31 results on '"Cohen, G. M."'
Search Results
2. Telemedicine and paediatric obesity treatment: review of the literature and lessons learnt.
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Cohen, G. M., Irby, M. B., Boles, K., Jordan, C., and Skelton, J. A.
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Paediatric obesity is more prevalent in rural areas, yet rural families may not have access to paediatric obesity treatment programmes. The use of new technologies, particularly telemedicine, has proven effective in other behavioural fields, such as psychiatry. This paper reviews the literature on the use of telemedicine in paediatric obesity treatment and describes one tertiary-care paediatric obesity telemedicine programme. We performed a systematic review of the literature from 1990 to 2011 using the following criteria: paediatric age group, overweight or obesity care or treatment, and use of telemedicine technology. Of 2873 abstracts identified, four studies met all inclusion criteria; all were published after 2008. The limited evidence suggests telemedicine to be a promising approach to paediatric weight management, particularly for rural families with limited access to treatments. We also provide important lessons learnt from one paediatric obesity treatment clinic offering services to rural families via telemedicine. Few studies have examined the use of telemedicine for paediatric obesity treatment, but the available data favour this method for treating rural patients. There are several unique key factors influencing successful delivery of a paediatric obesity telemedicine treatment programme. This review identifies a potential avenue for expanded treatment and highlights the need for further investigation. [ABSTRACT FROM AUTHOR]
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- 2012
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3. Anisotropic capillary instability of silicon nanostructures under hydrogen anneal.
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Barwicz, T., Cohen, G. M., Reuter, K. B., Bangsaruntip, S., and Sleight, J. W.
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ANISOTROPY , *SILICON , *NANOSILICON , *HYDROGEN , *NANOWIRES , *NANOSTRUCTURED materials - Abstract
Anneal in reduced pressure hydrogen ambient is known to induce morphological changes in silicon microstructures via markedly increased surface self-diffusivity on exposed silicon surfaces. Here, we investigate the capillary instability of silicon nanostructures under hydrogen anneal. We demonstrate that a surface diffusion mask can significantly improve stability by isolating vulnerable segments from large mass reservoirs. In addition, we find that Plateau-Rayleigh instability shows strong crystallographic dependence, which is explained by the surface energy anisotropy of silicon. We observe that nanowires are the least stable when their axial orientation corresponds to <100> and are increasingly stable for <111>, <112>, and <110>. [ABSTRACT FROM AUTHOR]
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- 2012
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4. Nanowire metal-oxide-semiconductor field effect transistor with doped epitaxial contacts for source and drain.
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Cohen, G. M., Rooks, M. J., Chu, J. O., Laux, S. E., Solomon, P. M., Ott, J. A., Miller, R. J., and Haensch, W.
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PHYSICS research , *NANOWIRES , *BORON , *SILICON , *SEMICONDUCTORS , *QUANTUM wells , *METAL semiconductor field-effect transistors - Abstract
The authors report the fabrication of a p-field effect transistor (FET) and an n-FET with a silicon nanowire channel and doped silicon source and drain regions. The silicon nanowires were synthesized by the vapor-liquid-solid method. For p-FETs the source and drain regions were formed by adding boron doped silicon to the unintentionally doped nanowire body at predefined locations using in situ doped silicon epitaxy. For n-FETs the epitaxial source and drain regions were grown undoped and were later implanted with P and As. The measured Id-Vg characteristics of the devices exhibited unipolar transport, while reference FETs made with nanowires from the same batch but with Schottky (metal) contacts exhibited ambipolar characteristics. [ABSTRACT FROM AUTHOR]
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- 2007
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5. Dislocation-free strained silicon-on-silicon by in-place bonding.
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Cohen, G. M., Mooney, P. M., Paruchuri, V. K., and Hovel, H. J.
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SILICON-on-insulator technology , *ELECTRIC insulators & insulation , *SEMICONDUCTORS , *DISLOCATIONS in crystals , *CRYSTALLOGRAPHY , *DEFORMATIONS (Mechanics) , *VAN der Waals forces , *QUASIMOLECULES , *POLARIZATION (Electricity) - Abstract
In-place bonding is a technique where silicon-on-insulator (SOI) slabs are bonded by hydrophobic attraction to the underlying silicon substrate when the buried oxide is undercut in dilute HF. The bonding between the exposed surfaces of the SOI slab and the substrate propagates simultaneously with the buried oxide etching. As a result, the slabs maintain their registration and are referred to as “bonded in-place”. We report the fabrication of dislocation-free strained silicon slabs from pseudomorphic trilayer Si/SiGe/SOI by in-place bonding. Removal of the buried oxide allows the compressively strained SiGe film to relax elastically and induce tensile strain in the top and bottom silicon films. The slabs remain bonded to the substrate by van der Waals forces when the wafer is dried. Subsequent annealing forms a covalent bond such that when the upper Si and the SiGe layer are removed, the bonded silicon slab remains strained. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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6. Death receptors leave a caspase footprint that Smacs of XIAP.
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Bratton, S B and Cohen, G M
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MITOCHONDRIA , *APOPTOSIS - Abstract
Cell Death and Differentiation (2003) 10, 4-6. doi:10.1038/sj.cdd.4401176 [ABSTRACT FROM AUTHOR]
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- 2003
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7. Ion channeling and x-ray diffraction study of tensely strained GaInP layers on InP.
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Cohen, G. M. and Ritter, D.
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THIN films , *ION channels - Abstract
Presents an ion channeling and x-ray diffraction study of tensely strained GaInP layers on InP. Angular difference between the substrate and the layer channeling angles; Negligible relaxation.
