumab was analysed at different time points for a 4-week period and compared with unpreserved bevacizumab at the same concentrations at each time point. Native-PAGE, immunoblotting and HP-SEC were used to observe aggregation and degradation. DLS provided information about particle size and dispersity. Bevacizumab quantified by ELISA was conducted to determine its biological activity. Dose response curves and cell migration assays were performed to detect possible toxic effects and determine biological activity and efficacy of the drug using HUVECs. Results: Native-PAGE, immunoblotting and HP-SEC analysis did not show any changes or degradation products in the presence of BAC and after storage compared to unpreserved bevacizumab. The overlapping intensity-based particle size distribution obtained from DLS showed similarity in all tested groups and homogeneity was maintained. ELISA accurately detected bevacizumab at different concentrations. HUVECs incubated with preserved or unpreserved bevacizumab showed a comparable effect on cell migration. No decrease in cell viability was detected. Conclusion: Equivalence tests demonstrated that bevacizumab is stable after dilution, storage and preservation with BAC. Our study shows that preserved bevacizumab applied in mutidose containers can be considered as a cost-effective alternative to the otherwise single-dose treatments. [ABSTRACT FROM AUTHOR]

We describe the contours of one leadership preparation partnership between a university and urban high school district, to demonstrate the affordances that have allowed the partnership to process and weather challenges. Critically, we describe the actual work of collaboration, presenting one extended example of learning-in-action. As such, the article addresses an existing gap in the literature on research practice partnerships, namely how partners can address widespread challenges. Findings suggest that the boundary practice of co-teaching and a "boundary stance" of ontological humility have been critical affordances toward partner and partnership learning. [ABSTRACT FROM AUTHOR]

Summary: Background: Unlike colonic diverticulitis, small bowel diverticulitis is a rare disease, representing only 0.5% of all cases of diverticulitis. Thus, clinicians rarely encounter the disease and no treatment guidelines exist. Meckel's diverticulum is the most common type (about 43%) of small bowel diverticulitis and is often detected incidentally during surgery; duodenal diverticula are the second most common. Symptomatic jejunal diverticulosis, with an incidence of about 0.25% in autopsy studies (of which only about 10% of cases present to hospitals with complications), is even rarer. The literature describes only isolated cases of diverticulitis with involvement throughout most of the small intestine. We report a case involving the entire jejunum/ileum with contained perforation, where diagnostic differentiation from colonic diverticulitis (e.g., with a micro-abscess < 3 cm, typically managed conservatively) was challenging. Unlike colonic diverticulitis, conservative therapy in jejunal diverticulitis carries a mortality rate of up to 40% compared to just 0.5% with surgical intervention. Case report: A 69-year-old female patient presented to the emergency department with acute diffuse abdominal pain, C‑reactive protein (CRP) 11.3 mg/dL, and white blood cell count (WBC) 11.3 G/L. Computed tomography (CT) scan revealed a warren-like abscess conglomerate in the middle abdomen with partial air bubbles in the abscess cavities (differential diagnosis of enterocolic fistulas and contained perforation) and diverticula over almost the entire small intestine. Radiologically, no clear assignment of the pathology or differentiation from a chronic inflammatory disease could be made. After clinical worsening and elevated inflammation parameters following 3 days of antibiotic treatment, open surgical intervention was performed. Intraoperatively, diverticular involvement was observed throughout most of the small intestine, with inflammation extending over about 50 cm of the jejunum and ileum and a 15-cm inflammatory conglomerate tumor with an abscess, necessitating the resection of approximately 60 cm of the small intestine and creation of a side-to-side intestinal anastomosis. Histology showed pseudodiverticula with contained perforation, partial fecal serositis, and multiple abscesses. The patient was discharged on the 10th postoperative day after an uncomplicated stay, receiving i.v. antibiotics for a total of 10 days despite a 3-day therapeutic delay. Follow-up after 14 months was uneventful. Conclusion: Accurate diagnosis can be challenging, and treatment is often delayed due to frequent attempts at conservative management with antibiotics in cases of contained perforated diverticulitis, which is associated with a high rate of complications such as bleeding or perforation (> 50%) in small bowel diverticulitis. However, conservative treatment is typically ineffective in complicated jejunal diverticulitis, whereas it may be more successful in colonic diverticulitis. [ABSTRACT FROM AUTHOR]

Endotypes are characterized by the immunological, inflammatory, metabolic, and remodelling pathways that explain the mechanisms underlying the clinical presentation (phenotype) of a disease. Recessive dystrophic epidermolysis bullosa (RDEB) is a severe blistering disease caused by COL7A1 pathogenic variants. Although underscored by animal studies, the endotypes of human RDEB are poorly understood. To fill this gap, we apply systems immunology approaches using single-cell high-dimensional techniques to capture the signature of peripheral immune cells and the diversity of metabolic profiles in RDEB adults, sampled outside of any opportunistic infection and active cancer. Our study, demonstrates the particular inflammation and immunity characteristics of RDEB adults, with activated / effector T and dysfunctional natural killer cell signatures, concomitant with an overall pro-inflammatory lipid signature. Artificial intelligence prediction models and principal component analysis stress that RDEB is not solely confined to cutaneous issues but has complex systemic endotypes marked by immune dysregulation and hyperinflammation. By characterising the phenotype-endotype association in RDEB adults, our study lays the groundwork for translational interventions that could by lessening inflammation, alleviate the everlasting suffering of RDEB patients, while awaiting curative genetic therapies. Recessive dystrophic epidermolysis bullosa (RDEB) is a severe blistering condition caused by pathogenic variants in the COL7A1 gene and may present with different disease endotypes. In this study, the authors characterise a cohort of RDEB adults to highlight variations in immune cell phenotypes and metabolic profiles, particularly in T cells and NK cells. [ABSTRACT FROM AUTHOR]

