26 results on '"Panera, Nadia"'
Search Results
2. Cytokine expression patterns in hospitalized children with Bordetella pertussis, Rhinovirus or co-infection.
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Pandolfi, Elisabetta, Panera, Nadia, Alisi, Anna, Carloni, Emanuela, Russo, Luisa, Campagna, Ilaria, Rizzo, Caterina, Concato, Carlo, Linardos, Giulia, Piccioni, Livia, Jackson, Sally, Villani, Alberto, Midulla, Fabio, and Tozzi, Alberto E.
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CYTOKINES , *BORDETELLA pertussis , *PATHOGENIC microorganisms , *RHINOVIRUSES , *INFLAMMATION , *BACTERIAL diseases in children - Abstract
Mechanisms of interaction between Bordetella pertussis and other viral agents are yet to be fully explored. We studied the inflammatory cytokine expression patterns among children with both viral-bacterial infections. Nasopharyngeal aspirate (NPA) samples were taken from children, aged < 1 year, positive for Rhinovirus, Bordetella pertussis and for Rhinovirus and Bordetella pertussis. Forty cytokines were evaluated in NPA by using human cytokine protein arrays and a quantitative analysis was performed on significantly altered cytokines. Forty cytokines were evaluated in NPA by using human cytokine protein arrays and a quantitative analysis was performed on significantly altered cytokines. Our results show that co-infections display a different inflammatory pattern compared to single infections, suggesting that a chronic inflammation caused by one of the two pathogens could be the trigger for exacerbation in co-infections. [ABSTRACT FROM AUTHOR]
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- 2021
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3. The G-Quadruplex/Helicase World as a Potential Antiviral Approach Against COVID-19.
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Panera, Nadia, Tozzi, Alberto Eugenio, and Alisi, Anna
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ENZYME analysis , *ANTIVIRAL agents , *DNA , *GENOMES , *LIGANDS (Biochemistry) , *MOLECULAR structure , *RNA , *SARS disease , *COVID-19 , *PHARMACODYNAMICS - Abstract
G-Quadruplexes (G4s) are non-canonical secondary structures formed within guanine-rich regions of DNA or RNA. G4 sequences/structures have been detected in human and in viral genomes, including Coronaviruses Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and SARS-CoV-2. Here, we outline the existing evidence indicating that G4 ligands and inhibitors of SARS-CoV-2 helicase may exert some antiviral activity reducing viral replication and can represent a potential therapeutic approach to tackle the COVID-19 pandemic due to SARS-CoV-2 infection. We also discuss how repositioning of FDA-approved drugs against helicase activity of other viruses, could represent a rapid strategy to limit deaths associated with COVID-19 pandemic. [ABSTRACT FROM AUTHOR]
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- 2020
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4. A review of the pathogenic and therapeutic role of nutrition in pediatric nonalcoholic fatty liver disease.
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Panera, Nadia, Barbaro, Barbara, Della Corte, Claudia, Mosca, Antonella, Nobili, Valerio, and Alisi, Anna
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VITAMIN therapy , *DOCOSAHEXAENOIC acid , *THERAPEUTIC use of probiotics , *ANTIGENS , *DIETARY supplements , *FATTY liver , *MEDLINE , *NUTRITION , *ONLINE information services , *SYSTEMATIC reviews , *CHILDREN , *THERAPEUTICS - Abstract
Abstract Nonalcoholic fatty liver disease (NAFLD) is a multifaceted disorder that ranges from simple fatty liver to nonalcoholic steatohepatitis (NASH) with or without fibrosis, which may evolve toward cirrhosis and hepatocellular carcinoma. It is currently considered a "global" and "epidemic" disease, whose prevalence is progressively increasing even in pediatric age. The incidence of NAFLD is very high in overweight/obese children, and a greater risk of disease progression is associated with severe obesity, highlighting the role of nutrition. To date, for NAFLD, there are few guidelines for diagnostic and follow-up methods, and scarce validated protocols for treatment. The initial indications consist of gradual weight loss and regular exercise, but in children, the difficulty of adhering to long-term behavioral changes makes this approach limited. The purpose of this narrative review is to examine the mechanism underlying the pathogenic mechanisms that lead to NAFLD in children, with a major focus on the role of nutrition. Because this is particularly relevant in light of the absence of pharmacological treatments suitable for children, we also overview clinical studies on the potential effects of nutritional supplementations, including vitamins, docosahexaenoic acid, and probiotics.in children. To this aim, updated search was conducted on PubMed and clinicaltrials.gov databases. Future research should consider additional clinical studies in pediatric NAFLD patients to validate the benefits of dietary supplements and to define the appropriate dosage and duration for intervention. Furthermore, experimental studies with -omics approaches could be helpful to deepen the related mechanisms and to search for a possible optimal supplement combination against NAFLD in children. [ABSTRACT FROM AUTHOR]
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- 2018
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5. From pregnant women to infants: Non-alcoholic fatty liver disease is a poor inheritance.
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Mosca, Antonella, Panera, Nadia, Maggiore, Giuseppe, and Alisi, Anna
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LIVER histology , *PREGNANT women , *NON-alcoholic fatty liver disease , *ANKYLOGLOSSIA , *FETAL growth retardation , *INFANTS , *TYPE 2 diabetes - Published
- 2020
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6. Focal Adhesion Kinase: Insight into Molecular Roles and Functions in Hepatocellular Carcinoma.
