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A zinc complex [Zn2 (Hbppc)(bppc)Cl3]·0. 5H2 O was synthesized under hydrothermal conditions using 4-(2,6-di(pyrazin-2-yl)pyridine-4-yl)-benzoic acid (Hbppc) as ligand and ZnCl2 as metal source. The complex was characterized by SXRD, PXRD, IR, FT-IR, etc. X-ray single crystal diffraction shows that the complex crystallized in the orthorhombic, Pbca space group, with a zero-dimensional structure. Moreover, the complex has a two-nuclear structure, and the binuclear units are further connected by hydrogen bonds to form 2D supramolecular network structure. Hirshfeld surface analysis of the complex was performed by Crystal Explorer 21. In addition, fluorescence analysis of ligands and the complex were performed, and the results show that the fluorescence of the complex is significantly enhanced compared with that of the free ligand. Gaussianview 5. 0 and Gaussian 09W were used to calculate the frontier molecular orbital energy of ligand and complex, and to explore the mechanism of fluorescence enhancement. [ABSTRACT FROM AUTHOR]

A new nickel complex [Ni(dpyb)2 ](NO3)2 ·(H2 O)3 was synthesized by means of solvent-thermal synthesis using 2,6-bis(2-pyrazinyl)-3,4′-bipyridine (dpyb), which is one of polynitrogen compounds, as the ligand and Ni(NO3)3 ·6H2 O as raw materials. The structure of the complex [Ni(dpyb)2 ](NO3)2 ·(H2 O)3 was characterized by X-ray single crystal diffraction, infrared spectroscopy and Raman spectroscopy. The results of structural analysis show that the complex was a mononuclear structure, and the mononuclear structure is further extended to the binuclear structure through intermolecular hydrogen bonds between the mononuclear units. The Hirshfeld surface analysis of the target complex was carried out using Crystal Explorer 21 software, and the results show that there is strong hydrogen bonding in the structure of [Ni(dpyb)2] (NO3)2 ·(H2 O)3. The frontline molecular orbitals and energy band gaps of the free ligand and the complex were also calculated by Gaussian 09W and Gauss View 5. 0 software using the B3LYP/6-311G (d, p) method, and the results suggest that the complex has high chemical stability. The results of the computational study are in good agreement with the experiments. In addition, fluorescence analysis of the free ligand and complexe were performed, and the results indicate that compared with the free ligand, the fluorescence intensity of the complex is weakened, which is due to the coordination between the ligand and the Ni(II) ion. [ABSTRACT FROM AUTHOR]

The introduction of tobacco has always been the focus of tobacco history research, but the previous studies have mainly focused on Nicotiana tabacum, and there were obvious deficiencies in the study of Nicotiana rustica. In fact, Nicotiana rustica was also an important part of tobacco species for human planting and use, its introduction had a profound impact on the society of that time and even later generations. Based on the collection and arrangement of the annals and anthology, this paper shows that the Nicotiana rustica had already been introduced in the period Emperor Chongzhen’s reign, no later than the period of Emperor Kangxi’s reign. It was introduced into China mainly through smuggling, normal trade and the diplomatic envoys’ spread. Central Asian merchants, Russian private merchants and diplomatic envoys, and the tribes of Mongolian all played an active role in varying degrees. [ABSTRACT FROM AUTHOR]

To analyze the prognostic factors of transarterial chemoembolization (TACE) combined with antiviral therapy for hepatitis B virus (HBV)-associated hepatocellular carcinoma. This study included 130 patients with HBV-associated hepatocellular carcinoma admitted to the hospital from February 2018 to March 2020. All patients underwent TACE combined with antiviral therapy, and their survival status was recorded. The prognostic factors were analyzed. At the end of follow-up, 58 patients died and 72 patients survived, with a median survival time of 24 months. The 1-year and 2-year survival rates were 82.31% and 55.38%. Univariate analysis and multivariate Cox regression analysis found that the maximum tumor diameter, serum AFP, Child-Pugh grade, abdominal cavity metastasis, and portal vein tumor thrombus were risk factors for poor prognosis. The maximum tumor diameter, serum AFP, Child-Pugh grade, abdominal cavity metastasis, and portal vein tumor thrombus are factors affecting the prognosis of HBV-associated hepatocellular carcinoma treated with TACE combined with antiviral therapy. [ABSTRACT FROM AUTHOR]

