Search

【Objective】Deqing County, Guangdong Province is a native habitat of Morinda officinalis How., a genuine medicinal herb in China. In the study, the genetic diversity of germplasm resources of 24 varieties of Morinda officinalis How. were analyzed.【Method】A total of 24 Morinda officinalis How. resources collected from Deqing County as research objects, and principal component analysis and molecular marker methods (SRAP and CDD) were used to analyze the genetic diversity of 24 Morinda officinalis How. materials.【Result】Phenotypic analysis showed that, in the cluster map based on principal component analysis, Guanwei Town, Gaoliang Town, Fengcun Town and Gaoliang Town were clustered in four groups. In addition,100 and 98 total bands were obtained in 18 pairs of SRAP primer combinations and 16 CDDP primers, among them, there were 50 SRAP polymorphic bands, resulting in a polymorphism ratio of 50%. The average genetic similarity coefficient (GS) was 0.87, and the GS ranged from 0.71 to 0.97. There were 80 polymorphic bands in CDDP, with a polymorphism ratio of 79.0%. The GS among germplasm resources ranged from 0.71 to 0.96, with an average (GS) of 0.86. When the coefficient was 0.82, both SRAP and CDDP molecular markers showed certain similarities among the germplasm resources of Gaoliang Town, Guanxu Town and Fengcun Town.【Conclusion】Combined with the clustering results of phenotypic and molecular marker, the genetic relationship is closest between Gaoliang Town and Guanwei Town as well as Gaoliang Town and Fengcun Town. The results of SRAP and CDDP molecular marker methods have certain differences in the genetic diversity of Morinda officinalis How., and the combined analysis can better reflect the genetic diversity of Morinda officinalis How. and the phylogenetic relationship of Morinda offi cinalis How. resources. [ABSTRACT FROM AUTHOR]

Objective Exploring the efficacy and safety of botulinum toxin type A （BTX-A） combined with pulsed radiofrequency（PRF） in the treatment of postherpetic neuralgia（PHN）. Methods A total of 80 patients with PHN were collected. They were randomly divided into experimental group （Group B）and control group （Group C）, Group B was treated with BTX-A intradermal injection combined with PRF, and Group C was treated with lidocaine intradermal injection combined with PRF. Numeric pain score （NRS）, Simplified McGill Pain Questionnaire （SF-MPQ）and Sleep Quality Score （QS）were used to assess the patients' pain level and sleep quality preoperatively, 1, 3, and 7 days postoperatively, and 1, 2, and 3 months postoperatively. The patients' postoperative adverse reactions were collected. Interleukin-1β （IL-1β）and calcitonin gene-related peptide （CGRP）levels in patients' serum were measured preoperatively and 3 days postoperatively. Results The NRS scores, SF-MPQ scores, and QS scores of group B and group C were significantly lower at all postoperative time points compared to preoperative ones （P < 0.05）. The NRS and SF-MPQ score were significantly lower in group B at 1, 2, and 3 months postoperatively compared with group C（P < 0.05）; and group B had significantly lower QS scores at 2 and 3 months postoperatively （P < 0.05）. The effective rate of pain relief at 3 months postoperatively in group B （90%） was statistically significant （P < 0.05） compared with group C （56.7%）. No serious adverse reactions occurred in either group. The levels of IL-1β and CGRP in serum of patients in both groups were significantly decreased at 3 days after surgery compared with the preoperative period, and the degree of decrease of IL-1β and CGRP in group B was more significant than that in group C （P < 0.05）. Conclusion BTX-A combined with PRF treatment for PHN can effectively reduce its pain level, improve the quality of sleep, and is safe. [ABSTRACT FROM AUTHOR]

