Search

Objective：To identity core genes of neuroinflammation mediated by microglia in sepsis ⁃ associated encephalopathy （SAE）through bioinformatics analysis and validate them through in vitro cellular experiments. Methods：Transcriptomic datasets of peripheral blood from sepsis patients（GSE65682）and in vitro lipopolysaccharide（LPS）⁃ stimulated microglial cell activation model （GSE103156）were obtained from the Gene Expression Omnibus（GEO）database. Weighted gene co ⁃ expression network analysis （WGCNA）was used to screen the modules significantly related to clinical diagnosis of sepsis in the GSE65682 dataset. The intersection between differentially expressed genes（DEG）in LPS⁃treated and untreated microglial cells from the GSE103156 dataset and the WGCNA modules was determined. Functional enrichment analysis of DEG was performed using gene ontology（GO）and Kyoto Encyclopedia of Genes and Genomes（KEGG). A protein⁃protein interaction network was constructed using STRING，and core genes were screened by Cytoscape and Lasso regression analysis. An in vitro cellular activation model of LPS ⁃induced BV2 microglial was established，and gene expression was detected using quantitative real ⁃time PCR（RT ⁃ qPCR). Histone deacetylase 9（HDAC9）was overexpressed in microglia using the lentiviral vector method，and Western blot was employed to detect the inflammation related molecule expression. Results：The WGCNA analysis identified nine modules associated with the clinical diagnosis of sepsis in the GSE65682 dataset，comprising 332 genes. Limma analysis identified 1 272 DEGs in LPS ⁃ stimulated microglial cells from the GSE103156 dataset. Eighteen overlapping genes were obtained，and the Lasso regression analysis further selected four hub genes：G protein ⁃ coupled receptor 183（GPR183), HDAC9，nicotinamide adenine dinucleotide kinase（NADK), and leucine rich repeat containing 25（LRRC25). RT⁃qPCR confirmed downregulation of mRNA expression of Gpr183 and Hdac9 genes and upregulation of Lrrc25 expression in the inflammatory activation model of LPS ⁃ stimulated microglial cell，with no significant change in Nadk expression. Western blot showed that overexpression of HDAC9 promoted the expression of pro⁃inflammatory cytokines interleukin（IL）⁃ 1β and inducible nitric oxide synthase（iNOS）in LPS ⁃ induced microglial cells and enhanced JAK1 ⁃ STAT3 phosphorylation. Conclusion：This study identitfies four key genes mediating neuroinflammation in SAE through bioinformatics analysis and preliminarily demonstrates that HDAC9 has pro ⁃ inflammatory activity in microglia，providing new insights and data for further mechanistic research on SAE. [ABSTRACT FROM AUTHOR]

Objective To understand the community composition of tick - borne pathogens carried by Ixodes persulcatus and the co - infection of Candidatus Rickettsia tarasevichiae (CRT) and Beiji nairovirus (BJNV) in ticks in the Arshan area of the Inner Mongolia Autonomous Region. Methods Free ticks were collected from BaiLang Town, Arshan City, China, and identified by morphological and molecular biology. Total RNA was extracted from tick samples containing unknown pathogens, and cDNA was synthesized and analyzed by metagenomic next generation sequencing (mNGS). The emerging disease - causing pathogens with high abundance at the level of viral and bacterial genera were selected for the analysis of infections and co - infections. The infection of CRT and BJNV in ticks was detected by reverse transcription - polymerase chain reaction (RT - PCR). Sequencing results were analyzed for homology and phylogenetic relationships. Results A total of 617 ticks were collected in this study, all of which were I. persulcatus. High - throughput sequencing results showed that the bacterial composition at the genus level, Rickettsia had the highest abundance of about 77%, and at the species level, Rickettsia canadensis had the highest abundance of about 25%, CRT 1%. Viral composition was highest at the genus level for Orthonairovirus at approximately 60% abundance and at the species level for BJNV at approximately 45 % abundance. CRT and BJNV infections were detected in the samples with a pool positivity rate (PPR) of 96. 15% and 88. 46%, respectively, and there was a compound infection, with a minimum infection rate (MIR) of 3. 72% for the compound infection. Conclusion This study reveals the composition of active species in the microbial community of free - ranging I. persulcatus in Arshan area through mNGS, and find for the first time that CRT andBJNV co - infections exist in I. persulcatus in this area. Therefore, the potential public health risk of CRT and BJNV in this region should be assessed to prevent the occurrence of zoonotic diseases, and the outbreak surveillance of CRT and BJNV - induced diseases should be strengthened. [ABSTRACT FROM AUTHOR]

