Search

An analytical method for simultaneous determination of chlorothalonil and its metabolite chlorothalonil-4-hydroxy in fruits and vegetables was developed using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The samples were extracted by acidified acetonitrile, and the extraction solution was analyzed by HPLC-MS/MS equipped with APCI ion source. The results showed that good linearity was obtained in the range of 0.005-0.2 mg/L with correlation coefficients (R² ) greater than 0.99. The limits of quantification were 0.01 mg/kg. At the spiked levels of 0.01, 0.05, and 0.2 mg/kg, the average recoveries in four sample matrices (cabbage, cucumber, grape, and apple) were 83%-104% with the relative standard deviations (RSDs) varying from 0.73% to 7.7% (n=6). The proposed method is simple, rapid, accurate and reliable, and it is suitable for the quantitative determination of chlorothalonil and its metabolite in fruits and vegetables. [ABSTRACT FROM AUTHOR]

Objective　To investigate the prevalence and characteristics of nephropathy in HIV infected patients before antiviral treatment，and to analyze the related factors of nephropathy before antiviral treatment. Methods　Patients with HIV infection who visited the Third People’ s Hospital of Kunming from June 2019 to March 2021 were recruited and screened by urine analysis，serum creatinine，urine microalbumin-to-creatinine ratios，urinary α1 microglobulin，and renal ultrasound. And all patients were screened by triglyceride，total serum cholesterol，fasting plasma glucose test . Body mass index was measured.Demographic data ，medical history   of hypertension and diabetes were collected. SPSS17.0 was used for univariate analysis and logistic regression analysis. Results　The mean age of 468 subjects was 43.42±14.66 years. The male to female ratio was 1.96. The mean body mass index was 21.73±3.76. Body mass index ≥25 accounted for 15% of all the subjects . Some patients smoked daily which accounted for 33.3% of all the subjects. Patients with hypertriglyceridemia，hypertension and diabetes accounted for 24.7%，10.7%，7.7% respectively. And 21.8% of patients were taking drugs which were not antiretroviral therapy. The detection rate of kidney disease in total of 468 cases was 20.7%. the CKD-EPI formula was used to calculate and estimate glomerular filtration rate （eGFR）. Patients with eGFR < 60 mL/min/1.73 m²， 60～90 mL/min/1.73 m²，≥ 90 mL/min/1.73 m2 accounted for 1.1%，9.6%，89.3% respectively. The positivity rate of urinary protein was 3.1%，which measured just only by urine analysis. The urinary microalbumin/creatinine ratio （ACR） ≥ 30 mg/gCr were occurred in 24/111 cases （21.6%）. The Urinary α1 microglobulin ≥ 12.5 mg/L were occurred in 71/111 cases （63.9%）. Multivariate analysis indicated the independent effects of the age ≥ 50 years （OR = 2.285， P = 0.002），body mass index ≥25 （OR = 2.029， P = 0.025），hypertension （OR = 2.334， P = 0.017），diabetes （OR = 2.634， P = 0.014），HIV viral load ≥1×105 copies /mL （OR = 2.296， P = 0.002）， which significantly associated with kidney disease. Female is a protective factor for kidney disease in HIV infected persons before antiviral therapy. Conclusions　The detection rate of kidney disease is high and mainly abnormal urinary protein in Kunming area.Gender，age，hypertension，diabetes，body mass index and HIV viral load are significantly associated with the kidney disease in HIV patients before antiviral treatment. When screening for kidney disease，it is recommended to fully evaluate the results with urine analysis，serum creatinine，renal ultrasound， urinary microalbumin/creatinine ratio，and urinary α1 microglobulin. [ABSTRACT FROM AUTHOR]

In recent years, the development of sound-absorbing materials has advanced rapidly, and been widely used in traffic buildings and other areas. Tn this paper, the characteristics and research progress of sound absorption materials are reviewed, and the basic principle of sound absorption is discussed. Tn addition, starting from the preparation of sound absorbing materials, this paper mainly introduces the research progress of the sound absorbing characteristics and material structure of porous, resonant and composite sound absorbing materials. Tt is a key research direction that how to use the improvement of the internal structure of the material to achieve efficient noise reduction in the field of sound absorbing materials. Finally, the problem about how to correlate the damping loss factor of acoustic materials with the acoustic coefficient is put forward. And the sound absorption effect is studied based on the correlation. So the acoustic performance can be controlled by the design of material damping factor. [ABSTRACT FROM AUTHOR]

目的 分析并总结分化型甲状腺癌 (DTC) 患者术后 131I 全身扫描中非转移性疾病所致假阳性 的分布及原因,为临床医生提供一定的参考意义。方法 回顾性分析西南医科大学附属医院核医学科和内江 市第一人民医院通过相关检查、病理及随访证实的 5 个特殊假阳性病例,所有患者行 131I 全身扫描前均已通过 手术切除甲状腺并通过病理证实为 DTC。结果 2例患者为创伤摄取131I所致假阳性, 2 例为囊肿摄取 131I 所 致假阳性,1例为畸胎瘤摄取 131I 所致假阳性。结论 131I 全身显像时假阳性的情况多种多样,常给诊断带来困 难,所以正确认识假阳性的情况可以为临床提供准确的信息。 [ABSTRACT FROM AUTHOR]

Objective To investigate the predictive and diagnostic value of absolute value and function of different lymphocyte subsets in evaluating the risk of early viral infection after kidney transplantation. Methods Ninety-five kidney transplant recipients were enrolled in this prospective observational cohort study, and divided into the stable group (n=77) and infection group (n=18) according to postoperative immune status. Peripheral blood samples were collected for flow cytometry before operation, and 2 weeks, 1 month, 2 months and 6 months after operation. The dynamic changes of the absolute values of CD4+ T cells, CD8+ T cells and natural killer (NK) cells were compared between two groups. The function of lymphocyte subsets in two groups was evaluated by detecting the proportion of interferon (IFN)-γ+ CD4+ T cells, IFN-γ+ CD8+ T cells and IFN-γ+ NK cells. The value of the absolute values and function of lymphocyte subsets in predicting and diagnosing viral infection in the early stage after kidney transplantation was evaluated. Results During viral infection, the absolute values of CD4+ T cells, CD8+ T cells and NK cells in the infection group were at a relatively low level. At 2 months after operation, the absolute values of CD4+ T cells and NK cells in the infection group were lower than those in the stable group. At 6 months after operation, the absolute values of CD4+ T cells and CD8+ T cells in the infection group were significantly lower compared with those in the stable group (all P<0.05). During viral infection, the proportion of IFN-γ+ CD4+ T cells, IFN-γ+ CD8+ T cells and IFN-γ+ NK cells in the infection group were all at a relatively low level, especially that of IFN-γ+ CD8+ T cells decreased most significantly. At postoperative 2 months, the proportion of IFN-γ+ CD8+ T cells and IFN-γ+ NK cells in the infection group was significantly higher than those in the stable group. At 6 months after operation, the proportion of IFN-γ+ CD4+ T cells and IFN-γ+ CD8+ T cells in the infection group was significantly higher than those in the stable group (all P<0.05). Logistic regression analysis showed that the increasing proportion of IFN-γ+ CD8+ T cells and IFN-γ+ NK cells was correlated with the increasing risk of viral infection at 2 months after operation (both P<0.05). The receiver operating characteristic (ROC) curve demonstrated that the diagnostic value of absolute values of lymphocyte subsets combined with IFN-γ secretion function for viral infection in the immunocompromised recipients was significantly higher than that of absolute values of lymphocyte subsets alone (P<0.05). Conclusions Dynamic monitoring of the changes of absolute values and function of lymphocyte subsets provides critical reference value for the prediction, diagnosis and medication guidance of viral infection. [ABSTRACT FROM AUTHOR]

Objective To explore the effect of the different ways of internal fixation in treatment of elderly patients with femur intertrochanteric fractures. Methods 179 elderly femur intertrochanteric fractures patients were retrospectively analyzed from January 2005 to December 2012. The patients were divided into four groups and given four different operations of internal fixation by using dynamic hip screw Gamma screw proximal femoral nail and proximal femoral nail respectively. The operative time blood loss fracture healing time postoperative joint function recovery postoperative complications were compared among the four groups. Results 179 patients were followed up for 21 days to 6 years with an average of 34 months. The healing time of the group of PFNA is shorter than the other groups but there was no statistically significant difference. The postoperative complications of Coxa vara were found in two cases and one case for the group of DHS and the group of Gamma respectively. And there all had one case of brad cutting for the group of DHS the group of Gamma and the group of PFN. However there was no complication in the group of PFNA the difference was statistically significant （ ＜0.05） ．Conclusions Four internal fixation operations have advantages and disadvantages and should be reasonably choosed based on the patient's condition before surgery underlying diseases type of fracture and its associated injury. However the fracture healing time of the group of PFNA is shorter than the other groups and has better effect so PFNA should be used in treatment of elderly femur intertrochanteric fractures as well as possible. [ABSTRACT FROM AUTHOR]