[Objective] The aims of this study are to investigate the dynamics of vegetation coverage changes and their underlying drivers in the northwestern Sichuan Plateau region, and to supply a theoretical foundation for regional vegetation conservation and restoration initiatives. [Methods] MODIS NDVI was utilized as the foundational dataset. By integrating Sen′s trend analysis, Manna-Kendall test, and the Hurst model, we comprehensively analyzed the spatial distribution characteristics, trends spanning nearly 20 years, and prospective sustainable state of the region. Moreover, the study employed geographical detectors to delve into the underlying driving forces. [Results](1) The overall vegetation cover in the area was relatively great in the east.(2) In the whole period, the vegetation cover of 80.56% of the regions showed no significant change, and the vegetation cover of the regions with significant improvement accounted for 17.80%.(3) The Hurst index indicates that the overall vegetation cover will be in the development state of no significant change in the future.(4) The one-factor explanatory power of elevation, annual average temperature and soil type surpassed more than 30%. They were the main factors driving the formation of differences in vegetation cover distribution patterns. The interaction and synergy of each factor were enhanced. There were obvious differences in the intervals or types of suitable vegetation growth of each factor.(5) The natural factors dominated the formation of the overall pattern of spatial distribution of regional vegetation cover, whose land use types were the key factors in driving its dynamic change. In the past 20 years, the vegetation cover in the West Sichuan Plateau had changed significantly, and natural factors had dominated the spatial distribution pattern of regional vegetation cover. [Conclusion] In the past 20 years, the vegetation cover in the study area had a clear change pattern. Although natural factors dominate the spatial layout of vegetation cover, human activities are the main driving factors for its pattern change. [ABSTRACT FROM AUTHOR]

The influence of strand saddle tube's diameter on the stress condition of each member in the pylon anchorage zone was analyzed based on Wangjia River Bridge and field test. A strong-weak zone model of pylon anchorage component with strand-separating saddle was proposed. The finite element models of saddle anchorage and crossing anchorage were established, and the stress of the components of the two anchorages were compared. The results show that the cable stresses of saddle anchorage are nearly 51. 4% of those in the cross-anchorage under the same conditions, and the stress conditions of saddle anchorage are better than those of the latter overall. The force transmission paths and optimized geometric configuration of saddle anchorage zone, and the generalized strong-weak zoning model of concrete around the ends of strand-separation tube are obtained by using the topology optimization method. The upside length of the generalized model is approximately equal to 0. 55 times outer diameter of strand-separation tube, and the failure angle of concrete is about 56°. Finally, the concrete strength of the critical section are checked. The strong-weak model could provide a theoretical reference to the similar structural design. [ABSTRACT FROM AUTHOR]

At present, the standard still has certain limitations on the use of machine-made sand (MMS). It does not consider the impact of different lithology and grain types on the particle gradation. The performance of MMS concrete in actual production is not satisfactory. The effect of particle size on the fineness modulus (FM) guides the production of MMS. By reducing the range of value of the MMS grade required by the specification, the granite MMS concrete bleeding condition is improved. At the same time, a sand rate correction method considering the FM, micro fines content, and water-cement ratio is proposed, which is a new attempt for the use of granite MMS concrete and accumulates experience for the subsequent engineering application of granite MMS concrete. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects and mechanism of microRNA ( miR-130a) on proliferation and apoptosis of pancreatic cancer cells by targeting FOS-like antigen 1 (FOSLl). Methods: Pancreatic cancer cells PANC-1 were routinely cultured and divided into the NC group and miR-130a mimic group. Cells in the NC group were transfected with control, miR-130a mimic group with miR-130a simulant by Liposome 2000. CCK-8 was used to detect the proliferation activity of cells. Flow cytometry was used to detect the apoptosis of cells. Targetscan7. 1 prediction software and dual luciferase reporter gene test were used to detect miR-130a targeted-regulating FOSLl gene. The FOSLl overexpression plasmid was constructed, and co-transfected into PANC-1 cells with miR-130a, and then PANC-1 cells were divided into the NC group, miR-130a mimic group, and miR-130a mimic + oe-FOSLl group. Cells in the NC group were transfected with control, miR-130a mimic group with miR-130a mimic, and the miR-130a mimic + oe-FOSLl group with miR-130a mimic and FOSLl overexpression plasmid. Western blotting was used to detect the expression of p53 protein in each group. Results: Compared with the NC group, the proliferation ab山ty of PANC-1 cells in the miR-130a mimic group decreased (P < 0. 05), and apoptosis increased (P < 0. 05). The double luciferase reporter gene test coufirmed that FOSLl was a target gene of miR-130a. Compared with the NC group, the proliferation ability of PANC-1 cells in miR-130a mimic group and the miR-130a mimic + oe-FOSLl group decreased, and apoptosis increased (both P < 0. 05). Compared with the miR 130a mimic group, the proliferation ability of PANC-1 cells in the miR-130a mimic + oe-FOSLl group increased, and apoptosis decreased (both P < 0. 05). Compared with the NC group, the expression of FOSLl protein in the miR-130a mimic group and miR-130a mimic + oe-FOSLl group decreased and p53 protein increased ( both P < 0. 05 ). Compared with the miR-130a mimic group, the expression of FOSLl protein in the miR-130a mimic + oe-FOSLl group increased and p53 protein expression decreased ( both P < 0. 05 ). Conclusion: MiR-130a targeting FOSLl can inhibit the proliferation of pancreatic cancer cells and promote apoptosis by up-regulating the expression of p53. [ABSTRACT FROM AUTHOR]