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- 1999
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8. Strain compensated InGaAs/InGaP quantum well infrared detector for midwavelength band detection.
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Maimon, S., Cohen, G. M., Finkman, E., Bahir, G., Ritter, D., and Schacham, S. E.
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QUANTUM wells , *INFRARED detectors , *MOLECULAR beam epitaxy , *SCIENCE - Abstract
Examines the strain compensated InGaAs/InGaP quantum well infrared detector for midwavelength band detection. Usability of bound-to-continuum intersubband absorption; Implementation of photodetectors with background-limited performance with detectivity; Importance of metal organic molecular beam epitaxy on a semi-insulating InP substrate.
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- 1998
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9. Kinetics of nickel silicide growth in silicon nanowires: From linear to square root growth.
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Yaish, Y. E., Katsman, A., Cohen, G. M., and Beregovsky, M.
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SILICIDES , *NANOWIRES , *NICKEL , *SCANNING electron microscopy , *TRANSMISSION electron microscopy - Abstract
The common practice for nickel silicide formation in silicon nanowires (SiNWs) relies on axial growth of silicide along the wire that is initiated from nickel reservoirs at the source and drain contacts. In the present work the silicide intrusions were studied for various parameters including wire diameter (25-50 nm), annealing time (15-120 s), annealing temperature (300-440°C), and the quality of the initial Ni/Si interface. The silicide formation was investigated by high-resolution scanning electron microscopy, high-resolution transmission electron microscopy (TEM), and atomic force microscopy. The main part of the intrusion formed at 420°C consists of monosilicide NiSi, as was confirmed by energy dispersive spectroscopy STEM, selected area diffraction TEM, and electrical resistance measurements of fully silicided SiNWs. The kinetics of nickel silicide axial growth in the SiNWs was analyzed in the framework of a diffusion model through constrictions. The model calculates the time dependence of the intrusion length, L, and predicts crossover from linear to square root time dependency for different wire parameters, as confirmed by the experimental data. [ABSTRACT FROM AUTHOR]
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- 2011
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10. Caspase inactivation of the proteasome.
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Cohen, G. M.
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UBIQUITIN , *APOPTOSIS , *CELL death , *PROTEINS - Abstract
Discusses a study which unraveled a novel interaction between the ubiquitin proteasome system (UPS) and caspases during apoptotic cell death. Concentration of almost all previous studies on how the UPS system regulated apoptosis by controlling the degradation of key apoptotic regulators; Data showing how key events in apoptosis, namely the activation of caspases, can modulate the function of the proteasome; Caspase-mediated cleavage of the proteasomal subunits which resulted in an inhibition of both ubiquitin dependent and -independent substrates.
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- 2005
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11. Pharmaceutical branding of resident physicians.
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Sigworth, S K, Nettleman, M D, and Cohen, G M
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INDUSTRIES , *INTERNSHIP programs , *INTERPROFESSIONAL relations , *GIFT giving - Published
- 2001
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12. The transrepression arm of glucocorticoid receptor signaling is protective in mutant huntingtin-mediated neurodegeneration.
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Varadarajan, S, Breda, C, Smalley, J L, Butterworth, M, Farrow, S N, Giorgini, F, and Cohen, G M
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GLUCOCORTICOID receptors , *HUNTINGTIN protein , *NEURODEGENERATION , *ENDOPLASMIC reticulum , *HOMEOSTASIS , *CELL death - Abstract
The unfolded protein response (UPR) occurs following the accumulation of unfolded proteins in the endoplasmic reticulum (ER) and orchestrates an intricate balance between its prosurvival and apoptotic arms to restore cellular homeostasis and integrity. However, in certain neurodegenerative diseases, the apoptotic arm of the UPR is enhanced, resulting in excessive neuronal cell death and disease progression, both of which can be overcome by modulating the UPR. Here, we describe a novel crosstalk between glucocorticoid receptor signaling and the apoptotic arm of the UPR, thus highlighting the potential of glucocorticoid therapy in treating neurodegenerative diseases. Several glucocorticoids, but not mineralocorticoids, selectively antagonize ER stress-induced apoptosis in a manner that is downstream of and/or independent of the conventional UPR pathways. Using GRT10, a novel selective pharmacological modulator of glucocorticoid signaling, we describe the importance of the transrepression arm of the glucocorticoid signaling pathway in protection against ER stress-induced apoptosis. Furthermore, we also observe the protective effects of glucocorticoids in vivo in a Drosophila model of Huntington's disease (HD), wherein treatment with different glucocorticoids diminished rhabdomere loss and conferred neuroprotection. Finally, we find that growth differentiation factor 15 has an important role downstream of glucocorticoid signaling in antagonizing ER stress-induced apoptosis in cells, as well as in preventing HD-mediated neurodegeneration in flies. Thus, our studies demonstrate that this novel crosstalk has the potential to be effectively exploited in alleviating several neurodegenerative disorders. [ABSTRACT FROM AUTHOR]
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- 2015
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13. Evaluation and critical assessment of putative MCL-1 inhibitors.
