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In preclinical research, histology images are produced using powerful optical microscopes to digitize entire sections at cell scale. Quantification of stained tissue relies on machine learning driven segmentation. However, such methods require multiple additional information, or features, which are increasing the quantity of data to process. As a result, the quantity of features to deal with represents a drawback to process large series or massive histological images rapidly in a robust manner. Existing feature selection methods can reduce the amount of required information but the selected subsets lack reproducibility. We propose a novel methodology operating on high performance computing (HPC) infrastructures and aiming at finding small and stable sets of features for fast and robust segmentation of high-resolution histological images. This selection has two steps: (1) selection at features families scale (an intermediate pool of features, between spaces and individual features) and (2) feature selection performed on pre-selected features families. We show that the selected sets of features are stables for two different neuron staining. In order to test different configurations, one of these dataset is a mono-subject dataset and the other is a multi-subjects dataset to test different configurations. Furthermore, the feature selection results in a significant reduction of computation time and memory cost. This methodology will allow exhaustive histological studies at a high-resolution scale on HPC infrastructures for both preclinical and clinical research. [ABSTRACT FROM AUTHOR]

We show how the weak field magneto-conductance can be used as a tool to characterize epitaxial graphene samples grown from the C or the Si face of silicon carbide, with mobilities ranging from 120 to 12 000 cm2/(V·s). Depending on the growth conditions, we observe anti-localization and/or localization, which can be understood in term of weak-localization related to quantum interferences. The inferred characteristic diffusion lengths are in agreement with the scanning tunneling microscopy and the theoretical model which describe the 'pure' mono-layer and bilayer of graphene [MacCann et al., Phys. Rev. Lett. 97, 146805 (2006)]. [ABSTRACT FROM AUTHOR]

A 37-year-old man presented with a 2-year history of left hip pain. Pretherapeutic imaging demonstrated a 4 cm osteoblastoma located in the intertrochanteric region of the proximal femur, surrounded by extensive bone marrow edema. After multidisciplinary meeting, percutaneous cryoablation was decided and performed under computed tomography guidance using three cryoprobes to match the exact size and shape of the tumor, resulting in complete resolution of symptoms. Magnetic resonance imaging follow-up demonstrated resolution of the bone marrow edema pattern and ingrowth of fat at the periphery of the ablation zone consistent with long-term healing of the tumor. [ABSTRACT FROM AUTHOR]

SALAS S., HUYNH T.-K., GIORGI R., DEVILLE J.-L., BOLLINI G., CURVALE G., BLESIUS A., GENTET J.-C., BUI B., BOUVIER C. & DUFFAUD F. (2011) European Journal of Cancer Care, 322-329 Limited information is available on clinical management of Flat Bone Osteosarcomas (FBOS). We retrospectively analysed prognostic factors and outcome. Twenty-eight patients were treated in our institution. Survival curves were obtained by the Kaplan-Meier method and compared with the log-rank test. The overall survival (OS) rates at 5 and 10 years were 52.4% and 45.8% respectively. The event-free survival (EFS) rates at 5 and 10 years were 41.5%. The factors influencing EFS in univariate analysis were location, metastatic disease at diagnosis, effect of neoadjuvant chemotherapy, histological response and adequate local tumour control. Location, metastatic disease at diagnosis, effect of neoadjuvant chemotherapy, histological response and local recurrence were statistically correlated with OS. Multivariate analysis retained metastatic disease at diagnosis as prognostic factors of EFS and OS. Our results suggest a more favourable outcome of FBOS as the use of a treatment scheme based on the protocols for long bone osteosarcomas. However, an adequate local treatment is essential to ensure a better outcome. [ABSTRACT FROM AUTHOR]

Tumour growth of vestibular schwannomas is still difficult to predict. The aim of our study was to determine whether any defined histopathological feature was correlated with the clinical course. We did a retrospective study with 69 paraffin-embedded tumours to establish whether the number of vessels, blood cells extravasation or degree of inflammation, all semi-quantitatively assessed, could be indicative of potential of growth. An immunohistochemical study was also performed with an endothelial marker CD34, the leukocyte common antigen CD45 and the estrogen and progesterone hormone receptors. All these parameters were correlated with patient's age, duration of symptoms (d), with a clinical growth index (CLI = tumour size/d). No clinical parameters proved to be predictive of tumour growth. Tumour size was significantly (p = 0.01) related to the number of vessels and we found a significant relationship between the clinical growth index (CLI) and total number of vessels, especially when duration of symptoms lasted less than 1 year (p < 0.001). However, we found no relationship between duration of symptoms or CLI and CD34 index. The degree of inflammation was significantly correlated (p = 0.007) with duration of symptoms when it lasted more than 1 year. The CD45 index and the semi-quantitative evaluation of the inflammation were well correlated (p = 0.001). No estrogen receptors antigenic site was detected and only seven tumours expressed progesterone receptor in a few cells without any significant clinical value. These results suggest that vessel density is determinant for sporadic acoustic neuroma growth especially for a short clinical course. [ABSTRACT FROM AUTHOR]

