1. MSH2 is required for cell proliferation, cell cycle control and cell invasiveness in colorectal cancer cells.
- Author
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Shen, Kai, Ye, YingJiang, Jiang, KeWei, Liang, Bin, Yang, XiaoDong, and Wang, Shan
- Subjects
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HEREDITARY nonpolyposis colorectal cancer , *CELL proliferation , *CELL cycle regulation , *CANCER invasiveness , *CANCER cells , *RNA interference , *MSH2 gene - Abstract
We have investigated the role of MSH2, a mismatch repair gene in cell proliferation, cell cycle control and cell invasiveness in the SW480 human colorectal cancer cell line. RNAi-mediated inhibition of MSH2 expression was achieved using MSH2 shRNA lentiviral expression vectors. Effective knockdown of endogenous MSH2 expression was determined by real-time PCR analysis. The most efficient MSH2 knockdown vector was selected for subsequent studies using SW480 cells. Endogenous MSH2 mRNA levels decreased after lentiviral delivery of the MSH2-RNAi, indicating efficient silencing of MSH2 expression in SW480 cells. Cell proliferation, cell cycle progression and cell invasiveness were quantified by MTT assays, flow cytometry and transwell assays, respectively. RNAi-mediated inhibition of MSH2 expression in SW480 cells resulted in decreased cell proliferation, cell cycle arrest at the G0/G1 phase and decreased cell invasiveness. Taken together, these results provide evidence that MSH2 stimulates cell proliferation, promotes cell cycle progression and positively regulates cell invasiveness. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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