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Background: Cerebrospinal fluid (CSF) analysis is essential for diagnosing various disorders affecting the central nervous system (CNS). Traditionally, CSF cell count analysis is performed manually using a Neubauer chamber hemocytometer, which is labor-intensive and prone to subjective interpretation. Methods: In this study, we evaluated the analytical and clinical performance of the Microscanner C3, an automated cell counting system, for CSF analysis using artificially prepared samples and 150 clinical CSF samples. Results: The lowest detectable white blood cell (WBC) count was 3.33 cells/µL, and the lowest detectable red blood cell (RBC) count was 3.67 cells/µL. The coefficients of variation (CV%) for the Microscanner C3 were lower than those for the Neubauer chamber at all cell concentrations. The correlation coefficients (R) between the Microscanner C3 and conventional methods were high: 0.9377 for WBCs and 0.9952 for RBCs when compared with the Neubauer chamber, and 0.8782 for WBCs and 0.9759 for RBCs when compared with the flow cytometer. Additionally, the Microscanner C3 showed good agreement with both the Neubauer chamber and flow cytometer in the Passing–Bablok regression analysis and Bland–Altman analysis for WBC count at all concentrations and RBC count at concentrations of 0–1000 cells/µL. Conclusions: The Microscanner C3 proved to be more sensitive, precise, and consistent compared to the conventional hemocytometer. The new system is also compact, convenient, and cost-effective, making it a valuable option for clinical laboratories. [ABSTRACT FROM AUTHOR]

This study explores the impact of milling methods on the quality and noodle-making performance by comparing jet-milled (WF-JM) and ultra-centrifugally milled (WF-UM) purple-colored whole wheat flours. WF-JM exhibits smaller starch granules and a fragmented protein matrix attributed to the increased milling pressure. Physicochemical analyses reveal lower moisture and higher damaged starch in WF-JM. Rheological analyses show lower viscosity in the WF-JM blends. The mixograph results reveal weaker dough-mixing stability and strength for WF-JM. Cooked noodles from WF-JM are uneven, in contrast to uniform WF-UM strands. Blending WF-UM enhances noodle quality. Overall, the noodle-making performance for WF-JM was inferior compared to WF-UM, confirming the significantly negative impact of damaged starch and fragmented protein matrix in whole wheat flour than the positive effect of particle size. This study highlights the complex interplay between milling methods, particle size, and physicochemical attributes, providing insights for optimizing whole wheat flour processing and product quality. [ABSTRACT FROM AUTHOR]

In global P2P accommodation platform operations, hosts can experience severe stress from the platform company, customers, and technological complexity. Applying sociotechnical theory, this study aims to explore platform stress in the sharing economy and verify the stressor-strain-outcome (SSO) framework. A survey of Airbnb hosts demonstrates that 'conflict with headquarters,' 'negative reviews from customers,' and 'technological complexity' influence the 'strain' of hosts. Furthermore, we verify the role of firm performance that negatively affects switching intention. It also serves as a moderator in the relationship between strain and switching intention. Focusing on platform stress, this study evaluates the concept and definition of platform stress and provides useful insights for platform business operators. [ABSTRACT FROM AUTHOR]

Mono or bis(tetrazole–thiolato) Pd(II) or Pt(II) complexes were obtained from the reactions of dialkyl Pd(II) or Pt(II) complexes with organic tetrazole–thiones (1-aryl- or 1-alkyl-1H-tetrazole-5-thiones) via deprotonation. In contrast, equimolar reactions of zerovalent Pt(0) or Pd(0) complexes with organic tetrazole–thiones afforded hydrido or bis(tetrazole–thiolato) Pt(II) and Pd(II) complexes, and cyclometallated Pt(II) or Pd(II) complexes bearing a tetrazole–thiolato moiety via oxidative addition, depending on the organic substituents on the tetrazole–thiones. In particular, variable (time and temperature)-dependent 1H-NMR spectra of the hydrido Pt(II) tetrazole–thiolates reveal an upfield shift of the hydride signal, suggesting N,S-coordination behavior of the tetrazole–thiolato ligand. Additionally, the N-CH2 signal corresponds to the six-membered ring of platinacycle or palladacycle exhibiting geminal coupling with multiple protons and PR3 ligands; these coupling values were further determined using 1H{31P} experiments. Finally, treatment of the alkyl Pd(II) tetrazole–thiolate or Pd(II) bis(tetrazole–thiolates) with organic tert-butyl isocyanide, thiophenol, and organic halides caused the selective insertion of the isocyanide into the Pd–C bond or deprotonation to afford a Pd(II) disulfide complex and substitution to afford new organic tetrazolyl sulfides. [ABSTRACT FROM AUTHOR]

Most triblock copolymer-based physical hydrogels form three-dimensional networks through micellar packing, and formation of polymer loops represents a topological defect that diminishes hydrogel elasticity. This effect can be mitigated by maximizing the fraction of elastically effective bridges in the hydrogel network. Herein, we report hydrogels constructed by complexing oppositely charged multiblock copolymers designed with a sequence pattern that maximizes the entropic and enthalpic penalty of micellization. These copolymers self-assemble into branched and bridge-rich network units (netmers), instead of forming sparsely interlinked micelles. We find that the storage modulus of the netmer-based hydrogel is 11.5 times higher than that of the micelle-based hydrogel. Complementary coarse grained molecular dynamics simulations reveal that in the netmer-based hydrogels, the numbers of charge-complexed nodes and mechanically reinforcing bridges increase substantially relative to micelle-based hydrogels. In the formation of physical hydrogels from triblock copolymers, formation of polymer loops can result in topological defects. Here, the authors report the development of hydrogels from oppositely charged copolymers which assembled to form branched and bridge-rich networks. [ABSTRACT FROM AUTHOR]

