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Objective: Unusual clinical course Background: Priapism is a persistent penile erection that lasts longer than 4 hours. The most common causes of priapism are ischemia and the effects of drugs, including PDE-5 inhibitors, such as sildenafil and anticoagulants. Here, we describe the presentation and management of a 50-year-old man with priapism following coronary artery angiography for the investigation of ischemic heart disease (IHD). Case Report: A 50-year-old man was admitted to the Department of Cardiovascular Disease with the chief concern of intermittent precordial pain and discomfort for 1 year. After performing regular medical tests in the hospital, we confirmed that there was a need to identify the cause of the chest pain. Thus, after obtaining informed consent, we performed coronary angiography and diagnosed the patient with coronary artery disease based on the results of coronary angiography. However, half an hour after coronary angiography, he developed priapism. By performing ultrasound of the penis, we found that there was a thrombus in the penile vein. By combining blood aspiration from the corpus cavernosum of the penis with injection of hydroxylamine into the corpus cavernosum, we achieved a good treatment effect. Conclusions: Priapism is becoming more common in middle-aged men. It should be treated as a medical emergency according to current management guidelines, and the underlying cause should be identified to prevent recurrence. [ABSTRACT FROM AUTHOR]

Introduction: Weeds are significant factors that detrimentally affect crop health and hinder optimal herbage yield. Rhizosphere microorganisms play crucial roles in plant growth, development, and nutrient uptake. Therefore, research focusing on weed control through the lens of microorganisms has emerged as a prominent area of study. The oil-producing fungus Mortierella, which is known for its numerous agricultural benefits, has garnered significant attention in recent years. Methods: In this study, we conducted inoculation experiments in a controlled artificial culture climate chamber to investigate the effects of differential hormones and differentially expressed genes in the stems and leaves of Digitaria sanguinalis using Liquid Chromatography Tandem Mass Spectrometry and RNA-seq techniques, respectively. Additionally, Pearson's correlation analysis was used to establish correlations between differential hormones and growth indicators of Digitaria sanguinalis. Results and discussion: The results demonstrated that inoculation with Mortierella sp. MXBP304 effectively suppressed aboveground biomass and plant height in Digitaria sanguinalis. Furthermore, there was significant upregulation and downregulation in the expression of genes involved in the synthesis and metabolism of phenylalanine and L-phenylalanine. Conversely, the expression of genes related to tryptophan, L-tryptophan, and indole was significantly downregulated. The addition of Mortierella sp. MXBP304 can influence the gene expression associated with phenylalanine and tryptophan synthesis and metabolism during Digitaria sanguinalis growth, subsequently reducing the relative contents of phenylalanine and tryptophan, thereby directly inhibiting Digitaria sanguinalis growth. [ABSTRACT FROM AUTHOR]

Context: The relationship between vitamin D and thyroid profiles lacks consensus despite extensive investigations. Whether vitamin D levels correlate with thyroid hormone sensitivity remains largely unexplored. Objective: To explore the relationship between vitamin D levels and thyroid hormone sensitivity among euthyroid individuals. Methods: This study involved 6452 euthyroid participants. Clinical parameters, including TSH, free thyroxine, 25-hydroxyvitamin D [25(OH)D], and other relevant indicators were extracted from the National Health and Nutrition Examination Survey 2007-2012. To quantify thyroid hormone sensitivity, we calculated the Thyroid Feedback Quantile-based Index (TFQI), the TSH index (TSHI), and the thyrotropin thyroxine resistance index (TT4RI). Results: Subjects with impaired thyroid hormone sensitivity have decreased 25(OH)D levels (TFQI, TT4RI: P < 0.05; TSHI: P = .05574) following adjustment of confounding variables. Age-specific analysis found negative correlations between thyroid hormone sensitivity indices and 25(OH)D within the 20 to 60 years subgroup, turning positive in the 60 to 80 years subgroup. In females, thyroid hormone sensitivity indices and vitamin D levels were negatively linked, while in males, vitamin D’s relationships with TFQI, TT4RI, and TSHI shifted from negative to positive when 25(OH) D levels exceeded 63.5 nmol/L, 56.7 nmol/L, and 56.7 nmol/L, respectively. Stratification by race revealed U-shaped curvilinear patterns resembling those found in the males. In body mass index (BMI) subanalysis, vitamin D had differing associations with thyroid hormone sensitivity indices: negative in the <25 kg/m² and ≥30 kg/m² subgroups and U-shaped in the 25-30 kg/m² subgroup. Conclusion: Impaired thyroid hormone sensitivity correlates with decreased vitamin D levels among euthyroid subjects, with associations varying by age, sex, race, and BMI. [ABSTRACT FROM AUTHOR]

The stability of the subsea oil and gas production system is heavily influenced by slug flow. One successful method of managing slug flow is to use top valve control based on subsea pipeline pressure. However, the complexity of production makes it difficult to measure the pressure of subsea pipelines, and measured values are not always accessible in real-time. The research introduces a technique for integrating Unscented Kalman Filter (UKF) and Wavelet Neural Network (WNN) to estimate the state of subsea pipeline pressure using historical data and a state model. The proposed method treats multiphase flow transport as a nonlinear model, with a dynamic WNN serving as the state observer. To achieve realtime state estimation, the WNN is included into the UKF algorithm to create a WNN-based UKF state equation. Integrate WNN and UKF in a novel way to predict system state accurately. The simulated results show that the approach can efficiently predict the inlet pressure and manage the slug flow in real-time using the riser's top pressure, outlet flow and valve opening. This method of estimate can significantly increase the control effect. [ABSTRACT FROM AUTHOR]