As a versatile metal, nickel will experience increased demand in the coming years, with a specific focus on its importance in the battery industry and its role in achieving net-zero emissions. Recognizing the need to ensure sustainable resource management, this study analyses the flow dynamics of nickel's supply and demand by employing a modelling approach. This is done with a focus on understanding how recycling can contribute to meeting the growing nickel demand. By considering the interaction between mining production, product applications, and recycling rates, this study contributes to a better understanding of the long-term prospects for meeting the nickel demand. It can assist policymakers, industry stakeholders, and investors in making informed decisions regarding resource management and developing sustainable practices in the nickel industry. The results revealed that mining would still play an important role in the supply of nickel for at least the next 40 years. Nickel mining and recycling practices are sufficient to meet future nickel demand if sufficient recycling practices are (rapidly) implemented. Modelling results show that nickel recycling will account for 90% of the total demand (primary nickel mining accounts for the remaining 10%) between the years 2062 and 2096. [ABSTRACT FROM AUTHOR]

The article focuses on the ongoing challenges of post-reunification Germany, particularly the experiences of East Germans in the transformation process. Topics include the public perception of East-West differences, the lasting social and economic impacts of reunification, and the role of the "Zukunftszentrum" in addressing these issues through research and cultural reflection.

Purpose: To characterize the burden of uterine fibroids (UF) in individuals experiencing heavy menstrual bleeding (HMB) and moderate-to-severe UF-associated pain in terms of symptoms experienced, impact on work and activities, and pain medication use both on menstrual and non-menstrual days. Patients and Methods: This prospective, real-world, observational study enrolled 350 participants in the USA with a self-reported UF diagnosis, HMB, and moderate-to-severe pain due to UF. Data collection took place from February 9 to July 19, 2021. Over 4 months, participants used an online platform to self-report daily menstrual status, bleeding intensity, UF-associated pain severity, and pain medication use, and to complete weekly work and productivity questionnaires. Results were analyzed descriptively and are reported for the overall population, by pain medication subgroups—defined based on the most potent medication taken—and menstrual versus non-menstrual days/weeks. Results: The analysis population consisted of 307 participants with ≤ 5 consecutive missing days of daily survey responses or ≥ 75% completion rate of the daily surveys. Mean age of participants (standard deviation; SD) was 37.2 (6.3) years. At baseline, 54.1% of participants reported not currently taking medication for treatment of HMB. Over the study period, mean UF-associated pain scores (SD; scale range 0– 10) were higher on menstrual days (3.5 [2.7]) than non-menstrual days (1.0 [1.8]), and this was consistent across medication use subgroups. Pain medications were used more frequently on menstrual days than non-menstrual days (22.9% versus 3.7% days of pain medication use, respectively). Participants reported 31.5% work impairment on menstrual weeks versus a 12.7% work impairment on non-menstrual weeks. Conclusion: In this study, UF-associated pain symptoms coincided with a reduction in individuals' ability to take part in both work and non-work activities and an increase in pain medication use, particularly during menstrual weeks. These results highlight the need for improved diagnosis and pain management strategies in UF. [ABSTRACT FROM AUTHOR]

Dermatological disorders are frequently seen in an emergency care setting. Data suggest that up to 8% of emergency department cases are dermatology-related and may or may not be life-threatening.1 This review focuses on non-IgEmediated life-threatening mucocutaneous hypersensitivity reactions. Dermatology emergencies that involve severe or life-threatening disease include Steven-Johnson syndrome or toxic epidermal necrolysis (SJS/TEN), a drug reaction with eosinophilia and systemic symptoms (DRESS) and acute generalised exanthematous pustulosis (AGEP), which are collectively referred to as severe cutaneous adverse reactions (SCARs). Erythroderma is also discussed in this review article as it represents skin failure that requires prompt management. [ABSTRACT FROM AUTHOR]

Much of what is known about non-resident fathers is based on studies conducted in North America and Europe, and on the accounts of only one family member. This article draws from a study which utilised a family systems perspective and obtained the views of four members of ten Black South African non-resident father families (N = 40) with infrequent father–child contact. The aim was to investigate if and how non-resident fathers' contact with adolescent children was influenced by relationships with various family members. Individual interviews were conducted with each of the family member and thematically analysed. Here, we engage with one of the master themes that shows the circular dynamic in families in which various family members held implicit assumptions about who was responsible for father–child contact and, subsequently, did not view the responsibility to be theirs. Infrequent or lack of non-resident father–child contact should therefore be addressed as a family systems issue. [ABSTRACT FROM AUTHOR]

Summary: Longitudinal studies are subject to nonresponse when individuals fail to provide data for entire waves or particular questions of the survey. We compare approaches to nonresponse bias analysis (NRBA) in longitudinal studies and illustrate them on the Early Childhood Longitudinal Study, Kindergarten Class of 2010–2011 (ECLS‐K:2011). Wave nonresponse with attrition often yields a monotone missingness pattern, and the missingness mechanism can be missing at random (MAR) or missing not at random (MNAR). We discuss weighting, multiple imputation (MI), incomplete data modelling and Bayesian approaches to NRBA for monotone patterns. Weighting adjustments can be effective when the constructed weights are correlated with the survey outcome of interest. MI allows for variables with missing values to be included in the imputation model, yielding potentially less biased and more efficient estimates. We add offsets in the MAR results to provide sensitivity analyses to assess MNAR deviations. We conduct NRBA for descriptive summaries and analytic model estimates in the ECLS‐K:2011 application. The strength of evidence about our NRBA depends on the strength of the relationship between the fully observed variables and the key survey outcomes, so the key to a successful NRBA is to include strong predictors. [ABSTRACT FROM AUTHOR]

