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The value of the slope ratio of the foundation trench for offshore immersed tube tunnels has a significant impact onthe engineering economy and construction risks. Dredging of waterways near the foundation trench may lead to abnormalsiltation of the foundation trench, affecting the stability of the foundation trench slope and thus affecting construction progress.By conducting in -situ trial excavation tests in the tunnel site area and numerical simulation analysis of adjacent waterwaydredging, the stability and siltation of the foundation trench slope were analyzed. This provides technical support for the designand construction of the foundation trench scheme for the Hongkong-Zhuhai-Macao Bridge immersed tube tunnel. The researchresults show that: 1) Observations of the flood season impact cycle shows that the foundation trench slopes with slope ratios of1:5, 1:6, 1:8, and 1:10 have no structural changes, and are overall stable. 2) The bottom area of the trial excavation trenchshows a continuous siltation state, while the outer area of the slope shows an alternating phenomenon of siltation and erosion,but the overall change amplitude is not significant. 3) The closer the waterway dredging is to the position of the foundationtrench, the greater the impact on the siltation of the foundation trench. The location where the foundation trench siltation isthickest appears at the intersection of the foundation trench and the waterway [ABSTRACT FROM AUTHOR]

Objective: To investigate the predictive value of endotoxin (Endotoxin), procalcitonin (PCT) combined with neutrophil to lymphocyte ratio (NLR) in predicting urinary sepsis in patients after percutaneous nephrolithotomy (PCNL). Methods: 750 patients who underwent PCNL in the Department of Urology Surgery, The Second Affiliated Hospital of Xi’an Medical College and The First Affiliated Hospital of Air Force Military Medical University from May 2020 to May 2023 were selected as study subjects. Patients were divided into urinary sepsis group (n=45) and non sepsis group (n=705) according to the occurrence of urinary sepsis. The levels of serum Endotoxin, PCT, neutrophils and lymphocytes before PCNL were detected, and NLR was calculated. Serum Endotoxin, PCT levels and NLR were compared in two groups. The factors affecting the risk of urinary sepsis in patients after PCNL were analyzed by multivariate Logistic regression model. The clinical efficacy of serum Endotoxin, PCT combine with NLR in predicting urinary sepsis in patients after PCNL were analyzed by drawn receiver operating characteristic (ROC) curve. Results: Compared with non sepsis group, the serum Endotoxin, PCT and NLR in urinary sepsis group were higher (P＜0.05). Multivariate Logistic regression model analysis showed that, elevated serum Endotoxin, elevated PCT, elevated NLR, positive urine white blood cells, preoperative fever and staghorn calculi were independent risk factors for urinary sepsis in patients after PCNL (P＜0.05). The results of ROC curve analysis showed that, the area under the curve (AUC) of serum Endotoxin, PCT combine with NLR in predicting urinary sepsis in patients after PCNL was 0.913, which was higher than that of the above indicators alone. Conclusion: The increase of serum Endotoxin, PCT and NLR before PCNL may be relate to the occurrence of urinary sepsis in patients after PCNL. Elevate serum Endotoxin and PCT levels, elevate NLR, preoperative fever, positive urine white blood cells and staghorn calculi are risk factors for urinary sepsis in patients after PCNL. Serum Endotoxin, PCT combine with NLR detection has a high predictive value for urinary sepsis in patients after PCNL. [ABSTRACT FROM AUTHOR]

Objective To compare the computed tomography (CT) signs of Corona Virus Disease 2019 (COVID-19) and pneumocystis jirovecii pneumonia (PJP),and improve the level of differential diagnosis.Methods The first CT data of 32 COVID-19 patients admitted to Bao Ding No.1 Central Hospital and Bao Ding infectious Diseases Hospital of Hebei Province and 10 PJP patients admitted to Bao Ding No.1 Central Hospital of Hebei Province from December 2019 to March 2020 were collected, and their CT signs were compared.Results The COVID-19 patients were mainly characterized by multiple lung lesions in subpleural area, and their probability of pleural parallel sign and vascular thickening sign were significantly higher than those of the PJP patients, while the probability of pulmonary balloon sign, lunar arch sign and map sign were significantly lower than those of the PJP patients, with statistical significance (P<0.05) .Conclusion COVID-19 and PJP not only share common CT features but also have typical imaging features and chest CT plays an important role in the differential diagnosis of COVID-19 and PJP. [ABSTRACT FROM AUTHOR]

Carbon monoxide (CO) is the main component of vehicle exhaust in highway tunnels, which is one of the important parameters for the design of ventilation in road tunnels. It is very necessary to predict the CO concentration accurately and quickly to provide a reference for tunnel ventilation system control and mitigating the concentration of CO timely and to ensure the health and safety of the people in the tunnel. At the same time, it is beneficial to the green and energy-saving operation of the tunnel. Based on the field monitoring data of the road tunnel, random forest (RF) model of CO concentration prediction was established with the four influencing factors, i. e., monitor position, traffic, vehicle speed, wind speed. The model was employed to predict the concentration of CO in a 3 300-long road tunnel and the predictive values were compared with the real values. The results show that the CO concentration prediction model based on Random Forest has good prediction accuracy, which can predict the concentration of CO accurately. The RMSE and R 2 of the testing dataset is 0. 497 4 and 0. 943 7, respectively, which is significantly better than those of the linear regression model and support vector machine (SVM) model. The prediction model can be extended to predict the CO concentration of another tunnel. The RMSE and R 2 of the validation dataset is 0. 909 5 and 0. 729 5, respectively. That is, the CO concentration in the tunnel can be predicted under the condition of known monitor position, traffic, vehicle speed, wind speed. The model provides a reference for tunnel ventilation system control or safety warning. The position of monitor point has the greatest influence on the CO concentration in the tunnel, and the CO gas concentration is the highest at the exit of the tunnel. With the increase of wind speed, the concentration of CO gas in the tunnel gradually decreases. [ABSTRACT FROM AUTHOR]

