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【Objective】The present paper aimed to investigate the important role of NAC gene in the growth and development of Cinnamomum camphora and its role in salt stress, and to clarify the response mechanism of C. camphora under salt stress. 【Method】The genome-wide NAC genes of C. camphora were identified and analyzed by bioinformatics to investigate the mode of response to abiotic stress in C. camphora. By identifying the members of NAC family of C. camphora,the paper aonducteda systematic analysis in terms of physicochemical properties, phylogenetic analysis, conserved motifs and gene structures, chromosomal localization and inter-species covariance, cis-acting element prediction and expression patterns.【Result】A total of 103 CcNAC genes were identified, the amino acid counts ranged from 137 to 1136, the isoelectric points from 4.54 to 10.00, the relative molecular masses from 15 644.40 to 129 859.29 u, and the mean hydrophobicity of all proteins was negative, indicating hydrophilicity. Subcellular localization was predicted to be in the nucleus and cytoplasm. A phylogenetic evolutionary tree was constructed with Arabidopsis thaliana NAC gene sequences, which classified CcNAC genes into 16 subfamilies. The NAM subfamily had the most members, with 17 CcNAC genes, while 25 CcNAC genes were not included. Conservative motif and gene structure analysis revealed that CcNAC genes were found to contain 2-8 introns and 10 motifs. Among these, motif3 appeared to be particularly stable in the genetic evolution of C. camphora genes, while motif9 and motif10 were distributed across the NAM subfamily. Chromosome localization and replication event analysis revealed that 103 genes were distributed across 12 chromosomes, with the highest number of genes（13） found in CcNAC genes distributed across chromosomes Chr02, with the lowest number of genes found on Chr10 and Chr11, which contained two genes each; Fragment duplication was the main mode of amplification for the CcNAC family. Multi-species co-linkage analysis revealed that CcNAC genes formed 162 pairs of co-linkage genes with CcNAC genes, indicating a closer relationship than with other species. The CcNAC genes were more closely related to the CcNAC genes and to those of other species than to any other. The CcNAC gene promoters contained several abiotic stress, growth and development, and hormone response elements. The results of the qRT-PCR showed that in leaves, the expression levels of the CcNAC058 and CcNAC092 genes were maximised under low salt stress. In roots, the expression of CcNAC007, CcNAC033, CcNAC058 and CcNAC092 genes increased with increasing salt concentration.【Conclusion】The NAC gene family may be involved in the process of salt stress response during the growth and development of C. camphora. The CcNAC092 and CcNAC058 genes exhibite significant responses in both leaves and roots under salt stress. It is hypothesised that CcNAC058 and CcNAC092 are the key genes contributing to the ability of the CcNAC to produce self-defensive behaviours under salt stress. [ABSTRACT FROM AUTHOR]

The crosstalk of phytohormones in the regulation of growth and development and the response of plants to environmental stresses is a cutting-edge research topic, especially in crop species. In this paper, we study the role and crosstalk between abscisic acid (ABA), ethylene (ET), and jasmonate (JA) in the control of germination and seedling growth in water or in standard nutrient solution and under salt stress (supplemented with 100–200 mM NaCl). The roles of ET and JA were studied using squash ET- and JA-deficient mutants aco1a and lox3a, respectively, while the crosstalk between ET, JA, and ABA was determined by comparing the expression of the key ABA, JA, and ET genes in wild-type (WT) and mutant genotypes under standard conditions and salt stress. Data showed that ET and JA are positive regulators of squash germination, a function that was found to be mediated by downregulating the ABA biosynthesis and signaling pathways. Under salt stress, aco1a germinated earlier than WT, while lox3a showed the same germination rate as WT, indicating that ET, but not JA, restricts squash germination under unfavorable salinity conditions, a function that was also mediated by upregulation of ABA. ET and JA were found to be negative regulators of plant growth during seedling establishment, although ET inhibits both the aerial part and the root, while JA inhibits only the root. Both aco1a and lox3a mutant roots showed increased tolerance to salt stress, a phenotype that was found to be mainly mediated by JA, although we cannot exclude that it is also mediated by ABA. [ABSTRACT FROM AUTHOR]

