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Cellular deconvolution via bulk RNA sequencing (RNA-seq) presents a cost-effective and efficient alternative to experimental methods such as flow cytometry and single-cell RNA-seq (scRNA-seq) for analyzing the complex cellular composition of tumor microenvironments. Despite challenges due to heterogeneity within and among tumors, our innovative deep learning-based approach, DeSide, shows exceptional accuracy in estimating the proportions of 16 distinct cell types and subtypes within solid tumors. DeSide integrates biological pathways and assesses noncancerous cell types first, effectively sidestepping the issue of highly variable gene expression profiles (GEPs) associated with cancer cells. By leveraging scRNA-seq data from six cancer types and 185 cancer cell lines across 22 cancer types as references, our method introduces distinctive sampling and filtering techniques to generate a high-quality training set that closely replicates real tumor GEPs, based on The Cancer Genome Atlas (TCGA) bulk RNA-seq data. With this model and high-quality training set, DeSide outperforms existing methods in estimating tumor purity and the proportions of noncancerous cells within solid tumors. Our model precisely predicts cellular compositions across 19 cancer types from TCGA and proves its effectiveness with multiple additional external datasets. Crucially, DeSide enables the identification and analysis of combinatorial cell type pairs, facilitating the stratification of cancer patients into prognostically significant groups. This approach not only provides deeper insights into the dynamics of tumor biology but also highlights potential therapeutic targets by underscoring the importance of specific cell type or subtype interactions. [ABSTRACT FROM AUTHOR]

Gait recognition is a biometric approach used to identify people based on their walking patterns at long distances and low resolutions. Most advanced gait recognition methods based on silhouettes employ the focal convolution module. However, experiments have demonstrated that the horizontal segmentation method used in this module causes information loss at the feature map demarcation line. In this paper, we propose an adaptive feature fusion block (AFFB) for feature extraction that utilizes comprehensive global features to compensate for the lost local features, significantly reducing feature loss caused by local convolution. Additionally, we introduce a feature expansion module (FEM) to enrich the temporal information of gait features and adaptively balance the body detail information extracted by the model with the overall body information. We evaluated our model on CASIA-B and OU-MVLP datasets and compared it to other gait models using RANK-1 accuracy. The experimental results show that our model can represent gait features better than other models and achieved high accuracy in gait recognition across perspectives and various walking conditions.The source code will be available on https://github.com/Lentia/AdaptiveGait. [ABSTRACT FROM AUTHOR]

Toxocara canis is an intestinal roundworm that can cause serious zoonotic parasitic diseases. Drontal Plus® Tasty (Dog) is a kind of commercial drug used to treat T. canis infection. Febantel, Praziquantel, and Pyrantel pamoate (PP) are its main component. However, there are few studies investigating the impact of Drontal Plus® Tasty (Dog) and its primary ingredients on the intestinal microbiota of dogs. In this study, we first collected the intestinal content samples of the dogs which administrated with anthelmintics or saline by sterile catheters, then used 16S rRNA high-throughput sequencing technology combined with a variety of bioinformatic analysis methods to analyze the effect of anthelmintics on intestinal microbiota. First, the results of the α and β diversity analysis showed that the abundance and diversity of intestinal microbiota decreased with T. canis infection, and increased after anthelmintic treatment. Then, we found the dominant species (the value of relative abundance > 0.05) was both 28 on phylum and genus levels, besides the most dominant species was Bacillota on phylum level and Segatella and Clostridium_sensu_stricto were most dominant on genus level. Futher analyzing the differences in microbiotal composition on phylum level, we found that Drontal Plus® Tasty treatment could significantly increase the proportion of Bacillota, while Febantel, Praziquantel, or PP could induce the significantly changes of Bacillota and Bacteroidota. In addition, by analyzing the differences in microbiotal composition on genus level, we found that anthelmintic could significantly decreased the relative abundance of Clostridium_sensu_stricto and significantly increased the abundance of Segatella. However, Drontal Plus® Tasty had no regulatory effect on the abundance of Segatella. In short, these finding showed that various anthelmintics all have significant effects for changing the abundance and diversity of host intestinal microbiota. [ABSTRACT FROM AUTHOR]

Iron-based anode materials usually exhibit an unsatisfactory rate performance and poor cycle stability during Li+ storage. Herein, ultrathin hexagonal Fe3O4 nanosheets are embedded in nitrogen-doped carbon nanofibers (hexa-Fe3O4@N-CNF), boosting the Li+ diffusion kinetics and maintaining cycling stability. The ultrathin hexa-Fe3O4 nanosheets possess a short Li+ diffusion pathway, while N-CNF displays excellent conductivity, both of which contribute to the rapid insertion/extraction of Li+. The iron-induced thermal-catalyzed N-CNF manifests enhanced mechanical stability due to its increased graphitization degree, which effectively alleviates the mechanical stress resulting from the volume change of the hexa-Fe3O4 during Li+ storage, thereby enhancing the cyclic stability of hexa-Fe3O4@N-CNF. The compressed anode of hexa-Fe3O4@N-CNF presents a high mass loading (3.3 mg cm–2) and is coupled with a flexible cathode (activated carbon casted onto N-CNF) to assemble the quasi-solid-state lithium-ion hybrid capacitor (LIHC), enabling the attainment of high volumetric power/energy density (66.5 W h L–1 at 117.9 W L–1 and 38.5 W h L–1 at 11785.7 W L–1). When the LIHC is bent at 180°, its Li+ storage performance illustrates a negligible degradation. Additionally, LIHC features a low self-discharge rate (∼14.2 mV h–1). Overall, the long-term cycle stability, high power/energy density, low self-discharge rate, as well as mechanical robustness render this LIHC a suitable candidate for flexible energy storage applications. [ABSTRACT FROM AUTHOR]

The piezotronics effect utilizes a piezopotential to modulate and control current in piezo-semiconductors. Ferroelectric materials, as a type of piezoelectric materials, possess piezoelectric coefficients that are significantly larger than those found in conventional piezoelectric materials. Here, we propose a strain modulated ferroelectric field-effect transistor (St-FeFET) utilizing external strain instead of gate voltage to achieve ferroelectric modulation, which eliminates the need for gate voltage. By applying a very small strain (0.01%), the St-FeFET can achieve a maximum on-off current ratio of 1250% and realizes a gauge factor (GF) of 1.19 × 106, which is much higher than that of conventional strain sensors. This work proposes a new method for realizing highly sensitive strain sensors and presents innovative approaches to the operation methods of ferroelectric field-effect transistors as well as potential applications for coupling of strain sensors and various devices across different fields. [ABSTRACT FROM AUTHOR]

Summary: As one of major construction facilities, the safety concern about elevator outer‐attached to slender high‐rise buildings during construction needs to be seriously addressed. To specifically identify the risk of such slender building–elevator systems in extreme tornado wind field, a safety assessment strategy using the critical velocity envelope is systematically developed. To overcome the difficulties in modeling such building–elevator system, a modified generalized flexural‐shear model developed previously was employed to reach an efficient estimate on the response of a case high‐rise building under simulated tornado wind excitations. To account for the effect of the flexibility of high‐rise building on the response of its attached construction elevator, the response of the elevator was obtained by finite element analysis with applied wind forces, as well as the displacement excitations at its supports with the building. To simulate the typical failure modes of elevator in all states, some critical reference velocity‐based envelopes were generated for the safety assessment where the factors of wind velocity, wind direction, and cage level are systematically addressed. The safety envelopes of the building–elevator system were proved to be instinctively efficient for the assessment of its wind resistance under various scenarios. [ABSTRACT FROM AUTHOR]

