基于气相色谱￣质谱联用代谢组学技术研究 保元汤对血虚证小鼠的影响.

The mechanism of Baoyuan Decoction on mice with blood deficiency syndrome was investigated by metabonomics based on gas chromatography-mass spectrometer（GC-MS）. Intraperitoneally injected Kunming mice with cyclophosphamide（CTX） to establish a mouse model of blood deficiency syndrome characterized by decreased leukocyte count. Normal control group, model group, positive control group and Baoyuan Decoction low, medium and high dose groups were established for 14 days. Gas chromatography-mass spectrometry was used to analyze the serum of mice in each experimental group. The metabolites of normal control group and model group were classified by principal component analysis（PCA）, in order to find the the content changes of differential metabolites after Baoyuan Decoction acted on blood deficiency syndrome model mice. In the mean time, the blood biochemical indexes were detected and the organ index of mice were calculated in each group. The results show that Baoyuan Decoction could significantly increase the number of leukocytes, repair the damage of thymus and spleen in blood deficiency syndrome mice. The metabolic profile of mice in the therapeutic group approached the blank control group. 10 different metabolites were found in serum related to the immunosuppressive blood deficiency syndrome caused by CTX, mainly involving three metabolic pathways such as linoleic acid metabolism, therefore speculated that the therapeutic effect of Baoyuan Decoction on blood deficiency syndrome may be achieved by regulating fatty acids, [ABSTRACT FROM AUTHOR]