Objective·To elucidate the regulatory mechanisms of the chromobox protein homolog 8 (CBX8) in prostate cancer metastasis from transcriptome and epigenetic modification perspectives. Methods·The correlation between the expression of CBX proteins and prostate adenocarcinoma (PRAD) in The Cancer Genome Atlas (TCGA) was examined through an analysis based on cBioPortal database. A stable CBX8 knockdown DU145 prostate cancer cell line was established via short hairpin RNA (shRNA) transfection. Subsequently, the proliferation and invasion of the CBX8 knockdown cells were analyzed by CCK-8 assay and Transwell assay, respectively. Transcriptome changes of the CBX8 knockdown cells were investigated through RNA sequencing (RNA-seq) coupled with Gene Set Enrichment Analysis (GSEA). To further evaluate the functional implications of these transcriptomic alterations, Gene Ontology (GO) for functional analysis was deployed. Moreover, to identify potentially affected signalling pathways, the Kyoto Encyclopedia of Genes and Genomes (KEGG) was utilized for pathway enrichment analysis. Lastly, the levels of H3K27me3, a key histone modification associated with CBX8, in the knockdown cells were determined by chromatin immunoprecipitation sequencing (ChIP-seq). Results·Bioinformatic analysis with cBioPortal database, based on TCGA-PRAD cohorts, unveiled a high CBX8 mRNA expression in PRAD. Knockdown of CBX8 did not significantly affect the proliferation of DU145 cells (P>0.05), but caused a a significant increase in their invasiveness (P<0.05). The RNA-seq analysis revealed that CBX8 knockdown led to the upregulation of 750 genes and the downregulation of 951 genes. Notably, branched-chain-amino-acid aminotransferase 1 (BCAT1), a gene implicated in the metastasis of various types of cancers, showed a significant increase in expression following CBX8 knockdown. GSEA showed that the expression levels were of the affected genes were related to the functions of the polycomb repressive complex 1 (PRC1). A further investigation using GO and KEGG analyses identified several enriched pathways in the CBX8 knockdown cells, including transfer RNA (tRNA) aminoacylation, DNA replication, changes in aminoacyl-tRNA ligase activity, and cadherin binding. Interestingly, in terms of cell component of GO functional analysis, cellsubstrate junction-related genes associated with tumor metastasis appeared to be enriched. ChIP-seq results showed a global decrease in H3K27me3 levels. Significantly, 97 reduced H3K27me3 peaks were found located nearby genes that were upregulated upon CBX8 knockdown, including the transcriptional start site of BCAT1. Conclusion·CBX8 is highly expressed in prostate cancer. CBX8 suppresses prostate cancer cell invasion, possibly by recruiting the transcriptional repressive PRC1 complex to the transcription site of BCAT1, thereby inhibiting BCAT1 transcription and tumor metastasis. [ABSTRACT FROM AUTHOR]

Traditionally, the progression from compensated liver cirrhosis to decompensated liver cirrhosis has been considered an irreversible point in the natural history of the disease； however, with the suppression of underlying etiology, cure, and disease regression, this view is challenged by an increasing number of new evidence, and the idea of “recompensation of liver cirrhosis” is gradually being accepted. In recent years, scholars in China and globally have been exploring the specific definition of recompensation of liver cirrhosis and the clinical features of patients. By summarizing the recent studies on recompensation of liver cirrhosis in China and globally, integrating existing views, and analyzing related research evidence, this article points out the main challenges in the field of recompensation at this stage, including the lack of in－depth clinical and basic research, the need to define recompensation in the context of NAFLD, and related ethical issues, in order to provide new directions for future research in this field. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship between preoperative prognostic nutrition index (PNI) and prognosis of patients with lung squamous cell carcinoma and its predictive effect on postoperative recurrence and death. Methods: A total of 78 patients with lung squamous cell carcinoma who received treatment in our hospital from January 2017 to January 2019 were included. All patients had complete clinical data and pathological information, and they were reviewed and followed up in the outpatient department for 3 years. In addition to the lost cases, 76 patients were included, during the period, 43 patients recurred and 37 patients died. The 76 patients were divided into recurrence group (n=43) and non-recurrence group (n=33), death group (n=37) and survival group (n=39) according to the recurrence and death conditions. The independent risk factors affecting the recurrence and death of patients with lung squamous cell carcinoma were analyzed by univariate and multivariate Logistic regression. The receiver operating characteristic (ROC) curve was used to analyze the predictive efficacy and optimal cutoff value of PNI in the postoperative recurrence and death of patients with lung squamous cell carcinoma. Results: Univariate analysis showed that TNM stage, smoking years, diabetes, family history, PNI were the related factors affecting the postoperative recurrence of patients with lung squamous cell carcinoma (P<0.05). Gender, age, TNM stage, BMI, smoking history, smoking years and PNI were the related factors affecting the postoperative death of patients with lung squamous cell carcinoma (P<0.05). Multivariate Logistic regression model analysis showed that TNM stage III, long smoking years and family history were independent risk factors for postoperative recurrence of patients with lung squamous cell carcinoma, and PNI was protective factor (P<0.05). In addition, male, older age, TNM stage III, and longer smoking years were independent risk factors for postoperative death of patients with lung squamous cell carcinoma, and PNI was the protective factor (P<0.05). ROC results showed that the area under curve of PNI in predicting postoperative recurrence of patients with lung squamous cell carcinoma was 0.746 the sensitivity was 83.72%, the specificity was 63.64%, and the best cutoff value was less than or equal to 49.90. The area under curve of PNI in predicting postoperative survival of patients with lung squamous cell carcinoma was 0.761, the sensitivity was 86.49%, the specificity was 66.67%, and the best cutoff value was less than or equal to 50.41. Conclusion: PNI has high predictive efficacy for postoperative recurrence and survival of patients with lung squamous cell carcinoma, increasing PNI level has a positive effect on improving the prognosis of patients with lung squamous cell carcinoma. [ABSTRACT FROM AUTHOR]

