43 results on '"Luoping Zhang"'
Search Results
2. Comparison of hematological alterations and markers of B-cell activation in workers exposed to benzene, formaldehyde and trichloroethyl.
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A.Bassig, Bryan, Luoping Zhang, Vermeulen, Roel, Xiaojiang Tang, Guilan Li, Wei Hu, Weihong Guo, Purdue, Mark P., Songnian Yin, Rappaport, Stephen M., Min Shen, Zhiying Ji, Chuangyi Qiu, Yichen Ge, Hosgood, H. Dean, Reiss, Boris, Banghua Wu, Yuxuan Xie, Laiyu Li, and Fei Yue
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HEMATOLOGY , *TALL-1 (Protein) , *BENZENE , *FORMALDEHYDE , *TRICHLOROETHYLENE , *MYELOID leukemia - Abstract
Benzene, formaldehyde (FA) and trichloroethylene (TCE) are ubiquitous chemicals in workplaces and the general environment. Benzene is an established myeloid leukemogen and probable lymphomagen. FA is classified as a myeloid leukemogen but has not been associated with non-Hodgkin lymphoma (NHL), whereas TCE has been associated with NHL but not myeloid leukemia. Epidemiologic associations between FA and myeloid leukemia, and between benzene, TCE and NHL are, however, still debated. Previously, we showed that these chemicals are associated with hematotoxicity in cross-sectional studies of factory workers in China, which included extensive personal monitoring and biological sample collection. Here, we compare and contrast patterns of hematotoxicity, monosomy 7 in myeloid progenitor cells (MPCs), and B-cell activation biomarkers across these studies to further evaluate possible mechanisms of action and consistency of effects with observed hematologic cancer risks. Workers exposed to benzene or FA, but not TCE, showed declines in cell types derived from MPCs, including granulocytes and platelets. Alterations in lymphoid cell types, including B cells and CD4+ T cells, and B-cell activation markers were apparent in workers exposed to benzene or TCE. Given that alterations in myeloid and lymphoid cell types are associated with hematological malignancies, our data provide biologic insight into the epidemiological evidence linking benzene and FA exposure with myeloid leukemia risk, and TCE and benzene exposure with NHL risk. [ABSTRACT FROM AUTHOR]
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- 2016
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3. Parental, In Utero, and Early-Life Exposure to Benzene and the Risk of Childhood Leukemia: A Meta-Analysis.
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Carlos-Wallace, Frolayne M., Luoping Zhang, Smith, Martyn T., Rader, Gabriella, and Steinmaus, Craig
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LEUKEMIA risk factors , *CONFIDENCE intervals , *META-analysis , *RESEARCH funding , *ENVIRONMENTAL exposure , *DATA analysis software , *BENZENE derivatives , *DESCRIPTIVE statistics , *PRENATAL exposure delayed effects , *ODDS ratio , *CHILDREN , *FETUS - Abstract
Benzene is an established cause of adult leukemia, but whether it is associated with childhood leukemia remains unclear. We conducted a meta-analysis in which we reviewed the epidemiologic literature on this topic and explored causal inference, bias, and heterogeneity. The exposure metrics that we evaluated included occupational and household use of benzenes and solvents, traffic density, and traffic-related air pollution. For studies of occupational and household product exposure published from 1987 to 2014, the summary relative risk for childhood leukemia was 1.96 (95% confidence interval (CI): 1.53, 2.52; n = 20). In these studies, the summary relative risk was higher for acute myeloid leukemia (summary relative risk (sRR) = 2.34, 95% CI: 1.72, 3.18; n = 6) than for acute lymphoblastic leukemia (sRR= 1.57; 95% CI: 1.21,2.05; n= 14). The summary relative risk was higher for maternal versus paternal exposure, in studies that assessed benzene versus all solvents, and in studies of gestational exposure. In studies of traffic density or traffic-related air pollution published from 1999 to 2014, the summary relative risk was 1.48 (95% CI: 1.10,1.99; n = 12); it was higher for acute myeloid leukemia (sRR = 2.07; 95% CI: 1.34, 3.20) than for acute lymphoblastic leukemia (sRR = 1.49; 95% CI: 1.07,2.08) and in studies that involved detailed models of traffic pollution (sRR= 1.70; 95% CI: 1.16, 2.49). Overall, we identified evidence of associations between childhood leukemia and several different potential metrics of benzene exposure. [ABSTRACT FROM AUTHOR]
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- 2016
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4. Circulating immune/inflammation markers in Chinese workers occupationally exposed to formaldehyde.
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Wei Jie Seow, Luoping Zhang, Vermeulen, Roel, Xiaojiang Tang, Wei Hu, Bassig, Bryan A., Zhiying Ji, Shiels, Meredith S., Kemp, Troy J., Min Shen, Chuangyi Qiu, Reiss, Boris, Beane Freeman, Laura E., Blair, Aaron, Kim, Christopher, Weihong Guo, Cuiju Wen, Laiyu Li, Pinto, Ligia A., and Hanlin Huang
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GENETIC markers , *PHYSIOLOGICAL effects of formaldehyde , *MYELOID leukemia , *WORKING class , *CROSS-sectional method , *IMMUNOSUPPRESSION , *LEUKEMIA etiology - Abstract
Background. Formaldehyde has been classified as a human myeloid leukemogen. However, the mechanistic basis for this association is still debated. Objectives. We aimed to evaluate whether circulating immune/inflammation markers were altered in workers occupationally exposed to formaldehyde. Methods. Using a multiplexed bead-based assay, we measured serum levels of 38 immune/inflammation markers in a cross-sectional study of 43 formaldehyde-exposed and 51 unexposed factory workers in Guangdong, China. Linear regression models adjusting for potential confounders were used to compare marker levels in exposed and unexposed workers. Results. We found significantly lower circulating levels of two markers among exposed factory workers compared with unexposed controls that remained significant after adjusting for potential confounders and multiple comparisons using a false discovery rate of 10%, including chemokine (C-X-C motif) ligand 11 (36.2 pg/ml in exposed versus 48.4 pg/ml in controls, P = 0.0008) and thymus and activation regulated chemokine (52.7 pg/ml in exposed versus 75.0 pg/ml in controls, P = 0.0028), suggesting immunosuppression among formaldehyde-exposed workers. Conclusions. Our findings are consistent with recently emerging understanding that immunosuppression might be associated with myeloid diseases. These findings, if replicated in a larger study, may provide insights into the mechanisms by which formaldehyde promotes leukemogenesis. [ABSTRACT FROM AUTHOR]
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- 2015
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5. Water quality and non-point sources of risk: the Jiulong River Watershed, P. R. of China.
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Jingjing Zhang, Luoping Zhang, and Ricci, Paolo F.
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WATER quality , *NITROGEN in water , *FACTOR analysis , *TREND analysis , *MONTE Carlo method , *WATER pollution - Abstract
Retrospective water quality assessment plays an essential role in identifying trends and causal associations between exposures and risks, thus it can be a guide for water resources management. We have developed empirical relationships between several time-varying social and economic factors of economic development, water quality variables such as nitrate-nitrogen, CODMn, BOD5, and DO, in the Jiulong River Watershed and its main tributary, the West River. Our analyses used alternative statistical methods to reduce the dimensionality of the analysis first and then strengthen the study's causal associations. The statistical methods included: factor analysis (FA), trend analysis, Monte Carlo/bootstrap simulations, robust regressions and a coupled equations model, integrated into a framework that allows an investigation and resolution of the issues that may affect the estimated results. After resolving these, we found that the concentrations of nitrogen compounds increased over time in the west River region, and that fertilizer used in agricultural fruit crops was the main risk with regard to nitrogen pollution. The relationships we developed can identify hazards and explain the impact of sources of different types of pollution, such as urbanization, and agriculture. [ABSTRACT FROM AUTHOR]
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- 2012
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6. Global Gene Expression Profiling of a Population Exposed to a Range of Benzene Levels.
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McHale, Cliona M., Luoping Zhang, Qing Lan, Vermeulen, Roel, Guilan Li, Hubbard, Alan E., Porter, Kristin E., Thomas, Reuben, Portier, Christopher J., Min Shen, Rappaport, Stephen M., Songnian Yin, Smith, Martyn T., and Rothman, Nathaniel
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BENZENE , *BIOMARKERS , *COMPUTER software , *DOSE-effect relationship in pharmacology , *GENE expression , *GENETIC techniques , *INDOOR air pollution , *MOLECULAR epidemiology , *PATH analysis (Statistics) , *PROBABILITY theory , *RESEARCH funding , *STATISTICS , *OCCUPATIONAL hazards , *DATA analysis , *ENVIRONMENTAL exposure , *INTER-observer reliability , *CROSS-sectional method , *MICROARRAY technology - Abstract
Background: Benzene, an established cause of acute myeloid leukemia (AML), may also cause one or more lymphoid malignancies in humans. Previously, we identified genes and pathways associated with exposure to high (> 10 ppm) levels of benzene through transcriptomic analyses of blood cells from a small number of occupationally exposed workers. Objectives: The goals of this study were to identify potential biomarkers of benzene exposure and/or early effects and to elucidate mechanisms relevant to risk of hematotoxicity, leukemia, and lymphoid malignancy in occupationally exposed individuals, many of whom were exposed to benzene levels < 1 ppm, the current U.S. occupational standard. Methods: We analyzed global gene expression in the peripheral blood mononuclear cells of 125 workers exposed to benzene levels ranging from < 1 ppm to > 10 ppm. Study design and analysis with a mixed-effects model minimized potential confounding and experimental variability. Results: We observed highly significant widespread perturbation of gene expression at all exposure levels. The AML pathway was among the pathways most significantly associated with benzene exposure. Immune response pathways were associated with most exposure levels, potentially providing biological plausibility for an association between lymphoma and benzene exposure. We identified a 16-gene expression signature associated with all levels of benzene exposure. Conclusions: Our findings suggest that chronic benzene exposure, even at levels below the current U.S. occupational standard, perturbs many genes, biological processes, and pathways. These findings expand our understanding of the mechanisms by which benzene may induce hematotoxicity, leukemia, and lymphoma and reveal relevant potential biomarkers associated with a range of exposures. [ABSTRACT FROM AUTHOR]
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- 2011
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7. Paternal Smoking and Risk of Childhood Acute Lymphoblastic Leukemia: Systematic Review and Meta-Analysis.