A single-color steel open shed has been widely used for the main structure of the building in the production of fattening lambs. However, the roof cannot resist the external solar radiation in summer, leading to the increase of temperature in the shed and the occurrence of heat stress of lambs. The objective of this study was to investigate the heat insulation performance of a single-color steel roof that was covered with the reflective film, with emphasis on the physiological and growth performance of fattening lambs. Two sheds with similar building structures were selected, where one was reformed by pasting a reflective film on a single-color steel roof (film shed), and another without reform as a control (control shed). An infrared thermal imager was used to investigate the inner surface temperature of the roof, where the radiant heat of inner surface of the roof was calculated in each stage. Temperature and humidity in both sheds were continuously and dynamically measured, together with the growth performance and physiological indexes of fattening lambs. The trial lasted 67 days from June to August, including the three experimental stages (days 1-7 in the early-stage, days 8-37 in the mid-stage, and days 38-67 in the late-stage). The results showed that: 1) The inner surface temperature of the control shed reached the highest value at 11:30, while the film covered shed reached the peak value at 14:00, indicating a delay by 2.5 hours. The radiant heat of inner surface of the roof also showed the same pattern during the whole period. The inner surface temperature of the film shed was 1.87-14.20 ℃ lower than that of the control (average 7.31 ℃), indicating the most significant decrease (P<0.05) from 9:00-16:30 every day. In addition, the radiant heat of inner surface of the roof in the film shed was 8.85% lower than that of the control, indicating the largest 18.65% decrease in the late-stage at 11:30. 2) In three stages, the ambient temperature in the film shed decreased by 0.55 ℃ on average, compared with the control, especially in the early stage from 9:00 to 13:00, reaching 1.55-1.95 ℃ (average 1.78 ℃) (P<0.05). There was no significant temperature difference between the two sheds in the rest stages (P>0.05). Also, there was a significant linear positive correlation between the ambient temperature and the inner surface temperature of the roof, as well as the radiant heat of inner surface of the roof (P < 0.01). 3) The average daily gain of lambs in the film shed increased by 10.97 % (P < 0.05). There was a significant difference in the amount of water consumption between the fattening lambs in midand late-stage of the test (P < 0.05), which was 15.58 % and 12.66 % higher than that in the control. The respiratory rate of sheep in the film shed decreased by 5.31%, 7.85 times/min lower than the control (P < 0.05) in the middle stage. In summary, the single-color steel roof pasted by the reflective film can be expected to effectively improve the thermal insulation performance of the roof, and then to improve physiological and growth performance in the fattening lambs to relieve heat stress. [ABSTRACT FROM AUTHOR]

In this experiment, summer squash variety' Nongyuan No.1' was used as experimental material, and seven treatments were set up: suitable temperature treatment (day/night 25℃/16℃, CK), watering 1/3 times Yamazaki cucumber formula; Suboptimal temperature treatment (day/night 16℃/8℃, TCK), watering 1/3 times Yamazaki cucumber formula. Under sub-optimal temperature conditions, 1/3 times Yamazaki cucumber formula was used to prepare nutrient solution with HIDS concentration of 100, 400, 700, 1000, 1300mg·L-1(T1, T2, T3, T4 and T5 treatments, respectively). After 18 days of indoor culture in artificial climate, the effects of HIDS on the growth index, osmotic adjustment substances in leaves and MDA of summer squash seedlings were analyzed. The results showed that the growth status, antioxidant enzyme activity and photosynthetic capacity of summer squash treated with TCK were worse than those treated with CK. Under suboptimal temperature, HIDS treatment with different concentrations can alleviate the inhibition of suboptimal temperature on the growth of summer squash seedlings, and the optimum concentration is 700mg·L-1 (T3). Compared with TCK treatment, the leaf area per plant, fresh and dry weight of the whole plant, soluble protein, proline content, POD, SOD and CAT activities are significantly increased, and MDA content is significantly decreased. The total amount of chlorophyll, photosynthetic rate, transpiration rate, stomatal conductance increased significantly; The Fv/Fm, ΦPSⅡ, Fv'/Fm', ETR, qP and NPQ decreased, alleviate the damage caused by suboptimal temperature to photosynthetic organs of summer squash. Therefore, adding suitable concentration of HIDS can improve the adaptability of summer squash seedlings to suboptimal temperature by promoting the accumulation of biomass and osmotic adjustment substances in leaves, reducing the degree of cell membrane lipid peroxidation, and improving the activity of antioxidant enzymes and photosynthetic capacity, and when the concentration of HIDS is 700mg·L-1, each index is closest to CK treatment. [ABSTRACT FROM AUTHOR]

An anti-drift sprayer with a conical wind field has emerged as an innovative structural device for the auxiliary airflow in plant protection operations during crop production. There is also a significant reduction of droplet loss for the effective deposition of fine particles in the target areas. However, an airflow obstruction can be found in the flow channel of the current sprayers, leading to the lower overall performance of the device. In this study, a systematic optimization was made on the inner flow channel in an anti-drift spray device under a conical wind field, thereby clarifying the anti-drift mechanism for better performance of the device. A force model of a single droplet was also constructed for the moving air medium, according to proton dynamics. After that, the CFD numerical simulation and wind field test were utilized to optimize the airflow loss in the inner runner for the better design of the device. The simulation result showed that the disturbance of eddy current was improved without the abnormality of local speed after optimization. Specifically, the conical wind speed at the outlet of the device still reached 17.00 m/s, increasing by 23.5%, compared with that before the optimization. The wind speed test showed that the effective utilization rate of the auxiliary airflow at the outlet of the device was 21.2% higher than that before the optimization, when the radius of curvature of the inner flow channel elbow was designed to be 4 cm, indicating that the optimization plan was feasible. Furthermore, there were significant correlations between the cross wind speed, nozzle height, conical wind speed, and the proportion of total droplet drift under wind tunnel conditions. By contrast, there was a negative correlation between the conical wind speed and the proportion of total droplet drift. More importantly, the proportion of the total droplet drift presented a downward trend, whereas, the adverse effect of the crosswind on the droplet deposition gradually decreased, with the increase of conical wind speed. Additionally, a multi-factor orthogonal experiment was carried out to establish the mathematical model of the total droplet drift ratio in the vertical/horizontal direction. It was found that the cone-shaped wind field significantly reduced the droplet loss in the space with the wind. There was also a higher significance of the vertical/horizontal mathematical model (P<0.05, R2=0.934/0.945). Consequently, the conical wind field can be widely expected to effectively resist the generation of vortexes, thereby reducing the droplet loss with the wind in the vertical height. This finding can also provide a sound reference for the comprehensive analysis of droplet migration and deposition in protected agriculture. [ABSTRACT FROM AUTHOR]

BACKGROUND: In recent years, there were lots of researches on the treatment of diseases by gene modified mesenchymal stem cells, including the treatment of autoimmune diseases. Various miRNAs have been found to be abnormally expressed in autoimmune diseases, among which miR-1 is low-expressed in polymyositis, and miR-1-3p is one of the miRNAs related to systemic lupus eythematosus susceptibility genes. OBJECTIVE: To investigate the immunoregulatory effect of miR-1-5p modified umbilical cord-mesenchymal stem cells on T lymphocyte subsets in systemic lupus erythematosus. METHODS: The expression level of miR-1-5p in the two groups was detected by real-time fluorescence quantitative PCR in eight patients aged 30-60 years with active systemic lupus erythematosus and eight healthy subjects. Umbilical cord-mesenchymal stem cells were isolated and cultured from umbilical cord of newborns delivered at term or cesarean section under aseptic conditions. Using the liposome method, the umbilical cord-mesenchymal stem cells were transfected with Red Fluorescent Control with a concentration gradient of 5, 7, 10, and 15 nmol/L for 24, 48, and 72 hours. Transfection efficiency of miR-1-5p was determined by flow cytometry at different concentration gradients and time gradients. Peripheral blood mononuclear cells in systemic lupus erythematosus patients were isolated by density gradient centrifugation and co-cultured with umbilical cord-mesenchymal stem cells which were transfected with miR-1- 5p, and divided into four groups: umbilical cord-mesenchymal stem cells transfected with miR-1 and co-cultured with peripheral blood mononuclear cells of systemic lupus erythematosus patients, umbilical cord-mesenchymal stem cells transfected with miRNA negative control and co-cultured with peripheral blood mononuclear cells of systemic lupus erythematosus patients, umbilical cord-mesenchymal stem cells co-cultured with peripheral blood mononuclear cells of systemic lupus erythematosus patients, and peripheral blood mononuclear cells of systemic lupus erythematosus patients without treatment group. The coculture was conducted for 48 hours. The relative expression levels of IL-17A, Foxp3, IFN-γ and IL-4 genes in each group were detected by real-time fluorescence quantitative PCR. Flow cytometry was used to detect the differentiation of Th17, Treg, Th1 and Th2 cells in peripheral blood mononuclear cells of each group. RESULTS AND CONCLUSION: (1) miR-1-5p was significantly down-regulated in peripheral blood mononuclear cells of systemic lupus erythematosus patients compared with healthy controls (P < 0.05). (2) The transfection efficiency was highest when the concentration of miR-1-5p was 10 nmol/L and the transfection time was 48 hours. (3) Compared with the peripheral blood mononuclear cells of systemic lupus erythematosus patients without treatment group, the expression of Foxp3 in umbilical cord-mesenchymal stem cells transfected with miR-1-5p and co-cultured with peripheral blood mononuclear cells of systemic lupus erythematosus patients group was increased; the proportion of Treg cells was significantly increased; and the differences were statistically significant (P < 0.05). Compared with the peripheral blood mononuclear cells of systemic lupus erythematosus patients without treatment group, Th17/Treg ratio decreased in the other three co-culture groups, and the difference was statistically significant (P < 0.01). Th17/Treg cell ratio detected by flow cytometry in each group was not significantly different (P > 0.05). Compared with the peripheral blood mononuclear cells of systemic lupus erythematosus patients without treatment group, the Th1/Th2 ratio in the umbilical cord-mesenchymal stem cells transfected with miR-1-5p and co-cultured with PBMC of systemic lupus erythematosus patients group decreased, and the difference was statistically significant (P < 0.05). However, the Th1/Th2 cell ratio of each group detected by flow cytometry was not significantly different (P > 0.05). (4) The results showed that the umbilical cord-mesenchymal stem cells modified by miR-1-5p had an immunoregulatory effect on Treg cells of systemic lupus erythematosus, and overexpression of miR-1-5p could promote the differentiation and proliferation of Treg cells of systemic lupus erythematosus patients. miR-1-5p regulates the immune imbalance of Th17/Treg and Th1/Th2 by up-regulating the expression of Treg cells. [ABSTRACT FROM AUTHOR]

To determinate potential strategy of nitrogen-fixing plants adapting to soil nitrogen change in karst region, two nitrogen-fixing plants (Albizia odoratissima and Cajanus cajan) were selected to explore this issue in experiment. The results showed that TP, AP, NH+4 -N contents in rhizosphere soil of two nitrogen-fixing plants in nitrogen application soils were significantly higher than those in blank group soils, except NO-3 -N contents. The total biomass of karst nitrogen-fixing plants increased, but the root-shoot ratios and nitrogen fixation rates decreased under the nitrogen application soils. The two nitrogen-fixing plants had the facultative nitrogen fixation strategy in karst region. In blank group soils, the root-shoot ratio, N and P contents of roots, N contents of stems and leaves, nitrogen fixation rate, arbuscular mycorrhizal fungi (AMF) colonization, and root phosphatase activity of A. odoratissima were higher than those parameters of C. cajan. In nitrogen application soils, the AMF colonization and root phosphatase activity of C. cajan were higher than those parameters of A. odoratissima. The above results suggested that A. odoratissima might be applicable in adding into the artificial plant community of early vegetational successions when some ecological restoration projects were carried out in this region, while C. cajan might be applicable in adding into the community of later vegetational successions. [ABSTRACT FROM AUTHOR]