This study examines whether and how the CEO's time perspective affects corporate green innovation. We document a significant positive relationship between the CEO time perspective and green innovation in Chinese manufacturing-listed firms. Furthermore, consistent with the view that the CEO's time perspective is a significant factor influencing corporate green innovation when resources are plentiful, we find that this positive association is stronger when firms have fewer financial constraints. Overall, our research shows that the CEO's time perspective promotes green innovation in emerging economies. [ABSTRACT FROM AUTHOR]

The neuromorphic computing paradigm, characterized by nonvolatile artificial synapses, is seen as crucial for overcoming the limitations of the von Neumann architecture. Moreover, the effective implementation on a large scale necessitates enhanced performance, reduced energy consumption, and increased robustness of synaptic devices. In this work, a bipartite artificial synapse was developed using Hf0.5Zr0.5O2 (HZO) ferroelectric thin films on Nb:SrTiO3 (NSTO) (110) substrates achieving a substantial storage window and an exceptionally high switching ratio spanning 7 orders of magnitude. Notably, the device demonstrates the capability to transition between multiple resistive states, facilitating multilevel data storage. Additionally, the fabricated device emulates the behavior of biological synapses, exhibiting synaptic plasticity feathers such as long-term potentiation/depression (LTP/LTD), paired-pulse facilitation (PPF), and spike-timing dependent plasticity (STDP). These findings underscore the significant potential of HZO artificial synapse devices for neuromorphic computing applications. [ABSTRACT FROM AUTHOR]

The diagnosis of Talaromyces marneffei infection in HIV-negative patients remains challenging. There is an urgent need for rapid and convenient methods to diagnose this complicated disease. The aim of this study was to evaluate the diagnostic efficiency of metagenomic next-generation sequencing (mNGS) for talaromycosis in non-HIV-infected patients by comparing mNGS with traditional microbial culture. In total, 66 samples from 57 patients were analyzed via both mNGS and microbial culture. The ROC curve showed a sensitivity for mNGS of 97.22%, which was greater than that of microbial culture (61.11%). Samples from the respiratory tract, infectious skin lesions, and lymph nodes are recommended as routine samples for talaromycosis detection via mNGS. Furthermore, mNGS significantly reduced the diagnostic time compared to microbial culture. Overall, our study demonstrated that mNGS is a promising tool for rapid and accurate pathogenic detection in HIV-negative patients with talaromycosis. [ABSTRACT FROM AUTHOR]

Salient object detection (SOD) is designed to mimic human visual mechanisms to identify and segment the most salient part of an image. Although related works have achieved great progress in SOD, they are limited when it comes to interferences of non-salient objects, finely shaped objects and co-salient objects. To improve the effectiveness and capability of SOD, we propose a supervised contrastive learning network with multi-scale interaction and integrity learning named SCLNet. It adopts contrastive learning (CL), multi-reception field confusion (MRFC) and context enhancement (CE) mechanisms. Using this method, the input image is first divided into two branches after two different data augmentations. Unlike existing models, which focus more on boundary guidance, we add a random position mask on one branch to break the continuous of objects. Through the CL module, we obtain more semantic information than appearance information by learning the invariance of different data augmentations. The MRFC module is then designed to learn the internal connections and common influences of various reception field features layer by layer. Next, the obtained features are learned through the CE module for the integrity and continuity of salient objects. Finally, comprehensive evaluations on five challenging benchmark datasets show that SCLNet achieves superior results. Code is available at . [ABSTRACT FROM AUTHOR]

Metal sulfides exhibit good catalytic activity for the oxygen evolution reaction (OER) due to their distinctive electronic and structural properties; however, their inadequate electrical conductivity hinders electron transfer, while easy agglomeration obstructs active sites. Herein, a dual molten salt etching strategy and subsequent sulfidation are employed to anchor CoS/FeS2 heterojunction nanosheets onto an electrically conductive MXene (MXene@CoS/FeS2) for efficient OER. The strong electronic interaction between CoS and FeS2, in conjunction with the conductive MXene, facilitates rapid charge transfer, while the heterostructured nanosheets on the MXene substrate expose abundant catalytic active sites. Dut to its delicate nanostructure, the MXene@CoS/FeS2 composite demonstrates an exceptionally low overpotential (278 mV achieved at 10 mA cm−2) and remarkable stability (over 40 h). Density functional theory (DFT) calculations indicate that the electron transfer at the CoS/FeS2 interface effectively reduces the energy barrier of the rate-determining step (RDS) for MXene@CoS/FeS2. Additionally, the simple and straightforward preparation process eliminates the need for HF, enabling efficient and large-scale catalyst production while ensuring safety and environmental friendliness. Overall, the designed MXene@CoS/FeS2 holds great promise as an OER catalyst. [ABSTRACT FROM AUTHOR]

Plasmonic metal nanostructures can simultaneously scatter and absorb light, with resonance wavelength and strength depending on their morphology and composition. This work demonstrates that unique dichroic effects and high‐contrast colour‐switching can be achieved by leveraging the resonant scattering and absorption of light by plasmonic nanostructures and the specular reflection of the resulting transmitted light. Using core/shell nanostructures comprising a metal core and a dielectric shell, we show that their spray coating on reflective substrates produces dichroic films that can display colour switching at different viewing angles. The high‐contrast colour switching, high flexibility in designing multicolour patterns, and convenience for large‐scale production promise their wide range of applications, including anticounterfeiting, mechanochromic sensing, colour display, and printing. [ABSTRACT FROM AUTHOR]

There is an urgent need for clean recycling strategies to address the increase in food waste (FW) and the harvesting of microalgae (MA). In this study, biogas production potential and operational stability were evaluated by testing combinations of FW and MA mixed at five different ratios. Co-digestion of FW and MA improved substrate biodegradability, achieving a decomposition rate of 0.45/d (FW/MA = 1:1), which is 1.25 to 1.55 times higher than that of MA or FW alone. Co-digestion of FW and MA resulted in a synergistic effect, improving biogas yield by 2.04–26.86%. Four mathematical models were applied to estimate biological degradation and biogas production kinetics, and the Cone model performed better than the other models in terms of reliability and accuracy. The abundance of Bacteroidetes, Firmicutes, and Synergistetes peaked at FW/MA = 1:1. At the same ratio, the genera Methanospirillum, Methanocorpusculum, and Methanomethylovorans were also found to have increased in abundance. The optimal ratio was found to be 1:1 for co-digestion of FW and MA, which is a feasible approach for simultaneous bioenergy production and biomass waste co-disposal. [ABSTRACT FROM AUTHOR]