Objective To study the timeliness of three types of influenza monitoring data in Zhanjiang city, and to provide a basis for future influenza monitoring and warning in Zhanjiang city. Methods For the influenza surveillance data from the 1st week of 2017 to the 52nd week of 2022 in Zhanjiang City, including the number of influenza 一 reported cases, the percentage of influenza - like illness cases (ILI%), and the virus positivity rate, a time series graph was plotted to compare peak times pairwise. Time cross 一 correlation analysis was conducted using the CCF function, and an early abnormal reporting system (EARS) analysis was employed. Results The peak positions of the number of influenza 一 reported cases and ILI% were highly consistent. The main peaks of the three types of influenza monitoring data appeared at similar times each year, but the correlation between small peaks was not clear enough. The correlation coefficient between the current week's number of influenza 一 reported cases and ILI% was the highest (r =0.713, P <0. 01); the correlation coefficient between the number of influenza 一 reported cases reported one week later and the positive rate of the virus was the highest (r =0. 408, P < 0. 01). ILI% had the best ability to warn of Zhanjiang city' s autumn influenza peak. The positive rate of the virus usually appeared as an early warning signal in winter and had a good predictive effect on the arrival of the winter influenza peak in Zhanjiang city. Conclusion The overall trend reflected by the three types of influenza monitoring data in Zhanjiang is consistent, but there are certain differences in timeliness. Therefore, it is necessary to combine multiple monitoring methods to complement each other to achieve effective prevention and control of influenza epidemics. [ABSTRACT FROM AUTHOR]

With the continuous increase of air traffic volume，the vulnerability of the air route network topology has become increasingly prominent， and its robustness optimization has become increasingly important. This paper proposes a method called prefrential configuration node‑protecting cycle（PCNC）that uses complex network theory to analyze the topology structure of air route network. Based on degree fitness technology，it detects key nodes with large degree values of itself and neighbor nodes，increases links， configures node protection rings，and optimizes air route network topology robustness. Taking the air route network in the mainland of China as an example，1 017 waypoints and 1 568 segments were selected；101 key nodes were detected；and 56 new edges were configured. Combined with random attacks and attempted attacks based on node degrees，robustness of the air route network topology for both attack modes is significantly enhanced after optimized，and the robustness of the ARN topology under deliberate attacks is better. [ABSTRACT FROM AUTHOR]

乳腺癌是女性最常见的恶性肿瘤之一，其发病率在全球范围内呈明显上升趋势。随着药理学和分子生 物学研究的进一步深入，靶向药物的研究与应用取得较大突破，其具有直接性和毒性作用小等特点，可直接作用于 病变部位，减少对正常组织和细胞的损害，目前以磷脂酰肌醇3激酶/蛋白激酶B/哺乳动物雷帕霉素、细胞周期蛋 白依赖性激酶(CDＫ)、ADP-核糖多聚合酶(PARP)、人类表皮生长因子受体2(HER2)等为靶点的乳腺癌药物研究 及应用较为广泛。 [ABSTRACT FROM AUTHOR]

