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Global Gene Expression Profiling of a Population Exposed to a Range of Benzene Levels.
- Source :
-
Environmental Health Perspectives . May2011, Vol. 119 Issue 5, p628-634. 7p. 2 Diagrams, 4 Charts, 1 Graph. - Publication Year :
- 2011
-
Abstract
- Background: Benzene, an established cause of acute myeloid leukemia (AML), may also cause one or more lymphoid malignancies in humans. Previously, we identified genes and pathways associated with exposure to high (> 10 ppm) levels of benzene through transcriptomic analyses of blood cells from a small number of occupationally exposed workers. Objectives: The goals of this study were to identify potential biomarkers of benzene exposure and/or early effects and to elucidate mechanisms relevant to risk of hematotoxicity, leukemia, and lymphoid malignancy in occupationally exposed individuals, many of whom were exposed to benzene levels < 1 ppm, the current U.S. occupational standard. Methods: We analyzed global gene expression in the peripheral blood mononuclear cells of 125 workers exposed to benzene levels ranging from < 1 ppm to > 10 ppm. Study design and analysis with a mixed-effects model minimized potential confounding and experimental variability. Results: We observed highly significant widespread perturbation of gene expression at all exposure levels. The AML pathway was among the pathways most significantly associated with benzene exposure. Immune response pathways were associated with most exposure levels, potentially providing biological plausibility for an association between lymphoma and benzene exposure. We identified a 16-gene expression signature associated with all levels of benzene exposure. Conclusions: Our findings suggest that chronic benzene exposure, even at levels below the current U.S. occupational standard, perturbs many genes, biological processes, and pathways. These findings expand our understanding of the mechanisms by which benzene may induce hematotoxicity, leukemia, and lymphoma and reveal relevant potential biomarkers associated with a range of exposures. [ABSTRACT FROM AUTHOR]
- Subjects :
- *BENZENE
*BIOMARKERS
*COMPUTER software
*DOSE-effect relationship in pharmacology
*GENE expression
*GENETIC techniques
*INDOOR air pollution
*MOLECULAR epidemiology
*PATH analysis (Statistics)
*PROBABILITY theory
*RESEARCH funding
*STATISTICS
*OCCUPATIONAL hazards
*DATA analysis
*ENVIRONMENTAL exposure
*INTER-observer reliability
*CROSS-sectional method
*MICROARRAY technology
Subjects
Details
- Language :
- English
- ISSN :
- 00916765
- Volume :
- 119
- Issue :
- 5
- Database :
- Academic Search Index
- Journal :
- Environmental Health Perspectives
- Publication Type :
- Academic Journal
- Accession number :
- 60677465
- Full Text :
- https://doi.org/10.1289/ehp.1002546