Serious vibration in most mechanical equipment has posed a great impact on the production efficiency of agriculture goods and the service life of key parts. Much efforts have been made on the influence of external excitation on the vibration generation, transmission, and even reduction at present. However, there are only a few studies on the influence of internal excitation on the vibration in the ring mold granulator, such as the ring mold and pressing roller. Taking the gear reducer of 420 ring mold granulator as the research object, this study aims to analyze the dynamic characteristics on the complex transmission, large vibration, and short service life of key parts. The time-varying meshing stiffness and meshing error of helical gears were attributed to the internal excitation of transmission system. A cumulative integral potential energy was used to calculate the time-varying meshing stiffness of gear reducer in a ring mold granulator, thereby to determine the variation of meshing stiffness. Then, a six degree of freedom (DOF) dynamic model was established to determine some parameters for the helical gear transmission system in a ring mold granulator. Since the dynamic response clearly reflected the gear vibration, a modal analysis of transmission was made to effectively avoid the nonlinear dynamic resonance. At the same time, a systematic evaluation was carried out to explore the effects of meshing stiffness and comprehensive meshing error on the dynamic response of helical gear transmission. More importantly, the obtained meshing stiffness of helical gear was utilized to optimize the dynamic response of system for a higher installation accuracy in regular maintenance. Furthermore, a finite element (FE) software was used to verify the accuracy of natural frequency in the dynamic model. A spindle torque test of granulator was also carried out to measure the torque time-domain curve. Fourier transform (FT) was then utilized to transformed into the power spectrum curve. A spectrum analysis demonstrated that there was no resonance of main motor with the transmission system, although the complex internal excitation of a granulator from the torque fluctuation. Additionally, an acceleration vibration test was performed on the whole machine and helical gear transmission. The curve of vibration time domain was measured to transform into the vibration frequency domain. The vibration characteristics of helical gear transmission were analyzed to verify the internal excitation in the whole granulator. Finally, the curve of vibration time-domain was integrated to obtain the displacement fluctuation. The test and theoretical calculation of dynamic response indicated that the internal excitation was an important reason for the vibration of whole machine. [ABSTRACT FROM AUTHOR]

The alpine meadow ecosystems of the Zoige Plateau play important roles in the energy and water cycle of the Qinghai-Tibet Plateau, but observation data of this region regarding to surface energy and water fluxes are very scarce. In this study, annual flux measurements were conducted based on the eddy covariance technique at a typical alpine meadow on the Zoige Plateau by using a three-dimensional sonic anemometer and an infrared open-path carbon dioxide and water vapor analyzer. The surface energy and evapotranspiration (ET) fluxes were calculated at the basis of half-hour. The purpose of this study is to reveal the characteristics and influencing factors of surface energy exchanges and ET. The results are as follows. All energy flux components showed clear diurnal and seasonal variation patterns. The annual mean net radiation, sensible heat, latent heat, and soil heat fluxes were 94.5, 21.0, 51.8 and 1.2Wm-2, respectively. The energy fluxes showed a "single peak" diurnal variation pattern both in the growing season and the non-growing season, despite different peak times for different energy components. During the non-growing season, the sensible heat fluxes were slight larger than the latent heat fluxes, while the latter absolutely dominated during the growing season. The annual mean Bowen ratio and energy closure rate were 0.70 and 0.77, respectively. Radiation was the most important environmental factor that influenced the sensible heat fluxes, while temperature, radiation and water vapor pressure deficit for the latent heat fluxes. The ET fluxes during the growing season were significantly larger than those during the non-growing season. The daily ET fluxes ranged from 0.12 to 5.09mmd-1, and the annual mean value was 1.82mmd-1. Evapotranspiration during the non-growing season is controlled by the surface conductivity; during the growing season it is dominated by the radiation, rather than the surface conductivity of soil and vegetation. On a seasonal scale, the dynamics of ET depended on the seasonal variation of precipitation. The annual accumulated precipitation and ET were 682.7mm and 673.6mm, respectively, of which the growing season accounted for 84% and 82%. The lack of precipitation from June to July constrained the ET fluxes. During this period, soil water storage became the main source of ET. All precipitation finally returned to the atmosphere through ET. In this study, the seasonal variation patterns of the surface energy fluxes and ET were similar to those reported by other measurement studies on the Qinghai-Tibet Plateau. However, the annual mean Bowen ratio and annual accumulated ET were the largest among these studies. Such results were jointly attributed to the effects of temperature, precipitation, surface vegetation and other factors. Data of this study could be used for the parameterization optimization of the land surface models and for the validation of satellite and remote sensing data in the Zoige region. [ABSTRACT FROM AUTHOR]

Objective To explore the anesthesia management of interventional treatment for congenital heart disease in children. Methods Patent ductus arteriosus （343 cases），atrial septal defect （428 cases），10 years of age undergoing elective septal defect （229 cases） of the children underwent interventional therapy， were randomly divided into ketamine with midazolam group and ketamine with propofol group. Results The differences in the mean arterial pressure during operation， heart rate， respiratory rate， and oxygen saturation were not statistically significant in each group before and after anesthesia （ > 0.05）， and differences in adverse reaction and complications during operation were not statistically significant between the two groups （ > 0.05） . All patients were spontaneous breathing， and interventional treatment was completed successfully. Conclusion Ketamine with midazolam or ketamine with propofol anesthesia methods achieved satisfactory effect in the occlusion of congenital heart disease with fewer complications， safe and effective， faster recovery of anesthesia， and no clinically significant difference. [ABSTRACT FROM AUTHOR]

Anatase TiO2 thin films with different morphology were prepared on FTO substrates by hydrothermal method. With the increasing of hydrochloric acid concentration in the precursor, TiO2 thin films gradually evolved from spherical particle films into TiO2 nanosheet array films with a large percentage of high-energy exposed (001) planes. By analyzing the evolution rule of morphology and X-ray diffraction patterns, a reasonable growth and evolution mechanism of TiO2 thin film with different morphology was proposed, and the role of hydrochloric acid was explained. In order to further improve the performance of TiO2 thin films, CdS quantum dots (QDs) sensitization was performed on different TiO2 thin films by the successive ionic layer adsorption and reaction method. The optical absorption performance and photoelectrochemical (PEC) properties of composite films were studied by ultraviolet visible absorption spectroscopy and three-electrode electrochemistry system. The optical absorption data and PEC performance data show that the properties of CdS / TiO2 composite films are better than those of pure TiO2 thin films, and the properties of nanosheet array films are obviously better than other morphologies, which illustrates the performance superiority of TiO2 nanosheet array films with large area high-energy (001) planes exposed. The excellent PEC properties also suggest that the QDs sensitized TiO2 nanosheet array films with a large percentage of high-energy surfaces have potential applications in PEC solar cells. [ABSTRACT FROM AUTHOR]

The dialogue system has become an important human ‐computer interaction interface in artificial intelligence. Chatbots technology plays a vital role in the development of dialogue system technology and represents the frontier development of dialogue system technology. In this paper, we analyze the emerging of chatbots and recent trends in chatbots. When introducing the research progress of chatbots technology in academia and industry, special emphasis is placed on the three key technologies of chatbots. They include multi ‐ turn response selection model in retrieval ‐based chatbots, response generation model in generation ‐ based chatbots and dialogue model based on deep integration of retrieval and generation. The future develop‐ ment is prospected by analyzing the problems in the chatbots technology. [ABSTRACT FROM AUTHOR]

BACKGROUND: The main reason for the failure after total hip arthroplasty is aseptic loosening of the prosthesis. The traditional acetabular cup fixation method is still controversial in maintaining the initial stability of the acetabular cup. It is a new method that can be considered to further improve the fixation method of the acetabular cup to increase its stability. OBJECTIVE: To analyze the feasibility and superiority of locking screw assisted acetabular cup fixation. METHODS: The digital model of acetabular cup and screws were reconstructed and assembled by software Solidworks. The Von-Mises and shear stress of the acetabular cup and screw by three methods, including acetabular cup, acetabular cup with ordinary cancellous bone screw, and acetabular cup with locked cancellous bone screw, were compared. RESULTS AND CONCLUSION: (1) The finite element model of the established acetabular cup and screw had excellent geometric similarity and could be used for finite element analysis. (2) The acetabular cups with locking screws received less Von-Mises and shear stress than other methods. (3) The locking screws could bear higher stress than ordinary screws and effectively dispersed the shear stress of acetabular cups. (4) It is concluded that the combination of locking screws to fix the acetabular cup can effectively prevent the instability of the acetabular cup and the loosening of the prosthesis; it can better provide stability for the acetabular cup and the mechanical environment that the bone tissue grow in the later stage. The acetabular cups with locking screws have obvious advantages than other ordinary screw, which has the limited bearing capacity and low anti-acetabular cup shear force. [ABSTRACT FROM AUTHOR]

The black silicon material with high-efficiency anti-reflection microstructure has important applicat ion value in the field of optoelectronics. In this paper, two-step metal-assisted chemical etching was used to prepare the "pyramid" silicon and "pyramid-nanowire" micro-nano composite black silicon materials. The field emission scanning electron microscope, near-infrared spectrometer ind Raman spectroscopy were used in turn to charracterize the microscopic morphology, reflectivity and structural properties of the samples. The quantum confine ment model ( QCM) was used to explain the mechanism of the obvious enhancement of the Raman spectrum scattered light intensity of black silicon, and the light trapping model and multilayer film interference theory were used to ;malyze the anti-reflection mechanism of the black silicon microstructure. The results show that the average reflectivity of the black silicon material, with the micro-nano composite structure, in the band of 900 nm~l 700 nm was 11.94%, which was reduced by 77.:32% and 54.95% compared with the pyramidal silicon and the polished silicon wafer, respectively. The analysis of Raman spectroscopy shows that there was no change in the black silicon material compositions before and ;1fter etching. It was still single crystal silicon wa fer. The nano-swle microstructure on the surface of the black silicon incre;1sed the intensity of the Raman spec trum scattered light. The decrease in reflectivity of black silicon was due to the fact that the incident light was repeatedly reflected inside the micro-nano structure. /\lso, the refractive index gradient layer was established between the micro-nano st ructure to reduce the reflection caused by the sudden change in refractive index. The research conclusion had reference value for improving the laser absorpt ion efficiency of functional nrnteri;ds in the near-infrared band. [ABSTRACT FROM AUTHOR]

