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Enhanced Point-of-Care SARS-CoV-2 Detection: Integrating RT-LAMP with Microscanning.
- Source :
- Biosensors (2079-6374); Jul2024, Vol. 14 Issue 7, p348, 15p
- Publication Year :
- 2024
-
Abstract
- The COVID-19 pandemic has highlighted the urgent need for rapid and accurate diagnostic methods for various infectious diseases, including SARS-CoV-2. Traditional RT-PCR methods, while highly sensitive and specific, require complex equipment and skilled personnel. In response, we developed an integrated RT-LAMP-MS assay, which combines rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) with microscanning (MS) technology for detecting SARS-CoV-2. The assay uses magnesium pyrophosphate formed during LAMP amplification as a visual marker, allowing direct observation via microscopy without the need for additional chemical indicators or probes. For the SARS-CoV-2/IC RT-LAMP-MS assay, the sample-LAMP reagent mixture was added to a microchip with SARS-CoV-2 primers and internal controls, then incubated at 62 °C for 30 min in a heat block, followed by amplification analysis using a microscanner. In clinical tests, the RT-LAMP-MS assay showed 99% sensitivity and 100% specificity, which is identical to the RT-LAMP results and comparable to the commercial Allplex<superscript>TM</superscript> SARS-CoV-2 assay results. Additionally, the limit of detection (LOD) was determined to be 10<superscript>−1</superscript> PFU mL<superscript>−1</superscript> (dynamic range: 10<superscript>3</superscript>~10<superscript>−1</superscript> PFU mL<superscript>−1</superscript>). The assay delivers results in 30 min, uses low-cost equipment, and demonstrates 100% reproducibility in repeated tests, making it suitable for point-of-care use in resource-limited settings. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20796374
- Volume :
- 14
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- Biosensors (2079-6374)
- Publication Type :
- Academic Journal
- Accession number :
- 178692916
- Full Text :
- https://doi.org/10.3390/bios14070348