During summer 2023 Canada experienced its most intense wildfire season on record. Smoke plumes from these fires advected across the United States (U.S.) Upper Midwest, producing regional scale surface enhancements of PM2.5 and ozone, as recorded by the U.S. surface monitoring network. These events are notable because they occurred early in the fire season (May 15‐June 30), and they produced the highest regional‐scale surface ozone levels ever recorded across the northern tier of the U.S. during early (May–June) or late (July‐August) summer. Specifically, the Upper Midwest 50th ozone percentile was greater than in any other year since 1995, when the ozone monitoring network had sufficient coverage to assess regional‐scale ozone levels; the 90th percentile was the highest since 2002. Satellite and aircraft measurements demonstrate the availability of ozone precursors and ozone production within the smoke plumes. Plain Language Summary: Ozone is a trace gas in the atmosphere that acts as an important greenhouse gas, and high concentrations near Earth's surface are a form of air pollution, detrimental to human health and vegetation productivity. Ozone is formed by sunlight reacting with precursor gases, such as those emitted by fossil fuel combustion. Wildfires are also an important source of ozone precursor gases. During summer 2023 Canada experienced its most intense wildfire season on record. Smoke from these fires impacted the U.S. Upper Midwest during May–June 2023, leading to regional scale surface enhancements of fine particulate matter and ozone. These unusual early season fires produced the highest regional‐scale surface ozone levels ever recorded across the northern U.S. Mid‐latitude wildfires have increased as the planet warms, and their frequency is expected to increase further with continued climate change. This analysis suggests that extreme ozone pollution episodes associated with wildfires could also increase in the future. Key Points: During summer 2023 Canada experienced its most intense wildfire season on recordSmoke from these fires impacted the United States (U.S.) Upper Midwest during May–June, leading to regional scale surface enhancements of PM2.5 and ozoneThese unusual early season fires produced the highest regional‐scale surface ozone levels ever recorded across the northern United States [ABSTRACT FROM AUTHOR]

Aims: Exercise-induced cellular stress and sterile inflammation are of increasing interest. ASC specks are a component of the intracellular NLRP3-inflammasome and can be released into the blood. For example, serum ASC specks are increased after marathon running. We therefore tested whether ASC specks are potentially associated with the individual response to physical training and cardiopulmonary capacity. Methods: We performed a prospective study in 45 healthy athletes. Blood samples were taken before and after cardiopulmonary exercise testing (CPET). ASC speck concentrations were quantitated using flow cytometry. Results: Baseline ASC speck levels correlated with clinical parameters of body composition (height, weight, BMI) and parameters of cardiopulmonary performance (peak VO2, peak oxygen pulse, heart rate after exercise). Athletes with lowest baseline ASC speck concentrations have a significantly lower BMI (22.0 ± 1.8 vs. 24.9 ± 1.6 kg/m2), higher heart rate at rest (72 ± 10 vs. 58 ± 10 beats/min), lower peak VO2 (2692 ± 629 vs. 3404 ± 747 mL/min) and lower peak oxygen pulse (15.6 ± 3.4 vs. 20.7 ± 3.5 mL/heart rate). Overall, ASC speck concentrations showed no significant change after CPET (7.0 ± 4.5 vs. 8.0 ± 5.4 ASC specks/µL, p = 0.3). However, subgroup analysis revealed a significant increase in circulating ASC specks in athletes with the lowest baseline values (2.37 ± 0.84 vs. 8.43 ± 7.52 ASC specks/µL, p < 0.05). Athletes with an increase in ASC speck concentrations in response to CPET had a lower peak oxygen pulse compared to those with a decrease (17.1 ± 4.2 vs. 19.8 ± 4.1, p < 0.05). Conclusion: Low ASC speck baseline values as well as an increase in response to exercise are associated with lower peak oxygen pulse in healthy athletes. [ABSTRACT FROM AUTHOR]

The title compound, catena-poly[[triaquasodium]-di-μ-aqua-[triaquasodium]- μ-(ethane-1,2-diyl)bis[(3-methoxypropyl)phosphinodithiolato]], [Na2(C10H22- O2P2S4)(H2O)8]n, crystallizes in the triclinic space group P1. The dianionic [CH3O(CH2)3P( S)(S—)CH2CH2P( S)(S—)(CH2)3OCH3]2- ligand fragments are joined by a dicationic [Na2(H2O)8]2+ cluster that includes the oxygen of the methoxypropyl unit of the ligand to form infinite chains. [ABSTRACT FROM AUTHOR]

Mental health is a component of care that should be addressed for patients with burns while they are hospitalized. Unfortunately, dedicated burn psychotherapists are rare in burn centers in the United States (US), and it can take months for patients to be seen by a mental health professional after referral. Our burn center has a dedicated licensed clinical social worker who sees patients within 2 business days of referral. She uses cognitive behavioral therapy (CBT), which is designed to alleviate symptoms of anxiety, depression, and acute stress by modifying the individual's maladaptive thoughts. To evaluate the timely use of CBT as a treatment for depression in patients with burns, we measured depressive symptoms before and after psychotherapy. Burn clinic nurses administered the Patient Health Questionnaire (PHQ-9) depression screener as part of standard care. We computed difference scores to determine the change in PHQ-9 scores at both group and individual levels. At a group level, psychotherapy significantly improved symptoms of depression, indicated by a decreased mean PHQ-9 score. On an individual level, half of the patients (50.7%) experienced a meaningful improvement in their symptoms, indicated by a change in their PHQ-9 depression category, while 35.6% showed no change. Although it was not an effective solution for all patients in this study, timely use of CBT could be an important component of burn care for many and should be considered as part of standard care in burn centers across the US. [ABSTRACT FROM AUTHOR]

