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Background: Sex-determining region Y-box 2 (SOX2) is a transcriptional factor that drives embryonic stem cells to neuroendocrine cells in lung development and is highly expressed in small-cell lung cancer (SCLC). However, the prognostic role of SOX2 and its relationship with tumor-infiltrating lymphocytes (TILs) has not been determined in SCLC. Herein, we assessed the expression of SOX2 and CD8+ TILs to obtain insights into the prognostic role of SOX2 and CD8+ TILs in limited-stage (LS)-SCLC. Methods: A total of 75 patients with LS-SCLC was enrolled. The SOX2 expression and CD8+ TILs were evaluated by immunohistochemistry. Results: High SOX2 and CD8+ TIL levels were identified in 52 (69.3%) and 40 (53.3%) patients, respectively. High SOX2 expression was correlated with increased density of CD8+ TILs (p = 0.041). Unlike SOX2, high CD8+ TIL numbers were associated with significantly longer progression-free survival (PFS; 13.9 vs. 8.0 months, p = 0.014). Patients with both high SOX2 expression and CD8+ TIL numbers (n = 29, 38.7%) had significantly longer PFS and overall survival (OS) compared to those from the other groups (median PFS 19.3 vs. 8.4 months; p = 0.002 and median OS 35.7 vs. 17.4 months; p = 0.004, respectively). Multivariate Cox regression analysis showed that the combination of high SOX2 expression and CD8+ TIL levels was an independent good prognostic factor for OS (HR = 0.471, 95% CI, 0.250–0.887, p = 0.02) and PFS (HR = 0.447, 95% CI, 0.250–0.801, p = 0.007) in SCLC. Conclusions: Evaluation of the combination of SOX2 and CD8+ TIL levels may be of a prognostic value in LS-SCLC. [ABSTRACT FROM AUTHOR]

This article discusses the growing recognition of the importance of environmental, social, and corporate governance (ESG) principles in various industries, particularly in relation to the United Nations' Sustainable Development Goals (SDGs). The article highlights the lack of comprehensive research on ESG as a strategic tool in the academic domain. The special issue of the journal focuses on ESG management and marketing concerns in the tourism and hospitality industry, featuring 10 empirical papers from researchers in different countries. The articles cover topics such as corporate governance, ESG practices in the restaurant industry, consumer skepticism, employee behavior, web accessibility, and the involvement of international tourism organizations in incorporating SDGs. The authors express their appreciation to the contributors and reviewers of the special issue. [Extracted from the article]

Cerium oxide nanoparticles (CeO2 NPs) are widely used in various commercial applications because of their characteristic properties. People can be easily exposed to CeO2 NPs in real life, but the safety assessment of CeO2 NPs has not been fully investigated. Therefore, in this study, we conducted a combined repeated-dose and reproductive/developmental toxicity screening study (OECD testing guideline 422) to investigate the potential hazards on human health, including reproductive/developmental functions, after repeated daily CeO2 NPs oral gavage administration to both males and females. In addition, tissues from parental animals and their pups were collected to analyze the internal accumulation of cerium. CeO2 NPs were orally administered to Sprague-Dawley rats at doses of 0, 100, 300 and 1000 mg/kg during their pre-mating, mating, gestation and early lactation periods. In the general systemic and reproductive/developmental examinations, no marked toxicities were observed in any in-life and terminal observation parameters in this study. In the biodistribution analysis, cerium was not detected in either parental or pup tissues (blood, liver, lungs and kidneys). Repeated oral exposure of CeO2 NPs did not induce marked toxicities affecting general systemic and reproductive/developmental functions up to the dose level of 1000 mg/kg and the CeO2 NPs were not systemically absorbed in parental animals or their pups. This result could be used in risk assessment for humans, and additional toxicity studies with CeO2 NPs will be necessary considering various physicochemical properties and exposure probabilities of these nanoparticles. [ABSTRACT FROM AUTHOR]