[Objectives] This experiment was conducted to study the effects of malondialdehyde(MDA)on protein oxidation of soybean meal and the alleviation of tea polyphenols(TP). [Methods] Fresh soybean meal was divided into eight treatment groups, which was treated with 0,0.1,1 and 10 mmol·L-1 MDA, and also treated with 0,0.1,1 and 10 mmol·L-1 MDA and 0.6 mg·mL-1 TP, respectively, and the contents of carbonyl, sulfhydryl, and amino acid in soybean meal were determined. [Results]With the increasing concentration of MDA, the carbonyl content of soybean meal gradually increased(P<0.05),whereas the sulfhydryl and total sulfhydryl groups content, methionine, tyrosine, lysine contents, and in vitro digestibility of soybean meal protein decreased accordingly(P<0.05),the intrinsic fluorescence intensity of tryptophan increased first and then decreased, and its max peak was gradually blue-shifted, accompanied by an increase in the fluorescence intensity of Schiff base. In the oxidative group with the MDA concentration of 1 mmol·L-1, the carbonyl content in soybean meal significantly increased(P<0.05), the contents of sulfhydryl and total sulfhydryl groups significantly decreased(P<0.05),and the in vitro digestibility of soybean meal protein significantly decreased(P<0.01). In the high concentration MDA group (10 mmol·L-1),the carbonyl content in soybean meal significantly increased(P<0.01),and the contents of sulfhydryl and total sulfhydryl, the in vitro digestibility of soybean meal protein significantly decreased(P<0.01). Meanwhile, the methionine, tyrosine, lysine content in soybean meal were remarkably lower than those of the control group(P<0.05). TP addition could mitigate the increase of carbonyl content, the decrease of sulfhydryl and total sulfhydryl groups, the decrease of methionine content, the decrease of in vitro digestibility of soybean meal protein, the decrease of intrinsic fluorescence intensity of tryptophan and the increase of Schiff base content in soybean meal. [Conclusions]1 mmol·L-1MDA can trigger protein oxidation in soybean meal, resulting in a large change in the structure of soybean meal protein and the reduce in vitro digestibility of soybean meal protein. At this time,TP can effectively alleviate the MDA-induced protein oxidation in soybean meal and the reduced in vitro digestibility of soybean meal protein. [ABSTRACT FROM AUTHOR]

目的 观察苦参碱对于人结肠癌细胞株HT-29增殖及p53蛋白表达的影响，探讨中药苦参治疗结肠癌的 作用机制。方法 选取人结肠癌细胞株 HT-29，用IL-6处理造模后加不同浓度苦参碱干预，并设置空白对照组，检 测IL-6处理模型组及不同浓度苦参碱干预各组HT-29细胞活力及p53蛋白表达情况。通过网络检索TCMSP、OMIM 和 Disgenet三个数据库得到苦参主要活性成分及对应疾病的靶点，运用 String数据库及 Cytoscape 软件将苦参对结 肠癌的潜在靶点构建PPI，将药物疾病共同基因导入Metasacpe数据平台进行GO和KEGG富集分析。结果 人结肠 癌细胞株 HT-29模型组细胞活力较空白组增强，p53蛋白表达水平下降；经苦参碱干预后各组细胞活力明显降低， p53蛋白表达水平增高。通过网络检索数据库共检索出苦参的45个有效成分和22个潜在作用靶点，检索出的治疗 通路主要有VEGF信号通路、癌症通路、晚期糖基化产物（AGE）-晚期糖基化终末产物受体（RAGE）信号通路等。结 论 苦参能抑制结肠癌细胞增殖，并提高抗肿瘤基因p53蛋白的表达；其机制为苦参中抗癌活性成分通过多种信号 通路作用于p53、IL-6、AKT1、PTGS2等靶点，从而对结肠癌起到干预作用。 [ABSTRACT FROM AUTHOR]