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Panera, Nadia, Crudele, Annalisa, Romito, Ilaria, Gnani, Daniela, and Alisi, Anna
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LIVER cancer , *FOCAL adhesion kinase , *GENETIC mutation , *ANTINEOPLASTIC agents , *P53 antioncogene - Abstract
Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide. Due to the high incidence of post-operative recurrence after current treatments, the identification of new and more effective drugs is required. In previous years, new targetable genes/pathways involved in HCC pathogenesis have been discovered through the help of high-throughput sequencing technologies. Mutations in TP53 and β-catenin genes are the most frequent aberrations in HCC. However, approaches able to reverse the effect of these mutations might be unpredictable. In fact, if the reactivation of proteins, such as p53 in tumours, holds great promise as anticancer therapy, there are studies arguing that chronic activation of these types of molecules may be deleterious. Thus, recently the efforts on potential targets have focused on actionable mutations, such as those occurring in the gene encoding for focal adhesion kinase (FAK). This tyrosine kinase, localized to cellular focal contacts, is over-expressed in a variety of human tumours, including HCC. Moreover, several lines of evidence demonstrated that FAK depletion or inhibition impair in vitro and in vivo HCC growth and metastasis. Here, we provide an overview of FAK expression and activity in the context of tumour biology, discussing the current evidence of its connection with HCC development and progression. [ABSTRACT FROM AUTHOR]
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- 2017
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7. High concentrations of H2O2 trigger hypertrophic cascade and phosphatase and tensin homologue (PTEN) glutathionylation in H9c2 cardiomyocytes.
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Panera, Nadia, Gnani, Daniela, Piermarini, Emanuela, Petrini, Stefania, Bertini, Enrico, Nobili, Valerio, Pastore, Anna, Piemonte, Fiorella, and Alisi, Anna
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HYDROGEN peroxide , *HYPERTROPHY , *PHOSPHATASES , *HEART cells , *CARDIAC hypertrophy - Abstract
Cardiac hypertrophy occurs in response to different stimuli and is mainly characterized by an enlargement of cardiomyocyte size. During hypertrophy, cardiomyocytes undergo not only radical changes of the cellular architecture but also activation of signaling cascades that counteract the atrophy genes. Experimental studies highlighted that chronic low concentrations of H 2 O 2 , induce a hypertrophic phenotype, while higher levels of H 2 O 2 promote apoptosis. In this study, we explored the early and long-term hypertrophic effects of high concentrations of H 2 O 2 on H9c2 rat cardiomyocytes. We found that 2-h stimulation with 200 μM H 2 O 2 caused an early dramatic reduction of cell viability, accompanied, 5-days later, by increased cell size and up-regulation of atrial natriuretic peptide transcription. This hypertrophic phenotype is associated to increased Akt phosphorylation and a consequent reduction of the FOXO3a and atrogin-1 gene expression. Moreover, we observed that H 2 O 2 caused the overexpression of miR-212/miR-132 cluster concomitantly to a down-regulation of PTEN transcript without changes in its protein expression. Noteworthy, we found that the treatment of cardiomyocytes with H 2 O 2 further led to an increase of oxidized glutathione and glutathionylation of proteins, including PTEN. In conclusion, our results permit to reconstruct the molecular cascade triggering the cardiomyocyte hypertrophy upon high concentrations of H 2 O 2 . [ABSTRACT FROM AUTHOR]
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- 2016
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8. Dual Role of MicroRNAs in NAFLD.
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Ceccarelli, Sara, Panera, Nadia, Gnani, Daniela, and Nobili, Valerio
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MICRORNA , *GENETIC transcription , *FATTY liver , *PATHOLOGICAL physiology , *GENETIC translation , *GENE targeting , *GENE expression - Abstract
MicroRNAs are important post-transcriptional regulators in different pathophysiological processes. They typically affect the mRNA stability or translation finally leading to the repression of target gene expression. Notably, it is thought that microRNAs are crucial for regulating gene expression during metabolic-related disorders, such as nonalcoholic fatty liver disease (NAFLD). Several studies identify specific microRNA expression profiles associated to different histological features of NAFLD, both in animal models and in patients. Therefore, specific assortments of certain microRNAs could have enormous diagnostic potentiality. In addition, microRNAs have also emerged as possible therapeutic targets for the treatment of NAFLD-related liver damage. In this review, we discuss the experimental evidence about microRNAs both as potential non-invasive early diagnostic markers and as novel therapeutic targets in NAFLD and its more severe liver complications. [ABSTRACT FROM AUTHOR]
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- 2013
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9. Intrauterine Growth Retardation and Nonalcoholic Fatty Liver Disease in Children.
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Alisi, Anna, Panera, Nadia, Agostoni, Carlo, and Nobili, Valerio
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FETAL growth retardation , *FATTY liver , *JUVENILE diseases , *INSULIN resistance , *GESTATIONAL age - Abstract
Intrauterine growth retardation (IUGR), the most important cause of perinatal mortality and morbidity, is defined as a foetal growth less than normal for the population, often used as synonym of small for gestational age (SGA). Studies demonstrated the relationships between metabolic syndrome (MS) and birthweight. This study suggested that, in children, adolescents, and adults born SGA, insulin resistance could lead to other metabolic disorders: type 2 diabetes (DM2), dyslipidemia, and nonalcoholic fatty liver disease (NAFLD). NAFLD may evolve to nonalcoholic steatohepatitis (NASH), and it is related to the development of MS. Lifestyle intervention, physical activity, and weight reduction represent the mainstay of NAFLD therapy. In particular, a catch-up growth reduction could decrease the risk to develop MS and NAFLD. In this paper, we outline clinical and experimental evidences of the association between IUGR, metabolic syndrome, insulin resistance, and NAFLD and discuss on a possible management to avoid the risk of MS in adulthood. [ABSTRACT FROM AUTHOR]
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- 2011
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10. Phosphodiesterase 4D Depletion/Inhibition Exerts Anti-Oncogenic Properties in Hepatocellular Carcinoma.