Rhizobacteria play a crucial role in plant growth and yield, stimulating primary production and improving stress resistance. Climate change has several consequences worldwide that affect arable land and agriculture. Studies on plant–soil–microorganism interactions to enhance plant productivity and/or resistance to abiotic stress may open new perspectives. This strategy aims to make agricultural‐relevant plant species able to complete their biological cycle in extreme soils with the help of inoculated or primed plant growth‐promoting rhizobacteria (PGPR).We provide an overview of the evolution of interest in PGPR research in the last 30 years through: (i) a quantitative search on the Scopus database; (ii) keyword frequencies and clustering analysis, and (iii) a keyword network and time‐gradient analysis.The review of scientific literature on PGPR highlighted an increase in publications in the last 15 years, and a specific time gradient on subtopics, such as abiotic stresses. The rise in PGPR as a keyword co‐occurring with salinity and drought stresses aligns with the growing number of papers from countries directly or partly affected by climate change.The study of PGPR, its features, and related applications will be a key challenge in the next decades, considering climate change effects on agriculture. The increased interest in PGPR leads to deeper knowledge focused specifically on researching agriculturally sustainable solutions for soils affected by salinity and drought. [ABSTRACT FROM AUTHOR]

Expansin is a cell wall relaxant protein that is common in plants and directly or indirectly participates in the whole process of plant root growth, development and morphogenesis. A well-developed root system helps plants to better absorb water and nutrients from the soil while effectively assisting them in resisting osmotic stress, such as salt stress. In this study, we observed and quantified the morphology of the roots of Arabidopsis overexpressing the TaEXPAs gene obtained by the research group in the early stage of development. We combined the bioinformatics analysis results relating to EXPA genes in five plants and identified TaEXPA7-B, a member of the EXPA family closely related to root development in winter wheat. Subcellular localization analysis of the TaEXPA7-B protein showed that it is located in the plant cell wall. In this study, the TaEXPA7-B gene was overexpressed in rice. The results showed that plant height, root length and the number of lateral roots of rice overexpressing the TaEXPA7-B gene were significantly higher than those of the wild type, and the expression of the TaEXPA7-B gene significantly promoted the growth of lateral root primordium and cortical cells. The plants were treated with 250 mM NaCl solution to simulate salt stress. The results showed that the accumulation of osmotic regulators, cell wall-related substances and the antioxidant enzyme activities of the overexpressed plants were higher than those of the wild type, and they had better salt tolerance. This paper discusses the effects of winter wheat expansins in plant root development and salt stress tolerance and provides a theoretical basis and relevant reference for screening high-quality expansin regulating root development and salt stress resistance in winter wheat and its application in crop molecular breeding. [ABSTRACT FROM AUTHOR]

Rice straw breakdown is sluggish, which makes agricultural waste management difficult, however pretreatment procedures and cellulolytic fungi can address this issue. Through ITS sequencing, Chaetomium globosum C1, Aspergillus sp. F2, and Ascomycota sp. SM2 were identified from diverse sources. Ascomycota sp. SM2 exhibited the highest carboxymethyl cellulase (CMCase) activity (0.86 IU/mL) and filter-paper cellulase (FPase) activity (1.054 FPU/mL), while Aspergillus sp. F2 showed the highest CMCase activity (0.185 IU/mL) after various pretreatments of rice straw. These fungi thrived across a wide pH range, with Ascomycota sp. SM2 from pH 4 to 9, Aspergillus sp. F2, and Chaetomium globosum C1 thriving in alkaline conditions (pH 9). FTIR spectroscopy revealed significant structural changes in rice straw after enzymatic hydrolysis and solid-state fermentation, indicating lignin, cellulose, and hemicellulose degradation. Soil amendments with pretreated rice straw, cow manure, biochar, and these fungi increased root growth and soil nutrient availability, even under severe salt stress (up to 9.3 dS/m). The study emphasizes the need for a better understanding of Ascomycota sp. degradation capabilities and proposes that using cellulolytic fungus and pretreatment rice straw into soil amendments could mitigate salt-related difficulties and improve nutrient availability in salty soils. [ABSTRACT FROM AUTHOR]