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Varadarajan, S, Vogler, M, Butterworth, M, Dinsdale, D, Walensky, L D, and Cohen, G M
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MCL1 protein , *CANCER relapse , *APOPTOSIS , *TARGETED drug delivery , *CANCER treatment - Abstract
High levels of BCL-2 family proteins are implicated in a failed/ineffective apoptotic programme, often resulting in diseases, including cancer. Owing to their potential as drug targets in cancer therapy, several inhibitors of BCL-2 family proteins have been developed. These primarily target specific members of the BCL-2 family, particularly BCL-2 and BCL-XL but are ineffective against MCL-1. Major efforts have been invested in developing inhibitors of MCL-1, which is commonly amplified in human tumours and associated with tumour relapse and chemoresistance. In this report, the specificity of several BCL-2 family inhibitors (ABT-263, UCB-1350883, apogossypol and BH3I-1) was investigated and compared with putative MCL-1 inhibitors designed to exhibit improved or selective binding affinities for MCL-1 (TW-37, BI97C1, BI97C10, BI112D1, compounds 6 and 7, and MCL-1 inhibitor molecule (MIM-1)). ABT-263, BI97C1, BI112D1, MIM-1 and TW-37 exhibited specificity in inducing apoptosis in a Bax/Bak- and caspase-9-dependent manner, whereas the other agents showed no killing activity, or little or no specificity. Of these inhibitors, only ABT-263 and UCB-1350883 induced apoptosis in a BCL-2- or BCL-XL-dependent system. In cells that depend on MCL-1 for survival, ABT-263 and TW-37 induced extensive apoptosis, suggesting that at high concentrations these inhibitors have the propensity to inhibit MCL-1 in a cellular context. TW-37 induced apoptosis, assessed by chromatin condensation, caspase processing and phosphatidylserine externalisation, in a BAK-dependent manner and in cells that require MCL-1 for survival. TW-37-mediated apoptosis was also partly dependent on NOXA, suggesting that derivatives of TW-37, if engineered to exhibit better selectivity and efficacy at low nanomolar concentrations, may provide useful lead compounds for further synthetic programmes. Expanded medicinal chemistry iteration, as performed for the ABT series, may likewise improve the potency and specificity of the evaluated MCL-1 inhibitors. [ABSTRACT FROM AUTHOR]
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- 2013
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14. A novel cellular stress response characterised by a rapid reorganisation of membranes of the endoplasmic reticulum.
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Varadarajan, S, Bampton, E T W, Smalley, J L, Tanaka, K, Caves, R E, Butterworth, M, Wei, J, Pellecchia, M, Mitcheson, J, Gant, T W, Dinsdale, D, and Cohen, G M
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ENDOPLASMIC reticulum , *CELL membranes , *MEMBRANE proteins , *ANTIPSYCHOTIC agents , *ANTIMALARIALS - Abstract
Canonical endoplasmic reticulum (ER) stress, which occurs in many physiological and disease processes, results in activation of the unfolded protein response (UPR). We now describe a new, evolutionarily conserved cellular stress response characterised by a striking, but reversible, reorganisation of ER membranes that occurs independently of the UPR, resulting in impaired ER transport and function. This reorganisation is characterised by a dramatic redistribution and clustering of ER membrane proteins. ER membrane aggregation is regulated, in part, by anti-apoptotic BCL-2 family members, particularly MCL-1. Using connectivity mapping, we report the widespread occurrence of this stress response by identifying several structurally diverse chemicals from different pharmacological classes, including antihistamines, antimalarials and antipsychotics, which induce ER membrane reorganisation. Furthermore, we demonstrate the potential of ER membrane aggregation to result in pathological consequences, such as the long-QT syndrome, a cardiac arrhythmic abnormality, arising because of a novel trafficking defect of the human ether-a-go-go-related channel protein from the ER to the plasma membrane. Thus, ER membrane reorganisation is a feature of a new cellular stress pathway, clearly distinct from the UPR, with important consequences affecting the normal functioning of the ER. [ABSTRACT FROM AUTHOR]
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- 2012
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15. NOXA, a sensor of proteasome integrity, is degraded by 26S proteasomes by an ubiquitin-independent pathway that is blocked by MCL-1.
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Craxton, A, Butterworth, M, Harper, N, Fairall, L, Schwabe, J, Ciechanover, A, and Cohen, G M
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PROTEASOMES , *UBIQUITIN , *PROTEOLYSIS , *APOPTOSIS , *MYELOID leukemia - Abstract
Ubiquitin (Ub)-mediated proteasome-dependent proteolysis is critical in regulating multiple biological processes including apoptosis. We show that the unstructured BH3-only protein, NOXA, is degraded by an Ub-independent mechanism requiring 19S regulatory particle (RP) subunits of the 26S proteasome, highlighting the possibility that other unstructured proteins reported to be degraded by 20S proteasomes in vitro may be bona fide 26S proteasome substrates in vivo. A lysine-less NOXA (NOXA-LL) mutant, which is not ubiquitinated, is degraded at a similar rate to wild-type NOXA. Myeloid cell leukemia 1, but not other anti-apoptotic BCL-2 family proteins, stabilizes NOXA by interaction with the NOXA BH3 domain. Depletion of 19S RP subunits, but not alternate proteasome activator REG subunits, increases NOXA half-life in vivo. A NOXA-LL mutant, which is not ubiquitinated, also requires an intact 26S proteasome for degradation. Depletion of the 19S non-ATPase subunit, PSMD1 induces NOXA-dependent apoptosis. Thus, disruption of 26S proteasome function by various mechanisms triggers the rapid accumulation of NOXA and subsequent cell death strongly implicating NOXA as a sensor of 26S proteasome integrity. [ABSTRACT FROM AUTHOR]
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- 2012
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16. Ordering of caspases in cells undergoing apoptosis by the intrinsic pathway.
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Inoue, S., Browne, G., Melino, G., and Cohen, G. M.