Deer (Cervus elaphus Linnaeus) antler extract has been traditionally used in many Asian countries to prevent and treat various diseases. Deer antler extract is generally considered to be safe because humans have been exposed to it for a long time and it has been used as a tonic medicine originating from naturally occurring product. However, the comprehensive toxicological potential of deer antler extract during the juvenile period has not been investigated and its safety for juveniles remains unclear. The aim of the present study was to comprehensively investigate the potential toxicological effects of deer antler extract during the juvenile period. As part of a safety assessment of the juvenile period, two separate studies, a juvenile toxicity study and a uterotrophic bioassay, were conducted in accordance with Good Laboratory Practice regulations and test guidelines. In the juvenile toxicity study, deer antler extract was administered daily by oral gavage to Sprague-Dawley rats at doses of 0, 500, 1000, and 2000 mg/kg during the juvenile period to investigate potential toxicities on general systemic, developmental, and reproductive functions. For the uterotrophic bioassay, deer antler extract was administered daily by oral gavage to Sprague-Dawley rats at doses of 0, 1000, and 2000 mg/kg on postnatal days 19–21 to investigate the estrogen-mimicking effects. The results showed that deer antler extract was non-toxic and no observable adverse effects on general systemic function, developmental and reproductive function, and estrogen-mimetic effects were observed with dosing up to 2000 mg/kg during the juvenile period. The safety of deer antler extracts was demonstrated in these studies and the results of this study can be used to evaluate human risk or determine the maximum recommended starting dose of deer antler extract for further clinical trials. [Display omitted] • We investigated the effects of deer antler extract during the juvenile period. • A juvenile toxicity study and a uterotrophic bioassay were conducted with rats. • Deer antler extract was considered non-toxic up to 2000 mg/kg/day. [ABSTRACT FROM AUTHOR]

Objective. Herbal medicine has been widely used to treat drug resistant epilepsy. Shihogyejitang (SGT) has been commonly used to treat epilepsy. We investigated the effect and safety of SGT in children with drug resistant epilepsy. Design. We reviewed medical records of 54 patients with epilepsy, who failed to respond to at least two antiepileptic drugs and have been treated with SGT between April 2006 and June 2014 at the Department of Pediatric Neurology, I-Tomato Hospital, Korea. Effect was measured by the response rate, seizure-free rate, and retention rate at six months. We also checked adverse events, change in antiepileptic drugs use, and the variables related to the outcome. Results. Intent-to-treat analysis showed that, after six months, 44.4% showed a >50% seizure reduction, 24.1% including seizure-free, respectively, and 53.7% remained on SGT. Two adverse events were reported, mild skin rash and fever. Focal seizure type presented significantly more positive responses when compared with other seizure types at six months (p=0.0284, Fisher’s exact test). Conclusion. SGT is an effective treatment with excellent tolerability for drug resistant epilepsy patients. Our data provide evidence that SGT may be used as alternative treatment option when antiepileptic drug does not work in epilepsy children. [ABSTRACT FROM AUTHOR]

Methiozolin is a new herbicide to control annual bluegrass ( Poa annua L.) and large crabgrass ( Digitaria sanguinalis (L.) Scop.) in various turfgrasses. The potential of methiozolin to induce maternal and developmental toxicity was investigated in the pregnant New Zealand White Rabbits. Methiozolin was, at dose levels of 0, 125, 250 and 500 mg/kg/day, administered by oral gavage to artificially inseminated rabbits (25 females per group) from days 6 to 28 of gestation. All does were subjected to Cesarean section on day 29 of gestation. At 500 mg/kg/day, treatment-related toxicities including abortion (10/22), decreased mean body weight, weight gain, net body weight change, reduced food consumption and decreased fetal weight were observed. At 125 and 250 mg/kg/day, no signs of maternal and developmental toxicity were observed. There were no treatment-related external, visceral and skeletal abnormalities of fetuses at all doses tested. In the current experimental conditions, the no observed adverse effect levels (NOAELs) of methiozolin are considered to be 250 mg/kg/day for does and prenatal development. [ABSTRACT FROM AUTHOR]

This research builds on the idea that casino customers’ subjective, global judgments of service quality are shaped by experience of its specific attributes, and examines how casino customers form their evaluative attitudes toward the casino experience in the Macau context. Using the data collected from 525 Macau casino customers, we first identify four casino quality dimensions; namely, supporting facilities, staff service, diversity, and equitableness. We then test and validate a proposed model for the structural relationships between casino image (as a consequence of the four quality dimensions), value, and loyalty. In particular, value is shown to be a mediator of the relationship between image and loyalty from the perspective of casino customers. The results of our study can show Macau casino operators what constitutes customers’ casino experiences and aid them in enhancing their image, perceived value, and customer loyalty toward casinos. [ABSTRACT FROM AUTHOR]