The SnRK (sucrose non-fermentation-related protein kinase) plays an important role in regulating various signals in plants. However, as an important bamboo shoot and wood species, the response mechanism of PheSnRK in Phyllostachys edulis to hormones, low energy and stress remains unclear. In this paper, we focused on the structure, expression, and response of SnRK to hormones and sugars. In this study, we identified 75 PheSnRK genes from the Moso bamboo genome, which can be divided into three groups according to the evolutionary relationship. Cis-element analysis has shown that the PheSnRK gene can respond to various hormones, light, and stress. The PheSnRK2.9 proteins were localized in the nucleus and cytoplasm. Transgenic experiments showed that overexpression of PheSnRK2.9 inhibited root development, the plants were salt-tolerant and exhibited slowed starch consumption in Arabidopsis in the dark. The results of yeast one-hybrid and dual luciferase assay showed that PheIAAs and PheNACs can regulate PheSnRK2.9 gene expression by binding to the promoter of PheSnRK2.9. This study provided a comprehensive understanding of PheSnRK genes of Moso bamboo, which provides valuable information for further research on energy regulation mechanism and stress response during the growth and development of Moso bamboo. [ABSTRACT FROM AUTHOR]

It is estimated that 40–70%, 80–90% and 50–90% of the conventional macronutrients N, P and K applied to the soil are lost, respectively, resulting in considerable loss of resources. Compared to conventional fertilizers, nanofertilizers have the advantages of controlled release, high nutrient utilization, low cost and relatively low environmental pollution due to their small size (1–100 nm) and high specific surface area. The application of nanofertilizers is an up-and-coming field of agricultural research and is an attractive and economical substitute for common fertilizers which can boost global food productivity sustainably. Foliar fertilization is a popular way to satisfy the needs of higher plants. Because of its small application dose, faster nutrient uptake than soil application and relatively less environmental pollution, foliar fertilization is more popular among plants. It can be seen that nanofertilizers and foliar fertilization are the hotspots of attention at present and that current research on the foliar application of nanofertilizers is not as extensive as that on soil application. Based on this background, this paper provides an overview of various applications of foliar spraying of nanofertilizers in agriculture, including applications in improving crop yield and quality as well as mitigating heavy metal stress, salt stress and drought stress. [ABSTRACT FROM AUTHOR]

Soil salinisation is a growing threat to global agriculture, reducing crop yields. Brassicaceae crops are vital vegetables and cash crops. Salt stress significantly affects the growth and development of Brassicaceae crops. A better understanding of the molecular and physiological mechanisms of salt tolerance is of theoretical and practical importance to improve Brassicaceae crop's salt tolerance and crop quality. Combined with previous research results, we discuss recent advances in research on salt stress response and salt tolerance in Brassicaceae crops. We summarised recent research progress on the physiological and molecular mechanisms of ionic homeostasis, antioxidant regulation, hormonal regulation and accumulation of osmotic-adjustment substances. We also discussed the molecular mechanism of Brassicaceae crop salt tolerant varieties from the perspective of differentially expressed genes, differentially expressed proteins and metabolites through transcriptome, proteome and metabonomic analysis methods. This paper summarises the molecular mechanisms in the perspective of differentially expressed genes, differentially expressed proteins, and metabolites through transcriptomic, proteome and metabolomics analysis. The review provides abundant data for accelerating the breeding of salt-tolerant Brassicaceae and laid a foundation for understanding the mechanism of salt tolerance of Brassicaceae crops and breeding salt-tolerance varieties. [ABSTRACT FROM AUTHOR]

