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The reduction of detector dead time represents an enabling factor in several photon counting applications. In this work, we investigate the free-running operation of reach-through single-photon avalanche diodes (SPADs) at ultra-low dead times. By employing a fast active quenching circuit with direct bonding to the detector, we are able to achieve a 10 ns dead time with a thick SPAD by Excelitas, still maintaining extremely low afterpulsing probabilities (below 1.5%). [ABSTRACT FROM AUTHOR]

Time-Correlated Single-Photon Counting (TCSPC) is an excellent technique used in a great variety of scientific experiments to acquire exceptionally fast and faint light signals. Above all, in Fluorescence Lifetime Imaging (FLIM), it is widely recognized as the gold standard to record sub-nanosecond transient phenomena with picosecond precision. Unfortunately, TCSPC has an intrinsic limitation: to avoid the so-called pile-up distortion, the experiments have been historically carried out, limiting the acquisition rate below 5% of the excitation frequency. In 2017, we demonstrated that such a limitation can be overcome if the detector dead time is exactly matched with the excitation period, thus paving the way to unprecedented speedup of FLIM measurements. In this paper, we present the first single-channel system that implements the novel proposed methodology to be used in modern TCSPC experimental setups. To achieve this goal, we designed a compact detection head, including a custom single-photon avalanche diode externally driven by a fully integrated Active Quenching Circuit (AQC), featuring a finely tunable dead time and a short reset time. The output timing signal is extracted by using a picosecond precision Pick-Up Circuit (PUC) and fed to a newly developed timing module consisting of a mixed-architecture Fast Time to Amplitude Converter (F-TAC) followed by high-performance Analog-to-Digital Converters (ADCs). Data are transmitted in real-time to a Personal Computer (PC) at USB 3.0 rate for specific and custom elaboration. Preliminary experimental results show that the new TCSPC system is suitable for implementing the proposed technique, achieving, indeed, high timing precision along with a count rate as high as 40 Mcps. [ABSTRACT FROM AUTHOR]

In this paper we present a new technology for the fabrication of Single Photon Avalanche Diodes (SPADs) aimed at combining the advantages of thin and thick SPADs. The new detector is manufactured in a thick epitaxial layer designed to improve the Photon Detection Efficiency (PDE) in the red and near-infrared range while maintaining a good timing resolution. Experimental characterization of the new red-enhanced SPAD (RE-SPAD) confirmed a significant improvement of the PDE compared with thin SPADs; for example the PDE at a wavelength of 800 nm has increased from 15% to about 40%. Nevertheless the temporal resolution is still good, with a timing jitter of about 90 ps FWHM. In the same operating conditions the dark count rate is comparable with the one attainable with a thin SPAD (e.g. less than 25 cps for a 50 µm diameter device cooled down to −5°C). Moreover, being planar, the new technology is compatible with the fabrication of arrays of detectors. [ABSTRACT FROM PUBLISHER]

Recently developed Active Quenching Circuits (AQCs) with fast-gating capabilities allow us to control a single photon avalanche diode with gate windows in the nanosecond and sub-nanosecond range, thus paving the way to advanced applications, especially in the field of time-correlated single photon counting. In this scenario, an accurate measurement of the time needed by the AQC to turn-on the detector is of utmost importance. Indeed, it permits us to evaluate the impact of the system in specific applications and provides a tool to designers to understand AQC limitations and to enhance its performance. Here we propose a simple non-invasive technique to accurately measure the time needed by a gated system to turn on the detector. The effectiveness of the measure has been proved on a gated system, and results have been compared to those obtained starting from the distribution of recorded photons under constant illumination, which is a widely used approach in the literature. The great advantage of the proposed approach is that it avoids typical artifacts that affect other kinds of measurements. [ABSTRACT FROM AUTHOR]

Multi-dimensional time correlated single-photon counting has reached a prominent position among analytical techniques employed in the medical and biological fields. The development of instruments able to perform temporal and spectral fluorescence analysis (sFLIM) at the same time is limited by the performance of single-photon detectors, since currently available arrays cannot simultaneously satisfy all the requirements. To face this rising quest, a fully-parallel eight-channel module, based on a monolithic single-photon avalanche diode (SPAD) array with great temporal resolution, high photon detection efficiency and low dark count rate, has been designed and fabricated. The system relies on a novel architecture of the single pixel, based on the integration of the timing pick-up circuit next to the photodetector, making the negative effects of electrical and optical crosstalk on photon timing performance negligible. To this end, the custom technological process used to fabricate the SPAD has been modified, allowing the integration of MOS transistors without impairing the structure and the performance of the detector. The single channel is complemented by an external active quenching circuit, fabricated in a standard CMOS technology, that ensures high maximum counting rate (>5 MHz) and low after-pulsing (<2%). Finally, the output timing signals are read and conditioned by proper CMOS electronics. The complete system shows a very good temporal resolution of about 45 ps (FWHM). [ABSTRACT FROM AUTHOR]