Abstract: Here we demonstrate that indium tin oxide (ITO) films deposited on glass can be directly patterned by a spatially -modulated pulsed Nd-YAG laser beam (wavelength=1064nm, pulse width=6ns) incident onto the film. This method utilizes a pulsed laser-induced thermo-elastic force exerting on the film which plays a role to detach it from the substrate. Sharp-edged clean patterns with feature size as small as 4μm could be obtained. The threshold pulse energy density for patterning was estimated to be ∼0.8J/cm2 for 150nm-thick ITO film, making it possible to pattern over one square centimeter by a single pulse with energy of 850mJ. Not only being free from photoresist and chemical etching steps, the presented method can also provide much higher throughput than the tradition photoablation process utilizing a tightly focused beam. [Copyright &y& Elsevier]

Inhalation exposure to polyhexamethylene guanidine phosphate (PHMG-P), one of the primary biocides used in humidifier disinfectants, caused a fatal pulmonary disease in Korea. Pregnant women were also exposed to PHMG-P, and subsequent studies showed that PHMG-P inhalation during pregnancy adversely affects their health and embryo-fetal development. However, the postnatal developmental effects after birth on prenatally PHMG-P-exposed offspring have not yet been investigated. Therefore, in this study, we aimed to examine the postnatal development of prenatally PHMG-P-exposed offspring. Pregnant rats (22 or 24 females per group) were exposed to PHMG-P during pregnancy in a whole-body inhalation chamber at the target concentrations of 0, 0.14, 1.60, and 3.20 mg/m3. After parturition, the prenatally exposed offspring were transferred to non-exposed surrogate mothers to minimize the secondary effects of severe maternal toxicities. Postnatal development of offspring was then examined with a modified extended one-generation reproductive toxicity study design. At 3.20 mg/m3 PHMG-P, increased perinatal death rates and decreased viability index (postnatal survival of offspring between birth and postnatal day 4) were observed. In addition, F1 offspring had lower body weight at birth that persisted throughout the study. PHMG-P-exposed pregnant rats also had severe systemic toxicities and increased gestation period. At 1.60 mg/m3 PHMG-P, a decreased viability index was also observed with systemic toxicities of PHMG-P-exposed pregnant rats. These results indicate that prenatal PHMG-P exposure adversely affects the offspring's future health and could be used for human risk assessment. • Postnatal development of prenatally PHMG-P-exposed offspring was investigated. • PHMG-P increased perinatal death and decreased postnatal survival after birth. • PHMG-P decreased the body weight of offspring after birth. • PHMG-P increased the gestation period of PHMG-P inhaled pregnant rats. • Adverse effects in offspring were observed with maternal toxicities during pregnancy. [ABSTRACT FROM AUTHOR]

Intratracheal instillation (ITI) is considered the most pragmatic approach for investigating the potential toxicities of various respiratory exposure materials. Various respiratory exposure materials, including nanomaterials, hazardous air pollutants, fine dust, and household biocides, have raised public health concerns because of limited toxicological information and increasing consumption. Hence, toxicity studies using ITI in laboratory animals are important to accurately assess the human risks associated with these respiratory-exposed materials. However, data to adequately support the study design of ITI toxicity studies, particularly those examining the effects of commonly used vehicles following repeated exposure are insufficient. Therefore, in this study, we examined the effects of 16 types of commonly used vehicles in toxicity studies following 14-day repeated ITI in mice. General health endpoints (mortality, clinical signs, and body weight) were monitored throughout the study period, and terminal endpoints (gross observation, lung weight, bronchoalveolar lavage fluid analysis, and lung histopathological examination) were assessed after terminal sacrifice. Saline and phosphate-buffered saline elicited the least response, whereas corn oil (50 µL) showed the most severe toxicity findings. In addition, several commonly used vehicles, including distilled water, sodium carboxymethyl cellulose, dimethyl sulfoxide, ethanol, Tween 20, and Tween 80, induced mild-to-severe toxicity in the respiratory system. Based on the results of this study, some commonly used vehicles in toxicity studies should be used with caution when the ITI exposure route is considered. These results provide important background information on the effects of vehicles in ITI toxicity studies along with valuable insights for designing toxicity studies using respiratory exposure materials. [ABSTRACT FROM AUTHOR]

