1. 甲基苯丙胺对大鼠海马5-HT、IL-6及NF-κB 表达的影响.
- Author
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杨金凤, 蒙国懿, 黄兆奎, 洪仕君, 李利华, 邢豫明, and 赵永娜
- Abstract
Objective To study the effects of methamphetamine on the expression of 5- Hydroxytryptophan (5-HT), interleukin-6 (IL-6) and nuclear fact kappa B (NF-κB) in rats' hippocampus. Methods Thirty SD rats were randomly allocated into normal control group,METH1d group,METH3d group, METH5d group and METH7d group,with 6 rats in each group.Intraperitoneal injections of METH [15mg/(kg·time)] were conducted at 9:00am and 21:00pm every day.After injection,scoring was performed in combination with observation on their stereotyped behavior. We established different models for rats suffering from 1-day,3-day,5-day,and 7-day METH acute poisoning. After 24 hours of the last intraperitoneal injections of METH,hippocampal tissues were isolated.Enzyme linked immunoorbent assay (ELISA) was applied to detect the expression level of 5-HT and IL-6 in rats hippocampus. Western blot was used to detect the expression level of NF-κBp65.Results Compared with the control group,the expression of 5-HT was reduced in different METH groups,and the decrease was statistically significant in METH5d and METH7d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group,the expression of IL-6 was increased in different METH groups, and the increase was statistically significant in METH5d and METH7d groups (with P<0.05 and P<0.01 respectively) . Compared with the control group, the expression of NF-κB was increased in different groups, and the increase was statistically significant only in METH7d group (P<0.01) . Conclusion METH administration could result in a significant reduce of 5-HT expression and increases of IL-6 and NF-κB expressions in rats hippocampus. [ABSTRACT FROM AUTHOR]
- Published
- 2018