Primary epiphyseal subacute osteomyelitis (PESAO) caused by Mycobacterium species in young children is poorly recognized. We aimed to define the spectrum of this uncommon condition and to propose a novel diagnostic approach. We performed a systematic review of the literature on the PubMed website by selecting all reports of isolated infantile PESAO caused by Mycobacterium species since 1975. We identified 350 citations, of which 174 were assessed for eligibility based on title and abstract. The full text of 81 eligible citations was screened, and relevant data of 15 children under 4 years of age with mycobacterial PESAO were extracted. These data were pooled with those from our Institution. Data from 16 children were reviewed. The median age was 16 ± 7 months and the male:female ratio 1.7. The knee was the most common infection site (94%). The diagnosis of mycobacterial disease was delayed in all cases (range, 2 weeks to 6 months), and initially presumed by histology in 15 children (94%). Microbiologically proven diagnosis was confirmed by bone cultures in 8 of the 15 children (53%), and by specific PCR in 2 of the 3 culture-negative bone specimens (67%). Three children experienced long-term orthopedic complications despite surgical drainage and prolonged antimycobacterial regimens. All recently reported cases came from high-burden tuberculosis areas. Mycobacterium species contribute to the burden of infantile PESAO in endemic tuberculosis areas and may cause growth disturbances. We argue in favor of the early recognition of mycobacterial disease by specific molecular assays in children with infantile PESAO living in high-burden areas. [ABSTRACT FROM AUTHOR]

Mediterranean catchments in southern France are threatened by potentially devastating fast floods which are difficult to anticipate. In order to improve the skill of rainfall-runoff models in predicting such flash floods, hydrologists use data assimilation techniques to provide real-time updates of the model using observational data. This approach seeks to reduce the uncertainties present in different components of the hydrological model (forcing, parameters or state variables) in order to minimize the error in simulated discharges. This article presents a data assimilation procedure, the best linear unbiased estimator (BLUE), used with the goal of improving the peak discharge predictions generated by an event-based hydrological model Soil Conservation Service lag and route (SCS-LR). For a given prediction date, selected model inputs are corrected by assimilating discharge data observed at the basin outlet. This study is conducted on the Lez Mediterranean basin in southern France. The key objectives of this article are (i) to select the parameter(s) which allow for the most efficient and reliable correction of the simulated discharges, (ii) to demonstrate the impact of the correction of the initial condition upon simulated discharges, and (iii) to identify and understand conditions in which this technique fails to improve the forecast skill. The correction of the initial moisture deficit of the soil reservoir proves to be the most efficient control parameter for adjusting the peak discharge. Using data assimilation, this correction leads to an average of 12 % improvement in the flood peak magnitude forecast in 75 % of cases. The investigation of the other 25 % of cases points out a number of precautions for the appropriate use of this data assimilation procedure. [ABSTRACT FROM AUTHOR]

In northern Morocco are located most of the dams and reservoirs of the country, while this region is affected by severe rainfall events causing floods. To improve the management of the water regulation structures, there is a need to develop rainfall--runoff models to both maximize the storage capacity and reduce the risks caused by floods. In this study, a model is developed to reproduce the flood events for a 655 km² catchment located upstream of the 6th largest dam in Morocco. Constrained by data availability, a standard event-based model combining a SCS-CN (Soil Conservation Service Curve Number) loss model and a Clark unit hydrograph was developed for hourly discharge simulation using 16 flood events that occurred between 1984 and 2008. The model was found satisfactory to reproduce the runoff and the temporal evolution of floods, even with limited rainfall data. Several antecedent wetness conditions estimators for the catchment were compared with the initial condition of the model. Theses estimators include an antecedent discharge index, an antecedent precipitation index and a continuous daily soil moisture accounting model (SMA), based on precipitation and evapotranspiration. The SMA model performed the best to estimate the initial conditions of the event-based hydrological model (R² = 0.9). Its daily output has been compared with ASCAT and AMSR-E remote sensing data products, which were both able to reproduce with accuracy the daily simulated soil moisture dynamics at the catchment scale. This same approach could be implemented in other catchments of this region for operational purposes. The results of this study suggest that remote sensing data are potentially useful to estimate the soil moisture conditions in the case of ungauged catchments in Northern Africa. [ABSTRACT FROM AUTHOR]

The present study explores the application of a data assimilation (DA) procedure to correct the radar rainfall inputs of an event-based, distributed, parsimonious hydrological model. An extended Kalman filter algorithm was built on top of a rainfall-runoff model in order to assimilate discharge observations at the catchment outlet. This work focuses primarily on the uncertainty in the rainfall data and considers this as the principal source of error in the simulated discharges, neglecting simplifications in the hydrological model structure and poor knowledge of catchment physics. The study site is the 114 km² Lez catchment near Montpellier, France. This catchment is subject to heavy orographic rainfall and characterised by a karstic geology, leading to flash flooding events. The hydrological model uses a derived version of the SCS method, combined with a Lag and Route transfer function. Because the radar rainfall input to the model depends on geographical features and cloud structures, it is particularly uncertain and results in significant errors in the simulated discharges. This study seeks to demonstrate that a simple DA algorithm is capable of rendering radar rainfall suitable for hydrological forecasting. To test this hypothesis, the DA analysis was applied to estimate a constant hyetograph correction to each of 19 flood events. The analysis was carried in two different modes: by assimilating observations at all available time steps, referred to here as reanalysis mode, and by using only observations up to 3 h before the flood peak to mimic an operational environment, referred to as pseudo-forecast mode. In reanalysis mode, the resulting correction of the radar rainfall data was then compared to the mean field bias (MFB), a corrective coefficient determined using rain gauge measurements. It was shown that the radar rainfall corrected using DA leads to improved discharge simulations and Nash-Sutcliffe efficiency criteria compared to the MFB correction. In pseudo-forecast mode, the reduction of the uncertainty in the rainfall data leads to a reduction of the error in the simulated discharge, but uncertainty from the model parameterisation diminishes data assimilation efficiency. While the DA algorithm used is this study is effective in correcting uncertain radar rainfall, model uncertainty remains an important challenge for flood forecasting within the Lez catchment. [ABSTRACT FROM AUTHOR]