Introduction: The emergence of novel infectious diseases has amplified the urgent need for effective prevention strategies, especially ones targeting vulnerable populations such as children. Factors such as the high incidence of both emerging and existing infectious diseases, delays in vaccinations, and routine exposure in communal settings heighten children's susceptibility to infections. Despite this pressing need, a comprehensive exploration of research trends in this domain remains lacking. This study aims to address this gap by employing text mining and modeling techniques to conduct a comprehensive analysis of the existing literature, thereby identifying emerging research trends in infectious disease prevention among children. Methods: A cross‐sectional text mining approach was adopted, focusing on journal articles published between January 1, 2003, and August 31, 2022. These articles, related to infectious disease prevention in children, were sourced from databases such as PubMed, CINAHL, MEDLINE (Ovid), Scopus, and Korean RISS. The data underwent preprocessing using the Natural Language Toolkit (NLTK) in Python, with a semantic network analysis and topic modeling conducted using R software. Results: The final dataset comprised 509 journal articles extracted from multiple databases. The study began with a word frequency analysis to pinpoint relevant themes, subsequently visualized through a word cloud. Dominant terms encompassed "vaccination," "adolescent," "infant," "parent," "family," "school," "country," "household," "community," "HIV," "HPV," "COVID‐19," "influenza," and "diarrhea." The semantic analysis identified "age" as a key term across infection, control, and intervention discussions. Notably, the relationship between "hand" and "handwashing" was prominent, especially in educational contexts linked with "school" and "absence." Latent Dirichlet Allocation (LDA) topic modeling further delineated seven topics related to infectious disease prevention for children, encompassing (1) educational programs, (2) vaccination efforts, (3) family‐level responses, (4) care for immunocompromised individuals, (5) country‐specific responses, (6) school‐based strategies, and (7) persistent threats from established infectious diseases. Conclusion: The study emphasizes the indispensable role of personalized interventions tailored for various child demographics, highlighting the pivotal contributions of both parental guidance and school participation. Clinical Relevance: The study provides insights into the complex public health challenges associated with preventing and managing infectious diseases in children. The insights derived could inform the formulation of evidence‐based public health policies, steering practical interventions and fostering interdisciplinary synergy for holistic prevention strategies. [ABSTRACT FROM AUTHOR]

This cross-sectional study aimed to assess the interrelationships between PM2.5 exposure, heavy metal concentrations, and oxidative stress indicators, while evaluating the impact of antioxidant intake, such as vitamins, on these associations. PM2.5 exposure assessments were conducted using portable sensor-based monitors; biomarker analyses for heavy metals and oxidative stress were performed in 114 non-smoking adults. We observed that personal or ambient PM2.5 exposure levels were not associated with increased levels of heavy metals in blood and urine, nor with oxidative stress levels in urine. However, the concentrations of cadmium and lead in blood, and those of chromium and nickel in urine, were significantly associated with the urinary malondialdehyde (MDA) concentration. Additionally, increases in blood cadmium, urinary chromium, and nickel levels were significantly associated with higher urinary MDA concentrations in the non-vitamin-supplement group, but this trend was not observed in the regular vitamin supplement group. Our findings suggest that a regular intake of vitamin supplements might modulate the relationship between heavy metal exposure and oxidative stress, indicating potential protective effects against oxidative damage induced by PM2.5 and heavy metals. This study highlights the complexity of environmental pollutant exposure and its impact on human health, emphasizing the need for further research to elucidate the underlying mechanisms and explore potential protective strategies. [ABSTRACT FROM AUTHOR]

The COVID-19 pandemic has highlighted the urgent need for rapid and accurate diagnostic methods for various infectious diseases, including SARS-CoV-2. Traditional RT-PCR methods, while highly sensitive and specific, require complex equipment and skilled personnel. In response, we developed an integrated RT-LAMP-MS assay, which combines rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) with microscanning (MS) technology for detecting SARS-CoV-2. The assay uses magnesium pyrophosphate formed during LAMP amplification as a visual marker, allowing direct observation via microscopy without the need for additional chemical indicators or probes. For the SARS-CoV-2/IC RT-LAMP-MS assay, the sample-LAMP reagent mixture was added to a microchip with SARS-CoV-2 primers and internal controls, then incubated at 62 °C for 30 min in a heat block, followed by amplification analysis using a microscanner. In clinical tests, the RT-LAMP-MS assay showed 99% sensitivity and 100% specificity, which is identical to the RT-LAMP results and comparable to the commercial AllplexTM SARS-CoV-2 assay results. Additionally, the limit of detection (LOD) was determined to be 10−1 PFU mL−1 (dynamic range: 103~10−1 PFU mL−1). The assay delivers results in 30 min, uses low-cost equipment, and demonstrates 100% reproducibility in repeated tests, making it suitable for point-of-care use in resource-limited settings. [ABSTRACT FROM AUTHOR]

2D MoS2 has gained attention for the post‐silicon material owing to its atomically thin nature and dangling bond‐free surface. The bi‐layer MoS2 is considered a promising material for electronic devices due to its better electrical properties than monolayer MoS2. However, the uniform growth of bi‐layer MoS2 is still challenging. Herein, the uniform growth of bi‐layer MoS2 is demonstrated using gas‐phase alkali metal‐assisted metal–organic chemical vapor deposition (GAA‐MOCVD). Thanks to enhanced metal reactant diffusion length in GAA‐MOCVD, the uniform growth of bi‐layer MoS2 film is achieved even at fast nucleation kinetics for a shorter growth time compared to previously reported MOCVD. The bi‐layer MoS2 field‐effect transistors (FETs) show superior electrical properties such as sheet conductance and electron mobility than monolayer MoS2 FETs. The electron mobility of bi‐layer MoS2 FETs with bismuth contacts reaches a maximum of 92.35 cm2 V−1 s−1. Using the partially grown epitaxial bi‐layer (PGEB) MoS2, it is demonstrated that a photodetector showed a near‐infrared photoresponse with a low dark current that is advantageous for both monolayer and bi‐layer applications. The potential expansion of the growth technique to layer‐by‐layer growth can result in boosted performance across a wide spectrum of electronic and optoelectronic devices employing MoS2. [ABSTRACT FROM AUTHOR]

Real‐world applications of graphene require reliable routes for synthesizing large‐area graphene and methods to tune its structural and electrical properties. A viable and facile route is presented for synthesizing 3D porous graphene (3PG) on PI films with tunable patterns and locations via the photon‐pen writing technique and subsequent oxygen plasma treatment. The laser power and scan speed are optimized for 3PG synthesis. The electrical properties and surface hydrophilicity are controlled via surface customization of 3PG with optimal oxygen plasma treatment duration and laser inter‐spacing. The capability of humidity sensors based on surface‐customized 3PG with hydrophobic surface is demonstrated. [ABSTRACT FROM AUTHOR]