Objective To verify the value of whole genomic copy number variation (WGCNV) detection and scoring system in the diagnosis and prognosis of lung adenocarcinoma. Methods Seventy-six lung adenocarcinoma specimens including ninety-one tumor samples and twenty adjacent non-tumor lung tissue samples were collected using Laser capture microdissection (LCM). Whole genomic amplification (WGA) was used to enrich DNA and construct a sequencing library for next generation sequencing (NGS). Changes of larger than 5Mb CNV in this study were analyzed and scored. The nuclear grading and score of tumor cells in the surgery and pleural effusion cytology of lung adenocarcinoma specimens were evaluated separately. For each case, we evaluated (1) nuclear size, (2) mitotic counts, (3) nuclear atypia, (4) atypical mitoses. The data of disease-free survive (DFS) and overall survive (OS) were collected for assessing the prognostic value of WGCNV score. Meanwhile, receiver operating characteristic (ROC) and area under curve (AUC) were used to define a cut-off value and evaluate the diagnostic significance in lung adenocarcinoma. Results The WGCNV scores of twenty adjacent non-tumor lung tissue samples were treated as normal control and all of WGCNV scores of tumor samples range from 0 to 9.95, the median score was 2.7. The WGCNV scores were divided into three groups: low score group <1.74, medium score grade 1.74~4.23, high score grade >4.23. The WGCNV score was positively associated with the nuclear grade scoring (r=0.780 90, P<0.001). The result for evaluation of prognostic value of the WGCNV scores showed that comparing with low WGCNV score group, Hazard Ratio (HR) of medium score group was 4.11 (95%CI=0.72~23.57) and high score group was 2.07 (95%CI=0.30~14.12). These results suggested that the risks of the medium and high WGCNV score group elevated. According to the analysis results of ROC curve, when the cut off value was 0.01, the sensitivity and specificity for lung adenocarcinoma diagnosis were 97.8% and 95.0% respectively, the positive predictive value (PPV) and negative predictive value (NPV) were 99.0% and 90.1%, respectively, the AUC was 0.981. In the differentiation of adenocarcinoma in situ (AIS) and minimally invasive adenocarcinoma (MIA) group and invasive adenocarcinoma group, when the cut off value was 1.8, the sensitivity and specificity between the two groups were 78.1% and 94.4%, and the PPV and NPV were 98.0% and 52.0%, respectively, the AUC was 0.896. Conclusion This study verifies that WGCNV scoring system has a potential diagnostic and prognostic value in lung adenocarcinoma. [ABSTRACT FROM AUTHOR]