In Northern Morocco are located most of the dams and reservoirs of the country, while this region is affected by severe rainfall events causing floods. To improve the management of the water regulation structures, there is a need to develop rainfall-runoff models to both maximize the storage capacity and reduce the risks caused by floods. In this study, a model is developed to reproduce the flood events for a 655 km² catchment located upstream of the 6th largest dam of the Morocco. Constrained by data availability, a standard event-based model was developed for hourly discharge using 16 flood events that occurred between 1984 and 2008. The model was found satisfactory to reproduce the runoff and the temporal evolution of floods, even with limited rainfall data. Several antecedent wetness conditions estimators for the catchment were compared with the initial condition of the model. These estimators include the discharge of the previous days, the antecedent precipitation index and a continuous daily soil moisture accounting model (SMA). The SMA model performed the best to estimate the initial conditions of the model, with R² = 0.9. Its daily output has been compared with ASCAT and AMSR-E remote sensing data products, both were able to reproduce with accuracy the daily soil moisture dynamics at the catchment scale. This same approach could be implemented in other catchments of this region for operational purposes. The results of this study indicate the potential usefulness of remote sensing data to estimate the soil moisture conditions in the case of ungauged catchments in Northern Africa. [ABSTRACT FROM AUTHOR]

Rainfall-runoff models are crucial tools for the statistical prediction of flash floods and real-time forecasting. This paper focuses on a karstic basin in the South of France and proposes a distributed parsimonious event-based rainfall-runoff model, coherent with the poor knowledge of both evaporative and underground fluxes. The model combines a SCS runoff model and a Lag and Route routing model for each cell of a regular grid mesh. The efficiency of the model is discussed not only to satisfactorily simulate floods but also to get powerful relationships between the initial condition of the model and various predictors of the initial wetness state of the basin, such as the base flow, the Hu2 index from the Meteo-France SIM model and the piezometric levels of the aquifer. The advantage of using meteorological radar rainfall in flood modelling is also assessed. Model calibration proved to be satisfactory by using an hourly time step with Nash criterion values, ranging between 0.66 and 0.94 for eighteen of the twenty-one selected events. The radar rainfall inputs significantly improved the simulations or the assessment of the initial condition of the model for 5 events at the beginning of autumn, mostly in September--October (mean improvement of Nash is 0.09; correction in the initial condition ranges from --205 to 124 mm), but were less efficient for the events at the end of autumn. In this period, the weak vertical extension of the precipitation system and the low altitude of the 0 °C isotherm could affect the efficiency of radar measurements due to the distance between the basin and the radar (~60 km). The model initial condition S is correlated with the three tested predictors (R² > 0.6). The interpretation of the model suggests that groundwater does not affect the first peaks of the flood, but can strongly impact subsequent peaks in the case of a multi-storm event. Because this kind of model is based on a limited amount of readily available data, it should be suitable for operational applications. [ABSTRACT FROM AUTHOR]

The present study explores the application of a data assimilation (DA) procedure to correct the radar rainfall inputs of an event-based, distributed, parsimonious hydrological model. A simplified Kalman filter algorithm was built on top of a rainfall-runoff model in order to assimilate discharge observations at the catchment outlet. The study site is the 114 km² Lez Catchment near Montpellier, France. This catchment is subject to heavy orographic rainfall and characterized by a karstic geology, leading to flash flooding events. The hydrological model uses a derived version of the SCS method, combined with a Lag and Route transfer function. Because it depends on geographical features and cloud structures, the radar rainfall input to the model is particularily uncertain and results in significant errors in the simulated discharges. The DA analysis was applied to estimate a constant correction to each event hyetogram. The analysis was carried out for 19 events, in two different modes: re-analysis and pseudo-forecast. In both cases, it was shown that the reduction of the uncertainty in the rainfall data leads to a reduction of the error in the simulated discharge. The resulting correction of the radar rainfall data was then compared to the mean field bias (MFB), a corrective coefficient determined using ground rainfall measurements, which are more accurate than radar but have a decreased spatial resolution. It was shown that the radar rainfall corrected using DA leads to improved discharge simulations and Nash criteria compared to the MFB correction. [ABSTRACT FROM AUTHOR]

The factors controlling estuarine phytoplankton diversity and production are relatively well known in temperate systems. Less however is known about the factors affecting phytoplankton community distribution in tropical estuaries. This is surprising given the economic and ecological importance of these large, deltaic ecosystems, such as are found in South East Asia. Here we present the results from an investigation into the factors controlling phytoplankton distribution and phytoplankton-bacterial coupling in the Bach Dang Estuary, a sub-estuary of the Red River system, in Northern Vietnam. Phytoplankton diversity and primary and bacterial production, nutrients and metallic contaminants (mercury and organotin) were measured during two seasons: wet (July 2008) and dry (March 2009). Phytoplankton community composition differed between the two seasons with only a 2% similarity between July and March. The large spatial extent and complexity of defining the freshwater sources meant that simple mixing diagrams could not be used in this system. We therefore employed multivariate analyses to determine the factors influencing phytoplankton community structure. Salinity and suspended particulate matter were important factors in determining phytoplankton distribution, particularly during the wet season. We also show that phytoplankton community structure is probably influenced by the concentrations of mercury species (inorganic mercury and methyl mercury in both the particulate and dissolved phases) and of tri-, di, and mono-butyl tin species found in this system. Freshwater phytoplankton community composition was associated with dissolved methyl mercury and particulate inorganic mercury concentrations during the wet season, whereas, during the dry season, dissolved methyl mercury and particulate butyl tin species were important factors for the discrimination of the phytoplankton community structure. Phytoplankton-bacterioplankton coupling was also investigated during both seasons. In the inshore, riverine stations the ratio between bacterial production and dissolved primary production was high supporting the hypothesis that bacterial carbon demand is supported by allochthonous riverine carbon sources. The inverse was true in the offshore stations, where BP:DPP values were less than 1, potentially rejecting differences in primary production due to shifting phytoplankton community diversity. [ABSTRACT FROM AUTHOR]