The thermal environment of maneuvering rudder shaft is a local problem to be considered in the design of thermal protection system of hypersonic missile. The flow structure in rudder shaft gap is complex， and under typical flight conditions， the peak of the local thermal environment of the rudder shaft is more serious than that of in large area. Aiming at the problem of thermal environment of hypersonic maneuvering warhead shaft， combined with numerical calculation and wind tunnel test， the variation of thermal environment of rudder shaft with attack angle， rudder deflection angle and Mach number is studied. Then， a four-parameter interpolation fitting method suitable for predicting the peak heat flow of rudder shaft is established. The results show that， for the rudder like letter“ + ”，the thermal environment of horizontal rudder shaft is the most severe； under the condition of small attack angle， the dimensionless heat flow of the rudder shaft increases gradually with the increase of the rudder deflection angle and Mach number， but under the condition of large attack angle， the influence of Mach number and rudder deflection on the dimensionless heat flow of the rudder shaft is small. The four-parameter fitting method established in this paper can be used to rapidly evaluate the peak thermal environment of the rudder shaft along the trajectory. [ABSTRACT FROM AUTHOR]

目的优化新生儿脸表吸入综合征(MASS麵通气的被机方案,政定报机标准并予经鼻间网歇正压通气(nlPPV) 序貫通气过式,客优化方案的安全和有效性,方法选取2017年1月至2019年12月常新生儿童症监护病房教治的常频 机械通气 MAS息儿57例,隨机分为优化组(31例)和封照組 (26 例),化化姐這当放定磁机标准,对照姐达家規搬机标准后 抓机, 2组息儿振机后均予nPV 过波,比较2组惠几板机成功平和并发症发生率,结果优化組息儿常频通气时间为(46,74土 18.86)h,时照组常頻通气时间为(72, 23土24.32)h,优化组有创通气时间 过于对照姐,异有统计学意义(P<0.05);2加息 儿振机成功率、总用氣时阿、天,创通气时间,以及机城通气后可吸机相关性 肺炎、气品发生奉比较,差异均无统计学意义(P> 0.05),结论 对常頻通气的 MAS 患儿可就定椒机标准并予 nlPPV序貫通气过波,安全有政,值符临床的广用. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to select good mulberry varieties suitable for local promotion. [ Method] Four varieties of white mulberry, four varieties of black mulberry, two varieties of pink mulberry and two varieties of purple mulberry in southern and eastern Xinjiang were selected for grafting in Turpan, fruit 88 a sample for measurement, three economic traits, eight nutritional components and taste indexes of fruits were determined and analyzed, Spss Software was used to analyze their difference and correlation, and fuzzy membership function was used for the quality analysis. [ Result] There were extremely significant( P < 0. 01) or significant( P < 0. 05) in economic characters and nutrient components of 12 mulberry varieties; According to the ranking of mean value of membership function, four varieties with good economic characters were selected as follows: Baisang 2 > Heisang 2 > Baisang 3 > Baisang 4, Four varieties with good nutritional components were Baisang 4 > Heisang 1 > Baisang 3 > Fenpang 1. There was no correlation between economic traits and nutritional components. [ Conclusion] Based on the analysis and evaluation of 12 varieties of mulberry fruits, Baisang No. 3 and Baisang No. 4 could be used as fresh food species; Heisang 1 and Fansang 1 could be dried for medicinal use. By breeding methods of asexual and sexual reproduction of white mulberry No. 2, black mulberry No. 2, black mulberry No. 1 and pink mulberry No. 1, the good varieties would be screened out to promote the cultural development of mulberry industry in Turpan. [ABSTRACT FROM AUTHOR]

Using 3 parameters to describe finite rotations will inevitably have the singularity problem, which leads to numerical difficulties in solving the rotational parameters from the integration of the angular velocity. Based on systematical studies of the singularity of the rotation vector, a new numerical integration method, which can overcome singular points of the rotation vector, was proposed. With the property that the 2 rotation vectors with the same direction but different norms correspond to the same finite rotation, the rotation vector near the singular point was switched to its corresponding one far away from the singular point and in the numerical stability region, during the numerical integration. This method can avoid the difficulties in the numerical integration caused by the singularity of rotation vectors for the angular velocity vectors. Numerical examples show that the proposed method is simple, stable and effective. [ABSTRACT FROM AUTHOR]

Based on the comparison and analysis of the foundation bedding of undersea immersed tunnel for Hongkong- Zhuhai-Macao Bridge, we proposed the gravel bedding as the transition layer between the tunnel structure and foundation. By comparing with the foreign similar engineering design, we designed the geometric parameters about the transverse, longitudinal and vertical sides based on action mechanism of gravel layer with ridge and groove, and determined the physical and mechanical indexes by the physical model test. Practical testing show that gravel bedding can meet the design requirements, reasonable construction process connection, which is a kind of underwater structure with the features of detectable, reachable, repairable, it is valuable to popularize and use in similar projects. [ABSTRACT FROM AUTHOR]

The effects of nutrient addition in substrate before sowing on growth and quality of seedling were studied with cucumber ‘ Zhongnong No. 203 , 3'1: 1 (v/v)peat, vermicnlite, and perlite were used as substrate, emergence rate, available N, P, and K content in substrate and morphological index of cucumber plug seedlings were determined. Nutrient content of substrate in root zone increased with the elevation of nutrient addition in substrate, while prophase emergence rate and vigor index of cucumber seedling increased at first and decreased subsequently. A better addition of nutrient in substrate before sowing was 500N-10GP-500K, prophase emergence rate and vigor index were higher than other treatments by 3.35 % -20.13 % , 9.06 % -45.17 %. [ABSTRACT FROM AUTHOR]

The traditional method of determining gold in antimony ores commonly includes sample digestion by aqua regia, preconcentration of gold by activated carbon, and measurement by hydroquinone volumetric technique. The traditional method suffers from the high contents of antimony, sulfur, arsenic, and mercury. Sulfur, arsenic, mercury, and carbon can be removed by stepwise heat, but antimony cannot be removed. In this study, tartaric acid is added to eliminate the interference of antimony. Adding tartaric acid to aqua regia during sample digestion can prevent proteolysis of antimony compounds as the acidity or temperature decreases. This step can ensure that the antimony quantity is less than 0.3 mg after the ashing of activated carbon that has adsorbed gold. After being ashed, tartaric acid is again added in aqua regia to dissolve gold, eliminating the interference of antimony on gold during hydroquinone volumetric determination of gold. The proposed method suggests adding tartaric acid at the sample digestion stage is simple and rapid relative to the method of antimony elimination by hydrobromic acid and hydrochloric acid. The relative standard deviation of natural sample analysis is less than 5% and standard-addition recovery is 92.0%-107.0%. [ABSTRACT FROM AUTHOR]