Study Design: Systematic review. Objectives: Intraoperative neuromonitoring (IOMN) has become a standard practice in the detection and prevention of nerve damage and postoperative deficit. While multicenter studies have addressed this inquiry, there have been no systematic reviews to date. This systematic review identifies the leading causes of IONM alerts during adult spinal deformity (ASD) surgeries. Methods: Following PRISMA guidelines, a literature search was performed in PubMed and Embase. IONM alert causes were grouped by equivalent terms used across different studies and binned into larger categories, including surgical maneuver, Changes in blood pressure/temperature, Oxygenation, Anesthesia, Patient position, and Unknown. Results: Inclusion criteria were studies on adult patients receiving ASD correction surgery using IONM with documented alert causes. 1544 references were included in abstract review, 128 in full text review, and 16 studies qualified for data extraction. From those studies, there was a total of 3945 adult patients with 299 IONM alerts. Surgical maneuver led the alert causes (258 alerts/86.3%), with signal loss most commonly occurring at correction or osteotomy (101/33.8% and 95/31.8% respectively). Pedicle screw placement caused 35 alerts (11.7%). Changes in temperature and blood pressure were the third largest category (34/11.4%). Conclusions: The most frequent causes of IONM alerts in ASD surgery were surgical maneuvers such as correction, osteotomy, and pedicle screw placement. This information provides spine surgeons with a quantitative perspective on the causes of IONM changes and show that most occur at predictable times during ASD surgery. [ABSTRACT FROM AUTHOR]

The article presents a case study of a 17-year-old male who developed perforated appendicitis during the re-nourishment phase of treatment for an eating disorder. Topics discussed include the patient's significant weight loss and dietary practices, the medical team's management of his bradycardia and nutritional rehabilitation, and the complications that arose during hospitalization, ultimately leading to emergency surgery.

The Old Melbourne Gaol maintains a crucial place in social histories of the city. The Gaol ceased operation in 1924 and, as a heritage site, locates its history firmly in the nineteenth and early-twentieth centuries. While the exhibition invites a moral consideration of modes of punishment implemented in the gaol, its critique suggests resolution: these are not practices that we engage in now. By presenting these kinds of closed or finished narratives, the site turns visitors firmly towards the past rather than creating a site which invites reflection on the harms that carceral institutions continue to inflict in the present day. This, we argue, has a particular significance for Care-leavers, who are a significant demographic to have passed through the Gaol's walls. While recognising that a site like the Old Melbourne Gaol has innumerable memory communities invested in it, and that no presentation of the site might fully satisfy them all, this article argues for present-looking public histories that place the gaol's history in conversation with weighty social justice issues of today, including acknowledging the gaol's role as part of the foundations of the 'care'-to-prison pipeline observed by many activists as a pressing social issue in the twenty-first century. [ABSTRACT FROM AUTHOR]

Angiogenesis plays a crucial role in various physiological and pathological conditions. However, research in equine angiogenesis is relative limited, necessitating the development of suitable in-vitro models. To effectively analyze angiogenesis invitro, it is essential to target the specific cells responsible for this process, namely endothelial cells. Human umbilical vein endothelial cells (HUVECs) are one of the most used in vitro models for studying angiogenesis in humans. Serving as an equivalent to HUVECs, we present a comprehensive isolation protocol for equine umbilical vein endothelial cells (EqUVECs) with relatively minimal requirements, thereby enhancing accessibility for researchers. Umbilical cords obtained from five foals were used to isolate endothelial cells, followed by morphological and immunohistochemical identification. Performance of the cells in various assays commonly used in angiogenesis research was studied. Additionally, EqUVEC expression of vascular endothelial growth factor (VEGF) was assessed using ELISA. EqUVECs exhibited endothelial characteristics, forming a homogeneous monolayer with distinctive morphology. Immunohistochemical staining confirmed positive expression of key endothelial markers including von Willebrand factor (vWF), CD31, and vascular endothelial growth factor receptor-2 (VEGFR-2). Furthermore, performance assessments in in-vitro assays demonstrated the viability, proliferation, migration, tube formation and VEGF-expression capabilities of EqUVECs. The findings suggest that EqUVECs are a promising in-vitro model for studying equine angiogenesis, offering a foundation for further investigations into equine-specific vascular processes and therapeutic interventions. [ABSTRACT FROM AUTHOR]

Aims: Exercise-induced cellular stress and sterile inflammation are of increasing interest. ASC specks are a component of the intracellular NLRP3-inflammasome and can be released into the blood. For example, serum ASC specks are increased after marathon running.We therefore tested whether ASC specks are potentially associated with the individual response to physical training and cardiopulmonary capacity. Methods: We performed a prospective study in 45 healthy athletes. Blood samples were taken before and after cardiopulmonary exercise testing (CPET). ASC speck concentrations were quantitated using flow cytometry. Results: Baseline ASC speck levels correlated with clinical parameters of body composition (height, weight, BMI) and parameters of cardiopulmonary performance (peak VO2, peak oxygen pulse, heart rate after exercise). Athletes with lowest baseline ASC speck concentrations have a significantly lower BMI (22.0 ± 1.8 vs. 24.9 ± 1.6 kg/m2), higher heart rate at rest (72 ± 10 vs. 58 ± 10 beats/ min), lower peak VO2 (2692 ± 629 vs. 3404 ± 747 mL/min) and lower peak oxygen pulse (15.6 ± 3.4 vs. 20.7 ± 3.5 mL/heart rate). Overall, ASC speck concentrations showed no significant change after CPET (7.0 ± 4.5 vs. 8.0 ± 5.4 ASC specks/µL, p = 0.3). However, subgroup analysis revealed a significant increase in circulating ASC specks in athletes with the lowest baseline values (2.37 ± 0.84 vs. 8.43 ± 7.52 ASC specks/µL, p < 0.05). Athletes with an increase in ASC speck concentrations in response to CPET had a lower peak oxygen pulse compared to those with a decrease (17.1 ± 4.2 vs. 19.8 ± 4.1, p < 0.05). Conclusion: Low ASC speck baseline values as well as an increase in response to exercise are associated with lower peak oxygen pulse in healthy athletes. [ABSTRACT FROM AUTHOR]