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APOPTOSIS , *CYSTEINE proteinases , *MITOCHONDRIAL DNA , *CYTOPLASM , *PROTEOLYTIC enzymes , *RNA , *NUCLEIC acids - Abstract
Caspases are a family of aspartate-specific cysteine proteases responsible for the biochemical and morphological changes that occur during the execution phase of apoptosis. The hierarchical ordering of caspases has been clearly established using dATP-activated cell lysates to model the intrinsic pathway induced by initial mitochondrial perturbation. In this model, caspase-9, the apical caspase, directly processes and activates the effector caspases, caspase-3 and -7, and then active caspase-3 but not caspase-7, processes caspase-2 and -6, and subsequently the activated caspase-6 processes caspase-8 and -10. To address the possibility that this model in vitro system might not reflect the precise ordering of caspases in intact cells, we have examined this possibility in cells induced to undergo apoptosis by activation of the intrinsic pathway. We have used caspase deficient cells, small interference RNA for caspase-6 and -7, and a specific caspase-3 inhibitor. In contrast to the earlier in vitro studies, we now show that in intact cells caspase-7 can also directly process and activate caspase-2 and -6. The processing of caspase-2 and -6 occurs within the cytoplasm and active caspase-6 is then responsible for both the processing of caspase-8 and the cleavage of caspase-6 substrates, including lamin A/C.Cell Death and Differentiation (2009) 16, 1053–1061; doi:10.1038/cdd.2009.29; published online 27 March 2009 [ABSTRACT FROM AUTHOR]
- Published
- 2009
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17. Different forms of cell death induced by putative BCL2 inhibitors.
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Vogler, M., Weber, K., Dinsdale, D., Schmitz, I., Schulze-Osthoff, K., Dyer, M. J. S., and Cohen, G. M.
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CELL death , *ENZYME inhibitors , *B cell lymphoma , *CANCER cells , *CANCER treatment , *CYTOCHROME c , *CYTOCHROMES , *APOPTOSIS - Abstract
Several inhibitors of BCL2 proteins have been identified that induce apoptosis in a variety of tumor cells, indicating their potential in cancer therapy. We investigated the specificity of six putative BCL2 inhibitors (obatoclax, gossypol, apogossypol, EM20-25, chelerythrine and ABT-737). Using cells deficient either for Bax/Bak or caspase-9, we found that only ABT-737 specifically targeted BCL2 proteins and induced apoptosis by activation of caspase-9, as only ABT-737 induced apoptosis was completely inhibited in cells deficient for Bax/Bak or caspase-9. Our data show that only ABT-737 is a specific BCL2 inhibitor and all other compounds investigated were not specific for BCL2 proteins. Furthermore, investigations of the effects of these compounds in primary chronic lymphocytic leukemic cells showed that all compounds induced certain biochemical hallmarks of apoptosis, such as release of cytochrome c and caspase cleavage. However, they all caused strikingly different ultrastructural changes. ABT-737 induced all the characteristic ultrastructural changes of apoptosis together with early rupture of the outer mitochondrial membrane, whereas obatoclax, chlelerythrine and gossypol induced pronounced mitochondrial swelling with formation of phospholipid inclusions. Therefore, we conclude that biochemical measurements used earlier to define apoptosis like mitochondrial release of cytochrome c and caspase cleavage, are insufficient to distinguish between classic apoptosis and other forms of cell death.Cell Death and Differentiation (2009) 16, 1030–1039; doi:10.1038/cdd.2009.48; published online 24 April 2009 [ABSTRACT FROM AUTHOR]
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- 2009
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18. Bcl-2 inhibitors: small molecules with a big impact on cancer therapy.
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Vogler, M., Dinsdale, D., Dyer, M. J. S., and Cohen, G. M.
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APOPTOSIS , *CANCER treatment , *CELL death , *MOLECULES , *B cells - Abstract
Despite tremendous advances over the last 15 years in understanding fundamental mechanisms of apoptosis, this has failed to translate into improved cancer therapy for patients. However, there may now be light at the end of this long tunnel. Antiapoptotic Bcl-2 family members may be divided into two subclasses, one comprising Bcl-2, Bcl-XL and Bcl-w and the other Mcl-1 and Bcl2A1. Neutralization of both subclasses is required for apoptosis induction. Solution of the structure of antiapoptotic Bcl-2 family proteins has led to the design of novel small molecule inhibitors. Although many such molecules have been synthesized, rigorous verification of their specificity has often been lacking. Further studies have revealed that many putative Bcl-2 inhibitors are not specific and have other cellular targets, resulting in non-mechanism based toxicity. Two notable exceptions are ABT-737 and a related orally active derivative, ABT-263, which bind with high affinity to Bcl-2, Bcl-XL and Bcl-w and may prove to be useful tools for mechanistic studies. ABT-263 is in early clinical trials in lymphoid malignancies, small-cell lung cancer and chronic lymphocytic leukemia, and some patients have shown promising results. In in vitro studies, primary cells from patients with various B-cell malignancies are exquisitely sensitive to ABT-737, exhibiting novel morphological features of apoptosis including marked outer mitochondrial membrane rupture.Cell Death and Differentiation (2009) 16, 360–367; doi:10.1038/cdd.2008.137; published online 19 September 2008 [ABSTRACT FROM AUTHOR]
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- 2009
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19. p73 and caspase-cleaved p73 fragments localize to mitochondria and augment TRAIL-induced apoptosis.