For shield tunneling under complex geological conditions, scientific setting of tunneling parameters is very important to the safety and efficiency of a project. In order to study the variation law of shield tunneling parameters in composite strata, based on the construction of the left-hand line between Shazikou Station and Laoshan Liuzhong Station of Qingdao Metro Line 4, statistical analysis was made of the tunneling parameters in three different geological environments: tunneling below river bed, homogeneous clay stratum and stratum with soft soil on top and hard soil below. The results of the statistical eigenvalues and correlation analysis showed that: 1) the total propulsive force was the lowest as the tunnel section below riverbed was mainly composed of silty clay mixed with coarse sand; 2) the tunneling parameters for tunneling in the homogeneous clay were stable and the average soil pressure was the highest, with the strongest correlation between the total propulsive force and the driving speed; and 3) the variation frequency and amplitude of the tunneling parameters for tunneling in the stratum with soft soil on top and hard soil below were greater and the driving speed was the slowest. [ABSTRACT FROM AUTHOR]

Objective To explore the effect of UBE2I and FCGR1A gene expressions on the incidence of acquired immune deficiency syndrome (AIDS) combined with active pulmonary tuberculosis (APTB), so as to provide basis for disease monitoring. Methods A total of 98 AIDS patients combined with APTB and 84 AIDS patients combined with latent tuberculosis infection (LTBI) were selected from the validated whole genome transcriptome dataset (GSE37250) . The top 30 differentially expressed genes (DEGs) in the two groups of patients were screened. We established the PPI interaction network, transcription factor-differentially expressed gene (TF-DEG), DEG-miRNA, and environmental chemical regulation network of the top 30 DEGs. Receiver operating characteristic (ROC) curves of 11 key DEGs were plotted and Logistic regression analysis was performed. Results There were 6 054 DEGs in the two groups of patients, and UBE2I was an important core node of the PPI interaction network. FCGR1A had the best predictive and indicative ability for AIDS combined with APTB. Univariate Logistic regression showed that high expressions of UBE2I and FCGR1A were risk factors for AIDS combined with APTB (P<0.05) . The regulatory network showed that VEGFB was a key gene in the TF-DEG network, participating in regulation with transcription factors such as SEPT9 and SMAD5. It targeted miRNAs such as hsa-mir-17-5p and hsa-mir-20a-5p, and was affected by environmental chemicals such as valproic acid and copper sulfate. Conclusion VEGFB plays an important role in the pathogenesis of AIDS combined with APTB. The abnormally high expressions of UBE2I and FCGR1A are associated with the disease progression of AIDS combined with APTB. The disease condition can be monitored by detecting the expression level of UBE2I and FCGR1A. [ABSTRACT FROM AUTHOR]

Objective·To establish a mouse model with dormant cancer and no obvious metastasis by combining the preimmune strategy with the mVenus-p27K－ cell G0 phase indicator system, the DTR-HSV/TK suicide gene system, and the Luc2-tdTomato tracer system. Methods·The KPC1199 mouse pancreatic cancer cell line was transfected with the mVenus-p27K－ cell G0 phase indicator system, the DTR-HSV/TK suicide gene system, and the Luc2-tdTomato tracer system to construct a stable expression cell line, KPC1199-PDL. After being cultured in the serum-free condition, KPC1199-PDL cells were sorted into mVenus (+) cells and mVenus (-) cells by flow cytometry, and the expression of G0 phase-related genes was verified by real-time fluorescence quantitative PCR (qPCR). Sensitivity of KPC1199-PDL cells to diphtheria toxin (DTX) and ganciclovir (GCV) was evaluated by CCK-8 assay. A transsplenic portal vein-hepatic metastasis model was constructed in wild-type C57BL/6 mice to validate the function of KPC1199-PDL cells in vivo by immunofluorescence technology. The KPC1199-PDL cells were injected subcutaneously into C57BL/6 mice, followed by in situ injection of DTX and GCV to ablate subcutaneous tumors 5 d later, to obtain preimmunized mice. The transsplenic portal vein-hepatic metastasis models were constructed in these mice. Bioluminescence imaging was used to evaluate subcutaneous tumor ablation and hepatic metastasis in the mice, and immunofluorescence assay was used to detect the distribution and dormant state of tumor cells in the livers of preimmunize mice. Results·The three tool systems were stably expressed in KPC1199-PDL cells, and their proliferative ability was not affected. In the serum starving condition, some KPC1199-PDL cells expressed the mVenus protein, indicating entry into the G0 phase; the mVenus (+) cells sorted out by flow cytometry exhibited significantly higher expression of G0 phase-related genes (all P<0.05) and significantly lower expression of the proliferation-related gene compared with mVenus (-) cells (P<0.05). The CCK-8 assay demonstrated high sensitivity of KPC1199-PDL cells to DTX and GCV. In vivo experiments confirmed that KPC1199-PDL cells could be effectively traced through tdTomato protein expression, and could indicate entry into the G0 phase through mVenus protein expression. Following subcutaneous tumor implantation and drug ablation, preimmunized mice were successfully obtained. In the subsequent transsplenic portal vein-hepatic metastasis model, no metastatic signals were observed in the liver by bioluminescence imaging, but single or small clusters of G0 phase tumor cells expressing both mVenus and tdTomato, not expressing the proliferation marker Ki67, were detected in liver tissue sections by immunofluorescence analysis. Conclusions·A recognizable and traceable dormant cancer model is constructed with the combination of the preimmune mouse model of pancreatic cancer, the mVeneus-p27K－ indicator system, the DTR-HSV/TK suicide gene system, and the Luc2-tdTomato tracer system. [ABSTRACT FROM AUTHOR]

On the basis of finite element method combined with viscous-elastic artificial boundary, the seismic wave input was transformed into equivalent node force, and a three-dimensional plane SY wave input method based on wave theory was established. The accuracy of seismic wave input method and the valid of the numerical analysis were proved by the half-space example. Then the mechanical properties of the shear keys of the segment joint in Hong KongZhuhai-Macao Bridge immersed tunnel considering the static and dynamic water effects of the upper sea water were studied based on the established seismic wave input method. The results show that the vertical acceleration is also generated by horizontal earthquake, but the value of the vertical acceleration is small. The compressive stress is the largest stress on the shear key under earthquake, while the tensile stress is relatively small. Because the tensile stress of shear key is mainly concentrated on the locations where the shear key is connected with the main body of the immersed tunnel, tensile cracks are prone to occur. The main failure mode of horizontal shear keys is tensile failure. The setting direction of the shear key in the joint determines the shear distribution at the root of the shear key, and most of the shear forces are resisted by the shear key in the same direction with the displacement. [ABSTRACT FROM AUTHOR]