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Ruiling Liu, Luoping Zhang, McHale, Cliona M., and Hammond, S. Katharine
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SMOKING , *LYMPHOBLASTIC leukemia , *EPIDEMIOLOGICAL research , *META-analysis , *SYSTEMATIC reviews - Abstract
Objective. To investigate the association between paternal smoking and childhood acute lymphoblastic leukemia (ALL). Method. We identified 18 published epidemiologic studies that reported data on both paternal smoking and childhood ALL risk. We performed a meta-analysis and analyzed dose-response relationships on ALL risk for smoking during preconception, during pregnancy, after birth, and ever smoking. Results. The summary odds ratio (OR) of childhood ALL associated with paternal smoking was 1.11 (95% Confidence Interval (CI): 1.05-1.18, I² = 18%) during any time period, 1.25 (95% CI: 1.08-1.46, I² = 53%) preconception; 1.24 (95% CI: 1.07-1.43, I² = 54%) during pregnancy, and 1.24 (95% CI: 0.96-1.60, I² = 64%) after birth, with a dose-response relationship between childhood ALL and paternal smoking preconception or after birth. Conclusion. The evidence supports a positive association between childhood ALL and paternal ever smoking and at each exposure time period examined. Future epidemiologic studies should assess paternal smoking during well-defined exposure windows and should include biomarkers to assess smoking exposure and toxicological mechanisms. [ABSTRACT FROM AUTHOR]
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- 2011
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8. Formaldehyde and Leukemia: An Updated Meta-Analysis and Evaluation of Bias.
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Schwilk, Erika, Luoping Zhang, Smith, Martyn T., Smith, Allan H., and Steinmaus, Craig
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ANALYSIS of variance , *COMPUTER software , *CONFIDENCE intervals , *DOSE-response relationship in biochemistry , *FORMALDEHYDE , *LEUKEMIA , *META-analysis , *PROBABILITY theory , *STATISTICS , *OCCUPATIONAL hazards , *DATA analysis , *ENVIRONMENTAL exposure , *RELATIVE medical risk , *DIAGNOSIS , *DRUG side effects - Abstract
The article presents a study on the relationship between formaldehyde and leukemia. It states that meta-analysis is used to evaluate the associative relation between the two factors. It mentions two groups of people involved in the study such as the less than 25,000 workers in formaldehyde industries in the U.S. and the less than 13,000 embalmers and funeral directors. Results of the study show that exposure to formaldehyde increased the risk of leukemia, particularly myeloid leukemia.
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- 2010
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9. Occupational exposure to trichloroethylene is associated with a decline in lymphocyte subsets and soluble CD27 and CD30 markers.
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Qing Lan, Luoping Zhang, Xiaojiang Tang, Min Shen, Smith, Martyn T., Chuangyi Qiu, Yichen Ge, Zhiying Ji, Jun Xiong, Jian He, Reiss, Boris, Zhenyue Hao, Songwang Liu, Yuxuan Xie, Weihong Guo, Purdue, Mark P., Galvan, Noe, Xin, Kerry X., Wei Hu, and Beane Freeman, Laura E.
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TRICHLOROETHYLENE , *LYMPHOMAS , *LYMPHOCYTE transformation , *CANCER genetics , *INDUSTRIAL hygiene , *EPIDEMIOLOGY - Abstract
Occupational cohort and case–control studies suggest that trichloroethylene (TCE) exposure may be associated with non-Hodgkin lymphoma (NHL) but findings are not consistent. There is a need for mechanistic studies to evaluate the biologic plausibility of this association. We carried out a cross-sectional molecular epidemiology study of 80 healthy workers that used TCE and 96 comparable unexposed controls in Guangdong, China. Personal exposure measurements were taken over a three-week period before blood collection. Ninety-six percent of workers were exposed to TCE below the current US Occupational Safety and Health Administration Permissible Exposure Limit (100 p.p.m. 8 h time-weighted average), with a mean (SD) of 22.2 (36.0) p.p.m. The total lymphocyte count and each of the major lymphocyte subsets including CD4+ T cells, CD8+ T cells, natural killer (NK) cells and B cells were significantly decreased among the TCE-exposed workers compared with controls (P < 0.05), with evidence of a dose-dependent decline. Further, there was a striking 61% decline in sCD27 plasma level and a 34% decline in sCD30 plasma level among TCE-exposed workers compared with controls. This is the first report that TCE exposure under the current Occupational Safety and Health Administration workplace standard is associated with a decline in all major lymphocyte subsets and sCD27 and sCD30, which play an important role in regulating cellular activity in subsets of T, B and NK cells and are associated with lymphocyte activation. Given that altered immunity is an established risk factor for NHL, these results add to the biologic plausibility that TCE is a possible lymphomagen. [ABSTRACT FROM PUBLISHER]
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- 2010
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10. The benzene metabolite, hydroquinone and etoposide both induce endoreduplication in human lymphoblastoid TK6 cells.
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Zhiying Ji, Luoping Zhang, Weihong Guo, McHale, Cliona M., and Smith, Martyn T.
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BENZENE , *FERTILIZATION in vitro , *HYDROQUINONE , *CHROMOSOMES , *DNA , *ETOPOSIDE , *CYTOGENETICS - Abstract
Both occupational exposure to the leukemogen benzene and in vitro exposure to its metabolite hydroquinone (HQ) lead to the induction of numerical and structural chromosome changes. Several studies have shown that HQ can form DNA adducts, disrupt microtubule assembly and inhibit DNA topoisomerase II (topo II) activity. As these are potential mechanisms underlying endoreduplication (END), a phenomenon that involves DNA amplification without corresponding cell division, we hypothesized that HQ could cause END. We measured END in the human lymphoblastoid cell line, TK6, treated with HQ (0–20 μM) and etoposide (0–0.2 μM) for 48 h. Etoposide was used as a positive control as it is a topo II poison and established human leukemogen that has previously been shown to induce END in Chinese hamster ovary cells. Both HQ and etoposide significantly induced END in a dose-dependent manner (Ptrend < 0.0001 and Ptrend = 0.0003, respectively). Since END may underlie the acquisition of high chromosome numbers by tumour cells, it may play a role in inducing genomic instability and subsequent carcinogenesis from HQ and etoposide. In order to further explore the cytogenetic effects of HQ and etoposide, we also examined specific structural changes. HQ did not induce translocations of chromosome 11 [t(11;?)] but significantly induced translocations of chromosome 21 [t(21;?)] and structural chromosome aberrations (SCA) (Ptrend = 0.0415 and Ptrend < 0.0001, respectively). Etoposide potently induced all these structural changes (Ptrend < 0.0001). The lack of an effect of HQ on t(11;?) and the reduced ability of HQ to induce t(21;?) and SCA, compared with etoposide, further suggests that HQ acts primarily as a topo II catalytic inhibitor rather than as a topo II poison in intact human cells. [ABSTRACT FROM PUBLISHER]
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- 2009
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11. The Nature of Chromosomal Aberrations Detected in Humans Exposed to Benzene.
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Luoping Zhang, Eastmond, David A., and Smith, Martyn T.
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BENZENE , *CHROMOSOME abnormalities , *TOXICOLOGY - Abstract
Deals with a study which examined the nature of chromosomal aberrations detected in humans exposed to benzene. Historical background; Discussion of chromosomal aberrations as a biomarker of cancer risk; Detection of chromosomal aberrations in preleukemia and benzene-poisoned patients; Cytogenetic changes detected in benzene-exposed individuals.
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- 2002
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12. High-resolution metabolomics of occupational exposure to trichloroethylene.
