RNA sequencing to investigate the difference of circular RNA expression in peripheral blood mononuclear cells of patients with COPD.

Objective: To detect COPD differential circular RNA and explain its possible functions and regulatory mechanisms using RNA sequencing technology. Methods: Collect the peripheral blood of 5 COPD patients and 5 normal people, separate mononuclear cells, de-linearize to obtain circular RNA, and construct a library for sequencing. The difference is statistically analyzed with the Foldchange absolute value ≥2 and P<0.05, and cluster analysis of the possible functions and pathways of circular RNA by host gene function and signal pathway. Results: Sequencing identified 1,215 circular RNAs and a total of 187 circular RNAs with significant differences, of which 107 were up-regulated and 80 were down-regulated; Enrichment of host genes from circular RNA The most obvious functions of enrichment are: cell process, cell and binding. The first three pathways with the most obvious changes are non-homologous end junction pathway, iron death pathway, and FoxO signaling pathway. Conclusion: RNA sequencing has discovered the significantly changed circular RNA in COPD, as well as the functions and pathways that circular RNA may regulate, providing a theoretical basis for understanding the pathogenesis of COPD from a new perspective of circular RNA. [ABSTRACT FROM AUTHOR]