Background: Chimeric antigen receptor (CAR) T-cell therapy has demonstrated high initial complete remission (CR) rates in B-cell acute lymphoblastic leukemia (B-ALL) patients, including those who relapsed after transplant. However, the duration of remission requires improvements. Whether bridging to a second allogeneic hematopoietic stem cell transplant (allo-HSCT) after CAR-T therapy can improve long-term survival remains controversial. We retrospectively analyzed long-term follow-up data of B-ALL patients who relapsed posttransplant and received CAR-T therapy followed by consolidation second allo-HSCT to investigate whether such a treatment sequence could improve longterm survival. Methods: A single-center, retrospective study was performed between October 2017 and March 2022, involving 95 patients who received a consolidation second transplant after achieving CR from CAR-T therapy. Results: Themedian age of patients was 22.8 years (range: 3.3-52.8) at the second transplant. After the first transplant, 71 patients (74.7%) experienced bone marrow relapse, 16 patients (16.8%) had extramedullary relapse, 5 patients (5.3%) had both bone marrow and extramedullary relapse and 3/95 patients (3.2%) had positive minimal residual disease (MRD) only. Patients received autologous (n=57, 60.0%) or allogeneic (n=28, 29.5%) CAR-T cells, while 10 patients (10.5%) were unknown. All patients achieved CR after CAR-T therapy. Before second HSCT, 86 patients (90.5%) were MRD-negative, and 9 (9.5%) were MRD-positive. All second transplant donors were different from the first transplant donors. The median follow-up time was 623 days (range: 33-1901) after the second HSCT. The 3-year overall survival (OS) and leukemia-free survival (LFS) were 55.3% (95%CI, 44.3-66.1%) and 49.8% (95%CI, 38.7-60.9%), respectively. The 3-year relapse incidence (RI) and non-relapse mortality (NRM) were 10.5% (95%CI, 5.6-19.6%) and 43.6% (95%CI, 33.9-56.2%), respectively. In multivariate analysis, the interval fromCAR-Tto second HSCT =90 days was associated with superior LFS(HR, 4.10, 95%CI, 1.64-10.24; p=0.003) and OS(HR, 2.67, 95%CI, 1.24-5.74, p=0.012), as well as reduced NRM (HR, 2.45, 95%CI, 1.14-5.24, p=0.021). Conclusions: Our study indicated that CAR-T therapy followed by consolidation second transplant could significantly improve long-term survival in B-ALL patients who relapsed post-transplant. The second transplant should be considered in suitable patients and is recommended to be performed within 90 days after CAR-T treatment. [ABSTRACT FROM AUTHOR]

Diabetes mellitus is an established risk factor for periodontal disease that can aggravate the severity of periodontal inflammation and accelerate periodontal destruction. The chronic high glucose condition is a hallmark of diabetes-related pathogenesis, and has been demonstrated to impair the osteogenic differentiation of periodontal ligament stem cells (PDLSCs), leading to delayed recovery of periodontal defects in diabetic patients. Reactive oxygen species (ROS) are small molecules that can influence cell fate determination and the direction of cell differentiation. Although excessive accumulation of ROS has been found to be associated with high glucose-induced cell damage, the underlying mechanisms remain unclear. Nicotinamide adenine dinucleotide phosphate (NADPH) is an important electron donor and functions as a critical ROS scavenger in antioxidant systems. It has been identified as a key mediator of various biological processes, including energy metabolism and cell differentiation. However, whether NADPH is involved in the dysregulation of ROS and further compromise of PDLSC osteogenic differentiation under high glucose conditions is still not known. In the present study, we found that PDLSCs incubated under high glucose conditions showed impaired osteogenic differentiation, excessive ROS accumulation and increased NADPH production. Furthermore, after inhibiting the synthesis of NADPH, the osteogenic differentiation of PDLSCs was significantly enhanced, accompanied by reduced cellular ROS accumulation. Our findings demonstrated the crucial role of NADPH in regulating cellular osteogenic differentiation under high glucose conditions and suggested a new target for rescuing high glucose-induced cell dysfunction and promoting tissue regeneration in the future. [ABSTRACT FROM AUTHOR]

Background: For patients of primary brainstem hemorrhage (PBH), it is crucial to find a method that can quickly and accurately predict the correlation between status of consciousness and PBH. Objective: To analyze the value of computer tomography (CT) signs in combination with artificial intelligence (AI) technique in predicting the correlation between status of consciousness and PBH. Methods: A total of 120 patients with PBH were enrolled from August 2011 to March 2021 according to the criteria. Patients were divided into three groups [consciousness, minimally conscious state (MCS) and coma] based on the status of consciousness. Then, first, Mann-Whitney U test and Spearman rank correlation test were used on the factors: gender, age, stages of intracerebral hemorrhage, CT signs with AI or radiology physicians, hemorrhage involving the midbrain or ventricular system. We collected hemorrhage volumes and mean CT values with AI. Second, those significant factors were screened out by the Mann-Whitney U test and those highly or moderately correlated by Spearman's rank correlation test, and a further ordinal multinomial logistic regression analysis was performed to find independent predictors of the status of consciousness. At last, receiver operating characteristic (ROC) curves were drawn to calculate the hemorrhage volume for predictively assessing the status of consciousness. Results: Preliminary meaningful variables include hemorrhage involving the midbrain or ventricular system, hemorrhage volume, grade of hematoma shape and density, and CT value from Mann-Whitney U test and Spearman rank correlation test. It is further shown by ordinal multinomial logistic regression analysis that hemorrhage volume and hemorrhage involving the ventricular system are two major predictors of the status of consciousness. It showed from ROC that the hemorrhage volumes of <3.040 mL, 3.040 ~ 6.225 mL and >6.225 mL correspond to consciousness, MCS or coma, respectively. If the hemorrhage volume is the same, hemorrhage involving the ventricular system should be correlated with more severe disorders of consciousness (DOC). Conclusion: CT signs combined with AI can predict the correlation between status of consciousness and PBH. Hemorrhage volume and hemorrhage involving the ventricular system are two independent factors, with hemorrhage volume in particular reaching quantitative predictions. [ABSTRACT FROM AUTHOR]

Objective: Dementia with Lewy bodies (DLB) and Parkinson's disease dementia (PDD) are collectively known as Lewy body dementia (LBD). Considering the heterogeneous nature of LBD and the different constellations of symptoms with which patients can present, the exact molecular mechanism underlying the differences between these two isoforms is still unknown. Therefore, this study aimed to explore the biomarkers and potential mechanisms that distinguish between PDD and DLB. Methods: The mRNA expression profile dataset of GSE150696 was acquired from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) between 12 DLB and 12 PDD were identified from Brodmann area 9 of human postmortembrains using GEO2R. A series of bioinformaticsmethods were applied to identify the potential signaling pathways involved, and a protein-protein interaction (PPI) network was constructed. Weighted gene co-expression network analysis (WGCNA) was used to further investigate the relationship between gene co-expression and different LBD subtypes. Hub genes that are strongly associated with PDD and DLB were obtained from the intersection of DEGs and selected modules by WGCNA. Results: A total of 1,864 DEGs between PDD and DLB were filtered by the online analysis tool GEO2R. We found that the most significant GO- and KEGG-enriched terms are involved in the establishment of the vesicle localization and pathways of neurodegeneration-multiple diseases. Glycerolipid metabolism and viral myocarditis were enriched in the PDD group. A B-cell receptor signaling pathway and one carbon pool by folate correlated with DLB in the results obtained from the GSEA. We found several clusters of co-expressed genes which we designated by colors in our WGCNA analysis. Furthermore, we identified seven upregulated genes, namely, SNAP25,GRIN2A,GABRG2,GABRA1,GRIA1, SLC17A6, and SYN1, which are significantly correlated with PDD. Conclusion: The seven hub genes and the signaling pathways we identified may be involved in the heterogeneous pathogenesis of PDD and DLB. [ABSTRACT FROM AUTHOR]