With the advent of 5G and the Internet of Things (IoT), there is a high demand for lightweight and flexible electromagnetic interference (EMI) shielding membranes with multifunctional capabilities. However, the rapid advancement of technology has led to environmental deterioration, emphasizing the significance of eco‐friendly and sustainable materials for EMI shielding. In this study, a novel environment‐friendly EMI shielding membrane with electric thermotherapy function and antibacterial properties is developed. The membrane substrate utilizes recycled polyethylene terephthalate (rPET) and is sequentially coated with synthesized FeCo@C nanoparticles (NPs) and silver nanowires (Ag NWs). The membrane is then dip‐coated with PDMS to modify the surface superhydrophobicity. Experimental results affirm that the developed environment‐friendly EMI shielding membrane possesses exceptional shielding efficiency (68 dB) and can be effectively utilized for safe and low‐voltage thermotherapy (137 °C by 2 V) while exhibiting antibacterial properties. [ABSTRACT FROM AUTHOR]

Solid‐state lithium (Li) metal batteries (LMBs) have been developed as a promising replacement for conventional Li‐ion batteries due to their potential for higher energy. However, the current solid‐state electrolytes used in LMBs have limitations regarding mechanical and electrochemical properties and interfacial stability. Here, a fluorine (F)‐containing solid polymer electrolyte (SPE) having a bi‐continuous structure of F‐containing elastomers and plastic crystals is reported. The trifluoroethyl acrylate‐based SPE (T‐SPE) exhibits high ionic conductivity over 10−3 S cm−1, superior mechanical elasticity, and robust LiF‐rich interphases at both the Li metal anode and the LiNi0.83Mn0.06Co0.11O2 cathode. Full cells with thin T‐SPEs and low negative/positive capacity ratios below 0.5 at the high‐operating voltage of 4.5 V demonstrate a high specific energy of 538 Wh kganode+cathode+electrolyte−1 and maintain 393 Wh kg−1 at a high specific power of 804 W kganode+cathode+electrolyte−1. The F‐containing phase‐separated SPE system provides a powerful strategy for achieving high‐energy and ‐power solid‐state LMBs. [ABSTRACT FROM AUTHOR]

Influenza viruses cause highly contagious respiratory diseases that cause millions of deaths worldwide. Rapid detection of influenza viruses is essential for accurate diagnosis and the initiation of appropriate treatment. We developed a loop-mediated isothermal amplification and lateral flow assay (LAMP-LFA) capable of simultaneously detecting influenza A and influenza B. Primer sets for influenza A and influenza B were designed to target conserved regions of segment 7 and the nucleoprotein gene, respectively. Optimized through various primer set ratios, the assay operated at 62 °C for 30 min. For a total of 243 (85 influenza A positive, 58 influenza B positive and 100 negative) nasopharyngeal swab samples, the performance of the influenza A/B multiplex LAMP-LFA was compared with that of the commercial AllplexTM Respiratory Panel 1 assay (Seegene, Seoul, Korea). The influenza A/B multiplex LAMP-LFA demonstrated a specificity of 98% for the non-infected clinical samples, along with sensitivities of 94.1% for the influenza A clinical samples and 96.6% for the influenza B clinical samples, respectively. The influenza A/B multiplex LAMP-LFA showed high sensitivity and specificity, indicating that it is reliable for use in a low-resource environment. [ABSTRACT FROM AUTHOR]

The task of assigning internationally accepted commodity codes (aka HS codes) to traded goods is a critical function of customs offices. Like court decisions made by judges, this task follows the doctrine of precedent and can be nontrivial even for experienced officers. Together with the Korea Customs Service (KCS), we propose a first-ever explainable decision supporting model that suggests the most likely subheadings (i.e., the first six digits) of the HS code. The model also provides reasoning for its suggestion in the form of a document that is interpretable by customs officers. We evaluated the model using 5,000 cases that recently received a classification request. The results showed that the top-3 suggestions made by our model had an accuracy of 93.9% when classifying 925 challenging subheadings. A user study with 32 customs experts further confirmed that our algorithmic suggestions accompanied by explainable reasonings, can substantially reduce the time and effort taken by customs officers for classification reviews. [ABSTRACT FROM AUTHOR]

We have investigated the optical and magneto-optical properties of monolayer (ML) WSe2 on flakes of β-Ga2O3 under high magnetic fields. Remarkably, sharp emission peaks were observed and associated with localized excitons related to point defects. A detailed study of low-temperature photoluminescence (PL) and magneto-PL under high perpendicular magnetic field up to 9 T was carried out. Several sharp emission peaks have shown valley g-factors values close to −4, which is an unusual result for localized excitons in WSe2. Furthermore, some PL peaks have shown higher g-factor values of ≈ −7 and ≈ −12, which were associated with the hybridization of strain localized dark excitons and defects. The reported results suggest that β-Ga2O3 is, indeed, a promising dielectric substrate for ML WSe2 and also to explore fundamental physics in view of possible applications in quantum information technology. [ABSTRACT FROM AUTHOR]

A shielding experiment using activation detectors was performed at the CERN High-energy AcceleRator Mixed-field (CHARM) facility and the CERN Shielding Benchmark Facility (CSBF). The protons (24 GeV/c) were bombarded into a 50-cm-thick copper target, and the released neutrons were transmitted through various shields located vertically upward from the target. Ordinary concrete slabs of 40- to 160-cm thicknesses and steel slabs of 20- to 80-cm thicknesses were installed by changing the material and the thickness of the shield. Activation detectors of bismuth, indium, aluminum, and graphite were variously placed in these shields and activated by high energy neutrons. From the radionuclide production rate in the activation detectors, the attenuation profiles through the various shielding materials were obtained for the reactions of 209Bi(n,xn)210-xBi(x = 4–9), 115In(n,n')115mIn, 27Al(n,α)24Na, and 12C(n,2n)11C. Estimated attenuation lengths of high energy neutrons through concrete and steel were compared with cited data and discussed. Monte Carlo simulations using FLUKA, PHITS, and GEANT4 were also performed as benchmark calculations, and they agreed with the experimental data, generally within a factor of 2. [ABSTRACT FROM AUTHOR]

