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The Bohai Sea is the receiving basin of the Yellow River (Huanghe), one of the largest contributors of global terrestrial materials to the sea. An area of fine-grained sediment deposition extends northeast of the Yellow River and its formation mechanism is still unclear. A calibrated, high-resolution coupled model of the Bohai and Yellow Seas was used to investigate sediment transport pathways in the region under the influence of strong and varying winds in winter. Numerical results highlight the importance of storm events in modifying the regional sediment resuspension and transport. During winter, the prevailing winds are typically from the northeast and northwest, interspersed by relaxation periods with weak winds. Winds influenced the currents and sediment movement in the Bohai Sea through several processes: local wind waves and resuspension, coastal-trapped waves in the Bohai Sea, and remotely forced coastal-trapped waves in the Yellow Sea. During northwesterly winds, sediment off the Yellow River Delta was mainly transported eastward along the south coast of Bohai Sea and escaped the Bohai Sea through the southern Bohai Strait. When northeasterly winds prevailed, sediment transport split into three branches: westward into the Bohai Bay, northeastward to the central Bohai Sea, and eastward along the south coast of Bohai Sea. Modeling results driven by synoptic winds indicate a more complex sediment transport pattern than previously understood with monthly average wind forcing. These findings challenge the traditional view of predominantly eastward sediment transport during winter and help to explain the observed grain size distribution in the Bohai Sea, in particular the accumulation of fine-grained sediments northeast of the Yellow River. • Storms are important in modifying the regional sediment resuspension and transport. • Sediment movement in BS depends on local winds, its related CTWs and CTWs in YS. • The formation of muddy areas northeast of the YRD is related to the varied winds. [ABSTRACT FROM AUTHOR]

The South China Sea (SCS) is a highly semi-enclosed marginal sea located in the East Asian monsoon region. This paper proposes interesting aspects of the unique feature of the SCS waves in response to tropical cyclone's passage when large-scale winter monsoon winds prevail. We use the wave model WaveWatch III to study the wave characteristics of typhoon Durian (2006) passing over the middle of SCS in early December 2006, and state the new understanding acquired in the aspects of the tropical cyclone generated waves in the SCS during winter monsoon surge. In light of this, the role of the large-scale NE monsoon winds on winter typhoon wave field characteristics in the SCS are highlighted by conducting sensitivity experiments with and without the NE monsoon winds. The NE monsoon winds weakly affect the SWH field near the typhoon track and strongly away from the track, especially in the deep water area of the northern SCS where the NE monsoon winds produce high waves. Comparisons between the two experiments show the effect of the NE monsoon winds on the directional wave spectra in the SCS, suggesting that the monsoon-generated swells do not decay and remain throughout the typhoon period. [ABSTRACT FROM AUTHOR]

Highlights • Artificial inoculation of onion bulbs causes basal rot. • Pathogenic infection increases emission of volatile organic compounds. • Volatile organic compounds correlate positively with the extent of infection. • 1-Propanethiol, methyl propyl sulfide and styrene are markers of fungal infection. Abstract Diseases develop during the storage of onions. To minimize losses, new methods are needed to identify diseased bulbs early in storage. Volatile organic compounds (VOCs), the respiration rate, weight loss, and the dry matter content were investigated for 1–7 weeks post inoculation of bulbs with water (control) and two strains (Fox006 or Fox260) of Fusarium oxysporum f. sp. cepae. Photos, multispectral image analysis, and real-time polymerase chain reaction (PCR) showed no infection in the control onions, weak pathogenic infection in Fox006-onions, and strong pathogenic infection in Fox260-onions at week 7 post inoculation. Infected bulbs exhibited increased respiration rate, increased VOC emission rate, and increased weight loss. The control and Fox006-onions did not respond to inoculation and had similar reaction pattern. Forty-three different VOCs were measured, of which 17 compounds had sulfur in their chemical structure. 1-Propanethiol, methyl propyl sulfide, and styrene were emitted in high concentrations and were positively correlated with the extent of infection (r = 0.82 – 0.89). Therefore, these compounds were the most promising volatile markers of Fusarium basal rot infection. For the first time, we show that the extent of fungal infection determined by real-time PCR in onion bulbs is related with VOC emission. [ABSTRACT FROM AUTHOR]