As interstitial collagen, type Ⅲ collagen (Col Ⅲ) does not express in normal glomeruli. However, in Col Ⅲ nephropathy, a large amount of Col Ⅲ deposit in the mesangial and subendothelial area of the glomeruli. IgA nephropathy with Col Ⅲ deposition was extremely rare. In this article, we reported two cases of such disease. After treating with immunosuppressive agents or traditional Chinese medicine decoction, the renal function of the two patients remained stable and the urinary protein levels reduced significantly. [ABSTRACT FROM AUTHOR]

Objective To discuss the feasibility and practice of online examination for undergraduate pathological anatomy course during the COVID-19 epidemic. Methods We used online examination system of superstar at Fudan University Online Education Center to prepare for the examination，invigilate the examination，review the examination papers and analyze the examination papers before and after the examination. Results Based on the online examination of 151 eight-year undergraduates in clinical medicine，we found that the time consistency can be achieved through the setting of‘limited time entry’ and‘limited time submission’in the system.By adding analysis，induction and comparison questions and reducing memory questions，the overall difficulty coefficient（P=0.81）of the test paper was equivalent to that of the same period of offline test in previous years. Through the rehearsal test setup，teachers and examinees could be familiar with the test process and improve the test equipment. The proportion of students using PC computer platform webpage to answer questions in formal examination was about 2%， higher than that in practice test，and another 4% students were no longer use mobile platform program to answer questions. Conclusion The overall running of the online examination is stable and the score distribution is reasonable. The choice of examination platform should be applicable to all examinees.Before the examination，we should fully consider the particularity of the online examination scene，and carry out the simulation test and examination plan design in advance.The test paper formation and question setting of the online examination should be adjusted appropriately. [ABSTRACT FROM AUTHOR]

Objective: To nvestigate he effect of mindfulness herapy n patients with postpartum depression. Methods: PubMed, Cochrane Library, EMbase, Web of Science, CNKI and Wanfang databases were searched for relevant studies on mindfulness therapy for postpartum depression patients, included in randomized con-rolled rials. Two researchers extracted data based on nclusion/exclusion criteria and according o Cochrane Handbooks. 1. 0 manual performs literature quality evaluation. Data were analyzed by using RevMan5. 3 statistical soft-ware. Results: Six randomized controled rials (RCT) studies and 2 self-controlled studies were ncluded, totaling 404 patients. Meta-analysis showed that mindfulness therapy could effectively alleviate maternal depression and anxiety compared with conventional nursing methods. The evel of depression n postpartum depression patients receiv-ng mindfulness therapy was lower than that in he conventional care group. The difference between he two groups was statistically significant (MD = 3. 01,95% CI: 2. 29 - 3. 72, P = 0. 33). Conclusion: Mindfulness therapy has the effect of improving anxiety and depression n patients with postpartum depression. [ABSTRACT FROM AUTHOR]

Objective·To analyze the expression changes of adhesion G protein-coupled receptor F1 (ADGRF1) in the occurrence and development of pancreatic ductal adenocarcinoma (PDAC), and explore the impact of ADGRF1 on the proliferation of PDAC cells and the potential molecular mechanisms that promote PDAC progression. Methods·The expression of ADGRF1 at mRNA level was analyzed based on the Gene Expression Omnibus (GEO) database and The Cancer Genome Atlas (TCGA) database, respectively. The expression of ADGRF1 in normal pancreatic ductal epithelial cells (hTERT-HPNE) and various PDAC tumor cells was detected by using real-time fluorescence quantitative PCR (qPCR) and Western blotting. Immunohistochemical staining (IHC) was used to detect the differential expression of ADGRF1 in cancer tissues and adjacent tissues of PDAC patients. After knocking down ADGRF1 with small interfering RNA (siRNA) transfection, the changes in the proliferation ability of PDAC AsPC-1 and SW1990 cells were detected through CCK8 assay and plate cloning experiment. Stable overexpression of ADGRF1 was constructed in PDAC Patu8988 cell line, and the proliferation changes induced by overexpression of ADGRF1 were evaluated through CCK8 assay. RNA sequencing (RNA-seq), gene set enrichment analysis (GSEA), and immune infiltration analysis were utilized to predict signaling pathways associated with ADGRF1-mediated promotion of PDAC cancer progression. Results·Analysis of the TCGA database and GEO database revealed higher expression of ADGRF1 mRNA in PDAC tissues compared to normal pancreatic tissues (all P=0.000). qPCR and Western blotting results demonstrated up-regulation of ADGRF1 mRNA and protein levels in various PDAC cells compared to hTERT-HPNE cells (all P<0.05). IHC results confirmed higher ADGRF1 expression in PDAC cancer tissues compared to adjacent tissues. Furthermore, downregulation of ADGRF1 inhibited the proliferation of PDAC AsPC-1 and SW1990 cell lines, while overexpression of ADGRF1 promoted the proliferation of Patu8988 cells (all P<0.05). RNA-seq, GSEA enrichment analysis, and immune infiltration analysis revealed that ADGRF1 expression was related to signaling pathways such as interferon-α (IFN-α), tumor necrosis factor-α (TNF-α), and nuclear factor κB (NF-κB). Conclusion·ADGRF1 is highly expressed in PDAC cells and tissues, and promotes the proliferation of PDAC cells via immune-related signaling pathways. [ABSTRACT FROM AUTHOR]