A good knowledge of rainfall is essential for hydrological operational purposes such as flood forecasting. The objective of this paper was to analyze, on a relatively large sample of flood events, how rainfall-runoff modeling using an event-based model can be sensitive to the use of spatial rainfall compared to mean areal rainfall over the watershed. This comparison was based not only on the model's efficiency in reproducing the flood events but also through the estimation of the initial conditions by the model, using different rainfall inputs. The initial conditions of soil moisture are indeed a key factor for flood modeling in the Mediterranean region. In order to provide a soil moisture index that could be related to the initial condition of the model, the soil moisture output of the Safran-Isba-Modcou (SIM) model developed by Météo-France was used. This study was done in the Gardon catchment (545 km²) in South France, using uniform or spatial rainfall data derived from rain gauge and radar for 16 flood events. The event-based model considered combines the SCS runoff production model and the Lag and Route routing model. Results show that spatial rainfall increases the efficiency of the model. The advantage of using spatial rainfall is marked for some of the largest flood events. In addition, the relationship between the model's initial condition and the external predictor of soil moisture provided by the SIM model is better when using spatial rainfall, in particular when using spatial radar data with R² values increasing from 0.61 to 0.72. [ABSTRACT FROM AUTHOR]

In order to define specific markers for histogenesis of three well-characterized subgroups of human gliomas (pilocytic astrocytomas, glioblastoma multiforme and oligodendrogliomas), we studied the expression of relevant markers that characterize gliomagenesis, by immunohistochemistry and in situ hybridization. They include the intermediate filament proteins glial fibrillary acidic protein (GFAP), vimentin and nestin, the transcription factors Olig2, Nkx2.2 and Sox10, and the proteolipid protein transcripts plp/dm20. We show that the three major categories of human gliomas express a combinatorial profile of markers that gives new insights to their histogenesis and may help diagnosis. Pilocytic astrocytomas strongly express GFAP, vimentin, Olig2, Nkx2.2 and Sox10 but not nestin. In contrast, glioblastomas strongly express GFAP, vimentin and nestin but these tumours are heterogeneous regarding the expression of the transcription factors studied. Finally, in oligodendrogliomas, intermediate filament proteins are generally not observed whereas Olig2 was found in almost all tumour cells nuclei while only a subpopulation of tumour cells expressed Nkx2.2 and Sox10. [ABSTRACT FROM AUTHOR]

The IDEA project aimed to improve the assessment of incorporated radionuclides through developments of advanced in vivo and bioassay monitoring techniques and making use of such enhancements for improvements in routine monitoring. Many of these findings are not new in the sense that they are being already employed in advanced laboratories or for specialised applications. The primary goal was to categorise those new developments regarding their potential and eligibility for the routine monitoring community. Attention has been given to in vivo monitoring techniques with respect to detector characteristics and measurement geometry to improve measurement efficiency with special attention to low energy gamma emitters. Calibration—specifically supported by or through methods of numerical simulation—have been carefully analysed to reduce overall measurement uncertainties and explore ways to accommodate the individual variability based on characteristic features of a given person. For bioassay measurements at low detection limits, inductively coupled plasma mass spectroscopy offers significant advantages both in accuracy, speed, and sample preparation. Specifically, the determination of U and Th in urine and the associated models have been investigated. Finally, the scientific achievements have been analysed regarding their potential to offer benefits for routine monitoring. These findings will be presented in greater detail in other papers at this conference, whereas this paper intends to give an overview and put both the scientific achievements as well as the derived benefits into perspective. [ABSTRACT FROM AUTHOR]

Human gliomas including astrocytomas and oligodendrogliomas are defined as being composed of neoplastic astrocytes and oligodendrocytes respectively. Here, on the basis of in vitro functional assays, we show that gliomas contain a mixture of glial progenitor cells and their progeny. We have set up explant cultures from pilocytic astrocytomas, glioblastomas and oligodendrogliomas and studied antigens that characterize glial lineage, from the precursor cells (glial restricted precursors and oligodendrocyte-type2-astrocyte/oligodendrocyte precursor cells expressing the A2B5 ganglioside) to the differentiated cells (oligodendrocyte and type-1 and type-2 astrocytes). All tumoral explants contain A2B5+ cells and can generate migrating cells with distinctive functional properties according to glioma subtypes. In pilocytic astrocytomas, very few migrating cells are dividing and can differentiate in type-2 astrocytes or towards the oligodendrocyte lineage. In glioblastomas, most migrating cells are dividing, express A2B5 or glial fibrillary acid protein (GFAP) and can generate oligodendrocytes and type-1 and type-2 astrocytes in appropriate medium. Oligodendroglioma explants are made by actively dividing glial precursor cells expressing A2B5 or PSA-NCAM. Only few cells can migrate and differentiation towards oligodendrocyte lineage does not occur. Isolated A2B5+ cells from both glioblastomas and oligodendrogliomas showed similar genetic alterations as the whole tumour. Therefore, pilocytic astrocytomas contain slowly dividing oligodendrocyte-type2-astrocyte/oligodendrocyte precursor cells in keeping with their benign behaviour whereas both glioblastomas and oligodendrogliomas contain neoplastic glial restricted precursor cells. In oligodendrogliomas, these cells are trapped in undifferentiated and proliferating state. The precursor cells properties present in gliomas give new insight into their histogenesis and open up new avenues for research in the field of gliomagenesis. [ABSTRACT FROM AUTHOR]

Characterizes different rainfall/runoff processes in a small peri-urban catchment in Quito, Ecuador. Physiographic units of the watersheds; Measurement of infiltration point-processes; Variations in the runoff coefficients.