BACKGROUND: Percutaneous kyphoplasty for multi-segmental spinal metastases has some problems, such as long operation time, high fluoroscopic dose, and easy leakage of bone cement. Robot-guided percutaneous kyphoplasty can optimize surgery and reduce the incidence of complications. OBJECTIVE: To evaluate the safety and advantage of robot-guided percutaneous kyphoplasty in treatment of multi-segmental spinal metastases. METHODS: The clinical data of 43 cases with multi-segmental spinal metastases with no signs of nerve injury from January 2018 to April 2019 were analyzed retrospectively. According to the different operation methods, the patients were divided into robot-guided group (22 cases) and traditional fluoroscopy group (21 cases). There was no significant difference in gender, age, number of diseased vertebrae, source of primary tumor and preoperative visual analogue scale score between the two groups (P > 0.05), with comparability. The data of operation time, fluoroscopy frequency, fluoroscopy dose, the incidence rate of pedicle wall breaking, angle of penetration, and leakage of bone cement were collected in both groups. Visual analogue scale score, the midline vertebral height and Cobb angle were compared preoperatively, 2 days postoperatively, and during the final follow-up between the two groups. RESULTS AND CONCLUSION: (1) The number of diseased vertebrae in the robot-guided group and traditional fluoroscopy group was 79 and 70, respectively. (2) The average operation time, fluoroscopy frequency and fluoroscopy dose in the robot-guided group were significantly lower than those in the traditional fluoroscopy group, and the difference was statistically significant (P < 0.001). (3) The incidence rate of pedicle wall breaking was 6% in the robot-guided group and 21% in the traditional fluoroscopy group, and the difference was statistically significant (χ2 =6.040, P=0.014). (4) The angle of penetration in the robot-guided group was larger than those in the traditional fluoroscopy group (P < 0.001). (5) The leakage rate of bone cement in the robot-guided group was lower than in the traditional fluoroscopy group (8%, 23%,χ2 =6.869, P=0.009). (6) There was no significant difference in visual analogue scale score, the midline vertebral heights and Cobb angle between the two groups before and 2 days after operation and during the final follow-up (P > 0.05). (7) It is indicated that robot-guided percutaneous kyphoplasty in treatment of multi-segmental spinal metastases can reduce operation time, reduce fluoroscopy frequency, reduce fluoroscopy dose, have good accuracy of penetration and low leakage rate of bone cement. [ABSTRACT FROM AUTHOR]

Two domestic stock market disasters in 2008 and 2015 were selected as the background to construct China’s stock market network community structures before， during and after the disasters. The node systemic importance index and the systemic risk index of stock market were constructed to analyze the core stocks， industries， stock portfolios and their changes within the network communities in each period， and to explore the correlation between systemic risks and network topological indicators and macroeconomic indicators. The results showed that the industrial sector suffer severe setbacks， and the raw materials， financial real estates， medical and health sectors play a role in protecting the market and repairing stock indexes during the stock market disasters. Three kinds of special community structures are found and some of them have a tendency to merge with each other. During the periods of extreme stock index fluctuations， the systemic risk of China’s stock market is significantly correlated with some network topological indicators and macroeconomic indicators. [ABSTRACT FROM AUTHOR]

Objective To investigate the clinical efficacy of interventional therapy of residual shunts after cardiac surgery of congenital heart disease（CHD）．Methods From January 2004 to April 2011，10 patients （6 males and 4 females） with residual shunt after cardiac surgery underwent transcatheter intervention therapy， including 2 patients with residual shunt after surgery of patent ductus arteriosus（PDA）， 7 patients with residual shunt after surgery of ventricular septal defect（VSD），and 1 patient with residual shunt after surgery of atrial septal defect （ASD）．Their mean age was（27.6±10.8） years，and mean weight was（59.2±7.6） kg. Then，their transthoracie echocardiography （TTE） and electrocardiogram （ECG） were undertaken at postoperative 2 days， l month， 3 months，6 months and 12 months five time points．ResuIts All 10 patients were treated successfully. No patient encountered complications during follow-up．Conclusion Interventional therapy of residual shunts after surgery of CHD was safe，effective and feasible. [ABSTRACT FROM AUTHOR]

Objective:To explore the entry point of pharmaceutical care by clinical pharmacists for the heart failure patients with preserved ejection fraction, and the methods of participating in the formulation of drug treatment regimen and pharmaceuti - cal care. Methods: Clinical pharmacists were involved in the treatment of 3 heart failure patients with preserved ejection fraction. During the course of pharmaceutical care, clinical pharmacists helped physicians to improve treatment regimens, put forward suggestions on medication and provide pharmaceutical care according to patients conditions. Results: The symptoms of the patients were significantly improved, heart failure was co nt rolled, and the patients were duly discharged after their conditions were stable. Conclusion:Clinical pharmacists combine their professional knowledge of pharmacy with the first--line clinical practice, which plays a positive and important role in drug treatment efficacy, and is conducive to the effective and safe medication of patients. [ABSTRACT FROM AUTHOR]

We used Xinong 9940 and Qianku 3 as materials to set three nitrogen fertilizer treatments levels(N1)90,(N2)180,( N3)270 kg·hm-2, to discuss the effects of different nitrogen application rates on the growth, dry matter accumulation and transport of vegetative organs and nitrogen application rate on grain filling characteristics and yield of two Tartary buckwheat. The results were as follows.(1)Nitrogen fertilizer significantly promoted the growth and development of Tartary buckwheat. With the increase of nitrogen application, the plant height, SPAD value and dry matter accumulation of Tartary buckwheat showed a gradual increase trend, reached the maximum value under N3 treatment, significantly higher than N1 and N2 treatment. Under the same nitrogen application conditions, Qianku 3 was better than Xinong 9940. As far as the translocation rate is concerned, the two varieties of Tartary buckwheat perform inconsistently. Nitrogen application significantly increased the stem and leaf dry matter transport rate of Xinong 9940, while Qianku 3 did the opposite.(2)With the increase of nitrogen application, the day of filling duration of Tartary buckwheat increased, the maximum grain filling date was prolonged, the mean grain flling rate decreased, and the 100-grain weight showed a downward trend. Under the same nitrogen applicaton conditions, compared with Qianku 3, Xinong 9940 had a fasted flling rate and greater grain weight.(3)With the increase of nitrogen application, yield and its constituent factors increased first and then decreased, but the varieties were different. The yield of Xinong 9940 reached the highest in N2 treatment, which was 1 650.0 kg·hm-2,which was 45.6% and 28.2% higher than that of N1 and N3 treatments. The yield of Qianku 3 reached the highest in N1 treatment, which was 616.7 kg·kg-2, which increased yield by 12.8% and 51.6% compared with N2 and N3. It was suggested that in the dry farming area of the Loess Plateau, buckwheat breeding and planting depend on the variety of buckwheat, and the optimum amount of nitrogen application should be selected, and the best nitrogen rate of Xinong 9940 was 180～270 kg·hm-2, and the optimum nitrogen rate of Qianku 3 was 90～180 kg·hm-2. [ABSTRACT FROM AUTHOR]

Objective To explore the predictive value of the index of cardio-electrophysiological balance(iCEB) in patients with an implatable cardioverter-defibrillator(ICD) for primary prevention of sudden cardiac death (SCD).Methods The pacemaker data of patients with ICD implantation for primary prevention of SCD were collected by pacemaker program controller.These cases were divided into two groups according to whether the cardiovascular events (ventricular tachycardia or ventricular fibrillation) happened or accepted appropriate ICD treatment. The iCEB (iCEB≈QT/QRS) was calculated by routine 12-lead ECG parameters QRS and QT, and the difference between the two groups was analyzed.ResultsThe area under the ROC curve of QT/QRS was 0.810（95%CI 0.707-0.913）. And the sensitivity and specificity are 100% and 78% respectively when QT/QRS≥5.0.Conclusion QT/QRS is of clinical value in predicting the effectiveness of ICD implantation for primary prevention of SCD. [ABSTRACT FROM AUTHOR]

In grain harvesting, the combine harvester’s header need to guide along the harvest boundary in time to ensure the harvester is working in full widt, which requires the operator has high operational skills. In addition, with the long-time operation the driver is prone to fatigue, which brings safety hazards to agricultural production. Therefore, it is of great significance to study the automatic navigation technology of combine harvester to reduce the labor intensity of drivers and improve production efficiency. The key of automatic navigation is the extraction of navigation line. Due to richer environmental information, wider detection range and more complete information, visual-based navigation methods have attracted extensive attention. However, the contrast between cut and uncut areas of mature wheat in the image is extremely low under strong illumination, which leads that the harvest boundary of crop is quite blurred. To solve the problem that the cut edge is difficult to extract under low contrast conditions, a fast and accurate navigation line extraction method of combine harvester based on region growing algorithm is presented in this paper. Firstly, the color images of harvesting scene collected by camera was converted into the gray scale image, and the Gaussian filtering was applied to remove the image noise. Then, the region growing algorithm was used to segment the image. Initial seed was selected based on some criteria and then the uncut wheat area was segmented by region growing process. The gray value of each 4-neighboring pixel was compared with the mean gray value of the seed region, if their difference was smaller than the threshold the corresponding pixel was added to the seed region. This procedure was repeated until no pixel could be grouped in the region. To improve the robustness of the region growing algorithm, an adaptive threshold selection method based on gray histogram was proposed. The multi-peak Gaussian fitting of the gray histogram was performed and half of the absolute value of the difference between mean values of the two Gaussian components was taken as the threshold of region growing, then the segmented binary image was processed by the morphological operations to fill the small holes in the segmented region which made the harvest boundary of wheat smoother. Finally, the harvest boundary of crop was detected and the harvester navigation line was acquired by fitting the harvest boundary with the least squares method. The experimental results showed that even though the contrast of cut and uncut wheat areas was extremely low, the proposed method could accurately detect the wheat harvest boundary and extract the harvester navigation line. Under different operating conditions such as different light intensity and different crop growth density, the average angle error between the navigation line extracted by the proposed method and the manually calibrated navigation line was less than 1.21 °, processing a 900×1 200 pixels image took about 0.4 s, which basically meets the real-time requirements of the combine harvester navigation. The results can provide a reference for the automatic navigation of the combine harvester. [ABSTRACT FROM AUTHOR]