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Walker, Douglas I., Uppal, Karan, Luoping Zhang, Vermeulen, Roel, Smith, Martyn, Wei Hu, Purdue, Mark P., Xiaojiang Tang, Reiss, Boris, Sungkyoon Kim, Laiyu Li, Hanlin Huang, Pennell, Kurt D., Jones, Dean P., Rothman, Nathaniel, Qing Lan, Zhang, Luoping, Hu, Wei, Tang, Xiaojiang, and Kim, Sungkyoon
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METABOLOMICS , *PHYSIOLOGICAL effects of trichloroethylene , *INDUSTRIAL hygiene , *LYMPHOMAS , *RENAL cancer , *LIVER cancer , *HYDROCARBON metabolism , *BIOCHEMISTRY , *HYDROCARBONS , *KIDNEY tumors , *LIVER tumors , *MASS spectrometry , *METABOLISM , *OCCUPATIONAL diseases , *REGRESSION analysis , *RESEARCH funding , *OCCUPATIONAL hazards , *ENVIRONMENTAL exposure , *CROSS-sectional method , *CASE-control method - Abstract
Background: Occupational exposure to trichloroethylene (TCE) has been linked to adverse health outcomes including non-Hodgkin's lymphoma and kidney and liver cancer; however, TCE's mode of action for development of these diseases in humans is not well understood.Methods: Non-targeted metabolomics analysis of plasma obtained from 80 TCE-exposed workers [full shift exposure range of 0.4 to 230 parts-per-million of air (ppma)] and 95 matched controls were completed by ultra-high resolution mass spectrometry. Biological response to TCE exposure was determined using a metabolome-wide association study (MWAS) framework, with metabolic changes and plasma TCE metabolites evaluated by dose-response and pathway enrichment. Biological perturbations were then linked to immunological, renal and exposure molecular markers measured in the same population.Results: Metabolic features associated with TCE exposure included known TCE metabolites, unidentifiable chlorinated compounds and endogenous metabolites. Exposure resulted in a systemic response in endogenous metabolism, including disruption in purine catabolism and decreases in sulphur amino acid and bile acid biosynthesis pathways. Metabolite associations with TCE exposure included uric acid (β = 0.13, P-value = 3.6 × 10-5), glutamine (β = 0.08, P-value = 0.0013), cystine (β = 0.75, P-value = 0.0022), methylthioadenosine (β = -1.6, P-value = 0.0043), taurine (β = -2.4, P-value = 0.0011) and chenodeoxycholic acid (β = -1.3, P-value = 0.0039), which are consistent with known toxic effects of TCE, including immunosuppression, hepatotoxicity and nephrotoxicity. Correlation with additional exposure markers and physiological endpoints supported known disease associations.Conclusions: High-resolution metabolomics correlates measured occupational exposure to internal dose and metabolic response, providing insight into molecular mechanisms of exposure-related disease aetiology. [ABSTRACT FROM AUTHOR]- Published
- 2016
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13. Epigenome-wide association studies of occupational exposure to benzene and formaldehyde.
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Phillips, Rachael V., Linqing Wei, Cardenas, Andres, Hubbard, Alan E., McHale, Cliona M., Vermeulen, Roel, Hu Wei, Smith, Martyn T., Luoping Zhang, Qing Lan, and Rothman, Nathaniel
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- 2022
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14. Large-scale evaluation of candidate genes identifies associations between DNA repair and genomic maintenance and development of benzene hematotoxicity.
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Qing Lan, Luoping Zhang, Min Shen, William J. Jo, Roel Vermeulen, Guilan Li, Christopher Vulpe, Sophia Lim, Xuefeng Ren, Stephen M. Rappaport, Sonja I. Berndt, Meredith Yeager, Jeff Yuenger, Richard B. Hayes, Martha Linet, Songnian Yin, Stephen Chanock, Martyn T. Smith, and Nathaniel Rothman
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DNA repair , *LEUKEMIA etiology , *GENETIC polymorphisms , *BENZENE in the body , *TOXICOLOGY , *DISEASE susceptibility , *GENOTYPE-environment interaction , *GENETIC recombination - Abstract
Benzene is an established human hematotoxicant and leukemogen but its mechanism of action is unclear. To investigate the role of single-nucleotide polymorphisms (SNPs) on benzene-induced hematotoxicity, we analyzed 1395 SNPs in 411 genes using an Illumina GoldenGate assay in 250 benzene-exposed workers and 140 unexposed controls. Highly significant findings clustered in five genes (BLM, TP53, RAD51, WDR79 and WRN) that play a critical role in DNA repair and genomic maintenance, and these regions were then further investigated with tagSNPs. One or more SNPs in each gene were associated with highly significant 10–20% reductions (P values ranged from 0.0011 to 0.0002) in the white blood cell (WBC) count among benzene-exposed workers but not controls, with evidence for gene–environment interactions for SNPs in BLM, WRN and RAD51. Further, among workers exposed to benzene, the genotype-associated risk of having a WBC count <4000 cells/μl increased when using individuals with progressively higher WBC counts as the comparison group, with some odds ratios >8-fold. In vitro functional studies revealed that deletion of SGS1 in yeast, equivalent to lacking BLM and WRN function in humans, caused reduced cellular growth in the presence of the toxic benzene metabolite hydroquinone, and knockdown of WRN using specific short hairpin RNA increased susceptibility of human TK6 cells to hydroquinone toxicity. Our findings suggest that SNPs involved in DNA repair and genomic maintenance, with particular clustering in the homologous DNA recombination pathway, play an important role in benzene-induced hematotoxicity. [ABSTRACT FROM AUTHOR]
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- 2009
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15. Consensus on the Key Characteristics of Immunotoxic Agents as a Basis for Hazard Identification.
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Germolec, Dori R., Lebrec, Herve, Anderson, Stacey E., Burleson, Gary R., Cardenas, Andres, Corsini, Emanuela, Elmore, Sarah E., Kaplan, Barbara L. F., Lawrence, B. Paige, Lehmann, Geniece M., Maier, Curtis C., McHale, Cliona M., Myers, L. Peyton, Pallardy, Marc, Rooney, Andrew A., Zeise, Lauren, Luoping Zhang, and Smith, Martyn T.
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CONSENSUS (Social sciences) , *CELL differentiation , *CARCINOGENS , *COMMITTEES , *HAZARDOUS substances , *MEDICAL personnel , *APOPTOSIS , *IMMUNE system , *IMMUNOSUPPRESSION , *RISK assessment , *CELLULAR signal transduction , *CELL communication , *EXPERTISE , *CELL proliferation , *IMMUNITY , *CELL surface antigens , *BIOLOGICAL assay , *IMMUNODIAGNOSIS , *ANTIGENS - Abstract
BACKGROUND: Key characteristics (KCs), properties of agents or exposures that confer potential hazard, have been developed for carcinogens and other toxicant classes. KCs have been used in the systematic assessment of hazards and to identify assay and data gaps that limit screening and risk assessment. Many of the mechanisms through which pharmaceuticals and occupational or environmental agents modulate immune function are well recognized. Thus KCs could be identified for immunoactive substances and applied to improve hazard assessment of immunodulatory agents. OBJECTIVES: The goal was to generate a consensus-based synthesis of scientific evidence describing the KCs of agents known to cause immunotoxicity and potential applications, such as assays to measure the KCs. METHODS: A committee of 18 experts with diverse specialties identified 10 KCs of immunotoxic agents, namely, 1) covalently binds to proteins to form novel antigens, 2) affects antigen processing and presentation, 3) alters immune cell signaling, 4) alters immune cell proliferation, 5) modifies cellular differentiation, 6) alters immune cell–cell communication, 7) alters effector function of specific cell types, 8) alters immune cell trafficking, 9) alters cell death processes, and 10) breaks down immune tolerance. The group considered how these KCs could influence immune processes and contribute to hypersensitivity, inappropriate enhancement, immunosuppression, or autoimmunity. DISCUSSION: KCs can be used to improve efforts to identify agents that cause immunotoxicity via one or more mechanisms, to develop better testing and biomarker approaches to evaluate immunotoxicity, and to enable a more comprehensive and mechanistic understanding of adverse effects of exposures on the immune system. [ABSTRACT FROM AUTHOR]
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- 2022
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16. Linking the concept of ecological footprint and valuation of ecosystem services: A case study of economic growth and natural carrying capacity.
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Sheng Zhao, Huasheng Hong, and Luoping Zhang
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SUSTAINABLE development , *ECONOMIC development & the environment , *ENVIRONMENTAL economics , *RENEWABLE natural resources , *INDUSTRIALIZATION & the environment , *ENVIRONMENTAL protection - Abstract
Human activities have become so extensive that all ecosystems on the planet have been altered to some extent. The fate of humankind will be determined by how sustainable ecosystems and the renewable resources in them are managed. The implication of this is obvious: humanity must live within nature's carrying capacity. In recent years, humans have recognised that growth of the economy depends on natural capital, and it is important that we now recognise that we are part of an international ecological economics community, so as to better integrate the economy and ecology. However, there are few successful examples of this. The aim of this paper is to show a method for integrated analysis between economic growth and natural carrying capacity by linking the concepts of ecological footprint and valuation of ecosystem services. When applied to China for the period 1987-2003, the empirical evidence suggests that the size of the Chinese economy surpassed the carrying capacity in 1992. Perhaps, we should abandon our high-growth predilection and initiate a transition to a steady-state economy. [ABSTRACT FROM AUTHOR]
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- 2008
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17. Nitrogen sources and exports in an agricultural watershed in Southeast China.
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Nengwang Chen, Huasheng Hong, Luoping Zhang, and Wenzhi Cao
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NITROGEN , *WATERSHEDS , *AGRICULTURE , *SURVEYS , *FLUX (Metallurgy) , *VAPORIZATION in water purification , *BIOGEOCHEMISTRY , *ATMOSPHERIC deposition - Abstract
The nitrogen (N) budget was developed for Jiulong River Watershed (JRW), an agricultural watershed in a warm and humid area of southeast China. Water quality monitoring, field surveys, modelling and GIS techniques were applied to estimate N flux of atmospheric deposition, mineralization, runoff, denitrification, and ammonia volatilization. Over the whole watershed, fertilizers, import of animal feeds, biotic fixation, mineralization and atmospheric deposition contributed 67.1%, 16.5%, 2.1%, 4.9% and 9.5%, respectively, of total N input (129.3 kg N ha−1 year−1). Runoff, sale of production, denitrification, and ammonia volatilization contributed 7.3%, 24.4%, 10.5% and 57.8% of total N output (72.9 kg N ha−1 year−1), respectively. The N budget for the JRW suggested that more than 50% of the N input was lost to the environment, and about 14% was discharged as riverine N, which indicated that agricultural and human activities in the watershed substantially impacted the estuary and coastal water quality, and so altered the N biogeochemistry process. [ABSTRACT FROM AUTHOR]
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- 2008
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18. Decreased levels of CXC-chemokines in serum of benzene-exposed workers identified by array-based proteomics.