A calibrated, coupled model of the Bohai and Yellow Seas is developed to investigate sediment transport and deposition processes around a distinct clinoform east of the Shandong Peninsula. On the clinoform, deposition patterns vary seasonally with the regional hydrography and the East Asian Monsoon. A local maximum in sediment deposition is located offshore from spring to autumn, whereas sediment deposition is greater near the coast and decreases seaward in winter. From spring to autumn, a thermal front develops around the Yellow Sea Cold Water Mass and limits sediment transport seaward, enhancing sediment deposition and leading to high deposition rate in a focused region offshore. Sediment is transported to the region by the southward basin-scale cyclonic circulation, and the cross-shelf gradient in stratification, sediment resuspension, and vertical mixing at the front lead to a convergence in sediment transport and deposition. Sediment deposition over the clinoform is the greatest during the transition from summer to autumn when the thermal front persists but sediment supply increases. This results from weakened stratification and greater resuspension near the coast due to stronger winds, waves, and sea-surface cooling. A seasonal seaward shift of the frontal position toward deeper water is consistent with the decreasing heat flux, and the corresponding shift in location of maximum sediment deposition shifts seaward. In winter, the thermocline disappears and vertical mixing increases throughout the region. SSC over the clinoform increases due to active resuspension and net deposition decreases, and instead sediment is transported seaward and deposits in the South Yellow Sea. Current velocity, SSC and sediment deposition rates decrease seaward, in contrast to the focused trapping at the thermal front from spring to autumn. The deposition rates around the thermal front from spring to autumn are greater than in the winter, so the high net sediment accumulation over an annual cycle occurs in the same region as the longer-term accumulation preserved in the omega-shaped clinoform. • Sediment deposition rates are increased at a thermal front from spring to autumn at east of the Shandong Peninsula. • Seasonal shifts of the position of thermal front and sediment depo-center broaden the sediment accumulation region. • The monsoonal climate plays a dominant role in the seasonally varying circulation and sediment transport in the Yellow Sea. [ABSTRACT FROM AUTHOR]

A long shelf life of onions (Allium cepa L.) is of high importance in the onion industry. Onions are dried and stored in large wooden boxes that are difficult to access. Monitoring temperature and relative humidity during these processes is challenging. Moreover, quality may change in storage without being noticed. Therefore, there is a need to find alternative methods for monitoring and controlling the drying and storage processes of onions and to identify early changes in quality during storage. The potential use of online measurements of temperature and relative humidity (RH) in the vicinity of onions was evaluated during drying and long-term storage of six onion batches (four cultivars and three selections of one of the cultivars) in commercial storage. The batches varied in bulb weight, dry matter content, firmness and disease incidence. The dry matter content and firmness decreased during storage, while the respiration rate and incidences of individual and total disease increased. Two of the batches had low storability with high disease incidences and high average temperatures and variations in the RH. The results showed that tracking the temperature and RH in the vicinity of the onions is a promising tool for improving the drying and storage processes in commercial storage and for identifying onion batches with reduced storability early in storage. [ABSTRACT FROM AUTHOR]

Abstract Basal rot is a common onion disease and is mainly caused by Fusarium oxysporum f. sp. cepae and Fusarium proliferatum. To study the possibility of using volatile organic compounds (VOCs) as biomarkers for these fungi, pathogenic isolates of F. oxysporum and F. proliferatum from onions were cultivated in onion medium and VOCs were measured by solid phase microextraction (SPME). Forty-two compounds were detected, and thirty of these compounds were highly related to fungal metabolic activity. Allyl mercaptan was specific to F. oxysporum isolate Fox006. Analysis of the VOCs showed significant differences between the two species and among different isolates within the same species. Sixteen of the VOCs showed were highly positively correlated with the fungal biomass estimated by real-time polymerase chain reaction (PCR). Ethanol, ethyl formate, ethyl acetate, 2-methyl-1-propanol, methyl thioacetate, n-propyl acetate and 3-methyl-1-butanol are volatile metabolites that were potential indicators of Fusarium growth on onions. Highlights • VOCs from onion-pathogenic Fusarium fungi were studied for the first time. • Specific VOCs were detected from F. oxysporum and F. proliferatum. • Fungal volatiles were quantitatively related with fungal biomass. • VOCs from F. oxysporum isolates correlated with their taxonomics. [ABSTRACT FROM AUTHOR]

This study investigates the deep circulation in the South China Sea (SCS) using oceanographic observations combined with results from a bottom layer reduced gravity model. The SCS water, 2000 m below the surface, is quite different from that in the adjacent Pacific Ocean, and it is characterized by its low dissolved oxygen (DO), high temperature and low salinity. The horizontal distribution of deep water properties indicates a basin-scale cyclonic circulation driven by the Luzon overflow. The results of the bottom layer reduced gravity model are consistent with the existence of the cyclonic circulation in the deep SCS. The circulation is stronger at the northern/western boundary. After overflowing the sill of the Luzon Strait, the deep water moves broadly southwestward, constrained by the 3500 m isobath. The broadening of the southward flow is induced by the downwelling velocity in the interior of the deep basin. The main deep circulation bifurcates into two branches after the Zhongsha Islands. The southward branch continues flowing along the 3500 m isobath, and the eastward branch forms the sub-basin scale cyclonic circulation around the seamounts in the central deep SCS. The returning flow along the east boundary is fairly weak. The numerical experiments of the bottom layer reduced gravity model reveal the important roles of topography, bottom friction, and the upwelling/downwelling pattern in controlling the spatial structure, particularly the strong, deep western boundary current. [ABSTRACT FROM AUTHOR]