Serial blood sampling for toxicokinetics is generally conducted in regulatory embryo-fetal development (EFD) studies in rats. EFD studies are designed to detect the potential adverse effects of pharmaceuticals on pregnant females and their fetuses; this information is useful for understanding the relationships between systemic exposure levels and toxicity profiles. However, additional satellite pregnant females are needed for toxicokinetics because comprehensive information regarding the potential impact of serial blood sampling on pregnant females is scarce. Here, in this study, we investigated the potential impact of serial blood sampling in pregnant female rats using a typical EFD study design. Additionally, we investigated the additional endpoints (clinical pathology, organ weights, and histopathology) that were deemed likely to be sensitive to blood sampling. Results indicated that serial blood sampling in pregnant females induced physiological adaptive changes and did not affect the general endpoints in EFD studies. Nevertheless, inclusion of satellite groups in EFD studies may be a more prudent approach considering the physiological changes in pregnant females and potential off-target effects of candidate pharmaceuticals. These results provide background information on the impact of serial blood sampling in pregnant females and will be useful to design the regulatory EFD studies. • We investigated the effects of serial blood sampling in pregnant female rats. • Serial blood sampling in pregnant females induced physiological adaptive changes. • Inclusion of satellite groups in EFD studies may be a more prudent approach. [ABSTRACT FROM AUTHOR]

Biocides are widely used for their effective antiseptic and disinfectant properties, including polyhexamethylene guanidine phosphate (PHMG-P), which is also used as a biocide as it selectively disrupts bacterial cell membrane. It is used to clean humidifiers commonly used in the dry winter season in South Korea, which exposes people to PHMG-P inhalation. However, comprehensive toxicological data on PHMG-P inhalation exposure, including in pregnant women, and the potential occurrence of lung disease is lacking. Therefore, in this study, we investigated PHMG-P inhalation exposure-induced toxicities in pregnant rats and prenatal development of their conceptus. Pregnant rats were exposed to PHMG-P via inhalation at target concentrations of 0, 0.14, 1.60, and 3.20 mg/m3 from implantation to nearly parturition (from gestation day 6–20) and then analyzed for relevant abnormalities. Results showed systemic toxicities in the pregnant rats including respiratory function abnormalities, decreased body weight gain, and decreased food consumption at ≥1.60 mg/m3. Prenatal development toxicities, including decreased fetal weight with ossification retardations of fetal bones, were observed at 3.20 mg/m3. These results will contribute to clarifying the PHMG-P inhalation exposure-induced toxicities during pregnancy and support its risk assessment in humans. ga1 • PHMG-P inhalation exposure during pregnancy induced toxicities in pregnant rats and their conceptus. • PHMG-P inhalation exposure induced respiratory function abnormalities of pregnant rats at ≥1.60 mg/m3 PHMG-P. • PHMG-P inhalation exposure induced intrauterine growth retardation of fetuses at 3.20 mg/m3 PHMG-P. [ABSTRACT FROM AUTHOR]

We show that the photocurrent and energy conversion efficiency of dye-sensitized solar cells can be greatly enhanced with holographic modification to the morphology of TiO2 electrode. The nanoporous electrode coated onto conducting glass was irradiated by three interfering laser beams at 1064 nm incident from the backside of the substrate. This generated two-dimensional periodic pillars of higher density in the electrode, through which the photoexcited electrons could be extracted more effectively. The cells fabricated with modified electrodes exhibited average photocurrent and efficiency of 17.14 mA/cm2 and 9.03%, while 14.91 mA/cm2 and 7.83% were obtained from the reference cells. It was attributed to the enhanced charge transport accompanied by the reduction of internal resistance of the electrode. [ABSTRACT FROM AUTHOR]

2,5-Pyridinedicarboxylic acid derivatives were found to be the potent non-nucleoside inhibitors of hepatitis B virus (HBV) with IC50 ≤0.01 μg/mL in a reverse transcriptase inhibitory effect. And they showed the low toxicity compared with the nucleoside analogues. [Copyright &y& Elsevier]