Clinical observation indicates that exercise capacity, an important determinant of survival in patients with congenital heart disease (CHD), is most decreased in children with reduced pulmonary blood flow (RPF). However, the underlying mechanism remains unclear. Here, we obtained human RPF lung samples from children with tetralogy of Fallot as well as piglet and rat RPF lung samples from animals with pulmonary artery banding surgery. We observed impaired alveolarization and vascularization, the main characteristics of pulmonary dysplasia, in the lungs of RPF infants, piglets, and rats. RPF caused smaller lungs, cyanosis, and body weight loss in neonatal rats and reduced the number of alveolar type 2 cells. RNA sequencing demonstrated that RPF induced the downregulation of metabolism and migration, a key biological process of late alveolar development, and the upregulation of immune response, which was confirmed by flow cytometry and cytokine detection. In addition, the immunosuppressant cyclosporine A rescued pulmonary dysplasia and increased the expression of the Wnt signaling pathway, which is the driver of postnatal lung development. We concluded that RPF results in pulmonary dysplasia, which may account for the reduced exercise capacity of patients with CHD with RPF. The underlying mechanism is associated with immune response activation, and immunosuppressants have a therapeutic effect in CHD-associated pulmonary dysplasia. [ABSTRACT FROM AUTHOR]

Background: The spleen is the largest peripheral lymphoid organ in the body. Studies have implicated the spleen in the development of cancer. However, it is unknown whether splenic volume (SV) is associated with the clinical outcome of gastric cancer. Methods: Data of gastric cancer patients treated with surgical resection were retrospectively analyzed. Patients were divided into three groups: underweight, normal-weight and overweight. Overall survival was compared in patients with high and low splenic volume. The correlation between splenic volume and peripheral immune cells were analyzed. Results: Of 541 patients, 71.2% were male and the median age was 60. Underweight, normal-weight and overweight patients accounted for 5.4%, 62.3% and 32.3%, respectively. High splenic volume was associated with unfavorable prognosis across the three groups. In addition, the increase of splenic volume during neoadjuvant chemotherapy was not associated with prognosis. The baseline splenic volume was negatively correlated with lymphocytes (r=-0.21, p<0.001) and positively correlated with NLR (neutrophil-to-lymphocyte ratio) (r=0.24, p<0.001). In a group of patients (n=56), splenic volume was found to have negative correlation with CD4+T cells (r=-0.27, p=0.041) and NK cells (r=- 0.30, p=0.025). Conclusions: The presence of high splenic volume is a biomarker of unfavorable prognosis and reduced circulating lymphocytes in gastric cancer. [ABSTRACT FROM AUTHOR]

Background: We aimed to compare the efficacy of chimeric antigen receptor T (CAR-T) cell therapy with that of autologous stem cell transplantation (auto-HSCT) in relapsed/refractory diffuse large B cell lymphoma (R/R DLBCL). Research design and methods: We searched eligible publications up to January 31st, 2022, in PubMed, Cochrane Library, Springer, and Scopus. A total of 16 publications with 3484 patients were independently evaluated and analyzed using STATA SE software. Results: Patients who underwent CAR-T cell therapy showed a better overall response rate (ORR) and partial response (PR) than those treated with auto-HSCT (CAR-T vs. auto-HSCT, ORR: 80% vs. 73%, HR:0.90,95%CI:0.76-1.07,P = 0.001; PR: 20% vs. 14%, HR:0.65,95%CI:0.62-0.68,P = 0.034). No significant difference was observed in 6-month overall survival (OS) (CAR-T vs. auto-HSCT, six-month OS: 81% vs. 84%, HR:1.23,95%CI:0.63-2.38, P = 0.299), while auto-HSCT showed a favorable 1 and 2-year OS (CAR-T vs. auto-HSCT, one-year OS: 64% vs. 73%, HR:2.42,95%CI:2.27-2.79, P < 0.001; two-year OS: 54% vs. 68%, HR:1.81,95% CI:1.78-1.97, P < 0.001). Auto-HSCT also had advantages in progression-free survival (PFS) (CAR-T vs. auto-HSCT, six-month PFS: 53% vs. 76%, HR:2.81,95% CI:2.53-3.11,P < 0.001; one-year PFS: 46% vs. 61%, HR:1.84,95%CI:1.72-1.97,P < 0.001; two-year PFS: 42% vs. 54%, HR:1.62,95%CI:1.53-1.71, P < 0.001). Subgroup analysis by age, prior lines of therapy, and ECOG scores was performed to compare the efficacy of both treatment modalities. Conclusion: Although CAR-T cell therapy showed a beneficial ORR, auto-HSCT exhibited a better long-term treatment superiority in R/R DLBCL patients. Survival outcomes were consistent across different subgroups. [ABSTRACT FROM AUTHOR]

Livestock grazing is a dominant practice in alpine grasslands and plays a crucial role in the ecosystem service of the Qinghai-Tibetan Plateau. The effects of grazing on alpine grasslands highly depends on grazing intensity. Up to now, we still lack comprehensive understanding of the general responses of alpine grasslands to different grazing intensities over broad geographic scales across the Qinghai-Tibetan Plateau. Here, we conducted a meta-analysis to explore the responses of plant characteristics and soil properties to grazing intensity in alpine grasslands of the Qinghai-Tibetan Plateau based on 52 peer-reviewed literatures. The results showed that grazing did not change the belowground biomass, while significantly increased the ratio of root to shoot (P< 0.05). Light grazing exhibited no significant effects on the plant richness, Shannon-Wiener diversity, soil water content, soil bulk density, nutrients, microbial biomass carbon, and microbial biomass nitrogen (P > 0.05). Moderate grazing significantly increased the plant richness and Shannon-Wiener diversity, while significantly decreased the soil organic carbon and total nitrogen (P< 0.05). Heavy grazing significantly decreased the plant richness, Shannon-Wiener diversity, water content, soil organic carbon, total nitrogen, microbial biomass carbon, and microbial biomass nitrogen, and significantly increased the soil bulk density (P< 0.05). These findings suggest that overgrazing is closely associated with grassland degradation, and moderate grazing is a sustainable practice to provide animal production and simultaneously maintain ecological functions for alpine grasslands on the Qinghai-Tibetan Plateau. [ABSTRACT FROM AUTHOR]