Ebola virus (EBOV) infection results in Ebola virus disease (EVD), an often severe disease with a nonspecific presentation. Since its recognition, periodic outbreaks of EVD continue to occur in sub-Saharan Africa. The 2013–2016 West African EVD outbreak was the largest recorded, resulting in a substantial cohort of EVD survivors with persistent health complaints and variable immune responses. In this study, we characterize humoral immune responses in EVD survivors and their contacts in Eastern Sierra Leone. We found high levels of EBOV IgG in EVD survivors and lower yet substantial antibody levels in household contacts, suggesting subclinical transmission. Neutralizing antibody function was prevalent but variable in EVD survivors, raising questions about the durability of immune responses from natural infection with EBOV. Additionally, we found that certain discrete symptoms—ophthalmologic and auditory—are associated with EBOV IgG seropositivity, while an array of symptoms are associated with the presence of neutralizing antibody. [ABSTRACT FROM AUTHOR]

This paper examines the organizational identity orientations (IDOs) of members in the higher education context. We conducted a survey of students, faculty, and staff at a large public university to explore their perceptions of the university's identity at a time of organizational change under new leadership and investigated how they compared on the individualistic, relational, and collectivistic IDOs. Analysis revealed that faculty and students perceived the university more positively and shared similar rankings of its values in comparison to staff. Additionally, the level of students' collectivistic IDO was higher than that of faculty, and students' relational IDO was higher than that of both faculty and staff. Lastly, the frequency of interaction among members of the same stakeholder group was negatively related to the relational IDO. The theoretical and practical implications of this study contribute to the literature on organizational identification and identity orientation among university stakeholders. [ABSTRACT FROM AUTHOR]

Despite progress in reducing youth tobacco use, the epidemic persists as a pressing public health challenge, exacerbated by the rise of vaping and flavored products. This article synthesizes the latest evidence on effective youth tobacco control interventions and presents a detailed examination of the comprehensive initiatives implemented in Mississippi over the past six years. Data from the Mississippi State Department of Health's Office of Tobacco Control program evaluations from 2018-2023 are presented, covering school curricula, community events, peer education, advocacy, media campaigns, and other activities delivered by tobacco-free coalitions, physician groups, and partner organizations statewide. Over this period, these multi-level initiatives reached tens of thousands of youths annually through evidence-based programs like CATCH My Breath® and Tar Wars®, as well as awareness presentations, leadership training, policy advocacy, digital content, and more. Evolving strategies included increased virtual programming and social media engagement during the COVID-19 pandemic. While evaluation limitations exist around long-term impacts, Mississippi's multifaceted approach reflects expertrecommended practices of combining school/community interventions with youth engagement, professional education, media outreach, and policy change. As tobacco landscapes shift, such comprehensive, adaptable strategies remain crucial to protecting the next generation from tobacco, e-cigarette, and vaping addiction's harms. Continued investment in innovative, evidence-based youth tobacco control programming is vital. [ABSTRACT FROM AUTHOR]

Relatively little work has focused on why we are motivated to learn words. In adults, recent experiments have shown that intrinsic reward signals accompany successful word learning from context. In addition, the experience of reward facilitated long‐term memory for words. In adolescence, developmental changes are seen in reward and motivation systems as well as in reading and language systems. Here, in the face of this developmental change, we ask whether adolescents experience reward from word learning, and how the reward and memory benefit seen in adults is modulated by age. We used a naturalistic reading paradigm, which involved extracting novel word meanings from sentence context without the need for explicit feedback. By exploring ratings of enjoyment during the learning phase, as well as recognition memory for words a day later, we assessed whether adolescents show the same reward and learning patterns as adults. We tested 345 children between the ages of 10–18 (N > 84 in each 2‐year age‐band) using this paradigm. We found evidence for our first prediction: children aged 10–18 report greater enjoyment for successful word learning. However, we did not find evidence for age‐related change in this developmental period, or memory benefits. This work gives us greater insight into the process of language acquisition and sets the stage for further investigations of intrinsic reward in typical and atypical development. Research Highlights: We constantly learn words from context, even in the absence of explicit rewards or feedback.In adults, intrinsic reward experienced during word learning is linked to a dopaminergic circuit in the brain, which also fuels enhancements in memory for words.We find adolescents also report enhanced reward or enjoyment when they successfully learn words from sentence context.The relationship between reward and learning is maintained between the ages of 10 and 18.Unlike in adults, we did not observe ensuing memory benefits. [ABSTRACT FROM AUTHOR]

Objectives: To investigate the effect of emotion regulation skills‐focused (ERSF) interventions to reduce pain intensity and improve psychological outcomes for people with chronic pain and to narratively report on safety and intervention compliance. Methods: Six databases and four registries were searched for randomized controlled trials (RCTs) up to 29 April 2022. Risk of bias was evaluated using the Cochrane RoB 2.0 tool, and certainty of evidence was assessed according to the Grading, Assessment, Development and Evaluation (GRADE). Meta‐analyses for eight studies (902 participants) assessed pain intensity (primary outcome), emotion regulation, affect, symptoms of depression and anxiety, and pain interference (secondary outcomes), at two time points when available, post‐intervention (closest to intervention end) and follow‐up (the first measurement after the post‐intervention assessment). Results: Compared to TAU, pain intensity improved post‐intervention (weighted mean difference [WMD] = −10.86; 95% confidence interval [CI] [−17.55, −2.56]) and at follow‐up (WMD = −11.38; 95% CI [−13.55, −9.21]). Emotion regulation improved post‐intervention (standard mean difference [SMD] = 0.57; 95% CI [0.14, 1.01]), and depressive symptoms improved at follow‐up (SMD = −0.45; 95% CI [−0.66, −0.24]). Compared to active comparators, anxiety symptoms improved favouring the comparator post‐intervention (SMD = 0.10; 95% CI [0.03, 0.18]), and compared to CBT, pain interference improved post‐intervention (SMD = −0.37; 95% CI [−0.69, −0.04]). Certainty of evidence ranged from very low to moderate. Significance: The findings provide evidence that ERSF interventions reduce pain intensity for people with chronic pain compared to usual treatment. These interventions are at least as beneficial to reduce pain intensity as the current gold standard psychological intervention, CBT. However, the limited number of studies and certainty of evidence mean further high‐quality RCTs are warranted. Additionally, further research is needed to identify whether ERSF interventions may be more beneficial for specific chronic pain conditions. [ABSTRACT FROM AUTHOR]