After a brief review of the physics of photon detection in single photon avalanche diode (SPAD) devices, in this paper we will outline the principle of operation of a model we developed with the aim of calculating both photon detection efficiency (PDE) and temporal response (TR) of these detectors. Then we will apply the model to the devices currently available in order to critically analyze some experimental results. We will show in particular how the use of the model allows us to gain a better understanding of the influence of each device parameter in determining both the PDE and the TR. Finally we will discuss some modifications that can be applied to the device structure in order to overcome such limitations. Their effectiveness in improving both the PDE and the TR will be investigated by means of the aforementioned model. The aim is to provide the reader with an insight of which performances can be expected in the next few years if a strong development of the SPAD structure is pursued. [ABSTRACT FROM AUTHOR]

Recent advances in fluorescence measurements for single molecule spectroscopy, genomics, proteomics and medical diagnostics require single-photon detectors with high quantum efficiency in the extended red spectral range (from 600 nm to 900 nm) and low noise (<50 kc s-1). Amongst industrial production, avalanche photodiodes with large diameter (up to 500 µm) are available which can work in Geiger mode (GM) with good photon detection efficiency (higher than 20%) and some of them also with quite low dark counting rate. They allow one to attain high collection efficiency with simple optical systems. However, they work at high bias voltage (over 200 V) with high power dissipation and are easily damaged by exposure to intense light. A detector carrier module has been designed for safe operation and full performance exploitation of any silicon device suitable for GM operation, with any breakdown voltage up to 480 V. Efficient photon counting and timing is achieved in a very compact module by means of an integrated active-quenching circuit (iAQC) with fast time pickup. Catastrophic failure is avoided by a dedicated monitor and safety circuit, even in the case of exposure to sunlight. [ABSTRACT FROM AUTHOR]

The evolution of solid-state avalanche detectors of single optical photons is outlined and the issues for further progress are discussed. Physical phenomena that underlay the operation of the single-photon avalanche diodes (SPAD) and determine the performance are considered and their role is assessed (detection efficiency; dark-counting rate; afterpulsing; photon timing resolution; etc.). The main technological issues that hamper the development of detectors with wide sensitive area and of array detectors with high filling factor are illustrated. Silicon SPADs are the main focus of attention; infrared-sensitive SPADs in germanium and in compound semiconductors are also dealt with. The role of the active-quenching circuits (AQC) is assessed and the evolution is outlined up to integrated AQCs, which offer the prospect of monolithic integration of complete photon counter instruments. [ABSTRACT FROM AUTHOR]

In this paper, we describe a novel solution to increase the speed of Time-Correlated Single Photon Counting (TCSPC) measurements by almost an order of magnitude while providing, in principle, zero distortion regardless of the experimental conditions. Typically, the relatively long dead time associated with the conversion electronics requires a proper tune of the excitation power in order to avoid distortions of the reconstructed waveform due to pileup and counting loss. As a result, the maximum operating rate of a TCSPC channel is now limited between 1% and 5% of the excitation frequency, thus leading to relatively long acquisition times. We show that negligible distortion (below 1%) is guaranteed if the dead time associated with the converter is kept below the dead time of the detector, and at the same time the detector dead time is matched to the duration of the excitation period. In this way, unprecedented high-speed operation is possible. In this paper, we provide a theoretical analysis of the technique, including the main non-idealities which are introduced by a generic physical implementation. The results are supported by both numerical simulations and analytical calculations. [ABSTRACT FROM AUTHOR]

Time-Correlated Single Photon Counting (TCSPC) is a very efficient technique for measuring weak and fast optical signals, but it is mainly limited by the relatively "long" measurement time. Multichannel systems have been developed in recent years aiming to overcome this limitation by managing several detectors or TCSPC devices in parallel. Nevertheless, if we look at state-of-the-art systems, there is still a strong trade-off between the parallelism level and performance: the higher the number of channels, the poorer the performance. In 2013, we presented a complete and compact 32 x 1 TCSPC system, composed of an array of 32 single-photon avalanche diodes connected to 32 time-to-amplitude converters, which showed that it was possible to overcome the existing trade-off. In this paper, we present an evolution of the previous work that is conceived for high-throughput fluorescence lifetime imaging microscopy. This application can be addressed by the new system thanks to a centralized logic, fast data management and an interface to a microscope. The new conceived hardware structure is presented, as well as the firmware developed to manage the operation of the module. Finally, preliminary results, obtained from the practical application of the technology, are shown to validate the developed system. [ABSTRACT FROM AUTHOR]