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Sayan, A E, Sayan, B S, Gogvadze, V, Dinsdale, D, Nyman, U, Hansen, T M, Zhivotovsky, B, Cohen, G M, Knight, R A, and Melino, G
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PROTEIN research , *MITOCHONDRIA , *TUMOR necrosis factors , *APOPTOSIS , *GENETIC transcription , *LIGAND binding (Biochemistry) - Abstract
The p73 protein, a member of the p53 family, has both developmental and tumorigenic functions. Here we show that p73 is cleaved by caspase-3 and -8 both in vitro and in vivo during apoptosis elicited by DNA-damaging drugs and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor ligation. TAp73 and some of its cleavage products are localized to mitochondria. siRNA-mediated downregulation of p73 expression induced a small but significant change in the susceptibility of HCT116 cells to TRAIL-induced apoptosis. A transcription-deficient mutant of TAp73 enhanced TRAIL-induced apoptosis suggesting that p73 protein has transcription-independent functions during death receptor-mediated apoptosis. Additionally, recombinant p73 protein induced cytochrome c release from isolated mitochondria providing evidence that nonnuclear p73 may have additional functions in the progression of apoptosis.Oncogene (2008) 27, 4363–4372; doi:10.1038/onc.2008.64; published online 24 March 2008 [ABSTRACT FROM AUTHOR]
- Published
- 2008
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20. A novel paradigm for rapid ABT-737-induced apoptosis involving outer mitochondrial membrane rupture in primary leukemia and lymphoma cells.
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Vogler, M., Dinsdale, D., Sun, X.-M., Young, K. W., Butterworth, M., Nicotera, P., Dyer, M. J. S., and Cohen, G. M.
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APOPTOSIS , *CELL death , *CELL differentiation , *CHRONIC lymphocytic leukemia , *MITOCHONDRIAL membranes , *LEUKEMIA , *CHRONIC diseases - Abstract
Primary chronic lymphocytic leukemia (CLL) cells are exquisitely sensitive to ABT-737, a small molecule BCL2-antagonist, which induces many of the classical biochemical and ultrastructural features of apoptosis, including BAX/BAK oligomerization, cytochrome c release, caspase activation and chromatin condensation. Surprisingly, ABT-737 also induces mitochondrial inner membrane permeabilization (MIMP) resulting in mitochondrial matrix swelling and rupture of the outer mitochondrial membrane (OMM), so permitting the rapid efflux of cytochrome c from mitochondrial cristae and facilitating rapid caspase activation and apoptosis. BAX and BAK appear to be involved in the OMM discontinuities as they localize to the OMM break points. Notably, ABT-737 induced mitochondrial matrix swelling and OMM discontinuities in other primary B-cell malignancies, including mantle cell, follicular and marginal zone lymphoma cells but not in several cell lines studied. Thus, we describe a new paradigm of apoptosis in primary B-cell malignancies, whereby targeting of BCL2 results in all the classical features of apoptosis together with OMM rupture independent of caspase activation. This mechanism may be far more prevalent than hitherto recognized due to the failure of most methods, used to measure apoptosis, to recognize such a mechanism.Cell Death and Differentiation (2008) 15, 820–830; doi:10.1038/cdd.2008.25; published online 29 February 2008 [ABSTRACT FROM AUTHOR]
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- 2008
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21. Downregulation of Mcl-1 potentiates HDACi-mediated apoptosis in leukemic cells.
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Inoue, S., Walewska, R., Dyer, M. J. S., and Cohen, G. M.
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HISTONE deacetylase , *APOPTOSIS , *CANCER cells , *MESSENGER RNA , *CYCLIN-dependent kinases , *CHRONIC lymphocytic leukemia , *CLINICAL trials , *PROTEIN analysis , *RNA analysis , *BIOCHEMISTRY , *CELL culture , *CELLS , *COMPARATIVE studies , *ENZYME inhibitors , *GENES , *LEUKEMIA , *PHENOMENOLOGY , *RESEARCH methodology , *MEDICAL cooperation , *PROTEINS , *RESEARCH , *RESEARCH funding , *TRANSFERASES , *EVALUATION research , *PHARMACODYNAMICS - Abstract
Mcl-1 is an antiapoptotic Bcl-2 family member, whose degradation is supposedly required for the induction of apoptosis. However, histone deacetylase inhibitors (HDACi) induce apoptosis primarily through the Bak/Mcl-1/Noxa and Bim pathways without decreasing Mcl-1. To investigate this discrepancy, we examined the role of Mcl-1 on HDACi-mediated apoptosis. Inhibition of either class I or class II HDAC by selective HDACi caused an upregulation of Mcl-1 mRNA and protein. Downregulation of Mcl-1 by three structurally unrelated cyclin-dependent kinase inhibitors potentiated HDACi-mediated apoptosis in primary chronic lymphocytic leukemic (CLL) cells and K562 cells. Sensitivity to HDACi-induced apoptosis was increased approximately 10-fold by the cyclin-dependent kinase inhibitors. Nanomolar concentrations of HDACi, approximately 300-fold lower than that required to induce apoptosis alone, sensitized cells to TRAIL, emphasizing that the mechanism(s) whereby HDACi induce apoptosis is clearly distinct from those by which they sensitize to TRAIL. Furthermore, knockdown of Mcl-1-potentiated HDACi-mediated apoptosis in K562 cells. Thus, HDACi-mediated Mcl-1 upregulation plays an important antiapoptotic regulatory role in limiting the efficacy of HDACi-induced apoptosis, which can be overcome by combination with an agent that downregulates Mcl-1. Thus, a clinical trial in some cancers is warranted using a combination of an HDACi with agents that downregulate Mcl-1. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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22. Apoptosis induced by histone deacetylase inhibitors in leukemic cells is mediated by Bim and Noxa.