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Vermeulen, Roel, Qing Lan, Luoping Zhang, Gunn, Laura, McCarthy, Diane, Woodbury, Ronald L., McGuire, Marielena, Podust, Vladimir N., Li, Guilan, Chatterjee, Nilanjan, Ruidong Mu, Songnian Yin, Rothman, Nathaniel, and Smith, Martyn T.
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AROMATIC compounds , *CHEMOKINES , *IMMUNOSUPPRESSIVE agents , *BIOCHEMISTRY , *PROTEOMICS , *IONIZATION (Atomic physics) - Abstract
Benzene is an important industrial chemical and environmental contaminant that causes leukemia. To obtain mechanistic insight into benzene's mechanism of action, we examined the impact of benzene on the human serum proteome in a study of exposed healthy shoe-factory workers and unexposed controls. Two sequential studies were performed, each using sera from 10 workers exposed to benzene (overall mean benzene air level >30 ppm) and 10 controls. Serum samples were subjected to anion-exchange fractionation and bound to three types of ProteinChip arrays (Ciphergen Biosystems, Fremont, CA) (hydrophobic (H50), metal affinity (IMAC3-Cu), and cation exchange (WCX2)]. Protein-expression patterns were detected by surface-enhanced laser desorption/ ionization (SELDI)-TOF MS. Three proteins (4.1, 7.7, and 9.3 kDa) were consistently down-regulated in exposed compared with control subjects in both studies. All proteins were highly inversely correlated with individual estimates of benzene exposure (r > 0.75). The 7.7- and 9.3-kDa proteins were subsequently identified as platelet factor (PF)4 and connective tissue activating peptide (CTAP)-Ill. Initial proteomic results for PF4 and CTAP-Ill were subsequently confirmed in a single experiment using a ProteinChip- array-based immunoassay(Ciphergen Biosystems). The altered expression of the platelet-derived CXC-chemokines (40% and 63% for PF4 and GAP-Ill, respectively) could not be explained by changes in absolute platelet counts. Thus, SELDI-TOF analysis of a limited number of exposed and unexposed subjects revealed that lowered expression of PF4 and GAP-Ill proteins is a potential biomarker of benzene's early biologic effects and may play a role in the immunosuppressive effects of benzene. [ABSTRACT FROM AUTHOR]
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- 2005
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19. Using the Key Characteristics of Carcinogens to Develop Research on Chemical Mixtures and Cancer.
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Rider, Cynthia V., McHale, Cliona M., Webster, Thomas F., Lowe, Leroy, Goodson III, William H., La Merrill, Michele A., Rice, Glenn, Zeise, Lauren, Luoping Zhang, and Smith, Martyn T.
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TUMOR risk factors , *CARCINOGENS , *RISK assessment , *HUMAN services programs , *MEDICAL research , *ENVIRONMENTAL exposure - Abstract
BACKGROUND: People are exposed to numerous chemicals throughout their lifetimes. Many of these chemicals display one or more of the key characteristics of carcinogens or interact with processes described in the hallmarks of cancer. Therefore, evaluating the effects of chemical mixtures on cancer development is an important pursuit. Challenges involved in designing research studies to evaluate the joint action of chemicals on cancer risk include the time taken to perform the experiments because of the long latency and choosing an appropriate experimental design. OBJECTIVES: The objectives of this work are to present the case for developing a research program on mixtures of environmental chemicals and cancer risk and describe recommended approaches. METHODS: A working group comprising the coauthors focused attention on the design of mixtures studies to inform cancer risk assessment as part of a larger effort to refine the key characteristics of carcinogens and explore their application. Working group members reviewed the key characteristics of carcinogens, hallmarks of cancer, and mixtures research for other disease end points. The group discussed options for developing tractable projects to evaluate the joint effects of environmental chemicals on cancer development. RESULTS AND DISCUSSION: Three approaches for developing a research program to evaluate the effects of mixtures on cancer development were proposed: a chemical screening approach, a transgenic model-based approach, and a disease-centered approach. Advantages and disadvantages of each are discussed. [ABSTRACT FROM AUTHOR]
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- 2021
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20. Proposed Key Characteristics of Female Reproductive Toxicants as an Approach for Organizing and Evaluating Mechanistic Data in Hazard Assessment.
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Luderer, Ulrike, Eskenazi, Brenda, Hauser, Russ, Korach, Kenneth S., McHale, Cliona M., Moran, Francisco, Rieswijk, Linda, Solomon, Gina, Udagawa, Osamu, Luoping Zhang, Zlatnik, Marya, Zeise, Lauren, and Smith, Martyn T.
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CELL receptors , *ANIMAL experimentation , *CELL physiology , *CELLULAR signal transduction , *DATABASE management , *DIETHYLSTILBESTROL , *DIOXINS , *DISCUSSION , *FEMALE reproductive organs , *GENES , *GENITOURINARY organs , *HAZARDOUS substances , *HORMONES , *IMMUNE system , *MEETINGS , *MICE , *MITOCHONDRIA , *GENETIC mutation , *RESEARCH funding , *RISK assessment , *UNIVERSITIES & colleges , *REPRODUCTIVE health , *ENVIRONMENTAL exposure , *OXIDATIVE stress , *CYCLOPHOSPHAMIDE , *EPIGENOMICS - Abstract
BACKGROUND: Identification of female reproductive toxicants is currently based largely on integrated epidemiological and in vivo toxicology data and, to a lesser degree, on mechanistic data. A uniform approach to systematically search, organize, integrate, and evaluate mechanistic evidence of female reproductive toxicity from various data types is lacking. OBJECTIVE: We sought to apply a key characteristics approach similar to that pioneered for carcinogen hazard identification to female reproductive toxicant hazard identification. METHODS: A working group of international experts was convened to discuss mechanisms associated with chemical-induced female reproductive toxicity and identified 10 key characteristics of chemicals that cause female reproductive toxicity: 1) alters hormone receptor signaling; alters reproductive hormone production, secretion, or metabolism; 2) chemical or metabolite is genotoxic; 3) induces epigenetic alterations; 4) causes mitochondrial dysfunction; 5) induces oxidative stress; 6) alters immune function; 7) alters cell signal transduction; 8) alters direct cell-cell interactions; 9) alters survival, proliferation, cell death, or metabolic pathways; and 10) alters microtubules and associated structures. As proof of principle, cyclophosphamide and diethylstilbestrol (DES), for which both human and animal studies have demonstrated female reproductive toxicity, display at least 5 and 3 key characteristics, respectively. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), for which the epidemiological evidence is mixed, exhibits 5 key characteristics. DISCUSSION: Future efforts should focus on evaluating the proposed key characteristics against additional known and suspected female reproductive toxicants. Chemicals that exhibit one or more of the key characteristics could be prioritized for additional evaluation and testing. A key characteristics approach has the potential to integrate with pathway-based toxicity testing to improve prediction of female reproductive toxicity in chemicals and potentially prevent some toxicants from entering common use. [ABSTRACT FROM AUTHOR]
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- 2019
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21. Adductomic signatures of benzene exposure provide insights into cancer induction.
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Grigoryan, Hasmik, Edmands, William M. B., Qing Lan, Carlsson, Henrik, Vermeulen, Roel, Luoping Zhang, Song-Nian Yin, Gui-Lan Li, Smith, Martyn T., Rothman, Nathaniel, and Rappaport, Stephen M.
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BENZENE , *LEUKEMIA risk factors , *METABOLISM , *CHEMICAL adducts - Abstract
Although benzene has long been recognized as a cause of human leukemia, the mechanism by which this simple molecule causes cancer has been problematic. A complicating factor is benzene metabolism, which produces many reactive intermediates, some specific to benzene and others derived from redox processes. Using archived serum from 20 nonsmoking Chinese workers, 10 with and 10 without occupational exposure to benzene (exposed: 3.2-88.9 ppm, controls: 0.002-0.020 ppm), we employed an adductomic pipeline to characterize protein modifications at Cys34 of human serum albumin, a nucleophilic hotspot in extracellular fluids. Of the 47 measured human serum albumin modifications, 39 were present at higher concentrations in benzene-exposed workers than in controls and many of the exposed-control differences were statistically significant. Correlation analysis identified three prominent clusters of adducts, namely putative modifications by benzene oxide and a benzene diolepoxide that grouped with other measures of benzene exposure, adducts of reactive oxygen and carbonyl species, and Cys34 disulfides of small thiols that are formed following oxidation of Cys34. Benzene diolepoxides are potent mutagens and carcinogens that have received little attention as potential causes of human leukemia. Reactive oxygen and carbonyl species--generated by redox processes involving polyphenolic benzene metabolites and by Cyp2E1 regulation following benzene exposure--can modify DNA and proteins in ways that contribute to cancer. The fact that these diverse human serum albumin modifications differed between benzene-exposed and control workers suggests that benzene can increase leukemia risks via multiple pathways involving a constellation of reactive molecules. [ABSTRACT FROM AUTHOR]
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- 2018
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22. High-Throughput Functional Genomics Identifies Modulators of TCE Metabolite Genotoxicity and Candidate Susceptibility Genes.
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De La Rosa, Vanessa Y., Asfaha, Jonathan, Fasullo, Michael, Loguinov, Alex, Peng Li, Moore, Lee E., Rothman, Nathaniel, Nakamura, Jun, Swenberg, James A., Scelo, Ghislaine, Luoping Zhang, Smith, Martyn T., and Vulpe, Chris D.