Activation of the NOD-like receptor protein 3 (NLRP3) inflammasome signaling pathway is an important mechanism underlying myocardial pyroptosis and plays an important role in inflammatory damage to myocardial tissue in patients with cardiovascular diseases (CVDs), such as diabetic cardiomyopathy, ischemia/reperfusion injury, myocardial infarction, heart failure and hypertension. Noncoding RNAs (ncRNAs) are important regulatory factors. Many Chinese medicine (CM) compounds, including their effective components, can regulate pyroptosis and exert myocardium-protecting effects. The mechanisms underlying this protection include inhibition of inflammasome protein expression, Toll-like receptor 4–NF-κB signal pathway activation, oxidative stress, endoplasmic reticulum stress (ERS), and mixed lineage kinase 3 expression and the regulation of silent information regulator 1. The NLRP3 protein is an important regulatory target for CVD prevention and treatment with CM. Exploring the effects of the interventions mediated by CM and the related mechanisms provides new ideas and perspectives for CVD prevention and treatment. [ABSTRACT FROM AUTHOR]

Carbon peaking and carbon neutrality goals have put forward new requirements for the development of sustainable energy. As an important part of the energy system, the power system plays an important role in achieving sustainable energy development. Future power systems will be sustainable, with a high proportion of renewable energy such as photovoltaic and wind power. With advancements in technology and the sustainable development of power systems, the construction costs of wind power, photovoltaics, and other renewable energy sources will continue to decline. At the same time, in order to cope with the uncertainty of renewable energy, more flexible resources need to be built. Under a high proportion of sustainable energy penetration, the utilization rate of flexible resources is low, which will lead to an increase in adjustment costs. Economic issues have an important impact on the development of new power systems, and power system construction will in turn affect social and economic development. Therefore, it is necessary to analyze changing trends in electricity prices in the process of power system sustainability from a quantitative perspective, so as to provide guidance for future planning. In view of the above problems, this paper takes Gansu Province as an example, predicts future power installation trends, by analyzing the power system form evolution, and uses production operation simulation technology to determine the power generation and consumption situation. On this basis, the costs of various power supplies and power grids are calculated, and trends in electricity prices in Gansu Province are forecast. [ABSTRACT FROM AUTHOR]

Background: With the increasing prevalence of colorectal cancer (CRC), optimizing perioperative management is of paramount importance. This study investigates the potential of stellate ganglion block (SGB), known for its stress response-mediating effects, in improving postoperative recovery. We postulate that preoperative SGB may enhance the postoperative recovery of patients undergoing laparoscopic CRC surgery. Methods: We conducted a randomized controlled trial of 57 patients undergoing laparoscopic colorectal cancer surgery at a single center. Patients, aged 18–70 years, were randomly assigned to receive either preoperative SGB or standard care. SGB group patients received 10 mL of 0.2% ropivacaine under ultrasound guidance prior to surgery. Primary outcome was time to flatus, with secondary outcomes encompassing time to defecation, lying in bed time, visual analog scale (VAS) pain score, hospital stays, patient costs, intraoperative and postoperative complications, and 3-year mortality. A per-protocol analysis was used. Results: Twenty-nine patients in the SGB group and 28 patients in the control group were analyzed. The SGB group exhibited a significantly shorter time to flatus (mean [SD] hour, 20.52 [9.18] vs. 27.93 [11.69]; p = 0.012), accompanied by decreased plasma cortisol levels (mean [SD], postoperatively, 4.01 [3.42] vs 7.75 [3.13], p = 0.02). Notably, postoperative pain was effectively managed, evident by lower VAS scores at 6 h post-surgery in SGB-treated patients (mean [SD], 4.70 [0.91] vs 5.35 [1.32]; p = 0.040). Furthermore, patients in the SGB group experienced reduced hospital stay length (mean [SD], day, 6.61 [1.57] vs 8.72 [5.13], p = 0.042). Conclusions: Preoperative SGB emerges as a promising approach to enhance the postoperative recovery of patients undergoing laparoscopic CRC surgery. Clinical trial registration: ChiCTR1900028404, Principal investigator: Xia Feng, Date of registration: 12/20/2019. [ABSTRACT FROM AUTHOR]

[Significance] The proposal of a carbonate factory and its classification study are of great significance for promoting the development of carbonate sedimentology. However, the current classification scheme is not sufficient to cover all carbonate sediments, so it is urgent to clarify different carbonate factories at the level of mechanism and process. [Progress] The carbon used by traditional carbonate factories mainly comes from atmospheric and oceanic inorganic carbon pools, whereas certain marine and continental carbonate factories mainly fix carbon from exogenous carbon pools, so the two are fundamentally different. The latter is often highly significant for tracing deep water environments and climate events. [Conclusions and prospects] As an example, this study takes hydrocarbon seep carbonate, and formally puts forward the concept of a cold seep carbonate factory. In addition, its sedimentary characteristics, biological composition and biogeochemical processes are summarized, and an example is discussed to address its geological significance. The type of carbonate factory based on an exogenous carbon pool deserves much attention. [ABSTRACT FROM AUTHOR]

Background: Endophytes are reported to play a role in resistance against plant pathogens. Understanding the metabolite-mediated endophytic microbiota composition in plants provides insights to improve plant stress resistance. In this study, via metabolome and microbiome analyses, we aimed to elucidate the resistance mechanism of sugarcane cultivars with high resistance to sugarcane pokkah boeng disease (PBD). The endophytic microbial composition and metabolites in the stems of various sugarcane cultivars with high resistance (HR) or high susceptibility (HS) to PBD were analyzed. Results: The results revealed that the endophytic fungi with biocontrol effects such as Shinella, Dechloromonas, and Microbacter were significantly enriched, and the abundance of pathogenic fungi such as Fusarium, Ramichloridium, Scleroramularia, Phaeosphaeriopsis, Sarocladium, Zygophiala, Gibberella, Pseudocercospora, Cyphellophora, Monocillium, Apiotrichum, Microsphaeropsis, and Scleroramularia significantly reduced in the stems of HR cultivars. Additionally, six metabolites [citric acid, isocitrate, malic acid, PC(16:0/0:0), phosphocholine, and lysoPC(16:0)] were significantly related to the endophytes in the stems of HR cultivars. Conclusions: These results suggested that more abundance of antagonistic microbes and highly active metabolic functions of endophytes in the HR cultivars were the important mechanisms underlying their higher resistance to PBD. [ABSTRACT FROM AUTHOR]

Background. Ovarian cancer (OV) is characteristic of high incidence rate and fatality rate in the malignant tumors of female reproductive system. Researches on pathogenesis and therapeutic targets for OV need to be continued. This study mainly analyzed the immune-related pathogenesis and discovered the key immunotherapy targets for OV. Methods. WGCNA was used for excavating hub gene modules and hub genes related to the immunity of OV. Enrichment analysis was aimed to analyze the related pathways of hub gene modules. Biological experiments were used for exploring the effect of hub genes on SKOV3 cells. Results. We identified two hub gene modules related to the immunoscore of OV and found that these genes in the modules were related to the extracellular matrix and viral infections. At the same time, we also discovered six hub genes related to the immunity of OV. Among them, KIF26B and CREB3L1 can affect the proliferation, migration, and invasion of SKOV3 cells by the Wnt/β-catenin pathway. Conclusions. The local infection or inflammation of ovarian may affect the immunity of OV. KIF26B and CREB3L1 are expected to be potential targets for the immunotherapy of OV. [ABSTRACT FROM AUTHOR]