The photoelectric properties and bond lengths of 2D CuI with intrinsic defects are calculated based on density function theory, and the energy band structures, densities of states and complex dielectric functions a re analyzed in detail. The bandgap of intrinsic 2D CuI is 1. 56 eV, and it is a direct bandgap semiconductor material. The introduction of I and Cu defects reduce the bandgap of intrinsic 2D CuI, and the introduction of Cu defect does not change the bandgap mode of 2D CuI, while the 2D CuI modified by I defect becomes an indirect bandgap semi conductor. The results of optical properties showth at the static dielectric function of intrinsic 2D CuI is 2. 47, the introduction of I defect has little effect on the static dielectric function of 2D CuI, but the static dielectric function of 2D CuI increases sharply when Cu defect is present. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to analyze the composition of arbuscular mycorrhiza(AM) fungi in the rhizosphere soil of healthy and diseased ginger plots to provide a theoretical basis for the comprehensive control of ginger blast using micro-ecological control methods.[Method] Using ginger fields with different disease severities in Guilin, Liuzhou and Baise, ten soil samples were collected from the following fields: Guilin Quanzhou, Liuzhou Sandu, Liuzhou Liucheng, Baise Debao and Baise Tianlin. Healthy and diseased ginger numbers are KJJ2S,BMJ1S,LCJ1S,FGJS,NGJS,KJB2S,BMB1S,LCB1S,FGBS and NGBS. Illumina Miseq high-throughput sequencing technique was used to study the diversity and community structure of AM fungi in the soil. The correlation between fungal community structure and soil nutrients was clarified with redundancy analysis (RDA). [Result] At the similarity level of 97%,the OTU number and Chao index of fungi in diseased samples were higher than those of healthy samples. By evaluating the diversity index of the AM fungi species through the Shannon index and Simpson index, it was found that the healthy samples had a higher diversity index compared to the diseased samples in all the sample fields. In terms of community composition at the genus level, 312 OTUs are classified into Paraglomus, Acaulospora, and Glomus and unclassified cGlomeromycetes. RDA results showed that soil available phosphorus significantly affected the AM fungal community (P<0.05, the same as below), and total nitrogen, organic matter, pH, and Magnesium(Mg) had no significant effects on the AM fungal community(P>0.05).The results of Heatmap analysis showed that Paraglomus was significantly negatively correlated with available phosphorus content(P<0.01), and significantly negatively correlated with organic matter content. [Conclusion] There are differences in the composition of AM fungal community between ginger healthy plots and diseased plots. The OTUs number and abundance of AM fungi of diseased ginger soil are higher than healthy ginger soil, but less diversity than healthy ginger soil. Dominant species of AM fungi at different sampling points are different, and their diversity, abundance and community composition structure are mainly affected by effective P content. [ABSTRACT FROM AUTHOR]

The annealing was used to remove the adsorbed gases and impurities on graphene film, and the state of graphene surface was changed. The surface of graphene film before and after annealing was characterized in situ by atomic force microscope and Kelvin probe force microscope. The surface morphology and surface potential map of graphene film before and after annealing were obtained respectively. The work function was calculated according to the surface potential map, and the change mechanism of work function induced by annealing was analyzed. The results show that the annealing treatment causes the escape of water molecular between the graphene film and substrate, which reduces the gap between graphene film and SiO2 substrate and the P-type doping level of graphene film, resulting in the increasing of the Fermi level and the decreasing of work function. [ABSTRACT FROM AUTHOR]

【Objective】Histone H1 plays an important role in the maintenance and stabilization of higher chromatin structure. Elucidating the effect of rice histone H1 on gene expressions will help to better understand the regulatory functions of H1 in rice.【Method】 Semi-quantitative RT-PCR and real-time quantitative PCR were used to detect the expressions of four H1 genes in rice. CRISPR technology was used to generate h1 mutant plants; subsequently, phenotypic and transcriptomic analysis of the h1 mutant was performed.【Result】The four rice H1 genes were broadly expressed, and are weakly expressed in root. In T0 generation of CRISPR-based gene editing plants, multiple mutations in H1.1-H1.4 were identified. In T1 generation, one Osh1.1 Osh1.3 Osh1.4 homozygous triple mutant was identified, which had various developmental defects and became the material for further transcriptomic sequencing analysis. In T2 generation, quadruple and triple mutants were acquired, approximate 25% of which were albino seedlings, slow in plant development and defect in drought responses. By transcriptome sequencing of the h1 mutant, 1 055 differentially expressed genes were identified, of which the significantly up-regulated genes were approximately 2.5 times of the down-regulated genes, implying that H1 may inhibit gene expressions at genome-wide scale.【Conclusion】 In the mutant, pathways of photosynthesis, stress responses, amino acid and RNA metabolism were disrupted. The ribosome biogenesis and photosynthesis pathway genes were significantly up-regulated; while the stress-associated dehydrogenase genes were down-regulated. It is presumed that overexpression of ribosome pathway genes resulted in the disorder of protein homostasis, which ultimately caused plant developmental defects. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the efficacy of gefitinib combined with oxaliplatin in treatment of gastric cancer and its effects on the expression of epidermal growth factor receptor(EGFR) and systemic inflammatory response syndrome(SIRS). METHODS: 120 patients with gastric cancer admitted into the First Hospital Affiliated to Army Medical University of PLA from May 2017 to Aug. 2018 were selected and divided into control group and observation group according to different therapeutic regimens, with 60 cases in each group. The control group was treated with oxaliplatin alone, while the observation group was given gefitinib combined with oxaliplatin. Differences in short-term efficacy, incidences of adverse drug reactions, mutation rates of EGFR gene and incidences of SIRS of both groups were compared. RESULTS: The short-term total effective rate of observation group was 75.00%(45/60), which was significantly higher than that of the control group(48.33%, 29/60), with statistically significant difference(P<0.05); during treatment, the incidence of adverse drug reactions of observation group was 61.70%(37/60), which was slightly higher than that of the control group(58.33%, 35/60), the difference had no statistical significance(P>0.05); the mutation rate of EGFR of observation group was 63.33%(38/60), which was significantly higher than that of the control group(26.67%, 16/60), with statistically significant difference(P<0.05); in the two groups, the total effective rate of patients with mutations was significantly higher than those without mutations, with statistically significant difference(P<0.05); during treatment, the incidence of SIRS of observation group was 41.67%(25/60), which was slightly higher than that of the control group(35.00%, 21/60), the difference had no statistical significance(P>0.05). CONCLUSIONS: Compared with oxaliplatin alone, the efficacy of gefitinib combined with oxaliplatin in treatment of gastric cancer is better, with higher mutation rate of EGFR gene, few effects on incidences of adverse drug reactions and SIRS. [ABSTRACT FROM AUTHOR]