Puerarin is an isoflavonoid isolated from the Chinese herb, Kudzu roots (also known as Gegen), which has been widely used for the treatment of hypertensive diseases and diabetic mellitus in traditional Chinese medicine. Dahl salt-sensitive (DS) rat is a genetic model of salt-sensitive hypertension with cardiovascular injury and vascular insulin resistance. Here, we investigated whether puerarin improved vascular insulin resistance and attenuated cardiac and aortic remodeling in salt-sensitive hypertension. DS rats were given a normal (NS) or high salt diet (HS) for five weeks. An additional group of DS rats was pretreated with puerarin and NS for 10 days, then switched to HS plus puerarin for five weeks. HS for five weeks increased systolic blood pressure (SBP), cardiac hypertrophy and fibrosis, and aortic hypertrophy with increased the expression of phosphor-ERK1/2 in the aorta and heart; puerarin attenuated cardiac and aortic hypertrophy, cardiac fibrosis and phosphor-ERK1/2 with a mild reduction in SBP. Hypertensive rats also manifested impairment of acetylcholine- and insulin-mediated vasorelaxation and insulin-mediated Akt and eNOS phosphorylation associated with the activation of NFB/TNF/JNK pathway. Puerarin improved acetylcholine- and insulin-mediated vasorelaxation and insulin-stimulated Akt/NO signaling with the inhibition of the NFB inflammatory pathway. Our results demonstrated that in salt-sensitive hypertension, puerarin improved vascular insulin action with cardiovascular beneficial effects. Our results found that the underlying mechanisms may involve its inhibition of NFB/JNK and ERK1/2 pathway. These results suggest that puerarin could be used as a new antihypertensive agent to expand our armamentarium for the prevention and treatment of end-organ damage in individuals with hypertension and metabolic diseases. [ABSTRACT FROM AUTHOR]

A calibrated, coupled model (COAWST) of the Bohai and Yellow Seas was employed to examine the contributions of waves and tides to the sediment transport, and to understand the dynamic factors controlling bottom sediment resuspension around the Shandong Peninsula. The numerical results, which were consistent with the in-situ observations, illustrated two events with large values of suspended sediment concentration (SSC). The two events had similar wave heights, but SSC during the second event was more than twice that of the first one. Numerical experiments indicated that the large values of SSC east of the Shandong Peninsula were mainly ascribed to local resuspension, and that waves played a primary role in sediment resuspension for both high SSC events. The differences in SSC and bottom shear stress between the two events were mainly due to differences in wave period, representing about 65% of the total difference, and the non-linear interactions of wave and currents played a secondary role. Therefore, wave period is an important factor along with wave amplitude in the contribution of waves to sediment resuspension in this region. The combined actions of waves and currents dynamics dominate the sediment resuspension rather than either factor in isolation. Due to sheltering effect by the Shandong Peninsula, the wave effect on sediment resuspension was less to the south of the Peninsula than that to the north and east. In addition, the Shandong Coastal Current was enhanced by the northerly wind leading to more sediment transport southward along the coast of Shandong Peninsula, but this has small effect on the difference of suspended sediment concentration between two storm events. • Large values of suspended sediment concentration east of the Shandong Peninsula is mainly due to local resuspension. • The combined actions of waves and tidal dynamics dominate the sediment resuspension. • Wave period is an important factor along with wave amplitude in the contribution of waves to sediment resuspension. [ABSTRACT FROM AUTHOR]

Wang, A. M.; Wang, H. J.; Bi, N. S., and Wu, X., 2016. Sediment transport and dispersal pattern from the Bohai Sea to the Yellow Sea. Suspended sediment flux, dispersal patterns and its possible mechanisms in the Bohai Sea were investigated based on the observational data in May and November, 2012, data retrieved from MODIS imageries and the ocean current data from HYCOM. The sediment dispersal pattern and the annual net sediment flux presented evident seasonal variability. During wintertime, the prevailing strong northerly winds and the related high wave heights resuspended the sediment along the coast of the Huanghe Delta that was transported into the Laizhou Bay along its western coast and exported to the Bohai Strait through the eastern Laizhou Bay as enhanced by the coastal current. The wintertime contributed approximately 4.84 Mt of suspended sediment flux to the Yellow Sea, as higher than the flux in summertime by one order. In summertime, the less energetic environment together with the stratified water column was unfavorable to the sediment export to the Yellow Sea. As a result, the sediment delivered by the Huanghe to the sea in summertime mostly accumulated within the subaqueous delta, which acted as a primary source of sediment export to the Yellow Sea in wintertime. [ABSTRACT FROM AUTHOR]

Distribution of suspended sediment in the Bohai Sea and its seasonal variability were investigated based on the datasets obtained from four cruises carried out in different seasons in 2010 and 2012. The results indicate that the spatial distribution of suspended sediment in the Bohai Sea was dominated by the river input and coastal resuspension that depends on the type of local surface sediment and hydrodynamics. The suspended sediment was mostly concentrated in the southern Bohai Sea particularly around the Yellow River Delta in the summer season as impacted by the river plume but confined within a very limited area near the river mouth, whereas in winter–spring seasons sediment concentration became much higher as a result of active coastal resuspension induced by energetic wave actions in the shallow water. High sediment concentration was found in Liaodong Bay in winter–spring seasons when dynamics become strong but decrease significantly in summer seasons. The sediment concentration in the southern Bohai Strait was much higher than that in the northern part, suggesting evident sediment export to the Yellow Sea particularly in the winter–spring seasons. Strong seasonal variability of suspended sediment distribution in the Bohai Sea was consistent with the monsoon activity and associated wave actions and coastal currents that are varying seasonally. The dominance of seasonal monsoon signal and associated wave dynamics make the subaqueous Yellow River delta to be a major sink for the terrestrial sediment in the summer seasons, but transiting to a primary source in winter–spring seasons for sediment redistribution in the Bohai Sea and sediment export to the Yellow Sea. The seasonal patterns of suspended sediment distribution in the Bohai Sea and the dominance of monsoon activities may provide a good reference to understanding the sediment transport in the China Shelf Seas where sediment resuspension and coastal circulation are evidently driven by monsoon. [ABSTRACT FROM AUTHOR]