The chiral oligomers/polymers are usually used as the scaffolds or templates to induce the helicity/chirality of achiral substances, which has been one of the research hotspots in recent years. Compared to traditional methods, this method can not only avoid the use of expensive chiral reagents and complex synthetic routes, but also enrich the types of chiral substances, which has potential applications in bioscience, especially in the field of exploring the origin of chirality. In this review, we briefly introduce the historical development of chiral assembly of achiral substances induced by natural/synthetic chiral oligomers/ polymers. Additionally, the advances in chiral assembly of achiral polymers and inorganic compounds induced by chiral oligomers/polymers scaffolds (templates) strategies are also discussed. The research focuses and development trends of this method are summarized in the end. With implications for biological helices and functions, further applications of chiralityresponsive polymers based novel chiral materials as enantioselective catalysts and adsorbents will be an interesting and important challenge. In the future, oligomer/polymer scaffolds (templates) strategies will receive much more attention from scientists in the fields of photoelectric materials, chiral resolution, enantiomeric sensors and asymmetric polymerization. [ABSTRACT FROM AUTHOR]

Objective: To investigate the role of autophagy in Hematoporphyrin monomethyl ether (HMME)-mediated Sonodynamic therapy (SDT) in inhibiting the proliferation of C6 glioma cells. Methods: C6 glioma cells grown in log phase were randomly divided into four groups: control group (not treated), ultrasound group (individual ultrasound irradiation), HMME group (HMME alone), SDT group (ultrasound irradiation + HMME). The number of autophagic vacuoles in SDT treated C6 glioma cells was observed by trans-mission electron microscopy. The effects of SDT treatment on the expression of LC3, Beclin1, Bcl-2 in C6 glioma cells were analyzed by qRT-PCR and Western blot. MTT was used to detect the viability of C6 glioma cells. Results: Transmission electron microscopy analysis demonstrated that the number of autophagy in SDT group was significantly higher than that in the control group. The mRNA and protein levels of microtubule associated protein 1 light chain 3 (LC3) and Beclin1in C6 glioma cells of SDT group were higher than those of the control group, and the levels of B cell lymphoma-2(Bcl-2) mRNA and protein were lower than those of the control group. Compared with the control group, the viability of C6 glioma cells in SDT group decreased from 0 h to 6 h, and increased from 12 h to 72 h. The viability of C6 glioma cells after 3-methyladenine (3-MA)+SDT and Chloroquine (CQ)+SDT treatment was significantly lower than that of SDT group. Conclusion: SDT may inhibit the proliferation of C6 glioma cells by inducing autophagy. [ABSTRACT FROM AUTHOR]

The balance between bone formation and bone resorption maintains the homeostasis of bone remodeling. Aging, estrogen deficiency and other factors lead to the imbalance between the oxidation system and antioxidant system in the body, Resultsing in increased production of reactive oxygen species and oxidative stress. Recent studies have found that oxidative stress induced by reactive oxygen species plays an important role in bone formation and absorption during bone remodeling. Excessive reactive oxygen species affects the nuclear gene transcriptions through regulation of the cytokine expressions, enzyme activity and related signaling pathways, leading to the proliferative and functional abnormalities of bone marrow mesenchymal stem cells, osteoblasts, osteocytes and osteoclasts. The imbalanced bone remodeling may lead to metabolic bone diseases including osteoporosis. Therefore, this article summarized the sources of oxidative stress and its effects on bone marrow mesenchymal stem cells, osteoblasts, osteocytes and osteoclasts as well as the anti-oxidative treatment of osteoporosis in recent years, which is of great significance to further understand the physiological and pathological mechanism of oxidative stress in bone remodeling. [ABSTRACT FROM AUTHOR]

A two-step method was used to prepare an Al-Cr gradient composite coating on the surface of Q235 steel. First, Cr coating was prepared on the surface of Q235 steel by electroplating, and then Al coating was prepared by hot dip plating. The effects of plating auxiliary, hot dip temperature and time on the coating were studied. The microstructure, thickness, hardness and composition of the coating were analyzed by OM, SEM, EDS, XRD and microhardness test. The corrosion resistance of the coating was evaluated by a full immersion test. The results show that the surface of the coating prepared by 10% NH4Cl+27%ZnCl2 is flat and uniform. The structure is compact and no defects such as spalling and leakage plating appear. The microstructure and thickness of the coating change under different hot dip process. A three-layer structure（Al layer, Al-Cr alloy layer, Al-Cr-Fe alloy layer） of the coating is converted into a two-layer structure（Al layer, Al-Cr-Fe alloy layer） by. The thickness of the Al layer in the coating decreases with the increase of hot dip temperature, while the thickness of the alloy layer shows an increasing trend. As the hot dip time is extended, the thickness of both the Al layer and the alloy layer is increased. The Al-Cr gradient composite coating has better salt water corrosion resistance than the uncoated steel sheet and the hot-dip aluminum-plated steel sheet, and the corrosion rate is about 1/5 of that of the uncoated sample. [ABSTRACT FROM AUTHOR]

In order to improve the identification ability of fish meal quality detection device based on bionic olfaction, in this paper, we used the developed fish meal quality detection device based on bionic olfaction to extract response characteristic information of fish meal samples, and performed multi-characteristic data fusion optimization on its sensor array. Firstly, according to the response curve of each sensor to the sample, the sensor features (10×6) were extracted to form the original feature matrix, then the normalization method was adopted to normalize the sensor features, and after that, compactness was taken as the standard to evaluate the rationality of the feature selection methods. Three single feature ranking methods (MIC, c2、F-test), three multi-feature ranking methods (RF, LR, SVM) and four recursive feature elimination methods (RFRFE, SVMRFE, DTRFE, LRRFE) were selected to carry out classification accuracy tests on fish meal with different quality. The experimental results showed that in the single feature ranking method, the MIC method had the best classification accuracy of 98.3 %, the number of features was 55, the Chi - square method had the best classification accuracy of 98.9 %, the number of features was 40, the F-test method had the best classification accuracy of 98.3 %, and the number of features was 50, thus the Chi - square feature selection method was more compact than the other two single feature selection methods. In the multi-feature ranking methods, the best classification accuracy rate of RF method was 98.3 %, the number of features was 38, the best classification accuracy rate of LR method was 83.3 %, the number of features was 24, the best classification accuracy rate of SVM method was 92.2%, and the number of features was 33. Therefore, RF feature selection method was more compact than the other two multi-feature selection methods. In recursive feature elimination, the best classification accuracy rate of RFRFE method was 98.3 %, the number of features was 33, the best classification accuracy rate of SVMRFE method was 92.2%, the number of features was 34, the best classification accuracy rate of DTRFE method was 95.6 %, the number of features was 22, the best classification accuracy rate of LRRFE method was 83.9 %, and the number of features was 37. From this, it could be seen that DTRFE and LR feature selection methods had the least number of features, but the classification accuracy rate was low. However, the RFRFE feature selection method was relatively more compact. The random forest-based recursive feature elimination algorithm (RFRFE) was adopted to select the original features, and the best classification accuracy was 98.3%, at this time, the number of features was 33. The idea of this feature selection method was to repeatedly build the model, then select the worst features, put the selected features aside, and then repeat the process on the remaining features until all features had been traversed. The order in which features were eliminated in this process was the order of features. Therefore, this was a greedy algorithm to find the optimal feature subset, while the RFRFE method selected the random forest (RF) as the base model, and obtained the optimal subset by obtaining the best classification accuracy rate. The number of features optimized by RFRFE feature selection method was 33, which reduced the number of features by 45% and greatly reduced irrelevant and redundant information for fish meal quality classification. The characteristics of the optimized sensor array had changed obviously. The sensor array had changed from the original 10 to 8 and sensor 4 (TGS2620) and sensor 6 (TGS2600) had been removed. This showed that these sensors had little contribution to the classification of fish meal quality using RF classifier. Of the six selected features values, only sensor 1 (TGS822), sensor 3 (TGS813) and sensor 5 (MQ136) had selected all the characteristic values, which showed that these sensors played an important role in the classification of fish meal quality by using RF classifier. By using 10 fold cross validation, the RFRFE algorithm was verified to be more compact again. The feature selection method provided a new method and reference for feature optimization of identifying other animal-derived raw material samples by bionic olfaction technology. [ABSTRACT FROM AUTHOR]