Polyhexamethylene guanidine phosphate (PHMG-P) has effective antimicrobial activity against various microorganisms and has been widely used as a biocide in commercial products. However, its use as a humidifier disinfectant has provoked fatal idiopathic lung disease in South Korea, especially in pregnant or postpartum women and their young children. PHMG-P-related toxicological studies of reproduction and development in experimental animals have not been identified, and thus, we investigated the potential effects of early-stage oral exposure to PHMG-P by assessing its toxicological properties. PHMG-P was repeatedly administered by oral gavage at dose levels of 0, 13, 40 and 120 mg/kg to Sprague-Dawley rats during the pre-mating, mating, gestation and early lactation periods, and then general systemic and reproductive/developmental toxicities were investigated. At 120 mg/kg, PHMG-P-related toxicities including subdued behavior, thin appearance, decreased body weight, decreased food consumption and decreased F1 pup body weight were observed. Based on the results of this study, the no-observed-adverse-effect levels (NOAELs) of PHMG-P for both general systemic effects and development are considered to be 40 mg/kg/day. • PHMG-P was evaluated in GLP-compliant reproductive and developmental toxicity screening study. • PHMG-P-related toxicities were observed in F0 parental animals and F1 pups at 120 mg/kg/day. • The NOAELs of PHMG-P for both general systemic effects and development are considered to be 40 mg/kg/day. [ABSTRACT FROM AUTHOR]

Background: We sought to assess the prognostic significance of lymph node ratio (LNR) and N stage in patients undergoing D2 gastrectomy and adjuvant chemotherapy, S-1, and XELOX and to compare the efficacy of them according to LNRs and N stages to evaluate the clinical impact of using LNRs compared with using N staging.Methods: Patients undergoing D2 gastrectomy with adequate lymph node dissection and adjuvant chemotherapy for stage II/III gastric cancer between Mar 2011 and Dec 2016 were analysed. Of the 477 patients enrolled, 331 received S-1 and 146 received XELOX. LNR groups were segregated as 0, 0-0.1, 0.1-0.25, and > 0.25 (LNR0, 1, 2, and 3, respectively). Propensity score matching (PSM) was used to minimise potential selection bias and compare DFS and OS stratified by LNRs and N stages in the two treatment groups.Results: After PSM, the sample size of each group was 110 patients, and variables were well balanced. All patients had more than 15 examined lymph nodes (median 51, range 16~124). In multivariate analysis, LNR (> 0.25) and N stage (N3) showed independent prognostic value in OS and DFS, but LNR (> 0.25) showed better prognostic value. In subgroup analysis, the LNR3 group showed better 5-year DFS (20% vs 54%; HR 0.29; p = 0.004) and 5-year OS (26% vs 67%; HR 0.28; p = 0.020) in the XELOX group. The N3 group showed better 5-year DFS (38% vs 66%; HR 0.40; p = 0.004) and 5-year OS (47% vs 71%; HR 0.45; p = 0.019) in the XELOX group. Stage IIIC showed better 5-year DFS (22% vs 57%; HR 0.32; p = 0.004) and 5-year OS (27% vs 68%; HR 0.32; p = 0.009) in the XELOX group. The LNR3 group within N3 patients showed better 5-year DFS (21% vs 55%; HR 0.31; p = 0.004) and 5-year OS (27% vs 68%; HR 0.34; p = 0.018) in the XELOX group.Conclusions: LNR showed better prognostic value than N staging. LNR3, N3 and stage IIIC groups showed the superior efficacy of XELOX to that of S-1. And the LNR3 group within N3 patients showed more survival benefit from XELOX. LNR > 0.25, N3 stage and stage IIIC were the discriminant factors for selecting XELOX over S-1.Trial Registration: Not applicable (retrospective study). [ABSTRACT FROM AUTHOR]

This research examines how organizational error management culture influences organizational commitment and employee turnover intention through employee psychological safety and perceived fairness. Data was collected from 173 hotel employees in Hong Kong. Using structural equation modeling, this study found that (1) error management culture positively influences employee psychological safety and perceived fairness; (2) error management culture had significant indirect effect on employee' s turnover intentions, sequentially mediated by perceived fairness, psychological safety and organization commitment. [ABSTRACT FROM AUTHOR]