Introduction: We aimed to evaluate prognostic factors of a second allogeneic stem cell transplantation (allo-HSCT2) among hematological malignancy patients who have relapsed after the first allo-HSCT(allo-HSCT1). Methods: We retrospectively analyzed 199 hematological malignancy patients who received allo-HSCT2 as a salvage treatment post allo-HSCT1 relapse between November 2012 and October 2021. Results: The median age at allo-HSCT2 was 23 (range: 3-60) years. The median time to relapse after HSCT1 was 9 (range: 1-72) months. Prior to allo-HSCT2, patients had the following hematopoietic cell transplantation-comorbidity indexes (HCT-CI): 127 with a score of 0, 52 with a score of 1, and 20 with a score of 2 or greater. Fifty percent of patients received chimeric antigen receptor (CAR) T-cell therapy following HSCT1 relapse. Disease status was minimal residual disease (MRD)-negative complete remission (CR) among 119 patients, MRD-positive CR among 37 patients and non-remission (NR) for 43 patients prior to allo-HSCT2. Allo-HSCT2 was performed from a new donor in 194 patients (97.4%) and 134 patients (67.3%) received a graft with a new mismatched haplotype. The median follow-up time was 24 months (range: 6- 98 months), and the 2-year OS and LFS were 43.8% ± 4.0% and 42.1% ± 4.1%, respectively. The 2-year cumulative incidence of relapse (CIR) and non-relapse mortality (NRM) was 30.0%±4.8% and 38.5%±3.8%, respectively. Cox regression multivariate analysis showed that disease statusof MRD-negative CR, HCT-CI score of 0 prior to allo-HSCT2, and new mismatched haplotype donor were predictive factors of improved OS and LFS compared to patients without these characteristics. Based on these three favorable factors, we developed a predictive scoring system for patients who received allo-HSCT2. Patients with a prognostic score of 3 who had the three factors showed a superior 2-year OS of 63.3% ± 6.7% and LFS of 63.3% ± 6.7% and a lower CIR of 5.5% ± 3.1% than patients with a prognostic score of 0. Allo-HSCT2 is feasible and patients with good prognostic features prior to allo-HSCT2 --disease status of CR/MRD- and HCT-CI score of 0 as well as a second donor with a new mismatched haplotype could have the maximal benefit from the second allo-HSCT. Conclusions: Allo-HSCT2 is feasible and patients with good prognostic features prior to allo-HSCT2 --disease status of CR/MRD- and HCT-CI score of 0 as well as a second donor with a new mismatched haplotype could have the maximal benefit from the second allo-HSCT. [ABSTRACT FROM AUTHOR]

Background: Good adherence to post-operative exercise is crucial for long-term exercise intervention programs aimed at improving compliance, motivation for functional exercise, and quality of life among breast cancer patients. This study aims to evaluate the application effect of quality control circles on postoperative functional exercise compliance in breast neoplasm patients. Methods: A comprehensive search was conducted in CNKI, Wanfang, VIP, CBM, PubMed, Cochrane Library, Web of Science and CINAHL databases from their establishment to May 06, 2023. Manual search and backtracking of relevant literature were also conducted. The search terms included a combination of subject terms and free words. Meta-analysis was performed using RevMan 5.4 software on eligible literature. Results: Initially, 517 papers were retrieved, and after removing duplicates 238 papers using NoteExpress software, 279 titles and abstracts were reviewed. Subsequently, 225 non-compliant papers were excluded, leaving 54 full texts to be read. After excluding 38 non-compliant papers, 16 eligible papers were included for Meta-analysis. These papers comprised a total of 1,342 patients (679 in the test group and 663 in the control group). The heterogeneity test using a fixed effects model showed P = 0.22 and I2 = 21%. The Meta-analysis results demonstrated that the implementation of quality control circles alongside usual care significantly improved compliance with postoperative functional exercise in breast cancer patients (odds ratio = 6.68, 95% confidence interval (4.97, 8.98), P < 0.00001). Conclusion: Quality control circles activities can effectively enhance compliance with post-operative functional exercise among breast cancer patients, aiding in the recovery of affected limb function, improvement of self-care ability, and early reintegration into society and normal life, thereby enhancing quality of life. It is important to continually accumulate experience in applying relevant quality control circles activities in clinical work and promptly enhance the level of care accordingly, as the functional exercise of breast cancer patients after surgery involves a long-term and gradual improvement process. [ABSTRACT FROM AUTHOR]

Objective: To investigate the therapeutic effect of Bai Hu Ren Shen Soup (BHRSS) on type 2 diabetes mellitus (T2DM) rats and explore whether its therapeutic effect is achieved by promoting autophagy in the pancreas tissue of T2DM rats. Methods: Thirty male standard deviation rats were equally divided into normal control group, T2DM group and BHRSS group. Firstly, the therapeutic effect of BHRSS on T2DM was evaluated by biochemical indices in serum and pathological changes in the pancreas tissue of rats in each group after modeling and medication. Then, the levels of microtubule-associated protein 1 light chain 3 (LC3) protein and p62 protein in the pancreas tissue of rats in each group after BHRSS intervention were detected by Western blot. Results: BHRSS intervention significantly reduced the levels of blood sugar, liver and kidney function, and other related biochemical indices in the serum of T2DM rats, and effectively improved the pathological changes in the pancreas tissue of T2DM rats. In addition, Western blot results showed that the expression levels of autophagy-related protein LC3 protein in the pancreas tissue of rats were increased, and the expression level of p62 protein was decreased after BHRSS intervention. Conclusion: BHRSS has a clear therapeutic effect on T2DM model rats, which may be related to that increase of autophagy protein LC3 and the decrease of p62 protein level in the pancreas tissue. [ABSTRACT FROM AUTHOR]

Phosphite, a reduced form of orthophosphate, is characterized by high solubility, and transportation efficiency and can be used as potential phosphorus fertilizer, plant biostimulant and supplemental fertilizer in agriculture. However, the effects of phosphite fertilizer on soil properties and microorganisms are poorly understood. This study evaluated the effects of phosphate and phosphite fertilizers on the different forms of phosphorus, alkaline phosphatase (ALP) activity, and phoD-harboring bacterial community in the alfalfa (Medicago sativa) field. The study used four concentrations (30, 60, 90, and 120 mg P2O5 kg−1 soil) of phosphate (KH2PO4) and phosphite (KH2PO3) fertilizers for the alfalfa field treatment. The results showed that both phosphite and phosphate fertilizers increased the total phosphorus (TP) and available phosphorus (AP) contents in the soil. The phosphorus content of the phosphite-treated soil was lower than that of the phosphate-treated one. TP, inorganic phosphate (Pi), and AP negatively regulated ALP activity, which decreased with increasing phosphate and phosphite fertilizers concentrations. Furthermore, high-throughput sequencing analysis identified 6 phyla and 29 families, which were classified from the altered operational taxonomic units (OTUs) of the soil samples. The redundancy analysis (RDA) revealed that pH, TP, AP and Pi were significantly related to the phoD-harboring bacterial community constructure. The different fertilizer treatments altered the key families, contributing to soil ALP activities. Frankiaceae, Sphingomonadaceae, and Rhizobiaceae positively correlated with ALP activity in phosphite-treated soil. Moreover, the structural equation model (SEM) revealed that ALP activity was affected by the phoD-harboring bacterial community through altered organic phosphorus (Po), AP, total nitrogen (TN), soil organic carbon (SOC), and pH levels under phosphate fertilizer treatment. However, the effect was achieved through positive regulation of pH and AP under phosphite fertilizer. Thus, the changes in soil properties and phoD-harboring bacteria in response to phosphate and phosphite treatments differed in the alfalfa field. This study is the first to report the effects of phosphite on the soil properties of an alfalfa field and provides a strong basis for phosphite utilization in the future. [ABSTRACT FROM AUTHOR]