Objective: To describe the hyperproliferation of Elschnig pearl‐type posterior capsule opacification and concurrent uveitis in two canine eyes after phacoemulsification, followed by spontaneous resolution of the Elschnig pearls. Animal Studied: A 10‐year‐old castrated male Spitz (Case 1) and a 4‐year‐old spayed female Bichon Frise (Case 2). Procedure: Elschnig pearls proliferating beyond the anterior capsulotomy site were observed in the right eye 10 months after bilateral diabetic cataract surgery (Case 1) and 7 months after unilateral cataract surgery (Case 2). In both cases, hyperproliferation occurred where the anterior capsule did not overlap with the intraocular lens (IOL), and was accompanied by aqueous flare. In Case 1, the pearls extended from the anterior capsule and adhered to the iris, causing focal posterior synechia. No other possible causes of uveitis were apparent. Results: Initially, uveitis severity improved after the administration of topical and systemic anti‐inflammatory drugs. However, uveitis recurred when the dosage of anti‐inflammatory treatment was reduced. The Elschnig pearls underwent morphological changes throughout the follow‐up period. In both cases, the pearls beyond the anterior capsulotomy resolved spontaneously after 5 months. Only a few pearls remained between the IOL and posterior capsule, and no recurrence of pearl proliferation was observed at the last follow‐up. Conclusions: To the best of our knowledge, this is the first report of spontaneous Elschnig pearl regression in dogs. Lens‐induced uveitis (LIU) may have been caused by anterior chamber hyperproliferative pearls. LIU associated with hyperproliferative pearls may be managed with appropriate anti‐inflammatory treatment and monitoring. [ABSTRACT FROM AUTHOR]

This research aims to investigate the relationship between prior spending and unplanned purchases based on an understanding of decision framing effects and compensatory psychology. We argue that the impact of prior buying on unplanned purchases is moderated by the decision frame (separate vs. integrated). We conducted a series of four sequential scenario-based experimental studies to support our theoretical predictions. Consequently, our study revealed that travelers within a separate decision frame exhibited a stronger compensatory motive, leading to a higher unplanned purchase when prior spending was low. In practice, this research has managerial implications using compensatory consumption marketing strategies. [ABSTRACT FROM AUTHOR]

To achieve high-performance solid-state lithium (Li) metal batteries (LMBs), both the Li-ion conductivity and mechanical robustness of the polymeric electrolytes are crucial. Herein, we develop a series of elastomeric electrolytes with different phase structures to optimize their Li-ion conductivity and mechanical properties. The phase structures are controlled by modifying the side alkyl chain length of the acrylate monomer during the in situ polymerization process. Specifically, when using short alkyl chain monomers such as methyl and ethyl, a homogeneous phase of the electrolyte was generated after the in situ polymerization. On the other hand, longer-chain monomers like butyl and hexyl result in bicontinuous and macrophase-separated structures, respectively. Among these different structures, the elastomeric electrolyte based on butyl acrylate (BA) and succinonitrile (SN), denoted as BA-SPE, exhibits excellent ionic conductivity (>1 mS cm−1) and high mechanical extensibility due to its bicontinuous structure. The BA-SPE also demonstrates stable operation when used in a Li/LiNi0.8Co0.1Mn0.1O2 (NCM811) full cell. To further enhance the performance of the BA-SPE, we introduced a dual salt system comprising lithium bis(trifluoromethane sulfonyl)imide (LiTFSI) and lithium difluoro(oxalato)borate (LiDFOB). This modification resulted in superior full cell performance, with 87% capacity retention after 100 cycles under a current density of 0.5 mA cm−2, using a 40 µm-Li anode and high-loading NCM811 cathode (>10 mg cm−2). [ABSTRACT FROM AUTHOR]

Germanium diselenide (GeSe2) is a 2D semiconductor with air stability, a wide bandgap, and anisotropic optical properties. The absorption and photoluminescence (PL) of single‐crystalline 2D GeSe2 grown by metal‐organic chemical vapor deposition and their dependence on temperature and polarization are studied. The PL spectra exhibit peaks at 2.5 eV (peak A) and 1.8 eV (peak B); peak A displays a strongly polarized emission along the short axis of the crystal, and peak B displays a weak polarization perpendicular to that of peak A. With increasing temperature, peak B shows anomalous behaviors, i.e., an increasing PL energy and intensity. The excitation energy‐dependent PL, time‐resolved PL, and density functional theory calculations suggest that peak A corresponds to the band‐edge transition, whereas peak B originates from the inter‐band mid‐gap states caused by selenium vacancies passivated by oxygen atoms. The comprehensive study on the PL of single‐crystalline GeSe2 sheds light on the origins of light emission in terms of the band structure of anisotropic GeSe2, making it beneficial for the corresponding optoelectronic applications. [ABSTRACT FROM AUTHOR]

Establishing a multifunctional solid electrolyte interphase (SEI), which can not only reduce the electrode–electrolyte side reactions, but also promote the mass transport behavior of Li, can promisingly stabilize the operation of lithium metal batteries (LMBs). Herein, we introduce a multifunctional SEI constructing Cu based three-dimensional host (MSEI@Cu), whose electrochemical and chemical SEIs are characterized by excellent ionic conductivity, Li diffusivity and mechanical stability. The fabrication of MSEI@Cu is realized by a novel ultra-fast double coating strategy, during which metallic Cu nanowires grown on Cu foam are covered by a double coating layer constituted by a dense surficial carbon layer and inner carbon matrices containing CuSO4 and In2S3. Through electrochemical or chemical reduction by Li, CuSO4 and In2S3 are converted to Li2S and LixIn, and the positively charged residual Cu atoms can promote the decomposition of TFSi− anions, contributing to abundant formation of LiF. As a result, the inner SEI formed on MSEI@Cu is enriched by Li2S, LixIn and LiF, all of which synergistically improve electrical resistivity, mechanical strength and mass transport kinetics of Li. Meanwhile, the amorphous carbon matrices evenly distributing the above compounds further enhance the mechanical integrity of the as-formed SEI by successfully accommodating volume changes. Importantly, all the above components are densely covered by an outmost surficial carbon layer, which prevents the direct exposure of conductive metallic Cu nanowires to the electrolyte, thereby reducing excessive electrolyte decomposition. Benefiting from the sophistically engineered advanced SEI, MSEI@Cu exhibited substantial improvements in electrochemical performance: when paired with a LiFePO4 cathode, MSEI@Cu delivered a specific capacity of 125.9 mA h g−1 at 1C with an impressive capacity retention of 80% for 500 cycles. [ABSTRACT FROM AUTHOR]