An attempt has been made to use an instrumented TUG (iTUG) that complements the limitations of the traditional "timed up and go" (TUG). However, the variables that represent the characteristics of a faller have been reported to be different among preceding studies with iTUG. Thus, the purpose of this study was to develop a fall prediction model based on three years follow-up study with iTUG. Total 69 subjects participated in this experiment: 26 fallers (4 male and 22 female) who fell within 12 months from the first year measurement date and those newly fallen within 12 months from the second and third year measurement day were added up, and 43 non-fallers (11 male and 32 female) who had no falls. ITUG was performed once a year (two experiments per year) for three consecutive years using IMU sensor system (APDM Inc.). Among 30 variables, the final fall prediction model with logistic regression analysis consisted of five variables such as the duration of the total and the sit-to-stand phase, peak velocity of trunk sagittal plane and range of motion of trunk horizontal plane during gait phase and peak turn velocity during the turn-to-sit phase. Prediction accuracy using the receiver operation characteristic curve was 69.9 %. It is necessary to develop a more accurate fall prediction model by increasing the follow-up period and adding the numbers of the fallers. Further, it is important to identify meaningful variables by consecutive years rather than simple annual comparison. [ABSTRACT FROM AUTHOR]

This study investigates technology affordances of online travel media determining ways of presenting pictorial information and creating the spatial structure of a destination: modality and navigability. To examine the impacts of technology affordances on virtual travel experience, a 2 (modality: still pictures versus panoramic pictures) × 2 (navigability: absence versus presence) between-subjects experiment was conducted with 213 participants. This study found significant effects of modality and navigability on affective and cognitive dimensions of virtual travel experience. Scrutinizing the mediating role of virtual travel experience, the findings explain the psychological mechanism of how modality and navigability influence tourists’ behavioral intention. [ABSTRACT FROM PUBLISHER]

Toluene is an organic solvent that is used in various industrial applications. Despite its usefulness, toluene has toxic effects on the brain and is a substance that is commonly abused. Toluene causes behavioral and functional abnormalities such as decreased memory capacity, cognitive impairment, and depression-like symptoms. However, the target sites and toxic mechanisms of inhaled toluene in the brain are poorly understood. In this study, we subjected Sprague-Dawley (SD) rats to acute high-level toluene exposure (7000 ppm) to investigate its neuronal toxicity, and in particular, its effect on neurogenesis in the hippocampus. In order to assay the effects of inhaled toluene on hippocampal neurogenesis, we measured the levels of neurogenesis markers Ki-67 and doublecortin (DCX) in the hippocampus 1, 2, 5, and 8 days after cessation of toluene exposure. In addition to assaying clinical signs, body weight, and bronchoalveolar lavage fluid, the liver, lungs, and kidneys were subjected to histopathological examination to investigate the toxic effects of high-level toluene exposure. Although abnormal neurological signs were observed after toluene exposure, these disappeared within 24 h and no toluene-related toxicological effects were observed in the liver, lungs, or kidneys. The animals exposed to toluene showed significantly decreased hippocampal neurogenesis, which persisted until the 8th and final day of measurement. Thus, acute high-level toluene exposure inhibited hippocampal neurogenesis and produced transient abnormal neurological signs, but did not produce toxicity in the other organs studied. [ABSTRACT FROM AUTHOR]

Although the potential risk of silver nanoparticles (AgNPs) to humans has recently increased due to widespread application, the potential effects of AgNPs on embryo-fetal development have not yet been determined. This study investigated the potential effects of AgNPs on pregnant dams and embryo-fetal development after maternal exposure on gestational days (GD) 6-19 in rats. AgNPs were administered to pregnant rats by gavage at concentrations of 0, 100, 300, and 1000 mg/kg/day. All dams were subjected to Cesarean section on GD 20 and the fetuses were examined for signs of embryotoxic and teratogenic effects. Examinations of hepatic oxidant/antioxidant balance and serum biochemistry were also added to the routine developmental toxicity study. Treatment with AgNPs caused a decrease in catalase and glutathione reductase activities at ≥100 mg/kg/day and a reduction in glutathione content at 1000 mg/kg/day in maternal liver tissues. However, no treatment-related deaths or clinical signs were observed in any of the animals treated with AgNPs. No treatment-related differences in maternal body weight, food consumption, gross findings, serum biochemistry, organ weight, gestation index, fetal deaths, fetal and placental weights, sex ratio, or morphological alterations were observed between the groups. The results show that repeated oral doses of AgNPs during pregnancy caused oxidative stress in hepatic tissues at ≥100 mg/kg/day, but did not cause developmental toxicity at doses of up to 1000 mg/kg/day. The no-observed-adverse-effect level of AgNPs is considered to be <100 mg/kg/day for dams and 1000 mg/kg/day for embryo-fetal development. [ABSTRACT FROM AUTHOR]