BACKGROUND: The hydrochemical characteristics of geothermal water include various information such as geological, structural, fracture, alteration, and environmental changes during its formation. It is particularly important to analyse various components in geothermal water accurately. Tibet is rich in geothermal resources, and some geothermal water contains colloidal particles. It is of great significance to study the morphology, composition and structure of colloidal particles for understanding the relationship between the material sources of geothermal fluids and colloidal particles. The generation and precipitation of colloidal particles can affect the turbidity, conductivity, and partial cation content of geothermal water. It is not easy to obtain naturally formed colloidal particles in geothermal water, so there are few reports on the analysis of colloidal particles in geothermal water. OBJECTIVES: To analyze colloidal particles in geothermal water accurately by multiple methods, and understand the relationship between colloidal particles and opals, and the impact of colloidal particles on the analysis of other elements in geothermal water. METHODS: By comparing the changes in the main components of the water samples before and after filtration, the composition of colloidal particles was inferred. The composition and structure of colloidal particles in the filtrate were characterized by scanning electron microscopy (SEM) and other instruments. The particle size of colloidal particles in geothermal water was measured by laser particle size analyzer, the morphology of colloidal particles was characterized by transmission electron microscope (TEM) and SEM, the characteristic spectrum peak of colloidal particles was measured by Fourier transform infrared spectroscopy (FTIR), the main composition of colloidal particles was analyzed by energy dispersive spectrometer (EDS), and the content of silicon in geothermal water was determined by inductively coupled plasma-optical emission spectrometry (ICP-OES) and ultraviolet-visible spectrophotometry (UV-Vis). RESULTS: The colloidal particles in geothermal water are colloidal silica particles. High levels of silica in geothermal water are present in the form of soluble silicic acid and colloidal particles. The average particle size of the colloidal particles is 80.83nm, which is related to the formation of cesium silica and the enrichment of light rare earth elements. The colloidal particle can be analyzed by ICP-OES method, but cannot show color with ammonium molybdate, resulting in a significant difference in the results of using UV-Vis and ICP-OES to determine silicon in this type of water. ICP-OES is a more suitable detection method for geothermal water with high silicon dioxide content. Silicon dioxide can be directly measured whether it exists in the form of metasilicic acid or colloidal particles. CONCLUSIONS: The research shows that the presence of colloidal particles increases the turbidity, but not the conductivity, of geothermal water. The results show that the high content of SiO2 in geothermal water co-exists in the form of metasilicate and colloidal particles, which can be analyzed by ICP-OES but cannot show color with ammonium molybdate. The content of SiO2 colloidal particles in geothermal water can be obtained by calculating the difference between SiO2 measured by ICP-OES and H2SiO3 measured by UV-Vis. SiO2 colloidal particles are relatively stable and not easy to precipitate. SiO2 colloidal particles will adsorb metal ions in geothermal water during the filtration process. [ABSTRACT FROM AUTHOR]

Objective To explore the specific effect of LL-37 on the destruction of the transmembrane barrier function of bladder urothelial cells and develop a cellular experimental model suitable for studying interstitial cystitis （IC） in vitro. Methods Human urothelial immortalized cells SV-HUC-1 were treated with LL-37 at different concentrations. The transepithelial electrical resistance （TEER） was measured by a transepithelial resistor, and the cell proliferative activity was detected by CCK-8 kit. Cell cycle and calcium ion concentration were detected by flow cytometry. The expression levels of heparan sulfate, a key component of glycosaminoglycan （GAGs）, were detected by RT-qPCR and WB. Results In vitro measurement showed that 100 and 200 μg/mL of LL-37 significantly inhibited the TEER and destroyed the transmembrane barrier function of SV-HUC-1. The CCK-8 test and PI staining flow cytometry results showed that LL-37 above 100 μg/mL could significantly reduce the proliferation activity of SV-HUC-1 and increase the proportion of SV-HUC-1 in the G0/G1 phase （P < 0.05）, suggesting that cell proliferation was significantly inhibited. Further flow cytometry analysis showed that the calcium ion concentration in SV-HUC-1treated with LL-37 was significantly increased （P < 0.05）, and the increase was related to LL-37 concentration. RT-qPCR and WB assays confirmed significant up-regulation of heparan sulfate, a key GAGs component, in the LL-37-treated SV-HUC-1 （P < 0.05）. Conclusion The cathelicidin LL-37 can inhibit the expression of GAGs and destroy the cell proliferation and transmembrane barrier function of SV-HUC-1. [ABSTRACT FROM AUTHOR]

A magnetic sensitive penetrating layer counting method is proposed to solve the problem of signal sticking when the penetration fuze is penetrated at high speed. Magnetic steel and magnetic sensors are installed in the penetration fuze. When the warhead penetrates into the finite thick reinforced concrete target, the magnetic field strength in the fuze changes significantly with the change of the relative position of the target and the warhead, and can be used as a response signals of penetrated layers. The response signal of the penetrated layer is detected to count the layers by using the magnetic sensor. According to the proposed method, the penetrating warhead and reinforced concrete models were established by finite element method, and the penetration process was simulated. A magnetic sensitive simulation fuze was designed and fabricated. The reinforced concrete target was simulated by steel mesh, and the static semi-physical test was carried out to verify the feasibility of the magnetic susceptibility layer method. The proposed method has the advantages of being less susceptible to speed and vibration, and can realize the accurate metering when the penetrating warhead with large aspect ratio penetrates the multi-layer concrete target at high speed. [ABSTRACT FROM AUTHOR]