[Objective] The paper aimed to study the effects of spraying different concentrations of CO donors on processed tomato seedlings under salt stress, and provided a theoretical basis for exploring the role of CO in areas such as improving salt tolerance in tomato. [Method] Processed tomato line KT-32 was used as the experimental material, and four treatments of exogenous CO donors (0, 0.05, 0.10, 0.15, 0.25 mmol/L) were set up by hydroponic culture with Hoagland nutrient solution culture (CK) and salt stress (100 mmol/L NaCl) + spraying, the root morphology, osmoregulatory substance content, reactive oxygen species (ROS), membrane lipid activity degree and cell membrane permeability of each treatment were measured. Combining analysis of variance and principal component analysis, the optimal concentration of exogenous CO donors under 100 mmol/L NaCl stress was analyzed. [Result]] Salt stress inhibited root growth of processed tomato seedlings, causing a decrease in total root length, root volume, and average root diameter, an increase in reactive oxygen species content in leaves, degradation of chlorophyll and proteins, and damaged to cell membrane structure and activation of protective enzyme systems. The injury of salt stress on processed tomatoes was alleviated when CO donors were exogenously applied, with the best effect at a concentration of 15 µmol/L (H3), and at 9 days of salt treatment, total root length, root projected area, root surface area, root mean diameter, and root volume were increased by 24. 22%, 38.16%, 38. 16%, 24.51%, 24.51%, and 53.97%, chlorophyll a, chlorophyll b, and total chlorophyll content increased by 29. 04%, 34.73%, and 30.75%, respectively, and osmoregulatory substance proline content, soluble sugar content, and relative water content of leaves increased by 40.68%, 55.20%, and 117.34%, respectively, and the rate of reactive oxygen species (ROS) O2- production and H2O2 content in leaves decreased by 47.34% and 20.91%, respectively; The activities of protective enzymes SOD, POD and APX increased by 36. 19%, 97.23% and 135.35%, respectively; MDA content and relative conductivity decreased by 57.82% and 19.35%, respectively; 21 indicators of root morphology and physiology of processed tomato seedlings were normalized by the principal component analysis showed that the overall score of H3 treatment was higher than that of other treatments subjected to salt stress, ranking second among all treatments at 2. 25. [Conclusion] Exogenous CO increases the antioxidant enzyme activity and osmoregulatory substances of processed tomato seedlings under salt stress, reduces membrane lipid peroxidation, increases photosynthetic pigment content, maintains the relative stability of the antioxidant enzyme system and reactive oxygen species in the internal and external membrane environment, and has a concentration effect, with the best alleviation effect at 15 µmol/L. [ABSTRACT FROM AUTHOR]

Emmenopterys henryi Oliv., a monotypic genus in family Rubiaceae with high scientific and application value, is currently endangered. Adaptability of E. henryi to environment is important for its cultivation, management, and application. In this paper, the effect of abscisic acid (ABA) on the photosynthesis of E. henryi under salt stress was discussed. Results showed that 0.3 % (m/v) NaCl led to reduction of net photosynthetic rate (PN), instantaneous carboxylation efficiency (ICE), and Rubisco activity but the increase of intercellular CO2 concentration (ci), which were remarkable relieved by ABA pretreatment. NaCl also induced reduction of the maximal efficiency of PS II photochemistry (Fv/Fm), the photochemical quenching (qP), the non-photochemical quenching (NPQ), the actual PS II efficiency [Y(II)], the regulated-energy dissipation [Y(NPQ)], whereas the increase of the unregulated energy dissipation [Y(NO)]. ABA application remarkably mitigated the depression of Fv/Fm, qP, Y(II) and the increase of Y(NO), but further promoted NPQ and Y(NPQ) in E. henryi due to high salinity. In addition, decrease of chlorophyll content, accumulation of MDA, and ion leakage in E. henryi due to high salinity were significantly alleviated by ABA pretreatment. Fluridone, an inhibitor of endogenous ABA synthesis, partially or completely reversed ABA mediated effects. These results suggested that ABA might improve the photosynthesis of E. henryi under salt stress via allocating more energy to photochemical reactions, or/and mitigating the decrease of Rubisco activity, or/and protecting photosynthetic apparatus by reinforcement of heat dissipation and alleviating oxidative damage. [ABSTRACT FROM AUTHOR]