Objective: To assess the clinicopathological benefit of bevacizumab (Bev.) in neoadjuvant chemotherapy (NAC) for rectal cancer patients and to investigate its influence on microvessel status in cancerous tissue. Methods: We retrospectively analyzed the data of 47 rectal cancer patients who received NAC with or without Bev. The objective response was evaluated using the maximum tumor diameter. Tumor regression grade was classified as a pathological response. Results: Thirty-one patients (66%) received NAC including Bev, as the Bev group; and the other 16 patients were treated without Bev, as the non-Bev group. The objective response rate was significantly higher in the Bev group than in the non-Bev group (64.5%vs.25.0%, p=0.015). The rate of pathological response was higher in the Bev group (41.9%) than in the non-Bev group (12.5 %, P=0.052), but difference was not statistically significant. The microvessel density (MVD) of the resected cancerous tissue was significantly lower in the Bev group than in the non-Bev group. Conclusions: The objective and pathological responses were better in patients treated with NAC plus Bev than in those who received NAC without Bev. Additionally, MVD in tumor tissues was inhibited in the patients treated with NAC plus Bev. [ABSTRACT FROM AUTHOR]

[Objective] The heading date of rice（Oryza sativa）is an important agronomic trait and crucial for the regional adaptability and yields. Identification and functional analysis of heading date-associated genes may provide important gene resources for the breeding in rice. [Method] BSA-seq was performed to identify target gene of a yellowish and early heading mutant huangzao 1（hz1）. RT-qPCR was used to analyze the expression profile of target genes. The subcellular localization of HZ1 was determined by transient transformation between rice protoplasts and tobacco cells. Meanwhile, the heading dates, chlorophyll content and hydrogen peroxide content were measured to analyze the phenotype of hz1 mutant. [Result] Field phenotypic observations found that hz1 showed early heading phenotype. The heading date of hz1 mutant was the same under long-day（LD）and short-day（SD）conditions, which were 43 d and 26 d earlier than those of wild type（WT）respectively, indicating that hz1 was a photoperiod-insensitive mutant. Moreover, hz1 demonstrated a yellowish phenotype, with a decrease in chlorophyll content compared with WT. Genetic analysis revealed that hz1 was controlled by a recessive gene. BSA-seq（Bulk segregation analysis with whole-genome sequencing）of F2 pools indicated that the causal gene was linked with molecular markers on the chromosome 6 with 17.8 Mb region. Further analysis demonstrated that LOC_Os06g40080 site with a T-DNA insertion was completely linkage with hz1 mutant. LOC_Os06g40080 site was an identified SE5 gene encoding heme oxygenase 1（HO1）. Expression pattern analysis revealed that HZ1/SE5 was highly expressed in the leaves and dhad diurnal rhythm expression. Subcellular localization assay showed that HZ1/SE5 protein was localized in chloroplasts. Gene expression analysis showed that HZ1/SE5 regulated the expression of florigen genes Hd3a and RFT1 to modulate the heading date in rice. In addition, HZ1/SE5 can also regulate the expression of genes associated with chlorophyll synthesis pathway to modulate the change of chlorophyll levels. [Conclusion] hz1 mutant is a photoperiodic insensitive mutant due to the mutation of heme oxygenase gene SE5. HZ1/SE5 may regulate the expression of the florigen genes and chlorophyll synthesis-associated genes to influence the heading date and leaf yellowish in rice. [ABSTRACT FROM AUTHOR]

The geometric structures and optoelectronic properties of intrinsic 2D CuI and Co and Fe doped 2D CuI are systematically studied by using on-the fly generated (OTFG) ultrasoft based on density function theory. The results show that the band gap of intrinsic 2D CuI is 1.61 eV. Co doping in 2D CuI introduces two shallow donor energy levels as well as two deep donor energy levels, and the introduction of shallow energy levels increases the concentration of carriers. Fe doping introduces two deep donor energy levels, which means that Fe doping does not affect the conductivity of 2D CuI. The results of optical properties show that the static dielectric constant of 2D CuI is 2.42, the doping of Co and Fe increase the static dielectric constant of 2D CuI and improves the light absorption characteristics of 2D CuI in the visible range and decreases the 2D CuI transmission coefficient. [ABSTRACT FROM AUTHOR]