PHengLEI is a software platform of Computational Fluid Dynamics (CFD) developed by China Aerodynamics Research and Development Center (CARDC) with completely independent intellectual property rights. It has been widely used in China. As an important part of the national numerical wind tunnel system, PHengLEI are integrating the discipline physical models including helicopter rotor, aircraft icing, wind engineering, chemical reaction and combustion, virtual flight, high order method on both structured and unstructured grids. In order to meet the needs of integrated development of multidisciplinary and multi-physical models and to adapt to the next generation of high-performance computer hardware, based on the existing work, the architecture and data structure of the next-generation PHengLEI software are further designed. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of leonurine( LEO) on the cerebral ischemia-reperfusion injury mice and its mechanism. Methods Totally 120 healthy male Kunming mice were randomly divided into three groups: the sham group,the model group,and the LEO group,with 40 mice in each group. The cerebral ischemia reperfusion models were established by the middle cerebral artery occlusion method in mice of all groups except the sham group. The mice in the sham operation group were operated in the same method except that the suture was not inserted. At 30 minutes after model establishment,the mice in the LEO group was intraperitoneally injected with LEO 15 mg/( kg·d). The mice in the sham group and the model group received the same volume of normal saline. At 24 h after model establishment,the brain tissues of the infarction site were taken and the volume of cerebral infarction was measured by TTC staining. The brain water content was weighed to evaluate the degree of brain edema. The blood-brain barrier permeability was measured by Evans blue( EB) method. The morphology of brain tissue was observed by HE staining. The expression of p-p38 and tight junction protein( ZO-1) protein in the brain tissues was detected by Western blotting. The expression of inflammatory factors including IL-1β,IL-6 and tumor necrosis factor-α( TNF-α) mRNA was detected by RT-PCR. Results The cerebral infarction volume ratio,brain water content,and EB content in the model group were higher than those in the sham operation group and were lower than those in the model group( all P < 0. 05). In the model group,a large number of neurons showed pathological changes of ischemic anoxic necrosis. However,compared with the model group,the pathological changes of brain tissues in the LEO group were significantly improved. The expression of p-p38 protein was higher and the expression of ZO-1 protein was lower in the model group than that in the sham group. The expression of p-p38 protein was lower and the expression of ZO-1 protein was higher in the LEO group than that in the model group( P < 0. 05). The expression levels of IL-1β,IL-6,and TNF-α mRNA were higher in the model group than those in the sham group. The expression levels of IL-1β,IL-6 and TNF-α mRNA were lower in the LEO group than those in the model group( all P < 0. 05). Conclusion LEO can improve the nerve damage and alleviate the permeability of blood-brain barrier in mice with ischemia-reperfusion.The mechanism may be related to the inhibition of p38 MAPK signaling pathway and reduction of the inflammatory response. [ABSTRACT FROM AUTHOR]

Based on the teaching contents of unmanned aerial vehicle ( UAV) High t control and image processing in unmanned aerial vehicle remote sensing course, the paper designed and built a four rotor UAV teaching experimental platform based on photoelectric image processing. In order to achieve the autonomous Bight control of the quadrotor, an autonomous flight control hardware platform is designed in which a self-assembly aircraft is used as the body frame based on image processing and recognition. The main on-board sensor is attitude sensor MPU6050 and camera OV7725. An ARM microcontroller is used to construct the main control chip, R5F100LEA series chip is used as assistant chip. Based on the nonlinear dynamic model of the quadrotor, a single-loop P1D control algorithm and image processing and recognition are designed, this design realizes the self-assembly aircraft system which can fulfill specific task. Experimental results show that the on-board control system has achieved good flight performance and we finish the following task like following the line, picking and searching specific things with the corporation of two chips. [ABSTRACT FROM AUTHOR]

In previous studies, only analysis was conducted on the structure and internal flow field characteristics of the control valve labyrinth channel, but the optimization design of the anti-cavitation performance and flow performance of the labyrinth channel was relatively lacking. In order to meet the design requirements of the valve in practical engineering, the labyrinth type regulating valve needs to have channel anti-cavitation performance and flow performance. Therefore, a method based on multi-objective genetic algorithm (MOGA) and back propagation neural network(BPNN) was proposed to optimize the structure of the regulating valve labyrinth flow channel, and improve its anti-cavitation performance and flow performance. Firstly, based on the principle of hedging energy dissipation and the principle of multi-stage bucking, the arc-shaped hedging maze flow channel was designed, and a mathematical model for fluid dynamics simulation calculation was established. Then, the model was simulated using computational fluid dynamics(CFD) simulation software. And based on the simulated data, the BPNN surrogate model was constructed. The Sobol sensitivity analysis method was combined with the surrogate model. The influence of the parameters of the maze flow channel on the simulation results was analyzed. The structure of the flow channel was optimized by multi-objective genetic algorithm. Finally, a test platform was built to measure the blocking flow curve of the flow channel. The results show that the maximum flow rate of the maze flow channel obtained by the optimization algorithm is increased from 0.087 6 kg/s to 0.117 4 kg/s, which is increased by 34%. And the linear pressure difference is increased from 762.163 kPa to 811.280 kPa, which is increased by 6%. The actual maximum flow rate of the optimized maze flow channel is 0.115 9 kg/s, which is increased by 33%, and the linear differential pressure is 819 kPa, which is increased by 7%. The cavitation resistance and flow performance of the maze runner are improved at the same time, which proves the effectiveness of the simulation and the feasibility of the proposed method. [ABSTRACT FROM AUTHOR]