Trehalose is a nonreducing disaccharide that is involved in the regulation of plant responses to a variety of environmental stresses. Trehalose 6-phosphate synthase (TPS) and trehalose 6-phosphate phosphatase (TPP) are two key enzymes in trehalose synthesis and they are widely distributed in higher plants. At present, TPS family genes have been systematically identified and analyzed in many plant species, but the TPP family genes have been rarely studied. In this study, ten TPS and six TPP genes in cannabis (Cannabis sativa L.) were identified at the genomic level. The phylogenetic tree of TPS and TPP family members in cannabis, Arabidopsis, and rice was constructed, and all the genes were divided into three subgroups: Class I, Class II, and Class III. The number of exons and motif types among Class I members was exactly the same, as were Class II members, but the gene structure and motif types of Class III members were slightly different. There were four pairs of CsTPSs and CsTPPs that had gene duplication, indicating that gene duplication events played an important role in the amplification of TPS and TPP families in cannabis. The results of expression analysis under abiotic stresses showed that 68.75 % of CsTPS and CsTPP genes were significantly induced by at least one abiotic stress. Among these genes, the expression of CsTPS1, CsTPS9, and CsTPPA was highest under at least one abiotic stress. These three genes may play a key role in abiotic stress responses. Most of the CsTPS and CsTPP genes that are closely located in the evolutionary tree have the same or similar functions. To our knowledge, this is the first paper that systematically reports the TPS and TPP gene families in cannabis. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to enrich the salt tolerance gene resources of Arundo donax and provide help for molecular breeding.【Method】The ‘LZ＿ No.1’ A. donax with consistent growth were treated with 0, 50, 100, 150 and 200 mmol/L NaCl salt solutions for 36 hours, and each treatment consisted of 3 replicates. Stems from the same site were collected for transcriptome sequencing and bioinformatics analysis. 5 differentially expressed genes were randomly selected for qRT-PCR to verify the expression pattern.【Result】De novo assembly was performed on the sequencing data after quality control, and a total of 971 309 transcripts were generated, with a GC content of 47.14%, and the N50 of Unigene contigs reached 1126 bp, indicating a good assembly effect. The 7305 differentially expressed genes identified were subjected to GO and KEGG enrichment analysis, and the results showed that stems of ‘LZ＿ No.1’ responded to salt stress mainly through nitrogen metabolism, ion transport, terpenoid synthesis, zeatin synthesis, linoleic acid metabolism, salicylic acid biosynthesis and osmotic regulator substances. The differentially expressed genes were screened, and a total of 104 core differentially expressed genes were obtained, of which 88315＿c0＿g1＿i1, 1200＿c2＿g1＿i1, 13330＿c0＿g1＿i8, 46571＿c0＿g1＿i7, 3434＿c0＿g1＿i11 and 713＿c0＿g1＿i32 genes with large differential multiples may play an important role in the response to the salt. The identification of transcription factors indicated that AP2/ERF-ERF family, MYB-related family and WRKY family were the most important gene families in salt response, and most of these three transcription factor family members were up-regulated in response to salt stress, indicating that they mainly worked by increasing the expression of genes. The detection results of the five differentially expressed genes by qRT-PCR were basically consistent with the RNA-seq results, which also demonstrated the reliability of the transcriptome data.【Conclusion】The study identifies important genes and transcription factors of A.donax in response to salt stress, which can lay a solid foundation for its future genetic improvement work. [ABSTRACT FROM AUTHOR]