Acetogenic bacteria demonstrate industrial potential for utilizing carbon dioxide (CO 2) for biochemical production using the Wood-Ljungdahl pathway. However, the metabolic engineering of acetogenic bacteria has been hampered by the limited number of available genetic bioparts for gene expression. Here, we integrated RNA sequencing, ribosome profiling, differential RNA sequencing, and RNA 3′-end sequencing results of Eubacterium limosum to establish genetic bioparts, such as promoters, 5′ untranslated regions, and transcript terminators, to regulate transcriptional and translational expression of genes composing of biosynthetic pathways. In addition, a transformation method for the strain was developed to efficiently deliver the obtained genetic bioparts into cells, resulting in a transformation efficiency of 2.5 × 105 CFU/μg DNA. Using this method, the genetic bioparts were efficiently introduced, and their strengths were measured, which were then applied to optimize the heterologous expression of acetolactate synthase and acetolactate decarboxylase for non-native biochemical acetoin production. The strategy developed in this study is the first report on integrating multi-omics data for biopart development of CO 2 or syngas utilizing acetogenic bacteria, which lays a foundation for the efficient production of biochemicals from CO 2 or syngas as a carbon feedstock under autotrophic growth conditions. • Development of genetic tools for CO2-fixing Eubacterium limosum. • Unraveling transcript architecture using transcript start sites and 3՛ end sites. • Utilizing transcript 3՛ end position as metabolic valves to regulate gene expression. • Autotrophic acetoin production using bioparts designed by the multi-omics dataset. [ABSTRACT FROM AUTHOR]

Abstract: We present a method to pattern solution-processed oxide semiconductor thin films by all laser process. A metal thin film is first photoetched by a spatially-modulated pulsed Nd–YAG laser beam and this layer is then covered with a semiconductor film. Uniform irradiation by the same laser generates a thermo-elastic force on the underlying metal layer and this force serves to detach it from the substrate, leaving only a patterned semiconductor structure. Sharp-edged zinc–tin oxide (ZTO) patterns at the micrometer scales could be fabricated over a few square centimeters by a single pulse of 850mJ. A mobility of 7.6×10−2 cm2 V−1 s−1, an on/off ratio higher than 106, and an off-current of 1.91×10−11 A were achieved from a thin film transistor (TFT) with the patterned ZTO channel. These values were similar to those from a reference TFT, demonstrating the feasibility of this patterning process for electronic devices. [Copyright &y& Elsevier]

This study's main objective is to estimate the economic impact of a mega-convention, including an exhibition that is scheduled to be held in May 2016 in Korea. This study uses the multiplier effect of an input-output model to estimate the economic impact of hosting the convention. To compute the mega-convention's total expenditures, four sources are considered: conference hosts, exhibition host, exhibitors, and foreign attendees. The total expenditures by the four sources generate US$70.2 million. The total convention receipts of US$70.2 million produce US$132.8 million in output, 1,320 full-time-equivalent jobs, US$23.9 million in personal income for residents, US$58.4 million in value added, US$8.1 million in indirect tax, and US$11.1 million in imports. [ABSTRACT FROM AUTHOR]