We report an anatomico-radiologic study of humerus, femur, and tibia from a case of total congenital afibrinogenemia. Juxtatrabecular hemorrhages occur mainly in metaphyses and seem to be related to normal lines of stress. They may lead to the formation of intraosseous cysts and to a remodelling of bone trabeculae. The radiologic lesions in a second case, diagnosed as congenital dysfibrinogenemia, are similar to those found in Case 1 (femoral trabeculae remodelling) but also resemble some alterations described in hemophilia (pseudotumor of the right iliac bone). Anatomic study of the lesions in Case 2 was not possible. The significance of these observations could be better defined by a more extended skeletal study (radiologic and when feasible anatomic) of patients with congenital clotting defects and especially with inherited disorders of the fibrinogen molecule. It would also be worthwhile investigating manifest or latent hemostatic disorders (particularly at the fibrinogen level) in patients with solitary or aneurysmal bone cysts, and even with bone infarct or unexplained trabecular remodelling. [ABSTRACT FROM AUTHOR]

This paper presents a coupled observation and modelling strategy aiming at improving the understanding of processes triggering flash floods. This strategy is illustrated for the Mediterranean area using two French catchments (Gard and Ardèche) larger than 2000 km². The approach is based on the monitoring of nested spatial scales: (1) the hillslope scale, where processes influencing the runoff generation and its concentration can be tackled; (2) the small to medium catchment scale (1-100 km²), where the impact of the network structure and of the spatial variability of rainfall, landscape and initial soil moisture can be quantified; (3) the larger scale (100-1000 km²), where the river routing and flooding processes become important. These observations are part of the HyMeX (HYdrological cycle in the Mediterranean EXperiment) enhanced observation period (EOP), which will last 4 years (2012-2015). In terms of hydrological modelling, the objective is to set up regional-scale models, while addressing small and generally ungauged catchments, which represent the scale of interest for flood risk assessment. Top-down and bottom-up approaches are combined and the models are used as "hypothesis testing" tools by coupling model development with data analyses in order to incrementally evaluate the validity of model hypotheses. The paper first presents the rationale behind the experimental set-up and the instrumentation itself. Second, we discuss the associated modelling strategy. Results illustrate the potential of the approach in advancing our understanding of flash flood processes on various scales. [ABSTRACT FROM AUTHOR]

We present molecular beam epitaxy growth of tensile-strained HgTe/CdTe thin films for realization of three-dimensional (3D) topological insulator structures. The growth temperature is investigated by looking at crystal quality using high-resolution x-ray diffraction, being found to be much lower than the usual surface temperature for low-cadmium-fraction HgCdTe material. The strain status of HgTe is checked as a function of thickness, and the first indication of strain relaxation is found to appear for thickness well below the critical thickness expected for this system. Surface and interface morphology are also investigated, and well-defined interfaces as well as atomically flat surfaces are demonstrated. Finally, transmission electron microscopy is used to image the material structure of a HgCdTe/HgTe/HgCdTe stack suitable for electronic transport experiments. [ABSTRACT FROM AUTHOR]

Background Pilocytic astrocytomas ( PAs) are characterized by an excellent prognosis although several factors of adverse outcome have been reported. The mitogen-activated protein kinase pathway plays a major role in their tumorigenesis. Aim To report a series of 148 PAs in children to define clinicopathological and biological prognostic factors. Methods Clinical data were collected from patient files and mail inquiry. Pathological specimens were centrally reviewed. The three major KIAA1549: BRAF fusion subtypes were analysed by reverse transcription - polymerase chain reaction ( RT-PCR) in a subset of 47 frozen cases and by fluorescence in situ hybridization on formalin-fixed paraffin-embedded tissue in 23 cases. Tumour location, age at surgery, extent of surgical removal, histological subtype and KIAA1549: BRAF fusion by RT-PCR were searched for prognostic significance. Results Pilomyxoid astrocytoma ( PMA) and the hypothalamo-chiasmatic ( H/ C) location were associated with a worse prognosis [ P < 0.001 for overall survival ( OS) and P = 0.001 for progression-free survival ( PFS)]. Patients who underwent complete surgical excision had a better OS ( P = 0.004) and a longer PFS ( P < 0.001) than the others. Age was also a strong prognostic factor for OS but not for PFS. Infants (<1 year) and young children (<3 years) had a much worse outcome than the others ( P < 0.001 and P = 0.004 respectively). KIAA1549: BRAF fusion status was not predictive of outcome. Conclusion This study highlights the good prognostic factors of PAs but H/ C PA remains a subgroup with dismal prognosis associated with young age, PMA variant and incomplete surgery. Search for KIAA1549: BRAF fusion in tumours with PA pattern is recommended even though the prognostic impact is still unclear. [ABSTRACT FROM AUTHOR]

Changes in environmental conditions and prokaryote physiology can strongly affect the dynamics of both the lysogenic and lytic bacteriophage replication cycles in aquatic systems. However, it remains unclear whether it is the nature, amplitude or frequency of these changes that alter the phage replication cycles. We performed an annual survey of three Mediterranean lagoons with contrasting levels of chlorophyll a concentration and salinity to explore how these cues and their variability influence either replication cycle. The lytic cycle was always detected and showed seasonal patterns, whereas the lysogenic cycle was often undetected and highly variable. The lytic cycle was influenced by environmental and prokaryotic physiological cues, increasing with concentrations of dissolved organic carbon, chlorophyll a, and the proportion of respiring cells, and decreasing with the proportion of damaged cells. In contrast, lysogeny was not explained by the magnitude of any environmental or physiological parameter, but increased with the amplitude of change in prokaryote physiology. Our study suggests that both cycles are regulated by distinct factors: the lytic cycle is dependent on environmental parameters and host physiology, while lysogeny is dependent on the variability of prokaryote physiology. This could lead to the contrasting patterns observed between both cycles in aquatic systems. [ABSTRACT FROM AUTHOR]