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Inoue, S., Riley, J., Gant, T. W., Dyer, M. J. S., and Cohen, G. M.
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APOPTOSIS , *CELL death , *HISTONE deacetylase , *LEUKEMIA , *LYMPHOCYTIC leukemia , *CANCER cells , *CANCER treatment - Abstract
Several histone deacetylase inhibitors (HDACi), which have recently entered early clinical trials, exert their anticancer activity in part through the induction of apoptosis although the precise mechanism of this induction is not known. Induction of apoptosis by structurally diverse HDACi in primary cells from patients with chronic lymphocytic leukemia (CLL) and different leukemic cell lines was mediated by the Bcl-2 regulated intrinsic pathway and demonstrated a requirement for de novo protein synthesis. A marked time-dependent induction of the pro-apoptotic BH3-only proteins, Bim, Noxa and Bmf was observed, which preceded the induction of apoptosis. A key role for both Bim and Noxa was proposed in HDACi-mediated apoptosis based on our findings that siRNA for Bim and Noxa but not Bmf largely prevented the HDACi-induced loss in mitochondrial membrane potential, caspase processing and phosphatidylserine externalization. Noxa, induced by HDACi, in CLL cells and tumor cell lines, bound extensively to Mcl-1, a major anti-apoptotic Bcl-2 family member present in CLL cells. Our data strongly suggests that HDACi induce apoptosis primarily through inactivation of anti-apoptotic Bcl-2 family members by increases in Bim and Noxa and highlights these increases as a potential clinical target for CLL/lymphoma therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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23. Chronic lymphocytic leukemic cells exhibit apoptotic signaling via TRAIL-R1.
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MacFarlane, M., Inoue, S., Kohlhaas, S. L., Majid, A., Harper, N., Kennedy, D. B. J., Dyer, M. J. S., and Cohen, G. M.
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APOPTOSIS , *CELL death , *CHRONIC lymphocytic leukemia , *CANCER cells , *LIGANDS (Biochemistry) , *CELL receptors - Abstract
Clinical trials have been initiated with Apo2L/TRAIL (Genentech) and agonistic mAbs to TRAIL receptors, -R1 and -R2 (Human Genome Sciences). The apoptosis-inducing ability of these mAbs and different TRAIL preparations, in the presence or absence of histone deacetylase inhibitors (HDACi), varied markedly against primary chronic lymphocytic leukaemia (CLL) cells and various tumor cell lines, demonstrating an unanticipated preferential apoptotic signaling via either TRAIL-R1 or -R2. Contrary to literature reports that TRAIL-induced apoptosis occurs primarily via signaling through TRAIL-R2, CLL cells, in the presence of HDACi, undergo predominantly TRAIL-R1-mediated apoptosis. Consequently, Apo2L/TRAIL, which signals primarily through TRAIL-R2, is virtually devoid of activity against CLL cells. To maximize therapeutic benefit, it is essential to ascertain whether a primary tumor signals via TRAIL-R1/-R2, prior to initiating therapy. Thus combination of an agonistic TRAIL-R1 Ab and an HDACi, such as the anticonvulsant sodium valproate, could be of value in treating CLL.Cell Death and Differentiation (2005) 12, 773–782. doi:10.1038/sj.cdd.4401649 Published online 29 April 2005 [ABSTRACT FROM AUTHOR]
- Published
- 2005
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24. Increased sensitivity to TRAIL-induced apoptosis occurs during the adenoma to carcinoma transition of colorectal carcinogenesis.
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Hague, A, Hicks, D J, Hasan, F, Smartt, H, Cohen, G M, Paraskeva, C, and MacFarlane, M
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CANCER treatment , *CELL culture , *CARCINOGENESIS , *CELL death , *CANCER cells , *CELL membranes , *CANCER invasiveness , *ADENOMA , *ANIMAL experimentation , *ANTINEOPLASTIC agents , *APOPTOSIS , *CANCER , *CARRIER proteins , *CELL lines , *CELL receptors , *COLON tumors , *COMPARATIVE studies , *ELECTROPHORESIS , *FLOW cytometry , *GLYCOPROTEINS , *RESEARCH methodology , *MEDICAL cooperation , *MICE , *PROTEINS , *RESEARCH , *TUMOR necrosis factors , *WESTERN immunoblotting , *EVALUATION research , *MEMBRANE glycoproteins , *NEOPLASTIC cell transformation ,RECTUM tumors - Abstract
The death ligand TRAIL (Apo2L) has potential for cancer therapy, since tumour cells are thought to be more sensitive than normal cells. We investigated whether sensitivity to TRAIL increases during the adenoma to carcinoma transition of colorectal carcinogenesis. Under the same culture conditions, we compared the extent of TRAIL-induced apoptosis in four premalignant adenoma and three carcinoma cell lines. Although TRAIL induced some apoptosis in adenoma cultures, the carcinoma cell lines were significantly more sensitive (P<0.001). This finding was recapitulated in an in vitro model of tumour progression in which conversion of the adenoma cell line AA/C1 to a tumorigenic phenotype was associated with increased TRAIL sensitivity (P<0.001). Increased TRAIL sensitivity during colorectal carcinogenesis has been previously attributed to changes in the balance between TRAIL receptors TRAIL-R1 and -R2 and "decoy" receptors TRAIL-R3 and -R4 during malignant progression. To address this, cell surface receptor expression was measured by flow cytometry. In summary, during colorectal carcinogenesis, there is a marked increase in sensitivity to TRAIL-induced apoptosis associated with progression from benign to malignant tumour that could be exploited for colon cancer therapy, but alterations in cell surface TRAIL receptor expression may not be the primary reason for this change. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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25. Histone deacetylase inhibitors potentiate TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in lymphoid malignancies.