Highlights: [•] The d-galactose-6-sulfurylase was able to remove the sulfate from precursor carrageenan to form κ-carrageenan. [•] The existence of μ-carrageenan was detected in Betaphycus gelatinus by FTIR. [•] The d-galactose-6-sulfurylase was purified to monomeric protein which was metalloid and sensitive to pH and temperature. [Copyright &y& Elsevier]

• Fe was highly dispersed on MIL-100(Fe) derived porous carbon with a high loading (>37 wt%). • XRD and HRTEM show particle size decrease for Na, Cu, and Ru-promoted catalysts. • Cu-promoted catalysts show a high CO conversion and a stable FT activity. • Na addition increased O/P ratio and C 5+ selectivity in product distribution. • Ru-promoted catalysts show a deactivation at high reaction temperature (340 °C). Fischer-Tropsch synthesis (FTS) is studied over Metal-organic framework (MOF) based porous carbon supported iron catalysts promoted with Na, Cu and Ru metals. The FTS results showed that all promoters can enhance CO conversion, FT product yield (FTY), and CO 2 selectivity is also increased. Among all promoters, Cu-based sample exhibits the best promoting effect in activity (FTY=0.154 mol CO g Fe −1 h −1). Interestingly, the activity is about 2 times higher to that of unpromoted catalysts. Ru-contained sample exhibits the most significant influence on the light hydrocarbon production, with the C 1–4 hydrocarbon selectivity above 70%. Na-contained sample performed the highest C 5+ selectivity of 66% with a medium CO conversion of 51%. The improved FTS activity and selectivity of promoted catalysts is related to the decrease in iron particle size as confirmed by X-ray diffraction (XRD), and transmission electron microscopy (TEM). It is confirmed, the small iron particles of the promoted catalyst are stabilized on the surface of carbon matrix, preventing further aggregation at high temperature, which is contributed to the improved FTS performance. Our research study showed that Na, Cu and Ru can not only affect the distribution of resulting products but also improve the activity of FTS. In short, the effect of Cu is remarkable on the MOF-derived catalyst. Similarly, the MOF-derived porous carbon catalysts confined the iron species during the reaction process, provide a clear structure-activity relationship between the promoters and the catalytic performance. Finally, this novel work can provide a new pathway for metal-organic framework-derived Fe-embedded porous carbon catalysts for Fischer-Tropsch synthesis. [Display omitted] [ABSTRACT FROM AUTHOR]

Colorectal cancer (CRC) is a common malignant tumor that primarily affects the elderly population. Surgery is one of the main treatment modalities for CRC. Frailty is a prevalent characteristic among the elderly and a leading cause of mortality. The frailty index (FI) is a comprehensive tool for assessing patients' frailty status, quantifying indicators such as weight loss, fatigue, and nutritional status, to reflect the degree of frailty. In recent years, the FI has undergone modifications to more accurately evaluate the risk of surgical complications and prognosis in CRC patients. This review summarizes the methods for frailty assessment, the development and modifications of the FI, and compiles the research findings and applications of the FI in predicting surgical complications, postoperative recovery, and survival rates in CRC patients. Furthermore, limitations in the current modified frailty index (mFI) and future research directions are discussed. This review provides essential references for further understanding the role of frailty in CRC patients and the clinical application of the mFI. [ABSTRACT FROM AUTHOR]

Pulmonary vascular remodeling is the most important pathological characteristic of pulmonary arterial hypertension (PAH). No effective treatment for PAH is currently available because the mechanism underlying vascular remodeling is not completely clear. CD248, also known as endosialin, is a transmembrane protein that is highly expressed in pericytes and fibroblasts. Here, we evaluated the role of CD248 in pulmonary vascular remodeling and the processes of PAH pathogenesis. Activation of CD248 in pulmonary artery smooth muscle cells (PASMCs) was found to be proportional to the severity of PAH. CD248 contributed to platelet‐derived growth factor‐BB (PDGF‐BB)‐induced PASMC proliferation and migration along with the shift to more synthetic phenotypes. In contrast, treatment with Cd248 siRNA or the anti‐CD248 therapeutic antibody (ontuxizumab) significantly inhibited the PDGF signaling pathway, obstructed NF‐κB p65‐mediated transcription of Nox4, and decreased reactive oxygen species production induced by PDGF‐BB in PAMSCs. In addition, knockdown of CD248 alleviated pulmonary vascular remodeling in rat PAH models. This study provides novel insights into the dysfunction of PASMCs leading to pulmonary vascular remodeling, and provides evidence for anti‐remodeling treatment for PAH via the immediate targeting of CD248. [ABSTRACT FROM AUTHOR]