In order to solve the problem of weak covertness, small hiding capacity and poor robustness of Tibetan webpage information hiding (111) technique, a novel 1H scheme with synchronization mechanism for Tibetan web page ( 1HSTW ) is proposed. Firstly, the bit sequence of secret message is disordered by scrambling transformation algorithm. Then the synchronous Baker code is added into this scrambled bit sequence to form a new bit sequence. Lastly, the bit "0" and ' 1' of this new bit sequence is embedded into Tibetan webpage through changing the upper or lower case of tag attribute names in its HTML code. Experimental results show that, compared with other existing III methods, I11STW can increase the information hiding capacity effectively with good covertness and present better robustness under heavy random perturbation. [ABSTRACT FROM AUTHOR]

Objective To investigate the changes in levels of portal venous blood prostacyclin I2 ( PGI2) and thromboxane A2 (TXA2) in patients with colorectal carcinoma and their clinical significance. Methods Sixty patients with colorectal carcinoma were selected in the study，including 22 cases with synchronous liver metastasis ( liver metastasis group )，38 cases without liver metastasis ( non-liver metastasis group )，20 cases in stage II of pTNM staging，32 cases in stage H ，and 8 cases in stage IV. Meanwhile，16 cases of postoperative adhesive intestinal obstruction were considered as controls ( control group ). The levels of PGI2 and TXA2 in the portal venous blood，tumor tissues，and normal colorectal mucosae were detected by radioimmunoassay，respectively ，and then were compared with those of the controls. Results Compared with the normal colorectal mucosae，the level of TXA2 increased，while the level of PGI2 decreased significantly in the colorectal cancer tissues ( both P < 0 .01 ); compared with the control group，the level of PGI2 increased，and the ratio of PGI2/TXA2 and TXA2 decreased significantly in the non-liver metastasis group and liver metastasis group ( all P < 0. 01 ). Compared with the non-liver metastasis group， the level of portal venous blood PGI2 and the ratio of PGI2/TXA2 decreased，TXA2 increased in the liver metastasis group (all P < 0.01). Compared with patients whose tumor maximum diameter ≤3.0 cm，3. 1 cm - 4 . 0 cm，the level of PGI2 and the ratio of PGI2/TXA2 decreased，TXA2 increased in patients with tumor diameter > 4 .0 cm ( all P < 0.05 ). The ratio of PGI2/TXA2 value of portal vein was negatively correlated with the size of the tumor ( r = -0 .770 ). The ratio of PGI2/TXA2 in portal vein blood of patients with colorectal cancer and synchronous liver metastasis was lower than that without metastasis ( P < 0 .01 ). Conclusions The level of TXA2 increases and the ratio of PGI2/TXA2 decreases in the portal vein blood of patients with colorectal cancer. The decreased ratio of PGI2/TXA2 of portal vein blood may be one of the important causes of synchronous liver metastasis of colorectal cancer. [ABSTRACT FROM AUTHOR]

In this paper, molecular dynamics (MD) method was used to simulate the binding energy, cohesive energy density, radial distribution function and mechanical properties of the polymer bonded explosives (PBXs) systems constructed by polyurethane (Estane 5703), Ethylene - Propylene - DieneMonomer (EPDM), fluoropolymer (F2311) and 2, 6 - dipicrylamino - 3, 5 - dinitropyridine (PYX) (011 ) crystal face. The results showed that the interaction between Estane 5703 and PYX (011) is the strongest. The order of cohesive energy density between different binders and crystals is PYX/F2311 > PYX/Estane 5703 > PYX/EPDM. The radial distribution function analysis showed that the interaction between PYX (011 ) crystal face and polymer molecules is mainly electrostatic interaction. After adding three kinds of binders, the tensile strength and breaking strength of the PBX system are improved. In addition to F2311, the addition of two other binders improves the shear strain resistance of PBX system. [ABSTRACT FROM AUTHOR]