In order to improve the identification ability of fish meal quality detection device based on bionic olfaction, in this paper, we used the developed fish meal quality detection device based on bionic olfaction to extract response characteristic information of fish meal samples, and performed multi-characteristic data fusion optimization on its sensor array. Firstly, according to the response curve of each sensor to the sample, the sensor features (10×6) were extracted to form the original feature matrix, then the normalization method was adopted to normalize the sensor features, and after that, compactness was taken as the standard to evaluate the rationality of the feature selection methods. Three single feature ranking methods (MIC, c2, F-test), three multi-feature ranking methods (RF, LR, SVM) and four recursive feature elimination methods (RFRFE, SVMRFE, DTRFE, LRRFE) were selected to carry out classification accuracy tests on fish meal with different quality. The experimental results showed that in the single feature ranking method, the MIC method had the best classification accuracy of 98.3 %, the number of features was 55, the Chi - square method had the best classification accuracy of 98.9 %, the number of features was 40, the F test method had the best classification accuracy of 98.3 %, and the number of features was 50, thus the Chi - square feature selection method was more compact than the other two single feature selection methods. In the multi-feature ranking methods, the best classification accuracy rate of RF method was 98.3 %, the number of features was 38, the best classification accuracy rate of LR method was 83.3 %, the number of features was 24, the best classification accuracy rate of SVM method was 91 %, and the number of features was 55. Therefore, RF feature selection method was more compact than the other two multi-feature selection methods. In recursive feature elimination, the best classification accuracy rate of RFRFE method was 98.3 %, the number of features was 33, the best classification accuracy rate of SVMRFE method was 92.2 %, the number of features was 34, the best classification accuracy rate of DTRFE method was 95.6 %, the number of features was 22, the best classification accuracy rate of LRRFE method was 83.9 %, and the number of features was 37. From this, it could be seen that DTRFE and LR feature selection methods had the least number of features, but the classification accuracy rate was low. However, the RFRFE feature selection method was relatively more compact. The random forest-based recursive feature elimination algorithm (RFRFE) was adopted to select the original features, and the best classification accuracy was 98.3%, at this time, the number of features was 33. The idea of this feature selection method was to repeatedly build the model, then select the worst features, put the selected features aside, and then repeat the process on the remaining features until all features had been traversed. The order in which features were eliminated in this process was the order of features. Therefore, this was a greedy algorithm to find the optimal feature subset, while the RFRFE method selected the random forest (RF) as the base model, and obtained the optimal subset by obtaining the best classification accuracy rate. The number of features optimized by RFRFE feature selection method was 33, which reduced the number of features by 45% and greatly reduced irrelevant and redundant information for fish meal quality classification. The characteristics of the optimized sensor array had changed obviously. The sensor array had changed from the original 10 to 8 and sensor 4 (TGS2620) and sensor 6 (TGS2600) had been removed. This showed that these sensors had little contribution to the classification of fish meal quality using RF classifier. Of the six selected features values, only sensor 1 (TGS822), sensor 3 (TGS813) and sensor 5 (MQ136) had selected all the characteristic values, which showed that these sensors played an important role in the classification of fish meal quality by using RF classifier. By using 10 fold cross validation, the RFRFE algorithm was verified to be more compact again. The feature selection method provided a new method and reference for feature optimization of identifying other animal-derived raw material samples by bionic olfaction technology. [ABSTRACT FROM AUTHOR]

Objective To evaluate the efficacy of aspirin plus clopidogrel in therapy for new-onset migraine attacks following atrial septal defect (ASD) closure, to analyze the factors affecting therapeutic outcome and to explore pathogenesis. Methods The adult patients with new-onset migraine who underwent transcatheter closure of ASD, accepted aspirin (100mg/d) for 12 months and Clopidogrel (75mg/d) for 3 month during the period from January 2013 to January 2017 in the Affiliated Yan'an Hospital of Kunming Medical University. The diagnosis and assessment of new-onset migraine were independently confirmed by neurologists, according to the criteria of the International Headache Society. Results 1006 patients were enrolled in the study. New-onset migraine occurred in 86 (8.55%) patients with a mean age of (39.76±12.94) years, in which migraine with aura and migraine without aura were respectively 67.44% (58), 32.56% (28). 59 (68.60%) patients were women. 53 (67.44%) patients had the diagnosis of new-onset migraine attacks within 1 week after the procedure. 83 patients accepted the therapy of aspirin and clopidogrel. The duration, frequency and intensity of migraine attacks were significantly and gradually improved from baseline level to respective levels of 1, 3, 6, 12 months after treatment (P＜0.05). The total curative rates were 59.04%, 72.23%, 86.75% and 74.70% respectively after the treatment of 1, 3, 6 and 12 months. The factor associated with clinical outcomes was the time of initial migraine attack. Conclusions The treatment of aspirin plus clopidogrel is safe and efficient for new- onset migraine in the adult patients who underwent transcatheter ASD closure, however the underlying mechanism needs to be further clarified. [ABSTRACT FROM AUTHOR]

Fish meal is rich in protein and fat and is the main animal-derived feed material. In the process of transportation and storage, the fish meal can be easily to deteriorate, resulting in the reduction of its nutritional components. This is not only endangers the normal reproduction and health of animals, but also poses certain potential safety hazards to the safety of animal-derived food and human health. It is necessary to carry out an effective detection for quality of animal-derived feed. In recent years, bionic olfaction technology has developed rapidly, it can quickly detect the quality of products, compared with near infrared spectroscopy and traditional physical and chemical index detection methods, it is faster and more accurate, but it is rarely used in the quality detection of fish meal. Therefore, in order to solve the above problems, a portable detection device on quality of fish meal has been developed in this paper. The hardware part of the device is mainly composed of a gas acquisition and transmission module, a control processing storage module, a data acquisition module, and a sensor array module, which the gas acquisition and transmission module includes a sample supplement gas cylinder, a sample gas generation chamber, an activated carbon purification bottle, two two-position two-way electromagnetic valves, a micro air pump, a gas flowmeter, a gas sampling chamber, and a one-way valve. In the software part, the strawberry pie is the core, qt creator graphical programming software across platforms was selected, and the real-time data acquisition display and storage part was mainly designed. The key component was the 10-bit ARPI600 data acquisition module. The device could basically realize the quality detection of fish meal, and the detection result was relatively accurate. In order to obtain the detection performance of the device, the test parameters of the device needed to be optimized. First, fresh fish meal was placed in a 35 ℃ thermostatic artificial climate box to make it decay gradually at the storage time. Fish meal of different levels was collected as the test sample during the storage process, and a total of six types of fish meal samples which was at different storage times were selected for the samples of the test. In this paper, it selected the factors that included gas flow, sampling time and cleaning time that had a great influence on the detection results, taking the dispersion ratio as an index, the optimal parameters were obtained through optimization analysis by using response surface method and design - expert software, and the feasibility and detection performance of the detection device for fish meal quality under the optimal parameters were verified. The test results showed that the gas flow rate, sampling time and cleaning time were all significant factors, and the primary and secondary order of factors was as follow: Cleaning time, sampling time and gas flow, and the interaction between them was significant. With the increase of gas flow and sampling time, the dispersion ratio tended to decrease first and then increase, and when the gas flow was in the range of 2-2.5 L/min and the sampling time was in the range of 35-45 s, the dispersion ratio was small. As the gas flow increased, the cleaning time decreased, while the dispersion ratio decreased first and then increased. When the gas flow rate was 2-2.5 L/ min and the cleaning time was in the range of 75-80 s, the dispersion ratio was small. As the sampling time increased, the cleaning time decreased, the dispersion ratio decreased first and then increased, and when the sampling time was in the range of 35-40 s and the cleaning time was in the range of 75-80 s, the dispersion ratio was small. In considering of all factors, the best parameters were 2.2 L/min of gas flow, 39 s of sampling time and 77 s of cleaning time. At this time, the dispersion ratio was the smallest, which was 0.579 9. Under the optimal parameters, fish meal samples with different storage time were tested, and the accuracy of different storage time discrimination was 89.4%, which could realize the detection of quality of fish meal and provide data reference and technical support for the subsequent research on the quality detection device. [ABSTRACT FROM AUTHOR]

To study the problems such as dramatic asymmetrical floor heave deformation of coal pillar roadway in fully mechanized caving face, difficulty in support and maintenance, theoretical analysis, numerical simulation and field test were adopted. The stress environment, deformation and failure characteristics of the coal pillar roadway in the fully mechanized caving face at Dananhu No. 1 coal mine in China were analyzed. The results demonstrate that the asymmetrical phenomenon of surrounding rock stress environment caused by mining is the intrinsic reason for the asymmetrical floor heave in the coal pillar roadway. It also leads to a non-uniform distribution of the plastic zone of floor in coal pillar roadway. The degree of asymmetrical floor heave has internal relations with the thickness of caving coal. When the thickness of caving coal is in the range of 5.9 m, the greater the parameters, such as the thickness of the caving coal, the breaking deflection angle of the strata of main roof, the principal stress ratio and the rotation angle of the principal stress direction in the stress environment of the roadway, the more obvious deformation of asymmetrical floor heave caused by plastic failure in floor. The maximum plastic failure depth position of the floor gradually moves toward the middle of the roadway as the rotation angle of the principal stress direction increases. It causes a different distribution of the maximum deformation position of floor heave. At the same time, the deformation of the floor heave of the coal pillar roadway is poorly controllable. It is not feasible to attempt to use high-strength support under the current technical condition. The control should mainly be adapted to the floor heave deformation. When the thickness of caving coal more than 5.9 m, the strata of main roof have the possibility of instability and being cut along the edge of the coal pillar, and the surrounding rock stress environment of the roadway tends to the original rock stress state. Consequently, the distribution range of the surrounding rock plastic zone is greatly reduced in the roadway. The deformation of floor heave is small. Based on the results above, the strategy of floor heave control based on adjusting the relationship of mining and driving, and optimizing the roadway floor heave treatment plan was proposed and good results were obtained. With the decrease of deformation of the floor heave, the working volume of floor heave was also greatly reduced. [ABSTRACT FROM AUTHOR]

BACKGROUND: With the development of surgical instruments and techniques, the unicompartmental knee arthroplasty has been accepted. However, there is still a lack of report on unicompartmental knee arthroplasty for treating medial compartmental knee osteoarthritis in young patients in China. OBJECTIVE: To evaluate the clinical curative effect of unicompartmental knee arthroplasty for medial compartmental knee osteoarthritis in young patients. METHODS: Sixteen patients less than 55 years of age with medial compartmental knee osteoarthritis were treated by unicompartmental knee arthroplasty with Oxford III system from June 2013 to June 2017 at Third Department of Orthopedics,Gansu Provincial Hospital. Curative effects were evaluated by Visual Analogue Scale, Hospital for Special Surgery score, range of motion and angle of femorotibial angle and hip-knee-ankle angle before and after surgery. RESULTS AND CONCLUSION: (1) Primary healing of incision was obtained in all patients, and no infection, fat embolism or fracture around the prosthesis occurred except one case of lower limb deep venous thrombosis. (2) All the patients were followed up for 6-36 months. (3) At the postoperative last follow-up, Visual Analogue Scale, Hospital for Special Surgery score, and range of motion of the knee joint were significantly improved compared with the baseline (P < 0.05). (4) The postoperative femorotibial angle and hip-knee-ankle angle were significantly improved compared with the baseline (P < 0.05). (5) These findings suggest that the unicompartmental knee arthroplasty for treating medial compartmental knee osteoarthritis in young patients has a good short-term effect. The moderate- and long-term efficacy needs long follow-up. [ABSTRACT FROM AUTHOR]

