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[Objective] The present paper aimed to clarify the expression pattern and molecular regulation mechanism of genes related to sugar, lipid and protein metabolism in maize ear leaves under different fertilization in field conditions. [ Method] Maize cultivar 'Luyu 16' was used as the material and five treatments were set up: No fertilizer (CK), nitrogen fertilizer ( N), N + P fertilizer (NP), N + K fertilizer ( NK) and N + P + K fertilizer ( NPK). At grain filling stage, the upper part of ear leaves was harvested for transcriptome analysis using RNA-Seq techniques. The differentially expressed genes ( DEGs) under different fertilizer treatments were analyzed by Hisat2 software. [Result]GO(Gene ontology)analysis showed that DEGs related to sugar, lipid and protein metabolism were enriched in Biological process ( BP), Cell components( CC) and Molecular function( MF). Although the enriched items were the same, the number of DEGs varied under different combinations. KEGG ( Kyoto encyclopedia of genes and genomes) analysis exhibited that more DEGs were enriched in carbon metabolism, starch and sucrose metabolism, and amino acid synthesis pathways. The total number of DEGs with both I log2 FC I > 1 and P < 0. 05 involved in sugar, lipid and protein metabolism under different fertilizer treatments was 82, 29 and 84, respectively. Among them, the total number of DE Gs with I log2 FC I > 2 was 6, 24 and 17. The number of DE Gs showed the maximum in comparisons of NPvsCK and NPKvsCK but the minimum in NvsCK comparison. Further analysis of DEGs with I log2 FC I showed that these DEGs had significant differences in the expression level and expression patterns. [ Conclusion] The combined of nitrogen, potassium and phosphorus fertilizer significantly affected the transcription levels of genes related to sugar, lipid and protein amino acids metabolism in maize ear leaves. Some key genes participating in the three organic substances' metabolism were identified. The results provided molecular basis for improving the photosynthetic efficiency of maize leaves by rational fertilization in the future. [ABSTRACT FROM AUTHOR]

The period of cultivation of large-diameter timber stands is affected by the relatively slow initial growth of Picea species. The combination of fertilization and nurturing measures can promote the growth of tree height and ground diameter. In this paper, 12 fixed plots of 10m×10m were randomly set up in a larch understory with 10 a juvenile Picea koraiensis plantations as the study subjects. Fertilizer types were selected as N (N - urea), NP (NP - urea+superphosphate), NPK (NPK - urea+superphosphate+potassium phosphate). Two types of fertilization time were set: (1) pre-middle-late growing seasons fertilization: fertilizer was applied per year at the beginning (mid-May), middle (mid-July), and late (mid-August) of the growing season, with fertilization repeated for four consecutive years. (2) Pre-middle-end growing seasons fertilization: fertilizer was applied per year at the beginning ( mid - May), middle (mid-July) and end (mid-September) of the growing season, with fertilization repeated for four consecutive years. Light-felling was set as follows, after one growing season of fertilization, accretion cutting the upper larch trees, and the stand density was 0. 6 after tending. By studying the effects of fertilization and light-felling on the growth of trees, the aim was to develop fertilization management techniques to promote the growth of juvenile P. koraiensis, so as to shorten the growth cycle of young plantation. The effect of applying NPK fertilizer on promoting the height and diameter growth of juvenile P. koraiensis was better than applying only a single fertilizer or a mixture of nitrogen and phosphorus fertilizers. The annual tree height and ground diameter growth were 41.11 % and 49.81 % higher in the treatments NPK fertilizer applied pre-mid-end growing seasons than those treated with NPK in the premid-late growing seasons. After light-felling, the annual growth of tree height between different years was not significantly different, but that of the ground diameter was significantly different. The annual growth of tree height and ground diameter after three growing seasons were not significantly different from that before light-felling. By combining fertilizer application in the pre-middle-late growing seasons with light-felling treatments, the annual growth of tree height and ground diameter were significantly different from the control group after two growing seasons, only the annual growth of ground diameter was significantly different from the control group after three growing seasons. After three growing seasons of