Background: The World Health Organization (WHO) recommends that women with HIV breastfeed for a minimum of one year. In contrast, across high-income countries, HIV and infant-feeding guidelines recommend exclusive formula feeding if parents want to avoid all risk of postpartum transmission. However, recently these guidelines (including in the United Kingdom (UK)) increasingly state that individuals with HIV should be supported to breast/chest feed if they meet certain criteria; such as an undetectable maternal HIV viral load and consent to additional clinical monitoring. Between 600 and 800 pregnancies are reported annually in women with HIV in the UK, with low rates of vertical transmission (0.22%). Informed infant-feeding decision-making requires clinical support. Currently, little research addresses how individuals with HIV in high-income countries navigate infant-feeding decisions with their clinical teams and familial and social networks, and the resources needed to reach an informed decision. Methods: Semi-structured remote interviews were conducted between April 2021 – January 2022 with UK-based individuals with a confirmed HIV diagnosis who were pregnant or one-year postpartum. Using purposive sampling, pregnant and postpartum participants were recruited through NHS HIV clinics, community-based organisations and snowballing. Data were analysed thematically and organised using NVivo 12. Results: Of the 36 cisgender women interviewed, 28 were postpartum. The majority were of Black African descent (n = 22) and born outside the UK. The majority of postpartum women had chosen to formula feed. Women's decision-making regarding infant-feeding was determined by (1) information and support; (2) practicalities of implementing medical guidance; (3) social implications of infant-feeding decisions. Conclusion: The evolution of UK HIV and infant-feeding guidelines are not reflected in the experiences of women living with HIV. Clinicians' emphasis on reducing the risk of vertical transmission, without adequately considering personal, social and financial concerns, prevents women from making fully informed infant-feeding decisions. For some, seeking advice beyond their immediate clinical team was key to feeling empowered in their decision. The significant informational and support need among women with HIV around their infant-feeding options must be addressed. Furthermore, training for and communication by healthcare professionals supporting women with HIV is essential if women are to make fully informed decisions. [ABSTRACT FROM AUTHOR]

The Solar eruptioN Integral Field Spectrograph (SNIFS) is a solar-gazing spectrograph scheduled to fly in the summer of 2025 on a NASA sounding rocket. Its goal is to view the solar chromosphere and transition region at a high cadence (1 s) both spatially ( 0.5 ″ ) and spectrally (33 mÅ) viewing wavelengths around Lyman alpha (1216 Å), Si iii (1206 Å), and O v (1218 Å) to observe spicules, nanoflares, and possibly a solar flare. This time cadence will provide yet-unobserved detail about fast-changing features of the Sun. The instrument is comprised of a Gregorian-style reflecting telescope combined with a spectrograph via a specialized mirrorlet array that focuses the light from each spatial location in the image so that it may be spectrally dispersed without overlap from neighboring locations. This paper discusses the driving science, detailed instrument and subsystem design, and preintegration testing of the SNIFS instrument. [ABSTRACT FROM AUTHOR]

Background: Until now, limited clinical significance had been reported for disseminated tumor cells (DTCs) in gynecologic malignancies. DTCs were previously reported not to be associated with established risk factors, L1CAM immunoreactivity, and outcome in endometrial carcinoma (EC). This study's primary objective was to investigate potential correlations of DTCs in the bone marrow (BM) of EC patients with disease-related survival, and a secondary objective was to evaluate associations between molecular classification of EC and DTCs. Methods: Patients treated for primary EC at Tuebingen University women's hospital between 2003 and 2016 were identified. A total of 402 patients with a complete set of BM cytology, molecular, and clinical data were evaluable. Results: DTC occurrence was distributed equally among all four molecular groups (p = 0.651). DTC positivity was associated with a less favorable disease-free survival (HR: 1.86, 95% CI: 1.03–3.36, p = 0.036) and progression-free survival (HR: 1.86, 95% CI: 1.01–3.44, p = 0.045). Presence of DTCs was associated with a higher frequency of distant disease recurrence (p = 0.017). Conclusions: In line with our previous findings, tumor cell dissemination is not associated with molecular features in our large cohort of primary EC patients. Since DTCs seem to be associated with survival and location of disease recurrence, further studies are needed to decisively define their role in EC survival. [ABSTRACT FROM AUTHOR]

Background: Emotional intelligence (EI) of physicians significantly impacts their personal well‐being and professional success with broad implications in health care. A focused training on EI is often lacking in medical curricula. We sought to understand the impact of improvisation training on clinicians' EI. Approach: Four online medical improv workshops were offered to a diverse group of physicians with varied levels of practice experience including medicine‐paediatric residents, paediatric educators, practising paediatricians and internal/family medicine clinicians. The improv training was thoughtfully curated and remained consistent for all four cohorts, lasting 2 h. Self‐reported EI scales (pre and post) were captured using an online survey tool. The overall EI score and the scores of three EI components were compared before and after training. Evaluation: Out of 64 participants, 41 participants (64%) completed both the pre‐ and post‐surveys and were included in the final analysis. Participant's pre‐training score (mean:123.9, range: 121.1–126.7) was compared to their post‐training score (mean:128.9, range: 126.3–131.3). The t tests comparing EI scores showed that compared to pre‐intervention, participants on average scored 4.9 points higher (95% CI: 3.1–6.7; p < 0.01) on the overall scale, 2.2 points higher (95% CI: 1.2–3.2; p < 0.01) on the appraisal score, 1.4 points higher (95% CI: 0.8–2.0; p < 0.01) on the regulation score and 1.2 points higher (95% CI: 0.4–2.1; p = 0.01) on the utilisation score. Implications: Improv training is an innovative method to fill the crucial gap in EI curricula. There was a statistically significant improvement in average score for clinicians' EI after a pilot improv training programme. [ABSTRACT FROM AUTHOR]