目的探讨护理干预对老年普外科手术患者症状及自我效能的影响。方法选取2014年1月至2016年 12月中国人民解放军第152中心医院普外科收治的180例老年手术患者作为研究对象，采用随机数字表法将患者分为干 预组和对照组，各90例。对照组患者采用一般常规护理，干预组采用系统性护理干预，采用汉密尔顿抑郁量表（HAMD)和 汉密尔顿焦虑量表（HAMA)评价患者症状，采用汉化Anderson慢性疼痛自我效能感量表（CPSS)评价患者自我效能，比较 两组患者临床症状及自我效能的变化情况。结果与对照组干预后比较，干预组HAMD评分[(13.63±2.18)分]、HAMA评 分[(15.42±2.26)分]均明显降低，疼痛管理评分[(16.15±2.28)分]、躯体功能评分[(32.46±4.13)分]、症状应对评分[(27.35± 3.86)分]及总分[(75.12±8.85)分]均明显提高，差异均有统计学意义(！<0.05)。结论系统性护理干预对老年普外科手术 患者临床症状及自我效能的改善均具有十分重要的意义。 [ABSTRACT FROM AUTHOR]

To investigate the potentially inappropriate medication ( PIM )and influencing factors in elderly patients in cardiology, and to explore the medication crisis management measures for elderly patients in cardiology. METHODS: A total of 300 elderly patients were randomly selected from the cardiology department of General Hospital of Eastern Theater Command of Chinese People’s Liberation Army from Aug. 2019 to Nov. 2020.PIM was evaluated according to STOPP/START criteria (2014 Edition）, and the influencing factors of PIM in elderly patients in cardiology were analyzed by Logistic regression. RESULTS: Among the 300 patients, there were 235 cases of PIM, 104 case-times with STOPP criteria and 271 case-times with START criteria, and there were cases of PIM combined with multiple STOPP/START criteria. Univariate analysis showed that age, number of diseases, drug combination, history of cardiovascular diseases and antithrombotic therapy were correlated with the occurrence of PIM in elderly patients in cardiology (P <0. 05) . Logistic regression analysis showed that atrial fibrillation, stable angina, coronary atherosclerosis, drug combination and antithrombotic therapy were risk factors for PIM (P < 0. 05) . CONCLUSIONS: The influencing factors of PIM in elderly patients in cardiology mainly include atrial fibrillation, stable angina, coronary atherosclerosis, drug combination (from 11 to 21） and antithrombotic therapy. Medication crisis management measures such as standardizing the classification and storage of drugs, improving cardiology medication according to STOPP/START criteria, and standardizing the cardiology medication process are of positive significance in reducing the risk of PIM in elderly patients in cardiology. [ABSTRACT FROM AUTHOR]

目的 观察侧脑室注入网膜素（omentin）对小鼠摄食量及其下丘脑组织神经肽 Y（NPY）、刺鼠相关蛋白（AGRP）、促肾上腺皮质激素释放激素（CRH）、阿片-促黑素细胞皮质素原（POMC）mRNA表达的影响。方法 20只昆明小鼠随机分为实验组及对照组,两组侧脑室置管并保留72 h后分别于侧脑室注射omentin及生理盐水（1.2μg/kg）,观察注射后24 h小鼠摄食量有无变化,RT-PCR检测两组小鼠下丘脑组织AGRP、NPY、CRH及POMC mRNA。结果实验组、对照组摄食量分别为（4.63±0.17）、（4.51±0.12）g,两组比较,P〉0.05。实验组、对照组NPY mRNA相对表达量分别为0.47±0.24、0.48±0.29,AGRP mRNA分别为24.31±6.21、22.01±7.14,POMC mRNA分别为0.92±0.48、0.83±0.16,CRH mRNA分别为0.93±0.45、0.69±0.12,两组CRH mRNA表达量比较,P〈0.05。结论侧脑室注入omentin对小鼠摄食量及下丘脑组织NPY、AGRP、POMC mRNA表达无影响,但CRH mRNA表达增加。 [ABSTRACT FROM AUTHOR]

Objective To investigate the identification of octreotide (OCT) modified chitosan (CS) miR-155 molecular beacon nanoparticles (CS-miR-155-MB-OCT) and imaging of lung cancer cells for the early screening of lung cancer. Methods A nude mouse model of lung transplantation tumor was established by injecting A549 lung cancer cells into tail veins to establish lung xenograft models. Cre adenovirus was injected through nasal cavity, and mice were killed at 4, 6, 8 and 12 weeks after adenovirus injection to establish lung cancer models of atypical hyperplasia, adenoma, carcinoma in situ and adenocarcinoma of lung in LSL K-ras G12D transgenic mice at different pathological stages. Lung tissue samples were taken and observed by HE staining. Immunohistochemistry were used to detect the expression of somatostatin receptor 2 (SSTR2). Real-time fluorescence quantitative PCR was used to detect miR-155 expression levels in lung xenograft models and transgenic mice at different stages of lung cancer. Then CS-miR-155-MB and CS-miR-155-MB-OCT were injected via tail vein in lung xenograft models. CS-miR-155-MB-OCT was injected via tail vein in transgenic mice models. The fluorescence signals of lung in nude mice and transgenic mice at different disease stages were imaged by living imaging system. Frozen slices of lung tissue were made. The source of fluorescence signal was detected by laser confocal scanning microscope (CLSM). Results HE staining showed that lung transplantation tumor models and lung cancer models of atypical hyperplasia, adenoma, carcinoma in situ and lung adenocarcinoma at different pathological stages were successfully constructed. Immunohistochemical analysis showed somatostatin receptor 2 (SSTR2) was expressed in transplanted lung tumor and tissue at different pathological stages. In transgenic mouse models，the expression of miR-155 was gradually increased as the disease progressed (P＜0.05). In lung xenograft models, the fluorescence signals were significantly higher in the CS-miR-155-MB-OCT group than those of the CS-miR-155-MB group (P＜0.05). In transgenic mouse models, the fluorescence signals gradually increased with the gradual progression of lesions (P＜0.05). After re-imaging the lung tissue, it was found that the fluorescence signal came from lung, and CLSM showed that the fluorescence signal came from cancer cells and some normal alveolar epithelial cells. Conclusion CS-miR-155-MB-OCT can dynamically reflect the occurrence and development of lung cancer according to changes of different fluorescence intensity, thus providing a new technology for the early diagnosis of lung cancer. [ABSTRACT FROM AUTHOR]