nitrogen, phosphorus and potassium fertilizers (NPK2) combined with light-felling in the pre-mid-end of growing seasons, the annual growth of ground diameter was 153.16 % and 10.31 % higher than that of the control and pre-middle-late of growing seasons NPK fertilizers (NPK1) combined with light-felling. The combination of fertilization and light-felling treatments can increase the annual growth of P. koraiensis ground diameters, reaching a significant level at four consecutive years of fertilization. Fertilizer application at the pre-mid-end stages of the growing season is more effective than at pre-mid-late stages. The research provides a basis for solving the problem of slow growth of P. koraiensis plantation at a young age and shortening the cultivation period of large-diameter timber plantation of P. koraiensis. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to study the effect of physicochemical properties of rhizosphere soil on quality of Coptis deltoidea C. Y. Cheng et Hsiao and further to provide scientific basis for ecological planting of C. deltoidea. [Method] Eighteen soil physicochemical indexes and six alkaloids of C. deltoidea were determined by soil agrochemical analysis and high performance liquid chromatography (HPLC) . Meanwhile, correlation analysis and redundancy analysis were used to reveal the relationship between physico-chemical properties of soil and the accumulation of alkaloids in C. deltoidea. [Result] The total contents of 6 alkaloids in taproots of C. deltoidea that grew for two to five years old were 3. 716%, 5. 720%, 8. 552% and 8. 866%, respectively, and the contents of berberine were 1. 779%, 3. 620%, 5. 469% and 5. 706%, respectively, which indicated that the four-year-old C. deltoidea had met the harvesting standard. The correlation analysis of alkaloids and soil factors in the taproot of C. deltoidea showed that total amount of 6 alkaloids and berberine content were significantly positively correlated with available potassium (P＜0. 05), and significantly negatively correlated with nitrate nitrogen and sodium (P＜0. 01) . And a significant negative correlation was also seen between sodium and rhizobine (P＜0. 01) . There was significant negative correlation between coptidine and copper (P＜0. 05), and extremely significant negative correlation between coptidine and sodium (P ＜0. 01) . Finally, according to the redundancy analysis of alkaloids in taproot and soil factors, the contents of available phosphorus, available potassium and sodium in soil were the dominant factors affecting the accumulation of alkaloids in C. deltoidea, while ammonium nitrogen and pH were the more important factors. [Conclusion] In the process of planting C. deltoidea, high-quality soil environmental conditions can be selected from the planting source, that is, the soil with high nitrogen and potassium, low phosphorus and sodium and acidic pH can be selected. Moreover, the alkaloid content of C. deltoidea can be regulated by adding potassium fertilizer and nitrogen fertilizer, controlling the application amount of phosphate fertilizer and reducing the content of sodium in the soil. [ABSTRACT FROM AUTHOR]

【Objective】In order to provide a theoretical basis for the increase of yield and output value of tea and the nutrient absorption of big-leaf tea species in modern tea garden,this paper studied the effects of optimized fertilization technology on the quality of tea in modern tea garden of Pu’er.【Method】In this paper,‘Yunkang14’,an excellent tea variety,was used as the test material,and the plot experiment was carried out with treatment 1(no fertilization,CK),treatment 2(conventional fertilization),and treatment 3(Formula fertilization) and treatment 4(Optimized formula fertilization).The treatment 3 and treatment 4 had the same amount of fertilizer applied,but different fertilization methods.【Result】The accumulation of nitrogen,phosphorus and potassium in the new shoots of tea tree in treatment 4 were significantly higher than those in other treatments.The contents of phosphorus and potassium in tea shoots were significantly higher than other treatments; the agronomic efficiency of treatment 4 was 9.58 kg/kg for nitrogen fertilizer,22.99 kg/kg for phosphate fertilizer and 28.74 kg/kg for potash fertilizer,respectively,and their partial productivity was 23.14,55.54 and 69.42 kg/kg respectively.Compared with treatment 2 and treatment 3,the 100-bud weight of treatment 4 increased by 12.74 % and 2.06 %,the germination density increased by 10.84 % and 1.14 %,the fresh leaf yield increased by 15.71 % and 3.98 %,and the output value increased by 28 135 and 3224 yuan/hm2,respectively.