The molecular structures, Mulliken charge populations, molecular electrostatic potentials (MEP) and Hirshfeld surfaces of 2,2’,4,4’,6,6’-hexanitrobiphenyl (HNBP), 2,2’,4,4’,6,6’-hexanitrostilbene (HNS), 2,5-dipicryl-1,3,4-oxadiazole (DPO) and 5,5’-bis (2,4,6-trinitrophenyl) -2,2’-bis (1,3,4-oxadiazole) (TKX-55) were simulated and calculated with the first principles method. By studying the molecular properties, electronic properties and intermolecular interactions, we can understand the heat resistance mechanism of energetic compounds with high heat resistance. The results showed that the complexity of the bridge connection structure and the strong intermolecular hydrogen bond interaction can enhance the stability of energetic compounds. In addition, it was found that the addition of intermediate groups would affect the charge distribution of carbon atoms on aromatic rings on both sides of the molecules and the area of positive and negative electrostatic potential regions on the surfaces of the four energetic compounds. [ABSTRACT FROM AUTHOR]

Peanut southern blight is a fungal disease which is caused by the pathgen Sclerotium rolfsii Sacc., and seriously restricts the quality and yield of peanut. In this study, 116 strains of actinomycetes were isolated and purified from rhizosphere soil of Achyranthes bidentata by dilution coating method, and the strains that can control peanut southern blight were screened and identified. Two strains, Soil-1-5 and Soil-3-28, with good and stable antifungal activity, were screened by the plate confrontation test, and their inhibition rates were 80.43% and 92.34%, respectively. Morphological observation, physiological and biochemical tests, and 16S rRNA gene sequence analysis identified strains Soil-1-5 and Soil-3-28 as Streptomyces scabiei and Streptomyces luteogriseus, respectively. The inhibition of aseptic fermentation filtrate of antagonistic strains on plant pathogens was measured by mycelium growth rate method. The results showed that after 5 times dilution of aseptic fermentation filtrate of strains Soil-1-5 and Soil-3-28, the inhibit rates of S. rolfsii were 73.67% and 57.11%, respectively, and the two strains had different degrees of inhibition effects on the six plant pathogens including Fusarium graminearum. In addition, the two strains of aseptic fermentation filtrate also had good antagonistic effects on the sclerotinia germination and sclerotinia formation. The results of pot experiment showed that the control effects of strain Soil-1-5 and Soil-3-28 on peanut southern blight were 51.92% and 31.74%, respectively, and strain Soil-3-28 could promote the growth of peanut. In conclusion, strains Soil-1-5 and Soil-3-28 had better control efficacy on peanut southern blight, and had potential application value. [ABSTRACT FROM AUTHOR]

Bladder cancer is the most common malignant tumor in the urinary system. Currently, the clinical treatment options for bladder cancer mainly include surgery, chemotherapy, radiotherapy, immunotherapy, targeted therapy, photodynamic therapy, combination therapy, etc. The conventional treatment and administration strategies for bladder cancer primarily depend on the tumor stage and the extent of metastasis. However, in the process of non-surgical treatment, drugs lack specificity and targeting. Once the dosage is improperly controlled, drugs will damage normal cells when attacking cancer cells, which will lead to poor efficacy and multiple side effects. Nanomedicine is an emerging interdisciplinary field that utilizes nanomaterials and technologies in nanomedicine to provide disruptive technologies for traditional treatments, with advantages such as targeted delivery and high efficiency with low toxicity. Many nanotechnologies have become hot topics in clinical research in the field of medicine. Functionalized nanoparticles can actively or passively target specific cells within target organs, such as bladder cancer cells, by altering their surface properties, thereby enhancing drug delivery precision, reducing damage to normal cells, and improving treatment efficacy. This article provides an overview of the progress in classical and novel treatment approaches to bladder cancer, with a particular focus on the potential applications and future development directions of nanotechnology in the treatment of bladder cancer, providing important reference for personalized therapy and clinical translation in bladder cancer. [ABSTRACT FROM AUTHOR]

