内蒙古阿尔山地区全沟硬蜱新塔拉塞维奇立 克次体及北极内罗病毒复合感染情况调查

Objective To understand the community composition of tick - borne pathogens carried by Ixodes persulcatus and the co - infection of Candidatus Rickettsia tarasevichiae (CRT) and Beiji nairovirus (BJNV) in ticks in the Arshan area of the Inner Mongolia Autonomous Region. Methods Free ticks were collected from BaiLang Town, Arshan City, China, and identified by morphological and molecular biology. Total RNA was extracted from tick samples containing unknown pathogens, and cDNA was synthesized and analyzed by metagenomic next generation sequencing (mNGS). The emerging disease - causing pathogens with high abundance at the level of viral and bacterial genera were selected for the analysis of infections and co - infections. The infection of CRT and BJNV in ticks was detected by reverse transcription - polymerase chain reaction (RT - PCR). Sequencing results were analyzed for homology and phylogenetic relationships. Results A total of 617 ticks were collected in this study, all of which were I. persulcatus. High - throughput sequencing results showed that the bacterial composition at the genus level, Rickettsia had the highest abundance of about 77%, and at the species level, Rickettsia canadensis had the highest abundance of about 25%, CRT 1%. Viral composition was highest at the genus level for Orthonairovirus at approximately 60% abundance and at the species level for BJNV at approximately 45 % abundance. CRT and BJNV infections were detected in the samples with a pool positivity rate (PPR) of 96. 15% and 88. 46%, respectively, and there was a compound infection, with a minimum infection rate (MIR) of 3. 72% for the compound infection. Conclusion This study reveals the composition of active species in the microbial community of free - ranging I. persulcatus in Arshan area through mNGS, and find for the first time that CRT andBJNV co - infections exist in I. persulcatus in this area. Therefore, the potential public health risk of CRT and BJNV in this region should be assessed to prevent the occurrence of zoonotic diseases, and the outbreak surveillance of CRT and BJNV - induced diseases should be strengthened. [ABSTRACT FROM AUTHOR]