【Conclusion】In the field experiment,the optimized fertilization technology can significantly improve the tea yield,output value and nutrient uptake of Pu’er modern tea garden.It is suggested to promote the optimized fertilization technology in Pu’er modern tea garden tea production [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to study the optimum fertilizer on tobacco cultivation, which would provide a theoretical basis for the reasonable fertilizer application of tobacco leaves in Liangshanyi autonomous prefecture. [Method] Taken Yunyan 87 as tested materi als, the effect of different fertilizer ratios of nitrogen ( N), phosphorus ( P) and potassium ( K) on the leaf biomass, quality components and Cd accumulation of Nicotiana tabacum were studied by pot assay. [ Result]The fertilizer application could not only promote the growth of to bacco and increase the leaf biomass, but also had a positive effect on the accumulation of quality components and the coordination of qnalitative proportion. The contribution efficiency of nitrogen, phosphorus and potassium to plant height and leaf biomass of tobacco was N > P > K. Potassium fertilizer played an important role in the accumulation of potassium in tobacco leaves. The contents of nicotine and total nitrogen were significantly increased, and the coordinated proportion of qnality components was presented under the high level of nitrogen fertilizer. Cd content was an important index to evaluate the qnality and safety of tobacco leaves. The content of Cd in tobacco leaves was in the order of lower leaves > middle leaves > upper leaves. Meanwhile, the abilities of Cd absorption and accumulation of tobacco plants to soil were sig nificantly improved with the high level of combined nitrogen and phosphorus fertilizer, nd storage in tobacco leaves, however, there was no notable influence for potassium fertilizer. [ Conclusion] In summary, the best fertilizer recommended by this pot assay was the treatment of N212K2,when the ration ofN: p: K was 1:0.45:2. 06, average plant height and leaf biomass were 79. 2 cm and 55. 2 g; With 3. 22%,3. 05%,3. 76%,25. 89% and 27. 74% for the content of potassium, nicotine, total nitrogen, reducing sugar and total sugar, respectively, the ratio of sugar and nicotine and the ratio of sugar and nitrogen were 9. 10 and 7. 38, respectively; The Cd contents of lower leaves, middle leaves and upper leaves were 1. S7, 1. 37 and 1. 11 mg/kg, respectively, which was statistically lower the limit level of 3. 0 mg/kg prescribed by the China Tobacco Corporation. It was suggested that this fertilization scheme could be widely applied in various tobacco areas and similar producing areas of Liangshanyi autonomous prefecture. [ABSTRACT FROM AUTHOR]

(Objective] The purpose of this paper was to study the effects of buse and topdressing with different potassium-rich soil conditioners on tobacco production during the period of soil cultivation. [ Method ] In this study, wood peat derived potassium humate, mineral potash (hydrothermal method) and calcium magnesium phosphate fertilizer were used instead of KNOW, or K, SO, on the base, to study their effects on soil pH, available potassium, slow potassium, tobacco leaf quality and economic status of flue-cured tobacco. [ Result ] The results showed that base and topdressing different potassium-rich soil conditioners could effectively improve soil pH of tobacco root zone and keep it between 5.5 -6.5. The effect is as follows T5 > T3 > T4 > T2 > TI, among which wood peat derived potassium humate (base fertilizer) and calcium magnesium phosphorus fertilizer had the best effect; Topdressing wood peat derived potassium humate, mineral potash (hydrothermal meth od) and calcium magnesium phosphate fertilizer could significantly improve available potassium, slow potassium content of soil and the pro portion of middle, upper leaves'. Among them, woody peat derived potassium humate (basie fertilizer) and mineral potassium fertilizer (hydrothermal method) had the best application effect at 1:3. Compared with the control, the content of available potassium was increased by 60.95 % (0 - 10 cm),77. 78 % (20 -40 cm), the content of slow-acting potassium was increased by 16. 63 % (10. 20 peat derived potassium humate, mineral potash (hydrothermal method) and calcium magnesium phosphate fertilizer could ef province, increase soil potassium storage capacity and potassi um supply capacity, the proportion of upper and middle leaves, the quality of tobacco leaves and the economic situation of flue-cured toba co. Base woody peat derived potassium humate and topdressing mineral potassium fertilizer (hydrothermal method) have the best applicatic effect at 1:3. [ABSTRACT FROM AUTHOR]