Objective: To investigate the factors that affect the success rate, infarct volume and behavior score stability of cerebral ischemia-reperfusion model in mice. Methods: 75 Kunming mice, weighing 20-23 g, were randomly divided into 5 groups. The effects of different sizes of wire bolts and depth of insertion on the success rate, the volume of infarct and the stability of behavior score in the model of cerebral ischemia-reperfusion in mice were compared. At the same time, the intraoperative cerebral blood flow monitoring was performed to compare the differences of cerebral blood flow in the middle cerebral artery of each group. Results: Specifications 1 group, the success rate of the model was 40%, the infarct volume percentage was (16.7± 9.3)%, the neurological deficit score (NSS): 7.2± 2.4, the percentage of middle cerebral artery blood flow decreased (86.9± 4.2)%; specifications 2 group, the success rate of the model was 46.7%, the percentage of infarct volume was (19.2± 11.6)%, NSS:8.8± 2.5, the percentage of MCA blood flow decreased: (87.4± 3.8)%; specifications 3 group, the success rate of the model was 33.3%, the percentage of infarct volume was (16.6± 9.6)%, NSS:8.2 + 2.6, the percentage of MCA blood flow decreased: (88.3 + 3.4)%; specifications 4 group, the success rate of the model was 86.7%, the percentage of infarct volume was (23.4 ± 2.2)%, NSS:13.9 ± 1.3, the percentage of MCA blood flow decreased: (87.5 ± 3.5)%; specifications. Conclusion: 1. The key factor of the stability of the cerebral ischemia reperfusion model in mice is that the suture can effectively plug the posterior communicating arteryand the anterior segment of the anterior cerebral artery. 2. The monitoring of middle cerebral artery flow could not be used as the basis for evaluating the success of cerebral ischemia reperfusion model in mice. [ABSTRACT FROM AUTHOR]

The traditional medical text data classification methods ignore the context of the text. Each word is independent of each other and cannot represent semantic information. The text description and classification effect are poor, and feature engineering requires manual intervention, so the generalization ability is not strong. Aiming at the problems of low efficiency and low accuracy of medical text data classification, this paper proposes a medical text classification model CMNN based on bidirectional encoder representations from Transformer (BERT), convolutional neural network (CNN) and Bi-directional long and short-term memory(BiLSTM) neural network. The model uses BERT to train word vectors and combines CNN and BiLSTM to capture local latent features and contextual information. Finally, the proposed model is compared with the traditional deep learning models TextCNN and TextRNN in terms of accuracy, precision, recall and F1 score. The experimental results show that the CMNN model outperforms other models on all evaluation metrics, and the accuracy is improved by 1.69%～5.91%. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of T-cadherin combined with cisplatin on cisplatin resistant malignant melanoma cell line. Methods CDDP resistance B16F10 (CDDP-R B16F10) was induced by using high and gradually increased dose of CDDP．MTT assay was used to test the proliferation of CDDP-R B16F10. The T-cadherin was transfected into CDDP-R B16F10 cells. The expressions of T-cadherin mRNA and protein were measured by reverse transcription polymerase chain reaction (RT-PCR) and SP immunohistochemistry method. There were six groups in this study including control group, pEGFP-N1 group, pEGFP-N1-T-cadherin group, cisplatin group, pEGFP-N1 combined with cisplatin group and pEGFP-N1-T-cadherin combined with cisplatin group. The effects of T-cadherin combined with cisplatin on migration and invasion of CDDP-R B16F10 were determined by Wound-healing assay and Transwell invasion assay. Factor analysis method was used to evaluate the interactions of T-cadherin and CDDP on migration and invasion of CDDP-R B16F10. Results The CDDP-R B16F10 cell line was successfully established. There was no statistical difference in proliferation between CDDP-R B16F10 cells and B16F10 cells (P＞0.05). RT-PCR and SP immunohistochemistry assay showed that Tcadherin could be transcribed and expressed in cells. The cell migration rate and transmembrane number were significantly lower in pEGFP-N1-T-cadherin combined with cisplatin group than those of pEGFP-N1-T-cadherin group (P＜0.05). The cell migration rate and transmembrane number were significantly lower in pEGFP-N1-T-cadherin group than those of control group, pEGFP-N1 group, cisplatin group and pEGFP-N1 combined with cisplatin group (P＜0.05). There were interaction between T-cadherin and cisplatin in inhibiting the migration and invasiveness of CDDP-R B16F10 (P＜0.05). Conclusion T-cadherin gene can restore the inhibitory effect of cisplatin on the migration and invasiveness of cisplatin resistant melanoma cell line. [ABSTRACT FROM AUTHOR]