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DNA repair , *GENOME editing , *EUKARYOTES , *MUTAGENICITY testing , *CHRONIC toxicity testing - Abstract
Trichloroethylene (TCE), an industrial chemical and environmental contaminant, is a human carcinogen. Reactive metabolites are implicated in renal carcinogenesis associated with TCE exposure, yet the toxicity mechanisms of these metabolites and their contribution to cancer and other adverse effects remain unclear. We employed an integrated functional genomics approach that combined functional profiling studies in yeast and avian DT40 cell models to provide new insights into the specific mechanisms contributing to toxicity associated with TCE metabolites. Genome-wide profiling studies in yeast identified the error-prone translesion synthesis (TLS) pathway as an import mechanism in response to TCE metabolites. The role of TLS DNA repair was further confirmed by functional profiling in DT40 avian cell lines, but also revealed that TLS and homologous recombination DNA repair likely play competing roles in cellular susceptibility to TCE metabolites in higher eukaryotes. These DNA repair pathways are highly conserved between yeast, DT40, and humans. We propose that in humans, mutagenic TLS is favored over homologous recombination repair in response to TCE metabolites. The results of these studies contribute to the body of evidence supporting a mutagenic mode of action for TCE-induced renal carcinogenesis mediated by reactive metabolites in humans. Our approach illustrates the potential for highthroughput in vitro functional profiling in yeast to elucidate toxicity pathways (molecular initiating events, key events) and candidate susceptibility genes for focused study. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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23. Interactive Influence of N6AMT1 and As3MT Genetic Variations on Arsenic Metabolism in the Population of Inner Mongolia, China.
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Xushen Chen, Xiaojuan Guo, Ping He, Jing Nie, Xiaoyan Yan, Jinqiu Zhu, Luoping Zhang, Guangyun Mao, Hongmei Wu, Zhiyue Liu, Aga, Diana, Peilin Xu, Smith, Martyn, and Xuefeng Ren
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HUMAN genetic variation , *ARSENIC metabolism , *CACODYLIC acid , *DNA methyltransferases , *BIOMETHYLATION - Abstract
Chronic arsenic exposure via drinking water has become a worldwide public health concern. In humans, inorganic arsenic (iAs) is metabolized to monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) mainly mediated by arsenic (þ3 oxidation state) methyltransferase (As3MT). We reported recently that N-6 adenine-specific DNA methyltransferase 1 (N6AMT1) was involved in arsenic metabolism, and examined its interactive effect with As3MT on arsenic metabolism in vitro. To further evaluate the interactive effect of N6AMT1 and As3MT on arsenic biomethylation in humans, we conducted a human population-based study including 289 subjects living in rural villages in Inner Mongolia, China, and assessed their urinary arsenic metabolites profiles in relation to genetic polymorphisms and haplotypes of N6AMT1 and As3MT. Five N6AMT1 single nucleotide polymorphisms (SNPs; rs1003671, rs7282257, rs2065266, rs2738966, rs2248501) and the N6AMT1 haplotype 2_GGCCAT were significantly associated with the percentage of iAs (% iAs) in urine (e.g., for rs7282257, mean was 9.62% for TT, 6.73% for AA). Rs1003671 was also in a significant relationship with urinary MMA and DMA (the mean of %MMA was 24.95% for GA, 31.69% for GG; the mean of % DMA was 69.21% for GA, 59.82% for GG). The combined effect of N6AMT1 haplotype 2_GGCCAT and As3MT haplotype 2_GCAC showed consistence with the additive significance of each haplotype on % iAs: the mean was 5.47% and 9.36% for carriers with both and null haplotypes, respectively. Overall, we showed that N6AMT1 genetic polymorphisms were associated with arsenic biomethylation in the Chinese population, and its interaction with As3MT was observed in specific haplotype combinations. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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24. Response.
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Qing Lan, Vermeulen, Roel, Luoping Zhang, Guilan Li, Rosenberg, Philip S., Alter, Blanche P., Min Shen, Rappaport, Stephen M., Weinberg, Rona S., Chanock, Stephen, Waidyanatha, Suramya, Rabkin, Charles, Hayes, Richard B., Linet, Martha, Sungkyoon Kim, Songnian Yin, Rothman, Nathaniel, and Smith, Martyn T.
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LETTERS to the editor , *BENZENE - Abstract
A response by Qing Lan to a letter to the editor about his article "Hematotoxicity in Workers exposed to low levels of benzene," in the December 3, 2004 issue is presented.
- Published
- 2006
25. Identification of Genes That Modulate Susceptibility to Formaldehyde and Imatinib by Functional Genomic Screening in Human Haploid KBM7 Cells.
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Hua Shen, McHale, Cliona M., Haider, Syed I., Cham Jung, Susie Zhang, Smith, Martyn T., and Luoping Zhang
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CHRONIC myeloid leukemia , *FORMALDEHYDE , *GENETICS of disease susceptibility , *IMATINIB , *FUNCTIONAL genomics , *GENETIC testing - Abstract
Though current functional genomic screening systems are useful for investigating human susceptibility to chemical toxicity, they have limitations. Well-established, high-throughput yeast mutant screens identify only evolutionarily conserved processes. RNA interference can be applied in human cells but is limited by incomplete gene knockout and off-target effects. Human haploid cell screening is advantageous as it requires knockdown of only a single copy of each gene. A human haploid cell mutant library (KBM7-Mu), derived from a chronic myeloid leukemia (CML) patient, was recently developed and has been used to identify genes that modulate sensitivity to infectious agents and pharmaceutical drugs. Here, we sought to improve the KBM7-Mu screening process to enable efficient screening of environmental chemicals. We developed a semi-solid medium based screening approach that cultures individual mutant colonies from chemically resistant cells, faster (by 2-3 weeks) and with less labor than the original liquid medium-based approach. As proof of principle, we identified genetic mutants that confer resistance to the carcinogen formaldehyde (FA, 12 genes, 18 hits) and the CML chemotherapeutic agent imatinib (6 genes, 13 hits). Validation experiments conducted on KBM7 mutants lacking each of the 18 genes confirmed resistance of 6 FA mutants (CTC1, FCRLA, GOT1, LPR5, M1AP, and MAP2K5) and 1 imatinib-resistant mutant (LYRM9). Despite the improvements to the method, it remains technically challenging to limit false positive findings. Nonetheless, our findings demonstrate the broad applicability of this optimized haploid approach to screen toxic chemicals to identify novel susceptibility genes and gain insight into potential mechanisms of toxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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26. Response to letter to the editor of Carcinogenesis by Pira et al., 2017.
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Rothman, Nathaniel, Qing Lan, Smith, Martyn T., Vermeulen, Roel, and Luoping Zhang
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PROGENITOR cells , *CHROMOSOMES - Published
- 2017
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27. Modulation of Ras signaling alters the toxicity of hydroquinone, a benzene metabolite and component of cigarette smoke.
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North, Matthew, Shuga, Joe, Fromowitz, Michele, Loguinov, Alexandre, Shannon, Kevin, Luoping Zhang, Smith, Martyn T., and Vulpe, Chris D.
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HYDROQUINONE , *CIGARETTE smoke , *SACCHAROMYCES cerevisiae , *TUMOR suppressor genes , *NEUROFIBROMIN , *PROGENITOR cells - Abstract
Background Benzene is an established human leukemogen, with a ubiquitous environmental presence leading to significant population exposure. In a genome-wide functional screen in the yeast Saccharomyces cerevisiae, inactivation of IRA2, a yeast ortholog of the human tumor suppressor gene NF1 (Neurofibromin), enhanced sensitivity to hydroquinone, an important benzene metabolite. Increased Ras signaling is implicated as a causal factor in the increased pre-disposition to leukemia of individuals with mutations in NF1. Methods Growth inhibition of yeast by hydroquinone was assessed in mutant strains exhibiting varying levels of Ras activity. Subsequently, effects of hydroquinone on both genotoxicity (measured by micronucleus formation) and proliferation of WT and Nf1 null murine hematopoietic precursors were assessed. Results Here we show that the Ras status of both yeast and mammalian cells modulates hydroquinone toxicity, indicating potential synergy between Ras signaling and benzene toxicity. Specifically, enhanced Ras signaling increases both hydroquinone-mediated growth inhibition in yeast and genotoxicity in mammalian hematopoetic precursors as measured by an in vitro erythroid micronucleus assay. Hydroquinone also increases proliferation of CFUGM progenitor cells in mice with Nf1 null bone marrow relative to WT, the same cell type associated with benzene-associated leukemia. Conclusions Together our findings show that hydroquinone toxicity is modulated by Ras signaling. Individuals with abnormal Ras signaling could be more vulnerable to developing myeloid diseases after exposure to benzene. We note that hydroquinone is used cosmetically as a skinbleaching agent, including by individuals with cafe-au-lait spots (which may be present in individuals with neurofibromatosis who have a mutation in NF1), which could be unadvisable given our findings. [ABSTRACT FROM AUTHOR]
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- 2014
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28. Species diversity patterns of marine plankton and benthos in Chinese bays: Baseline prior to large-scale development.
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Weiwei Yu, Bin Chen, Ricci, Paolo F., Luoping Zhang, Jianguo Du, and Zhiyuan Ma
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PLANKTON , *AQUATIC organisms , *AQUATIC biology , *BIOLOGICAL classification , *GENETICS - Abstract
More than 28,000 marine species have been recorded in China, which accounts for approximately 10% of all marine organisms in the world and plays a potentially important role in protecting global marine biodiversity. However, knowledge of marine biodiversity patterns in China is limited, and in particular, no comparative diversity analysis has been carried out for Chinese bays. In this study, national-scale species diversity patterns of coastal bays were examined on the basis of investigations for approximately 81 bays throughout the entire Chinese coastline in the 1980s and the early 1990s, revealing the baseline of diversity patterns prior to large-scale development. Diversity patterns found for coastal bays in China in this study include the following: (1) species richness of benthic macrofauna was larger than that of phytoplankton or zooplankton; (2) spatially, species richness in the subtropical zone was significantly greater than that in the temperate zone; (3) species richness and bay area were significantly correlated and followed power law relationships; and (4) there were significantly positive correlations of species richness among phytoplankton, zooplankton, and benthic macrofauna. The species diversity patterns of marine benthos and plankton for coastal bays in China, in some ways, coincided with general terrestrial patterns. This is the first study to examine national-scale species diversity patterns of coastal bays in China. The findings provide new insights to conservation biology in the marine environment and also are fundamental for future studies of biodiversity and the impact of development on biodiversity. [ABSTRACT FROM AUTHOR]
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- 2015
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29. Effect of Chemical Mutagens and Carcinogens on Gene Expression Profiles in Human TK6 Cells.
