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MSH2 is required for cell proliferation, cell cycle control and cell invasiveness in colorectal cancer cells.
- Source :
-
Chinese Science Bulletin . Oct2012, Vol. 57 Issue 20, p2580-2585. 6p. - Publication Year :
- 2012
-
Abstract
- We have investigated the role of MSH2, a mismatch repair gene in cell proliferation, cell cycle control and cell invasiveness in the SW480 human colorectal cancer cell line. RNAi-mediated inhibition of MSH2 expression was achieved using MSH2 shRNA lentiviral expression vectors. Effective knockdown of endogenous MSH2 expression was determined by real-time PCR analysis. The most efficient MSH2 knockdown vector was selected for subsequent studies using SW480 cells. Endogenous MSH2 mRNA levels decreased after lentiviral delivery of the MSH2-RNAi, indicating efficient silencing of MSH2 expression in SW480 cells. Cell proliferation, cell cycle progression and cell invasiveness were quantified by MTT assays, flow cytometry and transwell assays, respectively. RNAi-mediated inhibition of MSH2 expression in SW480 cells resulted in decreased cell proliferation, cell cycle arrest at the G0/G1 phase and decreased cell invasiveness. Taken together, these results provide evidence that MSH2 stimulates cell proliferation, promotes cell cycle progression and positively regulates cell invasiveness. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10016538
- Volume :
- 57
- Issue :
- 20
- Database :
- Academic Search Index
- Journal :
- Chinese Science Bulletin
- Publication Type :
- Academic Journal
- Accession number :
- 77440815
- Full Text :
- https://doi.org/10.1007/s11434-012-5258-0