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Ragusa, Federica, Panera, Nadia, Cardarelli, Silvia, Scarsella, Marco, Bianchi, Marzia, Biagioni, Stefano, Giorgi, Mauro, Alisi, Anna, Massimi, Mara, and Tiribelli, Claudio
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IN vitro studies , *IN vivo studies , *ANTINEOPLASTIC agents , *APOPTOSIS , *GENE expression , *INSULIN , *FLUORESCENT antibody technique , *CELL lines , *POLYMERASE chain reaction , *HEPATOCELLULAR carcinoma , *PHOSPHODIESTERASE inhibitors - Abstract
Simple Summary: Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related mortality worldwide. Drug resistance is a serious problem in the treatment of HCC. Therefore, it is of high clinical impact to discover targeted therapies that may overcome drug-related resistance and improve the survival of patients affected by HCC. In the present study, we investigated the role of Isoform D of type 4 phosphodiesterase (PDE4D) in HCC development and progression. We found that PDE4D is over-expressed HCCs in vitro and in vivo and the depletion of the gene by silencing or the pharmacological inhibition of protein activity exerted anti-tumorigenic activities. Isoform D of type 4 phosphodiesterase (PDE4D) has recently been associated with several human cancer types with the exception of human hepatocellular carcinoma (HCC). Here we explored the role of PDE4D in HCC. We found that PDE4D gene/protein were over-expressed in different samples of human HCCs compared to normal livers. Accordingly, HCC cells showed higher PDE4D activity than non-tumorigenic cells, accompanied by over-expression of the PDE4D isoform. Silencing of PDE4D gene and pharmacological inhibition of protein activity by the specific inhibitor Gebr-7b reduced cell proliferation and increased apoptosis in HCC cells, with a decreased fraction of cells in S phase and a differential modulation of key regulators of cell cycle and apoptosis. PDE4D silencing/inhibition also affected the gene expression of several cancer-related genes, such as the pro-oncogenic insulin growth factor (IGF2), which is down-regulated. Finally, gene expression data, available in the CancerLivER data base, confirm that PDE4D over-expression in human HCCs correlated with an increased expression of IGF2, suggesting a new possible molecular network that requires further investigations. In conclusion, intracellular depletion/inhibition of PDE4D prevents the growth of HCC cells, displaying anti-oncogenic effects. PDE4D may thus represent a new biomarker for diagnosis and a potential adjuvant target for HCC therapy. [ABSTRACT FROM AUTHOR]
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- 2021
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11. The Number of Liver Galectin-3 Positive Cells Is Dually Correlated with NAFLD Severity in Children.
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de Oliveira, Felipe Leite, Panera, Nadia, De Stefanis, Cristiano, Mosca, Antonella, D'Oria, Valentina, Crudele, Annalisa, De Vito, Rita, Nobili, Valerio, and Alisi, Anna
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LIVER cells , *FATTY liver , *LIVER - Abstract
Non-alcoholic fatty liver disease (NAFLD) is a complex disease ranging from steatosis to non-alcoholic steatohepatitis (NASH). Galectin-3 (Gal-3), which is a β-galactoside binding protein, has been associated with liver fibrosis, but its role in NAFLD remains elusive. We investigated the expression of Gal-3 in liver resident cells and its potential association with liver damage in 40 children with biopsy-proven NAFLD. We found that several liver cells expressed Gal-3. The number of total Gal-3 positive cells decreased with the severity of disease and the cells were correlated with the presence of steatosis and the diagnosis of NASH. CD68 macrophages expressed Gal-3 but the number CD68/Gal-3 positive cells was significantly reduced in patients diagnosed with steatosis and NASH. Triple CD68/CD206/Gal-3, which represented the subpopulation of M2 macrophages, were mainly present in patients without NASH, and clearly reduced in patients with steatosis and NASH. On the contrary, the number of α-smooth muscle actin (SMA)/Gal-3 positive cells increased with the severity of fibrosis in children with NAFLD. Our data demonstrated that the number of Gal-3 positive cells was associated with tissue damage in different ways, which suggests a dual role of this protein in the pathogenesis of pediatric NAFLD, even if the role of Gal-3 deserves further studies. [ABSTRACT FROM AUTHOR]
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- 2019
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12. β-Klotho gene variation is associated with liver damage in children with NAFLD.