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Godderis, Lode, Thomas, Reuben, Hubbard, Alan E., Tabish, Ali M., Hoet, Peter, Luoping Zhang, Smith, Martyn T., Veulemans, Hendrik, and McHale, Cliona M.
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CARCINOGENS , *MUTAGENS , *GENE expression , *CELLS , *RNA , *ETHYLENE - Abstract
Characterization of toxicogenomic signatures of carcinogen exposure holds significant promise for mechanistic and predictive toxicology. In vitro transcriptomic studies allow the comparison of the response to chemicals with diverse mode of actions under controlled experimental conditions. We conducted an in vitro study in TK6 cells to characterize gene expression signatures of exposure to 15 genotoxic carcinogens frequently used in European industries. We also examined the dose-responsive changes in gene expression, and perturbation of biochemical pathways in response to these carcinogens. TK6 cells were exposed at 3 dose levels for 24 h with and without S9 human metabolic mix. Since S9 had an impact on gene expression (885 genes), we analyzed the gene expression data from cells cultures incubated with S9 and without S9 independently. The ribosome pathway was affected by all chemical-dose combinations. However in general, no similar gene expression was observed among carcinogens. Further, pathways, i.e. cell cycle, DNA repair mechanisms, RNA degradation, that were common within sets of chemical-dose combination were suggested by clustergram. Linear trends in dose-response of gene expression were observed for Trichloroethylene, Benz[a]anthracene, Epichlorohydrin, Benzene, and Hydroquinone. The significantly altered genes were involved in the regulation of (anti-) apoptosis, maintenance of cell survival, tumor necrosis factor-related pathways and immune response, in agreement with several other studies. Similarly in S9+ cultures, Benz[a]pyrene, Styrene and Trichloroethylene each modified over 1000 genes at high concentrations. Our findings expand our understanding of the transcriptomic response to genotoxic carcinogens, revealing the alteration of diverse sets of genes and pathways involved in cellular homeostasis and cell cycle control. [ABSTRACT FROM AUTHOR]
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- 2012
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30. Comparison of Proliferation and Genomic Instability Responses to WRN Silencing in Hematopoietic HL60 and TK6 Cells.
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Xuefeng Ren, Sophia Lim, Zhiying Ji, Jessica Yuh, Vivian Peng, Smith, Martyn T., and Luoping Zhang
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WERNER'S syndrome , *DNA helicases , *CARCINOGENESIS , *FIBROBLASTS , *CELL proliferation , *DNA damage , *CELL cycle , *CELL culture , *HEREDITY - Abstract
Background: Werner syndrome (WS) results from defects in the RecQ helicase (WRN) and is characterized by premature aging and accelerated tumorigenesis. Contradictorily, WRN deficient human fibroblasts derived from WS patients show a characteristically slower cell proliferation rate, as do primary fibroblasts and human cancer cell lines with WRN depletion. Previous studies reported that WRN silencing in combination with deficiency in other genes led to significantly accelerated cellular proliferation and tumorigenesis. The aim of the present study was to examine the effects of silencing WRN in p53 deficient HL60 and p53 wild-type TK6 hematopoietic cells, in order to further the understanding of WRN-associated tumorigenesis. Methodology/Principal Findings: We found that silencing WRN accelerated the proliferation of HL60 cells and decreased the cell growth rate of TK6 cells. Loss of WRN increased DNA damage in both cell types as measured by COMET assay, but elicited different responses in each cell line. In HL60 cells, but not in TK6 cells, the loss of WRN led to significant increases in levels of phosphorylated RB and numbers of cells progressing from G1 phase to S phase as shown by cell cycle analysis. Moreover, WRN depletion in HL60 cells led to the hyper-activation of homologous recombination repair via up-regulation of RAD51 and BLM protein levels. This resulted in DNA damage disrepair, apparent by the increased frequencies of both spontaneous and chemically induced structural chromosomal aberrations and sister chromatid exchanges. Conclusions/Significance: Together, our data suggest that the effects of WRN silencing on cell proliferation and genomic instability are modulated probably by other genetic factors, including p53, which might play a role in the carcinogenesis induced by WRN deficiency. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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31. An Emerging Role for Epigenetic Dysregulation in Arsenic Toxicity and Carcinogenesis.
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Xuefeng Ren, McHale, Cliona M., Skibola, Christine F., Smith, Allan H., Smith, Martyn T., and Luoping Zhang
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ARSENIC , *ARSENIC poisoning , *CARCINOGENS , *DNA , *GENE expression , *METHYLATION , *RNA , *TUMORS , *WATER supply , *ENVIRONMENTAL exposure , *PHARMACODYNAMICS - Abstract
Background: Exposure to arsenic, an established human carcinogen, through consumption of highly contaminated drinking water is a worldwide public health concern. Several mechanisms by which arsenical compounds induce tumorigenesis have been proposed, including oxidative stress, genotoxic damage, and chromosomal abnormalities. Recent studies have suggested that epigenetic mechanisms may also mediate toxicity and carcinogenicity resulting from arsenic exposure. Objective: We examined the evidence supporting the roles of the three major epigenetic mechanisms--DNA methylation, histone modification, and microRNA (miRNA) expression--in arsenic toxicity and, in particular, carcinogenicity. We also investigated future research directions necessary to clarify epigenetic and other mechanisms in humans. Data sources and synthesis: We conducted a PubMed search of arsenic exposure and epigenetic modification through April 2010 and summarized the in vitro and in vivo research findings, from both our group and others, on arsenic-associated epigenetic alteration and its potential role in toxicity and carcinogenicity. Conclusions: Arsenic exposure has been shown to alter methylation levels of both global DNA and gene promoters; histone acetylation, methylation, and phosphorylation; and miRNA expression, in studies analyzing mainly a limited number of epigenetic end points. Systematic epigenomic studies in human populations exposed to arsenic or in patients with arsenic-associated cancer have not yet been performed. Such studies would help to elucidate the relationship between arsenic exposure, epigenetic dysregulation, and carcinogenesis and are becoming feasible because of recent technological advancements. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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32. Benzene Exposure Near the U.S. Permissible Limit Is Associated with Sperm Aneuploidy.
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Caihong Xing, Marchetti, Francesco, Guilan Li, Weldon, Rosana H., Kurtovich, Elaine, Young, Suzanne, Schmid, Thomas E., Luoping Zhang, Rappaport, Stephen, Suramya Waidyanatha, Wyrobek, Andrew J., and Eskenazi, Brenda
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BENZENE , *ANEUPLOIDY , *FLUORESCENCE in situ hybridization , *LEUKEMIA , *BLOOD cells , *VENOUS puncture , *SPERMATOZOA , *IONIZATION (Atomic physics) , *GAS chromatography , *DISEASES - Abstract
Background: Benzene is a common industrial chemical known to induce leukemia and other blood disorders, as well as aneuploidy, in both human blood cells and sperm at exposures > 10 ppm. Recent reports have identified health effects at exposure levels < 1 ppm, the permissible exposure limit (PEL; 8 hr) set by the U.S. Occupational Safety and Health Administration. Objective: We investigated whether occupational exposures to benzene near 1 ppm induce aneuploidy in sperm. Methods: We used multicolor fluorescence in situ hybridization to measure the incidence of sperm with numerical abnormalities of chromosomes X, Y, and 21 among 33 benzene-exposed men and 33 unexposed men from Chinese factories. Individual exposures were assessed using personal air monitoring and urinary concentrations of benzene and trans,trans-muconic acid (E,E-MA). Air benzene concentrations were not detectable in unexposed men; in exposed men, concentrations ranged from below the detection limit to 24 ppm (median, 2.9 ppm), with 27% of exposed men (n = 9) having concentrations of ≥ 1 ppm. Exposed men were categorized into low and high groups based on urinary E,E-MA (median concentrations of 1.9 and 14.4 mg/L, respectively; median air benzene of 1 and 7.7 ppm, respectively), and aneuploidy frequencies were compared with those of unexposed men. Results: Sperm aneuploidy increased across low- and high-exposed groups for disomy X [incidence rate ratio (IRR) = 2.0; 95% confidence interval (CI), 1.1-3.4; and IRR = 2.8; 95% CI, 1.5-4.9, respectively], and for overall hyperhaploidy for the three chromosomes investigated (IRR = 1.6; 95% CI, 1.0-2.4; and IRR = 2.3; 95% CI, 1.5-3.6, respectively). We also found elevated disomy X and hyperhaploidy in the nine men exposed to ≥ 1 ppm benzene compared with unexposed men (IRR = 1.8; 95% CI, 1.1-3.0; and IRR = 2.0; 95% CI, 1.1-3.9, respectively). Conclusions: Benzene appeared to increase the frequencies of aneuploid sperm for chromosomes associated with chromosomal abnormality syndromes in human offspring, even in men whose air benzene exposure was at or below the U.S. permissible exposure limit. [ABSTRACT FROM AUTHOR]
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- 2010
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33. Evidence That Humans Metabolize Benzene via Two Pathways.