Based on coastal tide level, satellite altimetry, and sea surface temperature (SST) data of offshore areas of China’s coast and the equatorial Pacific Ocean, the regional characteristics of the effects of the El Niño-Southern Oscillation (ENSO) on the sea level in the China Sea were investigated. Singular value decomposition results show a significant teleconnection between the sea level in the China Sea and the SST of the tropical Pacific Ocean; the correlation coefficient decreases from south to north. Data from tide gauges along China’s coast show that the seasonal sea-level variations are significantly correlated with the ENSO. In addition, China’s coast was divided into three regions based on distinctive regional characteristics. Results obtained show that the annual amplitude of sea level was low during El Niño developing years, and especially so during the El Niño year. The ENSO intensity determined the response intensity of the annual amplitude of the sea level. The response region (amplitude) was relatively large for strong ENSO intensities. Significant oscillation periods at a timescale of 4-7 years existed in the sea level of the three regions. The largest amplitude of oscillation was 1.5 cm, which was the fluctuation with the 7-year period in the South China Sea. The largest amplitude of oscillation in the East China Sea was about 1.3 cm. The amplitude of oscillation with the 6-year period in the Bohai Sea and Yellow Sea was the smallest (less than 1 cm). [ABSTRACT FROM AUTHOR]

The alkylation of phenol with methanol was studied in vapor phase over 5 wt% cerium supported on HZSM-5 catalyst prepared by three different methods, such as impregnation, ball mixing and solid-state ion-exchange. The catalytic activities are tested in a fixed-bed reactor, and the physicochemical properties of the catalyst are characterized with X-ray powder diffraction, scanning electron microscope, nitrogen physical sorption, Fourier Transform Infrared Spectrometer, Infrared spectra of pyridine adsorption, X-ray photoelectron spectroscopy and temperature-programmed desorption of ammonia. It was found that the dispersion form of cerium was greatly affected by the preparation method, and difference in acidity affects catalytic performance in the alkylation reaction of phenol and methanol. Ball milling method generated greater amount of strong Brønsted acidic sites and lower specific surface area than other catalysts, which led to higher carbon deposition rate and lower catalyst activity. The catalyst prepared by impregnation method yields higher Lewis acid concentration (2.09 μmol/g) and more chemisorbed oxygen due to the increased Ce3+ concentration in the lattice, resulted in an improved o-cresol selectivity of 48%. Furthermore, the catalyst prepared by solid-phase ion-exchange method produces a catalyst with appropriate B/L ratio of 60.6 and large specific surface area of 389.0 m2/g, which yields the highest phenol conversion of 23%. [ABSTRACT FROM AUTHOR]

Mud depocenters play an important role in the sediment source–sink system of marginal seas; however, the factors controlling the formation of mud deposits are not well understood. This study focused on Core LHSD-1, which is located on the southern margin of the Shandong Peninsula mud wedge in the northwestern part of the South Yellow Sea. Elemental ratios, such as the C/S ratio (the mass ratio of total organic carbon and total sulfur) and Sr/Ba ratio, were used to track the changes in paleosalinity during the late Quaternary and to investigate the mechanisms that influenced the formation of the mud wedge off the Shandong Peninsula. Our results indicate that Core LHSD-1 likely recorded two transgression events, corresponding to MIS3 and MIS1. Following sea-level rise after the Last Glacial Maximum (LGM), transgressive deposition began in the study area at approximately 11.5 kyr BP. During the early Holocene, fluctuating water salinity was recorded by Sr/Ba ratios, which became stable at approximately 6.8 kyr BP when the sea level reached its highstand. At the same time, the modern current system was established, and a significant amount of terrestrial material, such as refractory organic carbon and iron, has been transported to the study area by enhanced coastal currents, which was documented by elevated C/S ratios (C/S > 2.8) in mud sediments. Our findings indicate that marine transgressions controlled the initial development of the mud depocenter and that the establishment of the modern ocean current system promoted the rapid deposition of mud sediments, which corresponded to the growth of global mud depocenters. • C/S and Sr/Ba ratios indicate two transgressions corresponding to MIS1 & MIS3. • Mud sediments were initially deposited at 11.5 ka due to the deglacial sea-level rise. • Paleosalinity records indicate the mud depocenter was mainly formed after 6.8 ka. • High C/S ratios since 6.8 ka indicate the establishment of the modern current system. [ABSTRACT FROM AUTHOR]

A novel and simple process for the preparative extraction, separation, and purification of high-purity (–)-borneol and xanthoxylin, synchronously, from the leaves ofBlumea balsamifera(L.) DC was developed. Simultaneous distillation and extraction, silica gel column chromatography, and recrystallization were applied to the preparation. Two objective compounds with the purity of 98% were obtained, and the losses were minimal during the whole procedure. It is the first time that the preparation of high-purity (–)-borneol and xanthoxylin at the same time were achieved. [ABSTRACT FROM AUTHOR]