- Author
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Inoue, S., MacFarlane, M., Harper, N., Wheat, L. M. C., Dyer, M. J. S., and Cohen, G. M.
- Subjects
- *
TUMOR necrosis factors , *APOPTOSIS , *HISTONE deacetylase , *CHRONIC lymphocytic leukemia , *LYMPHOCYTES , *CELL death , *IMMUNE system - Abstract
New therapies are required for chronic lymphocytic leukemia (CLL), an incurable disease characterized by failure of mature lymphocytes to undergo apoptosis. Activation of cell surface death receptors, such as via TRAIL receptor ligation, may provide a novel therapeutic target for various malignancies. However, CLL and other lymphoid malignancies are resistant to TRAIL. We report that low concentrations of histone deacetylase (HDAC) inhibitors, such as depsipeptide, which alone failed to induce apoptosis, markedly sensitize CLL cells and other primary lymphoid malignancies to TRAIL-induced apoptosis. These combinations caused little or no toxicity to normal lymphocytes. HDAC inhibitors sensitized resistant cells to TRAIL-induced apoptosis by facilitating formation of an active death-inducing signalling complex (DISC), leading to the rapid activation of caspase-8. The facilitated DISC formation also occurred in the absence of TRAIL-R2 upregulation. Thus, the combination of HDAC inhibitors and TRAIL may be valuable in the treatment of various hemopoietic malignancies.Cell Death and Differentiation (2004) 11, S193-S206. doi:10.1038/sj.cdd.4401535 [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
26. CDDO induces apoptosis via the intrinsic pathway in lymphoid cells.
- Author
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Inoue, S., Snowden, R. T., Dyer, M. J. S., and Cohen, G. M.
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- *
LYMPHOCYTIC leukemia , *APOPTOSIS , *CELL proliferation , *MITOCHONDRIAL membranes , *CELL death , *CELLS - Abstract
The peroxisome-proliferator-activated receptor (PPAR) gamma agonist, CDDO, is under investigation for use in various malignancies. The mechanisms by which CDDO induces apoptosis are controversial. We have therefore sought to determine these mechanisms using primary chronic lymphocyte leukemic (CLL) cells and Jurkat cell lines with defined apoptotic abnormalities. In these cells, CDDO induced-apoptosis involved caspase-independent loss in mitochondrial membrane potential followed by caspase processing. The pattern of CDDO-induced caspase processing, defined by use of a caspase inhibitor, strongly suggested that caspase-9 was the apical caspase. Moreover, CDDO induced apoptosis in caspase-8 and FADD-deficient but not in Bcl-xL overexpressing Jurkat cells. In CLL cells, CDDO induced an early release of mitochondrial cytochrome c and Smac that preceded apoptosis. Thus, in both cell types, CDDO induced apoptosis primarily by the intrinsic pathway with caspase-9 as the apical caspase. This has important implications in the design of novel agents for the treatment of CLL and other malignancies. [ABSTRACT FROM AUTHOR]
- Published
- 2004
- Full Text
- View/download PDF
27. Macrophage-mediated clearance of cells undergoing caspase-3-independent death.
- Author
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Turner, C, Devitt, A, Parker, K, MacFarlane, M, Giuliano, M, Cohen, G M, and Gregory, C D
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- *
PHAGOCYTOSIS , *CELL death , *CELL membranes , *MACROPHAGES - Abstract
Little is known of the functions of caspases in mediating the surface changes required for phagocytosis of dying cells. Here we investigate the role played by the effector caspase, caspase-3 in this process using the caspase-3-defective MCF-7 breast carcinoma line and derived caspase-3-expressing transfectants. Our results indicate that, while certain typical features of apoptosis induced by etoposide - namely classical morphological changes and the ability to degrade DNA into oligonucleosomal fragments - are caspase-3-dependent, loss of cell adhesion to plastic and the capacity to interact with, and to be phagocytosed by, human monocyte-derived macrophages - both by CD14-dependent and CD14-independent mechanisms - do not require caspase-3. Furthermore, both etoposide-induced caspase-3-positive and -negative MCF-7 cells suppressed proinflammatory cytokine release by macrophages. These results demonstrate directly that cell surface changes that are sufficient for anti-inflammatory clearance by human macrophages can be regulated independently of stereotypical features of the apoptosis programme that require caspase-3.Cell Death and Differentiation (2003) 10, 302-312. doi:10.1038/sj.cdd.4401170 [ABSTRACT FROM AUTHOR]
- Published
- 2003
- Full Text
- View/download PDF
28. Proteasome inhibitor-induced apoptosis of B-chronic lymphocytic leukaemia cells involves cytochrome c release and caspase activation, accompanied by formation of an approximately 700 kDa Apaf-1 containing apoptosome complex.