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Dongiovanni, Paola, Crudele, Annalisa, Panera, Nadia, Romito, Ilaria, Meroni, Marica, De Stefanis, Cristiano, Palma, Alessia, Comparcola, Donatella, Fracanzani, Anna Ludovica, Miele, Luca, Valenti, Luca, Nobili, Valerio, and Alisi, Anna
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FATTY liver , *FIBROBLAST growth factors , *PEDIATRIC nephrology , *FREE fatty acids , *PATHOLOGY , *JUVENILE diseases - Abstract
• The KLB rs17618244 variant increases the risk of ballooning and lobular inflammation in children with NAFLD. • KLB plasma levels are lower in carriers of the rs17618244 minor A allele. • KLB plasma levels are associated with lobular inflammation, ballooning and fibrosis. • KLB mutant induces intracellular lipid accumulation in HepG2 and Huh7. • KLB mutant causes upregulation of lipotoxic and proinflammatory genes. Non-alcoholic fatty liver disease (NAFLD) is the leading cause of chronic liver disease in adults and children. Along with obesity, diabetes and insulin resistance, genetic factors strongly impact on NAFLD development and progression. Dysregulated bile acid metabolism and the fibroblast growth factor 19 (FGF19) pathway play a pivotal role in NAFLD pathogenesis. However, the mechanism through which the FGF19 receptor system is associated with liver damage in NAFLD remains to be defined. We evaluated the impact of the rs17618244 G>A β-Klotho (KLB) variant on liver damage in 249 pediatric patients with biopsy-proven NAFLD and the association of this variant with the expression of hepatic and soluble KLB. In vitro models were established to investigate the role of the KLB mutant. The KLB rs17618244 variant was associated with an increased risk of ballooning and lobular inflammation. KLB plasma levels were lower in carriers of the rs17618244 minor A allele and were associated with lobular inflammation, ballooning and fibrosis. In HepG2 and Huh7 hepatoma cell lines, exposure to free fatty acids caused a severe reduction of intracellular and secreted KLB. Finally, KLB downregulation obtained by the expression of a KLB mutant in HepG2 and Huh7 cells induced intracellular lipid accumulation and upregulation of p62, ACOX1, ACSL1, IL-1β and TNF-α gene expression. In conclusion, we showed an association between the rs17618244 KLB variant, which leads to reduced KLB expression, and the severity of NAFLD in pediatric patients. We can speculate that the KLB protein may exert a protective role against lipotoxicity and inflammation in hepatocytes. Genetic and environmental factors strongly impact on the pathogenesis and progression of non-alcoholic fatty liver disease (NAFLD). The FGF19/FGFR4/KLB pathway plays a pivotal role in the pathogenesis of NAFLD. The aim of the study was to investigate the impact of a genetic variant in the KLB gene on the severity of liver disease. Our data suggest that the KLB protein plays a protective role against lipotoxicity and inflammation in hepatocytes. [ABSTRACT FROM AUTHOR]
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- 2020
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13. Dual role of survivin in non-alcoholic fatty liver disease.
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Panera, Nadia, Raso, Roberto, Nobili, Valerio, and Alisi, Anna
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FATTY liver , *SURVIVIN (Protein) - Abstract
A letter to the editor is presented related to association between non-alcoholic fatty liver disease and survivin.
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- 2011
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14. Correction to: Focal adhesion kinase inhibitor TAE226 combined with Sorafenib slows down hepatocellular carcinoma by multiple epigenetic effects.
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Romito, Ilaria, Porru, Manuela, Braghini, Maria Rita, Pompili, Luca, Panera, Nadia, Crudele, Annalisa, Gnani, Daniela, De Stefanis, Cristiano, Scarsella, Marco, Pomella, Silvia, Mortera, Stefano Levi, de Billy, Emmanuel, Conti, Adrian Libenzio, Marzano, Valeria, Putignani, Lorenza, Vinciguerra, Manlio, Balsano, Clara, Pastore, Anna, Rota, Rossella, and Tartaglia, Marco
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FOCAL adhesion kinase , *KINASE inhibitors , *HEPATOCELLULAR carcinoma , *SORAFENIB , *EPIGENETICS - Abstract
An amendment to this paper has been published and can be accessed via the original article. [ABSTRACT FROM AUTHOR]
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- 2022
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15. Association between Serum Atypical Fibroblast Growth Factors 21 and 19 and Pediatric Nonalcoholic Fatty Liver Disease.
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Alisi, Anna, Ceccarelli, Sara, Panera, Nadia, Prono, Federica, Petrini, Stefania, De Stefanis, Cristiano, Pezzullo, Marco, Tozzi, Alberto, Villani, Alberto, Bedogni, Giorgio, and Nobili, Valerio
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FIBROBLAST growth factors , *SERUM , *FATTY liver , *THERAPEUTICS , *JUVENILE diseases , *ENERGY metabolism , *COMPARATIVE studies , *DISEASE progression - Abstract
Atypical fibroblast growth factors (FGF) 21 and 19 play a central role in energy metabolism through the mediation of Klotho coreceptor. Contradictory findings are available about the association of FGF21 and FGF19 with nonalcoholic fatty liver disease (NAFLD) in humans. We investigated the association of serum FGF21, FGF19 and liver Klotho coreceptor with non-alcoholic steatohepatitis (NASH) and fibrosis in children with NAFLD. Serum FGF21 and FGF19 were measured in 84 children with biopsy-proven NAFLD and 23 controls (CTRL). The hepatic expression of Klotho coreceptor was measured in 7 CTRL, 9 patients with NASH (NASH+) and 11 patients without NASH (NASH−). FGF21 and FGF19 showed a tendency to decrease from CTRL (median FGF21 = 196 pg/mL; median FGF19 = 201 pg/mL) to NASH− (FGF21 = 89 pg/mL; FGF19 = 81 pg/mL) to NASH+ patients (FGF21 = 54 pg/mL; FGF19 = 41 pg/mL) (p<0.001 for all comparisons) and were inversely associated with the probability of NASH and fibrosis in children with NAFLD. The hepatic expression of Klotho coreceptor was inversely associated with NASH (R2 = 0.87, p<0.0001) and directly associated with serum FGF21 (R2 = 0.57, p<0.0001) and FGF19 (R2 = 0.67, p<0.0001). In conclusion, serum FGF19 and FGF21 and hepatic Klotho expression are inversely associated with hepatic damage in children with NAFLD and these findings may have important implications for understanding the mechanisms of NAFLD progression. [ABSTRACT FROM AUTHOR]
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- 2013
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16. Focal adhesion kinase inhibitor TAE226 combined with Sorafenib slows down hepatocellular carcinoma by multiple epigenetic effects.