Background: Preoperative diffusion-weighted MRI (DW-MRI) has been described as an efficient method to differentiate good and poor responders to chemotherapy in osteosarcoma patients. A DW-MRI performed earlier during treatment could be helpful in monitoring chemotherapy. Objective: To assess the accuracy of DW-MRI in evaluating response to chemotherapy in the treatment of osteosarcoma, more specifically at mid-course of treatment. Materials and methods: This study was carried out on a prospective series of adolescents treated for long-bone osteosarcoma. MR examinations were performed at diagnosis (MRI-1), at mid-course of chemotherapy (MRI-2), and immediately before surgery (MRI-3). A DW sequence was performed using diffusion gradients of b0 and b900. The apparent diffusion coefficients (ADC1, ADC2, ADC3, respectively), their differentials (ADC2 − ADC1 and ADC3 − ADC1), and their variation (ADC2 − ADC1/ADC1 and ADC3 − ADC1/ADC1) were calculated for each of these three time points. Results: Fifteen patients were included. Patients with no increase in ADC showed a poor response to chemotherapy on their histology results. At mid-course, the three calculated values were significantly different between good and poor responders. ADC2 − ADC1 enabled us to detect, with 100% specificity, four out of seven of the poor responders. There was no significant difference in the values at MRI-3 between the two groups. Conclusion: DW-MRI performed both at baseline and mid-course of neoadjuvant chemotherapy is an efficient method to predict further histological response of osteosarcoma. This method could be used as an early prognostic factor to monitor preoperative chemotherapy. [ABSTRACT FROM AUTHOR]

Summary [ABSTRACT FROM AUTHOR]

The idea of the IDEA project aimed to improve assessment of incorporated radionuclides through developments of more reliable and possibly faster in vivo and bioassay monitoring techniques and making use of such enhancements for improvements in routine monitoring. In direct in vivo monitoring technique the optimum choice of the detectors to be applied for different monitoring tasks has been investigated in terms of material, size and background in order to improve conditions namely to increase counting efficiency and reduce background. Detailed studies have been performed to investigate the manifold advantageous applications and capabilities of numerical simulation method for the calibration and optimisation of in vivo counting systems. This calibration method can be advantageously applied especially in the measurement of low-energy photon emitting radionuclides, where individual variability is a significant source of uncertainty. In bioassay measurements the use of inductively coupled plasma mass spectrometry (ICP-MS) can improve considerably both the measurement speed and the lower limit of detection currently achievable with alpha spectrometry for long-lived radionuclides. The work carried out in this project provided detailed guidelines for optimum performance of the technique of ICP-MS applied mainly for the determination of uranium and thorium nuclides in the urine including sampling procedure, operational parameters of the instruments and interpretation of the measured data. The paper demonstrates the main advantages of investigated techniques in comparison with the performances of methods commonly applied in routine monitoring practice. [ABSTRACT FROM AUTHOR]

A GIS-based one-dimensional flood simulation model is presented and applied to the centre of the city of Nimes (Gard, France), for mapping flow depths or velocities in the streets network. The geometry of the one-dimensional elements is derived from the Digital Elevation Model (DEM). The flow is routed from one element to the next using the kinematic wave approximation. At the crossroads, the flows in the downstream branches are computed using a conceptual scheme. This scheme was previously designed to fit X-shaped pipes junctions, and has been modified here to fit X-shaped crossroads. The results were compared with the results of a two-dimensional hydrodynamic model based on the full shallow water equations. The comparison shows that good agreements can be found in the steepest streets of the study zone, but differences may be important in the other streets. Some reasons that can explain the differences between the two models are given and some research possibilities are proposed. [ABSTRACT FROM AUTHOR]

Background. Synovial cysts represent an uncommon and probably underestimated pathological entity of the degenerative lumbar spine. The authors report a retrospective analysis of the clinical presentation, radiological studies and operative findings in 77 patients surgically treated for symptomatic lumbar synovial cysts at their institution. Materials and method. Between January 1992 and June 1998, a total of 77 patients presenting with symptomatic lumbar synovial cysts were operated on in the author’s department. Operative procedure, complications, results and pathological findings were correlated with preoperative assessment. There were 41 men and 36 women with an average age of 63 years (range 44–90 years). Results. On the basis of their symptom complex on presentation, two populations were identified: patients who presented with a single radicular pain (group I = 51 patients), and patients who presented with bilateral neurogenic claudication (group II = 26 patients). Neurological examination on presentation demonstrated motor deficit (12%), sensory loss (26%) and reflex changes (35%). Degenerative disc disease and facet joint osteoarthritis was a frequent finding in patients with pre-operative MRI. Facet joint orientation was >45° in 76.6% of patients. Preoperative spondylolisthesis was found in 48% on radiological studies. All the patients were treated surgically with resection of the cyst. No fusion was performed as a first line procedure. However subsequent fusion was necessary in one patient who developed symptomatic spondylolisthesis. Mean follow-up period was of 45 months ranging from 18 to 105 months. Only one recurrence occurred during the follow-up period. An excellent or good functional outcome was seen in 97.4% of cases, and 89% of the patients with motor deficit recovered. Conclusions. Surgical resection of lumbar synovial cysts is an effective treatment associated with very low morbidity. Synovial cysts are associated with increased grade and frequency of facet joint asteoarthritis but not with increased grade or frequency of degenerative disc disease compared with patients without cysts. In the author’s opinion, at the present time, there is no reliable criterion which allows the development of a symptomatic spinal instability to be predicted in patients with a preoperative spondylolisthesis and therefore fusion as a first line procedure is still debatable. [ABSTRACT FROM AUTHOR]