Isozymes are enzymes that catalyze identical biological reactions, yet exhibit slight variations in structures and catalytic efficiency, which enables the precise adjustment of metabolism to fulfill the specific requirements of a particular tissue or stage of development. Methionine aminopeptidase (MetAP) isozymes function a critical role in cleaving N-terminal methionine from nascent proteins to generate functional proteins. In humans, two distinct MetAP types I and II have been identified, with type I further categorized into cytosolic (MetAP1) and mitochondrial (MetAP1D) variants. However, despite extensive structural studies on both bacterial and human cytosolic MetAPs, the structural information remains unavailable for human mitochondrial MetAP. This study was aimed to elucidate the high-resolution structures of human mitochondrial MetAP1D in its apo-, cobalt-, and methionine-bound states. Through a comprehensive analysis of the determined structures and a docking simulation model with mitochondrial substrate peptides, we present mechanistic insights into the cleavage process of the initiator methionine from mitochondrial proteins. Notably, despite the shared features at the active site between the cytosolic and mitochondrial MetAP type I isozymes, we identified distinct structural disparities within the active-site pocket primarily contributed by two specific loops that could play a role in accommodating specific substrates. These structural insights offer a basis for the further exploration of MetAP isozymes as critical players in cellular processes and potential therapeutic applications. [ABSTRACT FROM AUTHOR]

Japanese encephalitis (JE) is a very severe disease characterized by high fatality rates and the development of permanent behavioral, psychiatric, and neurological sequelae among survivors. Japanese encephalitis virus (JEV), a flavivirus, is responsible for JE. In Asia, Genotype I (GI) has emerged as the dominant strain, replacing Genotype III (GIII). However, no clinically approved drug is available to treat JEV infection, and currently available commercial vaccines derived from JEV GIII strains provide only partial protection against GI. Utilizing a reverse genetics system, this study attempted to produce a novel chimeric JEV strain with high efficacy against JEV GI. Accordingly, a GI/GIII intertypic recombinant strain, namely SA14-GI env, was generated by substituting the E region of the GIII SA14-14-2 strain with that of the GI strain, K05GS. The neurovirulence of the mutant virus was significantly reduced in mice. Analysis of the immunogenicity of the chimeric virus revealed that it induced neutralizing antibodies against JEV GI in mice, and the protective efficacy of SA14-GI env was higher than that of SA14-14-2. These findings suggest that SA14-GI env may be a safe and effective live-attenuated vaccine candidate against JEV GI. [ABSTRACT FROM AUTHOR]

Networking of molecular hosts via non-covalent interactions has become one of the central topics in crystal engineering. We herein report the self-assembly of silver(I) coordination polymers with an NS4-macrocycle (L) whose connectivity patterns depend on anion types. First, a one-pot reaction of L with AgPF6 yielded an endo/exocyclic one-dimensional (1D) coordination polymer {[Ag3L2](PF6)3·2CH2Cl2}n (1) in which two silver(I) atoms (endoAg)2 are sandwiched by two L ligands to form an L(endoAg)2L unit. Further, the disilver(I) sandwich units are linked by one exocyclic silver(I) atom (exoAg), giving rise to a polymeric loop chain array of 1 without anion coordination. When L was reacted with AgCF3SO3 and AgCF3CO2, again, endo/exocyclic 1D coordination polymers {[Ag4L2(CF3SO3)2](CF3SO3)2·CH2Cl2}n (2) and {[Ag4L2(CF3CO2)2](CF3CO2)2·4CH3OH}n (3) with a similar connectivity pattern were obtained, respectively. In both 2 and 3, one endoAg atom and one exoAg atom link three L ligands to form a double-stranded array with one anion coordinated to the exoAg atom. In the anion exchange experiments, the formation of 2 (CF3SO3− form) is relatively favourable over 1 (PF6− form) or 3 (CF3CO2− form) mainly due to the anion access-friendly conformation and moderate binding affinity of CF3SO3− which can easily gain entry. [ABSTRACT FROM AUTHOR]

A benzothiazole-coupled NS4-macrocycle derivative L was synthesized via a three-step route for the detection of Hg2+. Chemosensor L, as a dual channel (UV-vis and fluorescence) receptor, undergoes a significant hypsochromic shift and fluorescence quenching in the presence of Hg(ClO4)2 in ethanol/DMSO (v/v, 9 : 1), showing remarkable selectivity towards Hg2+ over other metal ions both in single and competitive metal ion experiments. The observed chelation-enhanced quenching (CHEQ) effect seems to be attributed to the combination of the heavy atom effect and Hg2+⋯benzothiazole interaction. However, such Hg2+ sensing responses in both channels were not observed for non-perchlorate anions. To explain the anion-dependent Hg2+ sensing, systematic studies on the complexations of L with Hg(ClO4)2 and HgI2 were performed via UV-vis/fluorescence titrations, comparative NMR, and anion exchange. Taken together, the Hg2+ sensing critically depends on the Hg–Ntert bonding which triggers the hypsochromic shift or the CHEQ effect. A weaker coordinating ClO4− allows an endocyclic complex to form with the Hg–Ntert bond being responsive, while a stronger coordinating I− leads to exocyclic complexation without the Hg–Ntert bond being non-responsive. The crystal structure of the mercury(II) iodide complex [Hg2(L)I4] featuring an exocyclic dinuclear form also supports this explanation. This result could present a new perspective on the design of macrocycle-based chemosensors. [ABSTRACT FROM AUTHOR]