Farmland management measures can have a significant impact on the carbon cycle of farmland ecosystems, which can affect soil fertility, agricultural production, and food security, and more broadly, climate change and environmental health. In this paper, the effects of different farmland management measures（fertilization methods, planting systems, and farming models）on soil organic carbon, greenhouse gas emissions, and soil microorganisms were reviewed. The increase in soil organic carbon was fastest when organic and inorganic fertilizer were applied. Moreover, there was a threshold effect between the amount of fertilizer applied and the soil carbon inventory. The application of organic fertilizer increased the CO2 emissions flux in the soil, and the application of phosphorus and potassium fertilizers reduced the globe warming potential of farmland soil more than nitrogen fertilizer. Increasing the application ratio of organic fertilizer to phosphate fertil⁃ izer was beneficial, leading to an increase in microbial richness in the soil and accumulation of microbial biomass carbon. Planting struc⁃ ture and planting density both affected the carbon storage of farmland soil, and planting structure had a significant impact on greenhouse gas emissions in farmland ecosystems. Compared with traditional monoculture planting, crop rotation and intercropping could effectively re⁃ duce the greenhouse gas emissions of farmland. At the same time, crop rotation was more conducive to increasing soil microbial diversity than continuous cropping. Conservation tillage improved the efficiency of farmland soil carbon, reduced greenhouse gas emissions, and had a positive impact on microbial activity, diversity, community structure, and carbon source utilization. Finally, we summarized the applica⁃ tion of international mainstream carbon models to farmland ecosystems, and present future development prospects. [ABSTRACT FROM AUTHOR]

[Objective] This paper aims to provide optimal fertilization guidance for high-yield cultivation of P. frutescens and promotion of new P. frutescens varieties. [ Method] The effects of nitrogen, phosphorus and potassium fertilizers on the yield of two new P. frutescens varieties ( Qisu 2 and Qisu 3) were studied by ternary quadratic orthogonal rotation design. The regression equation and model analysis were constructed to optimize the high-yield fertilization mode. [ Result] N was the most important factor influencing the yield of P. frutescens. However, the two varieties varied in response to P and K fertilization. The regression models of nitrogen (x1) , phosphorus (x2) and potassium (x3) of the yield (Y) are Y = 2219.384 + 405.410x1, +304.926x2 - 156.723x12 -78.676x22 - 214. 479x1x2, and Y =2048.806 + 319.535x1, +54.722x3 -63. 102x12 - 113. 664x22 +76.017x32 -69.069x1x2, respectively. The best fertilization ratio of N, P and K fertilizer was (286.022 ±25.288),(139.059 ±20. 819),(180. 000 ± 35. 860) kg/hm2 , and (338. 180 ± 16.617) , (80. 923 ±21.314) , (203.529 ± 24. 278 ) kg/hm2, respectively. The yield of P. frutescens could reach 2250 kg/hm2. [ Conclusion ] Compound application of NPK fertilizers functioned significantly in enhancing P. frutescens yield. [ABSTRACT FROM AUTHOR]

[Objective] The aim of this paper was to provide a theoretical relerence for the application of loss-control urea in rice production in yellow soil. [Method] Based on the same application rates of phosphorus and potassium fertilizer, the rice cultivar Leyou 58 was employed to explore the ellects of loss-control urea, with various adding amount of loss-control agent (1 %,2%,3%,4%,5%,6%), on rice yield, nutrients uptake and utilization. Meanwhile, no nitrogen and common urea fertilizer were taken as the controls. [Result] No regular changes of rice yield observed with increasing the dosage of loss-control agent, while the accumulation of nitrogen in rice grain, nitrogen, phosphorus and potassium in rice straw, and potassium in aboveground parts showed regular variation. At the same time, regular changes were also observed on rice nitrogen, phosphorus, potassium harvest index and potassium absorption efficiency. Compared with common urea treatment, six kinds of loss-control urea with the loss-control agent of 1 % -6 % could increase the rice yield in some degree, among which 1 % adding amount performed the best. The yield increasing rate was up to 12.22 %. The adding amounts of 1 % , 2 % and 6 % enables absorption of nitrogen, phosphorus, potassium by rice and accumulation in the rice seeds sufficiently to promote the yield and quality of rice. The adding amount of 3 % enhanced the accumulation of nitrogen, phosphorus and potassium in rice straw, increased the resistance and promoted the growth. [Conclusion] To sum up, the effect of urea with 1 % loss-control agent performed the best in rice production. [ABSTRACT FROM AUTHOR]