The present study elucidates the underlying mechanism through which PUE confers protection against CDDP-induced ototoxicity. PUE effectively regulates Calcium overload and suppresses the production of Reactive Oxygen Species (ROS) through the regulation of TRPV1 expression, thereby mitigating damage to the auditory system. To illustrate this mechanism, a schematic diagram has been presented. [Display omitted] Puerarin (PUE), a flavonoid derivative with vasodilatory effects found in the traditional Chinese medicine kudzu, has anti-sensorineural hearing loss properties. However, the mechanism of its protective effect against ototoxicity is not well understood. In this study, we used in vitro and in vivo methods to investigate the protective mechanism of puerarin against cisplatin (CDDP)-induced ototoxicity. We established an ototoxicity model of CDDP in BALB/c mice and assessed the degree of hearing loss and cochlear cell damage. We used bioinformatics analysis, molecular docking, histological analysis, and biochemical and molecular biology to detect the expression of relevant factors. Our results show that puerarin improved CDDP-induced hearing loss and reduced hair cell loss. It also blocked CDDP-induced activation of TRPV1 and inhibited activation of IP3R1 to prevent intracellular calcium overload. Additionally, puerarin blocked CDDP-stimulated p65 activation, reduced excessive ROS production, and alleviated cochlear cell apoptosis. Our study provides new evidence and potential targets for the protective effect of puerarin against drug-induced hearing loss. Puerarin ameliorates cisplatin-induced ototoxicity and blocks cellular apoptosis by inhibiting CDDP activated TRPV1/IP3R1/p65 pathway, blocking induction of calcium overload and excessive ROS expression. [ABSTRACT FROM AUTHOR]

Obesity and cigarette smoke are major cardiovascular (CV) risk factors and, when coexisting in the same individuals, have additive/synergistic effects upon CVD. We studied the mechanisms involved in nicotine enhancement of CVD in Sprague Dawley rats with diet–induced obesity. The rats were fed either a high fat (HFD) or normal rat chow diet with or without nicotine (100 mg/L in drinking water) for 20 weeks. HFD rats developed central obesity, increased systolic blood pressure (SBP), aortic superoxide (O2-) production, and impaired endothelial nitric oxide synthase (eNOS) and endothelium-dependent relaxation to acetylcholine (EDR). Nicotine further increased SBP, O2- and impaired eNOS and EDR in obese rats. In the peritoneal macrophages from obese rats, tumor necrosis factor (TNF) α, interleukin 1β and CD36 were increased, and were further increased in nicotine-treated obese rats. Using PCR array we found that 3 of 84 target proinflammatory genes were increased by 2–4 fold in the aorta of obese rats, 11 of the target genes were further increased in nicotine-treated obese rats. HUVECs, incubated with conditioned medium from the peritoneal macrophages of nicotine treated-obese rats, exhibited reduced eNOS and increased NADPH oxidase subunits gp91phox and p22phox expression. Those effects were partially prevented by adding anti-TNFα antibody to the conditioned medium. Our results suggest that nicotine aggravates the CV effects of diet–induced obesity including the oxidative stress, vascular inflammation and endothelial dysfunction. The underlying mechanisms may involve in targeting endothelium by enhancement of macrophage-derived TNFα. [ABSTRACT FROM AUTHOR]

Oxidative stress is considered to be a major contributor to age-related hearing loss (ARHL). Here, we investigated whether pomegranate peel extract (PPE) protected against hearing loss by decreased oxidative stress in the cochlea of D -galactose–induced accelerated aging mice. The aging mice exhibited an increase in hearing threshold shifts and hair cells loss, which were improved in the PPE-treated aging mice. The aging mice also exhibited an increase in 4-hydroxynonenal, the expression of protein phosphatase 1 nuclear targeting subunit (PNUTS), p53 and caspase-3, and a decrease in protein phosphatase 1 (PP1) and MDM2 in the cochlea. PPE treatment reversed the changes in aforementioned molecules. Our results suggested that PPE can protect against ARHL, the underlying mechanisms may involve in the inhibition of oxidative damage of cochlea, possibly by regulating PNUTS/PP1 pathway. The results from the present study provide a new therapeutic strategy to use PPE for prevention of ARHL. [ABSTRACT FROM AUTHOR]

The water-sediment regulation scheme (WSRS), beginning in 2002, is an unprecedented engineering effort to manage the Yellow River with the aims to mitigate the siltation both in the lower river channel and within the Xiaolangdi Reservoir utilizing the dam-regulated flood water. Ten years after its initial implementation, multi-disciplinary indicators allow us to offer a comprehensive review of this human intervention on a river-coastal system. The WSRS generally achieved its objective, including bed erosion in the lower reaches with increasing capacity for flood discharge and the mitigation of reservoir siltation. However, the WSRS presented unexpected disturbances on the delta and coastal system. Increasing grain size of suspended sediment and decreasing suspended sediment concentration at the river mouth resulted in a regime shift of sediment transport patterns that enhanced the disequilibrium of the delta. The WSRS induced an impulse delivery of nutrients and pollutants within a short period (~ 20 days), which together with the altered hydrological cycle, impacted the estuarine and coastal ecosystem. We expect that the sediment yield from the loess region in the future will decrease due to soil-conservation practices, and the lower channel erosion will also decrease as the riverbed armors with coarser sediment. These, in combination with uncertain water discharge concomitant with climate change, increasing water demands and delta subsidence, will put the delta and coastal ocean at high environmental risks. In the context of global change, this work depicts a scenario of human impacts in the river basin that were transferred along the hydrological pathway to the coastal system and remotely transformed the different components of coastal environment. The synthesis review of the WSRS indicates that an integrated management of the river-coast continuum is crucially important for the sustainability of the entire river-delta system. The lessons learned from the WSRS in the Yellow River provide insights to the integrated management of large rivers worldwide. [ABSTRACT FROM AUTHOR]