Objective To investigate the correlation between YAP and podocytes apoptosis in focal segmental glomerulosclerosis (FSGS) and the role of YAP activator on the podocytes apoptosis. Methods The renal biopsy specimens of human FSGS were collected from the Nephrology Research Group of the Department of Pathology, School of Basic Medical Sciences, Fudan University. Adriamycininduced FSGS mice model and Adriamycin-induced podocytes injury model in vitro were generated, and the above in vivo or in vitro model were treated in combination with YAP activator 1-Oleoyl lysophosphatidic acid (LPA) . Immunohistochemistry (IHC) or double immunofluorescence staining, Western blot and Hoechst 33258 staining were used to examine the correlation between podocytes apoptosis and the expression of YAP in podocytes. Podocytes transfected with YAP siRNA were treated with LPA, and then YAP expression and podocytes apoptosis were examined. Results The more apoptotic podocytes, the more nuclear exclusion of YAP in FSGS were observed either in vivo or in vitro. YAP activator LPA improved glomerular morphology (t=17.68, P<0.000 1) and renal function (BUN: t= 4.576, P=0.010 2; UACR: t=2.51, P=0.045 6) of Adriamycin-induced FSGS mice model, reduced the expression of p-YAP (S127), increased YAP nuclear entry and decreased podocyte apoptosis (P<0.001 for both expression of Cleaved Caspase-3 and Hoechst 33258 staining) induced by Adriamycin both in vivo and in vitro. RNA interference of YAP decreased both total and phosphorylated YAP expression, and increased podocytes apoptosis. LPA treatment decreased the expression of p-YAP (S127), promoted YAP nuclear localization and decreased the podocytes apoptosis (P<0.000 1 for both expression of Cleaved Caspase-3 and Hoechst 33258 staining) induced by YAP interference. Conclusion There are decrease in YAP activation, nuclear exclusion of YAP, and increased podocytes apoptosis in FSGS model. LPA can inhibit the phosphorylation of YAP at Ser127, promote its nuclear translocation, thus to protect podocytes from apoptosis to ameliorate the progression of FSGS. [ABSTRACT FROM AUTHOR]

In this study, the infiltration and redistribution lac characteristics of cater in sandy loamsoil cere investigated to provide the basis for the rational and efficient water-saving irrigation of jujube. Based on jujube root distriljution in the sandy loam, ECH&0 soil moistiare sensors and 48-channel automatic data loggers made by Decagon Company cere set to obtain the characteristics valiae of cetting soil in the stage of irrigation and after irrigation. According to these characteristic values, the moist ratio, time of migration and migration rate in horizontal and vertical direction cere compared, understood the changes of soil moisture content and drac cet body distribution range. The result shoced that (i) In the early stage of irrigation, the cater movement distance and velocity of tlie diferent emitter floc rate in the level direction cere higher than that in the vertical direction. (ii) The cetted body redistribution cater cas greater than that of the end of the irrigation cater, the cater content rate of the end of the irrigation cater cas greater than that of the cetted body redistribution cater in the same position. (iii) The cater characteristic values and moist body distributions in sandy loamsoil could provide the basis for the irrigation-saving cater system of jujube. [ABSTRACT FROM AUTHOR]

Objective To observe the effect of the Hps1 and Hps4 subunits of biogenesis of lysosome ⁃ related organelles complex ⁃ 3 (BLOC⁃3) on the mitochondrial localization of Rab32 in liver cancer cells and the role of liver cancer growth. Methods The interaction of Hps1 and Hps4 with Rab32 was analyzed by public databases GenDoma, String and InBio Discover. Human liver cancer cell lines SNU⁃739 and Hep⁃3B were cultured in vitro and transfected with Hps1 and Hps4⁃related siRNAs and plasmids, respectively, by using the liposome transfection method. The effect of Hps1 and Hps4 on the mitochondrial localization of Rab32 was observed by immunofluorescence and Western blot. Cell scratch, cladogenesis, EdU, MTS and Trans well invasion assays were used to detect the changes of liver cancer cell migration, proliferation and invasion after the regulation of Rab32 mitochondrial localization by Hps1 and Hps4. The expression difference of Rab32 protein in liver cancer and normal liver tissues was analyzed by using dataset CPTAC in the public database UALCAN. Results Database analysis showed that both Hps1 and Hps4 could interact with Rab32. Rab32 protein expression was significantly decreased in liver cancer tissues compared with normal liver tissues (P<0.001). After interference with Hps1 or Hps4 or both Hps1 and Hps4 in liver cancer cell line SNU⁃739, the mitochondrial localization of Rab32 were decreased (all P<0.01), mitochondrial Rab32 protein expression were decreased (all P<0.001), and cell proliferation, migration and invasion were enhanced (all P<0.05). After overexpression of Hps1 and Hps4 in liver cancer cell line Hep⁃3B, the mitochondrial localization of Rab32 was increased (P<0.01), the protein expression of mitochondrial Rab32 was increased (P<0.001), and the proliferation, migration and invasion of cells were inhibited (all P<0.01), while the overexpression of Hps1 or Hps4 alone had no significant inhibitory effect (all P>0.05). Conclusions Both Hps1 and Hps4, the subunits of BLOC⁃3, can interact with Rab32 and increase the mitochondrial localization of Rab32, thereby inhibiting the growth of liver cancer cells. [ABSTRACT FROM AUTHOR]

Objective: To compare the clinical efficacy of gemcitabine and epirubicin intravesical chemotherapy in patients with non muscle invasive bladder cancer (NMIBC) after transurethral resection of bladder tumor (TURBT) and analyze the risk factors for recurrence. Methods:120 patients with NMIBC who were received in Lianyungang Hospital Affiliated to Xuzhou Medical University from January 2018 to January 2020 were included and all received TURBT treatment. According to the different ways of chemotherapy, 120 patients with NMIBC were divided into epirubicin group (n=58, epirubicin treatment) and gemcitabine group (n=62, gemcitabine treatment). The changes of immune function and tumor markers in epirubicin group and gemcitabine group were compared, and the incidence of adverse reactions and 1-year recurrence were observed. Multivariate logistic regression was used to analyze the risk factors of recurrence in patients with NMIBC after TURBT. Results: After treatment, the levels of CD3+, CD4+, CD4+ /CD8+ in epirubicin group and gemcitabine group were increased, and the gemcitabine group were higher than those in epirubicin group (P<0.05). CD8+ decreased, and gemcitabine group was lower than epirubicin group (P<0.05). After treatment, carcinoembryonic antigen (CEA), cytokeratin 19 (CK19) and cancer antigen 199 (CA199) in epirubicin group and gemcitabine group decreased, and gemcitabine group was lower than that in epirubicin group (P<0.05). There was no significant difference in the incidence of adverse reactions between epirubicin group and gemcitabine group (P>0.05). There was no significant difference in the 1-year recurrence rate between epirubicin group and gemcitabine group (P>0.05). 28 patients in epirubicin group and gemcitabine group who recurred after one year were included in the recurrence group, and the other 92 patients who did not recur were included in the non recurrence group. Univariate analysis showed that the recurrence of NMIBC patients after TURBT was related to the number of tumors, maximum tumor diameter, tumor differentiation grade and clinical stage (P<0.05). Multivariate logistic regression analysis showed that the number of tumors was multiple, the maximum tumor diameter 逸5 cm, the tumor differentiation grade was poorly differentiated, and the clinical stage was T1 stage, which were the risk factors of recurrence in patients with NMIBC after TURBT (P<0.05). Conclusion: Compared with epirubicin chemotherapy, gemcitabine chemotherapy can better control the progression of tumor diseases and effectively protect the immune function. The safety and recurrence rate of the two chemotherapy drugs are similar. At the same time, the study also observed that recurrence was affected by many factors, including tumor number, maximum tumor diameter, tumor differentiation and grade, and clinical stage. [ABSTRACT FROM AUTHOR]