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Romito, Ilaria, Porru, Manuela, Braghini, Maria Rita, Pompili, Luca, Panera, Nadia, Crudele, Annalisa, Gnani, Daniela, De Stefanis, Cristiano, Scarsella, Marco, Pomella, Silvia, Levi Mortera, Stefano, de Billy, Emmanuel, Conti, Adrian Libenzio, Marzano, Valeria, Putignani, Lorenza, Vinciguerra, Manlio, Balsano, Clara, Pastore, Anna, Rota, Rossella, and Tartaglia, Marco
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FOCAL adhesion kinase , *SORAFENIB , *KINASE inhibitors , *HEPATOCELLULAR carcinoma , *EPIGENETICS , *OVERALL survival - Abstract
Background: Hepatocellular carcinoma (HCC) is one of the most common and lethal malignant tumours worldwide. Sorafenib (SOR) is one of the most effective single-drug systemic therapy against advanced HCC, but the identification of novel combination regimens for a continued improvement in overall survival is a big challenge. Recent studies highlighted the crucial role of focal adhesion kinase (FAK) in HCC growth. The aim of this study was to investigate the antitumor effects of three different FAK inhibitors (FAKi), alone or in combination with SOR, using in vitro and in vivo models of HCC. Methods: The effect of PND1186, PF431396, TAE226 on cell viability was compared to SOR. Among them TAE226, emerging as the most effective FAKi, was tested alone or in combination with SOR using 2D/3D human HCC cell line cultures and HCC xenograft murine models. The mechanisms of action were assessed by gene/protein expression and imaging approaches, combined with high-throughput methods. Results: TAE226 was the more effective FAKi to be combined with SOR against HCC. Combined TAE226 and SOR treatment reduced HCC growth both in vitro and in vivo by affecting tumour-promoting gene expression and inducing epigenetic changes via dysregulation of FAK nuclear interactome. We characterized a novel nuclear functional interaction between FAK and the NuRD complex. TAE226-mediated FAK depletion and SOR-promoted MAPK down-modulation caused a decrease in the nuclear amount of HDAC1/2 and a consequent increase of the histone H3 lysine 27 acetylation, thus counteracting histone H3 lysine 27 trimethylation. Conclusions: Altogether, our findings provide the first evidence that TAE226 combined with SOR efficiently reduces HCC growth in vitro and in vivo. Also, our data highlight that deep analysis of FAK nuclear interactome may lead to the identification of new promising targets for HCC therapy. [ABSTRACT FROM AUTHOR]
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- 2021
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17. HDL cholesterol protects from liver injury in mice with intestinal specific LXRα activation.
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Pierantonelli, Irene, Lioci, Gessica, Gurrado, Fabio, Giordano, Debora M., Rychlicki, Chiara, Bocca, Claudia, Trozzi, Luciano, Novo, Erica, Panera, Nadia, De Stefanis, Cristiano, D'Oria, Valentina, Marzioni, Marco, Maroni, Luca, Parola, Maurizio, Alisi, Anna, and Svegliati‐Baroni, Gianluca
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LIVER injuries , *INTESTINAL injuries , *KUPFFER cells , *HDL cholesterol , *CARBON tetrachloride - Abstract
Background and aims: Liver X receptors (LXRs) exert anti‐inflammatory effects even though their hepatic activation is associated with hypertriglyceridemia and hepatic steatosis. Selective induction of LXRs in the gut might provide protective signal(s) in the aberrant wound healing response that induces fibrosis during chronic liver injury, without hypertriglyceridemic and steatogenic effects. Methods: Mice with intestinal constitutive LXRα activation (iVP16‐LXRα) were exposed to intraperitoneal injection of carbon tetrachloride (CCl4) for 8 weeks, and in vitro cell models were used to evaluate the beneficial effect of high‐density lipoproteins (HDL). Results: After CCl4 treatment, the iVP16‐LXRα phenotype showed reduced M1 macrophage infiltration, increased expression M2 macrophage markers, and lower expression of hepatic pro‐inflammatory genes. This anti‐inflammatory effect in the liver was also associated with decreased expression of hepatic oxidative stress genes and reduced expression of fibrosis markers. iVP16‐LXRα exhibited increased reverse cholesterol transport in the gut by ABCA1 expression and consequent enhancement of the levels of circulating HDL and their receptor SRB1 in the liver. No hepatic steatosis development was observed in iVP16‐LXRα. In vitro, HDL induced a shift from M1 to M2 phenotype of LPS‐stimulated Kupffer cells, decreased TNFα‐induced oxidative stress in hepatocytes and reduced NF‐kB activity in both cells. SRB1 silencing reduced TNFα gene expression in LPS‐stimulated KCs, and NOX‐1 and IL‐6 in HepG2. Conclusions: Intestinal activation of LXRα modulates hepatic response to injury by increasing circulating HDL levels and SRB1 expression in the liver, thus suggesting this circuit as potential actionable pathway for therapy. [ABSTRACT FROM AUTHOR]
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- 2020
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18. AS140 - HDL cholesterol blocks NF-kB activation and M1 macrophages transformation thus reducing hepatic fibrosis in mice with selective intestinal LXR-alpha activation.