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Rappaport, Stephen M., Sungkyoon Kim, Qing Lan, Vermeulen, Roel, Waidyanatha, Suramya, Luoping Zhang, Guilan Li, Songnian Yin, Hayes, Richard B., Rothman, Nathaniel, and Smith, Martyn T.
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BENZENE , *AFFERENT pathways , *METABOLITES , *ENZYMES , *POPULATION , *BIOCHEMISTRY - Abstract
BACKGROUND: Recent evidence has shown that humans metabolize benzene more efficiently at environmental air concentrations than at concentrations > 1 ppm. This led us to speculate that an unidentified metabolic pathway was mainly responsible for benzene metabolism at ambient levels. OBJECTIVE: We statistically tested whether human metabolism of benzene is better fitted by a kinetic model having two pathways rather than one. METHODS: We fit Michaelis-Menten-like models to levels of urinary benzene metabolites and the corresponding air concentrations for 263 nonsmoking Chinese females. Estimated benzene concentrations ranged from less than 0.001 ppm to 299 ppm, with 10th and 90th percentile values of 0.002 ppm and 8.97 ppm, respectively. RESULTS: Using values of Akaike's information criterion obtained under the two models, we found strong statistical evidence favoring two metabolic pathways, with respective affinities (benzene air concentrations analogous to Km values) of 301 ppm for the low-affinity pathway (probably dominated by cytochrome P450 enzyme 2E1) and 0.594 ppm for the high-affinity pathway (unknown). The exposure-specific metabolite level predicted by our two-pathway model at nonsaturating concentrations was 184 μM/ppm of benzene, a value close to an independent estimate of 194 μM/ppm for a typical nonsmoking Chinese female. Our results indicate that a nonsmoking woman would metabolize about three times more benzene from the ambient environment under the two-pathway model (184 μM/ppm) than under the one-pathway model (68.6 μM/ppm). In fact, 73% of the ambient benzene dose would be metabolized via the unidentified high-affinity pathway. CONCLUSION: Because regulatory risk assessments have assumed nonsaturating metabolism of benzene in persons exposed to air concentrations well above 10 ppm, our findings suggest that the true leukemia risks could be substantially greater than currently thought at ambient levels of exposure- about 3-fold higher among nonsmoking females in the general population. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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34. Linking the concept of ecological footprint and valuation of ecosystem services - a case study of economic growth and natural carrying capacity.
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Sheng Zhao, Changwen Wu, Huasheng Hong, and Luoping Zhang
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BIOTIC communities , *SUSTAINABLE development , *RENEWABLE energy sources , *ECOLOGICAL economics , *ENVIRONMENTAL sciences - Abstract
Human activities have become so extensive that all ecosystems on the planet have been altered to some extent. The fate of humankind will be determined by how sustainable ecosystems and renewable resource species in them are managed. The implication of this is obvious: humanity must live within nature's carrying capacity. In recent years, we have recognized that economic growth depends on natural capital, the importance of identifying ourselves as a part of the international ecological economics community, and positive integration of economy and ecology. The aim of this paper is to describe a method for integrated analysis of economic growth and natural carrying capacity through linking the concept of ecological footprint with valuation of ecosystem services. When applied to China for the period 1987-2003, empirical evidence suggests that the Chinese economy surpassed its carrying capacity after 1992. Perhaps we should abandon our high-growth predilection and initiate the transition to a steady-state economy. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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35. Genotype frequency and F ST analysis of polymorphisms in immunoregulatory genes in Chinese and Caucasian populations.
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Qing Lan, Min Shen, Garcia-Rossi, Dino, Chanock, Stephen, Tongzhang Zheng, Berndt, Sonja, Puri, Vinita, Guilan Li, Xingzhou He, Welch, Robert, Zahm, Shelia, Luoping Zhang, Yawei Zhang, Smith, Martyn, Wang, Sophia, Chiu, Brian, Linet, Martha, Hayes, Richard, Rothman, Nathaniel, and Yeager, Meredith
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GENETIC research , *CYTOKINES , *CHEMOKINES , *PEPTIDES , *PROTEINS - Abstract
Selection and genetic drift can create genetic differences between populations. Cytokines and chemokines play an important role in both hematopoietic development and the inflammatory response. We compared the genotype frequencies of 45 SNPs in 30 cytokine and chemokine genes in two healthy Chinese populations and one Caucasian population. Several SNPs in IL4 had substantial genetic differentiation between the Chinese and Caucasian populations ( F ST ~0.40), and displayed a strikingly different haplotype distribution. To further characterize common genetic variation in worldwide populations at the IL4 locus, we genotyped 9 SNPs at the IL4 gene in the Human Diversity Panel’s ( N = 1056) individuals from 52 world geographic regions. We observed low haplotype diversity, yet strikingly different haplotype frequencies between non-African populations, which may indicate different selective pressures on the IL4 gene in different parts of the world. SNPs in CSF2, IL6, IL10, CTLA4, and CX3CR1 showed moderate genetic differentiation between the Chinese and Caucasian populations (0.15 < F ST < 0.25). These results suggest that there is substantial genetic diversity in immune genes and exploration of SNP associations with immune-related diseases that vary in incidence across these two populations may be warranted. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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36. Albumin Adducts of Electrophilic Benzene Metabolites in Benzene-Exposed and Control Workers.
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Yu-Sheng Lin, Vermeulen, Roel, Tsai, Chin H., Waidyanatha, Suramya, Qing Lan, Rothman, Nathaniel, Smith, Martyn T., Luoping Zhang, Min Shen, Guilan Li, Songnian Yin, Kim, Sungkyoon, and Rappaport, Stephen M.
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BENZENE in the body , *BENZENE biodegradation , *BIOCHEMISTRY , *BLOOD testing , *ALBUMINS , *METABOLISM , *MUTAGENS , *DNA adducts - Abstract
BACKGROUND: Metabolism of benzene produces reactive electrophiles, including benzene oxide (BO), 1,4-benzoquinone (1,4-BQ), and 1,2-benzoquinone (1,2-BQ), that are capable of reacting with blood proteins to produce adducts. OBJECTIVES: The main purpose of this study was to characterize relationships between levels of albumin adducts of these electrophiles in blood and the corresponding benzene exposures in benzene-exposed and control workers, after adjusting for important covariates. Because second blood samples were obtained from a subset of exposed workers, we also desired to estimate within-person and between-person variance components for the three adducts. METHODS: We measured albumin adducts and benzene exposures in 250 benzene-exposed workers (exposure range, 0.26-54.5 ppm) and 140 control workers (exposure range < 0.01-0.53 ppm) from Tianjin, China. Separate multiple linear regression models were fitted to the logged adduct levels for workers exposed to benzene < 1 ppm and ≥ 1 ppm. Mixed-effects models were used to estimate within-person and between-person variance components of adduct levels. RESULTS: We observed nonlinear (hockey-stick shaped) exposure-adduct relationships in log-scale, with inflection points between about 0.5 and 5 ppm. These inflection points represent air concentrations at which benzene contributed marginally to background adducts derived from smoking and from dietary and endogenous sources. Adduct levels were significantly affected by the blood-collection medium (serum or plasma containing either heparin or EDTA), smoking, age, and body mass index. When model predictions of adduct levels were plotted versus benzene exposure ≥ 1 ppm, we observed marked downward concavity, particularly for adducts of the benzoquinones. The betweenperson variance component of adduct levels increased in the order 1,2-BQ < 1,4-BQ < BO, whereas the within-person variance components of the three adducts followed the reverse order. CONCLUSIONS: Although albumin adducts of BO and the benzoquinones reflect exposures to benzene ≥ 1 ppm, they would not be useful biomarkers of exposure at ambient levels of benzene, which tend to be < 0.01 ppm, or in those working populations where exposures are consistently < 1 ppm. The concavity of exposure-adduct relationships is consistent with saturable metabolism of benzene at air concentrations > 1 ppm. The surprisingly large effect of the blood-collection medium on adduct levels, particularly those of the benzoquinones, should be further investigated. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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37. The Flavoring Agent Dihydrocoumarin Reverses Epigenetic Silencing and Inhibits Sirtuin Deacetylases.
- Author
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Olaharski, Andrew J., Rine, Jasper, Marshall, Brett L., Babiarz, Joshua, Luoping Zhang, Verdin, Eric, and Smith, Martyn T.
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NICOTINAMIDE , *NAD (Coenzyme) , *AGING , *SACCHAROMYCES , *YEAST , *AMIDES , *ADENINE - Abstract
Sirtuins are a family of phylogenetically conserved nicotinamide adenine dinucleotide-dependent deacetylases that have a firmly established role in aging. Using a simple Saccharomyces cerevisiae yeast heterochromatic derepression assay, we tested a number of environmental chemicals to address the possibility that humans are exposed to sirtuin inhibitors. Here we show that dihydrocoumarin (DHC), a compound found in Melilotus officinalis (sweet clover) that is commonly added to food and cosmetics, disrupted heterochromatic silencing and inhibited yeast Sir2p as well as human SIRT1 deacetylase activity. DHC exposure in the human TK6 lymphoblastoid cell line also caused concentration-dependent increases in p53 acetylation and cytotoxicity. Flow cytometric analysis to detect annexin V binding to phosphatidylserine demonstrated that DHC increased apoptosis more than 3-fold over controls. Thus, DHC inhibits both yeast Sir2p and human SIRT1 deacetylases and increases p53 acetylation and apoptosis, a phenotype associated with senescence and aging. These findings demonstrate that humans are potentially exposed to epigenetic toxicants that inhibit sirtuin deacetylases. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
38. Persistent Organochlorine Pollutants in Surface Sediments and Economic Shellfish of Minjiang Estuary-Mazu and Xiamen-Jinmen Sea Areas.