【Objective】In order to study the effects of different foliar fertilizers on apple light response parameters, the best light response model and foliar fertilizer formula were selected.【Method】The different kind of foliar fertilizer treatments on apple were fitted by rectangular hyperbola model, non-rectangular hyperbola model, modified rectangular hyperbola model and exponential model respectively photosynthesis-light response curve. The best foliar fertilizer was evaluated by PCA. 【Result】As the light intensity increased, the net photosynthetic rate of all treatments showed a ‘slow-fast-slow’ trend, except formula Ⅳ, at the light intensity of 1900 μmol·m-2·s-1, all of which showed the phenomenon of photo inhibition. The fitting coefficient of four formulas R2 > 0.99, the optical response parameter index was the closest to the measured value on the right-angle hyperbolic correction model, with RMSE and MAE were closest to 0. Formula Ⅲ had a higher initial quantum efficiency(0.0914 μmol·μmol-1) and dark respiration rate(4.3167 μmol·m-2·s-1); Formula Ⅳ had higher maximum net photosynthetic rate(18.1636 μmol·m-2·s-1) and light saturation point(4199.4100 μmol·m-2·s-1). Two principal components with characteristic root >1 was extracted by PCA and their cumulative variance contribution rate reached 92.983 %.【Conclusion】Right angle hyperbola correction model had the best effect and formula 2# had the highest composite score, which was not only outstanding in low light environments, but also outstanding in high light environment. And they have high efficiency of light energy utilization, suitable for application in production. [ABSTRACT FROM AUTHOR]

Razor clam (Sinonovaculaconstricta) is one of the most commercial clam species in China, which is in the Siliqua genus with narrow and slender shells. It has been reported that razor clams are rich in vitamins, amino acids and carbohydrates, and have high nutritional value. However, razor clam is easy to rot during storage, which seriously affects its sensory quality and consumer preferences. Particularly, its abundance of nutrients can lead to high reproduction of microorganisms, thus shortening its shelf life. Therefore, it is highly necessary to propose a novel and efficient sterilization method inhibiting microbial contamination for razor clam preservation. Currently, the anti-microbial photodynamic technology is considered as a novel non-thermal sterilization technology that not only can reduce the microbial growth in foods, but also can preserve their nutritional value, color, quality, and water content. Therefore, the purpose of this study was to investigate the influence of anti-microbial photodynamic technology on the preservation of razor clam. The treated conditions of the anti-microbial photodynamic sterilization for razor clam preservation were optimized by total number of bacterial colony with Box-Behnken experimental design. Meanwhile, sensory evaluation and physicochemical indexes were also evaluated on razor clam after storage at 4℃ for 8 days. The results demonstrated that curcumin-mediated could effectively inhibit the residual bacterial contamination in the razor clam and delay the growth of bacteria. The curcumin concentration, soaking time and LED exposure time were the key factors affecting the antibacterial efficiency of this photodynamic sterilization. The sterilization rate could reach 99.99%, when photodynamic sterilization using 26.6 μmol/L curcumin concentration with 24.8 min soaking time and 20.5 min LED exposure time. In addition, the appearance, color and other sensory characteristics of razor clam were maintained in good condition after anti-microbial photodynamic technology treatment storaged at 4 ℃ for 6 days. Compared with the control group, the physicochemical indexes water content, pH value, thiobarbituric acid and total volatile basic nitrogen values of razor clam were also in desirable range after the optimized anti-microbial photodynamic treatment. The water content of the anti-microbial photodynamic -treated samples was 73.79%, which is significantly higher than that of the control group 59.94%, indicating that treatment of razor clam with curcumin-mediated anti-microbial photodynamic technology might also maintain the water content. In addition, within 6 days of storage, the increase in pH value and decreases in thiobarbituric acid and total volatile basic nitrogen values of razor clam were observed. Moreover, anti-microbial photodynamic technology treatment could significantly delay these changes compared to the control group. In conclusion, this study suggested that the anti-microbial photodynamic technology had good bactericidal activity and could be used for the preservation of razor clam. [ABSTRACT FROM AUTHOR]

Aiming at distinguishing service credibility in the large-scale distributed cloud computing system, we propose a relevance-aware cloud service trust model based on convex evidence theory. The model formallydescribes the trust relationship among service providers, services and users in the cloud computing system. Then, the correlation between different services from the same service provider is re-vealed. Furthermore, the model uses the convex evidence theory to deal with ordered propositions and finally builds a credible service recommendation method for the cloud computing system, which can pro-vide reasonable and reliable cloud services for users according to user needs. Through the comparison with the classical evidence theory, the experimental results demonstrate the validity and robustness of the relevance-aware cloud service trust model based on convex evidence theory while can make full use of correlation information among cloud services in the cloud computing system and provide reasonable cloud services according to user requests. [ABSTRACT FROM AUTHOR]

