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Macrophage-mediated clearance of cells undergoing caspase-3-independent death.
- Source :
-
Cell Death & Differentiation . Mar2003, Vol. 10 Issue 3, p302. 11p. - Publication Year :
- 2003
-
Abstract
- Little is known of the functions of caspases in mediating the surface changes required for phagocytosis of dying cells. Here we investigate the role played by the effector caspase, caspase-3 in this process using the caspase-3-defective MCF-7 breast carcinoma line and derived caspase-3-expressing transfectants. Our results indicate that, while certain typical features of apoptosis induced by etoposide - namely classical morphological changes and the ability to degrade DNA into oligonucleosomal fragments - are caspase-3-dependent, loss of cell adhesion to plastic and the capacity to interact with, and to be phagocytosed by, human monocyte-derived macrophages - both by CD14-dependent and CD14-independent mechanisms - do not require caspase-3. Furthermore, both etoposide-induced caspase-3-positive and -negative MCF-7 cells suppressed proinflammatory cytokine release by macrophages. These results demonstrate directly that cell surface changes that are sufficient for anti-inflammatory clearance by human macrophages can be regulated independently of stereotypical features of the apoptosis programme that require caspase-3.Cell Death and Differentiation (2003) 10, 302-312. doi:10.1038/sj.cdd.4401170 [ABSTRACT FROM AUTHOR]
- Subjects :
- *PHAGOCYTOSIS
*CELL death
*CELL membranes
*MACROPHAGES
Subjects
Details
- Language :
- English
- ISSN :
- 13509047
- Volume :
- 10
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Cell Death & Differentiation
- Publication Type :
- Academic Journal
- Accession number :
- 9537114
- Full Text :
- https://doi.org/10.1038/sj.cdd.4401170