Puerarin, a major isoflavonoid compound from Chinese herb Kudzu roots, has been widely used for the treatment of hypertensive and cardiovascular diseases in China. Here, we investigated puerarin’s beneficial effects on the cardiovascular system in angiotensin (Ang) II-induced hypertensive rats. Sprague–Dawley rats were treated with Ang II for 5 days or with puerarin for 10 days followed by Ang II and puerarin for 5 days. Endothelium-dependent relaxation (EDR) to acetylcholine was determined using an organ chamber bath. Ang II increased the systolic blood pressure (SBP: 178 ± 5 mmHg vs. 112 ± 3 mmHg in control,p< 0.05), aortic (30%,p< 0.05), and left ventricular (LV) weight (23%); puerarin reduced SBP (160 ± 2 mmHg,p< 0.05), aortic, and left ventricular weight in Ang II-infused rats. Puerarin also reduced aortic medial thickness and myocardial cell surface area in Ang II-infused rats. Compared with control rats, Ang II infused rats exhibited an impaired EDR with reduction in the protein expression of phosphor-eNOS at Ser 1177 and an increase in the expression of gp91phox (85%), p22phox (113%), transforming growth factor β1 (145%) and vascular cell adhesion molecule 1 (82%). Puerarin improved EDR and reversed the changes in Ang II-induced protein expression of above molecules. Our results demonstrate that in Ang II-induced hypertensive rats, puerarin protects against endothelial dysfunction and end organ damage with a mild reduction in SBP, and that the cardiovascular beneficial effects of puerarin may be in part attributed to its anti-oxidant and upregulation of phosphor-eNOS. [ABSTRACT FROM AUTHOR]

The temporal variations of sea surface chlorophyll- a concentration off the Yellow River Mouth were investigated using SeaWiFS and MODIS data over the period of 1997–2012. And the complex interplay of different dominant factors (e.g. river inputs and coastal dynamics) on the phytoplankton dynamics in different seasons was discussed. High concentration of nutrients from the Yellow River inputs plays a significant role in sustaining phytoplankton growth. Approximately 59% of the interannual variations of the chlorophyll- a concentration off the Yellow River Mouth were attributed to river inputs (water discharge, sediment load and median grain size of sediment particles). On seasonal time scale, the Yellow River discharge of water and nutrients is a major factor that influences the increased chlorophyll- a concentration from August to October (summer and autumn). From February to May, the stronger winds and enhanced wave actions resuspend and vertically mix the previously deposited riverine sediment and the associated nutrient, thus possibly contributing to higher chlorophyll- a concentration. [ABSTRACT FROM AUTHOR]

Abstract: Scorpion venoms metalloproteinase is involved in a number of important biological, physiological and pathophysiological processes. In this work, a complete sequence of metalloproteinase was first obtained from venom of scorpion Buthus martensi and named as BumaMPs1. BumaMPs1 has 393 amino acid residues containing with a molecular mass of 44.53 kDa, showing an isoelectric point of 5.66. The primary sequence analysis indicated that the BumaMPs1 contains a zinc-binding motif (HELGHNLGISH), methionine-turn motif (YIM), disintegrin-like domain (ETCD) and N-glycosylation site. The multiple alignment of its deduced amino acid sequence and those of other metalloproteinase showed a high structural similarly, mainly among class reprolysin proteases. The phylogenetic analysis showed early divergence and independent evolution of BumaMPs1 from other metalloproteinase. [Copyright &y& Elsevier]

Highlights: [•] The Gal-6-sulfurylase from Eucheuma striatum was first purified to homogeneity. [•] Enzymatic treatment improved the gel strength of κ-carrageenan to 1630.2g/cm2. [•] This improvement is due to the cyclization of 3,6-AG by removing sulfate groups. [•] This study provides an eco-friendly method to produce better quality κ-carrageenan. [ABSTRACT FROM AUTHOR]

MiR-328–3p has been reported to be downregulated and serve as a tumor suppressor in several cancers. Previous studies only have reported the downregulation of miR-328–3p in CRC. However, the roles of miR-328–3p in CRC growth and metastasis were unknown. In this study, we demonstrated that miR-328–3p overexpression inhibited cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT). The PI3K/Akt signaling pathway was also inactivated by miR-328–3p overexpression. MiR-328–3p knockdown showed the opposite effects. In addition, we confirmed that miR-328–3p directly bound to 3′UTR of Girdin and negatively regulated its expression. Girdin knockdown or treatment with PI3K inhibitor LY294002 blocked the effects of miR-328–3p inhibitor on cell proliferation, metastasis, and the PI3K/Akt signaling pathway. Moreover, pre-miR-328 decreased numbers of liver metastatic nodules, and reduced the levels of p-Akt, p-Girdin, and Girdin in metastatic tissues in liver. In conclusion, miR-328–3p may inhibit proliferation and metastasis of CRC cells by targeting Girdin and inactivating the PI3K/Akt signaling pathway. MiR-328–3p may be a novel target in cancer therapy. • MiR-328–3p inhibited cell proliferation and metastasis via Girdin and the PI3K/Akt pathway. • MiR-328–3p knockdown showed the opposite effects. • Girdin is a direct target of miR-328–3p. • Pre-miR-328 suppressed CRC liver metastasis in vivo. [ABSTRACT FROM AUTHOR]