In recent years, plant growth-promoting rhizobacteria (PGPR) have received increased attention due to their prospective use as biofertilizers for the enhancement of crop growth and yields. However, there is a growing need to identify new PGPR isolates with additional beneficial properties. In this paper, we describe the identification of a new strain of a non-sporulating Gram-positive bacterium isolated from the rhizosphere of potato plants, classified as Brevibacterium sediminis MG-1 based on whole-genome sequencing. The bacteria are aerobic; they grow in a pH range of 6.0–10.0 (optimum 6.0), and a temperature range of 20–37 °C (optimum 30 °C). At 96 h of cultivation, strain MG-1 synthesizes 28.65 µg/ml of indole-3-acetic acid (IAA) when 500 µg/ml of l-tryptophan is added. It is a producer of catechol-type siderophores and ACC deaminase (213 ± 12.34 ng/ml) and shows halotolerance. Treatment of pea, rye, and wheat seeds with a suspension of MG-1 strain cells resulted in the stimulation of stem and root biomass accumulation by 12–26% and 6–25% (P < 0.05), respectively. Treatment of seeds with bacteria in the presence of high salt concentration reduced the negative effects of salt stress on plant growth by 18–50%. The hypothetical gene lin, encoding the bacteriocin Linocin-M18, RIPP-like proteins, and polyketide synthase type III (T3PKS) loci, gene clusters responsible for iron acquisition and metabolism of siderophores, as well as gene clusters responsible for auxin biosynthesis, were identified in the B. sediminis MG-1 genome. Thus, the rhizosphere-associated strain B. sediminis MG-1 has growth-stimulating properties and can be useful for the treatment of plants grown on soils with high salinity. [ABSTRACT FROM AUTHOR]

Salt stress seriously affects plant growth and is a serious factor restricting the safe production of crops. High salt can cause disturbance of ions in plants, and ultimately affect the development, growth and yield of plants. It is very important to understand and improve the salt resistance mechanism of crops to ensure the safe production of crops. In this paper, the harm of salt stress on plants, sodium ion transport, and the response mechanism of plants to salt stress were reviewed. The aim is to provide some theoretical basis for promoting the study of the responding mechanisms of salt stress in plants. [ABSTRACT FROM AUTHOR]

Cotton is an important plant grown worldwide that used as a model organism in salt resistance studies. At present, the study of the molecular genetic basis of salt resistance, a comprehensive study of the mechanisms of resistance is important for the creation and use of salt-resistant varieties and forms. miRNAs belong to the class of endogenous small non-encoding RNAs that regulate gene expression at the transcription and posttranscription levels. Studies show that the study of molecular mechanisms regulated by miRNAs can offer new approaches to plant growth and productivity under various abiotic and biotic stress conditions. In the paper, the biogenesis of miRNAs and the role of miRNAs in the salt resistance reactions of cotton is considered. [ABSTRACT FROM AUTHOR]

Trehalose is a nonreducing disaccharide that is involved in the regulation of plant responses to a variety of environmental stresses. Trehalose 6-phosphate synthase (TPS) and trehalose 6-phosphate phosphatase (TPP) are two key enzymes in trehalose synthesis and they are widely distributed in higher plants. At present, TPS family genes have been systematically identified and analyzed in many plant species, but the TPP family genes have been rarely studied. In this study, ten TPS and six TPP genes in cannabis (Cannabis sativa L.) were identified at the genomic level. The phylogenetic tree of TPS and TPP family members in cannabis, Arabidopsis, and rice was constructed, and all the genes were divided into three subgroups: Class I, Class II, and Class III. The number of exons and motif types among Class I members was exactly the same, as were Class II members, but the gene structure and motif types of Class III members were slightly different. There were four pairs of CsTPSs and CsTPPs that had gene duplication, indicating that gene duplication events played an important role in the amplification of TPS and TPP families in cannabis. The results of expression analysis under abiotic stresses showed that 68.75 % of CsTPS and CsTPP genes were significantly induced by at least one abiotic stress. Among these genes, the expression of CsTPS1, CsTPS9, and CsTPPA was highest under at least one abiotic stress. These three genes may play a key role in abiotic stress responses. Most of the CsTPS and CsTPP genes that are closely located in the evolutionary tree have the same or similar functions. To our knowledge, this is the first paper that systematically reports the TPS and TPP gene families in cannabis. [ABSTRACT FROM AUTHOR]