Seashore paspalum is a halophytic, warm-season grass with wide applications. It is noted for its superior salt tolerance in saline environments; however, the nutritive value of seashore paspalum and the effect of salinity remains to be determined. Therefore, this study aimed to evaluate the relationship between agronomic traits and forage quality and identified the effects of short-term high-salt stress (1 week, 700mMNaCl) on the growth and forage nutritive value of 16 ecotypes of seashore paspalum. The salt and cold tolerances of the seashore paspalum ecotypes were assessed based on the survival rate following long-term high-salt stress (7 weeks, 700 mM NaCl) and exposure to natural low temperature stress. There were significant genetic (ecotypespecific) effects on plant height, leaf-stem ratio, and survival rate of seashore paspalum following salt or low temperature stress. Plant height was significantly negatively correlated with the leaf-stem ratio (r = -0.63, P<0.01), but the heights and leaf-stem ratios were not significantly correlated with the fresh weight (FW) and dry weight (DW) of the shoots. High salinity decreased the FW and DW of the shoots by 50.6% and 23.6%, respectively, on average. Seashore paspalum exhibited outstanding salt tolerance and forage quality at high salinity. The survival rate of the different ecotypes of seashore paspalum varied from 6.5% to 49.0% following treatment with 700 mM NaCl for 7 weeks. The crude protein (CP) content of the control and treatment groups (700 mM NaCl) was 17.4% and 19.3%, respectively, of the DW on average, and the CP content of most ecotypes was not significantly influenced by high salinity. The average ether extract (EE) content ranged from 4.6% to 4.4% of the DW under control and saline conditions, respectively, indicating that the influence was not significant. The neutral detergent fiber (NDF) and acid detergent fiber (ADF) contents of the control group were 57.4% and 29.8%, respectively, of the DW on average. Salt stress reduced the content of NDF and ADF to 50.2% and 25.9%, respectively, of the DW on average. Altogether, the results demonstrated that stress did not have any significant effects on the CP and EE content of most ecotypes, but reduced the NDF and ADF content and improved relative feed value (RFV). The results obtained herein support the notion that seashore paspalum is a good candidate for improving the forage potential of saline soils and can provide useful guidelines for livestock producers. [ABSTRACT FROM AUTHOR]

The deficiency of available phosphate significantly limits plant growth and development. This study sought to investigate how alfalfa (Medicago sativa), a high-yielding and high-quality forage widely cultivated worldwide, responds to phosphate deficiency stress by integrating transcriptional and post-transcriptional data. In this study, 6,041 differentially expressed genes (DEGs) were identified in alfalfa roots under phosphate deficiency conditions. Furthermore, psRNATarget, RNAhybrid, and TargetFinder were used to predict the target genes of 137 differentially expressed miRNAs (DEMs) in the root. In total, 3,912 DEGs were predicted as target genes. Pearson correlation analysis revealed 423 pairs of miRNA-mRNA regulatory relationships. MiRNA negatively regulates mRNA involved in regulatory pathways of phosphate deficiency responses in alfalfa. miR156e targeted squamosa promoter-binding-like protein 13A (SPL13), miR160c targeted auxin response factor 18 (ARF18), and miR2587a controlled glycolysis and citrate cycle via Phosphoenolpyruvate carboxykinase (ATP) (PCKA). Novel-miR27 regulated SPX domain-containing protein that controls phosphate transport in alfalfa root, novel-miR3-targeted sulfoquinovosyl transferase SQD2 controlled sulfolipid synthesis and glutathione S-transferase (GST; mediated by miR169j/k and novel-miR159) regulated glutathione metabolism. miR399l regulated auxin-responsive protein SAUR72 involved in IAA signal transduction, while abscisic acid receptor PYL4 (regulated by novel-miR205 and novel-miR83) participated in ABA signal transduction. Combined miRNA-mRNA enrichment analysis showed that most miRNAs regulate the phosphate starvation response of alfalfa by modulating target genes involved in carbohydrate metabolism, sulfolipid metabolism, glutathione metabolism, and hormone signal transduction. Therefore, this study provides new insights into the post-transcriptional regulation mechanism of phosphate deficiency responses and new perspectives on phosphate assimilation pathways in alfalfa and other legumes. [ABSTRACT FROM AUTHOR]

Background: Significant survival benefit of adjuvant imatinib therapy has been observed in gastrointestinal stromal tumor (GIST). However, the impact of neoadjuvant imatinib on prognosis of GIST remains unclear. This meta-analysis aimed to compare the prognostic impact between upfront surgery and neoadjuvant imatinib plus surgery on GIST. Methods: A comprehensive literature search was performed to identify eligible studies up to 30 Sep 2021, through PubMed, Embase, Web of Science, and Cochrane Library. Studies compared the impact of upfront surgery and neoadjuvant imatinib plus surgery on disease-free (DFS) or overall survival (OS) in patients with GIST were selected. Results: Seven eligible studies with 17,171 patients were included. The reduction rates of tumor size in rectal and mixed site GIST were 33% and 29.8%, respectively. Neoadjuvant imatinib was not significantly associated with DFS compared with no-neoadjuvant therapy in rectal GIST (HR: 0.71, 95% CI: 0.35-1.41). The OS of rectal GIST was significantly improved by neoadjuvant imatinib compared with no-neoadjuvant therapy (HR: 0.36, 95% CI: 0.17-0.75). Conclusion: Neoadjuvant imatinib therapy contributed to tumor shrinkage and R0 resection of rectal GIST. Neoadjuvant imatinib plus surgery significantly improved overall survival of rectal GIST in comparison with upfront surgery. [ABSTRACT FROM AUTHOR]