The dipole characteristics of Frenkel excitons and charge-transfer excitons between donor and acceptor molecules in organic heterostructures such as exciplexes are important in organic photonics and optoelectronics. For the bilayer of the organic donor 4,4′,4′′-tris[(3-methylphenyl)phenylamino]triphenylamine and acceptor 2,4,6-tris(biphenyl-3-yl)-1,3,5-triazine molecules, the exciplexes form aligned dipoles perpendicular to the Frenkel excitons, as observed in back focal plane photoluminescence images. The angular chromism of exciplexes observed in the 100 meV range indicates possible delocalization and angle-sensing photonic applications. The blue shift of the peak position and increase in the linewidth of photoluminescene spectra with increasing excitation power are caused by the repulsive aligned exciplex dipole moments with a long lifetime (4.65 μs). Electroluminescence spectra of the exciplex from organic light-emitting diodes using the bilayer are blue-shifted with increasing bias, suggesting unidirectional alignment of the exciplex dipole moments. The observation of exciplex dipole moment alignments across molecular interfaces can facilitate the controlled coupling of exciton species and increase efficiency of organic light-emitting diodes. The directional features of dipole moments of Frenkel excitons and exciplexes are important for optoelectronic applications. Here, the authors study photo- and electro-luminescence emissions of organic heterostructures and observe angular chromism of exciplexes, indicating possible delocalization. [ABSTRACT FROM AUTHOR]

A 10‐year‐old spayed female Maltese dog was presented for hypotony of the right eye (OD) with decreased vision. The dorsomedial region of conjunctiva was partially swollen but the globe remained intact. Slit‐lamp biomicroscopy revealed intraocular haemorrhage, uveitis, and a limbal, corneoconjunctival ulcer near the swollen conjunctiva. During ocular ultrasonography (OUS), the sclera became indented and hypotony occurred, implicating a full‐thickness scleral wound. Ultrasound biomicroscopy (UBM) confirmed a full‐thickness scleral defect, which was surgically apposed. The intraocular pressure normalised and the uveitis subsided post‐operatively. OUS and UBM were helpful in suggesting and definitively diagnosing scleral perforation, leading to accurate treatment and good prognosis. [ABSTRACT FROM AUTHOR]

Machine learning approaches using satellite imagery are providing accessible ways to infer socioeconomic measures without visiting a region. However, many algorithms require integration of ground-truth data, while regional data are scarce or even absent in many countries. Here we present our human-machine collaborative model which predicts grid-level economic development using publicly available satellite imagery and lightweight subjective ranking annotation without any ground data. We applied the model to North Korea and produced fine-grained predictions of economic development for the nation where data is not readily available. Our model suggests substantial development in the country's capital and areas with state-led development projects in recent years. We showed the broad applicability of our model by examining five of the least developed countries in Asia, covering 400,000 grids. Our method can both yield highly granular economic information on hard-to-visit and low-resource regions and can potentially guide sustainable development programs. A human-AI collaborative computer vision algorithm produces grid-level economic statistics using satellite images and lightweight human annotation, revealing granular development patterns in North Korea and five other least developed Asian countries. [ABSTRACT FROM AUTHOR]

Due to the different crystallization methods, two Hg(II) complexes of a 19-membered NO2S2-macrocycle (L) and its oxidized ligand (HLox), exhibiting different stoichiometries, were prepared. First, mercury(II) iodide reacts with L to afford a dinuclear metallacycle complex [Hg2(L)2I4] (1) in which the mercury(II) exists outside the macrocyclic cavity. Meanwhile, the slow diffusion reaction gave an unusual pseudo-square planar-induced mercury(II) complex, which shows three separated parts with the formula [Hg2(HLox)I5]2[HgI2] (2). There are two complex cation units that are exo-coordinated, along with one unit consisting of a metal cluster anion. Surprisingly, L was oxidized in the disulfoxidized form (HLox) in this condition. NMR titration was used to monitor both the structural and binding characteristics of the complex formed between L and mercury(II) iodide in a solution. [ABSTRACT FROM AUTHOR]

The acquisition of personal information has been generally accepted in the pandemic situation as an effective measure to prevent infection, while at the same time raising concerns regarding the infringement of personal privacy. The current study aimed to propose and empirically test a research model for restaurant customers on the disclosure of personal information in a pandemic situation. Privacy calculus theory and institutional theory were applied to theoretically explain the drivers/inhibitors and behavioral responses that affect disclosure of personal information. We verified that the most influential factor on intention to disclose was "perceived benefit", followed by "government pressure" as another strong predictor. We present theoretical and practical implications for restaurant managers and policy agencies. [ABSTRACT FROM AUTHOR]

This research examines the impact of lockdown relaxation on tourists' and residents' movements in the context of restaurant patronage in Jeju Island, South Korea. Drawing on spatial interaction and multi-attribute attitude theories, we hypothesized the difference between tourists and residents in terms of restaurant visit patterns and choices during lockdown relaxation. A multi-method approach was adopted: spatial, data-driven analysis, and experiments. Lockdown relaxation is found to affect how tourists and residents choose and visit restaurants in different ways. This research provides explanations about tourists' and residents' mobility and guidelines that practitioners can use to prepare for "living with COVID-19." [ABSTRACT FROM AUTHOR]

Boron nitride nanotubes (BNNTs) were purified without the use of a dispersant by controlling the surface tension and steric repulsion of solvent molecules. This method effectively enhanced the difference in solubilities of impurities and BNNTs. The purification process involved optimizing the alkyl-chains of alcohol solvents and adjusting the concentration of alcohol solvent in water to regulate surface tension and steric repulsion. Among the solvents tested, a 70 wt% t-butylalcohol in water mixture exhibited the highest selective isolation of BNNTs from impurities based on differences in solubilities. This favorable outcome was attributed to the surface tension matching with BNNTs, steric repulsion from bulky alkyl chain structures, and differences in interfacial energy between BNNT–liquid and impurity–liquid interfaces. Through this optimized purification process, impurities were removed to an extent of up to 93.3%. Additionally, the purified BNNTs exhibited a distinct liquid crystal phase, which was not observed in the unpurified BNNTs. [ABSTRACT FROM AUTHOR]

High‐performance chiroptical synaptic phototransistors are successfully demonstrated using heterojunctions composed of a self‐assembled nanohelix of a π‐conjugated molecule and a metal oxide semiconductor. To impart strong chiroptical activity to the device, a diketopyrrolopyrrole‐based π‐conjugated molecule decorated with chiral glutamic acid is newly synthesized; this molecule is capable of supramolecular self‐assembly through noncovalent intermolecular interactions. In particular, nanohelix formed by intertwinded fibers with strong and stable chiroptical activity in a solid‐film state are obtained through hydrogen‐bonding‐driven, gelation‐assisted self‐assembly. Phototransistors based on interfacial charge transfer at the heterojunction from the chiroptical nanohelix to the metal oxide semiconductor show excellent chiroptical detection with a high photocurrent dissymmetry factor of 1.97 and a high photoresponsivity of 218 A W−1. The chiroptical phototransistor demonstrates photonic synapse‐like, time‐dependent photocurrent generation, along with persistent photoconductivity, which is attributed to the interfacial charge trapping. Through the advantage of synaptic functionality, a trained convolutional neural network successfully recognizes noise‐reduced circularly polarized images of handwritten alphabetic characters with better than 89.7% accuracy. [ABSTRACT FROM AUTHOR]