MiR-346-5p is overexpressed in several cancers, including colorectal cancer (CRC). However, the effects of miR-346-5p on CRC progression have not yet been clarified. In our study, miR-346-5p levels in four CRC cell lines and normal human colon epithelial cells were determined by real-time PCR. SW620 and HCT116 cells were selected and then transfected with miR-346-5p mimic, miR-346-5p inhibitor, or specific siRNAs targeting F-box/LRR-repeat protein 2 (FBXL2). Cell proliferation, cell cycle distribution and cell cycle regulators were examined by CCK-8 assay, flow cytometry, and western blot. The binding of miR-346-5p on 3′ untranslated region (UTR) of FBXL2 were verified by dual-luciferase reporter assay. CRC cells were co-transfected with miR-346-5p inhibitor and siFBXL2 to investigate the involvement of FBXL2. Interaction of FBXL2 with forkhead box M1 (FoxM1) was examined by co-immunoprecipitation (Co-IP) assay. The effect of miR-346-5p knockdown on CRC tumorigenesis in vivo was investigated. Here, we found that miR-346-5p overexpression promoted, while miR-346-5p knockdown inhibited cell proliferation and G1-S transition. Inhibition of FBXL2 showed similar effects as miR-346-5p overexpression. Moreover, we verified that FBXL2 was a direct target of miR-346-5p. FBXL2 interacted with FoxM1, and then negatively regulated both FoxM1 and nuclear β-catenin levels. Additionally, FBXL2 knockdown reversed the effects of miR-346-5p inhibitor. In xenograft models, miR-346-5p knockdown significantly inhibited tumor growth, increased FBXL2 expression, and downregulated the levels of FoxM1 and nuclear β-catenin. In conclusion, miR-346-5p may promote CRC growth by targeting FBXL2 and activating the β-catenin signaling pathway. MiR-346-5p may be a novel target in cancer therapy. Unlabelled Image • MiR-346-5p overexpression promoted, while miR-346-5p knockdown inhibited cell proliferation and G1-S transition in vitro. • FBXL2 knockdown showed similar effects as miR-346-5p overexpression on cell proliferation and cell cycle distribution. • FBXL2 interacted with FoxM1, and then negatively regulated both FoxM1 and nuclear β-catenin levels • MiR-346-5p knockdown significantly inhibited tumor growth in vivo. • MiR-346-5p may promote CRC growth by targeting FBXL2 and activating the β-catenin signaling pathway [ABSTRACT FROM AUTHOR]

Huanghe (Yellow River) is one of the largest contributors of global terrestrial materials to the sea. A large amount of the Huanghe-derived sediment are responsible for forming not only the nearshore delta depositional system, but also a distal mud patch off the Shandong Peninsula, some 350 km east of the present-day river mouth. To better understand the spatial and temporal variations of sediment dynamics in the distal mud area, two field surveys employing the phase-averaging method (PAM) were conducted in September 2017 and January 2018, respectively along a 108-km East-West transect across the mud patch. The residual currents derived from the PAM surveys were featured by the southward current in the nearshore area and the northward current in the offshore region in summer, while a unidirectional current transported from south to north across the entire transit line in winter. These patterns, which deviate from the traditional understandings of circulations in magnitude and even in direction, are believed to result from episodic wind reversals and associated strengthening and weakening sea level pressure gradients. The northerly wind episodes in summer, works hand-in-hand with sea-level tilt by preceding southerly wind, to enhance the water exchange between the Bohai Sea and the Yellow Sea by intensifying both the southward flow along the coast and the northward flow of the South Yellow Sea water further offshore. In winter, episodic southerly wind causes relaxation or even reversal of the prevailing winter northerly wind that created the northward sea-level tilt in the Bohai Sea and the Yellow Sea, effectively enhances the South Yellow Sea water transport to the North Yellow Sea and the Bohai Sea. Consequently, a considerable amount of sediment previously exported out of the Bohai Sea to the mud patch actually return with these backflows. Since the synoptic time scale oscillations in the Bohai and Yellow seas are considerably frequent throughout summer and winter, the cumulative effects on the long-term sediment transport need to be further investigated. The observations in this study provide a base for new understanding of how episodic weather events impact the regional circulation and sediment dynamics on the Huanghe-derived distal mud patch. • Residual currents across the Shandong muddy area were examined for the first time. • Nearshore southward current and offshore northward current were evident in summer. • Northerly wind reversals in winter forced northward flowing via the entire section. • Synoptic events could largely change the sediment dynamics in the muddy area. [ABSTRACT FROM AUTHOR]