Objective To detect the acetylation of decorin (DCN) and its influence on DCN ubiquitination in mesangial cells in rats. Methods Mesangial cells of rats were cultured in vitro. The immunoprecipitation, Western blot assay and RT-PCR were used to determine the acetylation of DCN. Results DCN was acetylated in renal mesangial cells in rats. The acetylated DCN promoted its stability via inhibiting of its degradation through polyubiquitination. Moreover, transforming growth factor-β1 (TGF-β1) and type Ⅳ collagen expression of mesangial cells decreased, and cell growth was inhibited when acetylation of DCN was enhanced in mesangial cells. Conclusions Acetylation of DCN inhibited DCN ubiquitination degradation, which enhances DCN's antagonistic effect against nephritis. These results may provide a potential target for further study of prevention and treatment of mesangial cell proliferative glomerulonephritis. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of mindfulness based stress reduction on irritable bowel syndrome. Methods: PubMed, EMBASE, Cochrane Library, Web of Science, CNKI, Wanfang Data was searched and randomized clinical trials of MBSR treatment in irritable bowel disease were involved. At last two reviewers exracted the data independently . According to Cochrane Handbook5.1.0 handbook, a quality assessment was made. All the data would be analyzed with meta-analysis by the software of Stata. Results : Totally 5 randomized controlled trials were eligible, including 461 participants. Meta-analysis showed that MBSR would be better therapy method to improve the symptom severity of irritable bowel syndrome in 3 months, [ SMD=-0.60, 95% CI:-0.86 ~ -0.34, P<0.01]，and the pain index at the 3 months and 6 months was relieved compared to the baseline [ SMD=-1.1, 95%CI:-1.45 ~ -0.76, P<0.01, SMD=-0.14, 95% CI:-0 .43 ~ -0.14, P<0.05]. Conclusion : Mindfulness based stress reduction therapy could be a adjunctive therapy method in treatment of irritable bowel syndrome. [ABSTRACT FROM AUTHOR]

[Objective] In this paper, the change of water consumption during the growth period of Ziziphus jujuba in full fruit period under conventional flood irrigation was studied to prepare the water saving irrigation of jujube fruit period. [Method] Under flooding conditions, the flourishing gray jujube sap flow was monitored to understand the sap flow. [Result] During jujube fruit period throughout the growing season, the total sap flow maximum of enlargement period was 2367 218. 86 g, accounting for 40.46 % of the total growing season sap flow, and the total sap flow minimum of defoliation period was 197 991.5 g, accounting for 3.38% of sap flow in the total growing season; The sap flow of each growth period had a positive correlation with solar radiation and temperature, a negative correlation with air humidity, and both significant at 0.01 level. According to comprehensive evaluation, the main meteorological factors affecting sap flow at various growth stages were solar radiation, temperature and humidity. [Conclusion] Depending on the dominant factors in stem flow of the different growth stages, the reasonable irrigation system would be established to provide a theoretical basis for the flourishing gray water-saving irrigation of the late date. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of megsin siRNA plasmid on the proliferation of glomerular mesangial cells and matrix Thy1 glomerulonephritis in rats. Methods Nine male SD rats were equally and randomly divided into 3 groups: A group was control group; B group was Thy1 glomerulonephritis model group; C group was megsin siRNA plasmid group which was the Thy1 glomerulonephritis model with renal artery injection of megsin siRNA plasmid, supplemented by electroporation technology. Kidney specimens were examined by real-time PCR,HE staining, PAS staining,and immunohistochemistry staining for megsin and PCNA.Results Compared with the A group, the HE staining results showed that the glomerular mesangial cells significantly increased with the increase of matrix in B group, while the number of glomerular cells in the C group was significantly less than that in the B group, but little more than in the A group; PAS staining showed that the matrix of glomerular mesangial cells significantly increased and segmentally distribution in B group, while it was decreased in C group; the results of RT-PCR and immunohistochemistry analysis showed that the megsin mRNA and protein levels in glomeruli increased significantly in B group compared with A group (P＜0.01), while both decreased significantly in C group compared with B group (P＜0.01) and A group (P＜0.05). The average number of PCNA positive nuclei was 3 in A group, 14.79 in B group, while 6.94 in C group, respectively. Statistics showed that there were significantly different between the B group and A group (P<0.001), and B group and C group (P＜0.001). Conclusions The proliferation of mesangial cells and matrix were inhibited after the injection of megsin siRNA plasmid and nephritis lesions reduced, which suggested that in vitro transfection of megsin siRNA plasmid in rat Thy1 nephritis model has antagonistic effect on glomerulonephritis. It provides a new idea to explore the treatment and prevention of renal diseases with mesangial cell proliferation. [ABSTRACT FROM AUTHOR]

To study Aksu Jujube's photosynthetic characteristics differences of mature gray leaf maturity date change with diameter class being ( 12cm ± 0 .5 cm)， ( 18cm ± 0 .5 cm) in pit irrigation and flood conditions，Li6400 photosynthesis system was adopted under cloudless weather and the net photosynthetic rate (Pn)，stomatal conductance (Gs)，intercellular CO2 concentration (Ci)， transpiration rate (Tr) and other photosynthetic indexes were measured. Results showed that (i) the diurnal variation of net photosynthetic rate of 4 experiments (Pn)，stomatal conductance (Gs)，transpiration rate (Tr) showed a clear bimodal curve，13 : 00 - 15 : 00 significantly midday depression. (ii) Four experiments WUE had no significant difference. (iii) Gs was the main impact factor for the 4 experiments and PAR was the most important factor of 4 experiments Tr. It was concluded that Pit Irrigation C1，C2 and flooding on photosynthesis C3，G4 photosynthesis were similar to pit under irrigation to meet a normal photosynthesis age of Jujube. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of Gumibao Recipe on lipid metabolism in Steroid-induced Necrosis of the Femoral Head (SINFH) in rats，in order to explore its partial mechanisms for treating Steroid-induced Necrosis of The Femoral Head． Methods Fifty SD rats were randomly assigned to five groups: blank control group，model group，Gumibao Recipe groups (High，medium and low-dose groups，10 rats in each group，with half male and half female)． SINFH models were established according to S． Okazaki's technique． All the interventions lasted for 4 weeks by gavage． The data on lipid metabolism were determined． Results Compared with the model group，Gumibao Recipe could reduce the serum levels of TC，TG，LDL and enhanced the level of HDL (P ＜ 0. 05)． TEM ultrastructure observation showed that compared with the model group，the bone cell morphological of Gumibao recipe was better． Conclusion Lipid metabolism disorder is closely related to SINFH，and Gumibao recipe could regulate lipid metabolism，improve dyslipidemia，and delay the development of SINFH． [ABSTRACT FROM AUTHOR]