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Lioci, Gessica, Gurrado, Fabio, Panera, Nadia, De Stefanis, Cristiano, Doria, Valentina, Bocca, Claudia, Novo, Erica, Pierantonelli, Irene, Parola, Maurizio, Alisi, Anna, and Svegliati-Baroni, Gianluca
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HEPATIC fibrosis , *NF-kappa B , *MACROPHAGE activation , *HDL cholesterol , *MICE - Published
- 2020
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19. Harnessing Omics Approaches on Advanced Preclinical Models to Discovery Novel Therapeutic Targets for the Treatment of Metastatic Colorectal Cancer.
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Porru, Manuela, Zizza, Pasquale, Panera, Nadia, Alisi, Anna, Biroccio, Annamaria, and Leonetti, Carlo
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BIOLOGICAL models , *COLON tumors , *DRUG design , *EPIDERMAL growth factor , *METASTASIS , *CHEMICAL inhibitors ,RECTUM tumors - Abstract
Metastatic colorectal cancer (mCRC) remains challenging because of the emergence of resistance mechanisms to anti-epidermal growth factor receptor (EGFR) therapeutics, so more effective strategies to improve the patients' outcome are needed. During the last decade, the application of a multi-omics approach has contributed to a deeper understanding of the complex molecular landscape of human CRC, identifying a plethora of drug targets for precision medicine. Target validation relies on the use of experimental models that would retain the molecular and clinical features of human colorectal cancer, thus mirroring the clinical characteristics of patients. In particular, organoids and patient-derived-xenografts (PDXs), as well as genetically engineered mouse models (GEMMs) and patient-derived orthotopic xenografts (PDOXs), should be considered for translational purposes. Overall, omics and advanced mouse models of cancer represent a portfolio of sophisticated biological tools that, if optimized for use in concert with accurate data analysis, could accelerate the anticancer discovery process and provide new weapons against cancer. In this review, we highlight success reached following the integration of omics and experimental models; moreover, results produced by our group in the field of mCRC are also presented. [ABSTRACT FROM AUTHOR]
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- 2020
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20. Does Nox2 Overactivate in Children with Nonalcoholic Fatty Liver Disease?
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Loffredo, Lorenzo, Zicari, Anna Maria, Perri, Ludovica, Carnevale, Roberto, Nocella, Cristina, Angelico, Francesco, Del Ben, Maria, Mosca, Antonella, Zaffina, Salvatore, Panera, Nadia, Alisi, Anna, Duse, Marzia, Violi, Francesco, and Nobili, Valerio
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FATTY liver , *NADPH oxidase , *ENZYME activation , *OXIDATIVE stress , *LIPOPOLYSACCHARIDES - Abstract
It is unknown whether nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (Nox2) activation is early associated with endotoxemia and liver damage in nonalcoholic fatty liver disease (NAFLD). To address this issue, we evaluated Nox2 activation, oxidative stress, gut permeability, and lipopolysaccharide (LPS) serum levels in 67 children with biopsy-proven NAFLD and 73 controls. Compared with controls, NAFLD patients had higher Nox2 activity, isoprostane, zonulin, and LPS levels. Multivariate linear regression analysis showed that triglycerides, high-density lipoprotein (HDL), homeostatic model assessment-estimated insulin resistance (HOMA-IR), LPS, and isoprostanes were independently associated with Nox2-derivative peptide (sNox2-dp) levels. Within the NAFLD group, patients with nonalcoholic steatohepatitis (NASH) had significant higher levels of sNox2-dp, isoprostanes, LPS, triglycerides, HOMA-IR, fasting glucose and insulin, and lower HDL than those without NASH. Furthermore, sNox2-dp levels were linearly associated with the histological grading of steatosis, inflammation, ballooning, fibrosis, and NAFLD activity score. This study provides evidence that children with NAFLD have Nox2 overactivation compared with controls and significant association with the degree of liver damage. The close relationship between Nox2 and LPS serum levels leads to hypothesize a potential role for gut-derived LPS in eliciting systemic Nox2 activation. [ABSTRACT FROM AUTHOR]
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- 2019
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21. Expression of insulin-like growth factor I and its receptor in the liver of children with biopsy-proven NAFLD.
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Alisi, Anna, Pampanini, Valentina, De Stefanis, Cristiano, Panera, Nadia, Deodati, Annalisa, Nobili, Valerio, and Cianfarani, Stefano
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FATTY liver , *SOMATOMEDIN C , *PEDIATRICS , *IMMUNOFLUORESCENCE , *MESSENGER RNA - Abstract
Background and aims: Nonalcoholic fatty liver disease is one of the major complications of obesity, occurring already in pediatric age. Insulin like growth factor-I has been proposed as a potential therapeutic agent for its beneficial effect in experimental liver fibrosis. The aim of this work was to investigate the expression of insulin-like growth factor-I and its receptor in the liver of children with biopsy-proven nonalcoholic fatty liver disease and relate it to liver histological features. Methods: 45 obese children and adolescents (14 females and 31 males) with nonalcoholic fatty liver disease were included. Insulin like growth factor-I and its receptor expression was evaluated in liver tissue by immunofluorescence and qPCR. Results: The expression of insulin like growth factor-I and its receptor were significantly related to fibrosis and were higher in children with stage 3 fibrosis compared to stage 1 and 2 (p<0.001 and p = 0.007 respectively). mRNA of insulin like growth factor-I receptor was higher in more advanced stages of fibrosis (p<0.001). Furthermore, the expression of insulin like growth factor-I and its receptor in hepatic stellate cells, the cell type mostly involved in fibrosis progression, was significantly increased in stage 3 fibrosis compared to stage 1 (p = 0.01 and p = 0.008 respectively). Conclusions: We demonstrated for the first time that insulin like growth factor-I and its receptor are upregulated in children with nonalcoholic fatty liver disease. These findings give a new hint for the potential therapeutic use of insulin like growth factor-I in pediatric nonalcoholic fatty liver disease complicated by liver fibrosis. [ABSTRACT FROM AUTHOR]
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- 2018
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22. The Role of Tissue Macrophage-Mediated Inflammation on NAFLD Pathogenesis and Its Clinical Implications.
