T-钙黏蛋白联合顺铂对黑色素瘤顺铂 耐药株的作用研究.

Objective To investigate the effect of T-cadherin combined with cisplatin on cisplatin resistant malignant melanoma cell line. Methods CDDP resistance B16F10 (CDDP-R B16F10) was induced by using high and gradually increased dose of CDDP．MTT assay was used to test the proliferation of CDDP-R B16F10. The T-cadherin was transfected into CDDP-R B16F10 cells. The expressions of T-cadherin mRNA and protein were measured by reverse transcription polymerase chain reaction (RT-PCR) and SP immunohistochemistry method. There were six groups in this study including control group, pEGFP-N1 group, pEGFP-N1-T-cadherin group, cisplatin group, pEGFP-N1 combined with cisplatin group and pEGFP-N1-T-cadherin combined with cisplatin group. The effects of T-cadherin combined with cisplatin on migration and invasion of CDDP-R B16F10 were determined by Wound-healing assay and Transwell invasion assay. Factor analysis method was used to evaluate the interactions of T-cadherin and CDDP on migration and invasion of CDDP-R B16F10. Results The CDDP-R B16F10 cell line was successfully established. There was no statistical difference in proliferation between CDDP-R B16F10 cells and B16F10 cells (P＞0.05). RT-PCR and SP immunohistochemistry assay showed that Tcadherin could be transcribed and expressed in cells. The cell migration rate and transmembrane number were significantly lower in pEGFP-N1-T-cadherin combined with cisplatin group than those of pEGFP-N1-T-cadherin group (P＜0.05). The cell migration rate and transmembrane number were significantly lower in pEGFP-N1-T-cadherin group than those of control group, pEGFP-N1 group, cisplatin group and pEGFP-N1 combined with cisplatin group (P＜0.05). There were interaction between T-cadherin and cisplatin in inhibiting the migration and invasiveness of CDDP-R B16F10 (P＜0.05). Conclusion T-cadherin gene can restore the inhibitory effect of cisplatin on the migration and invasiveness of cisplatin resistant melanoma cell line. [ABSTRACT FROM AUTHOR]