The U. S. A production of Li-6400 portable plant light and measuring instrument, with eight different varieties of jujube tree as the research object, the jujube leaf net photosynthetic rate (Pn), stomatal (Cond), transpiration rate (Tr), intercellular CO2 concentration (Ci) etc. photosynthetic indexes were determined and com PARed. The results showed that: The change of net photosynthetic rate of jujube varieties showed two peak curve, the net photosynthetic rate of daily average performance: ‘Zanhuangdazao' > ‘Bianhesuan' > ‘Yuancui' > ‘Jinzao'‘ Jixin' > ‘Huizao' > ‘Banzao' > ‘Jinsixiaozao', between the two peak period had obvious photosynthetic "midday depressions" phenomenon; transpiration rate of average performance was: ‘Zanhuangdazao' > ‘Bianhesuan' > ‘Jinzao'‘Yuancui' > ‘Jixin'‘ Huizao' > ‘Banzao' > ‘Jinsixiaozao'; water use efficiency on average performance is ‘Yuancui' > ‘Bianhesuan' > ‘Huizao' > ‘Jinsixiaozao' > ‘Jixin' > ‘Banzao' > ‘Zanhuangdazao' > ‘Jinzao'; The minimum values of CO2 concentration in each date were different, but the maximum values appeared in the early morning. Through the analysis of all varieties of jujube tree clustering that, ‘Banzao' and ‘Jinsixiaozao' belong to the low Pn, Tr and low WUE varieties; ‘Zanhuangdazao', ‘Bianhesuan' and ‘Jinzao' belong to high Pn, Tr and low WUE jujube cultivars; ‘Jixin', ‘Yuancui' and ‘Huizao' belong to the low Pn, high WUE and low Tr jujube cultivars, more suitable for cultivation under the environment of drought and water shortage around oasis in Tarim Basin. [ABSTRACT FROM AUTHOR]

Aksu area of irrigation water resources use rate is relatively low, the irrigation, drip irrigation, and arid environment and water resource shortage have become the main factors of constrainting local jujube planting industry development, therefore, it is urgent to find new water-saving irrigation method. We studied the relationship between jujube stem flow variation by different irrigation methods and meteorological factors, continuously monitored stem flow rate with Flow-32 packaged stem sap flow gauge produced by Dynamax company. The results showed that the change of stem linger under two kinds of irrigation methods presenting the obvious diurnal variation law of stem flow rate were unimodal curve and stem flow activities at night phenomenon was obvious. Stem flow morning starting time of was 0.5 h earlier than that of W2, W1 and W2 jujube stem flow were small amplitude “peak population” before and after the peak, W1 and W2 peak time were respectively 13:30-14:30 and 13:00-14:30, peak value were 2 133.33-2 724.93 g·h-1 and 2234.11-2 689.40 g·h-1. Stem flow daily cumulative quantity change process curve were “S” shape, but W1, stem flow was greater than the total W2; Stem flow rate variation curves of solar radiation and temperature showed a positive correlation, a negative correlation with air humidity, poor correlation with wind speed; The apparent wave solar radiation trends under two irrigation methods and stem the flow curves presented similarities and differences, the start time was 0.5 h earlier than that of W1, 1 h earlier than that of W2, but the peak time was later than W1 and W2. Through correlation analysis, we deduced that the solar radiation main influencing factors, and set up two kinds of irrigation mode flow rate and meteorological factors of multivariate linear model, through the regression coefficient and correlation coefficient test, W1 and W2 multiple linear regression equation reached the significant level. This study provides scientific information for the practicality and superiority of new irrigation method W1. [ABSTRACT FROM AUTHOR]

In the gunny windless weather, using Li-6400 portable photosynntheses system, the diurnal change of photosynthetic rate(Pn) , stomata] conductance ( Gs ) , intercellular C02 concentration ( Cj ) , transpiration rate(Tr) , water use efficiency ( WUE) and other physiological indicators of young Zizipkus jujuba cv. Huizao [ (3 ±0. 5) cm ground diameter] under two different irrigation treatments [well type irrigation (W1,irrigation volume 60 L one time) and drip irrigation (W2, irrigation volume 72 L one time) in Aksu] were measured, and the effects of different irrigation treatments on its photosynthetic physiological indicators were studied. The results showed that the averages of Pn ,CS ,Ci ,Tr had no significant differences under W1 ,W2 treatments in the diurnal courses, but the WUE under W1 treatment was significantly higher than that under W2 treatment. To maintain fixed photosynthetic product capacity, the young Zizipkus jujubacv. Huizao need to reduce the ground evaporation and improve water use efficiency under W1 treatment- This research suggested that the W1 treatment would be a way of Zizipkus jujubacv. Huizao water saving irrigation, which would provide the theoretical basis of precise irrigation for young Zizipkus jujubacv. Huizao in Aksu. [ABSTRACT FROM AUTHOR]

目的 探讨 ERK 信号通路对急性 ST 段抬高型心肌梗死患者急诊 PCI 术后冠脉无复流的影响。方法选择急性 ST 段抬高型心肌梗死（STEMI）行急诊 PCI 的患者38例，根据术后 TIMI 分级分为无复流患者16例（无复流组）和有效灌注者22例（再灌注组）；另选择20例健康体检者作为对照组。采用全自动生化分析仪测定各组血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇、尿素氮、肌酐、尿酸等生化指标，利用 RT-PCR 方法测定各组外周血单个核细胞 ERK mRNA 及 bad mRNA 表达。结果无复流组、再灌注组血清 TG、TC、LDL-C 水平明显高于对照组（P 均＜0．05）；无复流组血清 LDL-C 水平明显高于再灌注组（P ＜0．05）。对照组单个核细胞 ERK mRNA 表达水平高于无复流组及再灌注组，bad mRNA 表达水平低于无复流组及再灌注组（P 均＜0．05）；无复流组 ERK mRNA 表达水平低于再灌注组，bad mRNA 表达水平高于再灌注组（P 均＜0．05）。单个核细胞 ERK mRNA 与 bad mRNA 表达水平呈负相关（r ＝－0．61，P ＜0．05）。结果在 STEMI 患者急诊 PCI 术无复流的患者中 ERK 信号通路表达活性下降；ERK 信号通路活性降低及 bad mRNA 表达增强可能参与了急诊 PCI 术后冠脉无复流的发生。 [ABSTRACT FROM AUTHOR]