Introduction: Diabetic ulcers have become one of the major complications of diabetes mellitus (DM) and are a leading cause of death and disabling disease. However, current therapies are not effective enough to meet clinical needs. A traditional Chinese medicine (TCM) formula, Pien Tze Huang (PZH), is known as a medicine that is used to treat diabetic ulcers. Methods: In this study, PZH (0.05 g/cm2 and 0.15 g/cm2) and the positive drugrhEGF were topically administered in a high-fat diet (HFD) and streptozotocin (STZ)-induced diabetic full-thickness incisional wounds, respectively. Wound healing was assessed by wound closure rate, two-photon microscope (SHG), staining with Hematoxylin and eosin (H&E), and Masson's trichrome (MTC). Then, RNA sequencing (RNA-seq) analysis, Enzyme-linked immunosorbent assay (ELISA), western blotting, and immunofluorescence (IF), network analysis, were performed. Results and discussion: The results showed that PZH significantly accelerated wound healing, as well as enhanced the expression of collagen. RNA-seq analysis showed that PZH has functions on various biological processes, one of the key biological processes is inflammatory response. Tlr9, Klrk1, Nod2, Tlr2, and Ifng were identified as vital targets and the NF-κB signaling pathway was identified as the vital pathway. Additionally, PZH profoundly reduced the levels of Cleaved caspase-3 and promoted the expression of CD31 and TGF-ß1. Mechanically, PZH significantly decreased expression of NKG2-D, NOD2, and TLR2, and further inhibited the activation of downstream NF-κB signaling pathway and inhibited expression of inflammatory factors (IFN-γ and IL-1ß). Importantly, we found that several active ingredients may play a significant role in diabetic wound healing, including Notoginsenoside R1, Deoxycorticosterone, Ursolic acid, and 4-Methoxyphenol. In summary, our study sheds light on the complicated mechanisms underlying the promising anti-diabetic wounds of PZH and provides the discovery of agents treating diabetic ulcers. [ABSTRACT FROM AUTHOR]

Root metabolites and soil microbial community structure in the rhizosphere play critical roles in crop growth. Here, we assessed the efficiency of conventional and tissue culture propagation methods in modulating the soil health and microbiota in the rhizosphere of sugarcane (Saccharum officinarum L.) plants. The seeding canes were obtained using newly planted and two‐year ratooned canes propagated by conventional (CSN and CSR) or tissue culture (TCN and TCR) methods. Changes in soil fertility, root metabolites and soil microbial community structure in the rhizosphere of sugarcane plants obtained using these canes were assessed. The activities of soil β‐glucosidase and aminopeptidase, soil microbial biomass nitrogen, and abundances of soil beneficial microbes, both at phyla and genera levels, were significantly higher in the rhizosphere of sugarcane plants in TCN and TCR treatments than those in that of plants in CSN and CSR treatments. Furthermore, flavonoid and flavonol biosynthesis and alanine, aspartate and glutamate metabolism were significantly upregulated in the roots of TCR and TCN plants compared with those in the roots of CSN and CSR plants. These results suggest that the tissue culture propagation method is a sustainable method for sugarcane cultivation to improve soil fertility and health in sugarcane rhizosphere. [ABSTRACT FROM AUTHOR]

Chinese Folk Drum music is an excellent traditional cultural resource, it has brilliant historical and cultural heritage and excellent traditional cultural connotation. However, the survey found that the social and cultural values, tourism economic values, and national self-confidence embodied in folk drum music, such as Xi'an drum music, are far from being released, and even its own inheritance and development are facing difficulties. The research focuses on the automatic generation of Xi'an drum music, with the aim of further inheriting, developing, and utilizing this exceptional traditional cultural resource. While Artificial Intelligence (AI) music generation has gained popularity in recent years, most platforms primarily focus on modern music rather than Chinese folk music. To address these issues and the unique challenges faced by Xi'an drum music, this paper proposes a Bi-LSTM network-based deep reinforcement learning model. The model incorporates the distinctive characteristics of ancient Chinese music, such as pitch, chord, and mode, and utilizes the Actor-Critic algorithm in reinforcement learning. During the simulation generation stage, an improved method of generating strategies through reward and punishment scores is introduced. Additionally, the model takes into account abstract concept constraints, such as chord progression and music theory rules, which are translated into computer language. By constructing a chord reward mechanism and a music principle reward mechanism, the model achieves harmony constraints and enables the systematic generation of drum music. Experimental results demonstrate that the proposed model, based on Bi-LSTM deep reinforcement learning, can generate Xi'an drum music with high quality and artistic aesthetics. This research contributes to the preservation, development, and utilization of Xi'an drum music, leveraging advancements in AI music generation technology. [ABSTRACT FROM AUTHOR]

The full design of a liquid neutron reflectometer for China spallation neutron source is reported. The horizontal sample geometry enables studies on both solid and liquid samples. A neutron beam with a wavelength range from 1 to 10 Å and neutron flux intensity of 4 × 107 n/cm2/s was optimized for the classical specular reflection. A multi-blade detector with high detection efficiency and a high neutron counting rate was used to ensure the performance of neutron detection. Data acquisition and reduction routines were demonstrated to confirm the reliability of the measurements. The design and algorithms developed in this study pave the way for the future construction of liquid reflectometry for high-performance neutron scattering measurements. [ABSTRACT FROM AUTHOR]

Background: Gut microbiome dysbiosis has been implicated in various gastrointestinal and extra-gastrointestinal diseases, but evidence on the efficacy and safety of fecal microbiota transplantation (FMT) for therapeutic indications remains unclear. Methods: The gutMDisorder database was used to summarize the associations between gut microbiome dysbiosis and diseases. We performed an umbrella review of published meta-analyses to determine the evidence synthesis on the efficacy and safety of FMT in treating various diseases. Our study was registered in PROSPERO (CRD42022301226). Results: Gut microbiome dysbiosis was associated with 117 gastrointestinal and extra-gastrointestinal. Colorectal cancer was associated with 92 dysbiosis. Dysbiosis involving Firmicutes (phylum) was associated with 34 diseases. We identified 62 published meta-analyses of FMT. FMT was found to be effective for 13 diseases, with a 95.56% cure rate (95% CI: 93.88-97.05%) for recurrent Chloridoids difficile infection (rCDI). Evidence was high quality for rCDI and moderate to high quality for ulcerative colitis and Crohn's disease but low to very low quality for other diseases. Conclusion: Gut microbiome dysbiosis may be implicated in numerous diseases. Substantial evidence suggests FMT improves clinical outcomes for certain indications, but evidence quality varies greatly depending on the specific indication, route of administration, frequency of instillation, fecal preparation, and donor type. This variability should inform clinical, policy, and implementation decisions regarding FMT. [ABSTRACT FROM AUTHOR]

Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer. HCC with liver fluke infection could harbor unique biological behaviors. This study was aimed at investigating radiomics features of HCC with liver fluke infection and establishing a model to predict the expression of cytokeratin 7 (CK7) and cytokeratin 19 (CK19) as well as prognosis at the same time. A total of 134 HCC patients were included. Gadoxetic acid-enhanced magnetic resonance imaging (MRI) images of all patients were acquired. Radiomics features of the tumor were extracted and then data dimensionality was reduced. The radiomics model was established to predict liver fluke infection and the radiomics score (Radscore) was calculated. There were 11 features in the four-phase combined model. The efficiency of the combined model increased significantly compared to each single-phase MRI model. Radscore was an independent predictor of liver fluke infection. It was also significantly different between different expression of CK7/ CK19. Meanwhile, liver fluke infection was associated with CK7/CK19 expression. A cut-off value was set up and all patients were divided into high risk and low risk groups of CK7/CK19 positive expression. Radscore was also an independent predictor of these two biomarkers. Overall survival (OS) and recurrence free survival (RFS) of negative liver fluke infection group were significantly better than the positive group. OS and RFS of negative CK7 and CK19 expression were also better, though not significantly. Positive liver fluke infection and CK19 expression prediction groups harbored significantly worse OS and RFS, survival of positive CK7 expression prediction was unsatisfying as well. A radiomics model was established to predict liver fluke infection among HCC patients. This model could also predict CK7 and CK19 expression. OS and RFS could be foreseen by this model at the same time. [ABSTRACT FROM AUTHOR]

The formation of technical standards is a complex process that involves the integration of various technical resources and organizational resources. It is influenced by multiple factors. This paper focuses on four independent variables, namely market size, economic environment, infrastructure construction, and government support, which are regarded as different dimensions of the socio-technical landscape. Additionally, R&D investment is introduced as a mediator variable to construct a model that examines the role of the socio-technical landscape in the formation of technical standards. Empirical tests are conducted using relevant data from the 4G technology in China's mobile communication industry. The study reveals that there is a positive causal relationship between market size, economic environment, infrastructure development, government support, and technology standards. Furthermore, it finds that R&D investment plays a significant mediating role between the economic environment and technology standards, infrastructure development and technology standards, and government support and technology standards. These findings provide valuable insights for enterprises and governments in promoting the formation of technical standards. Plain Language Summary: The formation of technical standards is a complex process that involves the integration of various technical resources and organizational resources. It is influenced by multiple factors. This paper focuses on four independent variables, namely market size, economic environment, infrastructure construction, and government support, which are regarded as different dimensions of the socio-technical landscape. Additionally, R&D investment is introduced as a mediator variable to construct a model that examines the role of the socio-technical landscape in the formation of technical standards. Empirical tests are conducted using relevant data from the 4G technology in China's mobile communication industry. The study reveals that there is a positive causal relationship between market size, economic environment, infrastructure development, government financial support, and technology standards. Furthermore, it finds that R&D investment plays a significant mediating role between the economic environment and technology standards, infrastructure development and technology standards, and government financial support and technology standards. These findings provide valuable insights for enterprises and governments in promoting the formation of technical standards. [ABSTRACT FROM AUTHOR]

Biochar is considered to be the most promising substrate for the preparation of environmentally functional materials. The modification of bamboo char can significantly improve the removal rate of toxic ions from wastewater; however, there are few reports that focus on the radiation grafting method. Here, glyceride methacrylate (GMA) is successfully grafted onto bamboo char through electron beam radiation, followed by amination using the existing epoxide group in diethyltriamine, and finally, treated with hydrochloric acid to obtain protonated diethyltriamine-functionalized bamboo char (CDGBC). The results of IR, TG, XRD, and SEM prove the successful fabrication of a CDGBC biosorbent. The results show that the solution pH has a great effect on the adsorption capacity, and a maximum adsorption capacity of 169.13 mg/g is obtained at pH = 2 for Cr(VI). In addition, the adsorption behavior of Cr(VI) onto CDGBC is demonstrated to obey the pseudo-second-order kinetic and Freundlich isotherm models, and thermodynamic analysis exhibits that Cr(VI) adsorption is an endothermic spontaneous process. A possible adsorption mechanism based on the electrostatic interaction, reduction, and surface complexation is proposed, according to the obtained results. This work confirms that radiation-induced grafting modification can effectively transform biochar into a high-performance adsorbent for Cr(VI) removal, offering a new approach to synthesizing an efficient biosorbent. [ABSTRACT FROM AUTHOR]

Purpose: Immune-related pathways actively participate in the progression of schizophrenia (SCZ), however, roles of immune-related miRNAs in SCZ are still unclear. Methods: A microarray expression study was conducted to explored roles of immune-related genes in SCZ. Functional enrichment analysis by using "clusterProfiler" was used to identify molecular alterations of SCZ. Protein-protein interaction (PPI) network was constructed and helped core molecular factors identification. Based on The Cancer Genome Atlas (TCGA) database, clinical significances of hub immune-related genes in cancers were also been explored. Then, correlation analyses were used to determine immune-related miRNAs. We further validated that hsa-miR-1299 could be an effective diagnostic biomarker for SCZ via analyzing multi-cohorts' data and quantitative real-time PCR (qRT-PCR). Results: A total of 455 mRNAs and 70 miRNAs that were differentially expressed between SCZ and control samples. Functional enrichment analysis based on differentially expressed genes (DEGs) hinted that immune-related pathways were significantly correlated with SCZ. Furthermore, a total of 35 immune-related genes that involved in disease onset and showed significant co-expressed relationships. Hub immune-related gene CCL4 and CCL22 are valuable in tumor diagnosis and survival prediction. Furthermore, we also identified 22 immune-related miRNAs that play important roles in this disease. An immune-related miRNAs-mRNAs regulatory network was constructed to provide miRNAs regulatory roles in SCZ. Core miRNAs expression status of hsa-miR-1299 were also validated in another cohort, which suggested its diagnostic performance for SCZ. Conclusions: Our study reports the downregulation of some miRNAs in the process of SCZ are important. Shared genomics characteristics between SCZ and cancers also provide novel insights for cancers. A significant alteration of hsa-miR-1299 expression is effective as biomarker for the diagnosis of SCZ, suggesting that this miRNA could be a specific biomarker. [ABSTRACT FROM AUTHOR]

Ni2In-type hexagonal compounds MM′X (M, M′ = transition metals, X = main element) involving magnetostructural transition (MST) have attracted much attention due to their giant magnetocaloric effect (MCE). Physical pressure, as an effective method, has been used to adjust the MST and the resultant MCE. Enhanced and diminished MCEs by hydrostatic pressure were both reported previously, but the underlying mechanism is unclear. Here, we report our neutron diffraction study on MST and MCE regulated by hydrostatic pressure in MnNi1 – xFexSi1 – yGey alloys. Careful refinements indicate that the martensitic phase shows a linear ferromagnetic structure with spin moment confined on Mn sites, which remains almost unchanged at pressures lower than 5 kbar even though slight compressions of Mn–Mn bond lengths can be identified. The MST keeps sharp under pressures lower than 5 kbar, while the derived volume change (ΔV/V) across MST reduces 7%, i.e., from 2.84% (0 kbar) to 2.63% (2.1 kbar), due to the effect of pressure on two-phase coexistence. Accordingly, the estimated lattice entropy change (ΔSLatt) based on the Debye approximation reduces by 10% from 37.1 J/kg K (0 kbar) to 33.5 J/kg K (2.1 kbar). These ΔSLatt values, driven by temperature, are all somewhat larger than the magnetic entropy change driven by a 5 T magnetic field. This result may imply that a magnetic field of 5 T is not sufficient for the MST to complete. As the pressure reaches 5 kbar, the MST notably slows down. This may originate from the extended temperature region of two-phase coexistence but not the decoupling of MST. [ABSTRACT FROM AUTHOR]