Sweet sorghum (Sorghum dochna) is a high-quality bio-energy crop that also serves as food for humans and animals. However, there is little information on the genomic characteristics of S. dochna. In this study, we presented a high-quality assembly of S. dochna with PacBio long reads, Illumina short reads, high-throughput chromosome capture technology (Hi-C) sequencing data, gene annotation, and a comparative genome analysis. The results showed that the genome of S. dochna was assembled to 777 Mb with a contig N50 of 553.47 kb and a scaffold N50 of 727.11 kb. In addition, the gene annotation predicted 37,971 genes and 39,937 transcripts in the genome of S. dochna. A Venn analysis revealed a set of 7,988 common gene annotations by integrating five databases. A Cafe software analysis showed that 191 gene families were significantly expanded, while 3,794 were significantly contracted in S. dochna. A GO enrichment analysis showed that the expanded gene families were primarily clustered in the metabolic process, DNA reconstruction, and DNA binding among others. The high-quality genome map constructed in this study provides a biological basis for the future analysis of the biological characteristics of S. dochna, which is crucial for its breeding. [ABSTRACT FROM AUTHOR]

Autotoxicity is a form of intraspecific allelopathy, in which a plant species inhibits the establishment or growth of the same species through the release of toxic chemical compounds into the environment. The phenomenon of autotoxicity in crops is best traced in alfalfa (Medicago sativa). A close relative of alfalfa, M. truncatula, has been developed into an excellent model species for leguminous plants. However, it is not known whether M. truncatula has autotoxicity. In this study, M. truncatula root exudates showed a negative impact on the growth of M. truncatula seedlings, indicating autotoxicity. Detailed analyses with plant extracts from M. truncatula and alfalfa revealed varying degrees of suppression effects in the two species. The extracts negatively affected seed germination potential, germination rate, radicle length, hypocotyl length, synthetic allelopathic effect index, plant height, root growth, fresh weight, dry weight, net photosynthetic rate, transpiration rate, and stomatal conductance in both M. truncatula and alfalfa. The results demonstrated that autotoxicity and allelopathic effects exist in M. truncatula. This opens up a new way to use M. truncatula as a model species to carry out in-depth studies of autotoxicity and allelopathy to elucidate biochemical pathways of allelochemicals and molecular networks controlling biosynthesis of the chemicals. [ABSTRACT FROM AUTHOR]

Autotoxicity is a form of intraspecific allelopathy, in which a plant species inhibits the establishment or growth of the same species through the release of toxic chemical compounds into the environment. The phenomenon of autotoxicity in crops is best traced in alfalfa (Medicago sativa). A close relative of alfalfa, M. truncatula, has been developed into an excellent model species for leguminous plants. However, it is not known whether M. truncatula has autotoxicity. In this study, M. truncatula root exudates showed a negative impact on the growth of M. truncatula seedlings, indicating autotoxicity. Detailed analyses with plant extracts from M. truncatula and alfalfa revealed varying degrees of suppression effects in the two species. The extracts negatively affected seed germination potential, germination rate, radicle length, hypocotyl length, synthetic allelopathic effect index, plant height, root growth, fresh weight, dry weight, net photosynthetic rate, transpiration rate, and stomatal conductance in both M. truncatula and alfalfa. The results demonstrated that autotoxicity and allelopathic effects exist in M. truncatula. This opens up a new way to use M. truncatula as a model species to carry out in-depth studies of autotoxicity and allelopathy to elucidate biochemical pathways of allelochemicals and molecular networks controlling biosynthesis of the chemicals. [ABSTRACT FROM AUTHOR]

In view of the high content of Cu2+ and Zn2+ in acid mine drainage (AMD), the adsorption properties of lignite for Cu2+ and Zn2+ were studied. The adsorption performance of lignite for Cu2+ and Zn2+ was revealed by combining with FT-IR, XPS and EDS. The results showed that the adsorption kinetic model of lignite for Cu2+ and Zn2+ conformed to the quasi-first-order kinetic model. The isothermal adsorption line fitting models of lignite for Cu2+ and Zn2+ were in accordance with the Langmuir model and the Freundlich model, respectively. The maximum equilibrium adsorption capacities of lignite for Cu and Zn were 67.84 mg/g and 55.5 mg/g. The adsorption process of Cu2+ and Zn2+ by lignite involved electrostatic, coordination and ion exchange. Under the condition of coexistence of two kinds of ion, the adsorption site of lignite had stronger binding ability to copper ions. [ABSTRACT FROM AUTHOR]

It have been reported that the transition metal complex of Schiff base ligands have the ability to inhibit urease. Herein, in this manuscript, two novel transition metal complexes [Cd2(L)2·(H2O)] (1), [Pb(L)·, [Pbe2) were synthesized based on chelating hydrazone ligand N'-(pyridin-2-ylmethylene)isonicotinohydrazide (L). These compounds were tested by single crystal X-ray diffraction and determined by structure and tested for urease resistance in vitro. The complex 2 exhibited better the activities of inhibiting than the positive control acetohydroxamic acid with an IC50 =2.5 μM, while the complex 1 showed weak activity, and complex 2 acted as an effective urease inhibitor. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of high-dose recombinant human growth hormone(rhGH) treatment on insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-3 (IGFBP-3) levels in patients with idiopathic dwarfism. Methods: This study retrospectively investigated records of idiopathic dwarfism patients treated at Anhui Children's Hospital or the Affiliated Hospital of Xuzhou Medical University between May 2019 and October 2020. The study identified 76 patients, which were divided based on rhGH treatment dosage into high- and low-dose groups. Results: The high-dose group showed a total efficacy of 95.3%, which was significantly higher than the 79.41% observed in the low-dose group (P<0.05). Moreover, height, weight, IGF-1 levels, and IGFBP-3 levels were all significantly elevated in the high-dose group compared to the low-dose group (P<0.05). No significant difference in adverse reaction incidence was observed between high-(9.5%) and low-dose (8.8%) groups (P>0.05). Conclusions: Higher doses of rhGH can provide improved curative effects in patients with idiopathic short stature, likely via elevated levels of IGF-1 and IGFBP-3. [ABSTRACT FROM AUTHOR]

Objective: To estimate the mean prevalence of periodontal pathology of adjacent second molars (A-M2s) to third molars (M3s) and identify related confounding factors. Methods: Studies published before August 2020 were systematically searched in the Cochrane Library, EMBASE and MEDLINE databases. We included cross-sectional studies that evaluated the periodontal pathology of A-M2s based on clinical or radiographic examinations at the molar level. Studies employing similar periodontal parameters were pooled. Clinical attachment loss = 3 mm, alveolar bone loss = 3 mm or = 20% root length were defined as early periodontal defects, and at least one site with probing depth = 5 mm was considered as deep periodontal pockets around A-M2s in the data synthesis. Results: Nine studies (14,749 M3s) were ultimately included in the meta-analysis. On average, 19% of A-M2s showed distal early periodontal defects with the presence of M3s (95% confidence interval [95% CI] 9%-35%). Subgroup analyses suggested the prevalence was 32% (95% CI 16%-54%) in the mandible, and the prevalence was higher with nonimpacted M3s (25%, 95% CI 12%-47%) than with impacted M3s (19%, 95% CI 10%-35%). Additionally, the pooled prevalence for deep periodontal pockets around A-M2s was 52% (95% CI 39%-64%). Subgroup analyses suggested the prevalence was higher in the mandible (62%, 95% CI 45%-76%) than in the maxilla (43%, 95% CI 31%-56%), and for nonimpacted M3s the prevalence reached 50% (95% CI 36%-64%). Conclusion: The presence of M3s, especially mandibular and nonimpacted M3s, negatively affects the periodontal status of A-M2s. [ABSTRACT FROM AUTHOR]

