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Succinate dehydrogenase flavocoenzyme (‘SD-flavin’), previously shown to be an 8α-substituted riboflavin derivative containing a tertiary nitrogen homoconjugated to the flavin nucleus, was subjected to further hydrolysis and to reduction under acid conditions. Both conditions resulted in the liberation of 1 mole of histidine per mole of flavin. This proves histidine to be the covalent link between flavin and peptide backbone in succinate dehydrogenase and imidazole to be the tertiary nitrogen function homoconjugated to the flavin. 8α-Histidyl-riboflavin has been synthesized starting from riboflavin chemically and shown to be completely identical with the natural product in optical, ESR and NMR spectra, pH-fluorescence curve and behavior on thin-layer and paper chromatography, as well as paper electrophoresis. [This equation cannot be represented into ASCII Text] 3. Both the natural compound isolated by acid hydrolysis of flavin peptide and the synthetic one contain two isomers, which may be separated by high voltage electrophoresis. The isomers appear to be the N(1)- and N(3)-imidazole substituted compounds. Digestion of the flavin peptide with aminopeptidase M yields only one isomer but on treatment with 6-N HCl this is gradually converted to a mixture of the two isomers. The absolute assignment of the natural isomer is suggested as 8α-[N(3)-histidyl]-riboflavin on the basis of imidazole quaternization with CH3I, reductive cleavage of the flavin-imidazole bond and identification of the methyl-histidine liberated as 1-methyl-histidine. [ABSTRACT FROM AUTHOR]

The article presents a discussion of Gardner Patterson's research paper. The reviewer presents comments on terminology and the other is a matter of emphasis. The point of terminology has to do with the concept of mandatory saving. This type of saving means a reduction in consumption forced on people by an increase in prices. The author states that it is important to note how much additional money an economy is able to absorb in deciding how much deficit financing can be undertaken without risking inflation.

1. A reasonably reproducible method has been developed for separating lysolecithin, lecithin,, sphingomyelin, and phosphatidylethanolamine, using glass fiber filter paper impregnated with silicic acid as the chromatographic medium and 1:1 methanol:ethyl ether as the developing solvent. 2. Hot sulfuric acid was used to locate the chromatographed compounds on the chromatogram. The advantages and disadvantages of this reagent and the other spot test reagents used were discussed. 3. No separation between the unsaturated and saturated counterparts of lysolecithin or lecithin was obtained. [ABSTRACT FROM AUTHOR]

This article highlights the 5th International Criminological Congress of the International Society for Criminology to be held in Montreal, Quebec, from August 29 to September 3, 1965.