1. A surface-enhanced Raman spectroscopy substrate for highly sensitive label-free immunoassay.
- Author
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Zhang, Ming-Liang, Yi, Chang-Qing, Fan, Xia, Peng, Kui-Qing, Wong, Ning-Bew, Yang, Meng-Su, Zhang, Rui-Qin, and Lee, Shuit-Tong
- Subjects
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RAMAN spectroscopy , *IMMUNOASSAY , *NANOWIRES , *SILICON , *SILVER , *NANOPARTICLES - Abstract
Large-scale uniform silicon nanowires (SiNWs) array was fabricated by chemical etching on n-Si(111) wafer. Silver nanoparticles (AgNPs) were loaded on their surfaces. The AgNPs on SiNWs (AgNPs@SiNWs) array exhibit strong surface-enhanced Raman effect. On the substrate surfaces, characteristic Raman signals are generated with trace amount of mouse immunoglobulin G (mIgG), goat-anti-mouse immunoglobulin G (gamIgG), and immune complexes formed from 4 ng each of mIgG and gamIgG. The shifted positions and changed intensities in Raman bands indicate the occurrence of immunoreactions. This AgNPs@SiNWs array is a unique substrate for surface-enhanced Raman spectroscopy to show the immune reagents and immunoreactions at higher sensitivity. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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