应用转录组分析不同施肥对玉米穗位叶中糖类、脂类和蛋白质代谢的影响.

[Objective] The present paper aimed to clarify the expression pattern and molecular regulation mechanism of genes related to sugar, lipid and protein metabolism in maize ear leaves under different fertilization in field conditions. [ Method] Maize cultivar 'Luyu 16' was used as the material and five treatments were set up: No fertilizer (CK), nitrogen fertilizer ( N), N + P fertilizer (NP), N + K fertilizer ( NK) and N + P + K fertilizer ( NPK). At grain filling stage, the upper part of ear leaves was harvested for transcriptome analysis using RNA-Seq techniques. The differentially expressed genes ( DEGs) under different fertilizer treatments were analyzed by Hisat2 software. [Result]GO(Gene ontology)analysis showed that DEGs related to sugar, lipid and protein metabolism were enriched in Biological process ( BP), Cell components( CC) and Molecular function( MF). Although the enriched items were the same, the number of DEGs varied under different combinations. KEGG ( Kyoto encyclopedia of genes and genomes) analysis exhibited that more DEGs were enriched in carbon metabolism, starch and sucrose metabolism, and amino acid synthesis pathways. The total number of DEGs with both I log2 FC I > 1 and P < 0. 05 involved in sugar, lipid and protein metabolism under different fertilizer treatments was 82, 29 and 84, respectively. Among them, the total number of DE Gs with I log2 FC I > 2 was 6, 24 and 17. The number of DE Gs showed the maximum in comparisons of NPvsCK and NPKvsCK but the minimum in NvsCK comparison. Further analysis of DEGs with I log2 FC I showed that these DEGs had significant differences in the expression level and expression patterns. [ Conclusion] The combined of nitrogen, potassium and phosphorus fertilizer significantly affected the transcription levels of genes related to sugar, lipid and protein amino acids metabolism in maize ear leaves. Some key genes participating in the three organic substances' metabolism were identified. The results provided molecular basis for improving the photosynthetic efficiency of maize leaves by rational fertilization in the future. [ABSTRACT FROM AUTHOR]