Summary Background : Somatostatin analogues are the best therapy for controlling the symptoms of malignant carcinoid syndrome. Octreotide acetate given as subcutaneous injection up to three times daily, intramuscular Lanreotide injection given once per 1–2 weeks and monthly intramuscular Sandostatin LAR have demonstrated similar efficacy in short-term studies. Aim : To assess the long-term effect of Sandostatin LAR on the management of patients with malignant carcinoid syndrome. Methods : This was a 3-year retrospective study. Twenty-seven patients were assessed with a median follow-up of 23 months. Thirteen patients were switched from subcutaneous octreotide and 14 patients were octreotide naive. All patients showed avid uptake on indium-111 octreotide imaging. Results : Ten of the 13 patients previously on subcutaneous octreotide and 13 of the 14 patients who were octreotide naive had good symptom control on Sandostatin LAR. Over the period of follow-up, many patients showed progression of their tumour and required additional therapies. Patients expressed a preference for monthly intramuscular Sandostatin LAR as opposed to daily subcutaneous injections of octreotide. Although Sandostatin LAR was difficult to administer in certain instances, overall it was well tolerated. Conclusions : Sandostatin LAR provides good long-term symptomatic control in patients with malignant carcinoid syndrome; it is well tolerated and patients expressed improved satisfaction in their management. [ABSTRACT FROM AUTHOR]

Background: Gallium-68 positron emission tomography (68Ga-PET) with the two registered somatostatin analogs, [68Ga]Ga-DOTA-Tyr3-octreotide ([68Ga]Ga-DOTA-TOC) and [68Ga]Ga-DOTA-Tyr3-octreotate ([68Ga]Ga-DOTA-TATE), where DOTA = 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid, is routinely used for imaging of somatostatin receptor (SST)-expressing tumors. We investigated copper-61 (61Cu) as an alternative radiometal for PET imaging of SST-expressing tumors. Compared to gallium-68, copper-61 (t1/2=3.33 h, Eß + max = 1.22 MeV) can be produced on a large scale, enables late time point imaging, and has the therapeutic twin copper-67. Herein, DOTA-TOC and 1,4, 7-triazacyclononane,1-glutaric acid-4,7-acetic acid (NODAGA)-TOC were labeled with copper-61 and compared with the clinically used [68Ga]Ga-DOTA-TOC. Methods: [61Cu]CuCl2 was produced from an irradiated natural nickel target. DOTA-TOC and NODAGA-TOC were labeled with [61Cu]CuCl2 in ammonium acetate buffer so to achieve a reaction pH of 5-6 and a temperature of 95°C for DOTA-TOC or room temperature for NODAGA-TOC. The radioligands were evaluated head-to-head in vitro using human embryonic kidney (HEK)-SST2 cells (affinity, binding sites, cellular uptake, and efflux) and in vivo using HEK-SST2 xenografts [PET/computed tomography (CT) imaging, biodistribution, and pharmacokinetics] and compared with [68Ga]Ga-DOTATOC, which was prepared using a standard procedure. Dosimetry estimates were made for [61Cu]Cu-NODAGA-TOC. Results: [61Cu]Cu-DOTA-TOC and [61Cu]Cu-NODAGA-TOC were prepared at an apparent molar activity of 25 MBq/nmol with radiochemical purities of ≥96% and ≥98%, respectively. In vitro, both presented a sub-nanomolar affinity for SST2 (IC50 = 0.23 and 0.34 nM, respectively). They were almost entirely internalized upon binding to SST2-expressing cells and had similar efflux rates at 37°C. In vivo, [61Cu]Cu-DOTA-TOC and [61Cu]Cu-NODAGATOC showed the same accumulation in SST2-expressing tumors. However, PET/CT images and biodistribution analyses clearly showed an unfavorable biodistribution for [61Cu]Cu-DOTA-TOC, characterized by accumulation in the liver and the abdomen. [61Cu]Cu-NODAGA-TOC displayed favorable biodistribution, comparable with [68Ga]Ga-DOTA-TOC at 1 h post-injection (p.i.). Notwithstanding, [61Cu]Cu-NODAGA-TOC showed advantages at 4 h p.i., due to the tumor retention and improved tumor-to-non-tumor ratios. The effective dose (2.41 × 10-3 mSv/MBq) of [61Cu]Cu-NODAGA-TOC, but also the dose to the other organs and the kidneys (9.65 × 10-2 mGy/MBq), suggested a favorable safety profile. Conclusion: Somatostatin receptor 61Cu-PET imaging not only matches the performance of 68Ga-PET at 1 h p.i. but has advantages in late-time imaging at 4 h p.i., as it provides improved tumor-to-non-tumor ratios. [61Cu]Cu-NODAGATOC is superior to [61Cu]Cu-DOTA-TOC in vivo. The use of the chelator NODAGA allows quantitative labeling with copper-61 at room temperature and enables the straightforward use of a kit formulation for simple manufacturing in medical centers. [ABSTRACT FROM AUTHOR]

