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Hericium erinaceus is a valuable medicinal and edible macrofungus, which has anti-inflammatory, anti-tumor, and immunity-increasing effects. Gas chromatography-mass spectrometer (GC-MS) metabolomics was used to investigate the anti-fatigue effect and mechanism interpretation of Hericium erinaceus polysaccharide on rat negative gravity exhaustion swimming model. The rats were randomly divided into 6 groups (blank group, model group, 3 experimental groups with low, medium and high doses of Hericium erinaceus polysaccharides, and positive control group), with 6 rats in each group. After continuous gastric gavage for 21 days, GC-MS technology was used to analyze metabolites in the serum of rats in each group, and the differential metabolite information was obtained by multivariate statistical analysis, and the anti-fatigue effect of metabolic pathway was constructed to explain. The results show that the swimming time of rats in the high-dose group of Hericium erinaceus polysaccharide is significantly longer than that in the model group. The metabolic profile of rats in the dosing group is close to that of the blank group, showing certain anti-fatigue effects. The differential metabolites in serum 24 are identified as related to the negative gravity exhaustion swimming model, involving three major metabolic pathways: linoleic acid metabolism, glycerol metabolism, phenylalanine, tyrosine and tryptophan biosynthesis. It is hypothesized that Hericium erinaceus polysaccharides may exert anti-fatigue effects on negative gravity-exhausted swimming model rats by regulating lipid metabolism. [ABSTRACT FROM AUTHOR]

Yupingfeng Powder, a classical prescription in ancient times, is commonly used for the treatment of chronic cough and chronic nephritis in children. It has anti-inflammatory, antioxidant and anti-fatigue effects. Gas chromatography-mass spectrometry (GC-MS) metabolomics was used to investigate the antifatigue effects and mechanistic explanation of Yupingfeng powder on the weightloaded exhaustion swimming model of mice. The mice were randomly divided into 5 groups ( normal group, model group, and low-, medium-, and high doses experimental groups of Yupingfeng powder),with six mice in each group. After 14 days of intragastric administration, the serum of mice was analyzed with GC-MS technique to obtain the differential metabolite information,and multivariate statistical analysis was performed to acquire differential metabolite information to construct metabolic pathways to explain the cause of antifatigue. The time span of weight-loaded exhaustion swimming in the Yupingfeng powder group is significantly prolonged than that in the the model group, and the metabolic profiles of mice in the dosing group are found to be closer to those of the normal group, indicating that Yupingfeng powder has certain antifatigue ability. Eighteen differential metabolites in serum are identified to be associated with the weight-bearing fatigue swimming model, three main metabolic pathways involved to be the linoleic acid metabolism, the glycerol metabolism and the phosphoinositide metabolism, the antifatigue mechanism of Yupingfeng powder may be achieved by regulating lipid metabolism. [ABSTRACT FROM AUTHOR]

To investigate the nephrotoxic effect and related pathway evaluation of Yiguan Decoction on rats using gas chromatography-mass spectrometry (GC-MS) metabolomics method. A blank group, a model group, and a consistently high, medium, and low dose administration group were set up, with rats continuously gavaged for 21 days. GC-MS technology was used to analyze the serum of rats in each group and obtain differential metabolite information. Principal component analysis (PCA) was used to classify the metabolites in the blank group and model group, and the differential metabolites and changes in content in the serum of rats with Yiguan Decoction intervention were searched and analyzed. The results show that pathological sections of the kidneys of rats in the Yiguan Decoction high, medium, and low dose groups show varying degrees of damage. The metabolic profile of the high-dose group rats approach that of the model group. 16 differential metabolites in serum were identified. The three main metabolic pathways involved in the Yiguan Decoction are: biosynthesis of phenylalanine, tyrosine, and tryptophan, sulfur metabolism, and glyceride metabolism. [ABSTRACT FROM AUTHOR]

To explore the effects of radiation injury on metabolic pathways by detecting the volatile organic compounds (VOCs) in exhaled breath of rats with radiation-induced lung injury based on gas chromatography-mass spectrometry(GC-MS), and to establish a non-invasive, convenient and rapid method for the diagnosis of radiation injury. In response to the characteristics of the radiation injury model, a rat radiation lung injury model was established using gradient dose irradiation with a cobalt source, and the model rats were randomly divided into four groups, one blank group (0 Gy) and one irradiation group each of 2, 4 and 6 Gy. The exhaled breath were collected at 1, 3, 5, 7 days after irradiation and analyzed by GC-MS. The samples were analyzed by principal component analysis ( PCA) and orthogonal partial least squares-discriminant analysis( OPLS-DA) to find the differential metabolites of relevant VIP > 1, and further explore the potential markers of radiation-induced lung injury. The results show that there are significant differences in blood phase indexes, HE stained sections of lung tissue and VOCs in exhaled breath between the blank and irradiation groups. 25 kinds of metabolites are initially screened, among which 5 kinds could be used as combinative potential biomarkers. The construction of this discriminant model provides a theoretical basis for the respiratory histology of radiation injury and an experimental reference for the establishment of a diagnostic method for the non-invasive detection of radiation injury. [ABSTRACT FROM AUTHOR]

The mechanism of Baoyuan Decoction on mice with blood deficiency syndrome was investigated by metabonomics based on gas chromatography-mass spectrometer（GC-MS）. Intraperitoneally injected Kunming mice with cyclophosphamide（CTX） to establish a mouse model of blood deficiency syndrome characterized by decreased leukocyte count. Normal control group, model group, positive control group and Baoyuan Decoction low, medium and high dose groups were established for 14 days. Gas chromatography-mass spectrometry was used to analyze the serum of mice in each experimental group. The metabolites of normal control group and model group were classified by principal component analysis（PCA）, in order to find the the content changes of differential metabolites after Baoyuan Decoction acted on blood deficiency syndrome model mice. In the mean time, the blood biochemical indexes were detected and the organ index of mice were calculated in each group. The results show that Baoyuan Decoction could significantly increase the number of leukocytes, repair the damage of thymus and spleen in blood deficiency syndrome mice. The metabolic profile of mice in the therapeutic group approached the blank control group. 10 different metabolites were found in serum related to the immunosuppressive blood deficiency syndrome caused by CTX, mainly involving three metabolic pathways such as linoleic acid metabolism, therefore speculated that the therapeutic effect of Baoyuan Decoction on blood deficiency syndrome may be achieved by regulating fatty acids, [ABSTRACT FROM AUTHOR]

Drug induced renal injury is a public health issue that has received much attention. Currently, there is a lack of effective technical means for the clinical diagnosis of early renal injury, leading to the failure of timely treatment of the disease, ultimately endangering the life safety of patients. N-acetyl-β-D-glucosaminidase(NAG) is a new type of biomarker for renal injury, and the activity of NAG in urine can significantly change during early renal injury. Therefore, the development of accurate, batch, and low-cost detection techniques for NAG is of great significance for the early diagnosis and treatment of kidney diseases. Therefore, scholars have done a lot of research work in recent decades. The research progress of NAG detection methods was summarized, the advantages and disadvantages of various methods was analyzed, and finally the development direction of NAG detection technology was discussed. [ABSTRACT FROM AUTHOR]