In practical production, cane stems with buds are generally used as seed for propagation. However, long-terms cane stems only easily lead to some problems such as disease sensitivity, quality loss, etc. Recently, cane seedings, which are produced by tissue culture were used in sugarcane production, but few studies on cane health related to tissue culture seedings. Therefore, to evaluate the immunity and health of sugarcanes growing from different reproduction modes, the endophytic microbial compositions in cane roots between stem and tissue culture seedlings were analyzed using high-throughput techniques. The results showed that the endophytic microbial compositions in cane roots were significant differences between stem and tissue culture seedlings. At the genus level, Pantoea, Bacillus, Streptomyces, Lechevalieria, Pseudomonas, Nocardioides, unclassified_f__Comamonadaceae enriched as the dominant endophytic bacterial genera, and Rhizoctonia, Sarocladium, Scytalidium, Wongia, Fusarium, unclassified_f__Phaeosphaer, unclassified_c__Sordariom, unclassified_f__Stachybot, Poaceascoma, Microdochium, Arnium, Echria, Mycena and Exophiala enriched as the dominant endophytic fungal genera in cane roots growing from the tissue culture seedlings. In contrast, Mycobacterium, Massilia, Ralstonia, unclassified_f__Pseudonocardiacea, norank_f__Micropepsaceae, Leptothrix and Bryobacter were the dominant endophytic bacterial genera, and unclassified_k__Fungi, unclassified_f__Marasmiaceae, Talaromyces, unclassified_c__Sordariomycetes and Trichocladium were the dominant endophytic fungal genera in cane roots growing from stem seedlings. Additionally, the numbers of bacterial and fungal operational taxonomic units (OTUs) in cane roots growing from tissue culture seedlings were significantly higher than those of stem seedlings. It indicates that not only the endophytic microbial compositions in cane roots can be shaped by different propagation methods, but also the stress resistance of sugarcanes can be improved by the tissue culture propagation method. [ABSTRACT FROM AUTHOR]

Vitiligo is an autoimmune disease of the skin that targets pigment-producing melanocytes and results in patches of depigmentation that are visible as white spots. Recent research studies have yielded a strong mechanistic understanding of this disease. Fructus Ligustri Lucidi (FLL) has been used for premature graying of hair since ancient China and is currently used to treat vitiligo. However, the key biomarkers and mechanisms underlying FLL in vitiligo remain unclear. This study aimed to identify the potential biomarkers and mechanisms of FLL in vitiligo using network pharmacology analysis.Methods: The expression profiles of GSE65127 and GSE75819 were downloaded from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) between the vitiligo and healthy samples. Gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment of DEGs were performed using R analyses. We performed R to further understand the functions of the critical targets. Cytoscape tools have facilitated network topology analysis. Molecular docking was performed using Auto Dock Vina software.Results: The results showed that 13 DEGs were screened in vitiligo. Based on bioinformatics, network pharmacology and Western blot, we found that the critical targets of melanoma antigen recognized by 5,6-dihydroxyindole-2-carboxylic acid oxidase (TYRP1) may be related to the mechanism of action of FLL in the treatment of vitiligo.Conclusion: TYRP1, as a melanocyte molecular biomarker, may be closely related to the underlying mechanism of FLL in the treatment of vitiligo via the inhibition of melanocyte death. [ABSTRACT FROM AUTHOR]

Proton pump inhibitors (PPIs) are the most used acid-inhibitory drugs, with a wide range of applications in the treatment of various digestive diseases. However, recently, there has been a growing number of digestive complications linked to PPIs, and several studies have indicated that the intestinal flora play an important role in these complications. Therefore, developing a greater understanding of the role of the gut microbiota in PPI-related digestive diseases is essential. Here, we summarize the current research on the correlation between PPI-related digestive disorders and intestinal flora and establish the altered strains and possible pathogenic mechanisms of the different diseases. We aimed to provide a theoretical basis and reference for the future treatment and prevention of PPIrelated digestive complications based on the regulation of the intestinal microbiota. [ABSTRACT FROM AUTHOR]

Modular multi‐level converters (MMCs) are a mainstay in many HVDC transmission projects worldwide. Half‐bridge MMC (HB‐MMC) is mostly used in the current project. The large capacitor of sub modules (SMs) is often required in MMC to ensure the low voltage ripple. Here, the three‐stage switching MMC (TSS‐MMC) topology is proposed, which can greatly reduce the capacitor voltage ripple of SMs by dynamically adjusting the position of inductance on the upper and down bridge arms. The principle of reducing capacitor voltage ripple based on equal capacitance theory is introduced. The selection of the inductance value of each part in TSS‐MMC is introduced. The start‐up process strategy, capacitor voltage ripple reduction strategy in steady‐state operation, and DC fault ride‐through strategy of TSS‐MMC are proposed. The effectiveness and engineering practicability of the proposed TSS‐MMC is verified by transient simulation program built on PSCAD/EMTDC. The simulation results show that compared with MMC with circulating current suppressing controller (CCSC), it can effectively reduce the capacitor voltage ripple by over 50%. Furthermore, the total used inductance of TSS‐MMC can also be reduced. A comprehensive assessment of TSS‐MMC is also carried out. [ABSTRACT FROM AUTHOR]

Our study aimed to explore the correlation between age at smoking initiation and smoking cessation for the risk for stroke in China. We investigated 50,174 participants from one of the urban areas of China Kadoorie Biobank (CKB) Study. Hazard ratios (HRs) and 95% confidence intervals (95% CIs) for association between smoking and incidence of stroke were estimated using Cox regression model. During a median of 10.7 years of follow-up, 4370 total stroke cases were documented. Among men, comparing current smokers to never smokers, the HR of total stroke for current smokers was 1.279 (95% CI, 1.134–1.443) for total stroke. The HRs of total stroke were 1.344 (1.151–1.570) for those started smoking at age less than 20 years, 1.254 (1.090–1.443) for those started smoking at age 20–30 years, and 1.205 (1.012–1.435) for those started smoking at age 30 year and above, with a dose-response relation (P for trend, 0.004). Comparing former smokers to current smokers, in the low pack-year group, those stopped smoking at age less than 65 years had a 18.2% decreased risk for total stroke (0.818; 0.673–0.994). The decreased risk was not found in those stopped smoking at age 65 years and above. Similar results were observed in the high pack-year group. In conclusion, we found that current smokers had a higher stroke risk than never smokers, and the risk increased with a younger age at smoking initiation. Smoking cessation can reduce the risk for stroke, especially could benefit from cessation at a younger age. [ABSTRACT FROM AUTHOR]