BACKGROUND: With the development of surgical instruments and techniques, the unicompartmental knee arthroplasty has been accepted. However, there is still a lack of report on unicompartmental knee arthroplasty for treating medial compartmental knee osteoarthritis in young patients in China. OBJECTIVE: To evaluate the clinical curative effect of unicompartmental knee arthroplasty for medial compartmental knee osteoarthritis in young patients. METHODS: Sixteen patients less than 55 years of age with medial compartmental knee osteoarthritis were treated by unicompartmental knee arthroplasty with Oxford III system from June 2013 to June 2017 at Third Department of Orthopedics,Gansu Provincial Hospital. Curative effects were evaluated by Visual Analogue Scale, Hospital for Special Surgery score, range of motion and angle of femorotibial angle and hip-knee-ankle angle before and after surgery. RESULTS AND CONCLUSION: (1) Primary healing of incision was obtained in all patients, and no infection, fat embolism or fracture around the prosthesis occurred except one case of lower limb deep venous thrombosis. (2) All the patients were followed up for 6-36 months. (3) At the postoperative last follow-up, Visual Analogue Scale, Hospital for Special Surgery score, and range of motion of the knee joint were significantly improved compared with the baseline (P < 0.05). (4) The postoperative femorotibial angle and hip-knee-ankle angle were significantly improved compared with the baseline (P < 0.05). (5) These findings suggest that the unicompartmental knee arthroplasty for treating medial compartmental knee osteoarthritis in young patients has a good short-term effect. The moderate- and long-term efficacy needs long follow-up. [ABSTRACT FROM AUTHOR]

With the development o£ the information society, the importance of network security has become increasingly prominent, and the accurate geographical location of network entities can help better implement network management. The existing classical network entity location method based on topology heuristic clustering adopts the clustering based on network structure to cluster network entities, does not consider the specific characteristics of the network topology, and leads to final results with big error. In order to solve this problem, we propose a target area network topology partitioning method based on the motifs. According to the high clustering characteristics o£ local nodes in the target network topology, we innovatively introduce the concept of "motif" and analyze the motif structure in the target network topology. Learning from the idea of the initial seed expansion in local community discovery methods for the complex network, we take the motif structure as the initial seed to expand, and divide the nodes closely connected with the motif in the topology into different sets. Finally we locate the node sets according to the landmark and the public IP geo-location database, and take the location of the set as the geographic location o£ the nodes within it so as to achieve the bulk positioning of network entities. Experiments based on the network topologies in Hong Kong and Taiwan show that compared with the classical HC-Based method and network node clustering method (NNC), the positioning accuracy of network entities of our method can be enhanced by about 25% and 16% respectively, and there are more network entities can be located in a batch way. [ABSTRACT FROM AUTHOR]

Objective To investigate the protective effect of recombinant adeno-associated virus (rAAV) mediated nerve growth factor (NGF) transfection by intramyocardial injection or subarachnoid injection in type 1 diabetic rats. Methods The whole experiment consists of two sub-experiments, experiment A:12 SPF grade male SD rats were randomly divided into control group (group control) and diabetes mellitus group (group DM, n=6). Group DM was given streptozotocin (STZ, 50 mg/kg, i.p) to induce type 1 diabetic rat model. The tail flick latencies were measured one day before STZ injection and 1, 2, 4, 6, 8 and 9 weeks after STZ injection in two groups of rats. The contents of NGF and calcitonin gene-related peptide (CGRP) were measured by ELISA method. Experiment B:24 SPF grade male SD rats were randomly divided into 4 group (n=6): diabetic heart transfection control group (group MC), diabetic heart transfection group (group ME), diabetic spinal cord transfection control group (group SC) and diabetic spinal cord transfection group (group SE). The 2×2 factorial design was used in this study. The diabetic rat model was established by giving STZ (50 mg/kg, i.p) in four groups of rats. At the end of 4 weeks after injection of STZ, rats in group MC and ME were transfected with rAAV-green florescent protein (GFP) and rAAV-NGF-GFP at a titer of 0.8×1013 virus genomes (μg/L) by intramyocardial injection at 100 μL. Rats in group SC and SE were transfected with rAAV-GFP and rAAV-NGF-GFP at a titer of 0.8 × 1012 virus genomes (μg / L) by subarachnoid injection at 25 μL. After 5 weeks, the cardiac function indexes were measured in groups of rats. The expressions of GFP in myocardium, spinal cord and dorsal root ganglion of T1-T5 were observed by fluorescence microscope, and the contents of NGF and CGRP were measured by ELISA method. Results In experiment A, after 4 weeks, the tail flick latency was significantly delayed in group DM compared with that of group control (P＜0.05). The NGF and CGRP protein in myocardium were significantly decreased in group DM compared with those of group control (P＜0.05). In experiment B, both rAAV-NGF-GFP and myocardial tissue as transfection pathway significantly improved the cardiac function of rats (P＜0.05), and up-regulated the contents of NGF and CGRP protein in myocardial tissue (P＜0.05). There was an interaction between transfectants and transfection pathways during the experiment. Combination of both can improve cardiac function and up-regulate NGF and CGRP in myocardium (P＜0.05). The myocardial point injection of rAAV-NGFGFP can significantly improve the cardiac function of rats (P＜0.01), and up-regulate the contents of NGF and CGRP protein in myocardial tissue (P＜0.01). Conclusion Compared with subarachnoid injection, intramyocardial injection of rAAV-NGF can effectively increase the expression of NGF in myocardial tissue of diabetic rats and have a more effective cardioprotective effect. eng [ABSTRACT FROM AUTHOR]

Objective · To explore the effect of cerebellar vermal repetitive transcranial magnetic stimulation(rTMS) with theta burst stimulation(TBS paradigm on cognitive functions of patients with schizophrenia. Methods · Thirty-one schizophrenia patients were randomly assigned into study group(n=14) and control group(n=17). Patients in the study group received a combination of rTMS and drug therapy, and patients in the control group received a combination of sham rTMS and drug therapy. The intervention lasted for 2 weeks, a total of 10 times. To evaluate the effect of rTMS on cognitive functions, MATRICS consensus cognitive battery(MCCB) was used to assess at baseline, after the intervention, 12 weeks and 24 weeks after the end of the intervention. Results · At baseline, there were no significant differences between two groups in the scores of each factor and the overall composite scores(P>0.05). After the intervention, repeated measures analysis of variance showed that the main effect of time was statistically significant in the scores of social cognitive(F=3.901, P<0.05). However, the main effect of group and the interaction between time and group showed no significance in the scores of the factor(P>0.05). Moreover, in the scores of speed of processing, attention/vigilance, working memory, verbal learning, visual learning, reasoning and problem solving and the overall composite scores, the main effect of group/time and the interaction between time and group were not significant(P>0.05).Conclusion · Application of TBS rTMS to cerebellar vermal is not superior compared with sham rTMS in improving the above seven cognitive domains. [ABSTRACT FROM AUTHOR]

A group of cylindrical cement mortar specimens and concrete specimens were designed and manufactured, and the average moisture transport coefficients of these specimens were obtained through moisture transport experiments. The value of β, which was introduced as the ratio between the moisture transport coefficients of interfacial transition zone (ITZ) and mortar matrix, was acquired by the parallel transport model of concrete specimens. Furthermore, influences of the water-cement ratio and sand-cement ratio of mortar matrix, the material and surface roughness of coarse aggregates on the value of β were studied. As the results indicate, the value of β ranges from 90 to 175. When the granitic coarse aggregate surface is original, the value of β is larger than that when the surface is sandblasted, and β remains about the same when water-cement ratio changes, however decreases with the increment of sand-cement ratio of mortar matrix. Furthermore, when the cross section type and mix proportion of cement mortar are uniformed, the value of β depends on both surface roughness and material type of coarse aggregate. [ABSTRACT FROM AUTHOR]

The limit state function for corroded reinforced concrete beams was established, in which the non-uniformity coefficient of corrosion was introduced to consider the non-uniformity of corrosion and randomness of steel bars. Then, according to the principle of conservation of probability, the generalized probability density evolution equation of the limit state function for corroded reinforced concrete beams was derived. Meanwhile,the finite difference method of TVD (total variation diminishing) difference scheme was recommended. Next, the time-dependent reliability calculation method based on the probability density evolution theory was proposed for corroded reinforced concrete beams through ‘absorption boundary conditions’. Further, the generalized probability density evolution equation and the calculation results of time-varying reliability were shown by taking three corroded reinforced concrete beams as examples. Moreover, the accuracies of the probability density evolution and the time-dependent reliability calculation were investigated through one million-scale of Monte Carlo simulation and the two-norm error index. The comparison results of the second-order moment information and the reliability show that the probability density evolution method can accurately capture the probability density evolution information of corroded reinforced concrete beams during its service period with a relative low calculation cost, and then accurately predict the time-varying reliability. [ABSTRACT FROM AUTHOR]