Solid-state structural transformation is an interesting methodology used to prepare various metal–organic frameworks (MOFs) that are challenging to prepare in direct synthetic procedures. On the other hand, solid-state [2 + 2] photoreactions are distinctive methodologies used for light-driven solid-state transformations. Meanwhile, most of these photoreactions explored are quantitative in nature, in addition to them being stereo-selective and regio-specific in manner. In this work, we successfully synthesized two photoreactive novel binuclear Zn(II) MOFs, [Zn2(spy)2(tdc)2] (1) and [Zn2(spy)4(tdc)2] (2) (where spy = 4-styrylpyridine and tdc = 2,5-thiophenedicarboxylate) with different secondary building units. Both MOFs are interdigitated in nature and are 2D and 1D frameworks, respectively. Both the compounds showed 100% and 50% photoreaction upon UV irradiation, as estimated from the structural analysis for 1 and 2, respectively. This light-driven transformation resulted in the formation of 3D, [Zn2(rctt-ppcb)(tdc)2] (3), and 2D, [Zn2(spy)2(rctt-ppcb)(tdc)2] (4) (where rctt = regio, cis, trans, trans; ppcb = 1,3-bis(4′-pyridyl)-2,4-bis(phenyl)cyclobutane), respectively. These solid-state structural transformations were observed as an interesting post-synthetic modification. Overall, we successfully transformed novel lower-dimensional frameworks into higher-dimensional materials using a solid-state [2 + 2] photocycloaddition reaction. [ABSTRACT FROM AUTHOR]

Procurement of health information systems (HIS) is a complex and critical task that requires early identification of interoperability requirements. However, specifying adequate requirements is often associated with several challenges. We examined relevant peer-reviewed literature and public documents (policy documents, annual reports, and newspapers) to summarize existing challenges in specifying interoperability requirement during procurement of HISs. In this study, 32 public documents and 2343 peer-reviewed articles were found using Google search engine, Springer, PubMed and ScienceDirect. Collected data were analyzed using a thematic coding schema. Our result shows that challenges related to describing the needs properly, conflicting needs and knowledge gaps are shared between most articles. Further research in the direction of developing a model that can bridge knowledge gaps, facilitate interdisciplinary collaboration, and help to avoid fuzzy requirements is needed. [ABSTRACT FROM AUTHOR]

In recent decades, contemporary art practices have expanded into social processes as art, and even to educational experiments. Artists create participatory projects combining art with educational activities and goals. Despite the prevalence of these art works, contemporary art literature continues to focus primarily on the artist, thereby displacing the experiences of participants. Hence, from the stance of an art educator, I carried out qualitative multiple case studies to examine the learning experiences of the participants as well as the pedagogical frameworks of the artists. On-site observations and individual interviews were carried out with the artists, core group members who directly collaborated with the artists, and public audience members of three participatory, socially engaged art works. Through a cross-case thematic analysis, the findings demonstrate the value of intrinsically motivated learning enabled by a learning approach grounded in (art)making. In these works, a critical social consciousness was promoted among the participants by means of an enhanced social imagination provoked by the interplay between political content and artistic means. Essentially, the art works examined in this study serve as models of transdisciplinary art for learning and teaching social justice issues and civic engagement. Moreover, the results of this study encourage collaboration between artists and educators, as artists' approaches diversify pedagogy and, conversely, educators play a critical role in enhancing the learning experience of participants. [ABSTRACT FROM AUTHOR]

Interlayer excitons (IXs) at the interface of heterostructures (HSs) with a staggered band alignment are fascinating quantum quasi‐particles with light‐emitting and long‐lifetime characteristics. In this study, the energy band alignments (EBAs) of the HS of MAPbI3 perovskite thin sheets with CdSe‐ZnS core–shell quantum dot (QD) layers are modulated by using different diameters of the QDs. Far‐red IX emission is observed at 1.42 eV from the HS of MAPbI3/CdSe‐ZnS‐QD (λem = 645 nm) with type‐II EBA owing to charge transfer. The lifetime of the far‐red IXs is estimated to be 5.68 µs, which is considerably longer than that (0.715 ns) of the intralayer excitons from CdSe‐ZnS‐QD. With increasing incident excitation power, the PL peak and its intensity of IXs are blue‐shifted and linearly increased, respectively, indicating a strong dipole alignment of far‐red IXs at the heterojunction. Back focal plane imaging suggests that the directions of dipole moments of the IXs are relatively out‐of‐plane compared to those of the intralayer excitons (MAPbI3 and CdSe‐ZnS‐QD). Notably, the abnormal behavior of the optical characteristics is observed near the phase transition temperature (90 K) of MAPbI3. MAPbI3/CdSe‐ZnS‐QD HS photodetectors show the increase in photocurrent and detectivity compared to MAPbI3 at IX excitation. [ABSTRACT FROM AUTHOR]

Artmaking, when used as a form of pedagogy and approached in a socially‐conscious manner, has the potential to promote agency and create a democratic learning environment for students. This study examines one such project, "The Council," created by artist Adelita Husni‐Bey in collaboration with former Teen Program attendees of the Museum of Modern Art. The Council is a collection of large‐scale photographs created from a series of workshops in which 13 young adults imagined themselves reshaping the museum's societal role after a major global crisis. The final photographs were exhibited in the museum's main galleries, representing a participatory, socially‐engaged artwork produced from a pedagogical process. While there is a prevalence of these types of pedagogy‐based artworks, contemporary art literature tends to focus mainly on the artist, disregarding the participants. Therefore, from the stance of an art educator, I examined the learning experiences of the participants as well as the pedagogical framework of the artist. The reviews from the participants were overwhelmingly positive, with many noting a significant increase in their confidence and a greater sense of agency. A liberating experience of collaboration was also stated as a common experience. These outcomes were attributed to the artist's innovative use of multimodal learning and effective facilitation grounded in Francesc Ferrer's philosophy of anarcho‐collectivism and integral education, as well as critical pedagogy. This study suggests that creative methodologies can significantly enhance intrinsically motivated learning and emphasises the importance of nurturing the next generation as they envision a more equitable and just society. [ABSTRACT FROM AUTHOR]