Bisphenol F (BPF, 4,4′-methylenediphenol) has recently been selected as an alternative to bisphenol A (BPA), which is used in the manufacturing of polycarbonates and epoxy resins. This study aimed to investigate the general, and reproductive/developmental effects of BPF. Therefore, BPF at dose levels of 0, 1, 5, 20, and 100 mg/kg/day was administered daily by oral gavage to Sprague-Dawley rats during the pre-mating, mating, gestation, and early lactation periods, and reproductive and developmental toxicities including general systemic toxicities were investigated. A decrease in body weight and food consumption was observed in the female rats treated with BPF at 20 and 100 mg/kg/day during the pre-mating and gestation periods. Additionally, gamma glutamyl transpeptidase levels were increased in the female rats administered 100 mg/kg/day. At 100 mg/kg/day, ovarian weight decreased and vaginal mucification increased according to a necropsy and histopathological examination, respectively. Moreover, the number of implantation sites and litter size decreased at 100 mg/kg/day. However, no significant BPF-related changes were observed in the male rats. Based on the results of this study, the no-observed-adverse-effect levels (NOAELs) of BPF for general systemic and reproductive effects were 5 and 20 mg/kg/day, respectively. • A bisphenol F(BPF)-related decrease in body weight and food consumption was observed in female rats. • BPF decreased gamma glutamyl transpeptidase levels and ovarian weight, and increased vaginal mucification. • BPF treatment induced a decrease in the number of implantation sites and litter size. • The NOAELs were considered 5 and 20 mg/kg/day for general systemic and reproductive/developmental effects, respectively. [ABSTRACT FROM AUTHOR]

Sore throat lozenges, which are over-the-counter drugs, contain 2,4-dichlorobenzyl alcohol (DCBA) as the primary ingredient. However, comprehensive data on the prenatal developmental toxicity of DCBA is limited. Therefore, this study was conducted to determine the effects of DCBA on pregnant rats and prenatal development. Sprague-Dawley rats were administered different doses of DCBA (0, 25, 100, 400, and 800 mg/kg/day) daily via an oral gavage from gestation day (GD) 6–19. Thereafter, all the live dams were sacrificed on GD 20, and caesarean sections were conducted. Live fetuses and their placenta were weighed and then examined for external, visceral, and skeletal malformations and variations. Based on the results obtained, dams at 800 mg/kg/day showed systemic toxicities, including a decrease in body weight and food consumption, and liver changes. Additionally, this treatment induced decreases in fetal and placental weights, as well as the increased incidence of retarded ossifications and full supernumery rib, and the decreased number of ossification centers. Therefore, based on these findings, the no-observed-adverse-effect level of DCBA was determined to be 400 mg/kg/day for dams and prenatal development. • Prenatal development toxicities of 2,4-dichlorobenzyl alcohol (DCBA) were evaluated. • A DCBA dose of 800 mg/kg/day induced systemic toxicities on pregnant rats. • The 800 mg/kg/day DCBA treatment also decreased fetal and placental weights. • This DCBA dose increased the incidence of retarded ossification-related findings and full supernumerary rib. • The no-observed-adverse-effect level of DCBA was determined to be 400 mg/kg/day. [ABSTRACT FROM AUTHOR]

Abstract: Antitumor effects of a ginsenoside Rg3-fortified red ginseng preparation (Rg3-RGP) were investigated in human non-small cell lung carcinoma (H460) cells using in vitro cytotoxicity assay and in vivo nude mouse xenograft model. Immunomodulatory effects of the preparation were also assessed by measuring the facilitating activities on the nitric oxide (NO) release from peritoneal macrophages, in vitro and in vivo lymphocyte proliferation, and the carbon clearance from circulating blood. In a cell level, Rg3-RGP exerted H460 cytotoxicity and facilitated splenocyte proliferation at very high concentrations, without affecting NO production. However, oral administration of Rg3-RGP (100–300mg/kg) enhanced carbon particle-phagocytic index of blood macrophages up to 360–397% of control value. In addition, Rg3-RGP significantly increased the splenocyte proliferation (23% at 100mg/kg). In tumor-bearing mice, 28-day oral treatment with Rg3-RGP (100mg/kg) remarkably suppressed the tumor growth, leading to the decrease of the tumor volume and weight by 30–31%, which was comparable to the effect (27–29% reduction) of doxorubicin (2mg/kg at 3-day intervals). While Rg3-RGP did not cause adverse effects, intravenous injection of doxorubicin markedly decreased body and testes weights, and exhibited severe depletion of spermatogenic cells in the atrophic seminiferous tubules. These results indicate that Rg3-RGP exerts antitumor activities via indirect immunomodulatory actions, without causing adverse effects as seen in doxorubicin. [Copyright &y& Elsevier]