Background: The human oral and nasal cavities can act as reservoirs for opportunistic pathogens capable of causing acute infection. These microbes asymptomatically colonize the human oral and nasal cavities which facilitates transmission within human populations via the environment, and they routinely possess clinically significant antibiotic resistance genes. Among these opportunistic pathogens, the Klebsiella genus stands out as a notable example, with its members frequently linked to nosocomial infections and multidrug resistance. As with many colonizing opportunistic pathogens, the essential transmission factors influencing the spread of Klebsiella species among both healthy and diseased individuals remain unclear. Results: Here, we explored a possible explanation by investigating the ability of oral and nasal Klebsiella species to outcompete their native microbial community members under in vitro starvation conditions, which could be analogous to external hospital environments or the microenvironment of mechanical ventilators. When K. pneumoniae and K. aerogenes were present within a healthy human oral or nasal sample, the bacterial community composition shifted dramatically under starvation conditions and typically became enriched in Klebsiella species. Furthermore, introducing K. pneumoniae exogenously into a native microbial community lacking K. pneumoniae, even at low inoculum, led to repeated enrichment under starvation. Precise monitoring of K. pneumoniae within these communities undergoing starvation indicated rapid initial growth and prolonged viability compared to other members of the microbiome. K. pneumoniae strains isolated from healthy individuals' oral and nasal cavities also exhibited resistance to multiple classes of antibiotics and were genetically similar to clinical and gut isolates. In addition, we found that in the absence of Klebsiella species, other understudied opportunistic pathogens, such as Peptostreptococcus, increased in relative abundance under starvation conditions. Conclusions: Our findings establish an environmental and microbiome community circumstance that allows for the enrichment of Klebsiella species and other opportunistic pathogens. Klebsiella's enrichment may hinge on its ability to quickly outgrow other members of the microbiome. The ability to outcompete other commensal bacteria and to persist under harsh environmental conditions could be an important factor that contributes to enhanced transmission in both commensal and pathogenic contexts. 2NMbQE6jVjinwr48dC_rNo Video Abstract [ABSTRACT FROM AUTHOR]

As an evolutionarily ancient sense, olfaction is key to learning where to find food, shelter, mates, and important landmarks in an animal's environment. Brain circuitry linking odor and navigation appears to be a well conserved multiregion system among mammals; the anterior olfactory nucleus, piriform cortex, entorhinal cortex, and hippocampus each represent different aspects of olfactory and spatial information. We review recent advances in our understanding of the neural circuits underlying odor-place associations, highlighting key choices of behavioral task design and neural circuit manipulations for investigating learning and memory. [ABSTRACT FROM AUTHOR]

Objectives: this scoping review aimed to explore the diversity of existing nature-based mindfulness (NBM) interventions. The specific objectives of this review were to (1) describe the practices and methods that are used in NBM interventions, and to (2) determine the environmental conditions that are typically associated with NBM interventions. Method: Thirty peer-reviewed scientific studies were identified via a systematic PRISMA search protocol and then thematically analysed and categorically organised. Results: In relation to the first research objective, a typological scheme for classifying NBM interventions was proposed in which four main categorizations of NBM interventions were identified, including (1) conventional practices combined with nature, (2) activity-based practices using nature, (3) NBM therapy practices, and (4) emerging practices. These themes demonstrate the diversity of existing NBM interventions and provide a more integrated understanding of the applicability of these interventions across different clinical and non-clinical contexts. In relation to the second research objective, existing NBM interventions were found to be conducted in (1) naturally occurring, (2) curated natural, and (3) simulated natural environments. Within these categories, a diverse range of restorative environments were identified as suitable contexts for NBM interventions, with forest-based interventions being the most commonly used environment. Conclusions: Overall, this study contributes to a more integrated understanding of the practices, methods, and environmental conditions typical of existing NBM interventions, proposes a classification scheme for NBM interventions, and identifies a number of new developments within the field as well as promising avenues for future research and practice. Preregistration: This study has not been preregistered. [ABSTRACT FROM AUTHOR]

We present an extension to the Brain Imaging Data Structure (BIDS) for motion data. Motion data is frequently recorded alongside human brain imaging and electrophysiological data. The goal of Motion-BIDS is to make motion data interoperable across different laboratories and with other data modalities in human brain and behavioral research. To this end, Motion-BIDS standardizes the data format and metadata structure. It describes how to document experimental details, considering the diversity of hardware and software systems for motion data. This promotes findable, accessible, interoperable, and reusable data sharing and Open Science in human motion research. [ABSTRACT FROM AUTHOR]

Assessing the impact of SARS-CoV-2 on organelle dynamics allows a better understanding of the mechanisms of viral replication. We combine label-free holotomographic microscopy with Artificial Intelligence to visualize and quantify the subcellular changes triggered by SARS-CoV-2 infection. We study the dynamics of shape, position and dry mass of nucleoli, nuclei, lipid droplets and mitochondria within hundreds of single cells from early infection to syncytia formation and death. SARS-CoV-2 infection enlarges nucleoli, perturbs lipid droplets, changes mitochondrial shape and dry mass, and separates lipid droplets from mitochondria. We then used Bayesian network modeling on organelle dry mass states to define organelle cross-regulation networks and report modifications of organelle cross-regulation that are triggered by infection and syncytia formation. Our work highlights the subcellular remodeling induced by SARS-CoV-2 infection and provides an Artificial Intelligence-enhanced, label-free methodology to study in real-time the dynamics of cell populations and their content. Studying the impact of SARS-CoV-2 on organelle dynamics may shed more light on the mechanisms of viral replication. Here, the authors combine label-free holotomographic microscopy with AI to study subcellular changes and organelle dynamics upon SARS-CoV-2 infection. [ABSTRACT FROM AUTHOR]