[Display omitted] • Detection of catalase activity in tomato leaves cells is used by microscopic hyperspectral imaging technology. • The feature wavelengths are extracted by SPA, IVISSA, IRFJ, GAPLSR, CARS methods. • A model for micro-cell is built through model migration of transfer learning method. • This study provides a new way for the innovation of Catalase activity nondestructive testing technology. Salt stress easily leads to oxidative stress and promotes the catalase (CAT) response in tomato leaves. For the changes in catalase activity in leaf subcells, there is a need for a visual in situ detection method and mechanism analysis. This paper, taking catalase in leaf subcells under salt stress as the starting point, describes the use of microscopic hyperspectral imaging technology to dynamically detect and study catalase activity from a microscopic perspective, and lay the theoretical foundation for exploring the detection limit of catalase activity under salt stress. In this study, a total of 298 microscopic images were obtained under different concentrations of salt stress (0 g/L, 1 g/L, 2 g/L, 3 g/L) in the spectral range of 400–1000 nm. With the increase in salt solution concentration and the advancement of the growth period, the CAT activity value increased. Regions of interest were extracted according to the reflectance of the samples, and the model was established by combining CAT activity. The characteristic wavelength was extracted by five methods (SPA, IVISSA, IRFJ, GAPLSR and CARS), and four models (PLSR, PCR, CNN and LSSVM) were established according to the characteristic wavelengths. The results show that the random sampling (RS) method was better for the selection samples of the correction set and prediction set. Raw wavelengths are optimized as the pretreatment method. The partial least-squares regression model based on the IRFJ method is the best, and the coefficient of correlation (R p) and root mean square error of the prediction set (RMSEP) are 0.81 and 58.03 U/g, respectively. According to the ratio of microarea area to the area of the macroscopic tomato leaf slice, the R p and RMSEP of the prediction model for the detection of microarea cells are 0.71 and 23.00 U/g, respectively. Finally, the optimal model was used for quantitative visualization analysis of CAT activity in tomato leaves, and the distribution of CAT activity was consistent with its color trend. The results show that it is feasible to detect the CAT activity in tomato leaves by microhyperspectral imaging combined with stoichiometry. [ABSTRACT FROM AUTHOR]

The primary objective of our study was to mix thermally remediated oil-bearing drilling waste (TRODW) with farmland soil during wheat planting and explore the response of microbial phospholipid fatty acid (PLFA) communities as well as the feasibility of returning TRODW to farmland. Based on environmental protection requirements and the dynamic response of wheat soil, this paper not only provides a method combining multiple models for mutual verification but also provides valuable and exploratory information for the remediation and reuse of oily solid waste. Our research found that salt damage mainly originated from sodium ions and chloride ions that inhibited the development of microbial PLFA communities in the treated soils at the initial stage. When salt damage declined, TRODW improved the levels of phosphorus, potassium, hydrolysable nitrogen and soil moisture, increasing the soil health status and promoting the development of microbial PLFA communities even when the addition ratio reached 10%. Moreover, the influences of petroleum hydrocarbons and heavy metal ions on microbial PLFA community development were not significant. Therefore, when salt damage is controlled effectively and the oil content in TRODW is no more than 3‰, it is potentially feasible to return TRODW to farmland. [Display omitted] • Na, Cl−, EC and pH in TRODW significantly affect the development of soil PLFAs. • TRODW promoted the development of soil PLFAs when salt damage was controlled well. • Salinization in TRODW, instead of heavy metals, is the main reason for soil damage. • TRODW can improve soil nutrients and moisture when salt damage is controlled well. [ABSTRACT FROM AUTHOR]