Radiotherapy (RT) is a frequently used means in clinical tumor treatment. The outcome of RT varies, however, to a great extent, due to RT resistance or intolerable dose, which might be resolved by the development of radio-sensitizing strategies. Here, we report redox-sensitive iodinated polymersomes (RIP) carrying histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA, vorinostat), as a new dual-functional nano-radiosensitizer for breast cancer radiotherapy. SAHA-loaded RIP (RIP-SAHA) with a size of about 101nm exhibited good colloidal stability while the reduction-activated release of SAHA, giving rise to better antitumor effect to 4T1 breast carcinoma cells than free SAHA. Accordingly, RIP-SAHA combined with a 4Gy dose of X-ray radiation led to significantly enhanced suppression of 4T1 cells compared with SAHA combined 4Gy of X-ray radiation, as a result of enhanced DNA damage and impeded DNA damage repair. The pharmacokinetics and biodistribution studies by single-photon emission computed tomography (SPECT) with 125I-labeled SAHA (125I-SAHA) showed a 17.3-fold longer circulation and 237.7-fold better tumor accumulation of RIP-SAHA over SAHA. The systemic administration of RIP-SAHA greatly sensitized radiotherapy of subcutaneous 4T1 breast tumors and brought about significant inhibition of tumor growth, without causing damages to major organs, compared with radiotherapy alone. RIP not only enhanced SAHA delivery but also acted as a radiosensitizer. RIPSAHA emerges as a smart dual-functional nano-radiosensitizer to effectively enhance tumor radiotherapy. [ABSTRACT FROM AUTHOR]

Silkworm is an important economic insect and very sensitive to pesticides. The pollution of pyriproxyfen leads to the detriment of the growth and cocooning of the silkworm, which seriously affects the development of sericulture. Results showed that at 24 h and 48 h after pyriproxyfen exposure, the relative expressions of digestive enzyme genes, oxidative phosphorylation genes, and antioxidant enzyme genes were reduced, suggesting the inhibition of pyriproxyfen on digestion and absorption, energy metabolism, and antioxidation in the midgut of a silkworm. However, the activities of detoxification enzymes and the expressions of detoxification-related genes were elevated in the midgut of silkworm after 24 h and 48 h of pyriproxyfen exposure, indicating the enhanced resistance of silkworm to pyriproxyfen. In general, this study revealed the silkworm's midgut response to pyriproxyfen and provided an important reference to understand the metabolic mechanism of pyriproxyfen in this insect. [ABSTRACT FROM AUTHOR]

The hysteresis loops and the magnetic reversal process of exchange-coupled Nd2Fe14B/α″-Fe16N2 bilayers, with a deviation angle of easy axis β from the applied magnetic field direction and different thicknesses of soft phase were investigated using the object-oriented micromagnetic framework. Results show that a deviation of easy axis in Nd2Fe14B/α″-Fe16N2 bilayers can affect the magnetic properties and significantly decrease the energy product and coercivity. The remanence increases, whereas the coercivity of the bilayers drops monotonically, with the increase in thickness of the soft magnetic layer (Ls). A well-oriented bilayer with Ls = 4 nm exhibits the largest maximum energy product (i.e., 881.67 kJ/m³), which is larger than that of the other common exchange spring materials that we calculated. Therefore, the proposed nanocomposite magnet is one of the most promising exchange spring systems to achieve maximum energy product. [ABSTRACT FROM AUTHOR]

The age-stage, two-sex life table was used to determine the effects of the novel ketoponic acid insecticide, spirotetramat, on Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) at a concentration of 30mg/L. The results showed that the egg hatch rate was significantly decreased. In addition, the duration of the egg stage was decreased by one day compared to the control cohort. The preadult duration, adult longevity, pre-oviposition period, and total pre-oviposition period of the spirotetramat-treated cohort were all shortened, as were the preadult survival rate, mean fecundity, and net reproductive rate. No significant differences were found in the finite rate of increase and intrinsic rate of increase between the spirotetramat-treated and control cohorts. However, our population projection based on basic data (i.e., survival rate, developmental rate, and fecundity) showed that the population size of F. occidentalis was lower in the spirotetramat-treated cohort than in the control cohort. Our results showed that treatment with spirotetramat (30mg/L) suppressed the population growth of F. occidentalis. Furthermore, by using basic life table data to project population growth, we were able to distinguish differences between the projected growth of the two populations despite not finding a significant difference in their intrinsic rate of increase or finite rate of increase. This novel insecticide can be considered as a suitable candidate for inclusion in pest management programmes. [ABSTRACT FROM AUTHOR]

Objective: To detect COPD differential circular RNA and explain its possible functions and regulatory mechanisms using RNA sequencing technology. Methods: Collect the peripheral blood of 5 COPD patients and 5 normal people, separate mononuclear cells, de-linearize to obtain circular RNA, and construct a library for sequencing. The difference is statistically analyzed with the Foldchange absolute value ≥2 and P<0.05, and cluster analysis of the possible functions and pathways of circular RNA by host gene function and signal pathway. Results: Sequencing identified 1,215 circular RNAs and a total of 187 circular RNAs with significant differences, of which 107 were up-regulated and 80 were down-regulated; Enrichment of host genes from circular RNA The most obvious functions of enrichment are: cell process, cell and binding. The first three pathways with the most obvious changes are non-homologous end junction pathway, iron death pathway, and FoxO signaling pathway. Conclusion: RNA sequencing has discovered the significantly changed circular RNA in COPD, as well as the functions and pathways that circular RNA may regulate, providing a theoretical basis for understanding the pathogenesis of COPD from a new perspective of circular RNA. [ABSTRACT FROM AUTHOR]