- Author
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Weiqi Chen, Huasheng Hong, Li Xu, Luoping Zhang, Xinhong Wang, and Liyu Hong
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ORGANOCHLORINE compounds , *POLLUTANTS , *SHELLFISH , *ESTUARIES , *SEDIMENTS - Abstract
Samples of surface sediment and shellfish were collected from Minjiang Estuary-Mazu and Xiamen-Jinmen sea areas and were analyzed for hexachlorocyclohexanes (HCHs), dichlorodiphenyl-trichlorophenyl (DDT) and its degraded derivatives dichloro-diphenyl-dichloroethane (DDT) and dichloro-dichlorophenyl-ethylene (DDE), and polychlorinated biphenyls (PCBs) by gas chromatography. Concentration ranges of HCHs, DDTs, and PCBs in the sediments were 0.03–0.48, 1.10–14.3, and ND to 0.76 ng/g dw, respectively; those for shellfish samples were ND to 1.5, 21.5–892, dw and ND–3.7 ng/g dw, respectively. The results showed that the concentrations of such oganochlorine compounds (OCs) in the sediments were usually low as compared with other sea areas and estuaries, and the organochlorines were accumulated in marine bivalve mollusks, particularly in oyster. With respect to the concentrations of HCHs, DDTs, and PCBs in the samples of either sediment or shellfish, DDTs were higher than HCHs and PCBs. The concentrations of OCs in shellfish samples varied with the sampling locations and organism species but were generally higher than those in the sediments. The concentration distributions, compositions, and residue levels of these OCs are extensively discussed and evaluated. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
39. Discovery of Novel Biomarkers by Microarray Analysis of Peripheral Blood Mononuclear Cell Gene Expression in Benzene-Exposed Workers.
- Author
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Forrest, Matthew S., Qing Lan, Hubbard, Alan E., Luoping Zhang, Vermeulen, Roel, Xin Zhao, Guilan Li, Yen-Ying Wu, Min Shen, Songnian Yin, Chanock, Stephen J., Rothman, Nathaniel, and Smith, Martyn T.
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BENZENE , *AROMATIC compounds , *LEUKEMIA , *GENE expression , *GENETIC regulation , *ENVIRONMENTAL health - Abstract
Benzene is an industrial chemical and component of gasoline that is an established cause of leukemia. To better understand the risk benzene poses, we examined the effect of benzene exposure on peripheral blood mononuclear cell (PBMC) gene expression in a population of shoe factory workers with well-characterized occupational exposures using microarrays and real-time polymerase chain reaction (PCR). PBMC RNA was stabilized in the field and analyzed using a comprehensive human array, the U133A/B Affymetrix GeneChip set. A matched analysis of six exposed-control pairs was performed. A combination of robust multiarray analysis and ordering of genes using paired t-statistics, along with bootstrapping to control for a 5% familywise error rate, was used to identify differentially expressed genes in a global analysis. This resulted in a set of 29 known genes being identified that were highly likely to be differentially expressed. We also repeated these analyses on a smaller subset of 508 cytokine probe sets and found that the expression of 19 known cytokine genes was significantly different between the exposed and the control subjects. Six genes were selected for confirmation by real-time PCR, and of these, CXCL16, ZNF331, JUN, and PF4 were the most significantly affected by benzene exposure, a finding that was confirmed in a larger data set from 28 subjects. The altered expression was not caused by changes in the makeup of the PBMC fraction. Thus, microarray analysis along with real-time PCR confirmation reveals that altered expressions of CXCL16, ZNF331, JUN, and PF4 are potential biomarkers of benzene exposure. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
40. Genetic variants at 6p21.33 are associated with susceptibility to follicular lymphoma.
- Author
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Skibola, Christine F., Bracci, Paige M., Halperin, Eran, Conde, Lucia, Craig, David W., Agana, Luz, Iyadurai, Kelly, Becker, Nikolaus, Brooks-Wilson, Angela, Curry, John D., Spinelli, John J., Holly, Elizabeth A., Riby, Jacques, Luoping Zhang, Nieters, Alexandra, Smith, Martyn T., and Brown, Kevin M.
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DISEASE susceptibility , *LYMPHOMAS , *LEUKEMIA , *PSORIASIS , *CLINICAL trials - Abstract
We conducted genome-wide association studies of non-Hodgkin lymphoma using Illumina HumanHap550 BeadChips to identify subtype-specific associations in follicular, diffuse large B-cell and chronic lymphocytic leukemia/small lymphocytic lymphomas. We found that rs6457327 on 6p21.33 was associated with susceptibility to follicular lymphoma (FL; N = 189 cases, 592 controls) with validation in another 456 FL cases and 2,785 controls (combined allelic P = 4.7 × 10−11). The region of strongest association overlapped C6orf15 (STG), located near psoriasis susceptibility region 1 (PSORS1). [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
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41. Werner Syndrome Protein, WRN, Protects Cells from DNA Damage Induced by the Benzene Metabolite Hydroquinone.
- Author
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Xuefeng Ren, Sophia Lim, Martyn T. Smith, and Luoping Zhang
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DNA helicases , *WERNER'S syndrome , *DNA damage , *GENETIC mutation , *HYDROQUINONE , *ANTIBODY-dependent cell cytotoxicity , *CELL growth , *GENOMICS , *PREVENTION - Abstract
Werner syndrome (WS) is a rare autosomal progeroid disorder caused by a mutation in the gene encoding the WRN (Werner syndrome protein), a member of the RecQ family of helicases with a role in maintaining genomic stability. Genetic association studies have previously suggested a link between WRN and susceptibility to benzene-induced hematotoxicity. To further explore the role of WRN in benzene-induced hematotoxicity, we used short hairpin RNA to silence endogenous levels of WRN in the human HL60 acute promyelocytic cell line and subsequently exposed the cells to hydroquinone (HQ). Suppression of WRN led to an accelerated cell growth rate, increased susceptibility to hydroquinone-induced cytotoxicity and genotoxicity as measured by the single-cell gel electrophoresis assay, and an enhanced DNA damage response. More specifically, loss of WRN resulted in higher levels of early apoptosis, marked by increases in relative levels of cleaved caspase-7 and cleaved poly (ADP-ribose) polymerase 1, in cells treated with HQ compared with control cells. Our data suggests that WRN plays an important role in the surveillance of and protection against DNA damage induced by HQ. This provides mechanistic support for the link between WRN and benzene-induced hematotoxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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42. Decreased Urinary Beta-Defensin-1 Expression as a Biomarker of Response to Arsenic.
- Author
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Christine M. Hegedus, Christine F. Skibola, Marcella Warner, Danica R. Skibola, David Alexander, Sophia Lim, Nygerma L. Dangleben, Luoping Zhang, Michael Clark, Ruth M. Pfeiffer, Craig Steinmaus, Allan H. Smith, Martyn T. Smith, and Lee E. Moore
- Subjects
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ARSENIC , *CONTAMINATION of drinking water , *TOXICITY testing , *BIOMARKERS - Abstract
Ingestion of arsenic (As) through contaminated drinking water results in increased risks of skin, lung, kidney, and bladder cancers. Due to its association with kidney and bladder cancers, we hypothesized that analysis of the urinary proteome could provide insight into the mechanisms of As toxicity. Urine from participants in a cross-sectional As biomarker study conducted in Nevada, classified as having either high (⥠100 μg total urinary As/l) or low exposure (p in vitro experiment, gene expression analysis of As-treated cell lines demonstrated reduced HBD1 mRNA confirming that the observed decrease in HBD-1 resulted from As exposure. HBD-1 is an antimicrobial peptide constitutively expressed in multiple tissues including epithelial cells of the respiratory and urogenital systems. Recent studies support its role as a tumor suppressor gene for urological cancers suggesting that decreased HBD-1 levels may play a role in the development of cancers associated with As exposure. Further studies are warranted to investigate the role of HBD-1 in As-related toxicity. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
43. Leukaemia-specific chromosome damage detected by comet with fluorescence in situ hybridization (comet-FISH).
- Author
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Patricia A. Escobar, Martyn T. Smith, Ananth Vasishta, Alan E. Hubbard, and Luoping Zhang
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GENES , *NUCLEIC acids , *AROMATIC compounds , *GENETICS - Abstract
Acute myeloid leukaemia (AML) is associated with exposure to benzene and treatment with chemotherapeutic agents. It is thought to arise from damage to specific regions of DNA, resulting in chromosome rearrangements or loss. For instance, a deletion on the long arm of chromosome 5 [e.g. del(5q31)] is common in AML patients previously treated with alkylating agents, such as melphalan, or exposed to benzene. Translocations of the MLL gene at 11q23 are frequently observed in AML arising from treatment with topoisomerase II inhibitors, such as etoposide. Our goal was to determine whether or not breakage at 5q31 and 11q23 is selectively induced by these chemical agents. To address this question, the comet assay combined with fluorescence in situ hybridization (cometâFISH) was used to detect DNA breakage in the specific chromosomal regions in an in vitro model. TK6 lymphoblastoid cells were exposed to melphalan, etoposide or the benzene metabolite, hydroquinone (HQ), at various concentrations. HQ, melphalan and etoposide induced DNA breaks at both 5q31 and 11q23 chromosome regions in a dose-dependant manner. However, HQ produced significantly more DNA damage at 5q31 than at 11q23. Etoposide produce slightly more DNA damage at 11q23 and melphalan had a somewhat greater effect at 5q31, but not significantly so. Thus, HQ and melphalan act similarly, perhaps explaining some similarities between benzene- and alkylating agent-induced AML. CometâFISH also appears to be a useful approach for detecting and comparing damage to specific chromosome regions of significance in leukaemogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
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