BACKGROUND: Studies have confirmed that monosialotetrahexosyl anglioside can induce human umbilical cord mesenchymal stem cells to differentiate into neuron-like cells, but little is reported on its optimal concentration. OBJECTIVE: To explore the optimal concentration of monosialotetrahexosyl ganglioside that induces human umbilical cord mesenchymal stem cells to differentiate into neuron-like cells in vitro. METHODS: Human umbilical cord mesenchymal stem cells were isolated by using collagenase digestion method, and after expansion, passage 3 cells were randomly allocated into five groups. When 70%-80% of cells were confluent, 50, 100, 150 and 200 mg/L monosialotetrahexosyl ganglioside induction solutions were added in corresponding experimental groups, while cells in the blank control group were cultured in the same volume of L-DMEM medium. Cell morphology was observed under inverted phase contrast microscope. Expression levels of microtubule-associated protein 2, neurofilament protein and glial fibrillary acidic protein were measured by using immunohistochemistry at 6 hours after induction. RESULTS AND CONCLUSION: Human umbilical cord mesenchymal stem cells were isolated successfully and sub-cultured stably. These cells could express surface markers of mesenchymal stem cells. Monosialotetrahexosyl ganglioside at the optimal concentration of 150 mg/L was confirmed to induce the neuron-like differentiation of human umbilical cord mesenchymal stem cells, and differentiated cells could express microtubule-associated protein 2 and neurofilament protein as neuron-specific markers. [ABSTRACT FROM AUTHOR]

BACKGROUND: Vitamin D is a key substance that promotes calcium absorption and maintains the health of skeletal system, but its relationship with bone mineral density remains controversial. OBJECTIVE: To determine the relationship between serum level of vitamin D and bone mineral density in the middle-aged and elderly patients in southern China. METHODS: The patients aged over 50 years old admitted in the clinic and in-patient department of a large-scale 3A hospital from July 2014 to December 2016 were enrolled and the clinical data were collected. Then, the association between the serum level of 25-hydroxy vitamin D (25(OH)D) and bone mineral density (at the lumbar spine, femoral neck and total hip) was analyzed. RESULTS AND CONCLUSION: A total of 1 154 patients (77.2% female) were enrolled with a mean age of (64.62±10.48) years, and the mean serum level of 25(OH)D was (62.07±37.40) nmol/L. The serum level of 25(OH)D decreased with age increasing, and the proportion of vitamin D insufficiency/deficiency in patients aged 50-59, 60-69 and ≥ 70 years old was 71.3%, 77.0% and 80.1%, respectively. Correlation analysis showed that the serum level of 25(OH)D was not associated with bone mineral density at the lumbar spine, but had a weakly positive correlation with bone mineral density at the femoral neck and total hip (r=0.09 and 0.08, P < 0.01). For patients with vitamin deficiency (25(OH)D ≤ 50 nmol/L), there was a significantly positive correlation between serum level of vitamin D and bone minerat density at the lumbar spine, femoral neck and total hip (r =0.117, 0.120 and 0.146, P < 0.01). After subgroup analysis, vitamin D level was not associated with bone mineral density in male, but was significantly associated with that in female (r=0.105, 0.135 and 0.171 for the lumbar spine, femoral neck and total hip, respectively, P < 0.01). Therefore, the prevalence of vitamin D deficiency is common in middle-aged and elderly patients in southern China. The serum level of vitamin D is not related to bone mineral density in male, but was positively correlated with that in female. [ABSTRACT FROM AUTHOR]

Up to date, simulation of subsonic flows over the high lift device is still a challenge, especially for the cases with higher angles of attack. In order to evaluate the ability of HyperFLOW, an in-house CFD software for subsonic turbulence flow over complex geometries based on structured and unstructured grids, the NASA high lift trap wing model is selected and investigated. Two cases discussed in the NASA high-lift workshop are considered on three types of grids, i.e. two unstructured or mixed grids, and one multi-block structured grid. The simulations on coarse, medium and fine grids for each type are carried out to access the performance of grid convergence. The numerical results are analyzed using Richardson extrapolation method, and compared with the experimental data and the results by other famous CFD codes in the world. The comparison demonstrates that HyperFLOW has a good property of grid convergence on different grids. As the statistical conclusions of the NASA high-lift workshop, the numerical results are acceptable within the linear part of the aerodynamic forces in the regime of lower and medium angles of attack. However, it is still a challenge for the prediction of the cases in higher angles of attack, especially for the cases near stall. [ABSTRACT FROM AUTHOR]