In recent years, lifetime measurements by means of the Time Correlated Single Photon Counting (TCSPC) technique have led to a significant breakthrough in medical and biological fields. Unfortunately, the many advantages of TCSPC-based approaches come along with the major drawback of a relatively long acquisition time. The exploitation of multiple channels in parallel could in principle mitigate this issue, and at the same time it opens the way to a multi-parameter analysis of the optical signals, e.g., as a function of wavelength or spatial coordinates. The TCSPC multichannel solutions proposed so far, though, suffer from a tradeoff between number of channels and performance, and the overall measurement speed has not been increased according to the number of channels, thus reducing the advantages of having a multichannel system. In this paper, we present a novel readout architecture for bi-dimensional, high-density Single Photon Avalanche Diode (SPAD) arrays, specifically designed to maximize the throughput of the whole system and able to guarantee an efficient use of resources. The core of the system is a routing logic that can provide a dynamic connection between a large number of SPAD detectors and a much lower number of high-performance acquisition channels. A key feature of our smart router is its ability to guarantee high efficiency under any operating condition.q [ABSTRACT FROM AUTHOR]

This article discusses 35 ps time resolution at room temperature with large area single photon avalanche diodes. Specially designed avalanche photodetectors operated in Geiger-mode, i.e. biased at voltage higher than the breakdown level, can detect single photons. These devices are called single photon avalanche diodes (SPADs). SPADs have been successfully used in photon counting and timing measurements for a wide range of emerging scientific applications such as fluorescence analysis in the life sciences, quantum cryptography, and profilometry of remote objects with optical radar techniques.

Time-Correlated Single Photon Counting (TCSPC) has been long recognized as the most sensitive method for fluorescence lifetime measurements, but often requiring "long" data acquisition times. This drawback is related to the limited counting capability of the TCSPC technique, due to pile-up and counting loss effects. In recent years, multi-module TCSPC systems have been introduced to overcome this issue. Splitting the light into several detectors connected to independent TCSPC modules proportionally increases the counting capability. Of course, multi-module operation also increases the system cost and can cause space and power supply problems. In this paper, we propose an alternative approach based on a new detector and processing electronics designed to reduce the overall system dead time, thus enabling efficient photon collection at high excitation rate. We present a fast active quenching circuit for single-photon avalanche diodes which features a minimum dead time of 12.4 ns. We also introduce a new Time-to-Amplitude Converter (TAC) able to attain extra-short dead time thanks to the combination of a scalable array of monolithically integrated TACs and a sequential router. The fast TAC (F-TAC) makes it possible to operate the system towards the upper limit of detector count rate capability (~80 Mcps) with reduced pile-up losses, addressing one of the historic criticisms of TCSPC. Preliminary measurements on the F-TAC are presented and discussed. [ABSTRACT FROM AUTHOR]

A new algorithm for controlling the temperature of a thermoelectric cooler is proposed. Unlike a classic proportional-integral-derivative (PID) control, which computes the bias voltage from the temperature error, the proposed algorithm exploits the linear relation that exists between the cold side’s temperature and the amount of heat that is removed per unit time. Since this control is based on an existing linear relation, it is insensitive to changes in the operating point that are instead crucial in classic PID control of a non-linear system. [ABSTRACT FROM AUTHOR]

The minimization of Single Photon Avalanche Diodes (SPADs) dead time is a key factor to speed up photon counting and timing measurements. We present a fully integrated Active Quenching Circuit (AQC) able to provide a count rate as high as 100 MHz with custom technology SPAD detectors. The AQC can also operate the new red enhanced SPAD and provide the timing information with a timing jitter Full Width at Half Maximum (FWHM) as low as 160 ps. [ABSTRACT FROM AUTHOR]