- Author
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Alisi, Anna, Carpino, Guido, Oliveira, Felipe L., Panera, Nadia, Nobili, Valerio, and Gaudio, Eugenio
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FATTY liver , *MACROPHAGES , *SYSTEMIC inflammatory response syndrome , *FAT cells , *ADIPOSE tissues , *DISEASE progression - Abstract
The obese phenotype is characterized by a state of chronic low-grade systemic inflammation that contributes to the development of comorbidities, including nonalcoholic fatty liver disease (NAFLD). In fact, NAFLD is often associated with adipocyte enlargement and consequent macrophage recruitment and inflammation. Macrophage polarization is often associated with the proinflammatory state in adipose tissue. In particular, an increase of M1 macrophages number or of M1/M2 ratio triggers the production and secretion of various proinflammatory signals (i.e., adipocytokines). Next, these inflammatory factors may reach the liver leading to local M1/M2 macrophage polarization and consequent onset of the histological damage characteristic of NAFLD. Thus, the role of macrophage polarization and inflammatory signals appears to be central for pathogenesis and progression of NAFLD, even if the heterogeneity of macrophages and molecular mechanisms that govern their phenotype switch remain incompletely understood. In this review, we discuss the role of adipose and liver tissue macrophage-mediated inflammation in experimental and human NAFLD. This focus is relevant because it may help researchers that approach clinical and experimental studies on this disease advancing the knowledge of mechanisms that could be targeted in order to revert NAFLD-related fibrosis. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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23. Plasma Cathepsin D Levels: A Novel Tool to Predict Pediatric Hepatic Inflammation.
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Walenbergh, Sofie M A, Houben, Tom, Hendrikx, Tim, Jeurissen, Mike L J, van Gorp, Patrick J, Vreugdenhil, Anita C E, Adriaanse, Marlou P, Buurman, Wim A, Hofker, Marten H, Mosca, Antonella, Lindsey, Patrick J, Alisi, Anna, Liccardo, Daniela, Panera, Nadia, Koek, Ger H, Nobili, Valerio, and Shiri-Sverdlov, Ronit
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CATHEPSIN D , *PEDIATRIC diagnosis , *FATTY liver , *PROTEASE inhibitors , *ALANINE aminotransferase - Abstract
OBJECTIVES:Nonalcoholic steatohepatitis (NASH) is the most severe form of a hepatic condition known as nonalcoholic fatty liver disease (NAFLD). NASH is histologically characterized by hepatic fat accumulation, inflammation, and ballooning, and eventually coupled with fibrosis that, in turn, may progress to end-stage liver disease even in young individuals. Hence, there is a critical need for specific noninvasive markers to predict hepatic inflammation at an early age. We investigated whether plasma levels of cathepsin D (CatD), a lysosomal protease, correlated with the severity of liver inflammation in pediatric NAFLD.METHODS:Liver biopsies from children (n=96) with NAFLD were histologically evaluated according to the criteria of Kleiner (NAFLD activity score) and the Brunt's criteria. At the time of liver biopsy, blood was taken and levels of CatD, alanine aminotransferase (ALT), and cytokeratin-18 (CK-18) were measured in plasma.RESULTS:Plasma CatD levels were significantly lower in subjects with liver inflammation compared with steatotic subjects. Furthermore, we found that CatD levels were gradually reduced and corresponded with increasing severity of liver inflammation, steatosis, hepatocellular ballooning, and NAFLD activity score. CatD levels correlated with pediatric NAFLD disease progression better than ALT and CK-18. In particular, CatD showed a high diagnostic accuracy (area under receiver operating characteristic curve (ROC-AUC): 0.94) for the differentiation between steatosis and hepatic inflammation, and reached almost the maximum accuracy (ROC-AUC: 0.998) upon the addition of CK-18.CONCLUSIONS:Plasma CatD holds a high diagnostic value to distinguish pediatric patients with hepatic inflammation from children with steatosis. [ABSTRACT FROM AUTHOR]
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- 2015
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24. Plasma Levels of Homocysteine and Cysteine Increased in Pediatric NAFLD and Strongly Correlated with Severity of Liver Damage.