A 12‐year‐old castrated male Shih‐Tzu dog was referred for uncontrolled glaucoma and uveitis with highly pigmented sclera, in both eyes (OU). On ophthalmic examination, the menace response, dazzle reflex and pupillary light reflex were negative OU. The intraocular pressure was 27 mmHg in the right eye (OD) and 70 mmHg in the left eye (OS) despite the administration of antiglaucoma eyedrops. Ultrasound biomicroscopy revealed a closed ciliary cleft OU. Ocular ultrasonography revealed hyperechoic materials in the vitreous OU and retinal detachment OS. When presented for recheck, an extensive malacic corneal ulcer was observed OS. To relieve pain in the blind eyes, enucleation OS and pharmacologic ciliary body ablation (CBA) OD were performed. Histologically, ocular melanosis, which is an inherited disease in the Cairn Terrier breed, was identified in the enucleated eye. The uvea was heavily pigmented. The iris and ciliary body were mildly distorted by a single population of large, round, nonneoplastic cells with pigmented cytoplasm. There was no evidence of an intraocular mass or metastasis before and after intravitreal CBA. This is the first report of bilateral ocular melanosis in a Shih‐Tzu dog. Ocular melanosis is a possible differential diagnosis for globe presenting scleral pigmentation with glaucoma in even non‐Cairn Terrier breeds and pharmacologic CBA could be considered as a treatment for ocular melanosis with end‐stage glaucoma. [ABSTRACT FROM AUTHOR]

Rehabilitation using digital healthcare (DHC) has the potential to enhance the effectiveness of treatment for musculoskeletal disorders (MSDs) and associated pain by improving patient outcomes, while being cost-effective, safe, and measurable. This systematic review and meta-analysis aimed to evaluate the effectiveness of musculoskeletal rehabilitation using DHC. We searched PubMed, Ovid-Embase, Cochrane Library, and PEDro Physiotherapy Evidence Database from inception to October 28, 2022 for controlled clinical trials comparing DHC to conventional rehabilitation. We used a random-effects model for the meta-analysis, pooling the effects of DHC on pain and quality of life (QoL) by calculating standardized mean differences (SMDs) with 95% confidence intervals (CIs) between DHC rehabilitation and conventional rehabilitation (control). Fifty-four studies with 6240 participants met the inclusion criteria. The sample size ranged from 26 to 461, and the average age of the participants ranged from 21.9 to 71.8 years. The majority of the included studies focused on knee or hip joint MSD (n = 23), and the most frequently utilized DHC interventions were mobile applications (n = 26) and virtual or augmented reality (n = 16). Our meta-analysis of pain (n = 45) revealed that pain reduction was greater in DHC rehabilitation than in conventional rehabilitation (SMD: − 0.55, 95% CI: − 0.74, − 0.36), indicating that rehabilitation using DHC has the potential to ameliorate MSD pain. Furthermore, DHC significantly improved health-related QoL and disease-specific QoL (SMD: 0.66, 95% CI: 0.29, 1.03; SMD: − 0.44, 95% CI: − 0.87, − 0.01) compared to conventional rehabilitation. Our findings suggest that DHC offers a practical and flexible rehabilitation alternative for both patients with MSD and healthcare professionals. Nevertheless, further researches are needed to elucidate the underlying mechanisms by which DHC affects patient-reported outcomes, which may vary depending on the type and design of the DHC intervention. [ABSTRACT FROM AUTHOR]

Traumatic spinal cord injury (SCI) causes secondary damage in injured and adjacent regions due to temporal deprivation of oxygen and energy supply. Peroxisome proliferator‐activated receptor γ (PPARγ) is known to regulate cell survival mechanisms such as hypoxia, oxidative stress, inflammation and energy homeostasis in various tissues. Thus, PPARγ has the potential to show neuroprotective properties. However, the role of endogenous spinal PPARγ in SCI is not well established. In this study, under isoflurane inhalation, a 10‐g rod was freely dropped onto the exposed spinal cord after T10 laminectomy using a New York University impactor in male Sprague–Dawley rats. Cellular localization of spinal PPARγ, locomotor function and mRNA levels of various genes including NFκB‐targeted pro‐inflammatory mediators after intrathecal administration of PPARγ antagonists, agonists or vehicles in SCI rats were then analysed. In both sham and SCI rats, spinal PPARγ was presented in neurons but not in microglia or astrocytes. Inhibition of PPARγ induced IκB activation and increased mRNA levels of pro‐inflammatory mediators. It also suppressed recovery of locomotor function with myelin‐related gene expression in SCI rats. However, a PPARγ agonist showed no beneficial effects on the locomotor performances of SCI rats, although it further increased the protein expression of PPARγ. In conclusion, endogenous PPARγ has a role in anti‐inflammation after SCI. Inhibition of PPARγ might have a negative influence on motor function recovery through accelerated neuroinflammation. Nonetheless, exogenous PPARγ activation does not appear to effectively help with functional improvement after SCI. [ABSTRACT FROM AUTHOR]

We report that a tetra-armed cyclen (L) with phenylethynylmethyl (Ph–C≡C–CH2–) groups as side-arms forms L·CH3CN (L·AN) inclusion crystals from acetonitrile–organic mixed solvents with high selectivity and acts as if it is an acetonitrile selective molecular sponge. [ABSTRACT FROM AUTHOR]

Acetamidinium bromoplumbate, denoted (ACA)PbBr3, was synthesized from acetamidinium bromide and lead bromide in liquid medium under ambient atmosphere. The large radius of the acetamidinium group of (ACA)PbBr3 accommodates the 4H BaRuO3 structure, which has a hexagonal array of infinite face‐shared and corner‐shared [PbBr6]4− units. (ACA)PbBr3 was characterized by single X‐ray diffraction, chemical analyses, thermal analyses, 1H NMR, and FT‐IR spectroscopy. [ABSTRACT FROM AUTHOR]