Background: Laboratory results are frequently abnormal in pregnant mothers. Abnormalities usually relate to pregnancy or associated complications. Hematological abnormalities and age in pregnancy may increase the likelihood for transfusion and mortality. Study Design and Methods: Hematological profiles and transfusion history of pregnant mothers presenting to a tertiary hospital, were evaluated over 2 years. Age, anemia, leukocytosis and thrombocytopenia were assessed for transfusion likelihood. Iron deficiency and coagulation were assessed in transfused patients. Anemia, leukocytosis, thrombocytopenia, human immunodeficiency virus (HIV) and transfusion were assessed for mortality likelihood. Results: There were 12,889 pregnant mothers included. Mothers <19‐years‐old had the highest prevalence of anemia (31.5%) and proportion of transfusions (19%). The transfusion likelihood was increased in mothers with anemia (odds ratios [OR] = 6.41; confidence intervals at 95% [95% CI] 5.46–7.71), leukocytosis (OR = 2.35; 95% CI 2.00–2.76) or thrombocytopenia (OR = 2.71; 95% CI 2.21–3.33). Mothers with prolonged prothrombin times received twice as many blood products as their normal counterparts (p =.03) and those with iron deficiency anemia five times more blood products (p <.001). Increased likelihood for mortality was seen in patients with anemia (OR = 4.15, 95% CI 2.03–8.49), leukocytosis (OR = 2.68; 95% CI 1.19–6.04) and those receiving blood transfusion (OR = 3.6, 95% CI 1.75–7.47). Discussion: Adolescence, anemia, leukocytosis and thrombocytopenia expose mothers to a high risk for transfusion and/or mortality. These risk factors should promptly trigger management and referral of patients. Presenting hematological profiles are strong predictors of maternal outcome and transfusion risk. [ABSTRACT FROM AUTHOR]

Chordomas are very rare malignant neoplasms of the bone occurring almost exclusively along the spine. As the tumours are thought to arise from notochordal remnants, the vast majority of chordomas express the TBXT gene, resulting in detectable nuclear amounts of its gene product brachyury. This T-Box transcription factor is commonly recognised as being essential in chordoma cells, and limiting TBXT expression is thought to be the key factor in controlling this tumour. Although the tumour is rare, distinct molecular differences and vulnerabilities have been described with regard to its location and the progression status of the disease, rendering it mandatory for novel cell lines to reflect all relevant chordoma subtypes. Here, we describe a novel chordoma cell line arising from the pleural effusion of a disseminated, poorly differentiated chordoma. This cell line, U-CH22, represents a highly aggressive terminal chordoma and, therefore, fills a relevant gap within the panel of available cell culture models for this orphan disease. CDK7 and CDK9 inhibition was lately identified as being effective in reducing viability in four chordoma cell lines, most likely due to a reduction in brachyury levels. In this study, we determined the capability of the CDK7 inhibitor THZ1 and the CDK1/2/5/9 inhibitor dinaciclib to reduce TBXT expression at mRNA and protein levels in a broad range of nine cell lines that are models of primary, recurrent, and metastasised chordoma of the clivus and the sacrum. [ABSTRACT FROM AUTHOR]

Background: In 2020, during the COVID-19 pandemic, Médecins Sans Frontières (MSF) initiated three cycles of dihydroartemisin-piperaquine (DHA-PQ) mass drug administration (MDA) for children aged three months to 15 years within Bossangoa sub-prefecture, Central African Republic. Coverage, clinical impact, and community members perspectives were evaluated to inform the use of MDAs in humanitarian emergencies. Methods: A household survey was undertaken after the MDA focusing on participation, recent illness among eligible children, and household satisfaction. Using routine surveillance data, the reduction during the MDA period compared to the same period of preceding two years in consultations, malaria diagnoses, malaria rapid diagnostic test (RDT) positivity in three MSF community healthcare facilities (HFs), and the reduction in severe malaria admissions at the regional hospital were estimated. Twenty-seven focus groups discussions (FGDs) with community members were conducted. Results: Overall coverage based on the MDA card or verbal report was 94.3% (95% confidence interval (CI): 86.3–97.8%). Among participants of the household survey, 2.6% (95% CI 1.6–40.3%) of round 3 MDA participants experienced illness in the preceding four weeks compared to 30.6% (95% CI 22.1–40.8%) of MDA non-participants. One community HF experienced a 54.5% (95% CI 50.8–57.9) reduction in consultations, a 73.7% (95% CI 70.5–76.5) reduction in malaria diagnoses, and 42.9% (95% CI 36.0–49.0) reduction in the proportion of positive RDTs among children under five. A second community HF experienced an increase in consultations (+ 15.1% (− 23.3 to 7.5)) and stable malaria diagnoses (4.2% (3.9–11.6)). A third community HF experienced an increase in consultations (+ 41.1% (95% CI 51.2–31.8) and malaria diagnoses (+ 37.3% (95% CI 47.4–27.9)). There were a 25.2% (95% CI 2.0–42.8) reduction in hospital admissions with severe malaria among children under five from the MDA area. FGDs revealed community members perceived less illness among children because of the MDA, as well as fewer hospitalizations. Other indirect benefits such as reduced household expenditure on healthcare were also described. Conclusion: The MDA achieved high coverage and community acceptance. While some positive health impact was observed, it was resource intensive, particularly in this rural context. The priority for malaria control in humanitarian contexts should remain diagnosis and treatment. MDA may be additional tool where the context supports its implementation. [ABSTRACT FROM AUTHOR]