Background: Plant allelopathy refers to the release of chemicals from plants or microorganisms into the environment, may have direct or indirect, beneficial or harmful effects on other plants or microorganisms. When plants grow in an unfavorable environment, more allelochemicals will be secreted and the expression of allelopathic effects will increase, giving plants a certain competitive advantage. Hairy vetch is one of the most promising allelopathic crops and the aqueous extract of hairy vetch has an inhibitory effect on the root length and seedling height of grass crops. The current study aimed to study the allelopathic effect of hairy vetch on alfalfa, and exploring an ecological method to remove the root system of alfalfa. Methods: In this experiment, the allelopathic effects of the seeds, stems and leaves, roots extracts and root exudates (0, 3, 6, 9 and 12 mg·mL-1) on the seed germination and seedling growth of alfalfa were evaluated. And the main allelopathic substances from the stem and leaf extract were isolated and identified using high performance liquid chromatography- mass spectrometry (HPLC-MS). Result: The results showed that all of the extracts can inhibit alfalfa seed germination and seedling growth and stem and leaf extract had the strongest inhibitory effect, especially for inhibiting the root growth. A main allelochemical substance, o-coumaric acid, was screened out and the root length of alfalfa was completely inhibited at 1.6 mg·mL-1 of o-coumaric acid. The findings of these experiments show hairy vetch has strong allelopathic effect on alfalfa and o-coumaric acid is a chemical growth inhibitor. [ABSTRACT FROM AUTHOR]

Circular RNAs (circRNAs) have gained much attention for their crucial regulatory roles in human diseases and cancers. However, the role and the mechanism of circRNA ArfGAP with FG repeats 1 (circAGFG1) in nonsmall-cell lung cancer (NSCLC) are still largely unknown. circAGFG1 was highly expressed in NSCLC, and high expression of circAGFG1 was closely related to the low survival rate of NSCLC patients. circAGFG1 knockdown inhibited the proliferation, migration, and invasion and promoted the apoptosis of NSCLC cells. circAGFG1 bound to miR-28-5p in NSCLC cells, and circAGFG1 promoted NSCLC progression partly through sponging miR-28-5p in vitro. HIF-1α was a target of miR-28-5p, and miR-28-5p overexpression-mediated influences in NSCLC cells were partly overturned by the addition of HIF-1α overexpression plasmid. circAGFG1/miR-28-5p/HIF-1α axis regulated cellular glycolytic metabolism in NSCLC cells. circAGFG1 silencing restrained the xenograft tumor growth in vivo. circAGFG1 promoted the proliferation, migration, and invasion and suppressed the apoptosis of NSCLC cells through accelerating the glycolysis via miR-28-5p/HIF-1α axis. [ABSTRACT FROM AUTHOR]

Objective: To study the expression of miR-186 in serum exosomes of patients with chronic obstructive pulmonary disease, and to explore its clinical significance with chronic obstructive pulmonary disease. Methods: The peripheral blood sera of 53 COPD patients and 53 normal persons in our hospital were collected. Extraction of exosomes and electron microscopy to identify the characteristics of exosomes. RT-PCR method was used to detect the miR-186 expression level in serum exosomes. And statistical analysis of the expression difference between the COPD group and the normal group, alysis of the correlation between miR-186 and FEV1% predicted value, and ROC curve analysis of the diagnostic value of serum exosome miR-186 in COPD. Results: The relative expression of miR-186 in serum exosomes of COPD group was 3.24 ± 0.14, which was significantly higher than that of normal control group 1.03 ± 0.04 (t = 15.47, P <0.001). The expression level of miR-186 was negatively correlated with the predicted FEV1% of COPD patients (r = -0.5024, p <0.0001). The area under the ROC curve between miR-186 and COPD patients was 0.8409 (95% confidence interval: 0.6875 -0.9943). Conclusion: The high expression of miR-186 in serum exosomes may be a risk factor and may be used as a diagnostic indicator of COPD. [ABSTRACT FROM AUTHOR]

Objective: To screen abnormally expressed lncRNAs in peripheral blood mononuclear cells from patients with COPD. Methods: The peripheral blood of 3 COPD patients and 3 normal controls were collected from our hospital, mononuclear cells were isolated, RNA was extracted and then transcriptome sequencing was performed. The expression difference between the two groups of samples was calculated based on p <0.05 and | log2FC |> 1.Plot the difference lncRNA heat map and volcano map. The Lncpro database may predict mRNAs regulated by differential lncRNA, and perform the GO function and KEGG signaling clustering. Results: There were 67 lncRNAs between the COPD group and the control group that met the difference of p <0.05 and | log2FC |> 1, of which 33 were up-regulated and 34 were down-regulated. Between the two groups. The target genes are mainly enriched in GO functions: regulatory functions of multicellular biological processes, regulatory functions of development processes, structured morphogenesis functions, system development functions, and development process functions. Target genes are mainly enriched in KEGG signaling pathways: multi-species apoptotic pathway, TGF-β signaling pathway, complement and coagulation cascade pathway, colorectal cancer pathway and apoptosis pathway. Conclusion: Our results provide general information and possible regulatory functions and pathways of lncRNA expression changes in peripheral blood mononuclear cells of COPD, which may help clarify the underlying mechanism of COPD. [ABSTRACT FROM AUTHOR]

BACKGROUND: Colleges and universities are not only the cradle of training talents, but also the base of incubating innovation. The influence on ranking reflects the strength of universities, and also represents the social recognition of universities. OBJECTIVE: It is of great significance to identify and analyze the key factors that affect the university ranking. METHODS: In this paper, the DANP method is used to identify the key factors of university influence. It is considered that the amount of alumni donation, world ranking, media attention of alumni, dual micromedia communication and industry media attention are the key factors that enhance universities' social influence. CONCLUSIONS: The conclusion of this study enriches the theory of university evaluation, innovates the research methods of university evaluation, and puts forward a precise index adjustment scheme to guide universities to strengthen their own influence construction, which has important theoretical and practical significance for the development of university edifice. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression of lncRNA SNHG3 in peripheral blood mononuclear cells of patients with chronic obstructive pulmonary disease (COPD), and to explore its clinical significance with chronic obstructive pulmonary disease. Methods: Mononuclear cells were collected from 120 patients with COPD and 110 normal controls. The expression of lncRNA SNHG3 in peripheral blood mononuclear cells was detected by RT-PCR and the difference between COPD group and normal group, and the difference between mild, moderate, severe, extremely severe COPD and health volunteers was analyzed. Results: The relative expression of SNHG3 in peripheral blood mononuclear cells of COPD patients was 1.52 ± 0.52, which was lower than that of the normal control group (2.51 ± 0.59) (P < 0.001). The relative expression of SNHG3 in peripheral blood mononuclear cells of mild COPD was 2.16 ± 0.23, which was higher than that of moderate group (1.55 ± 0.10) (P < 0.001), severe group 1.19 ± 0.11 (P< 0.001), and extremely severe group 0.89 ± 0.06(P < 0.001). In addition, lncRNA SNHG3 can bind to miR-186 and regulate its expression. Conclusions: The expression of lncRNA SNHG3 in peripheral blood mononuclear cells of COPD patients may be a risk factor for COPD and an indicator of the severity of COPD patients. The lncRNA SNHG3/miR-186 regulatory network may play an important role in the development of COPD. [ABSTRACT FROM AUTHOR]