Introduction: Faced to the growing development of collecting systematic molecular analyses in relapsed pediatric cancers to transform their targeted matched therapies, this study aimed to assess the clinical and therapeutic indications of systematic diagnostic genomic explorations performed in pediatric solid cancers to determine which type of screening and if it afford at relapse time an accurate targeted strategy. Methods: A total of 280 patients less than 22 years, referred at the University Hospitals of Strasbourg for a newly diagnosed solid tumor from January 2015 to December 2021, were prospectively genomically investigated since diagnosis. Using 7 different molecular tests going from single-gene methods (IHC, FISH, RT-PCR, Sanger sequencing, droplet digital PCR) to largescale analyses (Next-Generation sequencing, RNAsequencing and FoundationOne®CDx), we explored retrospectively the molecular findings in those pediatric solid tumors (except hematolymphoid cancers) to improve diagnosis, prognosis assessment and relapse therapeutics. Results: One hundred and ninety-eight patients (71%) underwent molecular biology (MB) at diagnosis. Thirty-eight different histologies were grouped into cerebral tumors (30%), sarcomas (26%, bone and soft tissues), various blastomas (27%), and other entities (17%). Over a median 40-month follow-up, the overall survival rate of patients was 85% and the relapse rate 28%. Of the 326 analyses carried out, 245 abnormalities (single nucleotide variations: 50%, fusions: 25%, copy number alteration: 20%) concerning 70 oncogenes were highlighted. The overall clinical impact rate was 84%. Broad-spectrum analyses had a higher therapeutic impact (57%) than the targeted analyses (28%). 75% of broad-spectrum tests found an actionable variant conducting 23% of patients to receive rapidly a matched targeted therapy since first relapse. Conclusion: Our experience highlighted the clinical utility of molecular profiling of solid tumors as soon as at diagnosis in children to expect improving access to innovative agents at relapse. [ABSTRACT FROM AUTHOR]

The incidence of gastroenteropancreatic neuroendocrine neoplasms (GEP NEN) is increasing at a rapid pace and is becoming an increasingly important consideration in clinical care. Epidemiological data from multiple countries indicate that the incidence of gastroenteropancreatic neuroendocrine neoplasms (GEP NEN) exhibits regional, site-specific, and gender-based variations. While the genetics and pathogenesis of some GEP NEN, particularly pancreatic NENs, have been investigated, there are still many mechanisms that require further investigation. The management of GEP NEN is diverse, but surgery remains the primary option for most cases. Peptide receptor radionuclide therapy (PRRT) is an effective treatment, and several clinical trials are exploring the potential of immunotherapy and targeted therapy, as well as combination therapy. [ABSTRACT FROM AUTHOR]

Liposarcoma (LPS) is the second most common kind of soft tissue sarcoma, and a heterogeneous malignant tumor derived from adipose tissue. Up to now, the prognostic value of BAG1 or BAG2 in LPS has not been defined yet. Expression profiling data of LPS patients were collected from TCGA and GEO database. Survival curves were plotted to verify the outcome differences of patients based on BAG1 or BAG2 expression. Univariate and multivariate Cox regression models were used to analyze the prognostic ability of BAG1 or BAG2. Chaperone's regulators BAG1 and BAG2 were identified as prognostic biomarkers for LPS patients, which exhibited distinct expression patterns and survival outcome prediction performances. Patients with high BAG2 expression and/or low BAG1 expression had worse prognosis. Enrichment analysis showed that BAG1 was involved in negative regulation of TGF-β signaling. Low expression of BAG1 was associated with high abundance of regulatory T cells (Tregs). The 2-gene signature model further confirmed the improved risk assessment performance of BAG1 and BAG2: high risk patients displayed poor prognosis. BAG1 and BAG2 are supposed to be potential prognostic biomarkers for LPS and have impacts on liposarcomagenesis and immune infiltration in distinctive manners, which may function as potential therapy targets (BAG1 agonists/BAG2 inhibitors) for LPS. [ABSTRACT FROM AUTHOR]

Many fundamental concepts of synoptic-scale extratropical dynamics are based on the quasi-geostrophic equations of a dry atmosphere. This "dry dynamics" provides the essential understanding of, for example, the formation of extratropical cyclones and the propagation of Rossby waves and makes potential vorticity (PV) a materially conserved quantity. Classically, for extratropical weather systems, the importance of so-called "diabatic effects", e.g. surface fluxes, phase changes of water in clouds, and radiation, has been regarded as secondary compared to the dry dynamical processes. As outlined in this review article, research during recent decades has modified this view of the role of diabatic processes. A combination of complementary research approaches revealed that the nonlinear dynamics of extratropical cyclones and upper-tropospheric Rossby waves is affected – in some cases strongly – by diabatic processes. Despite the violation of material PV conservation in the presence of diabatic processes, the concept of PV has been of utmost importance to identify and quantify the role of diabatic processes and to integrate their effects into the classical understanding based on dry dynamics. This review first summarises the theoretical concepts of diabatic PV modification, moist PV, and slantwise moist convection and provides a concise overview of early research on diabatic effects until the late 1970s. Two poorly predicted high-impact cyclones affecting eastern North America then triggered an impressive diversity of efforts to investigate the role of diabatic processes in rapid cyclone intensification in the last 2 decades of the 20th century. These research activities, including the development of sophisticated diagnostics, growing applications of the Lagrangian perspective, real-case and idealised numerical experiments, and dedicated field experiments, are reviewed in detail. This historical perspective provides insight about how societal relevance, international collaboration, technical development, and creative science contributed to establishing this important theme of dynamical meteorology. The second part of the review then more selectively outlines important achievements in the last 2 decades in our understanding of how diabatic effects, in particular those related to cloud microphysics, affect the structure, dynamics, and predictability of different types of extratropical cyclones and their mesoscale substructures, upper-tropospheric blocks, Rossby waves, and interactions. A novel aspect is the relevance of research on diabatic processes for climate change research. The review closes by highlighting important implications of investigating diabatic processes in extratropical weather systems for the broader field of weather and climate dynamics and its fundamentals and representation in numerical models. [ABSTRACT FROM AUTHOR]