BACKGROUND: As the rate of re-fracture of the rotator is very high, the rotator cuff patch is designed to enhance the healing of the injured rotator cuff. Therefore, the preparation of temporary rotator cuff patch with good biocompatibility, biodegradability and biomechanical properties has become a committed step. OBJECTIVE: To prepare a temporary rotator cuff patch with different ratio of silk fibroin (SF)/polycaprolactone (PCL) using electrospinning technique, and to evaluate its physiochemical properties and biocompatibility. METHODS: Temporary rotator cuff patches with SF/PCL at different mass ratios of 4:0, 3:1, 1:1, 1:3, 0:4 were prepared using the electrospinning technique. Microstructure, contact angle, degradation properties and composition of the patches were characterized. Temporary rotator cuff patches with the mass ratios of 3:1, 1:1, 1:3, and 0:4 were implanted subcutaneously into the back of Sprague-Dawely rats. After 2 and 4 weeks of implantation, the patch material was taken for hematoxylin-eosin staining. RESULTS AND CONCLUSION: (1) The temporary rotator cuff patches prepared from the mixed material improved the diameter, aperture number, and pore diameter of the pure PCL patch as well as the surface droplets caused by the high brittleness of the pure SF patch. (2) The temporary rotator cuff patches prepared by mixed materials improved hydrophilicity as compared with the SF patch and improved hydrophobicity as compared with the simple PCL patch. (3) In the temporary rotator cuff patch prepared by mixed materials, no chemical reaction occurred between the two materials. (4) With the exception of the temporary rotator cuff patch with a mass ratio of 0:4, the rest patches showed an increase in quality after immersed in PBS for 4 weeks. (5) Two weeks after subcutaneous implantation, a large number of cells were attached to the rotator cuff patch in each group, and capillaries formed in the neonatal tissues. The outer middle layer of the rotator cuff patch had a microstructure similar to the normal tendon tissue, while the middle layer of the pure PCL patch was the worst in microstructure. (6) Four weeks after subcutaneous implantation, the number of cells in the neonatal tissue of the patch significantly decreased, and the thickness of the outer neonatal tissue similar to the normal tendon tissue increased, while the pure PCL patch showed the worst function in the middle layer of the new tissue. To conclude, the results show that SF improves the potential of PCL patch to induce the cell differentiation into tendon cells. [ABSTRACT FROM AUTHOR]

We propose a multi-scale wide line detector based on isotropic non-linear filtering to overcome the shortcomings of the basic wide line detector. The basic detector extracts wide lines using a single-scale circular mask, as a result, it can only detect lines thinner than the mask radius and has poor detection performance at intersections of two wide lines. Different from that, the multi-scale wide line detector computes the response with several circular masks of different radii, and chooses the maximum response among the normalized detection results on different scales. Finally, post-steps such as thresholding and filtering are designed to remove interference and obtain the final results. Simulated and real images are adopted for performance test of the multi-scale wide line detector. Experimental results demonstrate that it outperforms the basic wide line detector and can effectively detect wide lines in images. [ABSTRACT FROM AUTHOR]

Objective To investigate the role of FOXP2 in human hepatocellular carcinoma （HCC） . Methods qRT- PCR was used to detect the expression of FOXP2 in hepatocellular carcinoma cells BEL7402， HepG2，Hep3b，Huh7 and SMMC7721. The hepatocellular carcinoma cells with low expression of FOXP2 were used for further study. CCK- 8 and Clonogenic assay were used to test the proliferation of the cells. Western blot was used to test the protein expression of cyclinB1/ CDK1， AKT and p- AKT．Re s ults In hepatocellular carcinoma cells BEL7402， HepG2， Hep3b， Huh7 and SMMC7721， HepG2 cells exhibited the lowest expression of FOXP2. FOXP2 level was significantly increased in HepG2 cells when HepG2 cells were transfected with FOXP2 plasmid．Compared with before transfection， the proliferation ability of cells was improved after transfection （P＜ 0.05） ．The expression of cyclinB1/CDK1 in HepG2 cells transfected with FOXP2 plasmid was downregulated. Conclusions FOXP2 can inhibit the proliferation of human hepatocellular carcinoma via regulating AKT signal pathway and can be used as a new target for treatment of hepatocellular carcinoma. [ABSTRACT FROM AUTHOR]

Objective To obtain vascular smooth muscle cells (VSMCs) from spiral modiolar artery of guinea pigs with a combination of two common cultural methods so as to provide materials for related experimental studies. Methods Spiral modiolar artery was separated from the cochlea of guinea pigs first and then digested with 1.25g/L of trypsin to be allowed to adhere. It was then purified by comprehensive application of natural purification and differential adherence ability. Finally, the cells were confirmed by morphological observation, immunofluorescence and immunocytochemistry analysis, as well as Western blot. Results 85% of the explants survived. The smooth muscle cells at passage 4 had a purity of over 95%. The cells were observed to present a typical “hill-and-valley” growth pattern under the microscope. Moreover, immunofluorescence, immunohistochemical staining and Western blot showed the specific and intense positive expression of α-SM-actin, a cell type-specific marker. Conclusion By this protocol, we can obtain a variety of highly purified vascular smooth muscle cells from the spiral modiolar artery of guinea pigs, providing an ideal experiment material for studies on the pathogenesis of diseases related to the circulation disturbance of the inner ear in vitro, as well as evaluation of drug's effect on vascular smooth muscle. [ABSTRACT FROM AUTHOR]

Objective To investigate the expressions of relative inflammation factors in different KOA stages and their relationship with the clinical symptoms. Methods From July 2014 to September 2017 in the Second Department of Orthopedics and Trauma of the Second People's Hospital of Yichang，we selected 120 cases diagnosed as KOA according to certain criteria and divided theminto four groups: I stage group （9 cases），II stage group （50 cases），III stage group （37cases）；IV stage group （24 cases） based on the relative staging method. When the patients were admitted，the lesion knee fluid were withdrawn and checked in the Department of Pathology. At the same time，we conducted the VAS scoring and WOMAC scoring on patients．Result TNF-a：Groups of stage I and stage II showed no difference（ ＞0.05）；compared with group of stage II，stage III was lower （ ＜0.05）； compared with group of stage III，stage IV was lower（ ＜0.01） . IL-1：Groups of stage I and stage II showed no difference（ ＞0.05）；compared with group of stage II，stage III was lower （ ＜0.05）；groups of stage III and IV had no difference（ ＞0.05） . IL-6：groups of stage I and II had no difference；compared with group of stage II，stage III was lower （ ＜0.05）；groups of stage III and IV had no difference（ ＞0.05） . CRP：groups of stage I and II had no difference（ ＞0.05）；compared with group of stage II，stage III was preeminently lower （ ＜ 0.01）；groups of stage II and IV had no difference（ ＞0.05）. VAS scoring showed that the groups of stage III and IV were lower and the groups of stage I and II were significantly higher （ ＜0.01）；WOMAC scorings were reverse. Conclusion The initiative and explosion of inflammation factor may be characteristic in different KOA acute phase and may effect the clinical symptoms. [ABSTRACT FROM AUTHOR]

Objective To investigate the mid-and-long term impact of minimally invasive lithotomy and cholecystectomy on the living status of patients with cholelithiasis. Methods From January 2008 to January 2013， 214 cases who had received minimally invasive surgery in cholecyst (either minimally invasive lithotomy or minimally invasive cholecystectomy) and complied with study eligibility were recruited in the study. They were divided into minimally invasive lithotomy group and minimally invasive cholecystectomy group with 107 cases in each group. All the surgeries were performed by an experienced senior chief physician. Follow-up in half a year， 3 years and 5 years after surgery was conducted to evaluate the complications of cholecyst， liver and pancreas in patients， as well as their LDQ digestive function between the two groups. Results There was no significant different in the complications of cholecyst， liver and pancreas in patients of two groups in 6 months after surgery(P>0.05) . while， the LDQ digestive function was worse in patients receiving minimally invasive lithotomy group than in minimally invasive cholecystectomy group 6 months after surgery(P<0.05) . In 3 years and 5 years after the surgery， evaluation on complications of cholecyst， liver and pancreas in both groups showed significant difference (P<0.05)， however， the evaluation of LDQ digestive function showed no difference between the two groups (P>0.05) . Conclusion From a long-term perspective， for patients with complex cholecystolithiasis， physicians may choose the minimally invasive cholecystectomy for better effects. [ABSTRACT FROM AUTHOR]

Objective To investigate the correlation between 25-hydroxy-vitamin D ［25-(OH)D］ and serum lipid profile, and its clinical application in thrombolysis in myocardial infarction (TIMI). Methods From Dec. 2015 to May 2016, 89 non-ST-segment elevation myocardial infarction (NSTEMI) patients in the Department of Emergency, Zhongshan Hospital were enrolled in this study, as well as 89 health people who received physical examination at the same time. The clinical information, the concentration of 25-(OH)D and serum lipid profile of total 178 people were collected. The difference concentration of 25-(OH)D in serum between NSTEMI patients and health people were evaluated. The correlation of 25-(OH)D with serum lipid profile were analyzed by multiple linear regression. Logistic regression analysis was used to identified independent factors of TIMI in the NSTEMI group. Results The concentration of 25-(OH)D was significantly decreased in the NSTEMI patients compared to the health controls. The 25-(OH)D negatively correlated with total cholesterol, and positively correlated with triglyceride. 25-(OH)D was the independent protective factor of TIMI. Conclusions As the independent protective factor of TIMI, 25-(OH)D could play a subsidiary role in classifying NSTEMI patients in TIMI. [ABSTRACT FROM AUTHOR]

In this paper, the advantages and disadvantages of the tensile testing of the mechanical properties of the monofilament fiber are analyzed. A kind monofilament fiber stretching device of simple and easy to operate,without the need for advance preparation,length of the drawn fiber can be adjusted,non-manual positioning and clamping,high efficiency of the test. After discussing the principle of mechanical properties of monofilament fiber, according to the size and performance characteristics of monofilament fiber, the use of guide rail and fader combination structure to achieve monofilament fiber positioning before stretching. The ring electromagnet provides the suction force between the chuck and the T-shaped slider,controls the magnitude of the current in the solenoid coil, adjusts the clamping force between the chuck and the slider,and obtains the effective clamping force of the desired monofilament fiber, to achieve monofilament fiber holding. On this basis, the design of monofilament fiber positioning,clamping,stretching,measuring part of the device. [ABSTRACT FROM AUTHOR]

Choledocholithiasis is a common and frequently occurring disease in biliary tract surgery. With the advent of the era of precision medicine, accurate diagnosis and treatment of choledocholithiasis is significant. As the "navigation" method for the accurate treatment of choledocholithiasis, various methods has its advantages and limitations to meet the requirements of the treatment. Therefore, this article reviews the advantages and limitations of various imaging diagnostic methods of choledocholithiasis, and puts forward the strategies of imaging diagnosis in the era of precision medicine in order to provide a reference for the accurate diagnosis of choledocholithiasis. [ABSTRACT FROM AUTHOR]