1. Preparation of a novel glucose oxidase-N-succinyl chitosan nanospheres and its antifungal mechanism of action against Colletotrichum gloeosporioides.
- Author
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Niu, Xiaodi, Lin, Li, Liu, Lu, and Wang, Hongsu
- Subjects
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COLLETOTRICHUM gloeosporioides , *ANTHRACNOSE , *CHITOSAN , *ENZYME stability , *GLUCOSE oxidase , *COMPUTATIONAL biology , *FUNGAL spores , *FRUIT ripening - Abstract
In this work, a new glucose oxidase-N-succinyl chitosan (GOD-NSCS) nanospheres was prepared through the immobilization of glucose oxidase (GOD) on N-succinyl chitosan (NSCS) nanospheres. Compared to the free GOD, GOD-NSCS nanospheres demonstrated the excellent anti- Colletotrichum gloeosporioides activity with the EC 50 values of 211.2 and 10.7 μg/mL against mycelial growth and spores germination. The computational biology analysis demonstrated that the substrate presented the similar binding free energy with GOD-NSCS nanospheres (−27.64 kcal/mol) compared with the free GOD (−24.04 kcal/mol), indicating that GOD-NSCS nanospheres had the same oxidation efficiency and produced more H 2 O 2. Moreover, the enzyme activity stability of GOD-NSCS nanospheres could be prolonged to 10 d. The cell membrane was destructed by the treatment of H 2 O 2 produced by GOD, leading to the cell death. In vivo test, GOD-NSCS nanospheres treatment significantly prolonged the preservation period of mangoes 2-fold. Collectively, these results suggested that GOD-NSCS nanospheres suppresses anthracnose in postharvest mangoes by inhibiting the growth of C. gloeosporioides and might become a potential natural preservative for fruits and vegetables. • GOD-NSCS nanospheres was prepared. • The nanospheres had the better oxidation efficiency and produced more H 2 O 2. • GOD-NSCS nanospheres presented the excellent anti- C. gloeosporioides activity. • The shelf life of mangoes could be significantly prolonged by GOD-NSCS nanospheres. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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