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Pastore, Anna, Alisi, Anna, Giovamberardino, Gianna di, Crudele, Annalisa, Ceccarelli, Sara, Panera, Nadia, Dionisi-Vici, Carlo, and Nobili, Valerio
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HIGH performance liquid chromatography , *FATTY liver , *THERAPEUTICS , *OXIDATIVE stress , *INFLAMMATION , *LIVER cells , *PATIENTS - Abstract
Non-alcoholic fatty liver disease (NAFLD) is a spectrum of metabolic abnormalities ranging from simple triglyceride accumulation in the hepatocytes to hepatic steatosis with inflammation, ballooning and fibrosis. It has been demonstrated that the pathogenesis of NAFLD involves increased oxidative stress, with consumption of the major cellular antioxidant, glutathione (GSH). Liver has a fundamental role in sulfur compound metabolism, although the data reported on plasma thiols status in NAFLD are conflicting. We recruited 63 NAFLD patients, and we analyzed all plasma thiols, such as homocysteine (Hcy), cysteine (Cys), cysteinylglycine (CysGly) and GSH, by high-performance liquid chromatography (HPLC) with fluorescence detection. Hcy, Cys and CysGly plasma levels increased in NAFLD patients (p < 0.0001); whereas GSH levels were decreased in NAFLD patients when compared to controls (p < 0.0001). On the contrary, patients with steatohepatitis exhibited lower levels of Hcy and Cys than subjects without. Furthermore, a positive correlation was found between Hcy and Cys and the presence of fibrosis in children with NAFLD. Taken together, these data demonstrated a defective hepatic sulfur metabolism in children with NAFLD, and that high levels of Hcy and Cys probably correlates with a pattern of more severe histological liver damage, due to mechanisms that require further studies. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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25. Emodin Prevents Intrahepatic Fat Accumulation, Inflammation and Redox Status Imbalance During Diet-Induced Hepatosteatosis in Rats.
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Alisi, Anna, Pastore, Anna, Ceccarelli, Sara, Panera, Nadia, Gnani, Daniela, Bruscalupi, Giovannella, Massimi, Mara, Tozzi, Giulia, Piemonte, Fiorella, and Nobili, Valerio
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EMODIN , *INFLAMMATION , *LABORATORY rats , *HIGH-fat diet , *HIGH-carbohydrate diet , *FATTY liver , *FATTY degeneration , *PREVENTION - Abstract
High-fat and/or high-carbohydrate diets may predispose to several metabolic disturbances including liver fatty infiltration (hepatosteatosis) or be associated with necro-inflammation and fibrosis (steatohepatitis). Several studies have emphasized the hepatoprotective effect of some natural agents. In this study, we investigated the potential therapeutic effects of the treatment with emodin, an anthraquinone derivative with anti-oxidant and anti-cancer abilities, in rats developing diet-induced hepatosteatosis and steatohepatitis. Sprague-Dawley rats were fed a standard diet (SD) for 15 weeks, or a high-fat/high-fructose diet (HFD/HF). After 5 weeks, emodin was added to the drinking water of some of the SD and HFD/HF rats. The experiment ended after an additional 10 weeks. Emodin-treated HFD/HF rats were protected from hepatosteatosis and metabolic derangements usually observed in HFD/HF animals. Furthermore, emodin exerted anti-inflammatory activity by inhibiting the HFD/HF-induced increase of tumor necrosis factor (TNF)-α. Emodin also affected the hepatocytes glutathione homeostasis and levels of the HFD/HF-induced increase of glutathionylated/phosphorylated phosphatase and tensin homolog (PTEN). In conclusion, we demonstrated that a natural agent such as emodin can prevent hepatosteatosis, preserving liver from pro-inflammatory and pro-oxidant damage caused by HFD/HF diet. These findings are promising, proposing emodin as a possible hindrance to progression of hepatosteatosis into steatohepatitis. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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26. Redox homeostasis and posttranslational modifications/activity of phosphatase and tensin homolog in hepatocytes from rats with diet-induced hepatosteatosis
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Alisi, Anna, Bruscalupi, Giovannella, Pastore, Anna, Petrini, Stefania, Panera, Nadia, Massimi, Mara, Tozzi, Giulia, Leoni, Silvia, Piemonte, Fiorella, and Nobili, Valerio
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HOMEOSTASIS , *POST-translational modification , *PHOSPHATASES , *OXIDATION-reduction reaction , *HOMOLOGY (Biology) , *LIVER cells , *FATTY liver , *LABORATORY rats - Abstract
Abstract: High-fat and high-carbohydrate diets may predispose to simple steatosis, alone or associated with necroinflammation and fibrosis (steatohepatitis). However, there are few reports about the real effect of these nutrients on hepatocyte redox homeostasis and consequent molecular derangement. Here, we investigated whether different diets would induce oxidative damage in primary rat hepatocytes and thereby affect the activity of phosphatase and tensin homolog (PTEN). We used Sprague–Dawley rats fed, for 14 weeks, a standard diet (SD), a high-fat/low-carbohydrate diet (HFD-LC), a normal-fat/high-fructose diet (NFD-HF), or a high-fat/high-fructose diet (HFD-HF). Metabolic and histological parameters were analyzed in blood and liver samples, while oxidative stress markers and related posttranscriptional modification of PTEN were analyzed in isolated hepatocytes. Our results indicate that different dietetic hypercaloric regimens caused liver damage and a significant increase of body and liver weight, as well as elevated plasma levels of alanine aminotransferase, triglycerides and insulin. Hepatocytes from NFD-HF and HFD-HF rats displayed a decrement of cell viability and proliferation rate. Hepatocytes from animals treated with hypercaloric regimens also exhibited oxidative stress greater than SD hepatocytes. Finally, NFD-HF and HFD-HF hepatocytes showed an increased PTEN phosphorylation and decreased PTEN activity, which seem strongly correlated to an increased glutathionylation of the protein. In conclusion, we demonstrate that fructose-enriched diets cause a tissue and hepatocyte damage that might exacerbate those observed in the presence of high-fat alone and might render, via redox homeostasis imbalance, the hepatocytes more prone to posttranslational modifications and activity alteration of PTEN. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
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