Fluorescence correlation spectroscopy (FCS) is a well-established technique to study binding interactions or the diffusion of fluorescently labeled biomolecules in vitro and in vivo. Fast FCS experiments require parallel data acquisition and analysis which can be achieved by exploiting a multi-channel Single Photon Avalanche Diode (SPAD) array and a corresponding multi-input correlator. This paper reports a 32-channel FPGA based correlator able to perform 32 auto/cross-correlations simultaneously over a lag-time ranging from 10 ns up to 150 ms. The correlator is included in a 32 x 1S P A D array module, providing a compact and flexible instrument for high throughput FCS experiments. However, some inherent features of SPAD arrays, namely afterpulsing and optical crosstalk effects, may introduce distortions in the measurement of auto- and cross-correlation functions. We investigated these limitations to assess their impact on the module and evaluate possible workarounds. [ABSTRACT FROM AUTHOR]

Excellent performance of custom technology SPAD detectors have been widely demonstrated in recent years. Low‐jitter timing measurements with these detectors require front end electronics able to sense the avalanche current at a very low level when the multiplication process is still confined in a very small area around the photon absorption point. Best in class results (35 ps full width at half maximum) have been obtained with discrete circuits not suitable to be used in densely integrated systems of SPAD arrays required by modern demanding applications. A new fully integrated front end able to read out the avalanche current with a timing jitter as low as 32 ps and suitable to be exploited with SPAD arrays is presented. [ABSTRACT FROM AUTHOR]

Nowadays, an increasing number of applications require high-performance analytical instruments capable to detect the temporal trend of weak and fast light signals with picosecond time resolution. The Time-Correlated Single-Photon Counting (TCSPC) technique is currently one of the preferable solutions when such critical optical signals have to be analyzed and it is fully exploited in biomedical and chemical research fields, as well as in security and space applications. Recent progress in the field of single-photon detector arrays is pushing research towards the development of high performance multichannel TCSPC systems, opening the way to modern time-resolved multi-dimensional optical analysis. In this paper we describe a new 8-channel high-performance TCSPC acquisition system, designed to be compact and versatile, to be used in modern TCSPC measurement setups. We designed a novel integrated circuit including a multichannel Time-to-Amplitude Converter with variable full-scale range, a D/A converter, and a parallel adder stage. The latter is used to adapt each converter output to the input dynamic range of a commercial 8-channel Analog-to-Digital Converter, while the integrated DAC implements the dithering technique with as small as possible area occupation. The use of this monolithic circuit made the design of a scalable system of very small dimensions (95 × 40 mm) and low power consumption (6 W) possible. Data acquired from the TCSPC measurement are digitally processed and stored inside an FPGA (Field-Programmable Gate Array), while a USB transceiver allows real-time transmission of up to eight TCSPC histograms to a remote PC. Eventually, the experimental results demonstrate that the acquisition system performs TCSPC measurements with high conversion rate (up to 5 MHz/channel), extremely low differential nonlinearity (<0.04 peak-to-peak of the time bin width), high time resolution (down to 20 ps Full-Width Half-Maximum), and very low crosstalk between channels. [ABSTRACT FROM AUTHOR]

Emerged as a solid state alternative to photo multiplier tubes (PMTs), single-photon avalanche diodes (SPADs) are nowadays widely used in the field of single-photon timing applications. Custom technology SPADs assure remarkable performance, in particular a 10 counts/s dark count rate (DCR) at low temperature, a high photon detection efficiency (PDE) with a 50% peak at 550 nm and a 30 ps (full width at half maximum, FWHM) temporal resolution, even with large area devices, have been obtained. Over the past few years, the birth of novel techniques of analysis has led to the parallelization of the measurement systems and to a consequent increasing demand for the development of monolithic arrays of detectors. Unfortunately, the implementation of a multidimensional system is a challenging task from the electrical point of view; in particular, the avalanche current pick-up circuit, used to obtain the previously reported performance, has to be modified in order to enable high parallel temporal resolution, while minimizing the electrical crosstalk probability between channels. In the past, the problem has been solved by integrating the front-end electronics next to the photodetector, in order to reduce the parasitic capacitances and consequently the filtering action on the current signal of the SPAD, leading to an improvement of the timing jitter at higher threshold. This solution has been implemented by using standard complementary metal-oxide-semiconductor (CMOS) technologies, which, however, do not allow a complete control on the SPAD structure; for this reason the intrinsic performance of CMOS SPADs, such as DCR, PDE, and afterpulsing probability, are worse than those attainable with custom detectors. In this paper, we propose a pixel architecture, which enables the development of custom SPAD arrays in which every channel maintains the performance of the best single photodetector. The system relies on the integration of the timing signal pick-up circuit next to the photodiode, achieved by modifying the technological process flow used for the fabrication of the custom SPAD. The pixel is completed by an external standard CMOS active quenching circuit, which assures stable timing performance at quite high count rate (>1 MHz). [ABSTRACT FROM AUTHOR]