[Objective] The present experiment was conducted to investigate ecologieal characteristics of strain BJM-11 and the purpose is to provide scientific basis for fermentation production and field application. [Method ] The temperature, pH ,carbon sources,nitrogen sources and other factors of BJM-11 were determined by measuring the diameter of the colony and counting the number of conidia using hemocytometer measurement. [ResultJThe temperature range for the growth and sporulation of strain BJM-11 was 10 -35°C .including colony diameter at 25 - 30 °C temperatures significantly greater than other treatments! P <0. 05 , the same as below). The optimal temperature for sporulation of BJM-11 was 25 CV. which was significantly greater than other treatments. The pH value range for the growth and sporulation of strain BJM-11 was 4. 0 - 12. 0°C .including colony diameter at pH value 5.0 -6. 0 significantly greater than other treatments. The optimal pH value for sporulation was 5.0, 6.0 and 7.0 which was significantly greater than other treatment. Strain BJM-11 could grow and sporulate under the condition of continuous light for 24 hours, continuous darkness for 24 hours and alternating light and dark, of which colony diameter of continuous darkness for 24 hours was significantly greater than other treatments. The optimal light condition for sporulation was continuous light for 24 hours which was significantly greater than other treatments. Strain BJM-11 could grow and sporulate under eight carbon source culture media of which colony diameter of glucose and soluble starch was larger and optimal carbon source for sporula-tion was mannitol which was significantly greater than other treatment. Strain BJM-11 could grow and sporulate under eleven nitrogen source culture media of which colony diameter of yeast powder and peptone was significantly greater than other treatments. The optimal nitrogen souree for sporulation was peptone which was significantly greater than other treatments. Strain BJM-11 could glow and sporulate on the five kinds of media,in which colony diameter on PDA and PSA were significantly greater than other treatments. The optimal medium for sporulation was PDA which was significantly greater than other treatment. [Conclusion] Mycelia growth of strain BJM-11 was better under the condition of 25 -30 temperature,pH value 5.0 - 6. 0,the continuous darkness,glucose or soluble starch as carbon source, peptone or yeast powder as nitrogen source and medium for PDA or PSA. The spore production quantity was larger under the condition of 25 -30 temperature, pH value 5.0 -7.0, the continuous light, mannitol as carbon source, peptone as nitrogen source,culture medium for PDA. [ABSTRACT FROM AUTHOR]

Nonalcoholic fatty liver disease (NAFLD) is a group of highly heterogeneous diseases closely associated with metabolic dysfunction. Sarcopenia is a syndrome caused by a continuous decline in muscle mass, strength, and function, and it is often accompanied by NAFLD. Insulin resistance is the main pathological mechanism for sarcopenia and NAFLD, and in addition, factors such as changes in proteins and branched-chain amino acid, hyperammonemia, intestinal flora, and endocrine dysfunction can also lead to sarcopenia and NAFLD. With the deepening of clinical research, many published prospective studies have confirmed the existence of a bidirectional and complex pathophysiological relationship between sarcopenia and NAFLD. This article reviews the bidirectional relationship between sarcopenia and NAFLD, discusses the common pathogenesis of sarcopenia and NAFLD, summarizes the challenges faced in this field, and proposes new directions for the research on the bidirectional relationship between NAFLD and sarcopenia. [ABSTRACT FROM AUTHOR]

In this article, the morphological characteristics of indium droplets with different deposition amounts on GaAs ( 001 ) substrates at low temperature are studied. Under the condition of a substrate temperature of 160 °C, the morphology of indium droplets with different deposition amounts is analyzed, and the formation mechanism of indium droplets with different deposition amounts is explained based on the classical nucleation theory. Through the statistics and analysis of the number, diameter and height of the droplets and the diameter and height of the expanding disk around the droplets, the evolution of the droplet morphology with the deposition amount is explained. According to the related theory of droplet morphology and deposition amount and the data obtained in the experiment, the minimum deposition amount of indium droplet that can produce diffusion disc is about 3. 3 ML. Related research results have guiding significance for the preparation of InAs nanostructures by droplet epitaxy. [ABSTRACT FROM AUTHOR]