- Author
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Almond, J B, Snowden, R T, Hunter, A, Dinsdale, D, Cain, K, and Cohen, G M
- Subjects
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LEUKEMIA , *B cells - Abstract
Proteasome inhibitors, including lactacystin and MG132 (carbobenzoxyl-leucinyl-leucinyl-leucinal), potently induce apoptosis in leukaemic B cells from patients with B cell chronic lymphocytic leukaemia (B-CLL). This pro-apoptotic effect occurs in cells from patients at all stages of the disease, including those resistant to conventional chemotherapy, suggesting that proteasome inhibitors may be useful for treatment of B-CLL. Following initial inhibition of proteasomal activity, these agents induce mitochondrial cytochrome c release and caspase-dependent apoptosis, involving cleavage/activation of caspases -2, -3, -7, -8 and -9. Pre-treatment with the cell permeable caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp (OMe)fluoromethyl ketone (Z-VAD.fmk), did not prevent the release of cytochrome c or partial processing of caspase-9 but prevented activation of effector caspases and the induction of apoptosis. These results suggest that the release of cytochrome c is caspase independent and that caspase-9 is the initiator caspase in proteasome inhibitor-induced apoptosis of B-CLL cells. Activation of B-CLL lysates with dATP results in the formation of an approximately 700 kDa caspase-activating apoptosome complex containing Apaf-1. We describe for the first time the formation of a similar approximately 700 kDa caspase-activating apoptosome complex in B-CLL cells induced to undergo apoptosis by proteasome inhibitors. [ABSTRACT FROM AUTHOR]
- Published
- 2001
- Full Text
- View/download PDF
29. Caspase-3 cleaves Apaf-1 into an ∼30 kDa fragment that associates with an inappropriately oligomerized and biologically inactive ∼1.4 MDa apoptosome complex.
- Author
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Bratton, S B, Walker, G, Roberts, D L, Cain, K, and Cohen, G M
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- *
APOPTOSIS , *PROTEOLYTIC enzymes - Abstract
Cytochrome c and dATP/ATP induce oligomerization of Apaf-1 into two distinct apoptosome complexes: an ∼700 kDa complex, which recruits and activates caspases-9, -3 and -7, and an ∼1.4 MDa complex, which recruits and processes caspase-9, but does not efficiently activate effector caspases. While searching for potential inhibitors of the ∼1.4 MDa apoptosome complex, we observed an ∼30 kDa Apaf-1 immunoreactive fragment that was associated exclusively with the inactive complex. We subsequently determined that caspase-3 cleaved Apaf-1 within its CED-4 domain (SVTD[sup 271] ↓S) in both dATP-activated lysates and apoptotic cells to form a prominent ∼30 kDa (p30) N-terminal fragment. Purified recombinant Apaf-1 p30 fragment weakly inhibited dATP-dependent activation of caspase-3 in vitro. However, more importantly, prevention of endogenous formation of the p30 fragment did not stimulate latent effector caspase processing activity in the large complex. Similarly, the possibility that XlAP, an inhibitor of apoptosis protein (lAP), was responsible for the inactivity of the ∼1.4 MDa complex was excluded as immunodepletion of this caspase inhibitor failed to relieve the inhibition. However, selective proteolytic digestion of the ∼1.4 MDa and ∼700 kDa complexes showed that Apaf-1 was present in conformationally distinct forms in these two complexes. Therefore, the inability of the ∼1.4 MDa apoptosome complex to process effector caspases most likely results from inappropriately folded or oligomerized Apaf-1. [ABSTRACT FROM AUTHOR]
- Published
- 2001
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30. Processing/activation of caspases, -3 and -7 and -8 but not caspase-2, in the induction of apoptosis in B-chronic lymphocytic leukemia cells.
- Author
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King, D, Pringle, J H, Hutchinson, M, and Cohen, G M
- Subjects
- *
APOPTOSIS , *CHRONIC lymphocytic leukemia - Abstract
Chlorambucil and prednisolone, two commonly used drugs in the treatment of chronic lymphocytic leukemia (CLL), induce apoptosis in CLL cells. We have investigated the involvement in this apoptotic cell death of caspases, which cleave critical cellular substrates thereby acting as the executioners of the apoptotic process. Induction of spontaneous or chlorambucil/prednisolone-induced apoptosis of freshly isolated B-CLL cells in culture resulted in the activation of the 'effector' caspases, -3 and -7, but generally not of caspase-2. Activation of caspases-3 and -7 was accompanied by the proteolysis of the DNA repair enzyme, poly (ADP-ribose) polymerase. Induction of apoptosis was also accompanied by the processing of caspase-8, the extent of which varied between patients. Induction of apoptosis and processing of all the caspases was inhibited by the cell permeable caspase inhibitor, benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethyl ketone (Z-VAD.fmk). Our results demonstrate a key role for the activation and processing of caspases in the execution phase of apoptosis in CLL cells. Apoptosis of CLL cells resulted in the selective activation of some but not all caspases. Our results suggest that the dysregulation of apoptosis observed in CLL may be due to the signalling leading to the activation of caspases rather than a deletion of pro-caspases. High levels of caspase-8 in CLL cells in conjunction with low levels of CD95 receptor may offer new therapeutic opportunities for the treatment of CLL. [ABSTRACT FROM AUTHOR]
- Published
- 1998
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- View/download PDF
31. Upregulation of TRAIL-R2 is not involved in HDACi mediated sensitization to TRAIL-induced apoptosis.
- Author
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Inoue, S, Twiddy, D, Dyer, M J S, and Cohen, G M
- Subjects
- *
LETTERS to the editor , *CELL death - Abstract
A letter to the editor is presented in response to the article "Upregulation of TRAIL-R2 is not involved in HDACi mediated sensitization to TRAIL-induced apoptosis," published online in the May 26, 2006 issue.
- Published
- 2006
- Full Text
- View/download PDF
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