Phytolectin PP2 mainly exists in the phloem, and studies have shown that PP2 has the function of stress defense, especially against biotic harm brought from pests and diseases. According to the proteomic analysis of Cucumis sativus phloem sap in our previous research, a detected increased expression of PP2-A1 in cucumber in response to salinity indicated that PP2-A1 might be relevant to plant salt tolerance. In this paper, the expression of CsPP2-A1 assayed by qRT-PCR proved that the CsPP2-A1 could be induced in cucumber by a variety of biotic, abiotic, or phytohormones stresses. Moreover, the CsPP2-A1 gene was cloned and transferred into Arabidopsis and cucumber separately. The obtained CsPP2-A1 overexpressing (OE) Arabidopsis and cucumber phenotypically exhibited greater resistance to saline stress than the WT plants. Furthermore, the physiological experiments showed that the CsPP2-A1 -OE plants possessed lower MDA contents and higher antioxidant enzyme activities (CAT, POD, and SOD) than wild-type (WT) plants. Meanwhile, transcriptome sequencing assay found that 2256 genes were differently expressed in CsPP2-A1 -OE cucumber. Most of the differentially expressed genes (DEGs) were mainly enriched in the MAPK pathway, ABA, and JA signaling pathway and participated in stress adaptation and response. All the evidence indicated that CsPP2-A1 was engaged in plant salinity adaption and the salt tolerance enhancement might involve in the ABA-JA signaling pathway and the antioxidant system. • CsPP2-A1 could be induced biotic, abiotic or phytohormones stresses. • CsPP2-A1 enhanced salt tolerance in both Arabidopsis and cucumber. • CsPP2-A1 participated the ABA-JA signal transduction pathway and the antioxidant system. [ABSTRACT FROM AUTHOR]

Heterosis or hybrid vigor is a phenomenon that a heterozygote is superior to two parental lines in one or more traits, or even responses against stresses. However, related molecular mechanism is still not fully elucidated. In this paper, we investigated whether hydrogen sulfide (H 2 S), a universal signaling molecule in both mammals and plants, participates in heterosis. When upon salinity stress, two parents of Brassica napus (NJ4375 and MB1894) were more sensitive to salinity compared to the hybrid (F1, NJ4375×MB1894), evaluated by the improving effects on the reduction in shoot length, root length, fresh weight of root parts, and chlorophyll content. It was further observed that the increased endogenous H 2 S synthesis was more pronounced in the hybrid than those of two parental lines when stressed with salinity, suggesting the important roles of endogenous H 2 S. The removal of endogenous H 2 S by its biosynthesis inhibitor (PAG) and scavenger (HT) could differentially abolish the salinity tolerance observed in the hybrid. Subsequent experiments showed that the hybrid could maintain ion homeostasis by regulating the sodium and potassium transporters, which was confirmed by modulating NHX1 , SOS2 , AKT1 and HAK5 transcripts. Proline content was also significantly increased and lipid peroxidation was obviously alleviated in the hybrid plants compared to those in both NJ4375 and MB1894. Above responses in hybrid were sensitive to the inhibition of endogenous H 2 S synthesis or its scavenging. Overall, our results clearly provided the evidence that salt-tolerant heterosis of hybrid might be closely associated with endogenous H 2 S signaling. • Endogenous H 2 S production was intensified in the hybrids upon salinity stress. • Salt-tolerant heterosis of hybrid might be associated with H 2 S. • H 2 S-mediated ion balance functions in heterosis control of salinity tolerance. [ABSTRACT FROM AUTHOR]