Pyrazine compounds are widely used in pharmaceutical, medicine, chemistry and other fields due to their unique structure and activity. Using water as solvent, hydrothermal method is the preferred method for complex synthesis because of its green, pollution-free and simple operate. In this paper, 2,6-bis (2-pyrazinyl) pyridine-4-p-benzoic acid ( Hbppc) and CdCl2·2. 5H2O were used as raw materials, a new coordination compound [ Cd ( bppc) (H2O) Cl] n was synthesized by hydrothermal method, and its structure was characterized by single crystal X-ray diffraction, infrared spectroscopy and Raman spectroscopy. The results show that the complex has a one-dimensional chain structure. The fluorescence analysis of free ligand 2,6-bis (2-pyrazinyl) pyridine-4-p-benzoic acid and its complex [ Cd ( bppc) (H2O) Cl] n show that the obtained complex has good fluorescence property. [ABSTRACT FROM AUTHOR]

Droplet epitaxy technology is not only suitable for lattice mismatch, but also suitable for lattice matched material systems, and is easy to prepare low - dimensional semiconductor structures, such as low-density quantum dots, rings, etc. In this paper, the growth of quantum dots with different Al and Ga compositions on GaAs surface by droplet epitaxy method was studied. In the experiment, the samples were monitored in situ with Reflection High Energy Electron Diffraction (RHEED). The composition of Al and Ga droplets deposited on the substrate was controlled by controlling the deposition rate of Al and Ga droplets. It was found that the quantum dots become denser and the wetting angle became lower with the increase of Al composition. When the Al component increased by more than 0. 5, quantum dots of different sizes appeared, and the dot density increased exponentially. An empirical formula is given and the phenomenon is explained. [ABSTRACT FROM AUTHOR]

Cadmium complexes are widely used in the fields of optics, medicine, chemistry and so on because of their unique structure and properties. Hydrothermal method is one of the most convenient and effective methods to synthesize complex at present because of its solvent cleaning and simple operation. A compound [ Cd2 ( L)2 ( bdc) ( H2 O)4 ] ( bdc) was hydrothermally synthesized using 2,6-di(pyrazin-2-yl)-4,4′-bipyridine(L), terephthalic acid and CdCl2 ·2. 5H2 O as raw materials, the structure and luminescent properties of the complexes were characterized by single crystal X-ray diffraction and infrared spectroscopy. The experimental results show that the [Cd2 (L)2 (bdc) (H2 O)4] (bdc) belongs to the triclinic system, P1 space group, a =1. 002 78(17) nm, b = 1. 044 25(17) nm, c = 1. 274 8(2) nm, β = 78. 559(2)°, and has good fluorescence. [ABSTRACT FROM AUTHOR]

BACKGROUND: When using inductively coupled plasma - mass spectrometry ( ICP - MS) to analyze soil, the zirconium hydroxide, molybdenum oxide, and tin isotopes can cause serious interference on cadmium, which makes it difficult to accurately determine cadmium in soil samples. OBJECTIVES: To develop a method for accurate determination of Cd in soil samples. METHODS: ICP - MS/MS was used, and m Cd without isobaric interference was selected as the analysis line. In MS/MS mode, the oxygen introduced into the collision reaction cell can be reacted with m Zr16 0 1 H +, m Mo 16 0 +, inducing electron transfer, dehydrogenation, oxygenation and other reactions to inhibit the overlapping interference of these polyatomic ions on m Cd. RESULTS: Oxygen flow was the key factor in this method. 0. 45mL/min oxygen flow can reduce oxide interference to less than 0. 005 μg/L when zirconium concentration was lower than 2000 μg/L and molybdenum concentration was below IOOOμg/L. The method has been verified by the national reference materials. The relative error of the measured value and recognized value of the 11 national reference materials was within ± 7%, and the relative standard deviation ( n = 12) of three soil reference materials was less than 5%. CONCLUSIONS: Triple quadrupole ICP - MS oxygen reaction mode can eliminate Zr and Mo polyatomic ion interference on Cd, and this method can be used for the determination of trace Cd in soil samples. [ABSTRACT FROM AUTHOR]

Objective To observe clinical efficacy of electroencephalograph bionic electrical stimulation(EBES) combined with rehabilitative treatment in patients with sympathetic cervical spondylosis (SCS) . Methods From Jan. 2018 to Jun. 2019,46 patients with SCS, admitted in the First Affiliated Hospital of Anhui Medical University, were enrolled and randomly assigned into observation group and control group,with 23 cases of each group. Both groups were given routine rehabilitation treatment, including cervical traction, massage, exercise therapy and so on. The observation group was also treated with EBES. Clinical assessment scale for cervical spondylosis(CASCS) was used to evaluate the clinical efficacy before and after treatment. Meanwhile,1 year after treatment, the recurrence rate of the two groups was investigated to evaluate t he long term efficacy. Results After treatment,the scores of CASCS,the total effective rate and excellent rate in observation group was better than control group(P<0. 05). The long term recurrence rate in observation group was lower than control group (P<0. 05). Conclusion EBES combined with rehabilitation treatment could improve the clinical efficacy and reduce the long term recurrence rate of patients with SCS. [ABSTRACT FROM AUTHOR]