Introduction: The incidence of cholangiocarcinoma (CCA) has increased worldwide in recent years. Given the poor prognosis associated with the current management approach of CCA, new therapeutic agents are warranted to improve the prognosis of this patient population. Methods: In this study, we extracted five cardiac glycosides (CGs) from natural plants: digoxin, lanatoside A, lanatoside C, lanatoside B, and gitoxin. Follow-up experiments were performed to assess the effect of these five extracts on cholangiocarcinoma cells and compounds with the best efficacy were selected. Lanatoside C (Lan C) was selected as the most potent natural extract for subsequent experiments. We explored the potential mechanism underlying the anticancer activity of Lan C on cholangiocarcinoma cells by flow cytometry, western blot, immunofluorescence, transcriptomics sequencing, network pharmacology and in vivo experiments. Results: We found that Lan C time-dependently inhibited the growth and induced apoptosis of HuCCT-1 and TFK-1 cholangiocarcinoma cells. Besides Lan C increased the reactive oxygen species (ROS) content in cholangiocarcinoma cells, decreased the mitochondrial membrane potential (MMP) and resulted in apoptosis. Besides, Lan C downregulated the protein expression of STAT3, leading to decreased expression of Bcl-2 and Bcl-xl, increased expression of Bax, activation of caspase-3, and initiation of apoptosis. N-acetyl-L-cysteine (NAC) pretreatment reversed the effect of Lan C. In vivo, we found that Lan C inhibited the growth of cholangiocarcinoma xenografts without toxic effects on normal cells. Tumor immunohistochemistry showed that nude mice transplanted with human cholangiocarcinoma cells treated with Lan C exhibited decreased STAT3 expression and increased caspase-9 and caspase-3 expression in tumors, consistent with the in vitro results. Conclusion: In summary, our results substantiates that cardiac glycosides have strong anti-CCA effects. Interestingly the biological activity of Lan C provides a new anticancer candidate for the treatment of cholangiocarcinoma. [ABSTRACT FROM AUTHOR]

Background: Endometriosis (EMs), the ectopic planting of functional endometrium outside of the uterus, is a leading cause of infertility and pelvic pain. As a fundamental mRNA modification, N6-methyladenosine (m6A) participates in various pathological processes. However, the role of m6A RNA modification in endometriosis remains unclear. The present study explores METTL3-mediated m6A modification and the mechanisms involved in endometriosis. Methods: The dominant m6A regulators in EMs were analysed using RT‒PCR. Candidate targets and possible mechanisms of METTL3 were assessed by m6A-mRNA epitranscriptomic microarray and RNA sequencing. A primary ESCs model was employed to verify the effect of METTL3 on m6A modification of SIRT1 mRNA, and the mechanism was elucidated by RT‒PCR, Western blotting, MeRIP, and RIP assays. CCK-8 viability assays, Transwell invasion assays, EdU proliferation assays, wound healing migration assays, and senescence-associated β-galactosidase staining were performed to illuminate the potential biological mechanism of METTL3 and SIRT1 in ESCs in vitro. An in vivo PgrCre/ + METTL3 −/− female homozygous mouse model and a nude mouse xenograft model were employed to further investigate the physiologic consequences of METTL3-mediated m6A alteration on EMs. Results: Our data show that decreased METTL3 expression significantly downregulates m6A RNA methylation levels in ESCs. Silencing m6A modifications mediated by METTL3 accelerates ESCs viability, proliferation, migration, and invasion in vitro. The m6A reader protein YTHDF2 binds to m6A modifications to induce the degradation of SIRT1 mRNA. SIRT1/FOXO3a signalling pathway activation is subsequently inhibited, promoting the cellular senescence of ESCs and inhibiting the ectopic implantation of ESCs in vitro and in vivo. Conclusions: Our findings demonstrate that METTL3-mediated m6A methylation epigenetically regulates the ectopic implantation of ESCs, resulting in the progression of endometriosis. Our study establishes METTL3-YTHDF2-SIRT1/FOXO3a as a critical axis and potential mechanism in endometriosis. [ABSTRACT FROM AUTHOR]

The Viséan–Serpukhovian boundary is poorly defined in South China, hampering regional and global stratigraphical correlations. The foraminiferal and conodont distribution of the Baping Formation in the carbonate-slope Danlu section permits the recognition of an interval from the middle Viséan to the uppermost Serpukhovian in a continuous succession. The base of the Serpukhovian in Danlu is recognized by the first occurrences of Janischewskina delicata , Howchinia subplana and questionable ' Millerella ' tortula. At a slightly younger level, the conodont Lochriea ziegleri is first recorded. A calibration on the first occurrence of L. ziegleri in different basins at a global scale has been revised compared to auxiliary markers within the ammonoids and foraminifers. The late occurrence of L. ziegleri in the Danlu section also supports a lack of synchronicity in the global first occurrence of this taxon. This study calls for the recognition of a new base for the Serpukhovian under a far better correlation between different zonal schemes and fossil groups. [ABSTRACT FROM AUTHOR]

The TENO-family schemes (Fu et al. in J Comput Phys 305: 333–359, 2016) have been demonstrated to perform well for compressible gas dynamics and turbulent flow predictions on structured meshes. However, the extension of the TENO schemes to unstructured meshes is non-trivial and challenging, particularly when the multiple design objectives are pursued simultaneously, i.e., restoring the high-order accuracy in smooth regions, retaining the low numerical dissipation for small-scale features, maintaining the sharp shock-capturing property, and featuring the good numerical robustness for high-Mach flows. In this work, a family of very-high-order (up to seventh-order accuracy) robust finite-volume TENO schemes with dual ENO-like stencil selection for unstructured meshes is proposed. The stencils include one large stencil and several small stencils. The novelty originates from a so-called dual ENO-like stencil selection strategy. Following a strong scale separation, the ENO-like stencil selection procedure with a small C T is first enforced among all the candidates such that the high-order candidate scheme on the large stencil is adopted for the final reconstruction when the local flow is smooth. If the large stencil is judged to be crossed by discontinuities, a second ENO-like stencil selection with a relatively large C T is applied to all the left small stencils and the ENO property is obtained by selecting the smooth small stencils which are not crossed by discontinuities. The smaller C T in the first stage ensures that the high-order reconstruction is restored for smooth flow scales with higher wavenumbers. On the other hand, the larger C T in the second stage can enforce a strong nonlinear adaptation for capturing discontinuities with better robustness. Such a dual ENO-like stencil selection strategy introduces an explicit scale separation and deploys the optimal strategy for different types of flows correspondingly. Without parameter tuning, a set of benchmark simulations has been conducted to validate the performance of the proposed TENO schemes. Numerical results demonstrate the good numerical robustness and the low-dissipation property for highly compressible flows with shockwaves. [ABSTRACT FROM AUTHOR]

Background: Acute kidney injury (AKI) is common after cardiac interventional procedures. The prevalence and clinical outcome of AKI in patients with acute myocardial infarction (AMI) after undergoing intra-aortic balloon pump (IABP) implantation remains unknown. The aim of this study was to investigate the incidence, risk factors, and prognosis of AKI in specific patient populations. Methods: We retrospectively reviewed 319 patients with AMI between January 2017 and December 2021 and who had successfully received IABP implantation. The diagnostic and staging criteria used for AKI were based on guidelines from "Kidney Disease Improving Global Outcomes". The composite endpoint included all-cause mortality, recurrent myocardial infarction, rehospitalization for heart failure, and target vessel revascularization. Results: A total of 139 patients (43.6%) developed AKI after receiving IABP implantation. These patients showed a higher incidence of major adverse cardiovascular events (hazard ratio [HR]: 1.55, 95% confidence interval [CI]: 1.06-2.26, p = 0.022) and an increased risk of all-cause mortality (HR: 1.62, 95% CI: 1.07-2.44, p = 0.019). Multivariable regression models found that antibiotic use (odds ratio [OR]: 2.07, 95% CI: 1.14-3.74, p = 0.016), duration of IABP use (OR: 1.24, 95% CI: 1.11-1.39, p < 0.001) and initial serum creatinine (SCr) (OR: 1.01, 95% CI: 1.0-1.01, p = 0.01) were independent risk factors for AKI, whereas emergency percutaneous coronary intervention was a protective factor (OR: 0.35, 95% CI: 0.18-0.69, p = 0.003). Conclusions: AMI patients who received IABP implantation are at high risk of AKI. Close monitoring of these patients is critical, including the assessment of renal function before and after IABP implantation. Additional preventive measures are needed to reduce the risk of AKI in these patients. [ABSTRACT FROM AUTHOR]

Aging is a biological process of progressive deterioration of physiological functions, which poses a serious threat to individual health and a heavy burden on public health systems. As population aging continues, research into antiaging drugs that prolong life and improve health is of particular importance. In this study, the polysaccharide from stems and leaves of Chuanminshen violaceum was obtained with water extraction and alcohol precipitation, and then separated and purified with DEAE anion exchange chromatography and gel filtration to obtain CVP-AP-I. We gavaged natural aging mice with CVP-AP-I and performed serum biochemical analysis, histological staining, quantitative realtime PCR (qRT-PCR) and ELISA kit assays to analyze inflammation and oxidative stress-related gene and protein expression in tissues, and 16SrRNA to analyze intestinal flora. We found that CVP-AP-I significantly improved oxidative stress and inflammatory responses of the intestine and liver, restored the intestinal immune barrier, and balanced the dysbiosis of intestinal flora. In addition, we revealed the potential mechanism behind CVP-AP-I to improve intestinal and liver function by regulating intestinal flora balance and repairing the intestinal immune barrier to regulate the intestinal-liver axis. Our results indicated that C. violaceum polysaccharides possessed favorable antioxidant, anti-inflammatory and potentially anti-aging effects in vivo. [ABSTRACT FROM AUTHOR]

The trade-off and coordinated development of the "production-living-ecological" functions (PLEFs) of an area is an important basis for the optimization of the spatial pattern of the land, and helps to promote the harmonious symbiotic relationship between human beings and nature. This paper combines ecological niche theory, a coupling coordination model, and a trade-off synergy model to construct an evaluation index system for Chinese county PLEFs. Quantitative methods were used to measure spatiotemporal evolution characteristics, trade-off synergy of PLEFs in 38 counties in Chongqing, China, and the coupling coordination degree between PLEFs. The results showed that the ecological niche width of the "production-ecological" function revealed an overall growth trend. However, there was a mismatch in regional development of the "production-ecological" function, showing dislocation characteristics of "high in the west and low in the east" and "high in the east and low in the west." The niche width of the life function is similar to the comprehensive niche width of PLEFs, showing the characteristics of fluctuation and partition change. PLEFs and both the aforementioned functions showed distribution characteristics of "high in the west and low in the east," with the whole moving towards the stage of coordinated coupling, of which the "production-living" function has the highest coupling level. The functional coupling coordination degree of "production-living-ecological" is generally manifested as "high in the west and low in the east," and changing from the primary stage of imbalance to well-coordinated development. The "production-ecological" and "living-ecological" functions are in low-level imbalance in the primary and moderate coordination stages. Additionally, the evolution trend of the "production-ecological" and the "living-ecological" functions are similar, showing alternating and fluctuating development characteristics. Overall, in the past 20 years, Chongqing's "living-production" function has changed from a trade-off model to a collaborative development relationship, and the "living-ecological" function is generally based on a collaborative development relationship. The "ecological function" is manifested as a trade-off constraint relationship. Moreover, the coordinated development level of "living-production," "living-ecological," and "production-ecological" functions in the central urban area has been greatly improved, while counties have gradually shown different degrees of trade-offs. [ABSTRACT FROM AUTHOR]

Dentin sialophosphoprotein (DSPP) is primarily expressed by differentiated odontoblasts (dentin-forming cells), and transiently expressed by presecretory ameloblasts (enamel-forming cells). Disease-causing DSPP mutations predominantly fall into two categories: 5' mutations affecting targeting and trafficking, and 3' − 1 frameshift mutations converting the repetitive, hydrophilic, acidic C-terminal domain into a hydrophobic one. We characterized the dental phenotypes and investigated the pathological mechanisms of DsppP19L and Dspp−1fs mice that replicate the two categories of human DSPP mutations. In DsppP19L mice, dentin is less mineralized but contains dentinal tubules. Enamel mineral density is reduced. Intracellular accumulation and ER retention of DSPP is observed in odontoblasts and ameloblasts. In Dspp−1fs mice, a thin layer of reparative dentin lacking dentinal tubules is deposited. Odontoblasts show severe pathosis, including intracellular accumulation and ER retention of DSPP, strong ubiquitin and autophagy activity, ER-phagy, and sporadic apoptosis. Ultrastructurally, odontoblasts show extensive autophagic vacuoles, some of which contain fragmented ER. Enamel formation is comparable to wild type. These findings distinguish molecular mechanisms underlying the dental phenotypes of DsppP19L and Dspp−1fs mice and support the recently revised Shields classification of dentinogenesis imperfecta caused by DSPP mutations in humans. The Dspp−1fs mice may be valuable for the study of autophagy and ER-phagy. [ABSTRACT FROM AUTHOR]

The rapid drop in internal temperature of mass concrete can readily lead to temperature cracks. Hydration heat inhibitors reduce the risk of concrete cracking by reducing the temperature during the hydration heating phase of cement-based material but may reduce the early strength of the cement-based material. Therefore, in this paper, the influence of commercially available hydration temperature rise inhibitors on concrete temperature rise is studied from the aspects of macroscopic performance and microstructure characteristics, and their mechanism of action is analyzed. A fixed mix ratio of 64% cement, 20% fly ash, 8% mineral powder and 8% magnesium oxide was used. The variable was different admixtures of hydration temperature rise inhibitors at 0%, 0.5%, 1.0% and 1.5% of the total cement-based materials. The results showed that the hydration temperature rise inhibitors significantly reduced the early compressive strength of concrete at 3 d, and the greater the amount of hydration temperature rise inhibitors, the more obvious the decrease in concrete strength. With the increase in age, the influence of hydration temperature rise inhibitor on the compressive strength of concrete gradually decreased, and the decrease in compressive strength at 7 d was less than that at 3 d. At 28 d, the compressive strength of the hydration temperature rise inhibitor was about 90% in the blank group. XRD and TG confirmed that hydration temperature rise inhibitors delay early hydration of cement. SEM showed that hydration temperature rise inhibitors delayed the hydration of Mg(OH)2. [ABSTRACT FROM AUTHOR]